阅读说明：本技术 一种促进哺乳仔猪生长的动物营养液及其制备方法和应用 (Animal nutrient solution for promoting growth of suckling piglets and preparation method and application thereof ) 是由 何流琴 刘娣 周锡红 李铁军 冯艳忠 焦金真 田立余 刘西峰 殷学忠 肖龑 印遇 于 2021-02-20 设计创作，主要内容包括：本发明公开了一种促进哺乳仔猪生长的动物营养液及其制备方法和应用。本发明的动物营养液由乳酸菌、酵母菌、益生芽孢杆菌、丝氨酸、红糖、谷氨酸和清水制备而成。各原料组分的重量分数为：乳酸菌10～20份,酵母菌5～10份,益生芽孢杆菌5～10份,丝氨酸60～80份,红糖1～5份,谷氨酸1～5份和清水800～900份。在哺乳仔猪养殖时,按照每头仔猪每天2～5mL的用量,用本发明的动物营养液饲喂哺乳仔猪。本发明的动物营养液用于哺乳仔猪的养殖,能促进哺乳仔猪的生长,尤其能有效提高IUGR仔猪的生长速率,增加养殖户或企业的经济效益。(The invention discloses an animal nutrient solution for promoting growth of suckling piglets and a preparation method and application thereof. The animal nutrient solution is prepared from lactic acid bacteria, saccharomycetes, probiotic bacillus, serine, brown sugar, glutamic acid and clear water. The weight fractions of the raw material components are as follows: 10-20 parts of lactic acid bacteria, 5-10 parts of saccharomycetes, 5-10 parts of probiotic bacillus, 60-80 parts of serine, 1-5 parts of brown sugar, 1-5 parts of glutamic acid and 800-900 parts of clear water. When the suckling piglets are bred, the animal nutrient solution is used for feeding the suckling piglets according to the dosage of 2-5 mL per piglet per day. The animal nutrient solution is used for breeding the suckling piglets, can promote the growth of the suckling piglets, particularly can effectively improve the growth rate of the IUGR piglets and increase the economic benefit of farmers or enterprises.)

1. An animal nutrient solution for promoting the growth of suckling piglets is characterized in that: the animal nutrient solution is prepared from the following raw materials: lactic acid bacteria, yeast, probiotic bacillus, serine, brown sugar, glutamic acid and clear water.

2. The animal nutrient solution of claim 1, wherein: the weight fractions of the raw materials are as follows: 10-20 parts of lactic acid bacteria, 5-10 parts of saccharomycetes, 5-10 parts of probiotic bacillus, 60-80 parts of serine, 1-5 parts of brown sugar, 1-5 parts of glutamic acid and 800-900 parts of clear water.

3. Animal nutrition solution according to claim 1 or 2, characterized in that: the weight fractions of the raw materials are as follows: 13-20 parts of lactic acid bacteria, 7-10 parts of saccharomycetes, 5-8 parts of probiotic bacillus, 70-80 parts of serine, 2-5 parts of brown sugar, 1-3 parts of glutamic acid and 830-900 parts of clear water.

4. Animal nutrition solution according to claim 1 or 2, characterized in that: the weight fractions of the raw materials are as follows: 17 parts of lactic acid bacteria, 8 parts of saccharomycetes, 5 parts of probiotic bacillus, 75 parts of serine, 4 parts of brown sugar, 2 parts of glutamic acid and 870 parts of clear water.

5. Animal nutrition solution according to claim 1 or 2, characterized in that: the lactobacillus is lactobacillus rhamnosus, the yeast is saccharomyces cerevisiae, and the probiotic bacillus is bacillus amyloliquefaciens.

6. A process for preparing an animal nutrition solution according to any one of claims 1 to 5, characterized in that: the preparation method comprises the following steps:

step 1, adding all raw material components together, stirring and uniformly mixing;

and 2, carrying out facultative fermentation on the mixed material obtained by the treatment in the step 1 at the temperature of 35-38 ℃, and finishing the fermentation when the pH of the fermented material is less than or equal to 4.18, wherein the fermented product is the animal nutrient solution.

7. The method of claim 6, wherein: and the fermentation time in the step 2 is 48-72 hours.

8. Method of use of the animal nutrition solution according to any of claims 1 to 5, characterized in that: the animal nutrient solution is used for feeding suckling piglets.

9. The method of application according to claim 8, characterized in that: the suckling piglet is an IUGR piglet.

10. The method of application according to claim 8 or 9, characterized in that: the dosage of the animal nutrient solution is 2-5 mL for each suckling piglet to drink every day.

Technical Field

The invention belongs to the technical field of animal nutrition, and particularly relates to an animal nutrient solution for promoting growth of suckling piglets, and a preparation method and application thereof.

Background

With the development of society and economy, the consumption demand of people on pork and the quality requirement of pork are higher and higher, so that breeding pigs of lean type varieties are introduced to farmers or farms for breeding, and the birth weight of piglets of the lean type varieties is lower than that of common varieties and is usually less than 1 kg. In addition, the birth weight of intrauterine growth retardation (IUGR) piglets is lower.

Researches show that the piglet with lower birth weight has poorer anti-stress capability and more strict requirements on environmental temperature, nutrition level and the like. In addition, piglets with low birth weight have poor milk foraging capability and low digestion and absorption capability, so that the growth of the piglets is slow, the meat quality is reduced, and the survival rate of the piglets is low.

Therefore, the animal nutrient solution capable of promoting the growth of the suckling piglets is developed and applied to the breeding of the suckling piglets, and has great significance for the healthy development of the live pig breeding industry.

Disclosure of Invention

The invention aims to provide an animal nutrient solution which is used for breeding suckling piglets, can promote the growth of the suckling piglets and improve the growth rate of IUGR suckling piglets.

On one hand, the invention provides an animal nutrient solution for promoting the growth of suckling piglets, which is prepared from the following raw materials: lactic acid bacteria, yeast, probiotic bacillus, serine, brown sugar, glutamic acid and clear water.

Preferably, the raw materials comprise the following components in parts by weight: 10-20 parts of lactic acid bacteria, 5-10 parts of saccharomycetes, 5-10 parts of probiotic bacillus, 60-80 parts of serine, 1-5 parts of brown sugar, 1-5 parts of glutamic acid and 800-900 parts of clear water.

Preferably, the raw materials comprise the following components in parts by weight: 13-20 parts of lactic acid bacteria, 7-10 parts of saccharomycetes, 5-8 parts of probiotic bacillus, 70-80 parts of serine, 2-5 parts of brown sugar, 1-3 parts of glutamic acid and 830-900 parts of clear water.

Preferably, the raw materials comprise the following components in parts by weight: 17 parts of lactic acid bacteria, 8 parts of saccharomycetes, 5 parts of probiotic bacillus, 75 parts of serine, 4 parts of brown sugar, 2 parts of glutamic acid and 870 parts of clear water.

Preferably, the lactic acid bacteria are lactobacillus rhamnosus, the yeast is saccharomyces cerevisiae, and the probiotic bacillus is bacillus amyloliquefaciens.

In another aspect, the present invention also provides a method for preparing the animal nutrient solution, which comprises the following steps:

step 1, adding all raw material components together, stirring and uniformly mixing;

and 2, carrying out facultative fermentation on the mixed material obtained by the treatment in the step 1 at the temperature of 35-38 ℃, and finishing the fermentation when the pH of the fermented material is less than or equal to 4.18, wherein the fermented product is the animal nutrient solution.

Preferably, the fermentation time in the step 2 is 48-72 hours.

Preferably, the animal nutrient solution is used for feeding suckling piglets.

Preferably, the suckling piglet is an IUGR piglet.

Preferably, the dosage of the animal nutrient solution is 2-5 mL for each suckling piglet to drink every day.

The animal nutrient solution is used for breeding suckling piglets, can promote the growth of the suckling piglets, especially for breeding IUGR suckling piglets, can improve the growth rate of the IUGR piglets and increase the economic benefit of farmers or enterprises.

Detailed Description

The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. It should be understood that the examples are illustrative only and are not limiting upon the scope of the invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.

In the following description, all methods involved are conventional in the art unless otherwise specified. The starting materials mentioned are all those which are commercially available from the public unless otherwise specified.

Serine is a non-essential amino acid, which is in an intermediate position of amino acid metabolism, involved in the synthesis of methionine, glycine, D-serine, L-cysteine, phospholipids, purines, and the like. These substances are the prerequisite substances for the supply of synthetic proteins, amino acids, nucleotides, neurotransmitters and serine-derived lipids at different stages of animal development.

The lactobacillus, the microzyme and the probiotic bacillus are beneficial bacteria or fungi in animal bodies, can adjust the immune function of animal bodies and the flora balance in animal intestinal tracts, promote the absorption of nutrient substances and improve the feed conversion rate.

According to the invention, serine, brown sugar and glutamic acid are added together and mixed uniformly, then the mixed material is added into clear water, and the mixed liquid is fermented by lactic acid bacteria, saccharomycetes and probiotic bacillus to obtain the animal nutrient solution for promoting the growth of suckling piglets. The Lactobacillus can be at least one of Lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus acidophilus (Lactobacillus acidophilus), Lactobacillus paracasei (Lactobacillus paracasei) or Lactobacillus rhamnosus (Lactobacillus rhamnosus), and preferably Lactobacillus rhamnosus. The yeast can be Candida utilis (Candida utilis) and/or Saccharomyces cerevisiae (Saccharomyces cerevisiae), and more preferably Saccharomyces cerevisiae. The probiotic Bacillus can adopt Bacillus subtilis and/or Bacillus amyloliquefaciens, and further preferably Bacillus amyloliquefaciens. The animal nutrient solution is used for breeding suckling piglets, can promote the growth of the suckling piglets, is particularly used for breeding IUGR suckling piglets, can improve the growth rate of the IUGR piglets, and increases the economic benefit of farmers or enterprises.

In a specific embodiment of the invention, serine, brown sugar, glutamic acid, lactic acid bacteria, yeast, probiotic bacillus and clear water are used as raw materials to prepare the animal nutrient solution capable of promoting the growth of suckling piglets, and the specific preparation process comprises the following steps:

1) according to the preset proportion, lactic acid bacteria, saccharomycetes, probiotic bacillus, serine, brown sugar, glutamic acid and clear water are added and mixed evenly to obtain a liquid material. The preferred weight ratio of the raw material components is 10-20 parts of lactic acid bacteria, 5-10 parts of saccharomycetes, 5-10 parts of probiotic bacillus, 60-80 parts of serine, 1-5 parts of brown sugar, 1-5 parts of glutamic acid and 800-900 parts of clear water. Wherein the content of lactobacillus is not less than 2.0 × 10¹¹cfu/mL, the bacteria content of the microzyme is more than or equal to 4.0 multiplied by 10⁹cfu/mL, the bacterium content of the probiotic bacillus is more than or equal to 3.0 multiplied by 10¹⁰cfu/mL。

2) Carrying out facultative fermentation on the liquid material obtained in the step 1) for 48-72 hours at the temperature of 35-38 ℃, and finishing the fermentation when the pH value of the liquid material is less than or equal to 4.18, wherein the fermentation product is the animal nutrient solution.

When the animal nutrient solution is applied, the serine content in the animal nutrient solution is adjusted to be 0.1-0.8% by mass, and then the serine content is added into drinking water of suckling piglets or directly poured into mouths of the piglets, preferably directly poured into the mouths of the piglets, wherein the dosage of the animal nutrient solution is preferably 2-5 mL per suckling piglet per day.

To help better understand the technical solution of the present invention, the following examples are provided for illustrating the preparation process and application method of the animal nutrient solution of the present invention.

Example one

The raw materials of the animal nutrient solution of the embodiment comprise, by weight, 10 parts of lactobacillus rhamnosus, 5 parts of saccharomyces cerevisiae, 10 parts of bacillus amyloliquefaciens, 60 parts of serine, 1 part of brown sugar, 5 parts of glutamic acid and 800 parts of clear water. The inventor prepares three kinds of single-strain bacterial liquids, firstly prepares lactobacillus rhamnosus, saccharomyces cerevisiae and bacillus amyloliquefaciens into seed liquids respectively, then transfers the seed liquids into corresponding liquid culture media, and obtains the bacterial liquids of the single strains through fermentation culture. The bacterial contents of three single-strain bacterial liquids are respectively as follows: the content of Lactobacillus rhamnosus is not less than 2.0 × 10¹¹cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 4.0 multiplied by 10⁹cfu/mL, the bacterium content of the bacillus amyloliquefaciens is more than or equal to 3.0 multiplied by 10¹⁰cfu/mL。

The animal nutrient solution of this example was prepared as follows:

1) adding lactobacillus rhamnosus liquid, saccharomyces cerevisiae liquid, bacillus amyloliquefaciens liquid, serine, brown sugar, glutamic acid and clear water into the same fermentation tank, and uniformly mixing to obtain a liquid material.

2) Carrying out facultative fermentation on the liquid material obtained in the step 1) for 48 hours at the temperature of 35-38 ℃, and finishing the fermentation when the pH value of the liquid material is less than or equal to 4.18, wherein the fermentation product is the animal nutrient solution 1.

The application method of the animal nutrient solution 1 comprises the following steps: after the suckling piglets are born, the serine content in the animal nutrient solution 1 is adjusted to be 0.1 percent by mass, and the animal nutrient solution is poured into the mouths of the piglets according to the dosage of 2mL per day for each piglet.

Example two

The raw materials of the animal nutrient solution comprise, by weight, 13 parts of lactobacillus rhamnosus, 7 parts of saccharomyces cerevisiae, 8 parts of bacillus amyloliquefaciens, 70 parts of serine, 2 parts of brown sugar, 3 parts of glutamic acid and 830 parts of clear water. The inventor prepares three kinds of single-strain bacterial liquids, firstly prepares lactobacillus rhamnosus, saccharomyces cerevisiae and bacillus amyloliquefaciens into seed liquids respectively, then transfers the seed liquids into corresponding liquid culture media, and obtains the bacterial liquids of the single strains through fermentation culture. The bacterial contents of three single-strain bacterial liquids are respectively as follows: the content of Lactobacillus rhamnosus is not less than 2.0 × 10¹¹cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 4.0 multiplied by 10⁹cfu/mL, the bacterium content of the bacillus amyloliquefaciens is more than or equal to 3.0 multiplied by 10¹⁰cfu/mL。

The animal nutrient solution of this example was prepared as follows:

1) adding lactobacillus rhamnosus liquid, saccharomyces cerevisiae liquid, bacillus amyloliquefaciens liquid, serine, brown sugar, glutamic acid and clear water into the same fermentation tank, and uniformly mixing to obtain a liquid material.

2) Carrying out facultative fermentation on the liquid material obtained in the step 1) for 54 hours at the temperature of 35-38 ℃, and finishing the fermentation when the pH value of the liquid material is less than or equal to 4.10, wherein the fermentation product is the animal nutrient solution 2.

The application method of the animal nutrient solution 2 comprises the following steps: after the suckling piglets are born, the serine content in the animal nutrient solution 2 is adjusted to be 0.2 percent by mass, and the serine is poured into the mouths of the piglets according to the dosage of 3mL per day for each piglet.

EXAMPLE III

The raw materials of the animal nutrient solution of the embodiment comprise, by weight, 17 parts of lactobacillus rhamnosus, 8 parts of saccharomyces cerevisiae, 5 parts of bacillus amyloliquefaciens, 75 parts of serine, 4 parts of brown sugar, 2 parts of glutamic acid and 870 parts of clear water. The inventor prepares three single-strain bacterial liquids, firstly prepares lactobacillus rhamnosus, saccharomyces cerevisiae and bacillus amyloliquefaciens into seed liquids respectively, and then transfers the seed liquids into corresponding liquid culture mediaAnd fermenting and culturing to obtain the bacterial liquid of a single bacterial strain. The bacterial contents of three single-strain bacterial liquids are respectively as follows: the content of Lactobacillus rhamnosus is not less than 2.0 × 10¹¹cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 4.0 multiplied by 10⁹cfu/mL, the bacterium content of the bacillus amyloliquefaciens is more than or equal to 3.0 multiplied by 10¹⁰cfu/mL。

The animal nutrient solution of this example was prepared as follows:

1) adding lactobacillus rhamnosus liquid, saccharomyces cerevisiae liquid, bacillus amyloliquefaciens liquid, serine, brown sugar, glutamic acid and clear water into the same fermentation tank, and uniformly mixing to obtain a liquid material.

2) Carrying out facultative fermentation on the liquid material obtained in the step 1) for 60 hours at the temperature of 35-38 ℃, and finishing the fermentation when the pH value of the liquid material is less than or equal to 4.06, wherein the fermentation product is the animal nutrient solution 3.

The application method of the animal nutrient solution 3 comprises the following steps: after the suckling piglets are born, the serine content in the animal nutrient solution 3 is adjusted to be 0.4 percent by mass, and the serine is poured into the mouths of the piglets according to the dosage of 4mL per day for each piglet.

Example four

The raw materials of the animal nutrient solution of the embodiment comprise, by weight, 20 parts of lactobacillus rhamnosus, 10 parts of saccharomyces cerevisiae, 7 parts of bacillus amyloliquefaciens, 80 parts of serine, 5 parts of brown sugar, 1 part of glutamic acid and 900 parts of clear water. The inventor prepares three kinds of single-strain bacterial liquids, firstly prepares lactobacillus rhamnosus, saccharomyces cerevisiae and bacillus amyloliquefaciens into seed liquids respectively, then transfers the seed liquids into corresponding liquid culture media, and obtains the bacterial liquids of the single strains through fermentation culture. The bacterial contents of three single-strain bacterial liquids are respectively as follows: the content of Lactobacillus rhamnosus is not less than 2.0 × 10¹¹cfu/mL, the bacteria content of the saccharomyces cerevisiae is more than or equal to 4.0 multiplied by 10⁹cfu/mL, the bacterium content of the bacillus amyloliquefaciens is more than or equal to 3.0 multiplied by 10¹⁰cfu/mL。

The animal nutrient solution of this example was prepared as follows:

1) adding lactobacillus rhamnosus liquid, saccharomyces cerevisiae liquid, bacillus amyloliquefaciens liquid, serine, brown sugar, glutamic acid and clear water into the same fermentation tank, and uniformly mixing to obtain a liquid material.

2) Carrying out facultative fermentation on the liquid material obtained in the step 1) for 72 hours at the temperature of 35-38 ℃, and finishing the fermentation when the pH value of the liquid material is less than or equal to 4.00, wherein the fermentation product is the animal nutrient solution 4.

The application method of the animal nutrient solution 4 comprises the following steps: after the suckling piglets are born, the serine content in the animal nutrient solution 4 is adjusted to be 0.8 percent by mass, and the serine is poured into the mouths of the piglets according to the dosage of 5mL per day for each piglet.

In order to help better understand the technical scheme of the invention, the following provides a test example of feeding the suckling piglets for illustrating the application effect of the invention.

The first test example: influence of animal nutrient solution on growth of suckling piglet with intrauterine growth retardation

50 IUGR piglets with the birth weight of about 0.99kg are selected just after delivery. The IUGR suckling piglets were randomly divided into 5 groups, including 4 trial groups and 1 control group, each group with 10 replicates, using a single factor random trial design.

The IUGR piglets are fed with 1-4 animal nutrient solutions prepared by the invention every day from the 1 st day of birth, and the test period is 26 days while sucking breast milk normally. The specific feeding method of the nutrient solution for the 4 kinds of animals comprises the following steps: firstly, adjusting the serine content in the animal nutrient solution 1 to be 0.1 percent by mass, and pouring the animal nutrient solution into the mouths of piglets according to the dosage of 2mL per day for each piglet; firstly, adjusting the serine content in the animal nutrient solution 2 to be 0.2 mass percent, and pouring the animal nutrient solution into the mouths of piglets according to the dosage of 3mL per day for each piglet; firstly, adjusting the serine content in the animal nutrient solution 3 to be 0.4 percent by mass, and pouring the animal nutrient solution into the mouths of piglets according to the dosage of 4mL per piglet per day; firstly, the serine content in the animal nutrient solution 4 is adjusted to be 0.8 percent by mass, and the animal nutrient solution is poured into the mouths of piglets according to the dosage of 5mL per day for each piglet. The IUGR piglets of the control group use normal saline instead of the animal nutrient solution of the invention, and the rest of daily management is the same as that of the test group.

Each IUGR piglet was housed separately in different sows, all sows were fed the same ration, fed individually, and had free access to water. The piglets were weighed and recorded before drenching on days 1, 4, 7, 11, 15, 19, 21, 23, 25 and 26, and the results are shown in table 1. In addition, the health and survival of piglets were recorded and after the experiment was completed, the average body weight and survival rate of piglets per group of IUGR were calculated. The results are shown in Table 2.

TABLE 1

As can be seen from the data in Table 1, the weights of the IUGR piglets in the test group from the beginning of day 4 to the end of the test group were all higher than those in the control group, indicating that the growth of the IUGR piglets was promoted by feeding the animal nutrient solution of the present invention.

TABLE 2

The data in table 2 show that the average daily gain and the survival rate of piglets in the test group are both obviously improved compared with the control group, and the survival rate of IUGR piglets is improved from 20.41% to 100%. The animal nutrient solution can promote the growth of IUGR suckling piglets and improve the growth performance of IUGR piglets.

Test example two: effect of animal nutrient solution on growth of normal suckling piglet

50 normal piglets with birth weight of about 1.30kg are selected just after delivery. Normal piglets were randomly divided into 5 groups, including 4 trial groups and 1 control group, with 10 replicates per group, using a single-factor random trial design.

From day 1 of birth, piglets in 4 test groups fed 1-4 animal nutrient solutions prepared by the invention every day while sucking breast milk normally, and the test period is 26 days. The specific feeding method of the nutrient solution for the 4 kinds of animals comprises the following steps: firstly, adjusting the serine content in the animal nutrient solution 1 to be 0.1 percent by mass, and pouring the animal nutrient solution into the mouths of piglets according to the dosage of 2mL per day for each piglet; firstly, adjusting the serine content in the animal nutrient solution 2 to be 0.2 mass percent, and pouring the animal nutrient solution into the mouths of piglets according to the dosage of 3mL per day for each piglet; firstly, adjusting the serine content in the animal nutrient solution 3 to be 0.4 percent by mass, and pouring the animal nutrient solution into the mouths of piglets according to the dosage of 4mL per piglet per day; firstly, the serine content in the animal nutrient solution 4 is adjusted to be 0.8 percent by mass, and the animal nutrient solution is poured into the mouths of piglets according to the dosage of 5mL per day for each piglet. Normal piglets in the control group were treated with normal saline instead of the animal nutrient solution of the present invention, and the other daily management was the same as in the test group.

Each normal piglet is fostered in different sows, and all sows are fed with the same daily ration and fed in a single fence with free drinking water. The piglets were weighed and recorded before drenching on days 1, 4, 7, 11, 15, 19, 21, 23, 25 and 26, and the results are shown in table 3. In addition, the health and survival of piglets were recorded and after the experiment was completed, the average weight and survival rate of piglets per group were calculated. The results are shown in Table 4.

TABLE 3

As can be seen from the data in Table 3, the weight of the piglets in the test group is higher than that in the control group from the 4 th day to the end of the test, which indicates that the feeding of the animal nutrient solution of the invention can promote the growth of normal piglets.

TABLE 4

The data in table 4 show that the average daily gain and the survival rate of piglets in the test group are both obviously improved compared with the control group, and the survival rate of normal piglets is improved from 80.3% to 100%. The animal nutrient solution can promote the growth of normal suckling piglets and improve the growth performance of the normal piglets.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent structural changes made by using the contents of the present specification, or any other related technical fields directly or indirectly, are included in the scope of the present invention.