阅读说明：本技术 一种心脏脱细胞基质复合温敏凝胶及其应用 (Heart acellular matrix composite temperature-sensitive gel and application thereof ) 是由 孙广炜 刘洋 张英 赵姗 于 2019-11-22 设计创作，主要内容包括：本发明涉及一种心脏脱细胞基质复合温敏凝胶,其特征在于此复合温敏凝胶由壳聚糖温敏凝胶和猪心脏脱细胞基质颗粒(粒径为10-100μm)所构成。本发明还提供上述复合温敏凝胶的制备方法,步骤为：(1)制备壳聚糖溶液,在冰浴及搅拌条件下将β-甘油磷酸钠溶液逐滴加入到壳聚糖溶液中,制备得到低温的壳聚糖温敏凝胶成胶溶液；(2)制备猪心脏脱细胞基质颗粒,粒径为10-100μm；(3)将猪心脏脱细胞基质颗粒加入上述低温的壳聚糖温敏凝胶成胶溶液,颗粒浓度为0.2-6％(w/v,g/ml),从而得到心脏脱细胞基质复合温敏凝胶成胶溶液；(4)低温的心脏脱细胞基质复合温敏凝胶成胶溶液在处于33-37℃条件时可快速转变为凝胶态。该心脏脱细胞基质复合温敏凝胶成胶溶液可通过注射到达心脏受损部位,原位快速形成复合凝胶,起到修复受损心肌组织的效果。(The invention relates to a heart acellular matrix composite temperature-sensitive gel, which is characterized by consisting of chitosan temperature-sensitive gel and pig heart acellular matrix particles (the particle size is 10-100 mu m). The invention also provides a preparation method of the composite temperature-sensitive gel, which comprises the following steps: (1) preparing a chitosan solution, dropwise adding a beta-sodium glycerophosphate solution into the chitosan solution under the conditions of ice bath and stirring, and preparing a low-temperature chitosan temperature-sensitive gel forming solution; (2) preparing pig heart acellular matrix particles with the particle size of 10-100 mu m; (3) adding the pig heart acellular matrix particles into the low-temperature chitosan temperature-sensitive gel forming solution, wherein the particle concentration is 0.2-6% (w/v, g/ml), so as to obtain the heart acellular matrix composite temperature-sensitive gel forming solution; (4) the low-temperature heart acellular matrix composite temperature-sensitive gel forming solution can be quickly converted into a gel state at the temperature of 33-37 ℃. The gel forming solution of the heart acellular matrix composite temperature-sensitive gel can reach the damaged part of the heart through injection, and the composite gel is rapidly formed in situ, so that the effect of repairing the damaged myocardial tissue is achieved.)