阅读说明：本技术 多级反应微流道结构、微流控芯片和非均相反应方法 (Multi-stage reaction micro-channel structure, micro-fluidic chip and heterogeneous reaction method ) 是由 王超 蒋志强 李嘉辉 于 2021-01-13 设计创作，主要内容包括：本申请提供了多级反应微流道结构、微流控芯片和非均相反应方法,多级反应微流道结构包括：连续外三角扩张聚焦单元、主动阀定量均匀控制单元和多级非均相反应池单元；连续外三角扩张聚焦单元包括：连续外三角扩张聚焦流道和连续液相流道；主动阀定量均匀控制单元包括第一主动阀、第二主动阀和第三主动阀,第一主动阀包括内置阀塞、气相通道和气体缓冲室；连续液相流道内壁设有内置阀塞；多级非均相反应池单元包括一级非均相反应池单元和二级非均相反应池单元。本申请解决了如何设计一种微流控装置和操作工艺,使其能够在生成高分散液滴、颗粒的基础上,实现快速准确的定量控制进行精确高效非均相反应,且提高反应的充分性的技术问题。(The application provides a multistage reaction microchannel structure, a microfluidic chip and a heterogeneous reaction method, wherein the multistage reaction microchannel structure comprises: the device comprises a continuous outer triangular expansion focusing unit, a driving valve quantitative uniform control unit and a multi-stage heterogeneous reaction tank unit; the continuous outer triangular expanding focusing unit comprises: the continuous outer triangular expansion focusing flow channel and the continuous liquid phase flow channel; the active valve quantitative uniform control unit comprises a first active valve, a second active valve and a third active valve, wherein the first active valve comprises a built-in valve plug, a gas phase channel and a gas buffer chamber; the inner wall of the continuous liquid phase flow passage is provided with a built-in valve plug; the multistage heterogeneous reaction pool unit comprises a first-stage heterogeneous reaction pool unit and a second-stage heterogeneous reaction pool unit. The application solves the technical problems that how to design a microfluidic device and an operation process to enable the microfluidic device to realize rapid and accurate quantitative control to carry out accurate and efficient heterogeneous reaction on the basis of generating high-dispersion liquid drops and particles and improve the reaction sufficiency.)

1. Multistage reaction microchannel structure, its characterized in that includes: the device comprises a continuous outer triangular expansion focusing unit, a driving valve quantitative uniform control unit and a multi-stage heterogeneous reaction tank unit;

the continuous outer triangular expanding focusing unit includes: the continuous liquid phase sampling device comprises a continuous liquid phase sampling port, a continuous outer triangular expanding focusing flow channel, a continuous liquid phase flow channel, a gas inlet and a gas inlet flow channel;

the continuous liquid phase sample inlet is communicated with the liquid inlet end of the continuous outer triangular expanding and focusing flow channel, the liquid inlet end of the continuous liquid phase flow channel is communicated with the liquid outlet end of the continuous outer triangular expanding and focusing flow channel, the gas outlet end of the gas inlet flow channel is communicated with the continuous liquid phase flow channel, and the gas inlet end of the gas inlet flow channel is communicated with the gas inlet;

the multistage heterogeneous reaction pool unit comprises a first-stage heterogeneous reaction pool unit and a second-stage heterogeneous reaction pool unit;

the first-stage heterogeneous reaction pool unit comprises: the device comprises a first reaction liquid phase sample inlet, a first reaction liquid phase flow channel, a first heterogeneous reaction pool and a first liquid outlet flow channel;

the liquid inlet end of the first reaction liquid phase flow channel is communicated with the first reaction liquid phase sample inlet, the liquid outlet end of the first reaction liquid phase flow channel is communicated with the liquid inlet end of the first heterogeneous reaction pool, the liquid outlet end of the continuous liquid phase flow channel is communicated with the liquid inlet end of the first heterogeneous reaction pool, and the liquid outlet end of the heterogeneous reaction pool is communicated with the liquid inlet end of the first liquid outlet flow channel;

the second-stage heterogeneous reaction cell unit comprises: the device comprises a first reaction liquid phase sample inlet, a first reaction liquid phase flow channel, a first heterogeneous reaction pool, a first liquid outlet flow channel and a mixed phase sample outlet;

the liquid inlet end of the second reaction liquid phase flow channel is communicated with the second reaction liquid phase sample inlet, the liquid outlet end of the second reaction liquid phase flow channel is communicated with the liquid inlet end of the second heterogeneous reaction pool, the liquid outlet end of the first liquid outlet flow channel is communicated with the liquid inlet end of the second heterogeneous reaction pool, the liquid outlet end of the heterogeneous reaction pool is communicated with the second liquid outlet flow channel, and the liquid outlet end of the second liquid outlet flow channel is communicated with the mixed phase sample outlet;

the active valve quantitative uniform control unit comprises: the continuous liquid phase flow channel is provided with a first liquid outlet flow channel, a second liquid outlet flow channel and a third liquid outlet flow channel;

the first active valve includes: a valve plug, a gas phase sample inlet, a gas phase channel and a gas buffer chamber are arranged in the gas buffer chamber;

the built-in valve plug is arranged in the continuous liquid phase flow channel, the gas outlet end of the gas phase channel is communicated with the gas buffer chamber, the gas inlet end of the gas phase channel is communicated with the gas phase sample inlet, and the gas buffer chamber corresponds to the built-in valve plug;

the second active valve and the third active valve are both identical in structure to the first active valve.

2. The multi-stage reaction microchannel structure of claim 1, wherein the continuous outer triangular expanding focusing channel is in a spiral shape;

the liquid inlet end of the continuous outer triangular expanding focusing flow channel is positioned at the center of the spiral shape;

the liquid outlet end of the continuous outer triangular expansion focusing flow passage is positioned at the outer side of the spiral shape.

3. The multi-stage reactive micro flow channel structure of claim 1, wherein the number of the first active valves is at least one, and the number of the second active valves is at least one.

4. The multi-stage reaction micro flow channel structure of claim 1, wherein the built-in valve plug comprises a trapezoidal valve block and a rectangular valve block;

the trapezoidal valve block is arranged on one side, far away from the gas buffer chamber, of the inner wall of the continuous liquid phase flow channel, and the bottom surface of the trapezoidal valve block is attached to the inner wall of the continuous liquid phase flow channel;

the rectangle valve block set up in the continuous liquid phase runner inner wall is close to in one side of gas buffer room, the rectangle valve block with trapezoidal valve block staggered distribution, just the rectangle valve block with the corresponding lateral wall of trapezoidal valve block is located on the same cross-section of continuous liquid phase runner.

5. The multi-stage reaction micro flow channel structure of claim 4,

the gas buffer chamber and the continuous liquid phase flow channel are both made of deformable materials, the gas buffer chamber does not deform in a non-inflation state, the gas buffer chamber expands in an inflation state and is abutted against one side of the continuous liquid phase flow channel, so that the inner wall of the continuous liquid phase flow channel is fully contacted with the built-in valve plug, and the continuous liquid phase flow channel is blocked.

6. The multi-stage reaction micro flow channel structure of claim 1, wherein the continuous liquid phase flow channel, the gas phase channel and the reaction liquid phase flow channel are rectangular in cross section, and the height of each flow channel is uniform and is 100 μm to 200 μm.

7. The multi-stage reaction microchannel structure of claim 1, wherein the side walls of the first heterogeneous reaction cell and the second heterogeneous reaction cell are both circular side walls.

8. A microfluidic chip comprising a chip body and the multi-stage reaction microchannel structure according to any one of claims 1 to 7;

the multi-stage reaction micro-channel structure is arranged in the chip body.

9. The microfluidic chip according to claim 8, wherein the chip body comprises a substrate and a cover plate;

the multistage reaction micro-channel structure is arranged on the upper surface of the substrate;

the apron cover in the upper surface of base plate, just continuous liquid phase introduction port the gas phase introduction port reaction liquid phase introduction port with mixed phase appearance mouth all link up in the apron.

10. The microfluidic chip according to claim 8, further comprising a delivery device and an extraction device;

the conveying device comprises a first conveying pump communicated with the continuous liquid phase sample inlet, a second conveying pump communicated with the gas phase sample inlet of the first active valve, a third conveying pump communicated with the first reaction liquid phase sample inlet, a fourth conveying pump communicated with the gas phase sample inlet of the second active valve, a fifth conveying pump communicated with the second reaction liquid phase sample inlet, a sixth conveying pump communicated with the gas phase sample inlet of the third active valve and a seventh conveying pump communicated with the gas inlet;

the extraction device is communicated with the mixed phase sample outlet.

11. A heterogeneous reaction method applied to the multi-stage reaction micro flow channel structure of any one of claims 1 to 7, comprising the steps of:

uniformly and stably dispersing the microsphere suspension liquid through a continuous outer triangular expansion focusing flow channel, flowing the microsphere suspension liquid into a continuous liquid phase channel, and introducing the microsphere suspension liquid into a first heterogeneous reaction tank through a continuous liquid phase flow channel;

the flow of the continuous liquid phase flow channel is adjusted through a first active valve of the active valve quantitative uniform control unit;

a reaction liquid enters a first heterogeneous reaction tank through a first reaction liquid phase flow channel, enters the first heterogeneous reaction tank after being in short contact with a first heterogeneous microsphere suspension liquid for reaction, and is transmitted to a second heterogeneous reaction tank through a first liquid outlet flow channel;

the flow of the first liquid outlet flow channel is regulated by a second active valve of the active valve quantitative uniform control unit;

the other reaction liquid enters a second heterogeneous reaction tank through a second reaction liquid phase flow channel and fully reacts with substances in the second heterogeneous reaction tank, and the reacted substances are discharged from a second liquid outlet flow channel;

and the flow of the second liquid outlet flow channel is regulated by a third driving valve of the driving valve quantitative and uniform control unit.

Technical Field

The application relates to the technical field of microfluidics, in particular to a multistage reaction microchannel structure, a microfluidic chip and a heterogeneous reaction method.

Background

With the development of science and technology, more and more fields (energy, immunity, biochemistry, and the like) need to use a miniaturized reaction means to perform highly dispersed micro precise operations, and the micro-fluidic technology has attracted extensive attention because of being capable of realizing a lot of micro-processing and micro-operation which are difficult to be completed. Microfluidics is a manipulation of minute particles (or samples) that cannot be achieved by some conventional methods using microchannels and devices. The method can integrate biological detection, a series of biochemical reactions and preparation of various samples on a tiny chip for special operation, and has wide application prospect in multiple fields.

At present, the conventional preparation process of liquid drops or microsphere particles mainly adopts a large-scale mechanical stirring method, so that the microsphere particles with specific particle size cannot be accurately screened, the particle dispersibility is low, and the high-efficiency reaction cannot be ensured when the number of liquid drops (or particles) participating in the reaction is too large or too small. The liquid drops (or particles) can be uniformly dispersed by a micro-fluidic system with a special structure, effective and sufficient reaction is carried out after quantitative control, and the efficiency and the success rate of experiments can be effectively improved.

There are many methods of generating (or wrapping droplets of particles) using microfluidics, which actively require an external magnetic field and an electric field; the passive mode usually adopts dean flow, does not need energy input, and the device is simple and convenient, easy to maintain and small in size. Passive dean flow is currently one of the most effective means for microfluidic focusing of droplets (or particle-encapsulated droplets) due to its simplicity and convenience of operation, uniformity and high efficiency. By passive dean flow focusing, the micro-ball and liquid drop scattered in disorder can be focused to form micro-ball and liquid drop queue arranged at equal intervals at specific positions in the micro-channel. Although the dispersion of the liquid drops (or particles) is achieved to a certain extent, a spiral bent flow channel with a certain length is required to achieve the purpose, and accurate quantitative control is difficult to perform.

Therefore, how to design a microfluidic device and an operation process to realize fast and accurate quantitative control to perform accurate and efficient heterogeneous reaction on the basis of generating highly dispersed droplets and particles and improve the sufficiency of the reaction is one of the problems to be solved in the art.

Disclosure of Invention

The application aims to provide a multistage reaction micro-channel structure, a micro-fluidic chip and a heterogeneous reaction method, which are used for solving the technical problems that how to design a micro-fluidic device and an operation process, the micro-fluidic device and the operation process can realize rapid and accurate quantitative control to carry out accurate and efficient heterogeneous reaction on the basis of generating highly dispersed liquid drops and particles, and the reaction sufficiency is improved.

In order to solve the above problems, the present application provides a multistage reaction microchannel structure comprising: the device comprises a continuous outer triangular expansion focusing unit, a driving valve quantitative uniform control unit and a multi-stage heterogeneous reaction tank unit;

the continuous outer triangular expanding focusing unit includes: the continuous liquid phase sampling device comprises a continuous liquid phase sampling port, a continuous outer triangular expanding focusing flow channel, a continuous liquid phase flow channel, a gas inlet and a gas inlet flow channel;

the continuous liquid phase sample inlet is communicated with the liquid inlet end of the continuous external triangular expanding and focusing flow channel, the liquid inlet end of the continuous liquid phase flow channel is communicated with the liquid outlet end of the continuous external triangular expanding and focusing flow channel, the gas outlet end of the gas inlet flow channel is communicated with the continuous liquid phase flow channel, and the gas inlet end of the gas inlet flow channel is communicated with the gas inlet;

the multistage heterogeneous reaction pool unit comprises a first-stage heterogeneous reaction pool unit and a second-stage heterogeneous reaction pool unit;

the first-stage heterogeneous reaction pool unit comprises: the device comprises a first reaction liquid phase sample inlet, a first reaction liquid phase flow channel, a first heterogeneous reaction pool and a first liquid outlet flow channel;

the liquid inlet end of the first reaction liquid phase flow channel is communicated with the first reaction liquid phase sample inlet, the liquid outlet end of the first reaction liquid phase flow channel is communicated with the liquid inlet end of the first heterogeneous reaction pool, the liquid outlet end of the continuous liquid phase flow channel is communicated with the liquid inlet end of the first heterogeneous reaction pool, and the liquid outlet end of the heterogeneous reaction pool is communicated with the liquid inlet end of the first liquid outlet flow channel;

the second-stage heterogeneous reaction cell unit comprises: the device comprises a first reaction liquid phase sample inlet, a first reaction liquid phase flow channel, a first heterogeneous reaction pool, a first liquid outlet flow channel and a mixed phase sample outlet;

the liquid inlet end of the second reaction liquid phase flow channel is communicated with the second reaction liquid phase sample inlet, the liquid outlet end of the second reaction liquid phase flow channel is communicated with the liquid inlet end of the second heterogeneous reaction pool, the liquid outlet end of the first liquid outlet flow channel is communicated with the liquid inlet end of the second heterogeneous reaction pool, the liquid outlet end of the heterogeneous reaction pool is communicated with the second liquid outlet flow channel, and the liquid outlet end of the second liquid outlet flow channel is communicated with the mixed phase sample outlet;

the active valve quantitative uniform control unit comprises: the continuous liquid phase flow channel is provided with a first liquid outlet flow channel, a second liquid outlet flow channel and a third liquid outlet flow channel;

the first active valve includes: a valve plug, a gas phase sample inlet, a gas phase channel and a gas buffer chamber are arranged in the gas buffer chamber;

the built-in valve plug is arranged in the continuous liquid phase flow channel, the gas outlet end of the gas phase channel is communicated with the gas buffer chamber, the gas inlet end of the gas phase channel is communicated with the gas phase sample inlet, and the gas buffer chamber corresponds to the built-in valve plug;

the second active valve and the third active valve are both identical in structure to the first active valve.

Furthermore, the continuous outer triangular expanding focusing flow channel is spiral;

the liquid inlet end of the continuous outer triangular expanding focusing flow channel is positioned at the center of the spiral shape;

the liquid outlet end of the continuous outer triangular expansion focusing flow passage is positioned at the outer side of the spiral shape.

Further, the number of the first active valves is at least one, and the number of the second active valves is at least one.

Further, the built-in valve plug comprises a trapezoid valve block and a rectangular valve block;

the trapezoidal valve block is arranged on one side, far away from the gas buffer chamber, of the inner wall of the continuous liquid phase flow channel, and the bottom surface of the trapezoidal valve block is attached to the inner wall of the continuous liquid phase flow channel;

the rectangle valve block set up in the continuous liquid phase runner inner wall is close to in one side of gas buffer room, the rectangle valve block with trapezoidal valve block staggered distribution, just the rectangle valve block with the corresponding lateral wall of trapezoidal valve block is located on the same cross-section of continuous liquid phase runner.

Furthermore, the gas buffer chamber and the continuous liquid phase flow channel are both made of deformable materials, the gas buffer chamber does not deform in a non-inflation state, the gas buffer chamber expands in an inflation state and abuts against one side of the continuous liquid phase flow channel, so that the inner wall of the continuous liquid phase flow channel is in full contact with the built-in valve plug, and the continuous liquid phase flow channel is blocked.

Furthermore, the cross sections of the continuous liquid phase flow channel, the gas phase channel and the reaction liquid phase flow channel are rectangular, the heights of the various flow channels are uniform, and the heights of the various flow channels are all 100-200 mu m.

Further, the side walls of the first heterogeneous reaction tank and the second heterogeneous reaction tank are both circular side walls.

The application also provides a micro-fluidic chip which comprises a chip body and the multi-stage reaction micro-channel structure;

the multi-stage reaction micro-channel structure is arranged in the chip body.

Further, the chip body comprises a substrate and a cover plate;

the multistage reaction micro-channel structure is arranged on the upper surface of the substrate;

the apron cover in the upper surface of base plate, just continuous liquid phase introduction port the gas phase introduction port reaction liquid phase introduction port with mixed phase appearance mouth all link up in the apron.

Further, the device also comprises a conveying device and an extracting device;

the conveying device comprises a first conveying pump communicated with the continuous liquid phase sample inlet, a second conveying pump communicated with the gas phase sample inlet of the first active valve, a third conveying pump communicated with the first reaction liquid phase sample inlet, a fourth conveying pump communicated with the gas phase sample inlet of the second active valve, a fifth conveying pump communicated with the second reaction liquid phase sample inlet, a sixth conveying pump communicated with the gas phase sample inlet of the third active valve and a seventh conveying pump communicated with the gas inlet;

the extraction device is communicated with the mixed phase sample outlet.

The application also provides a heterogeneous reaction method, which is applied to the multistage reaction micro-channel structure and comprises the following steps:

uniformly and stably dispersing the microsphere suspension liquid through a continuous outer triangular expansion focusing flow channel, flowing the microsphere suspension liquid into a continuous liquid phase channel, and introducing the microsphere suspension liquid into a first heterogeneous reaction tank through a continuous liquid phase flow channel;

the flow of the continuous liquid phase flow channel is adjusted through a first active valve of the active valve quantitative uniform control unit;

a reaction liquid enters a first heterogeneous reaction tank through a first reaction liquid phase flow channel, enters the first heterogeneous reaction tank after being in short contact with a first heterogeneous microsphere suspension liquid for reaction, and is transmitted to a second heterogeneous reaction tank through a first liquid outlet flow channel;

the flow of the first liquid outlet flow channel is regulated by a second active valve of the active valve quantitative uniform control unit;

the other reaction liquid enters a second heterogeneous reaction tank through a second reaction liquid phase flow channel and fully reacts with substances in the second heterogeneous reaction tank, and the reacted substances are discharged from a second liquid outlet flow channel;

and the flow of the second liquid outlet flow channel is regulated by a third driving valve of the driving valve quantitative and uniform control unit.

Compared with the prior art, the embodiment of the application has the advantages that:

the application provides a multistage reaction microchannel structure, includes: the device comprises a continuous outer triangular expansion focusing unit, a driving valve quantitative uniform control unit and a multi-stage heterogeneous reaction tank unit; the continuous outer triangular expanding focusing unit comprises: the continuous liquid phase sampling device comprises a continuous liquid phase sampling port, a continuous outer triangular expanding focusing flow channel, a continuous liquid phase flow channel, a gas inlet and a gas inlet flow channel; the continuous liquid phase sample inlet is communicated with the liquid inlet end of the continuous outer triangular expanding focusing flow channel, the liquid inlet end of the continuous liquid phase flow channel is communicated with the liquid outlet end of the continuous outer triangular expanding focusing flow channel, the gas outlet end of the gas inlet flow channel is communicated with the continuous liquid phase flow channel, and the gas inlet flow channel is communicated with the gas inlet through the gas inlet end; the multistage heterogeneous reaction pool unit comprises a first-stage heterogeneous reaction pool unit and a second-stage heterogeneous reaction pool unit; the first-stage heterogeneous reaction cell unit comprises: the device comprises a first reaction liquid phase sample inlet, a first reaction liquid phase flow channel, a first heterogeneous reaction pool and a first liquid outlet flow channel; the liquid inlet end of the first reaction liquid phase flow channel is communicated with the first reaction liquid phase sample inlet, the liquid outlet end of the first reaction liquid phase flow channel is communicated with the liquid inlet end of the first heterogeneous reaction pool, the liquid outlet end of the continuous liquid phase flow channel is communicated with the liquid inlet end of the first heterogeneous reaction pool, and the liquid outlet end of the heterogeneous reaction pool is communicated with the liquid inlet end of the first liquid outlet flow channel; the second-stage heterogeneous reaction cell unit comprises: the device comprises a first reaction liquid phase sample inlet, a first reaction liquid phase flow channel, a first heterogeneous reaction pool, a first liquid outlet flow channel and a mixed phase sample outlet; the liquid inlet end of the second reaction liquid phase flow channel is communicated with a second reaction liquid phase sample inlet, the liquid outlet end of the second reaction liquid phase flow channel is communicated with the liquid inlet end of the second heterogeneous reaction pool, the liquid outlet end of the first liquid outlet flow channel is communicated with the liquid inlet end of the second heterogeneous reaction pool, the liquid outlet end of the heterogeneous reaction pool is communicated with the second liquid outlet flow channel, and the liquid outlet end of the second liquid outlet flow channel is communicated with a mixed phase sample outlet; the active valve quantitative uniform control unit comprises: the continuous liquid phase flow passage comprises a first active valve, a second active valve and a third active valve, wherein the first active valve is arranged in the continuous liquid phase flow passage, the second active valve is arranged in the first liquid outlet flow passage, and the third active valve is arranged in the second liquid outlet flow passage; the first active valve includes: a valve plug, a gas phase sample inlet, a gas phase channel and a gas buffer chamber are arranged in the gas buffer chamber; the built-in valve plug is arranged in the continuous liquid phase flow channel, the gas outlet end of the gas phase channel is communicated with the gas buffer chamber, the gas inlet end of the gas phase channel is communicated with the gas phase sample inlet, and the gas buffer chamber corresponds to the built-in valve plug; the second active valve and the third active valve are both the same in structure as the first active valve.

The multi-stage reaction micro-channel structure comprises a continuous outer triangular expansion focusing unit, an active valve quantitative uniform control unit and a multi-stage non-homogeneous reaction unit, wherein the continuous outer triangular expansion focusing unit comprises a continuous liquid phase injection port, a continuous outer triangular expansion focusing flow channel and a continuous liquid phase flow channel, the continuous liquid phase injection port is used for introducing samples (liquid drops or particles), the samples are separated layer by layer through the continuous outer triangular structure of the continuous outer triangular expansion focusing flow channel, so that the samples form microspheres which are same in size and distributed equidistantly and enter a continuous liquid phase channel, inert gas is introduced into the continuous liquid phase flow channel through the gas inlet channel, the distance of the microspheres is increased, the stability of the interval distribution of the microspheres is enhanced, the opening and closing degree of the continuous liquid phase channel is controlled through a first active valve of the active valve quantitative uniform control unit, and the flow rate of the microspheres is controlled, realizing quantitative control, entering a mixed liquid phase flow channel, leading in one of reaction liquid through a first reaction liquid phase sample inlet, leading the reaction liquid into a first heterogeneous reaction pool through a first reaction liquid phase flow channel to be collected and fully reacted, controlling the opening and closing degree of a first liquid outlet flow channel through a second active valve of an active valve quantitative uniform control unit, leading a sample in the first heterogeneous reaction pool and the reaction liquid to be fully reacted, ensuring the sufficiency of the reaction, discharging the reaction liquid from the first liquid outlet flow channel after the reaction is finished and leading the reaction liquid into a second heterogeneous reaction pool, leading in another reaction liquid through a human reaction liquid phase sample inlet, leading the reaction liquid into a second heterogeneous reaction pool to be collected and fully reacted, controlling the opening and closing degree of the first liquid outlet flow channel through the second active valve and controlling the opening and closing degree of the second liquid outlet flow channel through a third active valve, make the reaction liquid in the heterogeneous reaction tank of second fully react to realize realizing that quick accurate quantitative control carries out accurate high-efficient heterogeneous reaction, solved how to design a micro-fluidic device and operation technology, make it can be on the basis of generating high dispersion liquid drop, granule, realize that quick accurate quantitative control carries out accurate high-efficient heterogeneous reaction, and improve the technical problem of the sufficiency of reaction.

Drawings

In order to more clearly illustrate the detailed description of the present application or the technical solutions in the prior art, the drawings needed to be used in the detailed description of the present application or the prior art description will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a plan view of a multi-stage reaction microchannel structure according to an embodiment of the present invention;

FIG. 2 is a top view of a continuous outer triangular expanding focusing flow channel provided in an embodiment of the present application;

FIG. 3 is a control schematic diagram of an active valve quantitative uniformity control unit in an embodiment of the application;

FIG. 4 is a top view of a multi-stage heterogeneous reaction cell unit provided in an embodiment of the present application;

fig. 5 is an overall structural diagram of a microfluidic chip provided in an embodiment of the present application.

Wherein, the continuous outer triangle expansion focusing unit 1, the active valve quantitative uniform control unit 2, the first-stage heterogeneous reaction cell unit 3, the second-stage heterogeneous reaction cell unit 4, the continuous liquid phase sample inlet 5, the continuous outer triangle expansion focusing flow channel 6, the continuous liquid phase flow channel 7, the gas inlet 8, the gas inlet flow channel 9, the first reaction liquid phase sample inlet 10, the first reaction liquid phase flow channel 11, the first flow channel 110, the second flow channel 111, the first heterogeneous reaction cell 12, the first liquid outlet flow channel 13, the second reaction liquid phase sample inlet 14, the second reaction liquid phase flow channel 15, the third flow channel 150, the fourth flow channel 151, the second heterogeneous reaction cell 16, the second liquid outlet flow channel 17, the mixed phase sample outlet 18, the first active valve 19, the second active valve 20, the third active valve 21, the built-in valve plug 22, the gas phase sample inlet 23, the gas phase channel 24, the gas buffer chamber 25, the trapezoid valve block 26, A rectangular valve block 27, a base plate 28, a cover plate 29, a first transfer pump 30, a second transfer pump 31, a third transfer pump 32, a fourth transfer pump 33, a fifth transfer pump 34, a sixth transfer pump 35, a seventh transfer pump 36, and an extraction device 37.

Detailed Description

The technical solutions of the present application will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present application. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

Unless expressly stated or limited otherwise, the terms "mounted," "connected," and "connected" are intended to be inclusive and mean, for example, that they may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.

For easy understanding, please refer to fig. 1 to 4, fig. 1 is a top view of a multi-stage reaction microchannel structure provided in an embodiment of the present application; FIG. 2 is a top view of a continuous outer triangular expanding focusing flow channel provided in an embodiment of the present application; FIG. 3 is a control schematic diagram of an active valve quantitative uniformity control unit in an embodiment of the application; fig. 4 is a top view of a multi-stage heterogeneous reaction cell unit provided in an embodiment of the present application.

The embodiment of the application provides a multistage reaction microchannel structure, includes: the device comprises a continuous outer triangular expansion focusing unit 1, a driving valve quantitative uniform control unit 2 and a multi-stage heterogeneous reaction pool unit;

the continuous outer triangular expanding focusing unit 1 includes: the continuous liquid phase sampling device comprises a continuous liquid phase sampling port 5, a continuous outer triangular expansion focusing flow channel 6, a continuous liquid phase flow channel 7, an air inlet 8 and an air inlet flow channel 9;

the continuous liquid phase sample inlet 5 is communicated with the liquid inlet end of the continuous external triangular expanding and focusing flow channel 6, the liquid inlet end of the continuous liquid phase flow channel 7 is communicated with the liquid outlet end of the continuous external triangular expanding and focusing flow channel 6, the gas outlet end of the gas inlet flow channel 9 is communicated with the continuous liquid phase flow channel 7, and the gas inlet flow channel 9 is communicated with the gas inlet 8 through the gas inlet end;

the multi-stage heterogeneous reaction pool unit comprises a first-stage heterogeneous reaction pool unit 3 and a second-stage heterogeneous reaction pool unit 4;

the primary heterogeneous reaction cell unit 3 includes: a first reaction liquid phase sample inlet 10, a first reaction liquid phase flow channel 11, a first heterogeneous reaction pool 12 and a first liquid outlet flow channel 13;

the liquid inlet end of the first reaction liquid phase flow channel 11 is communicated with the first reaction liquid phase sample inlet 10, the liquid outlet end of the first reaction liquid phase flow channel is communicated with the liquid inlet end of the first heterogeneous reaction pool 12, the liquid outlet end of the continuous liquid phase flow channel 7 is communicated with the liquid inlet end of the first heterogeneous reaction pool 12, and the liquid outlet end of the heterogeneous reaction pool is communicated with the liquid inlet end of the first liquid outlet flow channel 13;

the secondary heterogeneous reaction cell unit 4 includes: a second reaction liquid phase sample inlet 14, a second reaction liquid phase flow channel 15, a second heterogeneous reaction pool 16, a second liquid outlet flow channel 17 and a mixed phase sample outlet 18;

the liquid inlet end of the second reaction liquid phase flow channel 15 is communicated with the second reaction liquid phase sample inlet 14, the liquid outlet end of the second reaction liquid phase flow channel is communicated with the liquid inlet end of the second heterogeneous reaction pool 16, the liquid outlet end of the first liquid outlet flow channel 13 is communicated with the liquid inlet end of the second heterogeneous reaction pool 16, the liquid outlet end of the heterogeneous reaction pool is communicated with the second liquid outlet flow channel 17, and the liquid outlet end of the second liquid outlet flow channel 17 is communicated with the mixed phase sample outlet 18;

the active valve quantitative uniformity control unit 2 includes: the continuous liquid phase flow passage comprises a first active valve 19, a second active valve 20 and a third active valve 21, wherein the first active valve 19 is arranged in the continuous liquid phase flow passage 7, the second active valve 20 is arranged in the first liquid outlet flow passage 13, and the third active valve 21 is arranged in the second liquid outlet flow passage 17;

the first active valve 19 includes: a built-in valve plug 22, a gas phase sample inlet 23, a gas phase channel 24 and a gas buffer chamber 25;

the built-in valve plug 22 is arranged in the continuous liquid phase flow passage 7, the gas outlet end of the gas phase channel 24 is communicated with the gas buffer chamber 25, the gas inlet end is communicated with the gas phase sample inlet 23, and the gas buffer chamber 25 corresponds to the built-in valve plug 22;

the second active valve 20 and the third active valve 21 are each identical in structure to the first active valve 19.

It should be noted that the inner side wall of the continuous outer triangular expanding focusing flow channel 6 is a continuous zigzag shape, the overlooking angle is similar to a plurality of continuous triangles, and the triangle is preferably an equilateral triangle, so as to realize that samples are equally dispersed to form more uniform microsphere particles, the narrowest part of the continuous outer triangular expanding focusing flow channel 6 is the same as the cross section of the continuous liquid phase flow channel 7, so that the continuous outer triangular expanding focusing flow channel can be just butted, the bottom surface of the continuous outer triangular expanding focusing flow channel 6 is preferably a smooth wall surface, so as to be beneficial to the flow of the samples, and the wall surface of the corresponding continuous liquid phase flow channel 7 can also be preferably. Preferably, in order to prevent the wall surface from being damaged due to the excessive pressure at the contact point of the wall surface of the continuous liquid phase flow channel 7 caused by the direct contact between the gas phase channel 24 and the wall surface of the continuous liquid phase flow channel 7, a gas buffer chamber 25 is provided to separate the gas phase channel 24 and the liquid phase flow channel wall, and the buffer chamber is kept at a certain distance from the wall surface of the liquid phase flow channel.

The first-stage heterogeneous reaction cell unit 3 comprises a first active valve 19, a first reaction liquid phase sample injection channel, a first reaction liquid phase sample injection port 10 and a first heterogeneous reaction cell 12, wherein the tail end of the high-dispersion liquid drop (or microsphere) continuous liquid phase flow channel 7 is quantitatively and uniformly controlled. Preferably, the first reaction liquid phase injection port 10 and the first reaction liquid phase flow channel 11 are both preferably two, and one first reaction liquid phase injection port 10 is matched with one first reaction liquid phase flow channel 11, so that simultaneous addition of different reaction liquids can be realized, and the functionality of the multi-stage reaction micro flow channel structure is stronger, preferably, the first reaction liquid phase flow channel 11 comprises a first flow channel 110 and a second flow channel 111, one end of the first flow channel 110 is communicated with one end of the second flow channel 111, the other end of the first flow channel 110 is communicated with the first reaction liquid phase injection port 10, the other end of the second flow channel 111 is communicated with a liquid inlet end of the first heterogeneous reaction pool 12, the first flow channel 110 and the second flow channel 111 are same-diameter pipes, the first flow channel 110 is in a horizontal direction, and the direction of the second flow channel 111 forms an included angle with the first flow channel 110. The caliber of the first liquid outlet flow channel 13 is larger than the calibers of the continuous liquid phase flow channel 7 and the first reaction liquid phase flow channel 11, so that the flow of the first liquid outlet flow channel 13 is larger.

Preferably, the second-stage heterogeneous reaction cell unit 4 includes a second active valve 20, a second reaction liquid phase sample injection channel, a second reaction liquid phase sample injection port 14, and a second heterogeneous reaction cell 16, wherein the second active valve 20 is used for quantitatively and uniformly controlling the tail end of the first liquid outlet channel 13 of the highly dispersed liquid droplets (or microspheres). Preferably, the second reaction liquid phase sample inlet 14 and the second reaction liquid phase flow channel 15 are both preferably two, and one second reaction liquid phase sample inlet 14 is matched with one second reaction liquid phase flow channel 15, so that simultaneous addition of different reaction liquids can be realized, and the functionality of the multi-stage reaction micro flow channel structure is stronger, preferably, the second reaction liquid phase flow channel 15 comprises a third flow channel 150 and a fourth flow channel 151, one end of the third flow channel 150 is communicated with one end of the fourth flow channel 151, the other end of the third flow channel 150 is communicated with the second reaction liquid phase sample inlet 14, the other end of the fourth flow channel 151 is communicated with a liquid inlet end of the second heterogeneous reaction pool 16, the third flow channel 150 and the fourth flow channel 151 are same-diameter pipes, the third flow channel 150 is in a horizontal direction, and the direction of the fourth flow channel 151 forms an included angle with the third flow channel 150. The aperture of the second liquid outlet channel 17 is larger than the aperture of the second liquid outlet channel 17 and the aperture of the second reaction liquid phase channel 15, so that the flow rate of the second liquid outlet channel 17 is larger.

The multistage reaction microchannel structure that this application embodiment provided, its multistage heterogeneous reaction pond unit can also include tertiary heterogeneous reaction pond unit, level four heterogeneous reaction pond unit etc. except including one-level heterogeneous reaction pond unit 3, second grade heterogeneous reaction pond unit 4, and specific progression setting can be selected according to actual conditions is nimble to reach purposes such as multistage reaction, multistage detection, multistep preparation. The multi-stage reaction tank can be applied to preparing samples by a step-by-step precipitation method or carrying out various step-by-step (stage) reactions.

Preferably, the structure of the second active valve 20 is the same as that of the first active valve 19, specifically, the built-in valve plug 22 of the second active valve 20 is disposed in the liquid outlet channel, the gas outlet end of the gas phase channel 24 of the second active valve 20 is communicated with the buffer chamber of the second active valve 20, the gas inlet end is communicated with the gas phase sample inlet 23 of the second active valve 20, and the gas buffer chamber 25 of the second active valve 20 corresponds to the built-in valve plug 22 of the second active valve 20, so as to realize the open and close control of the liquid outlet channel. The gas buffer chamber 25 of the first active valve 19 and the gas buffer chamber 25 of the second active valve 20 are preferably tapered gas buffer chambers 25, the bottoms of the tapered gas buffer chambers 25 correspond to the flow channels, and the distance between the tapered gas buffer chambers 25 and the wall of the liquid phase flow channel is preferably 30 to 100 μm. The structure of the third active valve 21 is also the same as that of the first active valve 19, and will not be described again.

The multi-stage reaction micro-channel structure comprises a continuous outer triangular expansion focusing unit 1, an active valve quantitative uniform control unit 2 and a multi-stage non-homogeneous reaction unit, wherein the continuous outer triangular expansion focusing unit 1 comprises a continuous liquid phase injection port 5, a continuous outer triangular expansion focusing flow channel 6 and a continuous liquid phase flow channel 7, the continuous liquid phase injection port 5 is used for introducing samples (liquid drops or particles), the samples are separated layer by layer through the continuous outer triangular structure of the continuous outer triangular expansion focusing flow channel 6, so that the samples form microspheres which are same in size and distributed equidistantly and enter a continuous liquid phase channel, an inert gas is introduced into the continuous liquid phase flow channel 7 through an air inlet channel 9, the distance of the microspheres is increased, the stability of the spaced distribution of the microspheres is enhanced, and the opening and closing degree of the continuous liquid phase channel is controlled through a first active valve 19 of the active valve quantitative uniform control unit 2, thereby controlling the flow of the microspheres, realizing quantitative control, entering a mixed liquid phase flow channel, introducing one of reaction liquids through a first reaction liquid phase sample inlet 10, and making the reaction liquid enter a first heterogeneous reaction tank 12 through a first reaction liquid phase flow channel 11 to be converged and fully reacted, controlling the opening and closing degree of a first liquid outlet flow channel 13 through a second active valve 20 of an active valve quantitative uniform control unit 2, so that a sample in the first heterogeneous reaction tank 12 and the reaction liquid can be fully reacted, ensuring the sufficiency of the reaction, after the reaction is finished, discharging the reaction liquid from the first liquid outlet flow channel 13 and introducing the reaction liquid into a second heterogeneous reaction tank 16, introducing another reaction liquid through a human reaction liquid phase sample inlet, making the reaction liquid enter the second heterogeneous reaction tank 16 to be converged and fully reacted, controlling the opening and closing degree of the first liquid outlet flow channel 13 through the second active valve 20, and controlling the opening and closing degree of a second liquid outlet flow channel 17 through a third active valve 21, make the reaction liquid in the heterogeneous reaction tank 16 of second fully react to realize realizing that quick accurate quantitative control carries out accurate high-efficient heterogeneous reaction, solved how to design a micro-fluidic device and operation technology, make it can be on the basis of generating high dispersion liquid drop, granule, realize that quick accurate quantitative control carries out accurate high-efficient heterogeneous reaction, and improve the technical problem of the sufficiency of reaction.

As a further improvement, the continuous outer triangular expanding focusing flow channel 6 of the multi-stage reaction micro-channel structure provided by the embodiment of the application is spiral;

the liquid inlet end of the continuous outer triangular expansion focusing flow channel 6 is positioned at the center of the spiral shape;

the liquid outlet end of the continuous outer triangular expansion focusing flow passage 6 is positioned at the outer side of the spiral shape.

Particularly, the spiral structure is favorable for reducing the maximum length of the continuous outer triangular expansion flow channel under the condition of occupying area as small as possible, so that the separation and dispersion effects on the sample are better, the introduced sample can be relatively disordered fluid such as focused liquid drops or liquid drops wrapping particles, after entering the continuous outer triangular expansion focusing flow channel, the disordered fluid containing the particles is acted by centrifugal force and dean flow force and flows along the inner wall surface of the triangle, and finally high dispersion and stable arrangement are realized, so that the focusing flow can be effectively increased, and the dispersion stability is improved.

As a further improvement, the active valve quantitative uniformity control unit 2 of the multi-stage reaction microchannel structure provided in the embodiment of the present application has at least one first active valve 19 and at least one second active valve 20. It is preferably two all, through setting up two first initiative valves 19, be favorable to it to carry out hierarchical control to the flow in the continuous liquid phase runner 7, makes the flow in the continuous liquid phase runner 7 reduce step by step to be favorable to controlling its final flow more accurately, specifically, two first initiative valves 19 parallel arrangement sets up from beginning to end, and the effect that sets up two second initiative valves 20 is the same with the effect that sets up two first initiative valves 19, and it is no longer repeated here.

As a further improvement, the present embodiment provides an internal valve plug 22 including a trapezoidal valve block 26 and a rectangular valve block 27;

the trapezoid valve block 26 is arranged on one side of the inner wall of the continuous liquid phase flow channel 7, which is far away from the gas buffer chamber 25, and the bottom surface of the trapezoid valve block 26 is attached to the inner wall of the continuous liquid phase flow channel 7;

the rectangular valve block 27 is arranged on one side of the inner wall of the continuous liquid phase flow channel 7 close to the gas buffer chamber 25, the rectangular valve block 27 and the trapezoidal valve block 26 are distributed in a staggered mode, and the side walls, corresponding to the rectangular valve block 27 and the trapezoidal valve block 26, of the continuous liquid phase flow channel 7 are located on the same cross section.

Specifically, when the gas buffer chamber 25 is inflated, deformed and expanded, the flow channel (which may be the continuous liquid phase flow channel 7, the first liquid outlet flow channel 13 or the second liquid outlet flow channel 17) is extruded through the bottom of the gas buffer chamber 25, so that the flow channel is deformed and drives the rectangular valve block 27 to move and approach the trapezoidal valve block 26, and thus the flow opening of the flow channel is gradually reduced, and thus the flow rate can be controlled, and when the rectangular valve block 27 and the trapezoidal valve block 26 are attached tightly, the flow channel is closed.

As a further improvement, the gas buffer chamber 25 and the continuous liquid phase flow channel 7 provided in the embodiment of the present application are both made of deformable materials, the gas buffer chamber 25 does not deform in a non-inflated state, the gas buffer chamber 25 expands in an inflated state and abuts against one side of the continuous liquid phase flow channel 7, and the inner wall of the continuous liquid phase flow channel 7 is in full contact with the built-in valve plug 22, so that the continuous liquid phase flow channel 7 is blocked. Specifically, the gas source in the gas phase channel 24 is gas introduced from the gas phase sample inlet 23, and the gas phase sample inlet 23 may be externally connected with a gas pump and other devices.

As a further improvement, the continuous liquid phase flow passage 7, the gas phase passage 24, the first reaction liquid phase flow passage 11, the second reaction liquid phase flow passage 15, and the first liquid outlet flow passage 13 and the second liquid outlet flow passage 17 provided in the embodiment of the present application are all rectangular in cross section, and the heights of the various flow passages are uniform.

Preferably, the total length of the continuous outer triangular expanding focusing flow channel 6 is 200 mm-2000 mm; the width of the continuous outer triangular expansion focusing flow channel 6 is 100-200 μm; the distance between two adjacent channels of the continuous outer triangular expanding focusing channel 6 is 200-400 μm; the curvature radius of the innermost flow passage of the continuous outer triangular expanding focusing flow passage 6 is 20 mm-60 mm.

Further, the side walls of the first heterogeneous reaction tank 12 and the second heterogeneous reaction tank 16 are both circular side walls, and the circular radiuses of the two are both 100um to 200 um.

Referring to fig. 1 to 5, the present application further provides a microfluidic chip, which includes a chip body and a multi-stage reaction microchannel structure in the above embodiment; the multi-stage reaction micro-channel structure is arranged in the chip body.

Optionally, the material of the chip body is preferably PDMS which is a transparent material, and the PDMS can be directly observed and photographed by using a microscope.

As a further improvement, the chip body of the microfluidic chip provided in the embodiment of the present application includes a substrate 28 and a cover plate 29; the multi-stage reaction micro-channel structure is arranged on the upper surface of the substrate 28; the cover plate 29 covers the upper surface of the substrate 28, and the continuous liquid phase sample inlet 5, the gas phase sample inlet 23, the reaction liquid phase sample inlet and the mixed phase sample outlet 18 are all communicated with the cover plate 29.

As a further improvement, the microfluidic chip provided in the embodiment of the present application further includes a conveying device and an extracting device 37; the conveying device comprises a first conveying pump 30 communicated with the continuous liquid phase sample inlet 5, a second conveying pump 31 communicated with the gas phase sample inlet 23 of the first active valve 19, a third conveying pump 32 communicated with the first reaction liquid phase sample inlet 10, a fourth conveying pump 33 communicated with the gas phase sample inlet 23 of the second active valve 20, a fifth conveying pump 34 communicated with the second reaction liquid phase sample inlet 14, a sixth conveying pump 35 communicated with the gas phase sample inlet 23 of the third active valve 21 and a seventh conveying pump 36 communicated with the gas inlet 8; the extraction device 37 is in communication with the mixed phase outlet 18. Wherein, the first delivery pump 30 is used for delivering the sample into the continuous liquid phase sample inlet 5; the seventh delivery pump 36 is used for delivering inert gas into the gas inlet flow channel 9, and then into the continuous liquid phase flow channel 7 through the gas inlet flow channel 9; the second delivery pump 31 is used for delivering gas into the gas phase sample inlet 23 of the first active valve 19; the third transfer pump 32 is configured to transfer a reaction liquid into the first reaction liquid phase sample inlet 10, the fourth transfer pump 33 is configured to transfer a gas into the gas phase sample inlet 23 of the second active valve 20, the fifth transfer pump 34 is configured to transfer another reaction liquid into the second reaction liquid phase sample inlet 14, and the sixth transfer pump 35 is configured to transfer a gas into the gas phase sample inlet 23 of the third active valve 21, specifically, the first reaction liquid phase sample inlet 10 and the first reaction liquid phase channel 11 are preferably two, and one first reaction liquid phase sample inlet 10 is matched with one first reaction liquid phase channel 11, so that the third transfer pumps 32 are preferably two, and each third transfer pump 32 can be respectively communicated with one first reaction liquid phase sample inlet 10 and is configured to transfer the same or different reaction liquids to the corresponding first reaction liquid phase sample inlets 10. The second reaction liquid phase injection port 14 and the second reaction liquid phase flow channel 15 are preferably two, and one second reaction liquid phase injection port 14 is matched with one second reaction liquid phase flow channel 15, so that the fifth transfer pumps 34 are preferably two, and each fifth transfer pump 34 can be respectively communicated with the second reaction liquid phase injection port 14 and is used for transferring the same or different reaction liquids to the corresponding second reaction liquid phase injection port 14. Preferably, since the first active valves 19 are preferably two, the number of the second delivery pumps 31 is also preferably two and is matched to the two first active valves 19, so that independent control of the two first active valves 19 is achieved. Since the second active valves 20 are preferably two, the number of the fourth delivery pumps 33 is also preferably two and is matched to the two second active valves 20, so that independent control of the two second active valves 20 is achieved.

The microfluidic chip is highly integrated, the whole chip area is small, and the chip area is only several cubic centimeters; the microfluidic chip has low cost and simple structure and is easy for batch production.

The micro-fluidic chip provided by the application has the following advantages:

1. the device structure is miniaturized, and has strong compatibility with other equipment. The whole microfluidic chip device is small in area but large in specific surface area, and high flux is achieved.

2. Can be widely applied to various heterogeneous reactions. A plurality of reaction units can be used simultaneously through a series or parallel micro-channel network, and the reaction units are isolated from each other, so that the reactions are not interfered with each other. The application field is wide. Because the liquid phase flow channel and the gas phase flow channel are not communicated, gas can not have any reaction on reaction liquid and particles, and the device is suitable for various heterogeneous reactions.

3. The device material can be replaced strongly. Such as glass chips, metal chips may be used as the chip material.

4. The controlled solid particles have high dispersion stability. The continuous external triangle expansion focusing flow channel is more beneficial to passive dean flow inertial focusing, effectively increases the focusing path and simultaneously regulates the stress of the inertial force, thereby improving the dispersibility and stability of the particles.

5. The materials in the reaction tank are accurately and quantitatively controlled. Thereby control solid particle quantity in the continuous liquid phase fast through adjusting pneumatic pump control toper gas buffer pressure size, realize accurate controllable quantitative control.

6. The reaction is uniform and sufficient. Through adjusting the opening and closing of the front and rear active control valves of the heterogeneous reaction tank, the heterogeneous reaction can be ensured to be uniformly and fully carried out in the reaction tank.

7. Environment-friendly and low in cost. The used chip material is non-toxic and harmless, and the reaction effect which is difficult to achieve by conventional operation can be achieved only by less particles and reaction liquid in the operation process.

8. Easy to observe. The device can select PDMS of transparent material for use as the chip material, can directly use the microscope to observe, take a picture the record.

9. Safe and reliable, and high-speed and high-efficiency reaction.

The present application also provides a heterogeneous reaction method applied to the multi-stage reaction microchannel structure according to any one of claims 1 to 6, comprising the steps of:

s1, uniformly and stably dispersing the microsphere suspension liquid through the continuous outer triangular expansion focusing flow channel, flowing into the continuous liquid phase channel, and introducing into the first heterogeneous reaction tank through the continuous liquid phase channel;

s2, adjusting the flow of the continuous liquid phase channel through a first active valve of the active valve quantitative and uniform control unit, and accurately controlling the quantity of microspheres in the microsphere suspension flowing into the first heterogeneous reaction tank;

s3, enabling a reaction liquid to enter a first heterogeneous reaction tank through a first reaction liquid phase flow channel, enabling the reaction liquid to be in short contact with a first heterogeneous microsphere suspension liquid, enabling the reaction liquid to enter the first heterogeneous reaction tank for reaction, and transmitting the reaction liquid to a second heterogeneous reaction tank through a first liquid outlet flow channel;

s4, adjusting the flow of the first liquid outlet flow channel through a second active valve of the active valve quantitative uniform control unit;

s5, enabling the other reaction liquid to enter a second heterogeneous reaction tank through a second reaction liquid phase flow channel, enabling the other reaction liquid to fully react with substances in the second heterogeneous reaction tank, and discharging the reacted substances from a second liquid outlet flow channel;

and S6, regulating the flow of the second liquid outlet channel through a third active valve of the active valve quantitative and uniform control unit.

The above is the first embodiment provided by the present application, and the following is the second embodiment provided by the present application, specifically:

the chip body is made of PDMS (polydimethylsiloxane), wherein the length of the continuous outer triangular expansion focusing flow channel is 1200mm, the distance between two adjacent vortex focusing flow channels is 100 micrometers, the curvature radius of the innermost flow channel is 40mm, the widths of the gas phase flow channel, the first reaction liquid phase flow channel, the continuous liquid phase flow channel and the second reaction liquid flow channel are 100 micrometers, the widths of the first liquid outlet flow channel and the second liquid outlet flow channel are 100 micrometers, the distance between the gas buffer chamber and the continuous liquid phase flow channel is 60 micrometers when the gas buffer chamber is in a non-working state, and the heights of all the flow channels are 150 micrometers. Selecting nitrogen as a gas phase, adding carbon spheres with a proper amount of particle size of 50 mu m into a mixed aqueous solution of magnesium nitrate and calcium nitrate to form a microsphere suspension, and using an ammonium carbonate aqueous solution and a sodium hydroxide aqueous solution as reaction liquids in a first-stage heterogeneous reaction tank unit and a second-stage heterogeneous reaction tank unit respectively. The microsphere suspension and the reaction solution were injected into the chip using teflon capillary tubes, respectively, and the gas phase fluid was controlled using a pneumatic pump. The flow rate of the microsphere suspension is 50 mul/min, the flow rate of the gas phase is 80 mul/min, and the flow rates of the reaction liquid in the first reaction liquid phase flow channel, the first liquid outlet flow channel and the second reaction liquid phase flow channel are all 40 mul/min. The quantitative carbon spheres in the microsphere suspension and the ammonium carbonate aqueous solution enter the first heterogeneous reaction tank together by adjusting the flow of the gas phase and the microsphere suspension, all the active valves at the front and the back of the reaction tank are closed, so that the ammonium carbonate solution and the mixed aqueous solution of cobalt nitrate and calcium nitrate with the carbon spheres carry out accurate, efficient and sufficient heterogeneous reaction, and the calcium hydroxide precipitates are uniformly attached to the surface of the carbon sphere template. Opening a front driving valve and a rear driving valve of the first heterogeneous reaction tank, adjusting the flow of gas phase and calcium hydroxide/carbon sphere suspension liquid, controlling the quantity of particles, simultaneously introducing sodium hydroxide aqueous solution into a third flow channel and a fourth flow channel, enabling the sodium hydroxide aqueous solution and the sodium hydroxide aqueous solution to jointly enter a second heterogeneous reaction tank, closing the front driving valve and the rear driving valve of the second heterogeneous reaction tank, and carrying out uniform and sufficient secondary reaction in the tanks to generate (calcium hydroxide-cobalt hydroxide)/carbon spheres. And the obtained substance is led out from the third mixed phase sample outlet.

The above is the second embodiment provided by the present application, and the following is the third embodiment provided by the present application, specifically:

the chip body is made of PDMS, wherein the length of the continuous outer triangular expansion focusing flow channel is 1600mm, the distance between two adjacent vortex focusing flow channels is 80 μm, the curvature radius of the innermost flow channel is 50mm, the widths of the gas phase flow channel, the first reaction liquid phase flow channel, the continuous liquid phase flow channel and the second reaction liquid flow channel are 120 μm, the widths of the mixed liquid phase flow channel and the liquid outlet flow channel are 160 μm, the gas buffer chamber keeps a certain distance with the wall of the liquid phase flow channel in a non-working state, the distance is 80 μm, and the heights of all the flow channels are 200 μm. Selecting nitrogen as a gas phase, adding carbon spheres with a proper amount of particle size of 50 mu m into a mixed aqueous solution of magnesium nitrate and calcium nitrate to form a microsphere suspension, and using an ammonium carbonate aqueous solution and a sodium hydroxide aqueous solution as reaction liquids in a first-stage heterogeneous reaction tank unit and a second-stage heterogeneous reaction tank unit respectively. The microsphere suspension and the reaction solution were injected into the chip using teflon capillary tubes, respectively, and the gas phase fluid was controlled using a pneumatic pump. The flow rate of the microsphere suspension is 60 mul/min, the gas phase flow rate is 80 mul/min, and the flow rates of the reaction liquid in the first reaction liquid phase flow channel, the first liquid outlet flow channel and the second reaction liquid phase flow channel are all 50 mul/min. The quantitative carbon spheres in the microsphere suspension and the ammonium carbonate aqueous solution enter the first heterogeneous reaction tank together by adjusting the flow of the gas phase and the microsphere suspension, all the active valves at the front and the back of the reaction tank are closed, so that the ammonium carbonate solution and the mixed aqueous solution of cobalt nitrate and calcium nitrate with the carbon spheres carry out accurate, efficient and sufficient heterogeneous reaction, and the calcium hydroxide precipitates are uniformly attached to the surface of the carbon sphere template. Opening front and rear active valves of the first reaction tank unit, adjusting the flow of gas phase and calcium hydroxide/carbon sphere suspension liquid, controlling the particle quantity, simultaneously introducing sodium hydroxide aqueous solution into the third flow channel and the fourth liquid phase flow channel, enabling the sodium hydroxide aqueous solution and the sodium hydroxide aqueous solution to jointly enter a second heterogeneous reaction tank, closing the front and rear active valves of the second heterogeneous reaction tank, and carrying out uniform and sufficient secondary reaction in the second heterogeneous reaction tank to generate (calcium hydroxide-magnesium hydroxide)/carbon spheres. And then the obtained substance is led out from the mixed phase sample outlet.

The above embodiments are only used for illustrating the technical solutions of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present application.