阅读说明：本技术 一种微流道结构、微流控芯片以及非均相反应方法 (Micro-channel structure, micro-fluidic chip and heterogeneous reaction method ) 是由 王超 蒋志强 于 2021-01-13 设计创作，主要内容包括：本申请提供了一种微流道结构、微流控芯片和非均相反应方法,微流道结构包括：连续外三角扩张聚焦单元、主动阀定量均匀控制单元和同轴流非匀相反应单元；连续外三角扩张聚焦单元包括：连续液相进样口、连续外三角扩张聚焦流道和连续液相流道；主动阀定量均匀控制单元包括：流道内置阀塞、气相进样口、气相通道和气体缓冲室；连续液相流道内壁设有内置阀塞；同轴流非匀相反应单元包括：反应液相进样口、反应液相流道和混合液相流道。本申请解决了如何设计一种微流控装置和操作工艺,使其能够在生成高分散液滴、颗粒的基础上,实现快速准确的定量控制进行精确高效非均相反应的技术问题。(The application provides a micro-channel structure, a micro-fluidic chip and a heterogeneous reaction method, wherein the micro-channel structure comprises: the device comprises a continuous outer triangular expansion focusing unit, an active valve quantitative uniform control unit and a coaxial flow heterogeneous reaction unit; the continuous outer triangular expanding focusing unit comprises: the continuous liquid phase sampling port, the continuous outer triangular expansion focusing flow channel and the continuous liquid phase flow channel; the active valve quantitative uniform control unit comprises: a valve plug, a gas phase sample inlet, a gas phase channel and a gas buffer chamber are arranged in the flow channel; the inner wall of the continuous liquid phase flow passage is provided with a built-in valve plug; the coaxial flow heterogeneous reaction unit comprises: a reaction liquid phase sample inlet, a reaction liquid phase flow passage and a mixed liquid phase flow passage. The application solves the technical problem of how to design a microfluidic device and an operation process, so that the microfluidic device can realize rapid and accurate quantitative control to carry out accurate and efficient heterogeneous reaction on the basis of generating high-dispersion liquid drops and particles.)

1. A micro flow channel structure, comprising: the device comprises a continuous outer triangular expansion focusing unit, an active valve quantitative uniform control unit and a coaxial flow heterogeneous reaction unit;

the continuous outer triangular expanding focusing unit includes: the continuous liquid phase sampling port, the continuous outer triangular expansion focusing flow channel and the continuous liquid phase flow channel;

the continuous liquid phase sample inlet is communicated with the liquid inlet end of the continuous outer triangular expansion focusing flow channel, and the liquid inlet end of the continuous liquid phase flow channel is communicated with the liquid outlet end of the continuous outer triangular expansion focusing flow channel;

the active valve quantitative uniform control unit comprises: a valve plug, a gas phase sample inlet, a gas phase channel and a gas buffer chamber are arranged in the gas buffer chamber;

the inner wall of the continuous liquid phase flow channel is provided with the built-in valve plug, the gas outlet end of the gas phase channel is communicated with the gas buffer chamber, the gas inlet end of the gas phase channel is communicated with the gas phase sample inlet, and the gas buffer chamber corresponds to the built-in valve plug;

the coaxial flow heterogeneous reaction unit comprises: a reaction liquid phase sample inlet, a reaction liquid phase flow channel and a mixed liquid phase flow channel;

the liquid inlet end of the reaction liquid phase flow channel is communicated with the reaction liquid phase sample inlet, the liquid outlet end of the reaction liquid phase flow channel is communicated with the liquid inlet end of the mixed liquid phase flow channel, and the liquid outlet end of the mixed liquid phase flow channel is communicated with the mixed phase sample outlet; and the liquid inlet end of the mixed liquid phase flow channel is communicated with the continuous liquid phase flow channel.

2. The micro flow channel structure of claim 1, wherein the continuous outer triangularly expanded focusing flow channel is in a spiral shape;

the liquid inlet end of the continuous outer triangular expanding focusing flow channel is positioned at the center of the spiral shape;

the liquid outlet end of the continuous outer triangular expansion focusing flow passage is positioned at the outer side of the spiral shape.

3. The micro flow channel structure of claim 1, wherein at least one of the active valve quantitative uniformity control units.

4. The micro flow channel structure of claim 1, wherein the built-in valve plug is in a block shape;

the built-in valve plug is arranged on one side, far away from the gas buffer chamber, of the inner wall of the continuous liquid phase flow channel.

5. The micro flow channel structure of claim 4, wherein the gas buffer chamber and the continuous liquid phase flow channel are both made of deformable materials, the gas buffer chamber is not deformed in a non-inflated state, the gas buffer chamber is expanded in an inflated state and abuts against one side of the continuous liquid phase flow channel, and the inner wall of the continuous liquid phase flow channel is in full contact with the built-in valve plug, so that the continuous liquid phase flow channel is blocked.

6. The micro flow channel structure of claim 1, wherein the continuous liquid phase flow channel, the gas phase channel and the reaction liquid phase flow channel are rectangular in cross section, and the height of each flow channel is uniform and is 100 μm to 200 μm.

7. A microfluidic chip comprising a chip body and the micro flow channel structure of any of claims 1 to 6;

the micro-channel structure is arranged in the chip body.

8. The microfluidic chip according to claim 7, wherein the chip body comprises a substrate and a cover plate;

the micro-channel structure is arranged on the upper surface of the substrate;

the apron cover in the upper surface of base plate, just continuous liquid phase introduction port the gas phase introduction port reaction liquid phase introduction port with mixed phase appearance mouth all link up in the apron.

9. The microfluidic chip according to claim 7, further comprising a delivery device and an extraction device;

the conveying device comprises a first conveying pump communicated with the continuous liquid phase sample inlet, a second conveying pump communicated with the gas phase sample inlet, and a third conveying pump communicated with the reaction liquid phase sample inlet;

the extraction device is communicated with the mixed phase sample outlet.

10. A heterogeneous reaction method applied to the micro flow channel structure according to any one of claims 1 to 6, comprising the steps of:

uniformly and stably dispersing the microsphere suspension liquid through a continuous external triangular expansion focusing flow channel and flowing the microsphere suspension liquid into a continuous liquid phase channel;

the opening and closing of the continuous liquid phase channel are adjusted through a gas phase channel of the active valve quantitative uniform control unit;

and (3) enabling the reaction liquid to enter the mixed liquid phase flow channel through the reaction liquid phase flow channel, and mixing and reacting with the microsphere suspension in the mixed liquid phase flow channel.

Technical Field

The application relates to the technical field of microfluidics, in particular to a micro-channel structure, a micro-fluidic chip and a heterogeneous reaction method.

Background

With the development of science and technology, more and more fields (energy, immunity, biochemistry, and the like) need to use a miniaturized reaction means to perform highly dispersed micro precise operations, and the micro-fluidic technology has attracted extensive attention because of being capable of realizing a lot of micro-processing and micro-operation which are difficult to be completed. Microfluidics is a manipulation of minute particles (or samples) that cannot be achieved by some conventional methods using microchannels and devices. The method can integrate biological detection, a series of biochemical reactions and preparation of various samples on a tiny chip for special operation, and has wide application prospect in multiple fields.

At present, the conventional preparation process of liquid drops or microsphere particles mainly adopts a large-scale mechanical stirring method, so that the microsphere particles with specific particle size cannot be accurately screened, the particle dispersibility is low, and the high-efficiency reaction cannot be ensured when the number of liquid drops (or particles) participating in the reaction is too large or too small. The liquid drops (or particles) can be uniformly dispersed by a micro-fluidic system with a special structure, effective and sufficient reaction is carried out after quantitative control, and the efficiency and the success rate of experiments can be effectively improved.

There are many methods of generating (or wrapping droplets of particles) using microfluidics, which actively require an external magnetic field and an electric field; the passive mode usually adopts dean flow, does not need energy input, and the device is simple and convenient, easy to maintain and small in size. Passive dean flow is currently one of the most effective means for microfluidic focusing of droplets (or particle-encapsulated droplets) due to its simplicity and convenience of operation, uniformity and high efficiency. By passive dean flow focusing, the micro-ball and liquid drop scattered in disorder can be focused to form micro-ball and liquid drop queue arranged at equal intervals at specific positions in the micro-channel. Although the dispersion of the liquid drops (or particles) is achieved to a certain extent, a spiral bent flow channel with a certain length is required to achieve the purpose, and accurate quantitative control is difficult to perform.

Therefore, how to design a microfluidic device and an operation process to enable the microfluidic device to realize rapid and accurate quantitative control and perform accurate and efficient heterogeneous reaction on the basis of generating highly dispersed droplets and particles becomes one of the problems to be solved by the technology in the field.

Disclosure of Invention

The application aims to provide a micro-channel structure, a micro-fluidic chip and a heterogeneous reaction method, which are used for solving the technical problem of how to design a micro-fluidic device and an operation process so that the micro-fluidic device can realize rapid and accurate quantitative control to carry out accurate and efficient heterogeneous reaction on the basis of generating highly dispersed liquid drops and particles.

In order to solve the above problems, the present application provides a micro flow channel structure comprising: the device comprises a continuous outer triangular expansion focusing unit, an active valve quantitative uniform control unit and a coaxial flow heterogeneous reaction unit;

the continuous outer triangular expanding focusing unit includes: the continuous liquid phase sampling port, the continuous outer triangular expansion focusing flow channel and the continuous liquid phase flow channel;

the continuous liquid phase sample inlet is communicated with the liquid inlet end of the continuous outer triangular expansion focusing flow channel, and the liquid inlet end of the continuous liquid phase flow channel is communicated with the liquid outlet end of the continuous outer triangular expansion focusing flow channel;

the active valve quantitative uniform control unit comprises: a valve plug, a gas phase sample inlet, a gas phase channel and a gas buffer chamber are arranged in the flow channel;

the inner wall of the continuous liquid phase flow channel is provided with the built-in valve plug, the gas outlet end of the gas phase channel is communicated with the gas buffer chamber, the gas inlet end of the gas phase channel is communicated with the gas phase sample inlet, and the gas buffer chamber corresponds to the built-in valve plug;

the coaxial flow heterogeneous reaction unit comprises: a reaction liquid phase sample inlet, a reaction liquid phase flow channel and a mixed liquid phase flow channel;

the liquid inlet end of the reaction liquid phase flow channel is communicated with the reaction liquid phase sample inlet, the liquid outlet end of the reaction liquid phase flow channel is communicated with the liquid inlet end of the mixed liquid phase flow channel, and the liquid outlet end of the mixed liquid phase flow channel is communicated with the mixed phase sample outlet; and the liquid inlet end of the mixed liquid phase flow channel is communicated with the continuous liquid phase flow channel.

Furthermore, the continuous outer triangular expanding focusing flow channel is spiral;

the liquid inlet end of the continuous outer triangular expanding focusing flow channel is positioned at the center of the spiral shape;

the liquid outlet end of the continuous outer triangular expansion focusing flow passage is positioned at the outer side of the spiral shape.

Furthermore, at least one active valve quantitative uniform control unit is arranged.

Furthermore, the built-in valve plug is in a block shape;

the built-in valve plug is arranged on one side, far away from the gas buffer chamber, of the inner wall of the continuous liquid phase flow channel.

Furthermore, the gas buffer chamber and the continuous liquid phase flow channel are both made of deformable materials, the gas buffer chamber does not deform in a non-inflation state, the gas buffer chamber expands in an inflation state and is abutted against one side of the continuous liquid phase flow channel, and the inner wall of the continuous liquid phase flow channel is in full contact with the built-in valve plug, so that the continuous liquid phase flow channel is blocked.

Furthermore, the cross sections of the continuous liquid phase flow channel, the gas phase channel and the reaction liquid phase flow channel are rectangular, the heights of the various flow channels are uniform, and the heights of the various flow channels are all 100-200 mu m.

The application also provides a micro-fluidic chip which comprises a chip body and the micro-channel structure;

the micro-channel structure is arranged in the chip body.

Further, the chip body comprises a substrate and a cover plate;

the micro-channel structure is arranged on the upper surface of the substrate;

the apron cover in the upper surface of base plate, just continuous liquid phase introduction port the gas phase introduction port reaction liquid phase introduction port with mixed phase appearance mouth all link up in the apron.

Further, the device also comprises a conveying device and an extracting device;

the conveying device comprises a first conveying pump communicated with the continuous liquid phase sample inlet, a second conveying pump communicated with the gas phase sample inlet, and a third conveying pump communicated with the reaction liquid phase sample inlet;

the extraction device is communicated with the mixed phase sample outlet.

The application also provides a heterogeneous reaction method applied to the micro-channel structure, which comprises the following steps:

uniformly and stably dispersing the microsphere suspension liquid through a continuous external triangular expansion focusing flow channel and flowing the microsphere suspension liquid into a continuous liquid phase channel;

the opening and closing of the continuous liquid phase channel are adjusted through a gas phase channel of the active valve quantitative uniform control unit;

and (3) enabling the reaction liquid to enter the mixed liquid phase flow channel through the reaction liquid phase flow channel, and mixing and reacting with the microsphere suspension in the mixed liquid phase flow channel.

Compared with the prior art, the embodiment of the application has the advantages that:

the application provides a micro flow channel structure, includes: the device comprises a continuous outer triangular expansion focusing unit, an active valve quantitative uniform control unit and a coaxial flow heterogeneous reaction unit; the continuous outer triangular expanding focusing unit includes: the continuous liquid phase sampling port, the continuous outer triangular expansion focusing flow channel and the continuous liquid phase flow channel; the continuous liquid phase sample inlet is communicated with the liquid inlet end of the continuous outer triangular expansion focusing flow channel, and the liquid inlet end of the continuous liquid phase flow channel is communicated with the liquid outlet end of the continuous outer triangular expansion focusing flow channel; the active valve quantitative uniform control unit comprises: a valve plug, a gas phase sample inlet, a gas phase channel and a gas buffer chamber are arranged in the flow channel; the inner wall of the continuous liquid phase flow channel is provided with the built-in valve plug, the gas outlet end of the gas phase channel is communicated with the gas buffer chamber, the gas inlet end of the gas phase channel is communicated with the gas phase sample inlet, and the gas buffer chamber corresponds to the built-in valve plug; the coaxial flow heterogeneous reaction unit comprises: a reaction liquid phase sample inlet, a reaction liquid phase flow channel and a mixed liquid phase flow channel; the liquid inlet end of the reaction liquid phase flow channel is communicated with the reaction liquid phase sample inlet, the liquid outlet end of the reaction liquid phase flow channel is communicated with the liquid inlet end of the mixed liquid phase flow channel, and the liquid outlet end of the mixed liquid phase flow channel is communicated with the mixed phase sample outlet; and the liquid inlet end of the mixed liquid phase flow channel is communicated with the continuous liquid phase flow channel.

The micro-channel structure comprises a continuous outer triangular expansion focusing unit, an active valve quantitative uniform control unit and a coaxial flow non-homogeneous reaction unit, wherein the continuous outer triangular expansion focusing unit comprises a continuous liquid phase injection port, a continuous outer triangular expansion focusing flow channel and a continuous liquid phase flow channel, the continuous liquid phase injection port is used for introducing samples (liquid drops or particles), the samples are separated layer by layer through the continuous outer triangular structure of the continuous outer triangular expansion focusing flow channel, so that the samples form microspheres which are same in size and distributed equidistantly and enter a continuous liquid phase channel, the opening and closing degree of the continuous liquid phase channel is controlled through the active valve quantitative uniform control unit, the flow of the microspheres is controlled, the quantitative control is realized, the microspheres finally enter a mixed liquid phase flow channel, reaction liquid is introduced into the reaction liquid phase injection port and enters the mixed liquid phase flow channel through the reaction liquid phase flow channel to be fully mixed with the microspheres, therefore, the method realizes rapid and accurate quantitative control to carry out accurate and efficient heterogeneous reaction, and solves the technical problem of how to design a micro-fluidic device and an operation process to realize rapid and accurate quantitative control to carry out accurate and efficient heterogeneous reaction on the basis of generating highly dispersed liquid drops and particles.

Drawings

In order to more clearly illustrate the detailed description of the present application or the technical solutions in the prior art, the drawings needed to be used in the detailed description of the present application or the prior art description will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a plan view of a micro flow channel structure according to an embodiment of the present invention;

FIG. 2 is a top view of a continuous outer triangular expanding focusing flow channel provided in an embodiment of the present application;

FIG. 3 is a control schematic diagram of an active valve quantitative uniformity control unit in an embodiment of the application;

fig. 4 is an overall structural diagram of a microfluidic chip provided in an embodiment of the present application.

Wherein the reference numerals are: the device comprises a continuous outer triangular expansion focusing unit 1, an active valve quantitative uniform control unit 2, a coaxial flow heterogeneous reaction unit 3, a continuous liquid phase sample inlet 4, a continuous outer triangular expansion focusing flow channel 5, a continuous liquid phase flow channel 6, a built-in plug valve 7, a gas phase sample inlet 8, a gas phase channel 9, a gas buffer chamber 10, a reaction liquid phase sample inlet 11, a reaction liquid phase flow channel 12, a mixed liquid phase flow channel 13, a substrate 14, a cover plate 15, a first delivery pump 16, a second delivery pump 17, a third delivery pump 18, an extraction device 19, a first flow channel 20, a second flow channel 21 and a mixed phase sample outlet 22.

Detailed Description

The technical solutions of the present application will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present application. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

Unless expressly stated or limited otherwise, the terms "mounted," "connected," and "connected" are intended to be inclusive and mean, for example, that they may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.

For ease of understanding, please refer to fig. 1-3.

The embodiment of the application provides a micro flow channel structure, its characterized in that includes: the device comprises a continuous outer triangular expansion focusing unit 1, an active valve quantitative uniform control unit 2 and a coaxial flow heterogeneous reaction unit;

the continuous outer triangular expanding focusing unit 1 includes: a continuous liquid phase sample inlet 4, a continuous outer triangular expansion focusing flow channel 5 and a continuous liquid phase flow channel 6;

the continuous liquid phase sample inlet 4 is communicated with the liquid inlet end of the continuous external triangular expanding focusing flow channel 5, and the liquid inlet end of the continuous liquid phase flow channel 6 is communicated with the liquid outlet end of the continuous external triangular expanding focusing flow channel 5;

the active valve quantitative uniformity control unit 2 includes: a valve plug 7, a gas phase sample inlet 8, a gas phase channel 9 and a gas buffer chamber 10 are arranged in the flow channel;

the inner wall of the continuous liquid phase flow passage 6 is provided with a built-in valve plug 7, the gas outlet end of the gas phase passage 9 is communicated with a gas buffer chamber 10, the gas inlet end is communicated with a gas phase sample inlet 8, and the gas buffer chamber 10 corresponds to the built-in valve plug;

the coaxial flow heterogeneous reaction unit comprises: a reaction liquid phase sample inlet 11, a reaction liquid phase flow channel 12 and a mixed liquid phase flow channel 13;

the liquid inlet end of the reaction liquid phase flow passage 12 is communicated with the reaction liquid phase sample inlet 11, the liquid outlet end is communicated with the liquid inlet end of the mixed liquid phase flow passage 13, and the liquid outlet end of the mixed liquid phase flow passage 13 is communicated with the mixed phase sample outlet 22; the liquid inlet end of the mixed liquid phase flow passage 13 is communicated with the continuous liquid phase flow passage 6.

It should be noted that the inner side wall of the continuous outer triangular expanding focusing flow channel 5 is a continuous zigzag shape, the overlooking angle is similar to a plurality of continuous triangles, and the triangle is preferably an equilateral triangle, so as to realize that samples are equally dispersed to form more uniform microsphere particles, the narrowest part of the continuous outer triangular expanding focusing flow channel 5 is the same as the cross section of the continuous liquid phase flow channel 6, so that the continuous outer triangular expanding focusing flow channel can be just butted, the bottom surface of the continuous outer triangular expanding focusing flow channel 5 is preferably a smooth wall surface, so as to be beneficial to the flow of the samples, and the wall surface of the corresponding continuous liquid phase flow channel 6 can also be preferably.

Preferably, in order to avoid the damage to the wall surface caused by the excessive pressure at the contact point of the wall surface of the continuous liquid phase flow channel 6 due to the direct contact between the gas phase channel 9 and the wall surface of the continuous liquid phase flow channel 6, a gas buffer chamber 10 is arranged to separate the gas phase channel 9 and the liquid phase flow channel wall, and the buffer chamber is kept at a certain distance from the wall surface of the liquid phase flow channel.

The coaxial flow heterogeneous reaction unit 3 consists of a highly dispersed liquid drop (or microsphere) continuous liquid phase flow channel 6 tail end, a reaction liquid phase sample injection flow channel and a mixed liquid phase flow channel 13 which are quantitatively and uniformly controlled by an active valve. Preferably, the reaction liquid phase injection port 11 and the reaction liquid phase flow channel 12 are both preferably two, and one reaction liquid phase injection port 11 is matched with one reaction liquid phase flow channel 12, so that simultaneous addition of two reaction liquids can be realized, and the micro flow channel structure has stronger functionality, and preferably, the reaction liquid phase flow channel 12 comprises a first flow channel 20 and a second flow channel 21, one end of the first flow channel 20 is communicated with one end of the second flow channel 21, the other end of the first flow channel 20 is communicated with the reaction liquid phase injection port 11, the other end of the second flow channel 21 is communicated with a liquid inlet end of the mixed liquid phase flow channel 13, the first flow channel 20 and the second flow channel 21 are same-diameter pipes, the first flow channel 20 is in a horizontal direction, and the direction of the second flow channel 21 and the first flow channel 20 form an included angle of 45 degrees. The aperture of the mixed liquid phase flow channel 13 is larger than the apertures of the continuous liquid phase flow channel 6 and the reaction liquid phase flow channel 12, so that the mixed liquid phase flow channel 13 can better accommodate the microspheres and various reaction liquids simultaneously.

The micro flow channel structure provided in the application comprises a continuous outer triangular expansion focusing unit 1, an active valve quantitative uniform control unit 2 and a coaxial flow heterogeneous reaction unit, wherein the continuous outer triangular expansion focusing unit 1 comprises a continuous liquid phase sample inlet 4, a continuous outer triangular expansion focusing flow channel 5 and a continuous liquid phase flow channel 6, the continuous liquid phase sample inlet 4 is used for introducing samples (liquid drops or particles), the samples are separated layer by layer through the continuous outer triangular structure of the continuous outer triangular expansion focusing flow channel 5, so that the samples form microspheres which are same in size and distributed equidistantly and enter a continuous liquid phase channel, the active valve quantitative uniform control unit 2 is used for controlling the opening and closing degree of the continuous liquid phase channel, thereby controlling the flow of the microspheres, realizing quantitative control, finally entering a mixed liquid phase flow channel 13, introducing a reaction liquid through a reaction liquid phase sample inlet 11, and enabling the reaction liquid to enter the mixed liquid phase flow channel 13 through a reaction liquid phase flow channel 12 to be fully mixed with the microspheres, therefore, the method realizes rapid and accurate quantitative control to carry out accurate and efficient heterogeneous reaction, and solves the technical problem of how to design a micro-fluidic device and an operation process to realize rapid and accurate quantitative control to carry out accurate and efficient heterogeneous reaction on the basis of generating highly dispersed liquid drops and particles.

As a further improvement, the continuous outer triangular expanding focusing flow channel 5 of the micro flow channel structure provided by the embodiment of the application is spiral;

the liquid inlet end of the continuous outer triangular expansion focusing flow channel 5 is positioned at the center of the spiral shape;

the liquid outlet end of the continuous outer triangular expansion focusing flow passage 5 is positioned at the outer side of the spiral shape.

Specifically, the spiral structure is beneficial to reducing the maximum length of the continuous outer triangular expansion flow channel under the condition of occupying the area as small as possible, so that the separation and dispersion effects on the sample are better.

As a further improvement, the active valve quantitative uniform control unit 2 of the micro flow channel structure provided by the embodiment of the application is at least one. Preferably, two active emission quantity uniform control units are arranged, so that the flow in the continuous liquid phase flow channel 6 can be controlled in a grading manner, the flow in the continuous liquid phase flow channel 6 is reduced step by step, and the final flow can be controlled more accurately.

As a further improvement, the built-in valve plug 7 provided in the embodiment of the present application is block-shaped;

the built-in valve plug 7 is arranged on one side of the inner wall of the continuous liquid phase flow passage 6 far away from the gas buffer chamber 10. Preferably, the internal valve plug 7 has a rectangular parallelepiped shape.

As a further improvement, the gas buffer chamber 10 and the continuous liquid phase flow channel 6 provided in the embodiment of the present application are both made of deformable materials, the gas buffer chamber 10 does not deform in a non-inflated state, the gas buffer chamber 10 expands in an inflated state and abuts against one side of the continuous liquid phase flow channel 6, and the inner wall of the continuous liquid phase flow channel 6 is in full contact with the built-in valve plug 7, so that the continuous liquid phase flow channel 6 is blocked. Specifically, the gas source in the gas phase channel 9 is gas introduced from the gas phase sample inlet 8, and the gas phase sample inlet 8 can be externally connected with devices such as a gas pump.

As a further improvement, the continuous liquid phase flow channel 6, the gas phase channel 9 and the reaction liquid phase flow channel 12 provided in the embodiments of the present application are rectangular in cross section, and the heights of the various flow channels are uniform and are all 100 μm to 200 μm.

Preferably, the total length of the continuous external triangular expanding focusing flow channel 5 is 200 mm-2000 mm; the width of the continuous outer triangular expansion focusing flow channel 5 is 100-200 μm; the distance between two adjacent channels of the continuous outer triangular expanding focusing channel 5 is 100-400 μm; the curvature radius of the innermost flow passage of the continuous outer triangular expanding focusing flow passage 5 is 20 mm-50 mm.

Preferably, the width of the triangular wall surface and the lower smooth wall surface of the continuous outer triangular expanding focusing flow channel 5 is 100 μm to 300 μm, the triangular flow channel wall surface structure is an equilateral triangle, and the side length is 50 μm to 200 μm; the total length of the flow channel is 500 mm-5000 mm, the distance between two adjacent vortex focusing curved channels is 200 μm-500 μm, and the curvature radius of the innermost flow channel is 10 mm-100 mm;

preferably, the gas buffer chamber 10 is spaced from the wall of the liquid-phase flow path by a distance of 50 μm to 200 μm;

preferably, the mixed liquid phase flow channel 13 wraps the end of the continuous liquid phase flow channel 6 and the reaction liquid phase sample injection flow channel to form a coaxial flow, the width of the reaction liquid phase sample injection flow channel is 50 μm to 200 μm, the width of the end of the continuous liquid phase flow channel 6 is 100 μm to 200 μm, and the width of the mixed liquid phase flow channel 13 is 200 μm to 400 μm;

preferably, the continuous gas phase channel 9 has a width of 40 μm to 120 μm.

Referring to fig. 1 to 4, the present application further provides a microfluidic chip, which is characterized by comprising a chip body and a micro channel structure in the above embodiment; the micro-channel structure is arranged in the chip body.

Optionally, the material of the chip body is preferably PDMS which is a transparent material, and the PDMS can be directly observed and photographed by using a microscope.

As a further improvement, the chip body of the microfluidic chip provided in the embodiment of the present application includes a substrate 14 and a cover plate 15; the micro flow channel structure is arranged on the upper surface of the substrate 14; the cover plate 15 covers the upper surface of the substrate 14, and the continuous liquid phase sample inlet 4, the gas phase sample inlet 8, the reaction liquid phase sample inlet 11 and the mixed phase sample outlet 22 are all communicated with the cover plate 15.

As a further improvement, the microfluidic chip provided in the embodiment of the present application further includes a conveying device and an extracting device 19; the conveying device comprises a first conveying pump 16 communicated with the continuous liquid phase sample inlet 4, a second conveying pump 17 communicated with the gas phase sample inlet 8 and a third conveying pump 18 communicated with the reaction liquid phase sample inlet 11; the extraction device 19 communicates with the mixed phase outlet 22. Wherein, the first delivery pump 16 is used for delivering the sample into the continuous liquid phase sample inlet 4; the second delivery pump 17 is used for delivering the source to enter the gas phase sample inlet 8; the third transfer pump 18 is configured to transfer the reaction liquid into the reaction liquid phase injection port 11, specifically, since the reaction liquid phase injection port 11 and the reaction liquid phase flow channel 12 are both preferably two, and one reaction liquid phase injection port 11 is matched with one reaction liquid phase flow channel 12, the third transfer pump 18 may be preferably two, and each third transfer pump 18 may be respectively communicated with one reaction liquid phase injection port 11, and is configured to transfer the same or different reaction liquids to the corresponding reaction liquid phase injection ports 11.

The micro-fluidic chip provided by the application has the following advantages:

1. the device structure is miniaturized. The whole chip device has small area and large specific surface area. The delivery device and the extraction device 19 work in concert to achieve high throughput.

2. High dispersion stability. The continuous external triangle expansion focusing flow passage 5 is more beneficial to passive dean flow inertial focusing, and the particle dispersion stability is increased.

3. The quantitative control is accurate. The microspheres passing through the focusing curve have uniform height and spacing values, and the quantity of particles (or liquid drops) in a continuous liquid phase can be quickly and accurately controlled by adjusting the pneumatic pump, so that accurate and controllable quantitative control is realized.

4. Easy to observe. The device can select PDMS of transparent material for use as the chip material, can directly use the microscope to observe, take a picture the record.

5. The application field is wide. Due to the spacing between the gas phase channel 9 and the liquid phase flow channel, the gas does not react any more on the particles in the reaction liquid, which is suitable for many heterogeneous reactions.

6. The cost is low. In the operation process, only less particles and reaction liquid are needed to achieve uniform and sufficient reaction which is difficult to achieve by conventional operation.

The present application also provides a heterogeneous reaction method applied to the micro flow channel structure of any one of claims 1 to 6, comprising the steps of:

s1, uniformly and stably dispersing the microsphere suspension liquid through the continuous outer triangular expansion focusing flow channel 5 and flowing the microsphere suspension liquid into a continuous liquid phase channel;

s2, adjusting the opening and closing of the continuous liquid phase channel through the gas phase channel 9 of the active valve quantitative and uniform control unit, and accurately controlling the quantity of microspheres in the microsphere suspension flowing into the coaxial flow heterogeneous reaction unit;

and S3, enabling the reaction liquid to enter the mixed liquid phase flow channel 13 through the reaction liquid phase flow channel 12, and mixing and reacting with the microsphere suspension in the mixed liquid phase flow channel 13.

The above is the first embodiment provided by the present application, and the following is the second embodiment provided by the present application, specifically:

the chip body is made of PDMS, wherein the length of the continuous outer triangular expansion focusing flow channel 5 is 1000mm, the distance between two adjacent vortex focusing flow channels is 150 μm, the curvature radius of the innermost flow channel is 20mm, the widths of the gas phase flow channel and the reaction liquid phase flow channel 12 are 50 μm, the width of the continuous liquid phase flow channel 6 is 100 μm, the width of the mixed liquid phase flow channel 13 is 200 μm, a certain distance is kept between the gas buffer chamber 10 and the liquid phase flow channel wall, the distance is 40 μm, and the heights of all the flow channels are 100 μm. Nitrogen is selected as gas phase, 50 percent alcohol solution containing polystyrene microspheres with the particle size of 30 mu m is selected as sample solution, and deionized water solution is selected as reaction sample solution. The fluid was injected into the chip body using a teflon capillary hose, and the gas phase fluid was controlled using the second transfer pump 17. The flow rate of the sample liquid is 30 mul/min, the flow rate of the gas phase is 50 mul/min, and the flow rate of the reaction liquid phase is 30 mul/min, liquid phases with various quantitative microsphere contents can be obtained by adjusting the flow rates of the gas phase and the continuous phase liquid phase, and the quantitative heterogeneous reaction is completed by mixing the coaxial flow and the reaction liquid.

The above is the second embodiment provided by the present application, and the following is the third embodiment provided by the present application, specifically:

the chip body is made of PDMS, wherein the length of the continuous outer triangular expansion focusing flow channel 5 is 1100mm, the distance between two adjacent vortex focusing flow channels is 130 μm, the curvature radius of the innermost flow channel is 15mm, the widths of the gas phase flow channel and the reaction liquid phase flow channel 12 are 60 μm, the width of the continuous liquid phase flow channel 6 is 100 μm, the width of the mixed liquid phase flow channel 13 is 180 μm, a certain distance is kept between the gas buffer chamber 10 and the liquid phase flow channel wall and is 50 μm, and the heights of all the flow channels are 100 μm. Nitrogen is selected as gas phase, modified titanium dioxide solution with the particle size of 50 mu m is selected as sample solution, and deionized water solution is selected as reaction sample solution. The fluid was injected into the chip using a teflon capillary hose, and the gas phase fluid was controlled by a second transfer pump 17 pump. The flow rate of the sample liquid is 20 mul/min, the flow rate of the gas phase is 50 mul/min, and the flow rate of the reaction liquid phase is 20 mul/min, liquid phases with various quantitative microsphere contents can be obtained by adjusting the flow rates of the gas phase and the continuous phase liquid phase, and the quantitative heterogeneous reaction is completed by mixing the coaxial flow and the reaction liquid.

The above embodiments are only used for illustrating the technical solutions of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present application.