阅读说明：本技术 一种植物促生长防病防虫药肥制备方法 (Preparation method of plant growth-promoting disease-preventing insect-preventing pesticide fertilizer ) 是由 宋兴舜 王玉龙 张淑梅 于 2021-01-21 设计创作，主要内容包括：本发明涉及一种植物促生长防病防虫药肥制备方法,原料以及所占比例为：S1营养调节剂质量百分比10％：S2复合功能菌剂质量百分比86％,S3植物源杀虫质量百分比2％,S4复合酶制剂质量百分比2％；将上述各原料分别混拌均匀,再依次将S1营养调节剂、S3植物源杀虫、S4复合酶制剂、溶于S2混合均匀,灌装即成。本发明为植物特别是粮食作物提供氮素、提高产量、降低化肥用量和生产成本、建立生态平衡和促进农业可持续发展具有重要意义,具有高效防治多种作物病虫害,提高作物免疫力,促进作物根系发育,增产提质,绿色环保的多重作用。(The invention relates to a preparation method of a plant growth-promoting disease-preventing insect-preventing pesticide fertilizer, which comprises the following raw materials in percentage by weight: s1 nutrient regulator 10% by mass: 86% of S2 composite functional microbial inoculum, 2% of S3 botanical insecticidal and 2% of S4 composite enzyme preparation; mixing the above materials respectively, adding S1 nutrition regulator, S3 botanical pesticide, S4 complex enzyme preparation, dissolving in S2, mixing, and packaging. The invention provides nitrogen for plants, particularly grain crops, improves the yield, reduces the fertilizer consumption and the production cost, establishes ecological balance and promotes agricultural sustainable development, has important significance, and has multiple functions of efficiently preventing and treating various crop diseases and insect pests, improving the crop immunity, promoting the crop root system development, increasing the yield and improving the quality, and being green and environment-friendly.)

1. A preparation method of a plant growth-promoting disease-preventing insect-preventing pesticide fertilizer is characterized by comprising the following steps: the raw materials and the proportion are as follows: s1 nutrient regulator 10% by mass: 86% of S2 composite functional microbial inoculum, 2% of S3 botanical insecticidal and 2% of S4 composite enzyme preparation; respectively mixing the above raw materials, mixing, dissolving S1 nutrition regulator, S3 botanical pesticide, S4 complex enzyme preparation in S2, mixing, and packaging, wherein the S1 nutrition regulator is prepared by: the S1 nutrition regulator comprises the following components in percentage by mass in S1: 2 percent of compound phthalein nucleic acid, 2 percent of nucleotide, 1 percent of proline, 1 percent of glycine, 1 percent of glutamic acid, 1 percent of glutamine, 1 percent of arginine, 1 percent of alanine, 7 percent of inosine, 0.5 percent of cytokinin, 1 percent of potassium indolebutyrate, 0.5 percent of S-elicitor, 1 percent of brassinolide, 10 percent of glucose, 10 percent of soybean peptide hydrolysate, 10 percent of biochemical fulvic acid, 2 percent of chelated rare earth trace element, 0.5 percent of chelated boron, 0.3 percent of chelated calcium, 0.3 percent of chelated zinc, 0.9 percent of sugar alcohol silicon, 1 percent of nano titanium and 0.1 percent of trace element, and the components are mixed for later use.

2. The method for preparing the plant growth-promoting, disease-preventing and insect-preventing pesticide fertilizer according to claim 1, which is characterized in that: s2 preparation of a composite functional bacterial agent: the composite functional bacteria comprise the following components in percentage by mass in S2: rhodopseudomonas palustris Rhodopseudomonas stum Rhodopseudomonas palustris (bacterial number: ACCC10149) accounts for 87% of the mass of the composite functional bacteria agent, and the mass of other combined bacteria accounts for 13%; other combinations of bacteria include: 1 percent of Bacillus pumilus (bacterial No. ACCC10113), 1 percent of Azotobacter chroococcum (bacterial No. ACCC11104), 1 percent of Bacillus laterosporus Laubach (bacterial No. ACCC11079), and 1 percent of Candida tropicalis bacterial No: ACCC20275), 1% by mass, Bacillus subtilis (bacterial number: ACCC10632), Bacillus amyloliquefaciens (bacterial number: ACCC10225), bacillus polymyxa Paenibacillus polymyxa (bacterial number: ACCC1010122), Bacillus licheniformis (bacterial number: ACCC11080), pseudomonas fluorescens (ACCC10040) 1%, Trichoderma harchisonium (bacterial number: ACCC30371), 1 percent by mass of Bacillus firmus I-1582, 1 percent by mass of Streptomyces microflavus (bacterial number: ACCC40058) and 1 percent of short-stable bacillus, wherein the bacillus pumilus, azotobacter chroococcum, bacillus laterosporus, candida tropicalis, bacillus subtilis, bacillus amyloliquefaciens, bacillus polymyxa, bacillus licheniformis, pseudomonas fluorescens, trichoderma harzianum, bacillus firmus I-1582, streptomyces microflavus and short-stable bacillus are bacteria powder provided by the China agricultural microbial strain preservation management center. The rhodopseudomonas palustris liquid is fermented into self-expanded propagation, the effective bacteria count is more than 1 multiplied by 1010cfu g < -1 >, all the bacteria powder is uniformly mixed according to the mass ratio of 1: 1 of each substance in the composition, and then the mixture is dissolved in the rhodopseudomonas palustris liquid for later use.

3. The method for preparing the plant growth-promoting, disease-preventing and insect-preventing pesticide fertilizer according to claim 1, which is characterized in that: s3 botanical insecticide: the botanical pesticide comprises the following components in S3 by mass percent: 40% of ganoderma lucidum glucan, 40% of inonotus obliquus glucan, 10% of azadirachtin and 10% of chitosan, and the ganoderma lucidum glucan, the inonotus obliquus glucan, the azadirachtin and the chitosan are mixed and stirred uniformly for later use.

4. The method for preparing the plant growth-promoting, disease-preventing and insect-preventing pesticide fertilizer according to claim 1, which is characterized in that: preparation of S4 enzyme preparation: the mass percentage of each enzyme preparation component in S4 is as follows: 10% of cellulase, 10% of lipase, 10% of acid protease, 10% of a-amylase, 10% of phytase, 10% of glucose oxidase, 10% of xylanase, 10% of beta-glucanase and 10% of pectinase, 1% of superoxide dismutase SOD and 102% of coenzyme Q: glutathionase 3%: hypersensitive protease 4%; mixing cellulase, lipase, acid protease, alpha-amylase, phytase, glucose oxidase, xylanase, beta-glucanase and pectinase, superoxide dismutase SOD, coenzyme Q10: glutathione enzyme: mixing the hypersensitive protease uniformly for later use.

Technical Field

The invention relates to a preparation method of a plant growth-promoting disease-preventing insect-preventing pesticide fertilizer.

Background

The essence of the rice seedling raising and thin planting cultivation technology is as follows: the dry field soil which is fertile and loose is adopted, under the environment of the drought seedbed, the cultivated rice seedlings have strong and developed root systems, short and thick stems and leaves and strong stems, and the rice seedlings with low-position tillers are transplanted into the environment with water in the field, quickly take roots and turn green, and early tillering and multiple tillers depend on reasonable effective tillering spike numbers in the conditions of long plant spacing/interval, sufficient illumination and good ventilation, thereby realizing the high quality and high yield of the rice. Although the rice absorbs trace elements in a small amount, the effect of the trace elements is very important. The trace elements such as zinc and boron are reported to improve the oxygen supply of rice roots, enhance the stress resistance of rice plants, improve the disease resistance of the rice plants, promote the development of later-stage root systems, prolong the functional period of leaves and prevent premature senility; the growth of flowers can be accelerated, the number of pollen is increased, the germination of flower grains is promoted, and the improvement of the rice heading rate is facilitated; but also can promote more ears and grains, improve the seed setting rate and the grain filling degree, thereby increasing the rice yield. The compound enzyme preparation is used for enhancing the absorption and utilization of the regulator in the seedling turning and tillering stage of the rice seedlings, protecting the root systems, degrading pesticide residues and overcoming the damage of the current soil-sealing pesticide to the root systems of the rice seedlings.

At present, the agricultural seedbed nutrient has a single patent technical scheme, the advantages of microbial flora are not obvious, the biological control effect is not obvious, the advantages cannot be complemented, and the agricultural seedbed nutrient is compounded by various products for farmers to treat diseases in the existing market and has extremely high cost. The conventional foreign technologies such as Bibao application of gibberellin, indoleacetic acid and brassinolide hormones to stimulate the growth of crops have certain short-term effects, but the growth of the crops in the later period is more premature senility caused by hormone roots, the first right hormone is only a product synthesized by amino acid and medium trace elements, functional microorganisms and plant source sterilization and insecticidal conditioning agents are not contained, and the effect is single. According to the conventional technology in China, amino acid and humic acid liquid fertilizer are mostly used in the aspect of seedbed nutrition, only trichoderma harzianum and bacillus subtilis microbial agents are applied to a rice seedbed for preventing diseases and promoting growth, the prevention effect is not ideal, the biological flora is single, and the disease prevention mechanism is simple. After the disease occurs, chemical metalaxyl-M, hymexazol and fludioxonil are used, the treatment effect is poor, and people advocate to treat the disease before the disease is treated, prevent the disease more than treat the disease, neutralize and regulate the disease, and consolidate the constitution and the origin. In the aspect of comprehensive prevention and control, no biological enzyme method, plant source prevention and control, gene regulation and control and microorganism organic combination are achieved, so that a product with comprehensive functions and obvious application effect is urgently needed in the agricultural and welfare market.

The invention content is as follows:

the invention aims to provide a preparation method of a disease-preventing and insect-preventing pesticide fertilizer for promoting plant growth, which not only contains organic components required by crop growth, but also has the effects of preventing diseases and promoting growth, and can improve the physical and chemical properties of soil.

The technical scheme of the invention is as follows: a preparation method of a plant growth-promoting disease-preventing insect-preventing pesticide fertilizer comprises the following steps: the raw materials and the proportion are as follows: s1 nutrient regulator 10% by mass: 86% of S2 composite functional microbial inoculum, 2% of S3 botanical insecticidal and 2% of S4 composite enzyme preparation; mixing the above materials respectively, dissolving S1 nutrition regulator, S3 botanical pesticide, S4 complex enzyme preparation in S2, mixing, and packaging; preparation of S1 nutrient regulator: the S1 nutrition regulator comprises the following components in percentage by mass in S1: 2% of composite phthalein nucleic acid, 2% of nucleotide, 1% of proline, 1% of glycine, 1% of glutamic acid, 1% of glutamine, 1% of arginine, 1% of alanine, 7% of inosine, 0.5% of cytokinin, 1% of potassium indolebutyrate, 0.5% of S-elicitor, 1% of brassinolide, 10% of glucose, 10% of soybean peptide hydrolysate, 10% of biochemical fulvic acid, 2% of chelated rare earth trace elements, 0.5% of chelated boron, 0.3% of chelated calcium, 0.3% of chelated zinc, 0.9% of sugar alcohol silicon, 1% of nano titanium and 0.1% of trace elements, and mixing uniformly for later use.

S2 preparation of a composite functional bacterial agent: the composite functional bacteria comprise the following components in percentage by mass in S2: rhodopseudomonas palustris Rhodopseudomonas stum Rhodopseudomonas palustris (bacterial number: ACCC10149) accounts for 87% of the mass of the composite functional bacteria agent, and the mass of other combined bacteria accounts for 13%; other combinations of bacteria include: 1 percent of Bacillus pumilus (bacterial No. ACCC10113), 1 percent of Azotobacter chroococcum (bacterial No. ACCC11104), 1 percent of Bacillus laterosporus Laubach (bacterial No. ACCC11079), and 1 percent of Candida tropicalis bacterial No: ACCC20275), 1% by mass, Bacillus subtilis (bacterial number: ACCC10632), Bacillus amyloliquefaciens (bacterial number: ACCC10225), bacillus polymyxa Paenibacillus polymyxa (bacterial number: ACCC1010122), Bacillus licheniformis (bacterial number: ACCC11080), pseudomonas fluorescens (ACCC10040) 1%, Trichoderma harchisonium (bacterial number: ACCC30371), 1 percent by mass of Bacillus firmus I-1582, 1 percent by mass of Streptomyces microflavus (bacterial number: ACCC40058) and 1 percent of short stable bacillus, bacillus pumilus and bacillus pumilus,The azotobacter chroococcum, bacillus laterosporus, candida tropicalis, bacillus subtilis, bacillus amyloliquefaciens, bacillus polymyxa, bacillus licheniformis, pseudomonas fluorescens, trichoderma harzianum, bacillus firmus I-1582, streptomyces microflavus and short-stable bacillus are bacteria powder provided by the China agricultural microbial strain preservation management center, the rhodopseudomonas palustris liquid fermentation is self-expanded propagation, and the effective bacteria number is 1 multiplied by 10¹⁰More than cfu g < -1 >, uniformly mixing all the bacterial powder according to the mass ratio of 1: 1 of all the substances in the composition, and dissolving the mixture in the rhodopseudomonas palustris solution for later use.

S3 botanical insecticide: the botanical pesticide comprises the following components in S3 by mass percent: 40% of ganoderma lucidum glucan, 40% of inonotus obliquus glucan, 10% of azadirachtin and 10% of chitosan, and the ganoderma lucidum glucan, the inonotus obliquus glucan, the azadirachtin and the chitosan are mixed and stirred uniformly for later use.

Preparation of S4 enzyme preparation: the mass percentage of each enzyme preparation component in S4 is as follows: 10% of cellulase, 10% of lipase, 10% of acid protease, 10% of a-amylase, 10% of phytase, 10% of glucose oxidase, 10% of xylanase, 10% of beta-glucanase and 10% of pectinase, 1% of superoxide dismutase SOD and 102% of coenzyme Q: glutathionase 3%: hypersensitive protease 4%; mixing cellulase, lipase, acid protease, alpha-amylase, phytase, glucose oxidase, xylanase, beta-glucanase and pectinase, superoxide dismutase SOD, coenzyme Q10: glutathione enzyme: mixing the hypersensitive protease uniformly for later use.

Technical effects of the invention

According to the disease control theory of biodiversity, the complementary action of different microbial floras is realized through deep experimental research and repeated production practice. The microbial biological control and biological enzyme technology and the biochemical control are combined and applied to agricultural production, carbon and nitrogen are supplemented, the soil is improved by microorganisms, salt and water are balanced, and a soil environment beneficial to healthy growth of plants is created, so that the soil fertility is increased, the growth of crops is promoted, strong seedlings are cultivated, tillering and transplanting are carried out, no striking root fertilizer is used, the investment of farmers is reduced, labor and time are saved, and the yield is increased.

First, the advantage of the complex enzyme preparation, also called enzyme, is a high molecular substance with life catalysis, and organisms contain thousands of enzymes which dominate many catalytic processes of metabolism, nutrition and energy conversion of organisms, and most of the reactions closely related to life processes are enzyme-catalyzed reactions, and no life exists without enzymes. The enzyme is a catalyst for metabolism in organisms, and can carry out various biochemical reactions in the organisms only in the presence of the enzyme. In the cell, the synthesis of enzymes is also closely related to nutrients and metabolites. The intake of certain nutrients, especially of some trace elements, can promote the synthesis of the corresponding enzymes. Therefore, the enzyme and the medium-trace elements are said to supplement each other. On one hand, the fertilizer is good in absorption and utilization, saves the process of absorbing and manufacturing nutrients by crops, and on the other hand, reduces the nutrient consumption of the crops. The complex enzyme can be directly absorbed by plants, so that the physical ability of the plants required in the conversion of trace elements can be saved, the growth and development of the plants are greatly promoted, and the disease resistance, oxidation resistance and aging resistance can be improved.

Secondly, the compound phthalein nucleic acid is a pure green plant extract and is known as the sixth vital essence of green plants; the first choice of the spraying matter on the leaf surfaces of A-grade and AA-grade agricultural products; the 'food safety law' of implementation advocates the yield increase of crops, green fertilization and pesticide application. The composite phthalein nucleic acid is a new type plant growth regulator

(one) toxicity of Complex phthalein nucleic acids: the pure natural plant extract is safe to human and livestock, and has no residue and pollution to environment and organisms.

Overview of (di) Complex phthalein nucleic acids: the compound phthalein nucleic acid is a novel plant growth substance, can induce plant gene active enzyme, inspires growth factors to promote crop growth, and is known as the sixth vital essence of green plants by the plant physiology world. Meanwhile, the compound phthalein nucleic acid has the advantages of environmental friendliness and strong compatibility, is the first choice for producing green agricultural products (A grade and AA grade), is different from various traditional chemical regulators, is a substitute product for the chemical regulators widely used in the market at present, and represents the development direction of plant regulating substances.

(III) characteristics of complex phthalein nucleic acid:

1. the composite phthalic acid promotes the elongation growth of plants, promotes the growth of rhizomes and buds, enables seedlings and growing plants to grow fast to form strong seedlings and strong plants, and increases the weight of leaf and stem plants.

2. The compound phthalein nucleic acid can prolong leaf aging and promote the reduction rate of chlorophyll, protein and protoplasm contents.

3. The composite phthalic acid has strong chemical stability, can be mixed with various acidic or alkaline (PH 4-9) fertilizers and pesticides for use, and overcomes the defects of poor mixing property and antagonistic property of the traditional chemical regulator.

4. The compound phthalein nucleic acid has broad spectrum, high efficiency and environmental protection: the product is suitable for all plants in the whole growth period, and can replace traditional chemical regulators including: sodium complex and sodium naphthalene.

The proline, glycine, glutamic acid, glutamine, arginine and alanine compound amino acid can increase the synthesis of hormone in plants, increase tillering of plants, improve the content of chlorophyll, reduce the degradation speed of chlorophyll, improve the photosynthetic rate and promote the development of plants. The addition of the sodium nucleotide can further enhance the tillering effect, and the nucleotide can improve the absorption capacity of the root system and promote metabolism. The synergistic effect of the compound amino acid can obviously increase the tillering number, and has the advantages of fast seedling recovering, early tillering, developed plant root system and greatly improved disease resistance.

The functional flora contained in the invention is that the bacillus pumilus can hydrolyze starch, degrade mannan, xylan and cellulose to prevent and control wheat root rot and strawberry gray mold. The azotobacter chroococcum has biological nitrogen fixation capacity, and has important significance in providing nitrogen for plants, particularly grain crops, improving the yield, reducing the fertilizer consumption and the production cost, establishing ecological balance and promoting agricultural sustainable development. The biocontrol flora is bacillus laterosporus, bacillus subtilis, bacillus amyloliquefaciens, bacillus polymyxa and trichoderma harzianum, and resists the infection of soil-borne fungi to crops through a nutrition site competition, a resistance substance secretion mechanism and a crop disease resistance induction mechanism. The streptomyces microflavus can also secrete active substances for promoting the growth of crops, so that the roots of the plants are deep and luxuriant, and the effects of disease resistance and yield increase are achieved. The bacillus firmus I-1582 has multiple functions of efficiently preventing and treating various crop diseases and insect pests, improving the immunity of crops, promoting the development of crop roots, increasing the yield and improving the quality, and is green and environment-friendly.

The rhodopseudomonas palustris has the function of fixing nitrogen and resisting virus bacteria, is a nitrogen fixing bacterium, can fix nitrogen in air and convert the nitrogen into nitrogen needed by crops, and greatly increases the number of the nitrogen fixing bacteria in soil treatment after the rhodopseudomonas palustris is applied, so that the nitrogen fixing effect is improved. The rhodopseudomonas palustris is a kind of root-growth-promoting bacterium, and after the rhodopseudomonas palustris is applied, the length of crop roots and the number of root hairs can be increased, so that the effect of the roots absorbing nutrients is greatly enhanced, which is another important mechanism for promoting the yield increase of crops. The rhodopseudomonas palustris can metabolize a plurality of important substances such as proline, uracil, cytosine, vitamin, coenzyme Q and carotenoid which have the effect of remarkably promoting the growth of crops, and can be directly absorbed by the crops, and more importantly, the quality of the crops and fruits can be greatly improved, and the effect of applying phytohormones is far better than that of applying phytohormones. Rhodopseudomonas palustris reduces nitrate into amino acid or ammonia gas through nitrate reductase and nitrite reductase, thereby greatly reducing nitrite content in fruit and vegetable grains, being matched with beneficial bacillus strains which are beneficial to crop growth and inhibit pathogenic bacteria, and having better effects of increasing yield and resisting diseases. The disease probability of crops can be greatly reduced after the rhodopseudomonas palustris is applied. The most important function generated when crops are applied to rhodopseudomonas palustris is that the number of rhizosphere-promoting bacteria can be greatly increased, and various antibiotics secreted by actinomycetes after the number of the actinomycetes is increased are mostly generated by the actinomycetes in the prior agricultural various antibiotic products for sterilization, so that the effect of inhibiting pathogenic bacteria is achieved, and meanwhile, the disease resistance of the crops can be induced and improved.

In conclusion, the invention mainly adopts the measures of synergistic disease prevention with active biological bacteria and enzyme, carbon supplement enzyme and other non-antagonistic microorganisms. The composite flora overcomes the defect that the composite flora competes with other microbial communities in the process of colonization in the soil ecological environment to form an advantageous population with enough population quantity to overcome the rejection of soil inhabiting microbes including pathogenic bacteria, keep the soil inhabiting microbes colonized around plant roots and leaves, secrete various enzymes and antibiotics, induce the disease resistance of plants, simultaneously play the advantages of high insecticidal and bacteriostatic speed of biological pesticides and stable control effect and achieve the aims of complementary advantages, reduced dosage and enhanced control effect through compounding and synergism. The synergistic interaction with other antagonistic microorganisms is that different antibiotic bacteria which are mixed by more than two mutually fused antagonistic microorganisms are mixed without antagonistic, parasitic, dissolving or competing mutual exclusion relations, so that the space of different parts of the plant can be fully occupied by the mixed strain, and the mixed bacteria can grow and propagate in the amplitude of larger light, temperature, gas and humidity due to the fact that the mixed bacteria can adapt to different environmental conditions, so that the synergistic disease control effect of function complementation, prevention and treatment of various antibiotics and lasting effect can be expected to be realized, and the biological control bacteria can be mixed with other functional bacteria to prepare a biological control agent for use. According to the invention, the biocontrol bacteria of beneficial bacteria of bacillus pumilus, bacillus amyloliquefaciens, bacillus polymyxa, bacillus licheniformis, pseudomonas fluorescens, trichoderma harzianum, bacillus firmus I-1582 and streptomyces microflavus, the compound biological enzyme and the plant source extract which has the functions of disinsection, sterilization and nutrition supplementation are added, so that the biological flora nutrient not only contains organic components required by crop growth, but also has the effects of preventing diseases and promoting growth, and can improve the physicochemical property of soil, and is a multifunctional biological flora nutrient for preventing and treating plant diseases.

The specific implementation mode is as follows:

a preparation method of a plant growth-promoting disease-preventing insect-preventing pesticide fertilizer comprises the following steps: the raw materials and the proportion are as follows: s1 nutrient regulator 10% by mass: 86% of S2 composite functional microbial inoculum, 2% of S3 botanical insecticidal and 2% of S4 composite enzyme preparation; mixing the above materials respectively, dissolving S1 nutrition regulator, S3 botanical pesticide, S4 complex enzyme preparation in S2, mixing, and packaging;

preparation of S1 nutrient regulator: the S1 nutrition regulator comprises the following components in percentage by mass in S1: 2% of composite phthalein nucleic acid, 2% of nucleotide, 1% of proline, 1% of glycine, 1% of glutamic acid, 1% of glutamine, 1% of arginine, 1% of alanine, 7% of inosine, 0.5% of cytokinin, 1% of potassium indolebutyrate, 0.5% of S-elicitor, 1% of brassinolide, 10% of glucose, 10% of soybean peptide hydrolysate, 10% of biochemical fulvic acid, 2% of chelated rare earth trace elements, 0.5% of chelated boron, 0.3% of chelated calcium, 0.3% of chelated zinc, 0.9% of sugar alcohol silicon, 1% of nano titanium and 0.1% of trace elements, and mixing uniformly for later use.

S2 preparation of a composite functional bacterial agent: the composite functional bacteria comprise the following components in percentage by mass in S2: rhodopseudomonas palustris Rhodopseudomonas stum Rhodopseudomonas palustris (bacterial number: ACCC10149) accounts for 87% of the mass of the composite functional bacteria agent, and the mass of other combined bacteria accounts for 13%; other combinations of bacteria include: 1 percent of Bacillus pumilus (bacterial No. ACCC10113), 1 percent of Azotobacter chroococcum (bacterial No. ACCC11104), 1 percent of Bacillus laterosporus Laubach (bacterial No. ACCC11079), and 1 percent of Candida tropicalis bacterial No: ACCC20275), 1% by mass, Bacillus subtilis (bacterial number: ACCC10632), Bacillus amyloliquefaciens (bacterial number: ACCC10225), bacillus polymyxa Paenibacillus polymyxa (bacterial number: ACCC1010122), Bacillus licheniformis (bacterial number: ACCC11080), pseudomonas fluorescens (ACCC10040) 1%, Trichoderma harchisonium (bacterial number: ACCC30371), 1 percent by mass of Bacillus firmus I-1582, 1 percent by mass of Streptomyces microflavus (bacterial number: ACCC40058) and 1 percent of short stable bacillus, and the required brevibacillus pumilusBacillus, azotobacter chroococcum, bacillus laterosporus, candida tropicalis, bacillus subtilis, bacillus amyloliquefaciens, bacillus polymyxa, bacillus licheniformis, pseudomonas fluorescens, trichoderma harzianum, bacillus firmus I-1582, streptomyces microflavus and bacillus brevis are bacteria powder provided by the China agricultural microbial strain preservation management center, the rhodopseudomonas palustris liquid fermentation is self-expanded propagation, and the effective bacteria number is 1 multiplied by 10¹⁰More than cfu g < -1 >, uniformly mixing all the bacterial powder according to the mass ratio of 1: 1 of all the substances in the composition, and dissolving the mixture in the rhodopseudomonas palustris solution for later use.

S3 botanical insecticide: the botanical pesticide comprises the following components in S3 by mass percent: 40% of ganoderma lucidum glucan, 40% of inonotus obliquus glucan, 10% of azadirachtin and 10% of chitosan, and the ganoderma lucidum glucan, the inonotus obliquus glucan, the azadirachtin and the chitosan are mixed and stirred uniformly for later use.

Preparation of S4 enzyme preparation: the mass percentage of each enzyme preparation component in S4 is as follows: 10% of cellulase, 10% of lipase, 10% of acid protease, 10% of a-amylase, 10% of phytase, 10% of glucose oxidase, 10% of xylanase, 10% of beta-glucanase and 10% of pectinase, 1% of superoxide dismutase SOD and 102% of coenzyme Q: glutathionase 3%: hypersensitive protease 4%; mixing cellulase, lipase, acid protease, alpha-amylase, phytase, glucose oxidase, xylanase, beta-glucanase and pectinase, superoxide dismutase SOD, coenzyme Q10: glutathione enzyme: mixing the hypersensitive protease uniformly for later use.