阅读说明：本技术 草莓用复合菌及其制备方法和应用 (Compound bacterium for strawberries as well as preparation method and application thereof ) 是由 焦国宝 朱丽娜 孙宁宁 高璇璇 陈晓宇 崔红健 王渝鹭 曹忠洋 张永钦 朱飞帆 于 2021-03-16 设计创作，主要内容包括：本发明提供了一种草莓用复合菌及其制备方法和应用,涉及水果病害防治的技术领域。本发明提供的草莓用复合菌,包括细菌、真菌和载体；其中细菌、真菌和载体的质量比为35～60:3～4:30～50。所述细菌包括井岗·枯草芽孢杆菌、侧孢芽孢杆菌、甲基营养型芽孢杆菌、沼泽红假单孢菌和蜡样芽孢杆菌,其质量比为60～100：4～8：4～6：4～6：2～3。本发明通过微生物菌群中细菌和真菌协同作用,抢占致病菌的空间及养分,大量繁殖,使白粉病原菌菌丝畸形,从而抑制病原菌的生长。而且微生物分泌生理活性物质,促进植株生长,降低了化肥使用量,降低种植成本。(The invention provides a composite bacterium for strawberries and a preparation method and application thereof, and relates to the technical field of fruit disease control. The composite bacteria for the strawberries provided by the invention comprise bacteria, fungi and a carrier; wherein the mass ratio of the bacteria to the fungi to the carrier is 35-60: 3-4: 30-50. The bacteria comprise Jinggang bacillus subtilis, bacillus laterosporus, bacillus methylotrophicus, rhodopseudomonas palustris and bacillus cereus, and the mass ratio of the bacillus laterosporus to the bacillus cereus is 60-100: 4-8: 4-6: 4-6: 2 to 3. The invention preempts the space and the nutrients of pathogenic bacteria and performs mass propagation by the synergistic action of bacteria and fungi in microbial flora, so that the hypha of powdery mildew pathogenic bacteria are deformed, thereby inhibiting the growth of the pathogenic bacteria. And the microorganisms secrete physiological active substances, so that the growth of plants is promoted, the use amount of chemical fertilizers is reduced, and the planting cost is reduced.)

1. The composite bacterium for the strawberries is characterized by comprising bacteria, fungi and a carrier; wherein the mass ratio of the bacteria to the fungi to the carrier is 35-60: 3-4: 30-50;

wherein the bacteria include Bacillus subtilis, Bacillus laterosporus, Bacillus methylotrophicus, Rhodopseudomonas palustris and Bacillus cereus.

2. The composite bacterium for strawberries according to claim 1, wherein the mass ratio of the bacteria Bacillus subtilis, Bacillus laterosporus, Bacillus methylotrophicus, Rhodopseudomonas palustris and Bacillus cereus is 60-100: 4-8: 4-6: 4-6: 2 to 3.

3. The composite bacterium for strawberries according to claim 1, wherein the fungus comprises pythium oligandrum.

4. The complex bacterium for strawberry according to claim 1, wherein the carrier comprises potassium fulvate;

preferably, the potassium fulvate is mineral potassium fulvate.

5. The composite bacterium for strawberries as claimed in claim 1, wherein the effective viable count of the Jinggang-Bacillus subtilis is 1-5% validamycin-200-1000 hundred million/g.

6. The composite bacterium for strawberry as claimed in claim 1, wherein the effective viable count of Bacillus laterosporus is 200-1000 hundred million/g;

preferably, the effective viable count of the bacillus methylotrophicus is 80-200 hundred million/g;

preferably, the effective viable count of the rhodopseudomonas palustris is 80-200 hundred million/g;

preferably, the effective viable count of the bacillus cereus is 80-200 hundred million/g.

7. The complex bacterium for strawberry as claimed in claim 3, wherein the Pythium oligandrum has an effective viable count of 100-200 million/g.

8. A method of producing a composite bacterium for strawberry according to any one of claims 1 to 7, wherein the bacterium, the fungus and the carrier are uniformly mixed to obtain the composite bacterium for strawberry.

9. Use of the composite bacterium for strawberry according to any one of claims 1 to 7 or the composite bacterium for strawberry prepared by the preparation method according to claim 8 in strawberry cultivation.

10. The use of the complex bacterium for strawberries according to claim 9, wherein the application is carried out by a method of furrow application and spray application.

Technical Field

The invention relates to the technical field of fruit disease control, and particularly relates to a composite bacterium for strawberries as well as a preparation method and application thereof.

Background

Strawberry powdery mildew is one of the most main diseases in strawberry production, and is mainly harmful to strawberry leaves and fruits. After the fruits are affected with diseases, the surfaces of the fruits are covered with a layer of white powder, so that the fruits lose luster and harden, the quality is seriously affected, and the yield and the income are reduced. The existing strawberry powdery mildew prevention and treatment method mainly depends on drug prevention and treatment, or spraying plant extract, amino acid, sulfur fumigation and the like.

The existing control method mainly has the following defects:

1. the drug residue in soil and fruits is serious, and the seedling root is seriously damaged;

2. the fertilizer utilization rate is low, and soil hardening is serious;

3. low yield, low commodity rate and poor quality.

In view of the above, the present invention is particularly proposed.

Disclosure of Invention

One of the purposes of the invention is to provide a compound bacterium for strawberries, which is used for relieving serious drug residues in soil and fruits and serious seedling root damage in the existing strawberry powdery mildew prevention and control technology; the fertilizer utilization rate is low, and soil hardening is serious; low yield, low commodity rate and poor quality.

The second purpose of the invention is to provide the preparation method of the compound bacteria for the strawberries, which has mild conditions, does not harm microorganisms and ensures the activity of the microorganisms in the compound bacteria.

The invention also aims to provide the application of the composite bacteria for strawberries in strawberry planting, and improve the commodity rate, fruit quality and yield of strawberries.

In order to solve the technical problems, the invention adopts the following technical scheme:

the invention provides a compound bacterium for strawberries, which comprises bacteria, fungi and a carrier; wherein the mass ratio of the bacteria to the fungi to the carrier is 35-60: 3-4: 30-50;

wherein the bacteria include Bacillus subtilis, Bacillus laterosporus, Bacillus methylotrophicus, Rhodopseudomonas palustris and Bacillus cereus.

Further, the mass ratio of the bacillus subtilis, the bacillus laterosporus, the bacillus methylotrophicus, the rhodopseudomonas palustris and the bacillus cereus in the bacteria is 60-100: 4-8: 4-6: 4-6: 2 to 3.

Further, the fungus comprises pythium oligandrum.

Further, the carrier comprises potassium fulvate;

preferably, the potassium fulvate is mineral potassium fulvate.

Furthermore, the effective viable count of the Jinggang-Bacillus subtilis is 1-5% of validamycin-200-1000 hundred million/g.

Further, the effective viable count of the bacillus laterosporus is 200-1000 hundred million/g;

preferably, the effective viable count of the bacillus methylotrophicus is 80-200 hundred million/g;

preferably, the effective viable count of the rhodopseudomonas palustris is 80-200 hundred million/g;

preferably, the effective viable count of the bacillus cereus is 80-200 hundred million/g.

Further, the effective viable count of the pythium oligandrum is 100-200 hundred million/g.

The invention also provides a preparation method of the composite bacterium for strawberries, which is characterized in that the bacterium, the fungus and the carrier are uniformly mixed to obtain the composite bacterium for strawberries.

The third aspect of the invention provides application of the composite bacteria for strawberries in strawberry cultivation.

Further, the application is carried out by a method of furrow application and spray application.

The composite bacteria for strawberries preempt space and nutrients of pathogenic bacteria and propagate in a large amount through the synergistic effect of bacteria and fungi in microorganisms, so that hyphae of powdery mildew pathogenic bacteria are deformed, and the growth of the pathogenic bacteria is inhibited. And the microorganisms secrete physiological active substances such as auxin, vitamins, amino acid and the like, so that the growth of plants is promoted, the using amount of the fertilizer is reduced, the fertilizer efficiency is improved, and the planting cost is reduced. The composite bacteria promote the propagation of beneficial bacteria in soil, balance soil acid and alkali, break soil hardening and improve strawberry yield.

The preparation method of the composite bacteria for the strawberries provided by the invention has mild operation conditions, ensures the activity of the bacteria and the effectiveness of the product, and is suitable for large-scale industrial production.

The application of the composite bacteria for strawberries improves the quality of strawberry fruits, and has high commodity rate and high yield.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. The components of embodiments of the present invention may be arranged and designed in a wide variety of different configurations.

Powdery mildew may occur throughout the growth period of the strawberries. At present, aiming at strawberry powdery mildew, inorganic chemical medicines are mainly used, and a microbial agent is used for assisting singly, so that the strawberry powdery mildew is prevented and treated by root cultivation and seedling strengthening of various trace elements, fertilizers (leaf fertilizers or organic fertilizers) and the like.

The microbial agent is used for preventing and treating powdery mildew, and currently, a plurality of single-strain series are involved, and a few compound strains are used for preventing and treating the powdery mildew. The microbial agent for preventing and treating powdery mildew relates to the steps of preparation of the microbial agent, a using method of the microbial agent and a field test for preventing and treating the powdery mildew. The formula of the invention is preferably selected by carefully extracting, screening, domesticating and breeding various dominant strains from ecological environment, performing liquid and solid submerged fermentation and then expanded culture for commercialization, performing different component proportions on each single strain, and verifying the formula through field crop experiments, and the formula and the effects of preventing powdery mildew, increasing yield and the like are detailed below.

The composite bacterium for strawberries provided by the first aspect of the invention comprises bacteria, fungi and a carrier; wherein the mass ratio of the bacteria to the fungi to the carrier is 35-60: 3-4: 30-50;

wherein the bacteria include Bacillus subtilis, Bacillus laterosporus, Bacillus methylotrophicus, Rhodopseudomonas palustris and Bacillus cereus.

The method is used for relieving serious drug residues in soil and fruits and serious seedling root damage in the existing strawberry powdery mildew prevention and control technology; the fertilizer utilization rate is low, and soil hardening is serious; low yield, low commodity rate and poor quality. The compound bacteria for strawberries provided by the invention use microorganisms which are harmless to the environment, solve the problem of serious drug residue in soil and fruits, and relieve the damage of pesticides to seedling roots. Space and nutrients of pathogenic bacteria are preempted through the synergistic effect of bacteria and fungi in the microorganisms, and mass propagation is performed, so that hypha of powdery mildew pathogenic bacteria are deformed, and the growth of the pathogenic bacteria is inhibited. And the microorganisms secrete physiological active substances such as auxin, vitamins, amino acid and the like, so that the growth of plants is promoted, the using amount of the fertilizer is reduced, the fertilizer efficiency is improved, and the planting cost is reduced. The composite bacteria promote the propagation of beneficial bacteria in soil, balance soil acid and alkali, break soil hardening and improve strawberry yield.

Validamycin is an antibiotic secreted by microorganisms, has strong systemic property, is easily absorbed by and quickly conducted in thallus cells of strawberry powdery mildew pathogenic bacteria, interferes and inhibits the growth and development of the pathogenic bacteria cells, and thus has a treatment effect.

The bacillus subtilis belongs to bacillus, and the bacillus secretes antibacterial substances such as iturin, bacteriocin, fengycin, surfactant and the like in soil, inhibits growth of powdery mildew pathogenic bacteria, breaks and disintegrates hypha, dissolves cell walls of the pathogenic bacteria, causes perforation, deformity and the like of the pathogenic bacteria, and loses expansion and invasion capacity of the pathogenic bacteria.

The bacillus laterosporus is fixedly planted at the root of the strawberry, inhibits the propagation of powdery mildew pathogenic bacteria, promotes the growth of the root system of the plant, enhances the absorption capacity of the root system, can activate soil nutrients (nitrogen fixation, phosphorus and potassium dissolution), improves the yield of crops and improves the quality.

The bacillus methylotrophicus is propagated by utilizing nutrition and moisture on strawberry leaf surfaces, and antifungal active lipopeptide compounds generated by metabolism of the bacillus methylotrophicus have strong antibacterial activity on strawberry fungal diseases; the amino-peptidase substances are produced by the metabolism of the amino-peptidase substances, and simultaneously, the amino-peptidase substances are used as plant growth promoting bacteria to promote the growth of plants.

The rhodopseudomonas palustris secretion contains various bactericidal active substances, the growth and the propagation of pathogenic microorganisms are effectively controlled through competitive inhibition, and the secretion can induce the disease resistance of strawberry plants.

The bacillus cereus can improve the capability of removing oxygen generated in vivo by crops caused by pathogenic bacteria and adversity hazards through SOD enzyme in vivo, regulate the microbial environment of crop cells, maintain the normal physiological normal metabolism and biochemical environment of the cells, improve the stress resistance of the crops, promote the growth and improve the yield and the quality.

In a preferred embodiment of the invention, the mass ratio of bacteria, fungi and carrier is typically, but not limited to, 35: 3: 50. 35: 3: 30. 45, and (2) 45: 4: 30. 45, and (2) 45: 4: 50. 45, and (2) 45: 4: 40. 20: 1: 10. 30: 2: 25 or 15: 1: 10.

further, the mass ratio of the bacillus subtilis, the bacillus laterosporus, the bacillus methylotrophicus, the rhodopseudomonas palustris and the bacillus cereus in the bacteria is 60-100: 4-8: 4-6: 4-6: 2 to 3.

In a preferred embodiment of the invention, the mass ratio of Bacillus subtilis, Bacillus laterosporus, Bacillus methylotrophicus, Rhodopseudomonas palustris and Bacillus cereus is typically, but not limited to, 30: 2: 2: 2: 1. 40: 2: 2: 2: 1. 50: 2: 2: 2: 1. 100, and (2) a step of: 8: 6: 6: 3. 40: 4: 3: 3: 1 or 80: 8: 6: 6: 3.

further, the fungus comprises pythium oligandrum.

Pythium oligandrum can be colonized around strawberry roots, has direct or indirect inhibition or killing effect on powdery mildew pathogenic bacteria, can promote the absorption of phosphorus by strawberries, can increase the content of indoleacetic acid (IAA) in plants, promotes the growth of the plants, and induces the plants to generate defense reaction to obtain resistance.

Further, the carrier comprises potassium fulvate.

Preferably, the potassium fulvate is mineral potassium fulvate.

The mineral potassium fulvate can promote the formation of a soil aggregate structure, improve the soil structure and improve the water retention and air permeability of the soil. The nutrient effectiveness is improved, the physiological metabolism of strawberries can be stimulated, the emergence rate is improved, the division of the extreme meristematic cells of the root system is stimulated, and the water and nutrient absorption of crops is promoted.

Further, the effective viable count of the Bacillus subtilis is 1-5% of 1000 billion/g of validamycin 200, and in a preferred embodiment of the invention, the effective viable count of the Bacillus subtilis is typically, but not limited to, 1000 billion/g of 1% of validamycin 200, 1000 billion/g of 2% of validamycin 200, 1000 billion/g of 2.5% of validamycin 200, 1000 billion/g of 3% of validamycin 200, 1000 billion/g of 4% of validamycin 200, or 1000 billion/g of 5% of validamycin 200.

Further, the effective viable count of the Bacillus laterosporus is 200-1000 hundred million/g.

Preferably, the effective viable count of the bacillus methylotrophicus is 80-200 hundred million/g.

Preferably, the effective viable count of the rhodopseudomonas palustris is 80-200 hundred million/g.

Preferably, the effective viable count of the bacillus cereus is 80-200 hundred million/g.

Further, the effective viable count of the pythium oligandrum is 100-200 hundred million/g.

According to another aspect of the present invention, in the preparation method of the composite bacteria for strawberry of the first aspect, the bacteria, the fungi and the carrier are uniformly mixed to obtain the composite bacteria for strawberry.

According to the preparation method of the composite bacteria for the strawberries, provided by the invention, the preparation conditions are mild, the microorganisms are not damaged, and the activity of the microorganisms in the composite bacteria is ensured. The preparation method of the composite bacteria for the strawberries provided by the invention has mild operation conditions, ensures the activity of the bacteria and the effectiveness of the product, and is suitable for large-scale industrial production.

The application of the composite bacteria for strawberries provided by the third aspect of the invention in strawberry cultivation.

The application of the composite bacteria for strawberries in strawberry planting can be used for rooting and strengthening seedlings, improving the disease resistance of strawberry plants and improving the yield and quality of strawberries.

Further, the application is carried out by a method of furrow application and spray application.

The strawberry complex bacteria contain active microorganisms, and are buried in soil during application, and are applied in ditches 5-10cm below ground instead of spreading on the ground surface. The composite bacteria for strawberries cannot cause any damage to roots and fruits of strawberry plants, so that the composite bacteria for strawberries are close to roots of the strawberries to the maximum extent during furrow application and are in contact with the roots of the strawberries to the maximum extent, and the fertilizer effect can be fully exerted. When the composition is sprayed, the composition is sprayed on the back of the leaves in the morning or evening to prevent ultraviolet rays and kill microorganisms.

Some embodiments of the present invention will be described in detail below with reference to examples. The embodiments described below and the features of the embodiments can be combined with each other without conflict. The effective viable count of the single microbial inoculum used in the examples and the comparative examples is shown in the following table 1.

TABLE 1 effective viable bacteria count table of microbial inoculum raw materials

Microbial inoculum	Effective viable count
		Jinggang-bacillus subtilis	2.5% validamycin 200-1000 hundred million/g
Bacillus laterosporus	200-1000 hundred million/g
		Bacillus methylotrophicus	80-200 hundred million/g
Rhodopseudomonas palustris	80-200 hundred million/g
		Bacillus cereus	80-200 hundred million/g
Pythium oligandrum	100-200 hundred million/g

Example 1

In the present example, the bacteria in the composite bacteria for strawberry were bacillus subtilis, bacillus laterosporus, bacillus methylotrophicus, rhodopseudomonas palustris and bacillus cereus in a ratio of 40: 4: 3: 3: 1, in terms of mass ratio. Bacteria, pythium oligandrum and mineral potassium fulvate in the composite bacteria for the strawberries are mixed according to the ratio of 45: 4: 40 by mass.

Example 2

This example provides a composite bacterium for strawberry, which is different from example 1 in that bacillus subtilis, bacillus laterosporus, bacillus methylotrophicus, rhodopseudomonas palustris and bacillus cereus were cultured in the following manner of 40: 2: 2: 2: 1, and the rest raw materials and steps are the same as those in example 1, and are not described again.

Example 3

This example provides a composite bacterium for strawberry, which is different from example 1 in that bacillus subtilis, bacillus laterosporus, bacillus methylotrophicus, rhodopseudomonas palustris and bacillus cereus were cultured in a ratio of 50: 2: 2: 2: 1, and the rest raw materials and steps are the same as those in example 1, and are not described again.

Example 4

This example provides a composite bacterium for strawberry, which is different from example 1 in that bacillus subtilis, bacillus laterosporus, bacillus methylotrophicus, rhodopseudomonas palustris and bacillus cereus were mixed in a ratio of 30: 2: 2: 2: 1, and the rest raw materials and steps are the same as those in example 1, and are not described again.

Example 5

This example provides a composite bacterium for strawberry, which is different from example 1 in that bacillus subtilis, bacillus laterosporus, bacillus methylotrophicus, rhodopseudomonas palustris and bacillus cereus were cultured in the ratio of 100: 8: 6: 6: 3, and the rest of the raw materials and the steps are the same as those in the example 1, and are not described again.

Example 6

This example provides a composite bacterium for strawberry, which is different from example 1 in that bacillus subtilis, bacillus laterosporus, bacillus methylotrophicus, rhodopseudomonas palustris and bacillus cereus were cultured in the ratio of 80: 8: 6: 6: 3, and the rest of the raw materials and the steps are the same as those in the example 1, and are not described again.

Example 7

This example provides a composite bacterium for strawberry, which is different from example 1 in that bacillus subtilis, bacillus laterosporus, bacillus methylotrophicus, rhodopseudomonas palustris and bacillus cereus were cultured in the ratio of 100: 50: 50: 50: 1, and the rest raw materials and steps are the same as those in example 1, and are not described again.

Example 8

This example provides a complex bacterium for strawberry, which is different from example 1 in that the ratio of bacteria, pythium oligandrum and mineral potassium fulvate is 35: 3: 30, and the rest of the raw materials and the steps are the same as those in example 1, and are not described again.

Example 9

This example provides a complex bacterium for strawberry, which is different from example 1 in that the ratio of bacteria, pythium oligandrum and mineral potassium fulvate is 45: 4:30, and the rest of the raw materials and the steps are the same as those in example 1, and are not described again.

Example 10

This example provides a complex bacterium for strawberry, which is different from example 1 in that the ratio of bacteria, pythium oligandrum and mineral potassium fulvate is 35: 3: 50, and the rest of the raw materials and the steps are the same as those in example 1, and are not described again.

Example 11

This example provides a complex bacterium for strawberry, which is different from example 1 in that the ratio of bacteria, pythium oligandrum and mineral potassium fulvate was 20: 1: 10, and the rest of the raw materials and the steps are the same as those in example 1, and are not described again.

Example 12

This example provides a complex bacterium for strawberry, which is different from example 1 in that the ratio of bacteria, pythium oligandrum and mineral potassium fulvate was 30: 2: 25, and the rest of the raw materials and the steps are the same as those in example 1, and are not described again.

Example 13

This example provides a complex bacterium for strawberry, which is different from example 1 in that the ratio of bacteria, pythium oligandrum and mineral potassium fulvate is 15: 1: 10, and the rest of the raw materials and the steps are the same as those in example 1, and are not described again.

Comparative example 1

The comparative example provides a composite bacterium for strawberry, which only contains bacteria and pythium oligandrum, and the mass ratio of the bacteria to the pythium oligandrum is 45: 4, other raw materials and steps are the same as those of the example 1, and are not described again.

Comparative example 2

The comparative example provides a composite bacterium for strawberries, which is different from example 1 in that the composite bacterium only contains bacteria and mineral potassium fulvate, and the mass ratio of the bacteria to the mineral potassium fulvate is 9: other raw materials and steps are the same as those in example 1, and are not described again.

Comparative example 3

The comparative example provides a composite bacterium for strawberries, which is different from example 1 in that the composite bacterium only contains pythium oligandrum and mineral potassium fulvate, and the mass ratio of the pythium oligandrum to the mineral potassium fulvate is 1: 10, other raw materials and steps are the same as those of example 1, and are not described again.

Comparative example 4

The comparative example provides a composite bacterium for strawberry, which is different from example 1 in that bacteria, pythium oligandrum and mineral potassium fulvate are mixed according to the ratio of 5: 1: 5, the rest raw materials and steps are the same as those in example 1, and are not described again.

Comparative example 5

This comparative example provides a composite bacterium for strawberry, which is different from example 1 in that Bacillus subtilis, Bacillus laterosporus, Bacillus methylotrophicus and Rhodopseudomonas palustris were mixed in a ratio of 15: 1: 1: 1, and the rest of the raw materials and the steps are the same as those in example 1 and are not described again.

Comparative example 6

This comparative example provides a composite bacterium for strawberry, which is different from example 1 in that bacillus subtilis, bacillus laterosporus, bacillus methylotrophicus and bacillus cereus were mixed in a ratio of 30: 2: 2: 1, and uniformly mixing. The rest of the raw materials and steps are the same as those in example 1, and are not described again.

Test example 1

After the strawberry complex bacteria provided in examples 1 to 13 and comparative examples 1 to 6 were activated with liquid, a small amount of bacterial suspension was inoculated on a PDA medium at three symmetrical points, and each point was treated repeatedly 4 times. Inoculating pathogenic bacteria blocks with diameter of 5mm, which are obtained by punching with a puncher, in the center of the culture dish, wherein the pathogenic bacteria comprise fusarium graminearum, powdery mildew, botrytis cinerea, verticillium wilt, fusarium solani and apple rot pathogen, and culturing the treated culture dish in an incubator at 28 ℃. And (3) inoculating only pathogenic bacteria on the PDA culture medium of the blank group, and measuring the diameter of the colony of the experimental group when the control colony grows over the flat plate. The calculation mode of the bacteriostasis rate is shown as the following formula (1).

Bacteriostatic ratio (%) - (blank group colony diameter-treatment group colony diameter)/blank group colony diameter × 100% formula (1)

The statistical results of the bacteriostatic ratio are shown in table 2.

TABLE 2 bacteriostatic effect of the composite bacterial suspension on pathogenic bacteria

Test example 2

Strawberry test varieties: red face

A place: yunnan province Huizui county

Selecting strawberry stolon seedlings with consistent growth, transplanting the strawberry stolon seedlings into a field for planting, and covering films after survival. The strawberry compound bacteria provided in examples 1-13 and comparative examples 1-6 and the clear water of the blank group were respectively and uniformly drip-irrigated, and were continuously used for 3 times, and were used for the first time after the survival of the strawberry after the permanent planting, with an interval of 15d each time, and other management was the same as that of the conventional production.

Measuring leaves, plant height, crown diameter and stem thickness of the strawberry in the fruit mature period; and (5) counting the yield of each plant and the yield per mu in the picking period. The above measurement statistics are shown in table 3.

TABLE 3 Effect of Complex bacteria on strawberry plant growth

TABLE 4 influence of Complex bacteria on strawberry fruit quality

Test example 3

This test example tested the inhibitory effect of the composite bacteria for strawberry provided in examples 1 to 13 and comparative examples 1 to 6 on strawberry powdery mildew.

The strawberry variety to be tested is 'zhaoji', strawberry stolon seedlings with consistent growth are selected and transplanted in a field for planting, and the strawberry seedlings are covered with films after survival. The compound bacteria, chlorothalonil and clear water for strawberries provided in examples 1 to 13 and comparative examples 1 to 6 are used as blank groups to be sprayed conventionally, and are diluted by water and then uniformly sprayed on the front and back surfaces of leaves, wherein the compound bacteria, chlorothalonil and clear water are used for 3 times, the interval time of the first two times is 7 days, and the interval time of the 2 nd time and the 3 rd time is 10 days. Other medicaments are not used for preventing and treating diseases during the test period, and other management is the same as that of the conventional production.

Disease conditions are investigated before the spraying of the 1 st time, and disease conditions are investigated 6 days after the spraying of the 1 st time, 6 days after the spraying of the 2 nd time and 10 days after the spraying of the 3 rd time respectively. Sampling is carried out at 5 points on the diagonal, 5 plants are taken at each point, all fruits and leaves are investigated for each plant, and the classification is carried out according to the percentage of lesion area on each fruit to the whole fruit. The diseases were classified as follows.

Level 0: the fruit is disease-free;

level 1: the area of the lesion spots accounts for less than 5% of the whole fruit surface;

and 3, level: the lesion area accounts for 6 to 15 percent of the whole fruit area;

and 5, stage: the lesion area accounts for 16 to 25 percent of the whole fruit area;

and 7, stage: the lesion area accounts for 26-50% of the whole fruit area;

and 9, stage: the lesion area accounts for more than 50% of the whole fruit area

The disease index and the preventive and therapeutic effects are calculated by the following formulas (2) and (3).

Disease index (%) ═ Σ (number of diseased fruits at each stage × relative disease stage number)/(total number of investigated fruits × highest disease stage) × 100 formula (2)

The control effect (%) (1-CK 0 XPT 1/PT0 XPT 1) x 100 formula (3)

CK0 is disease index before spraying for blank group; CK1 is disease index of the blank control group after spraying; PT0 is disease index before spraying for treatment group; PT1 is the index of disease after spraying in the treatment area.

TABLE 5 preventive and therapeutic effects of composite bacteria on powdery mildew

As can be seen from tables 3 and 4, the composite bacteria for strawberries provided by the invention have different degrees of promoting effects on the growth of crops, and the contents of Vc, soluble solid matters, soluble sugar, hardness and commercial fruit rate are all higher than those of the blank group.

As can be seen from table 5, after the composite bacteria for strawberries provided by the present invention are sprayed on the leaf surfaces of strawberries at different times, and sampled and analyzed for a period of time, the composite bacteria for strawberries and the pesticide chlorothalonil provided in examples 1 to 13 can both well prevent and treat powdery mildew, the control efficiency of the composite bacteria for strawberries reaches 80.7%, the control effect of example 1 is the best, but the control efficiency is always lower than that of the chlorothalonil, and the difference is within an acceptable range.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.