阅读说明：本技术 一种多温区的光电微生物检测仪器 (Photoelectric microorganism detection instrument with multiple temperature zones ) 是由 钟永捷 韦红 孟天 于 2021-01-26 设计创作，主要内容包括：本发明涉及一种多温区的光电微生物检测仪器,包括：箱体,箱体的底部固定设有固定底座,箱体的顶部设有开口,箱体内部固定安装有内箱体,内箱体上分布有多个温度区域；盖体,盖体扣合在箱体的开口上,盖体与箱体铰接；多个温度控制装置,温度控制装置固定安装在温度区域内,温度控制装置包括金属导体、PTC加热片、温度传感器和半导体制冷片,PTC加热片和半导体制冷片均贴附在金属导体的底部,金属导体上插设有多个试管,PTC加热片用于提升试管的温度,半导体制冷片用于降低试管的温度,温度传感器用于测量试管的温度,每个温度控制装置上设有与其相对应的控制开关。(The invention relates to a photoelectric microorganism detecting instrument with multiple temperature zones, which comprises: the temperature control box comprises a box body, wherein a fixed base is fixedly arranged at the bottom of the box body, an opening is formed in the top of the box body, an inner box body is fixedly arranged in the box body, and a plurality of temperature areas are distributed on the inner box body; the cover body is buckled on the opening of the box body and is hinged with the box body; a plurality of temperature control device, temperature control device fixed mounting is in the temperature zone, temperature control device includes metallic conductor, the PTC heating plate, temperature sensor and semiconductor refrigeration piece, PTC heating plate and semiconductor refrigeration piece are all attached in metallic conductor's bottom, it is equipped with a plurality of test tubes to insert on the metallic conductor, the PTC heating plate is used for promoting the temperature of test tube, semiconductor refrigeration piece is used for reducing the temperature of test tube, temperature sensor is used for measuring the temperature of test tube, be equipped with on every temperature control device rather than corresponding control switch.)

1. A multi-temperature zone photoelectric microorganism detection instrument is characterized by comprising:

the temperature control box comprises a box body, wherein a fixed base is fixedly arranged at the bottom of the box body, an opening is formed in the top of the box body, an inner box body is fixedly arranged in the box body, and a plurality of temperature areas are distributed on the inner box body;

the cover body is buckled on the opening of the box body and is hinged with the box body;

a plurality of temperature control device, temperature control device fixed mounting is in the temperature zone, temperature control device includes metallic conductor, PTC heating plate, temperature sensor and semiconductor refrigeration piece, PTC heating plate and semiconductor refrigeration piece are all attached metallic conductor's bottom, it is equipped with a plurality of test tubes to insert on the metallic conductor, the PTC heating plate is used for promoting the temperature of test tube, semiconductor refrigeration piece is used for reducing the temperature of test tube, temperature sensor is used for measuring the temperature of test tube, every be equipped with on the temperature control device rather than corresponding control switch.

2. The optoelectronic microorganism detection instrument of claim 1, wherein the plurality of temperature zones are separated by a partition.

3. The apparatus according to claim 1, wherein the metal conductor comprises a first conductive block and a second conductive block, and a plurality of first detecting holes and second detecting holes are distributed on the first conductive block and the second conductive block.

4. The apparatus according to claim 3, wherein the metal conductor further comprises a bottom plate, a cover plate, a left side plate, a right side plate and a support plate, the left side plate is fixedly connected to the left side of the bottom plate, the right side plate is fixedly connected to the right side of the bottom plate, the support plate is fixedly mounted at the middle of the bottom plate, the cover plate is fixedly mounted at the top ends of the left side plate, the right side plate and the support plate, the first conductive block is located between the left side plate and the support plate, and the second conductive block is located between the support plate and the right side plate.

5. The apparatus according to claim 4, wherein one side of the first conductive block is fixedly connected to the left side plate, the bottom of the first conductive block is fixedly connected to the bottom plate, the top of the first conductive block abuts against the cover plate, and a first gap is formed between the other side of the first conductive block and the support plate; one side of the second conduction block is fixedly connected with the supporting plate, the bottom of the second conduction block is fixedly connected with the bottom plate, the top of the second conduction block is abutted to the cover plate, and a second gap is formed between the other side of the second conduction block and the right side plate.

6. The photoelectric microorganism detection instrument of claim 5, wherein the first conductive block is provided with a plurality of first vent holes, one end of each first vent hole is communicated with the first detection hole, and the other end of each first vent hole is communicated with the first gap; and a plurality of second ventilation holes are formed in the second conduction block, one end of each second ventilation hole is communicated with the second detection hole, and the other end of each second ventilation hole is communicated with the second gap.

7. The optoelectronic microorganism detecting apparatus of claim 3, wherein a first supporting frame is disposed at the bottom of the first detecting hole of the first conductive block, and the first supporting frame is used for supporting the test tube; the bottom in the second inspection hole of second conduction piece is equipped with the second support frame, the second support frame is used for supporting the test tube.

8. The apparatus according to claim 7, wherein the first supporting frame comprises a first left hollow cylinder and a first right hollow cylinder, the first left hollow cylinder is disposed opposite to the first right hollow cylinder, a light source is disposed in the first left hollow cylinder, and a light sensor is disposed in the first right hollow cylinder; the second support frame includes hollow cylinder in the left side of the second and the hollow cylinder in the right side of the second, the hollow cylinder in the left side of the second with the hollow cylinder in the right side of the second sets up relatively, be equipped with the light source in the hollow cylinder in the left side of the second, be equipped with light sensor in the hollow cylinder in the right side of the second.

9. The apparatus according to claim 8, wherein the first left hollow cylinder, the first right hollow cylinder, the second left hollow cylinder and the second right hollow cylinder are all fan-shaped cylinders.

10. The optoelectronic microorganism detecting instrument of claim 9, wherein the test tube is in a cone shape with a large opening and a small bottom, one end of the test tube is screwed with a test tube cover, the bottom of the test tube is provided with an opening, the top of the opening is in a triangular shape matched with the fan-shaped cylinder, and the bottom of the opening is larger than the width of the fan-shaped cylinder.

Technical Field

The invention relates to a microorganism detection instrument, in particular to a photoelectric microorganism detection instrument with multiple temperature zones.

Background

The photoelectric microorganism detection instrument is used for quickly and accurately detecting pathogenic microorganisms based on an optical detection technology, and can be applied to the fields of food safety, environmental monitoring, medical supplies, cosmetics, agriculture, forestry, animal husbandry, health and epidemic prevention and the like, which relate to the diagnosis and monitoring of microorganisms. The photoelectric microorganism detector sold in the market at present mainly detects microorganisms which can be cultured at a single temperature (room temperature), cannot control the temperature condition of culture according to experiment or detection requirements, and has poor detection effect on thermophilic bacteria, so that the photoelectric microorganism detector has large limitation in practical application and cannot meet different requirements of users.

The Chinese patent CN201910698218.1 discloses a multi-temperature zone microorganism detection instrument, but the instrument has many defects: 1. the cooling by using the fan and the radiating fins is slow, and when some microorganisms are lower than the current room temperature, the fan and the radiating fins are obviously insufficient, and the noise is extremely high. 2. Adopt drawer type structure can cause when the pull to rock at the detection test tube, lead to the detection curve to produce the skew, and drawer type structure damages easily, and is not as stable structure. 3. The instrument only has 32 channels and two temperature zones, and the requirement of more times is not met.

Disclosure of Invention

The invention mainly aims to provide a photoelectric microorganism detection instrument with multiple temperature zones, which realizes controllable temperature of sample detection, meets high-flux detection at a single temperature, can also ensure detection under multiple temperature conditions, realizes rapid cooling and stable structure, and expands the functions and application range of the instrument.

In order to accomplish the above object, the present invention provides a multi-temperature zone photoelectric microorganism detection apparatus, comprising: the temperature control box comprises a box body, wherein a fixed base is fixedly arranged at the bottom of the box body, an opening is formed in the top of the box body, an inner box body is fixedly arranged in the box body, and a plurality of temperature areas are distributed on the inner box body; the cover body is buckled on the opening of the box body and is hinged with the box body; a plurality of temperature control device, temperature control device fixed mounting is in the temperature zone, temperature control device includes metallic conductor, the PTC heating plate, temperature sensor and semiconductor refrigeration piece, PTC heating plate and semiconductor refrigeration piece are all attached in metallic conductor's bottom, it is equipped with a plurality of test tubes to insert on the metallic conductor, the PTC heating plate is used for promoting the temperature of test tube, semiconductor refrigeration piece is used for reducing the temperature of test tube, temperature sensor is used for measuring the temperature of test tube, be equipped with on every temperature control device rather than corresponding control switch.

Preferably, the plurality of temperature zones are isolated from each other by a partition.

Preferably, the metal conductor includes a first conductive block and a second conductive block, and a plurality of first detection holes and second detection holes are distributed on the first conductive block and the second conductive block.

Further preferably, the metal conductor further comprises a bottom plate, a cover plate, a left side plate, a right side plate and a support plate, the left side plate is fixedly connected with the left side of the bottom plate, the right side plate is fixedly connected with the right side of the bottom plate, the support plate is fixedly arranged in the middle of the bottom plate, the cover plate is fixedly arranged on the top ends of the left side plate, the right side plate and the support plate, the first conducting block is arranged between the left side plate and the support plate, and the second conducting block is arranged between the support plate and the right side plate.

Preferably, one side of the first conducting block is fixedly connected with the left side plate, the bottom of the first conducting block is fixedly connected with the bottom plate, the top of the first conducting block is abutted against the cover plate, and a first gap is formed between the other side of the first conducting block and the supporting plate; one side of the second conduction block is fixedly connected with the supporting plate, the bottom of the second conduction block is fixedly connected with the bottom plate, the top of the second conduction block is abutted to the cover plate, and a second gap is formed between the other side of the second conduction block and the right side plate.

Preferably, the first conductive block is provided with a plurality of first vent holes, one end of each first vent hole is communicated with the first detection hole, and the other end of each first vent hole is communicated with the first gap; and a plurality of second ventilation holes are formed in the second conduction block, one end of each second ventilation hole is communicated with the second detection hole, and the other end of each second ventilation hole is communicated with the second gap.

Further preferably, a first support frame is arranged at the bottom of the first detection hole of the first conduction block and used for supporting the test tube; the bottom in the second inspection hole of second conduction piece is equipped with the second support frame, and the second support frame is used for supporting the test tube.

Preferably, the first support frame comprises a first left hollow cylinder and a first right hollow cylinder, the first left hollow cylinder and the first right hollow cylinder are arranged oppositely, a light source is arranged in the first left hollow cylinder, and an optical sensor is arranged in the first right hollow cylinder; the second support frame comprises a second left hollow cylinder and a second right hollow cylinder, the second left hollow cylinder and the second right hollow cylinder are arranged oppositely, a light source is arranged in the second left hollow cylinder, and an optical sensor is arranged in the second right hollow cylinder.

Further preferably, the first left hollow cylinder, the first right hollow cylinder, the second left hollow cylinder and the second right hollow cylinder are all fan-shaped cylinders.

Still further preferably, the test tube is big end bottom coniform, and the one end spiro union of test tube has the test tube lid, and the bottom of test tube is equipped with the opening, and open-ended top is the triangle-shaped form that matches with fan-shaped cylinder, and open-ended bottom is greater than fan-shaped cylinder's width.

The invention has the beneficial effects that: the inner box body is divided into a plurality of independent temperature areas, the temperature control devices are arranged in the temperature areas, and each temperature control device is provided with an independent control switch so as to realize the control of different temperature areas. In addition, in the invention, the PTC heating sheet is used for heating the metal conductor to realize the temperature rise control of the test tube, and the semiconductor refrigerating sheet is used for reducing the temperature of the metal conductor, thereby realizing the temperature reduction control of the test tube and quickly realizing the temperature rise and the temperature reduction of the test tube.

Drawings

FIG. 1 is a top view of an optoelectronic microorganism detection apparatus of the present invention;

FIG. 2 is a schematic structural diagram of an inner case of the photoelectric microorganism detection apparatus of the present invention;

FIG. 3 is a top view of the inner case of the photoelectric microorganism detection apparatus of the present invention;

FIG. 4 is a left side view (one) of a cuvette of the optoelectronic microorganism detection apparatus of the present invention;

FIG. 5 is a left side view (II) of the cuvette of the photoelectric microorganism detecting apparatus of the present invention.

Description of the reference numerals

100. A box body; 110. a cover body; 120. a display screen; 130. a switch button;

200. an inner box body; 210. a partition plate; 220. a cover plate; 221. a detection hole;

230. a second conductive block; 240. a first conductive block; 260. a support frame body;

261. a base plate; 262. a left side plate; 263. a right side plate; 264. a support plate;

250. a first support frame; 270. a second support frame; 300. test tubes.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.

As shown in fig. 1 and fig. 2, the present embodiment provides a multi-temperature-zone photoelectric microorganism detection apparatus, which includes a box 100, wherein a fixing base (not shown in the figure) is fixed at the bottom of the box 100, preferably, the fixing base is a metal bracket, so that the test curve offset caused by the test tube shaking due to the instrument shaking can be avoided. The top of the box 100 is provided with an opening, an inner box 200 is fixedly installed inside the box 100, and a plurality of temperature areas are distributed on the inner box 200, preferably, the temperature areas are isolated by a partition 210. The cover body 110 is buckled on the opening of the box body 100, the cover body 110 is hinged with the box body 100, preferably, the cover body 110 is made of lightproof materials, the other end of the cover body 110 is connected with the box body in a clamped mode, the service life of the structure is prolonged by the aid of the cover-turning design with the bayonet and the fixing base, and the characteristic that drawer type is prone to being broken due to frequent drawing is reduced. The temperature control device is fixedly installed in the temperature area and comprises a metal conductor, a PTC heating piece (not shown in the figure), a temperature sensor (not shown in the figure) and a semiconductor refrigerating piece (not shown in the figure), wherein the PTC heating piece and the semiconductor refrigerating piece are both attached to the bottom of the metal conductor, and in addition, the body positions of the PCT heating piece and the semiconductor refrigerating piece can be adjusted according to the actual situation and are not fixed. Insert on the metallic conductor and be equipped with a plurality of test tubes 300, the PTC heating plate is used for promoting the temperature of test tube 300, and the semiconductor refrigeration piece is used for reducing the temperature of test tube 300, and temperature sensor is used for measuring the temperature of test tube 300, is equipped with rather than corresponding control switch on every temperature control device. A display screen 120 and a switch button 130 are also provided on the case 100.

In addition, in the present embodiment, the present photoelectric microorganism detector with 16 detection channels is expanded into 64 channels, and the number of the channels is increased from a single temperature zone to 4 temperature zones, and each zone has 16 measurement channels. The instrument is added with 4 temperature control parts, and each temperature area is controlled by a separate control switch, preferably a power switch MOS tube. The temperature control adopts a PID algorithm, the reading of the temperature sensor is realized through GPIO, the temperature sensor is fed back to the main control console, the switching action of the power switch MOS tube is controlled, the control of the PTC heating piece and the semiconductor refrigerating piece is realized, the temperature control of the incubation environment is realized, and the semiconductor refrigerating piece adopts the switch control of a general triode. In this embodiment, each temperature zone of the 64-channel microbial detector is cooled by using a semiconductor refrigeration sheet, which is more obvious than the cooling by using a fan, so that the waiting time of a user after measuring a strain is reduced, in particular, for some strains such as mold, the culture temperature is 28 ℃, which may be lower than the room temperature in some seasons in some regions, so that the cooling effect of the fan is much worse, and the temperature of the test tube can be kept constant by using the semiconductor refrigeration sheet and the PID algorithm. The instrument is designed with 64 channels, the function and application range of the instrument are expanded, the strains measured simultaneously are greatly increased by four temperature zones, on the basis, the size of the instrument is not increased greatly, the transverse size of a test tube is reduced, the size of the 64-channel instrument is still the normal instrument size under the condition of not influencing the detection result, the length of the whole instrument is 39.5cm, the width is 38.3cm, the height is 15cm, the occupied area is not obviously increased, the transportation difficulty is not increased, and the instrument is reasonable in size. The photoelectric microorganism detector in the embodiment adopts an android display curve and uses a screen cutting technology, 16 channel curves are on the same interface, four temperature zones respectively correspond to four different interfaces, sliding left and right is carried out to switch and view, so that sense experience that dense, hemp and uncomfortable feeling are caused by the fact that all the channels are in the same interface is avoided, and if the details are wanted to be viewed, a certain channel can be clicked to view a measurement curve of a single channel. The during operation, place the hole through DS18B20 temperature sensor to the reagent and carry out the collection input of temperature and give the controller, the controller comes power switch MOS pipe and semiconductor refrigeration piece to control according to the temperature value of gathering, when the temperature is higher than the set value, start the cooling of semiconductor refrigeration piece module, make metallic conductor's temperature reduce, thereby make test tube temperature reduce, when the temperature is less than the set value, start PTC heating plate and heat up, make metallic conductor's temperature rise, thereby make test tube temperature rise.

In this embodiment, as shown in fig. 2, the metal conductor includes a first conductive block 240 and a second conductive block 230, and a plurality of first detection holes (not shown) and second detection holes (not shown) are distributed on the first conductive block 240 and the second conductive block 230, and in this embodiment, each of the first detection holes and the second detection holes is 8. In addition, the metal conductor further includes a supporting frame 260 and a cover plate 220, the cover plate 220 is covered on the supporting frame 260, and the cover plate 220 is provided with a plurality of detection holes 221. Specifically, the supporting frame 260 includes a bottom plate 261, a left side plate 262, a right side plate 263 and a supporting plate 264, the supporting plate is fixedly installed in the middle of the bottom plate, the left side plate 262 is fixedly connected with the left side of the bottom plate 261, one side of the first conducting block 240 is fixedly connected with the left side plate 262, the bottom of the first conducting block 240 is fixedly connected with the bottom plate 261, the top of the first conducting block 240 abuts against the cover plate 220, a first detecting hole on the first conducting block 240 is matched with one row of detecting holes 221 on the cover plate 220 so as to insert the test tube 300, and a first gap is formed between the other side of the first conducting block 240 and the supporting plate 264; the right side plate 263 is fixedly connected to the right side of the bottom plate 261, one side of the second conductive block 230 is fixedly connected to the supporting plate 264, the bottom of the second conductive block 230 is fixedly connected to the bottom plate 261, the top of the second conductive block 230 abuts against the cover plate 220, and the second detecting hole of the second conductive block 230 is matched with the other row of detecting holes 221 of the cover plate 220, so that the test tube 300 can be inserted into the second detecting hole, and a second gap is formed between the other side of the second conductive block 230 and the right side plate 263. The cover plate 220 is fixedly mounted on top ends of the left side plate 262, the right side plate 263 and the support plate 264. In addition, in this embodiment, a plurality of first vent holes (not shown in the figure) are disposed on the first conductive block 240, one end of each first vent hole is communicated with the first detection hole, and the other end of each first vent hole is communicated with the first gap, that is, the first vent holes are used for communicating the first detection hole and the first gap; the second conductive block 230 is provided with a plurality of second vents (not shown), one end of each second vent is communicated with the second detecting hole, and the other end of each second vent is communicated with the second gap, that is, the second vents are used for communicating the second detecting hole with the second gap. In the in-service use process, after test tube 300 inserts the inspection hole, it is possible to make the bottom of test tube 300 and metal form sealed form to the end between, and the bottom of first inspection hole or/and second inspection hole forms confined space promptly, and because ambient temperature meets the end, and the microorganism that detects needs higher temperature, then in PTC heating plate in-process for the gas expansion in the confined space, it is possible to make the test tube produce and rock, thereby influence the testing result, consequently, utilize first ventilation hole and second ventilation hole to exhaust.

In the present embodiment, as shown in fig. 3, a first supporting frame 250 is disposed at the bottom of the first detecting hole of the first conducting block 240, and the first supporting frame 250 is used for supporting the test tube 300; the bottom of the second detection hole of the second guide block 230 is provided with a second support bracket 270, and the second support bracket 270 is used for supporting the test tube 300. Specifically, the first support frame 250 includes a first left hollow cylinder and a first right hollow cylinder, the first left hollow cylinder and the first right hollow cylinder are disposed opposite to each other, a light source (not shown in the figure) is disposed in the first left hollow cylinder, and an optical sensor (not shown in the figure) is disposed in the first right hollow cylinder; the second support frame 270 includes a second left hollow cylinder and a second right hollow cylinder, the second left hollow cylinder and the second right hollow cylinder are disposed oppositely, a light source (not shown in the figure) is disposed in the second left hollow cylinder, and an optical sensor (not shown in the figure) is disposed in the second right hollow cylinder.

In this embodiment, as shown in fig. 4, the test tube 300 is a cone with a large opening and a small bottom, and the test tube 300 is provided with a test tube cover (not shown in the figure) which is screwed with the test tube 300, wherein the test tube cover is a black screw cap, and the whole test tube 300 tube body adopts a large-bottom mode, so that the instrument can be placed in the test tube, and the test tube cover can completely cover the opening, thus solving the problem that the instrument cover can be transparent when being opened. The bottom of test tube 300 is equipped with the opening, and the opening card is established on the support frame, prevents that test tube 300 from trembling. In addition, in the use, owing to use the columniform support frame, make the projection of the bottom of test tube 300 at the horizontal plane be rectangle (as shown in fig. 4), when taking off test tube 300, the opening of test tube 300 slightly is less than the diameter of support frame, make test tube 300 in the in-process of extracting the support frame, the phenomenon that can produce the shake, this kind influences the observation of little biological fluid in test tube 300, consequently, as shown in fig. 5, the open-ended top is the triangle-shaped for the projection on the horizontal plane, the open-ended bottom is greater than the width of fan-shaped cylinder, and for the cooperation installation test tube 300, first left hollow cylinder, first right hollow cylinder, second left hollow cylinder and second right hollow cylinder are fan-shaped cylinder, so that the bottom of test tube 300 is placed on the support frame.

It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.