阅读说明：本技术 包括重构固氮微生物的农业组合物 (Agricultural compositions comprising reconstituted nitrogen-fixing microorganisms ) 是由 M·赖辛格 E·桑德斯 R·布罗格利 K·特米 于 2019-06-26 设计创作，主要内容包括：本发明提供非属间重塑微生物,其能够固定大气氮且以靶向、高效和环境可持续的方式将此类氮输送到植物。利用所教示的微生物产品将使得农民能够实现更高产和可预测的作物产量,而不存在与传统的合成氮肥相关的营养物降解、浸出或有毒径流。本文所教示的所述重塑微生物能够与领先的农业化学物质和优良种质组合。此外,本发明提供包括重塑微生物的种子处理剂。(The present invention provides non-intergeneric remodelling microorganisms capable of immobilising atmospheric nitrogen and delivering such nitrogen to plants in a targeted, efficient and environmentally sustainable manner. Utilizing the taught microbial products will enable farmers to achieve higher and predictable crop yields without nutrient degradation, leaching or toxic runoff associated with traditional synthetic nitrogen fertilizers. The remodeled microorganisms taught herein are capable of combining with leading agrochemicals and superior germplasm. In addition, the present invention provides a seed treatment comprising a remodeling microorganism.)

1. A seed treatment composition comprising:

a. a plurality of non-intergeneric remodeling bacteria having at least about 1.0 x 10 per unit plant root tissue⁴An average colonization capacity of at least about 1X 10 per hour per gram fresh weight of root tissue of the plant^-17mmol N/fixed N of bacterial cells; and

b. at least one pesticide.

2. The seed treatment composition according to claim 1, wherein the pesticide is a fungicide.

3. The seed treatment composition according to claim 1, wherein the pesticide is a fungicide selected from the group consisting of: fludioxonil, metalaxyl-M, azoxystrobin, thiabendazole, ipconazole, tebuconazole, prothioconazole and combinations thereof.

4. The seed treatment composition according to claim 1, wherein the pesticide is an insecticide.

5. The seed treatment composition according to claim 1, wherein the pesticide is a neonicotinoid pesticide.

6. The seed treatment composition according to claim 1, wherein the pesticide is an insecticide selected from the group consisting of: imidacloprid, clothianidin, thiamethoxam, chlorantraniliprole, and combinations thereof.

7. The seed treatment composition according to claim 1, wherein the pesticide is a combination of a fungicide and an insecticide.

8. The seed treatment composition according to claim 1, wherein the pesticide is a nematicide.

9. The seed treatment composition according to claim 1, wherein the pesticide is a herbicide.

10. The seed treatment composition according to claim 1, wherein the pesticide is a pesticide selected from those in table 13.

11. The seed treatment composition of claim 1, wherein the non-intergeneric remodelling bacterium and pesticide exhibit a synergistic effect.

12. The seed treatment composition of claim 1, wherein the seed treatment is disposed onto a seed.

13. The seed treatment composition according to claim 1, wherein the seed treatment is provided to a seed from the family Poaceae.

14. The seed treatment composition of claim 1, wherein the seed treatment is disposed onto a cereal seed.

15. The seed treatment composition of claim 1, wherein the seed treatment is disposed on a corn, rice, wheat, barley, sorghum, millet, oat, rye, or triticale seed.

16. The seed treatment composition of claim 1, wherein the seed treatment is disposed onto corn seed.

17. The seed treatment composition of claim 1, wherein the seed treatment is disposed onto a genetically modified corn seed.

18. The seed treatment composition of claim 1, wherein the seed treatment is disposed onto a genetically modified corn seed, wherein the corn comprises a herbicide tolerant trait.

19. The seed treatment composition of claim 1, wherein the seed treatment is disposed onto a genetically modified corn seed, wherein the corn comprises an insect-resistant trait.

20. The seed treatment composition of claim 1, wherein the seed treatment is disposed onto a genetically modified corn seed, wherein the corn comprises herbicide tolerance characteristics and insect resistance traits.

21. The seed treatment composition of claim 1, wherein the seed treatment is disposed onto a genetically modified corn seed, wherein the corn comprises the traits listed in table 19.

22. The seed treatment composition of claim 1, wherein the seed treatment is disposed onto non-genetically modified corn seed.

23. The seed treatment composition of claim 1, wherein the seed treatment is disposed onto sweet corn, hard corn, pop corn, dent corn, pod corn, or mealy corn.

24. The seed treatment composition of claim 1, wherein the plurality of non-intergeneric remodeling bacteria produces 1% or more of the fixed nitrogen in a plant exposed thereto.

25. The seed treatment composition of claim 1, wherein the non-intergeneric remodeling bacterium is capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen.

26. The seed treatment composition of claim 1, wherein each member of the plurality of non-intergeneric remodelling bacteria comprises at least one genetic variation in at least one gene or non-coding polynucleotide introduced into a nitrogen-fixing or assimilating genetic regulatory network.

27. The seed treatment composition of claim 1, wherein each member of the plurality of non-intergeneric remodelling bacteria comprises an introduced control sequence of at least one gene operably linked to a nitrogen fixation or assimilation genetic regulatory network.

28. The seed treatment composition of claim 1, wherein each member of the plurality of non-intergeneric remodelling bacteria comprises a heterologous promoter operably linked to at least one gene of a nitrogen fixation or assimilation genetic regulatory network.

29. The seed treatment composition of claim 1, wherein each member of the plurality of non-intergeneric remodelling bacteria includes at least one genetic variation introduced into a member selected from the group consisting of: nifA, nifL, ntrB, ntrC, a polynucleotide encoding glutamine synthetase, glnA, glnB, glnK, drat, amtB, a polynucleotide encoding glutaminase, glnD, glnE, nifJ, nifH, nifD, nifK, nifY, nifE, nifN, nifU, nifS, nifV, nifW, nifZ, nifM, nifF, nifB, nifQ, a gene associated with nitrogenase biosynthesis, or a combination thereof.

30. The seed treatment composition of claim 1, wherein each member of the plurality of non-intergeneric remodelling bacteria comprises at least one genetic variation among at least one gene or non-coding polynucleotide that introduces a nitrogen-fixing or assimilating genetic regulatory network that produces one or more of: increased expression or activity of NifA or glutaminase; reduced expression or activity of NifL, NtrB, glutamine synthetase, GlnB, GlnK, DraT, AmtB; reduced desadenoadenylyl activity of GlnE; or reduced detruridine-removing activity of GlnD.

31. The seed treatment composition of claim 1, wherein each member of the plurality of non-intergeneric remodelling bacteria comprises a mutant nifL gene that has been altered to include a heterologous promoter inserted into the nifL gene.

32. The seed treatment composition of claim 1, wherein each member of the plurality of non-intergeneric remodelling bacteria comprises a mutant glnE gene that produces a truncated GlnE protein lacking a desadenoadenylyl AR domain.

33. The seed treatment composition of claim 1, wherein each member of the plurality of non-intergeneric remodelling bacteria comprises a mutant amtB gene that results in a lack of expression of the amtB gene.

34. The seed treatment composition of claim 1, wherein each member of the plurality of non-intergeneric remodeling bacteria comprises at least one of: a mutant nifL gene that has been altered to include a heterologous promoter inserted into the nifL gene; a mutant glnE gene that produces a truncated GlnE protein lacking a desadenosyl AR domain; a mutant amtB gene, which results in a lack of expression of said amtB gene; and combinations thereof.

35. The seed treatment composition of claim 1, wherein each member of the plurality of non-intergeneric remodeling bacteria comprises a mutant nifL gene that has been altered to include a heterologous promoter inserted into the nifL gene and a mutant glnE gene that produces a truncated glnE protein lacking a desadenosyl AR domain.

36. The seed treatment composition of claim 1, wherein each member of the plurality of non-intergeneric remodelling bacteria comprises a mutant nifL gene and a mutant glnE gene and a mutant amtB gene, the mutant nifL gene having been altered to include a heterologous promoter inserted into the nifL gene; the mutant glnE gene produces a truncated GlnE protein lacking a desadenosyl AR domain; the mutant amtB gene results in a lack of expression of the amtB gene.

37. The seed treatment composition of claim 1, wherein the plurality of non-intergeneric remodeled bacteria is present at about 1 x 10 per seed⁵To about 1X 10⁷The concentration of cfu is present.

38. The seed treatment composition of claim 1, wherein the plurality of non-intergeneric remodeling bacteria comprises at least two different bacterial species.

39. The seed treatment composition of claim 1, wherein the plurality of non-intergeneric remodeling bacteria comprises at least two different strains of the same bacterial species.

40. The seed treatment composition of claim 1, wherein the plurality of non-intergeneric remodeling bacteria comprises a bacteria selected from the group consisting of: rahnella aquaticus, Klebsiella variicola, Achromobacter mentarius, Achromobacter marxianus, Microbacterium muralis, Kluyveromyces intermedius, Saccharomycosis pseudosaccharaceae, Enterobacter, Azospirillum lipolytica, Coxsackia saccharalis, and combinations thereof.

41. The seed treatment composition of claim 1, wherein the plurality of non-intergeneric remodeling bacteria are endogenous, epiphytic, or rhizospheric.

42. The seed treatment composition of claim 1, wherein the plurality of non-intergeneric remodeling bacteria comprises a bacteria selected from the group consisting of: the bacteria deposited with NCMA 201701002, the bacteria deposited with NCMA 201708004, the bacteria deposited with NCMA 201708003, the bacteria deposited with NCMA 201708002, the bacteria deposited with NCMA 201712001, the bacteria deposited with NCMA 201712002, and combinations thereof.

43. The seed treatment composition of claim 1, wherein the plurality of non-intergeneric remodeling bacteria comprises bacteria comprising a nucleic acid sequence having at least about 90% sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO 177-260, 296-303.

44. The seed treatment composition of claim 1, wherein the plurality of non-intergeneric remodeling bacteria comprises bacteria comprising a nucleic acid sequence having at least about 95% sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO 177-260, 296-303.

45. The seed treatment composition of claim 1, wherein the plurality of non-intergeneric remodeling bacteria comprises bacteria comprising a nucleic acid sequence having at least about 99% sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO 177-260, 296-303.

46. The seed treatment composition of claim 1, wherein the plurality of non-intergeneric remodeling bacteria comprises bacteria comprising nucleic acid sequences selected from the group consisting of SEQ ID NO:177-260, 296-303.