阅读说明：本技术 一种畜禽用饲料添加剂、其制备方法及应用 (Feed additive for livestock and poultry, preparation method and application thereof ) 是由 刘春海 武瑞 李金戈 于 2020-12-07 设计创作，主要内容包括：本发明公开了一种畜禽用饲料添加剂、其制备方法及应用,属于动物用饲料添加剂技术领域。所述畜禽用饲料添加剂,由包衣的植物精油组合物和包衣的单宁酸制成,其中,包衣的植物精油组合物由吸附后的植物精油组合物和包衣载体制成；吸附后的植物精油组合物由植物精油组合物和吸附剂制成；植物精油组合物由香芹酚、百里香酚和肉桂醛制成；包衣的单宁酸由单宁酸和包衣载体制成。本发明还公开了上述畜禽用饲料添加剂的制备方法及应用。本发明的畜禽用饲料添加剂,对畜禽动物机体有明显的改善和治疗效果,能满足畜禽动物的生长需求,促进畜禽动物的生长速度,还能提高畜禽动物的免疫力,防治腹泻,且长期使用不会产生耐药性、药物残留和毒副作用。(The invention discloses a feed additive for livestock and poultry, a preparation method and application thereof, and belongs to the technical field of feed additives for animals. The feed additive for livestock and poultry is prepared from a coated plant essential oil composition and coated tannic acid, wherein the coated plant essential oil composition is prepared from an adsorbed plant essential oil composition and a coating carrier; the plant essential oil composition after adsorption is prepared from the plant essential oil composition and an adsorbent; the plant essential oil composition is prepared from carvacrol, thymol and cinnamaldehyde; the coated tannic acid is made from tannic acid and a coating carrier. The invention also discloses a preparation method and application of the livestock and poultry feed additive. The feed additive for livestock and poultry has obvious improvement and treatment effects on livestock and poultry animal organisms, can meet the growth requirements of the livestock and poultry animals, promote the growth speed of the livestock and poultry animals, improve the immunity of the livestock and poultry animals, prevent and treat diarrhea, and does not generate drug resistance, drug residue and toxic or side effect after long-term use.)

1. The feed additive for livestock and poultry is characterized by being prepared from a coated plant essential oil composition and coated tannic acid according to a weight ratio of 1 (1-6), wherein the coated plant essential oil composition is prepared from 52-68 parts by weight of the adsorbed plant essential oil composition and 32-48 parts by weight of a coating carrier; the adsorbed plant essential oil composition is prepared from 12-33 parts by weight of plant essential oil composition and 35-40 parts by weight of adsorbent; the plant essential oil composition is prepared from 1-8 parts by weight of carvacrol, 1-5 parts by weight of thymol and 10-20 parts by weight of cinnamaldehyde; the coated tannic acid is prepared from 40-70 parts by weight of tannic acid and 30-60 parts by weight of a coating carrier.

2. The feed additive for livestock and poultry according to claim 1, which is prepared from a coated plant essential oil composition and coated tannic acid at a weight ratio of 1:1, wherein the coated plant essential oil composition is prepared from 52 parts by weight of the adsorbed plant essential oil composition and 48 parts by weight of a coating carrier; the adsorbed plant essential oil composition is prepared from 17 parts by weight of plant essential oil composition and 35 parts by weight of adsorbent; the plant essential oil composition is prepared from 2.5 parts by weight of carvacrol, 2.5 parts by weight of thymol and 12 parts by weight of cinnamaldehyde; the coated tannic acid is made of 40 parts by weight of tannic acid and 60 parts by weight of a coating carrier.

3. The feed additive for livestock and poultry according to claim 1, which is prepared from a coated plant essential oil composition and coated tannic acid at a weight ratio of 1:5, wherein the coated plant essential oil composition is prepared from 56 parts by weight of the adsorbed plant essential oil composition and 44 parts by weight of a coating carrier; the adsorbed plant essential oil composition is prepared from 20 parts by weight of plant essential oil composition and 36 parts by weight of adsorbent; the plant essential oil composition is prepared from 5 parts by weight of carvacrol, 2.5 parts by weight of thymol and 12.5 parts by weight of cinnamaldehyde; the coated tannic acid is made of 55 parts by weight of tannic acid and 45 parts by weight of a coating carrier.

4. The feed additive for livestock and poultry according to claim 1, which is prepared from a coated plant essential oil composition and coated tannic acid at a weight ratio of 1:3, wherein the coated plant essential oil composition is prepared from 58 parts by weight of the adsorbed plant essential oil composition and 42 parts by weight of a coating carrier; the adsorbed plant essential oil composition is prepared from 21 parts by weight of plant essential oil composition and 37 parts by weight of adsorbent; the plant essential oil composition is prepared from 1.5 parts by weight of carvacrol, 1.5 parts by weight of thymol and 18 parts by weight of cinnamaldehyde; the coated tannic acid is made of tannic acid 70 parts by weight and a coating carrier 30 parts by weight.

5. The feed additive for livestock and poultry according to any one of claims 1 to 4, wherein the carvacrol is plant essential oil obtained by distillation and extraction of oregano, and the content of active ingredients is more than 96%; the thymol is plant essential oil crystal separated from oregano, and the content of effective components is more than 96%; the cinnamaldehyde is effective components extracted from cinnamon or cinnamaldehyde synthesized by a chemical method, and the content of the effective components is more than 99%; the content of effective components in the tannic acid is more than 90%.

6. The livestock and poultry feed additive according to any one of claims 1 to 4, wherein the coating carrier is any one of stearic acid, a salt compound of stearic acid and an enteric slow-release coating material; the adsorbent is any one or a mixture of more of white carbon black, cyclodextrin, modified starch and porous starch.

7. The feed additive for livestock and poultry according to claim 6, wherein the stearic acid is C₁₆-C₁₈Any one of fatty acid, glyceryl monostearate, hydrogenated fatty acid and fatty alcohol; the enteric slow-release packaging material is polypropylene resin IV or chitosan.

8. A preparation method of the feed additive for livestock and poultry is characterized by comprising the following steps:

step 1: preparation of coated plant essential oil composition

Respectively weighing 1-8 parts by weight of carvacrol, 1-5 parts by weight of thymol and 10-20 parts by weight of cinnamaldehyde, and melting to obtain a molten plant essential oil composition;

taking 12-33 parts by weight of the melted plant essential oil composition, adding 35-40 parts by weight of an adsorbent for adsorption to obtain an adsorbed plant essential oil composition;

taking the coating carrier, and melting to obtain a molten coating carrier;

taking 52-68 parts by weight of the adsorbed plant essential oil composition, and adding 32-48 parts by weight of the molten coating carrier to obtain a coated plant essential oil composition;

step 2: preparation of coated tannic acid

Crushing tannic acid to obtain crushed tannic acid;

taking the coating carrier, and melting to obtain a molten coating carrier;

taking 40-70 parts by weight of the crushed tannic acid and 30-60 parts by weight of the fused coating carrier, and extruding and granulating to obtain coated tannic acid;

and step 3: preparation of feed additive for livestock and poultry

And (3) uniformly mixing the coated plant essential oil composition obtained in the step (1) and the coated tannic acid obtained in the step (2) according to the weight ratio of 1 (1-6) to obtain the feed additive for livestock and poultry.

9. Use of the feed additive for livestock and poultry according to any one of claims 1 to 7 for the preparation of a feed for livestock and poultry.

10. The use as claimed in claim 9, wherein the additive amount of the livestock and poultry feed additive is 100g to 3000g per 1 ton of the livestock and poultry feed.

Technical Field

The invention relates to a feed additive for livestock and poultry, a preparation method and application thereof, and belongs to the technical field of feed additives for animals.

Background

With the rapid development of national economy and the increasing improvement of the living standard of people, people put forward higher and more updated requirements on the breeding environment and safe and healthy animal products. The feed additive is an indispensable key substance for realizing the complete nutrition of animals, and is widely used in livestock and poultry and aquaculture. The antibiotics are widely applied as feed additives and play a key role in preventing animal diseases, promoting animal growth and the like, but the long-term use of the antibiotics can cause drug resistance and drug residue, and has the problems of strong toxic and side effects, high treatment expense and the like.

In recent years, alternatives to antibiotics have been sought to achieve antibiotic effects. At present, the application of plant essential oil in feed additives also achieves certain effect, but research is mostly focused on the aspects of single kind of plant essential oil or the mixture of a plurality of kinds of plant essential oil. In the prior art, no feed additive prepared by compounding plant essential oil composition and tannic acid is reported.

In view of this, there is a need to provide a new feed additive for livestock and poultry to solve the deficiencies of the prior art.

Disclosure of Invention

The invention aims to provide a feed additive for livestock and poultry. The feed additive for livestock and poultry can be used for preparing feed for livestock and poultry, has obvious improvement and treatment effects on organisms of the livestock and poultry, can meet the growth requirements of the livestock and poultry, promotes the growth speed of the livestock and poultry, can improve the immunity of the livestock and poultry, prevents and treats diarrhea, and does not generate drug resistance, drug residue and toxic and side effects after long-term use.

The technical scheme for solving the technical problems is as follows: a feed additive for livestock and poultry is prepared from a coated plant essential oil composition and coated tannic acid according to a weight ratio of 1 (1-6), wherein the coated plant essential oil composition is prepared from 52-68 parts by weight of the adsorbed plant essential oil composition and 32-48 parts by weight of a coating carrier; the adsorbed plant essential oil composition is prepared from 12-33 parts by weight of plant essential oil composition and 35-40 parts by weight of adsorbent; the plant essential oil composition is prepared from 1-8 parts by weight of carvacrol, 1-5 parts by weight of thymol and 10-20 parts by weight of cinnamaldehyde; the coated tannic acid is prepared from 40-70 parts by weight of tannic acid and 30-60 parts by weight of a coating carrier.

The principle of the feed additive for livestock and poultry of the invention is as follows:

carvacrol is colorless to light yellow thick oily liquid, has thymol smell, and has molecular formula of C₁₀H₁₄O, molecular weight 150.22, CAS number 499-75-2, chemical formula:

natural carvacrol is mainly present in various labiatae plants, such as thyme, origanum, etc. Carvacrol has effects of clearing heat, eliminating dampness, dispelling summerheat, relieving exterior syndrome, killing bacteria, and inhibiting bacteria.

Thymol, also known as thymol, is white to pale yellow crystalline powder having a molecular formula C at room temperature₁₀H₁₄O, molecular weight 150.22, CAS number 89-83-8, chemical formula:

thymol has antibacterial, antiseptic, antioxidant, antithrombotic and acaricidal effects.

Cinnamic aldehyde, also known as phenylpropenoic aldehyde, cinnamic aldehyde, is a yellow viscous liquid with molecular formula C₉H₈O, molecular weight 132.16, CAS number 104-55-2, chemical formula:

the cinnamaldehyde has strong inhibiting and killing effects on various fungi and mould fungi, but is insoluble in water, volatile, easy to oxidize and preserve, and has strong pungent and spicy taste, so that the using effect of the cinnamaldehyde is influenced.

According to the plant essential oil composition, carvacrol, thymol and cinnamaldehyde are used as raw materials, and the plant essential oil composition has a good antibacterial effect on staphylococcus aureus, salmonella enteritidis, pathogenic escherichia coli and clostridium perfringens according to a limited weight ratio. However, carvacrol, thymol and cinnamaldehyde all have strong pungent smells, which also cause poor palatability and influence the feed intake of livestock and poultry. Therefore, in the invention, the carvacrol, the thymol and the cinnamaldehyde are subjected to slow-release coating treatment by the coating carrier, so that the carvacrol, the thymol and the cinnamaldehyde exist in a slow-release agent form, the release of the carvacrol, the thymol and the cinnamaldehyde can be effectively delayed, the action time of the carvacrol, the thymol and the cinnamaldehyde in the bodies of the livestock and poultry animals is prolonged, and the carvacrol, the thymol and the cinnamaldehyde can continuously play a role in the bodies of the livestock and poultry animals.

Tannic acid, also known as tannic acid, gallotannic acid, larch baking gum, digallic acid, charinic acid, etc., is a complex class of high molecular polyphenol compounds. Molecular formula C₇₆H₅₂O₄₆Molecular weight is 1701.20, CAS number is 1401-55-4, chemical structural formula is:

the tannic acid has the effects of astringency, antibiosis, antivirus, antioxidation, antitumor and the like, can effectively control diarrhea, and has strong inhibiting effect on harmful microorganisms in intestinal tracts; can precipitate protein, form insoluble complex with heavy metal, alkaloid and glucoside, etc., and has toxic materials clearing away effect; can decompose antinutritional factors, promote absorption of protein and mineral nutrients by animals, and improve growth performance. In the invention, the tannic acid is added, so that the micro-ecological environment of intestinal tracts of livestock and poultry can be improved, the growth of the livestock and poultry is promoted, and the utilization rate of nutrient substances is increased. However, tannic acid has strong astringency, which causes poor palatability and affects feed intake of livestock and poultry. In the invention, the tannic acid is subjected to slow-release coating treatment by the coating carrier and is granulated, so that the tannic acid exists in the form of a slow-release agent, the release of the tannic acid can be effectively delayed, the action time of the tannic acid in livestock and poultry bodies is prolonged, and the tannic acid can continuously play a role in the livestock and poultry bodies.

In the prior art, no report is provided for a feed additive compounded by a vegetable essential oil composition and tannic acid, wherein carvacrol, thymol and cinnamaldehyde are taken as raw materials. The feed additive takes the coated plant essential oil composition and the coated tannic acid as raw materials, and has the effects of enhancing the antibacterial effect and improving the immunity of livestock and poultry through the limited weight proportion.

The raw materials involved in the invention are all commercially available.

The feed additive for livestock and poultry has the beneficial effects that:

1. the feed additive for livestock and poultry can be used for preparing feed for livestock and poultry, has obvious improvement and treatment effects on organisms of livestock and poultry animals, can meet the growth requirements of the livestock and poultry animals, promote the growth speed of the livestock and poultry animals, improve the immunity of the livestock and poultry animals, prevent and treat diarrhea, and does not generate drug resistance, drug residue and toxic and side effects after long-term use.

2. The feed additive for livestock and poultry has the advantages of easily available raw materials and low cost, and can play an important role in animal husbandry production as a substitute product of the antibacterial agent.

On the basis of the technical scheme, the invention can be further improved as follows.

Further, the coating is prepared from a coated plant essential oil composition and coated tannic acid according to a weight ratio of 1:1, wherein the coated plant essential oil composition is prepared from 52 parts by weight of the adsorbed plant essential oil composition and 48 parts by weight of a coating carrier; the adsorbed plant essential oil composition is prepared from 17 parts by weight of plant essential oil composition and 35 parts by weight of adsorbent; the plant essential oil composition is prepared from 2.5 parts by weight of carvacrol, 2.5 parts by weight of thymol and 12 parts by weight of cinnamaldehyde; the coated tannic acid is made of 40 parts by weight of tannic acid and 60 parts by weight of a coating carrier.

The adoption of the further beneficial effects is as follows: the above parameters are the best parameters, and the obtained feed additive for livestock and poultry has the best effect.

Further, the coating is prepared from a coated plant essential oil composition and coated tannic acid according to a weight ratio of 1:5, wherein the coated plant essential oil composition is prepared from 56 parts by weight of the adsorbed plant essential oil composition and 44 parts by weight of a coating carrier; the adsorbed plant essential oil composition is prepared from 20 parts by weight of plant essential oil composition and 36 parts by weight of adsorbent; the plant essential oil composition is prepared from 5 parts by weight of carvacrol, 2.5 parts by weight of thymol and 12.5 parts by weight of cinnamaldehyde; the coated tannic acid is made of 55 parts by weight of tannic acid and 45 parts by weight of a coating carrier.

The adoption of the further beneficial effects is as follows: the above parameters are the best parameters, and the obtained feed additive for livestock and poultry has the best effect.

Further, the coating is prepared from a coated plant essential oil composition and coated tannic acid according to a weight ratio of 1:3, wherein the coated plant essential oil composition is prepared from 58 parts by weight of the adsorbed plant essential oil composition and 42 parts by weight of a coating carrier; the adsorbed plant essential oil composition is prepared from 21 parts by weight of plant essential oil composition and 37 parts by weight of adsorbent; the plant essential oil composition is prepared from 1.5 parts by weight of carvacrol, 1.5 parts by weight of thymol and 18 parts by weight of cinnamaldehyde; the coated tannic acid is made of 70 parts by weight of tannic acid and 30 parts by weight of a coating carrier.

The adoption of the further beneficial effects is as follows: the above parameters are the best parameters, and the obtained feed additive for livestock and poultry has the best effect.

Further, the carvacrol is plant essential oil obtained by distilling and extracting origanum vulgare, and the content of active ingredients is more than 96%.

The adoption of the further beneficial effects is as follows: the carvacrol has high purity, and can ensure the stability of the feed additive product for livestock and poultry.

In the invention, the carvacrol can be purchased in the market, for example, the carvacrol can be purchased from Nanjing Jiulong trade Limited company, the specification is 200 kg/barrel, and the content of the effective component of the carvacrol is 99%.

Further, the thymol is plant essential oil crystal separated from oregano, and the content of effective component is more than 96%.

The adoption of the further beneficial effects is as follows: the thymol has high purity, and can ensure the stability of the feed additive product for livestock and poultry.

In the present invention, thymol is commercially available, such as available from the limited trade company of Jiulonghui Nanjing, with a specification of 25 kg/barrel and a thymol active ingredient content of 99%.

Further, the cinnamaldehyde is effective components extracted from cinnamon or cinnamaldehyde synthesized by a chemical method, and the content of the effective components is more than 99%.

The adoption of the further beneficial effects is as follows: the cinnamaldehyde has high purity, and can ensure the stability of the feed additive product for livestock and poultry.

In the invention, the cinnamaldehyde can be purchased in the market, for example, the cinnamaldehyde can be purchased from limited company of the Jiulonghui trade of Nanjing, the specification is 220 kg/barrel, and the content of active ingredients of the cinnamaldehyde is 99 percent.

Further, the content of effective components in the tannic acid is more than 90%.

The adoption of the further beneficial effects is as follows: the performance of the feed additive for livestock and poultry obtained by adopting the tannic acid with the parameters is better.

In the present invention, tannic acid is commercially available, for example, from Chicheng Biotech Co., Ltd, in a package size of 20 kg/bag, and has a content of tannic acid as an effective component of 93%.

Further, the coating carrier is any one of stearic acid, a salt compound of stearic acid and an enteric slow-release coating material.

The beneficial effect of adopting the further scheme is that: the coating carrier adopts stearic acid or enteric-soluble slow-release coating materials, and the hydrophilic and oleophilic characteristics of the coating materials are utilized to achieve the effect of emulsification and solubilization, so that carvacrol, thymol and cinnamic aldehyde can effectively play a role in intestinal tracts, the volatility of plant essential oil can be reduced, the palatability is improved, and the mixing uniformity and stability of the feed additive product for livestock and poultry are ensured.

Further, the stearic acid is C₁₆-C₁₈Any one of fatty acid, glyceryl monostearate, hydrogenated fatty acid and fatty alcohol.

The further beneficial effects of the adoption are as follows: c above₁₆-C₁₈The fatty acids may be purchased commercially, for example from the Langshan International trade company, Inc. at a 25 kg/bag format.

The hydrogenated fatty acid may be purchased commercially, for example from Tianjin Nuoist trade company Limited, at a specification of 25 kg/bag.

Furthermore, the enteric slow-release packaging material is polypropylene resin IV or chitosan.

The further beneficial effects of the adoption are as follows: the polypropylene resin IV can be purchased commercially, for example, from Shanghai Chang as pharmaceutical adjuvant technology limited company with a specification of 20 kg/barrel.

The chitosan can be purchased commercially, such as from Hippon Highgloss Biotechnology Inc., with a specification of 25 kg/barrel.

Further, the adsorbent is one or a mixture of more of white carbon black, cyclodextrin, modified starch and porous starch.

The beneficial effect of adopting the further scheme is that: the adsorbent can uniformly adsorb the plant essential oil composition, and effectively avoid volatilization of carvacrol, thymol and cinnamaldehyde.

The second object of the present invention is to provide a method for preparing the above-mentioned livestock and poultry feed additive. The preparation method is simple, easy to operate, wide in market prospect and suitable for large-scale popularization and application.

The technical scheme for solving the technical problems is as follows: a preparation method of a feed additive for livestock and poultry comprises the following steps:

step 1: preparation of coated plant essential oil composition

Respectively weighing 1-8 parts by weight of carvacrol, 1-5 parts by weight of thymol and 10-20 parts by weight of cinnamaldehyde, and melting to obtain a molten plant essential oil composition;

taking 12-33 parts by weight of the melted plant essential oil composition, adding 35-40 parts by weight of an adsorbent for adsorption to obtain an adsorbed plant essential oil composition;

taking the coating carrier, and melting to obtain a molten coating carrier;

taking 52-68 parts by weight of the adsorbed plant essential oil composition, and adding 32-48 parts by weight of the molten coating carrier to obtain a coated plant essential oil composition;

step 2: preparation of coated tannic acid

Crushing tannic acid to obtain crushed tannic acid;

taking the coating carrier, and melting to obtain a molten coating carrier;

taking 40-70 parts by weight of the crushed tannic acid and 30-60 parts by weight of the fused coating carrier, and extruding and granulating to obtain coated tannic acid;

and step 3: preparation of feed additive for livestock and poultry

And (3) uniformly mixing the coated plant essential oil composition obtained in the step (1) and the coated tannic acid obtained in the step (2) according to the weight ratio of 1 (1-6) to obtain the feed additive for livestock and poultry.

The preparation method of the feed additive for livestock and poultry has the beneficial effects that:

the preparation method is simple, easy to operate, low in cost, wide in market prospect and suitable for large-scale popularization and application.

The third purpose of the invention is to provide the application of the livestock and poultry feed additive. The livestock and poultry feed additive can be used for preparing livestock and poultry feed, has good mixing uniformity after being added into the feed, does not influence the feed quality, can be used as a feed flavoring agent, can improve the feed intake of livestock and poultry, increase daily gain, reduce the feed conversion ratio, has obvious economic benefit, regulates the intestinal flora of animals, and improves the immunity of organisms.

The technical scheme for solving the technical problems is as follows: the application of the feed additive for livestock and poultry in preparing the feed for livestock and poultry.

The application of the feed additive for livestock and poultry has the beneficial effects that:

the feed additive for livestock and poultry can be used for preparing feed for livestock and poultry, has good mixing uniformity after being added into the feed, does not influence the quality of the feed, can be used as a feed flavoring agent, can improve the feed intake of livestock and poultry, increase the daily gain, reduce the feed conversion ratio, has obvious economic benefit, regulates the intestinal flora of animals, and improves the immunity of organisms

On the basis of the technical scheme, the invention can be further improved as follows.

Furthermore, the addition amount of the feed additive for livestock and poultry is 100g-3000g in every 1 ton of the feed for livestock and poultry.

The adoption of the further beneficial effects is as follows: by adopting the parameters, the growth speed of the livestock and poultry can be obviously improved, and the immunity of the livestock and poultry can also be improved.

Detailed Description

The principles and features of this invention are described below in conjunction with specific embodiments, which are set forth merely to illustrate the invention and are not intended to limit the scope of the invention.

Example 1

The feed additive for livestock and poultry is prepared from a coated plant essential oil composition and coated tannic acid according to a weight ratio of 1:1, wherein the coated plant essential oil composition is prepared from 520g of adsorbed plant essential oil composition and 480g of glyceryl monostearate; the adsorbed plant essential oil composition is prepared from 170g of plant essential oil composition and 350g of white carbon black; the plant essential oil composition is prepared from 25g of carvacrol, 25g of thymol and 120g of cinnamaldehyde; the coated tannic acid was made of tannic acid 400g and hydrogenated fatty acid 600 g.

The preparation method of the feed additive for livestock and poultry comprises the following steps:

step 1: preparation of coated plant essential oil composition

Respectively weighing 25g of parsley, 25g of thymol and 120g of cinnamaldehyde, heating to 90 ℃ and melting to obtain a molten plant essential oil composition.

And (3) adding 350g of white carbon black into 170g of the melted plant essential oil composition for adsorption to obtain the adsorbed plant essential oil composition.

Heating glyceryl monostearate to 90 deg.C to melt to obtain molten glyceryl monostearate.

And (3) taking 520g of the adsorbed plant essential oil composition, and adding 480g of the melted glyceryl monostearate to obtain a coated plant essential oil composition.

Step 2: preparation of coated tannic acid

Pulverizing tannic acid to 80 mesh to obtain pulverized tannic acid.

Heating hydrogenated fatty acid to 120 deg.c to melt to obtain molten hydrogenated fatty acid.

Taking 400g of the crushed tannic acid and 600g of the molten hydrogenated fatty acid, and extruding and granulating to obtain the coated tannic acid.

And step 3: preparation of feed additive for livestock and poultry

And (3) uniformly mixing the coated plant essential oil composition obtained in the step (1) and the coated tannic acid obtained in the step (2) according to the weight ratio of 1:1 to obtain the feed additive for livestock and poultry.

The application of the feed additive for livestock and poultry in preparing the feed for livestock and poultry.

The additive amount of the livestock and poultry feed additive is 2000g in every 1 ton of the livestock and poultry feed.

Example 2

The feed additive for livestock and poultry of the embodiment is prepared from a coated plant essential oil composition and coated tannic acid according to a weight ratio of 1:5, wherein the coated plant essential oil composition is prepared from 560g of adsorbed plant essential oil composition and 440g of hydrogenated fatty acid; the absorbed plant essential oil composition is prepared from 200g of plant essential oil composition and 360g of white carbon black; the plant essential oil composition is prepared from 50g of carvacrol, 25g of thymol and 125g of cinnamaldehyde; the coated tannic acid was made of 550g of tannic acid and 450g of hydrogenated fatty acid.

The preparation method of the feed additive for livestock and poultry comprises the following steps:

step 1: preparation of coated plant essential oil composition

Weighing 50g of parsley, 25g of thymol and 125g of cinnamaldehyde respectively, and heating to 90 ℃ to melt to obtain a molten plant essential oil composition.

And (3) adding 360g of white carbon black into 200g of the melted plant essential oil composition for adsorption to obtain the adsorbed plant essential oil composition.

Heating hydrogenated fatty acid to 90 deg.c to melt to obtain molten hydrogenated fatty acid.

And (3) adding 560g of the adsorbed plant essential oil composition into 440g of the molten hydrogenated fatty acid to obtain a coated plant essential oil composition.

Step 2: preparation of coated tannic acid

Pulverizing tannic acid to 80 mesh to obtain pulverized tannic acid.

Heating hydrogenated fatty acid to 120 deg.c to melt to obtain molten hydrogenated fatty acid.

And taking 550g of the crushed tannic acid and 450g of the molten hydrogenated fatty acid, and extruding and granulating to obtain the coated tannic acid.

And step 3: preparation of feed additive for livestock and poultry

And (3) uniformly mixing the coated plant essential oil composition obtained in the step (1) and the coated tannic acid obtained in the step (2) according to the weight ratio of 1:5 to obtain the feed additive for livestock and poultry.

The application of the feed additive for livestock and poultry in preparing the feed for livestock and poultry.

The additive amount of the livestock and poultry feed additive is 600g in every 1 ton of the livestock and poultry feed.

Example 3

The feed additive for livestock and poultry is prepared from a coated plant essential oil composition and coated tannic acid according to a weight ratio of 1:3, wherein the coated plant essential oil composition is prepared from 580g of adsorbed plant essential oil composition and 420g of glyceryl monostearate; the adsorbed plant essential oil composition is prepared from 210g of plant essential oil composition and 370g of white carbon black; the plant essential oil composition is prepared from 15g of carvacrol, 15g of thymol and 180g of cinnamaldehyde; the coated tannic acid was made of tannic acid 700g and hydrogenated fatty acid 300 g.

The preparation method of the feed additive for livestock and poultry comprises the following steps:

step 1: preparation of coated plant essential oil composition

Weighing 15g of parsley, 15g of thymol and 180g of cinnamaldehyde respectively, and heating to 90 ℃ to melt to obtain a molten plant essential oil composition.

And (3) adding 360g of white carbon black into 200g of the melted plant essential oil composition for adsorption to obtain the adsorbed plant essential oil composition.

Heating glyceryl monostearate to 90 deg.C to melt to obtain molten glyceryl monostearate.

And (3) adding 420g of the melted glyceryl monostearate into 580g of the adsorbed plant essential oil composition to obtain a coated plant essential oil composition.

Step 2: preparation of coated tannic acid

Pulverizing tannic acid to 80 mesh to obtain pulverized tannic acid.

Heating hydrogenated fatty acid to 120 deg.c to melt to obtain molten hydrogenated fatty acid.

Taking 700g of the crushed tannic acid and 300g of the molten hydrogenated fatty acid, and extruding and granulating to obtain the coated tannic acid.

And step 3: preparation of feed additive for livestock and poultry

And (3) uniformly mixing the coated plant essential oil composition obtained in the step (1) and the coated tannic acid obtained in the step (2) according to the weight ratio of 1:3 to obtain the feed additive for livestock and poultry.

The application of the feed additive for livestock and poultry in preparing the feed for livestock and poultry.

The additive amount of the livestock and poultry feed additive is 1000g in every 1 ton of the livestock and poultry feed.

Experimental example 1

1 Experimental materials and methods

1.1 test strains

Staphylococcus aureus, Salmonella pullorum, pathogenic Escherichia coli, and Clostridium perfringens.

1.2 materials

Sample nos. 1 to 12: plant essential oil composition, plant essential oil composition + tannic acid product.

1.3 Medium

Nutrient broth, nutrient agar, Columbia blood plates and liquid thioglycollate culture medium.

Purpose of the experiment

(1) Effects of plant essential oil composition and tannin on staphylococcus aureus, salmonella pullorum, pathogenic escherichia coli and clostridium perfringens are determined.

(2) Plating to determine bacterial liquid concentration of 10⁸cfu/mL。

3 the concrete operation steps are as follows:

(1) weighing 18g of nutrient broth, 1000mL of distilled water and sterilizing at 121 ℃ for 15min for later use. 33g of nutrient agar and 1000mL of distilled water are taken and poured into a plate for standby. Taking 25g of liquid thioglycollate culture medium, distilling 1000mL of water, and sterilizing at 121 ℃ for 15 min.

(2) Inoculating frozen thallus (Staphylococcus aureus, Salmonella pullorum, and pathogenic Escherichia coli) into a triangular flask containing 50mL broth liquid culture medium, and shake culturing in a shaker at 37 deg.C for 18-24 h; inoculating frozen thallus (Clostridium perfringens) into a triangular flask containing 50mL of liquid thioglycollate medium, and culturing in an anaerobic incubator at 37 ℃ for 18-24 h.

(3) Mixing the bacterial suspension and the sterile liquid culture medium according to the volume ratio of 1: 9, mixing uniformly to prepare bacterial suspension with proper concentration, diluting the mother liquor according to a certain proportion, taking 15 sterilized test tubes, numbering in sequence, adding 1.6mL of liquid culture medium into the first tube, and adding 1.0mL into the other tubes. Then, the test solution was added to each test tube. 0.4mL of the liquid medicine was added to the 1 st tube, and after mixing, 1.0mL of the liquid medicine was aspirated and added to the 2 nd tube. The tube is diluted to 14 tubes and 1.0mL is discarded, and the 15 th tube is a growth control tube. Adding 0.4mL of ethanol as a blank control into the 15 th tube, fully mixing, adding 1.0mL of bacterial suspension, plugging the tube opening of the test tube with a sterilized rubber plug, and placing the test tube in an incubator at 37 ℃ for culturing for 18-24h while performing 4 parallels.

TABLE 1 minimum inhibitory concentration (sample No. 1-12)

4 analysis of results

4.1 the test shows that the plant essential oil composition, the plant essential oil composition and the tannic acid have the antibacterial effects on escherichia coli, staphylococcus aureus, clostridium perfringens and salmonella pullorum, the Minimum Inhibitory Concentration (MIC) of each combination is determined, the optimal matching combination of the plant essential oil composition and the tannic acid is determined, and basic theoretical data support is provided for the plant essential oil composition and the tannic acid in livestock and poultry breeding and field application.

4.2 the minimum inhibitory concentrations of the solvent (sample No. 2 ethanol) on Escherichia coli, Staphylococcus aureus, Clostridium perfringens and Salmonella pullorum were 1250. mu.g/mL, 1250. mu.g/mL and 1250. mu.g/mL, respectively.

4.3 minimum inhibitory concentrations of the plant essential oil composition (sample No. 1) against Escherichia coli, Staphylococcus aureus, Clostridium perfringens and Salmonella pullorum were 125. mu.g/mL, 125. mu.g/mL and 125. mu.g/mL, respectively.

4.4 the bacteriostatic results of the plant essential oil composition (50g) and different combinations of tannic acid and the plant essential oil composition (100g) and different combinations of tannic acid show that the bacteriostatic effect of the plant essential oil composition on tannic acid is not obvious due to the low concentration of the plant essential oil, but the bacteriostatic effects of the four bacteria are enhanced to different degrees due to the concentration of tannic acid varying from 100g to 500 g.

4.5 As can be seen from the table, the bacteriostatic results of the plant essential oil composition (100g) and the tannin show that the minimum bacteriostatic concentration is better than that of the plant essential oil composition (the minimum bacteriostatic concentration is 125 mug/mL) aiming at different bacteria, which shows that the bacteriostatic effect of the combined additive on different bacteria is obviously enhanced.

Experimental example 2: influence of plant essential oil and tannic acid on growth performance of broiler chicken

1 Experimental materials and groups

Plant essential oil and tannin for experiments are provided by the company, and products are treated by the method of the embodiment. The experiment was carried out in a Hefeng farm. 384 feathers of 8-day-old cocks in the same batch, healthy and similar weight are selected for the experiment, and are randomly divided into 4 treatments according to the weight of each chick, each treatment is 12 repetitions (each cage position is one repetition), each repetition is 8 chickens, three layers of cage culture are adopted, and the experiment period is 28 days. Since the death and culling rate of the chicks is relatively high 7 days before the chicks, the experiment is not avoided to be influenced, so that the experiment is recommended to be divided into 8-28 days old and grouped from 8 days, and the specific grouping and processing are shown in the following table 1.

TABLE 1 packet case

Item	Treatment (8-28 days)
		Process one	Basal diet
Treatment two	Basal diet and 100g/t plant essential oil composition
		Treatment three	Basal diet and 500g/t tannic acid
Treatment four	Basic feed, 100g/t plant essential oil composition and 500g/t tannic acid

2 measurement of production Performance index

The materials are weighed at regular time every day, and the feed intake of each cage position every day is recorded. Each of the dead chickens, which appeared repeatedly, was weighed daily and recorded, and after fasting at 28 days of age, was weighed on an empty stomach in the morning, and the Average Daily Gain (ADG), Average Daily Feed Intake (ADFI), and feed-to-weight ratio (F/G) were calculated for the chickens at each stage based on the initial weight, final weight, and daily feed intake.

Average Daily Gain (ADG) ═ weight at end of trial phase-initial weight at trial phase/total days at trial phase/number of chickens.

Average Daily Food Intake (ADFI) total food intake during the trial period/total days during the trial period/number of chickens.

Feed weight ratio (F/G) average daily feed intake/average daily gain.

3 analysis of results

TABLE 2 influence of plant essential oils and tannins on broiler productivity

From table 2, the average daily feed intake of the four treatment groups is respectively increased by 11.64%, 6.32% and 6.96% compared with the former three groups, the average daily gain is respectively increased by 12.03%, 6.68% and 7.32%, and the material weight ratio is respectively reduced by 0.64%, which shows that after the plant essential oil composition is added by 100g/t and the tannic acid is added by 500g/t, the average daily feed intake and the average daily gain are obviously increased compared with the groups which do not add, add the plant essential oil composition alone and add the tannic acid alone, so that the production performance of the broiler can be obviously improved after the two groups are combined, and more economic benefits are created.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.