阅读说明：本技术 一种用于动物脑损伤超声治疗的固定装置及其方法 (Fixing device and method for ultrasonic treatment of animal brain injury ) 是由 彭勇 黄娅萌 赵洋 刘晓月 刘兰祥 赵政 张慧 韩领军 于 2020-12-23 设计创作，主要内容包括：本发明涉及一种用于动物脑损伤超声治疗的固定装置及其方法,所述装置包括放置器、推进器和底座；所述放置器包括外侧筒、平面锥形筒、柱形环、颅骨固定钉、咬合钉和超声孔；所述推进器包括调节螺钉、夹持板和活塞；所述外侧筒、柱形环与平面锥形筒相接；所述平面锥形筒上安装颅骨固定钉,所述平面锥形筒与柱形环衔接处安装咬合钉；所述超声孔位于平面锥形筒上部中心处；所述夹持板分别对应设置在外侧筒和活塞后端的两侧；所述调节螺钉设置在两侧夹持板之间；所述活塞直径与外侧筒的内壁相切。本发明主要解决现有固定装置不能用于脑损伤动物超声治疗以及在动物无麻醉平静状态下进行实验问题,可有效提高实验成功率,具有实际应用价值。(The invention relates to a fixing device for animal brain injury ultrasonic therapy and a method thereof, wherein the device comprises a placer, a propeller and a base; the placer comprises an outer cylinder, a planar conical cylinder, a cylindrical ring, skull fixing nails, occlusion nails and ultrasonic holes; the thruster comprises an adjusting screw, a clamping plate and a piston; the outer cylinder and the cylindrical ring are connected with the planar conical cylinder; a skull fixing nail is arranged on the plane conical cylinder, and an occlusion nail is arranged at the joint of the plane conical cylinder and the cylindrical ring; the ultrasonic hole is positioned in the center of the upper part of the planar conical cylinder; the clamping plates are respectively and correspondingly arranged on two sides of the rear ends of the outer cylinder and the piston; the adjusting screw is arranged between the clamping plates on the two sides; the diameter of the piston is tangent to the inner wall of the outer cylinder. The invention mainly solves the problems that the existing fixing device can not be used for ultrasonic treatment of brain injury animals and experiments are carried out under the condition that the animals are not anesthetized and calm, can effectively improve the success rate of the experiments, and has practical application value.)

1. A fixing device for ultrasonic treatment of animal brain injury is characterized in that: the device comprises a base, a placer and a propeller; the placer comprises an outer cylinder, a planar conical cylinder, a cylindrical ring, skull fixing nails, occlusion nails and ultrasonic holes; the outer side cylinder is arranged on the upper surface of the base, the plane conical cylinder is correspondingly arranged at the front end of the outer side cylinder, the cylindrical ring is correspondingly arranged at the front end of the plane conical cylinder, the skull fixing nails are respectively arranged in the middle of the left side and the right side of the plane conical cylinder and on the upper part of the joint of the cylindrical ring and the plane conical cylinder, the occlusion nail is arranged in the middle of the joint of the cylindrical ring and the plane conical cylinder, and the ultrasonic hole is arranged in the center of the upper part of the plane conical cylinder;

the thruster comprises a piston, a clamping plate and an adjusting screw; the piston is arranged inside the outer side cylinder, the clamping plates are correspondingly arranged on the left side and the right side of the middle part of the rear end of the outer side cylinder and the left side and the right side of the middle part of the rear end of the piston respectively, and the adjusting screws are connected between the clamping plates on the same sides of the outer side cylinder and the piston respectively.

2. A fixture for ultrasonic treatment of brain injury in an animal according to claim 1, wherein: the inner ends of the skull fixing nails are provided with rectangular skull clamping sheets, the skull clamping sheets at the inner ends of the skull fixing nails at the middle parts of the left side and the right side of the plane conical cylinder are parallel to the inner walls of the two sides of the plane conical cylinder respectively, and the skull clamping sheets at the inner ends of the skull fixing nails at the upper part of the joint of the cylindrical rings and the plane conical cylinder are parallel to the base.

3. A fixation device for ultrasonic treatment of brain injuries of animals according to claim 1 or 2, characterized in that: the diameter of the piston is tangent to the inner wall of the outer cylinder.

4. A fixation method for ultrasonic treatment of brain injury in an animal, the method comprising the steps of:

step S1: preparing a moderate traumatic brain injury model of a rat, and carrying out ultrasonic treatment after 24 hours;

step S2: placing the base on a plane, rotating an adjusting screw in the propeller outwards, separating the adjusting screw from the clamping plate, and placing the experimental rat into an outer cylinder;

step S3: adjusting screws are arranged on two sides of the clamping plate, the adjusting screws are rotated inwards, the piston is pushed inwards, the head of the rat sequentially passes through the outer cylinder and the plane conical cylinder inwards, and finally the mouth of the rat reaches the cylindrical ring, so that the mouth of the rat bites the biting nail;

step S4: rotating skull fixing nails on two sides of a planar conical cylinder in the placer towards the inside of the cylinder until two sides of the head of a rat are clamped, and then rotating the skull fixing nails on the upper part of the planar conical cylinder towards the inside of the cylinder until the mouth of the rat is clamped, so that the brain injury part of the experimental rat is aligned with the ultrasonic hole;

step S5: placing an ultrasonic therapeutic instrument probe to a brain injury part through an ultrasonic hole, and carrying out ultrasonic therapy on the rat;

step S6: after ultrasonic treatment, rotating the skull fixing nails at the upper part of the planar conical cylinder in the placer to the outside of the cylinder, then rotating the skull fixing nails at the two sides of the planar conical cylinder to the outside of the cylinder, enabling the skull clamping sheet at the inner end of the skull fixing nail to be attached to the cylinder wall, rotating the adjusting screw outwards, enabling the piston to be drawn out outwards, separating the mouth of the rat from the occlusion nail, and normally feeding;

step S7: two weeks of ultrasound treatment was performed on brain injured rats and experimental data were recorded.

Technical Field

The invention relates to the technical field of biomedical experiments, in particular to a fixing device and a method for ultrasonic treatment of animal brain injury.

Background

Brain damage is a disease that seriously endangers human health, and has high morbidity, disability rate and mortality. In an animal model with brain injury, a rat model is generally taken as an example, and the existing literature shows that ultrasound can effectively treat rats with brain injury, when low-intensity pulses act on brain tissues of rats in ultrasonic treatment, mechanical action, thermal action and cavitation are generated, so that local tissue blood flow of rats is accelerated, blood circulation is improved, peristalsis of blood vessel walls is increased, cell membrane permeability is enhanced, ions are redistributed, metabolism is vigorous, hydrogen ion concentration in tissues is reduced, pH value is increased, enzyme activity is enhanced, tissue regeneration and repair capacity is enhanced, muscles are relaxed, muscle tension is reduced, and pain is relieved or relieved. Meanwhile, recent research results show that the low-intensity transcranial ultrasonic brain stimulation technology with high spatial resolution, high penetration depth and no wound can arouse patients with severe disturbance of consciousness.

Wenhaozhu et al disclose a rat ultrasonic probe fixing device (CN204563349U), mainly be to the probe fixing device of brain injury rat ultrasonic therapy for with ultrasonic probe laminating is fixed on the rat, but because the probe can move along with the removal of rat, can not guarantee to be in static state when the transcranial ultrasonic therapy. The Sping red discloses a basic experiment rat fixer (CN109223238A), which comprises an outer cylinder, a rear baffle and an inner mesh enclosure, wherein the rear side wall of the outer cylinder is provided with the rear baffle, the inner mesh enclosure is arranged in the front end of the outer cylinder, and a plurality of rat four-limb extension windows are arranged on two sides of the lower part of the outer cylinder. The patent refers to the field of 'medical instruments or methods for the treatment of cancer, or cancer'. Therefore, the existing solutions have certain disadvantages and have further improved and perfect space.

Disclosure of Invention

In view of the above problems, an object of the present invention is to provide a fixing device for ultrasonic therapy of brain injury of animals and a method thereof, which solve the problem of lack of a fixing device for ultrasonic therapy of rats with brain injury, solve the problem of side effects caused by using anesthetic for rats due to long-term ultrasonic therapy of rats with brain injury, reduce the experimental cost of transcranial ultrasonic therapy of brain injury, and improve the success rate of experiments.

The technical scheme adopted by the invention is as follows:

the invention provides a fixing device for ultrasonic treatment of animal brain injury, which comprises a base, a placer and a propeller; the placer comprises an outer cylinder, a planar conical cylinder, a cylindrical ring, skull fixing nails, occlusion nails and ultrasonic holes; the outer side cylinder is arranged on the upper surface of the base, the plane conical cylinder is correspondingly arranged at the front end of the outer side cylinder, the cylindrical ring is correspondingly arranged at the front end of the plane conical cylinder, the skull fixing nails are respectively arranged in the middle of the left side and the right side of the plane conical cylinder and on the upper part of the joint of the cylindrical ring and the plane conical cylinder, the occlusion nail is arranged in the middle of the joint of the cylindrical ring and the plane conical cylinder, and the ultrasonic hole is arranged in the center of the upper part of the plane conical cylinder;

the thruster comprises a piston, a clamping plate and an adjusting screw; the piston is arranged inside the outer side cylinder, the clamping plates are correspondingly arranged on the left side and the right side of the middle part of the rear end of the outer side cylinder and the left side and the right side of the middle part of the rear end of the piston respectively, and the adjusting screws are connected between the clamping plates on the same sides of the outer side cylinder and the piston respectively.

Furthermore, rectangular skull clamping sheets are arranged at the inner ends of the skull fixing nails, the skull clamping sheets at the inner ends of the skull fixing nails at the middle parts of the left side and the right side of the plane conical cylinder are parallel to the inner walls of the two sides of the plane conical cylinder respectively, and the skull clamping sheets at the inner ends of the skull fixing nails at the upper part of the joint of the columnar rings and the plane conical cylinder are parallel to the base.

Further, the diameter of the piston is tangent to the inner wall of the outer cylinder.

A fixation method for ultrasonic treatment of brain injury in an animal, the method comprising the steps of:

step S1: preparing a moderate traumatic brain injury model of a rat, and carrying out ultrasonic treatment after 24 hours;

step S2: placing the base on a plane, rotating an adjusting screw in the propeller outwards, separating the adjusting screw from the clamping plate, and placing the experimental rat into an outer cylinder;

step S3: adjusting screws are arranged on two sides of the clamping plate, the adjusting screws are rotated inwards, the piston is pushed inwards, the head of the rat sequentially passes through the outer cylinder and the plane conical cylinder inwards, and finally the mouth of the rat reaches the cylindrical ring, so that the mouth of the rat bites the biting nail;

step S4: rotating skull fixing nails on two sides of a planar conical cylinder in the placer towards the inside of the cylinder until two sides of the head of a rat are clamped, and then rotating the skull fixing nails on the upper part of the planar conical cylinder towards the inside of the cylinder until the mouth of the rat is clamped, so that the brain injury part of the experimental rat is aligned with the ultrasonic hole;

step S5: placing an ultrasonic therapeutic instrument probe to a brain injury part through an ultrasonic hole, and carrying out ultrasonic therapy on the rat;

step S6: after ultrasonic treatment, rotating the skull fixing nails at the upper part of the planar conical cylinder in the placer to the outside of the cylinder, then rotating the skull fixing nails at the two sides of the planar conical cylinder to the outside of the cylinder, enabling the skull clamping sheet at the inner end of the skull fixing nail to be attached to the cylinder wall, rotating the adjusting screw outwards, enabling the piston to be drawn out outwards, separating the mouth of the rat from the occlusion nail, and normally feeding;

step S7: two weeks of ultrasound treatment was performed on brain injured rats and experimental data were recorded.

Compared with the prior art, the invention has the following beneficial effects:

the skull cavity is fixedly matched with the occlusion nail and the propeller to enable the animal to be balanced and fixed in all directions, the upper part, the lower part, the left part, the right part, the front part and the back part, so that the experimental operation is convenient, the safety and the effectiveness are improved, the ultrasonic therapeutic instrument probe is placed in the ultrasonic hole to facilitate the ultrasonic therapy of the animal, the experimental stability can be improved by the base of the device, the skull cavity is simple and convenient to operate, the cost is low, the skull cavity is safe and reliable, the success rate of the experiment can be effectively improved.

Drawings

FIG. 1 is a schematic view of the overall structure of one embodiment of a fixing device for ultrasonic treatment of brain injury of an animal according to the present invention;

FIG. 2 is a rear side schematic view of the structure of FIG. 1;

FIG. 3 is a schematic front view of the structure of FIG. 1;

FIG. 4 is a schematic top view of the structure of FIG. 1;

FIG. 5 is a schematic cross-sectional view of FIG. 4;

fig. 6 is a side sectional view of the structure of fig. 1.

Wherein, the reference numbers: 1-a base; 2-an outer cylinder; 3-a planar conical cylinder; 4-cylindrical rings; 5-skull fixation nails; 6-occlusion nail; 7-ultrasonic holes; 8-a piston; 9-a clamping plate; 10-adjusting screws; 11-skull clamping piece.

Detailed Description

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

It should be noted that in the description of the present invention, the terms "upper", "lower", "top", "bottom", "one side", "the other side", "left", "right", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, but do not mean that a device or an element must have a specific orientation, be configured and operated in a specific orientation.

Referring to fig. 1 to 6, a specific structure of an embodiment of a fixing device for ultrasonic therapy of brain injury of an animal according to the present invention is shown. The device comprises a base 1, a placer and a propeller; wherein the placer comprises an outer barrel 2, a planar conical barrel 3, a cylindrical ring 4, a skull fixing nail 5, an occlusion nail 6 and an ultrasonic hole 7; the outer side cylinder 2 is fixedly connected to the upper surface of the base 1, one end of the plane conical cylinder 3 with a large caliber is correspondingly connected to the front end of the outer side cylinder 2, and the cylindrical ring 4 is correspondingly connected to one end of the plane conical cylinder 3 with a small caliber; the skull fixing nails 5 are respectively arranged in the middle of the left side and the right side of the planar conical barrel 3 and on the upper part of the joint of the cylindrical ring 4 and the planar conical barrel 3, the middle of the left side and the right side of the planar conical barrel 3 and the upper part of the joint of the cylindrical ring 4 and the planar conical barrel 3 are all cut with concave grooves for installing the skull fixing nails 5, in the embodiment, three skull fixing nails 5 are totally arranged, rectangular skull clamping sheets 11 are all arranged at the inner ends of the three skull fixing nails 5, the skull clamping sheets 11 positioned on the inner ends of the skull fixing nails 5 on the left side and the right side are respectively parallel to the inner walls of the two sides of the planar conical barrel 3, the skull clamping sheets 11 positioned on the inner ends of the skull fixing nails 5 on the upper part are parallel to the base 1, and the skull clamping sheets 11; the occlusion nail 6 is horizontally arranged in the middle of the joint of the cylindrical ring 4 and the plane conical barrel 3, a concave groove for mounting the occlusion nail 6 is cut in the middle of the joint of the cylindrical ring 4 and the plane conical barrel 3, and the ultrasonic hole 7 is correspondingly arranged in the center of the upper part of the plane conical barrel 3;

the thruster comprises a piston 8, a clamping plate 9 and an adjusting screw 10; piston 8 sets up inside a section of thick bamboo 2 outside, just the diameter of piston 8 is tangent with the inner wall of a section of thick bamboo 2 outside, grip block 9 corresponds the left and right sides that sets up in 2 rear end middle parts of a section of thick bamboo outside and the left and right sides in 8 rear end middle parts of piston respectively, adjusting screw 10 is provided with two, connects respectively between the grip block 9 with one side at 2 and piston 8 outside a section of thick bamboo, the middle part of grip block 9 all is cut with and is used for installing adjusting screw 10 concave groove.

According to the invention, the outer cylinder 2, the plane conical cylinder 3 and the cylindrical ring 4 are used for placing a rat, the piston 8 is driven to move by adjusting the adjusting screw 10 in the propeller to push the experimental rat inwards, the rat cranial cavity and the mouth are pressed and fixed by enabling the mouth of the rat to bite the bite nail 6 and adjusting the skull fixing nail 5 inwards, the ultrasonic hole 7 is positioned at the center of the upper part of the plane conical cylinder 3 and is adaptive to the size of an ultrasonic probe of an ultrasonic therapeutic apparatus, the rat can be fixed in the front-back direction, the left-right direction and the up-down direction along with the pushing of the propeller and the fixing of the skull fixing nail 5 and the bite nail 6, and the ultrasonic probe is placed on the ultrasonic hole 7 to achieve the purpose of ultrasonic therapy.

A fixation method for ultrasound treatment of brain injury in an animal, the method comprising the steps of:

step S1: preparing a moderate traumatic brain injury model of a rat, and carrying out ultrasonic treatment after 24 hours;

step S2: placing the base 1 on a plane, rotating an adjusting screw 10 in the propeller outwards, separating the adjusting screw from the clamping plate 9, and placing the experimental rat into the outer cylinder 2;

step S3: an adjusting screw 10 is arranged between the clamping plates 9 at the two sides, the adjusting screw 10 is rotated inwards, the piston 8 is pushed inwards, the head of the rat sequentially passes through the outer cylinder 2 and the plane conical cylinder 3 inwards, and finally the mouth of the rat reaches the cylindrical ring 4, so that the mouth of the rat bites the biting nail 6;

step S4: rotating skull fixing nails 5 at two sides of a planar conical cylinder 3 in the placer towards the inside of the cylinder until two sides of the head of a rat are clamped, and then rotating the skull fixing nails 5 at the upper part of the planar conical cylinder 3 towards the inside of the cylinder until the mouth of the rat is clamped, so that the brain injury part of the experimental rat is aligned with an ultrasonic hole 7;

step S5: placing the probe of the ultrasonic therapeutic apparatus to the brain injury part through the ultrasonic hole 7, and carrying out ultrasonic therapy on the rat;

step S6: after ultrasonic treatment, rotating the skull fixing nails 5 at the upper part of the planar conical cylinder 3 in the placer towards the outside of the cylinder, then rotating the skull fixing nails 5 at the two sides of the planar conical cylinder 3 towards the outside of the cylinder, enabling the skull clamping sheet 11 at the inner end of the skull fixing nail 5 to be tightly attached to the cylinder wall, rotating the adjusting screw 10 outwards, enabling the piston 8 to be drawn out outwards, separating the mouth of a rat from the occlusion nail 6, and normally feeding;

step S7: two weeks of ultrasound treatment was performed on brain injured rats and experimental data were recorded.

The above-mentioned embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solution of the present invention by those skilled in the art should fall into the protection scope defined by the claims of the present invention without departing from the spirit of the present invention.