阅读说明：本技术 环境污染物质所致损伤的抑制剂、化妆品及饮料食品 (Inhibitor for damage caused by environmental pollutants, cosmetic and food and drink ) 是由 桥井洋子 池冈佐和子 于 2020-10-14 设计创作，主要内容包括：本发明提供环境污染物质所致损伤的抑制剂、化妆品及饮料食品。本发明从安全性高的来自天然物的成分中发现了具有环境污染物质所致损伤的抑制作用的成分,提供一种将该成分作为有效成分的环境污染物质所致损伤的抑制剂、配合有来自该天然物的成分对由于环境污染物质所致损伤的抑制用化妆品以及由于环境污染物质所致损伤的抑制用饮料食品。作为本发明所涉及的环境污染物质所致损伤的抑制剂的有效成分,为阳桃果实提取物。另外,在本发明的环境污染物质所致损伤的抑制用化妆品和环境污染物质所致损伤的抑制用饮料食品中配合阳桃果实提取物。(The invention provides an inhibitor for damage caused by environmental pollutants, a cosmetic, and a food or beverage. The present invention provides a component having an inhibitory effect on damage caused by an environmental pollutant from a highly safe natural-derived component, an agent for inhibiting damage caused by an environmental pollutant containing the component as an active ingredient, a cosmetic for inhibiting damage caused by an environmental pollutant containing the component derived from the natural-derived component, and a food or drink for inhibiting damage caused by an environmental pollutant. The active ingredient of the inhibitor for damage caused by environmental pollutants according to the present invention is an extract of the fruit of Averrhoa carambola. Further, the cosmetic for inhibiting damage caused by environmental pollutants and the food and drink for inhibiting damage caused by environmental pollutants of the present invention contain an extract of the fruit of Averrhoa carambola.)

1. An agent for inhibiting damage caused by environmental pollutants, characterized in that an extract from the fruit of Averrhoa carambola L.

2. The inhibitor for damage by an environmental contaminant according to claim 1, which is used for inhibiting the carbonylation of a protein by an environmental contaminant and/or for inhibiting the increase in the expression of an inflammation-related gene by an environmental contaminant.

3. A cosmetic for inhibiting damage caused by environmental pollutants, which comprises an extract from the fruit of Averrhoa carambola L.

4. A food or beverage for inhibiting damage caused by environmental pollutants, which comprises an extract from the fruit of Averrhoa carambola L.

Technical Field

The present invention relates to an inhibitor of damage caused by environmental pollutants. The present invention also relates to a cosmetic and a food or drink for inhibiting damage caused by an environmental pollutant.

Background

In recent years, attention has been focused on environmental problems such as air pollution and water pollution caused by industrialization. Gaseous pollutants such as sulfur oxides and nitrogen oxides and various environmental pollutants in the form of PM2.5 particles, which are contained in exhaust water and exhaust gas from factories and automobile exhaust gas.

Environmental pollutants cause bronchitis, asthma, cancer, heart disease, destruction of bone or cartilage, fever, reduced water retention of skin, reduced skin barrier function, rough skin, inflammation, etc. by inhalation or attachment to the skin. Among them, it is known that a secondary product containing a mutant (carcinogen) substance is generated by reacting a nitrogen compound with a polycyclic aromatic hydrocarbon which is one of environmental pollutants. It follows that damage to the human body due to environmental pollutants has become an important issue.

Conventionally, known cosmetic compositions useful for protecting skin, scalp, hair and external mucous membrane from contamination with atmospheric pollutants (heavy metals) are oak extract, grape seed extract and green tea extract (patent document 1).

Documents of the prior art

Patent document

Patent document 1 Japanese patent application laid-open No. 2019-510800

Disclosure of Invention

The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a component having an inhibitory effect on damage caused by an environmental pollutant from a highly safe natural product, and to provide an active ingredient of the component as an inhibitor of damage caused by an environmental pollutant, and a cosmetic, a drink or a food containing the component derived from a natural product.

The present inventors have conducted studies to solve the above problems, and as a result, have found that an extract from the fruit of carambola (Averrhoa carambola L.) has an excellent effect of inhibiting damage caused by environmental pollutants, thereby completing the present invention. Specifically, the present invention is as follows.

[ 1] an inhibitor of damage caused by environmental pollutants, characterized in that an extract from the fruit of Averrhoa carambola L.

The use of the inhibitor for inhibiting the carbonylation of a protein by an environmental contaminant and/or the increase in the expression of an inflammation-related gene by an environmental contaminant, according to claim 1.

[ 3] A cosmetic for inhibiting damage caused by environmental pollutants, which comprises an extract from the fruit of Averrhoa carambola L.

[ 4] A food or beverage for inhibiting damage caused by environmental pollutants, which is characterized by comprising an extract from the fruit of Averrhoa carambola L.

According to the present invention, an extract derived from the fruit of Averrhoa carambola L can be used as an active ingredient to provide an agent for inhibiting damage caused by environmental pollutants, which has excellent action and high safety. Furthermore, by blending an extract derived from the fruit of Averrhoa carambola L, it is possible to provide a cosmetic and a food and drink suitable for use in the inhibition of damage caused by environmental pollutants.

Detailed Description

Hereinafter, embodiments of the present invention will be described.

The inhibitor for damage caused by environmental pollutants according to the present embodiment contains an extract from the fruit of carambola as an active ingredient. In addition, the cosmetic and food or beverage of the present embodiment is used for the purpose of suppressing damage caused by environmental pollutants, and contains an extract from the fruit of carambola.

Here, the "extract derived from the fruit of carambola" in the present embodiment includes any of an extract obtained by extraction treatment of the fruit of carambola, a diluted solution or a concentrated solution of the extract, a dried product obtained by drying the extract, or a crude purified product or a purified product thereof.

The extraction material used in the present embodiment is a fruit of Averrhoa carambola L. Carambola belongs to the family oxalis, also known as Ficus quinata and carambola (the name of crude drug), and its fruit is edible. In china, carambola has been described in the literature since the time of the year, and its fruit has a star-shaped cross section, and is called "star fruit" or "carambola". Carambola is grown in okinawa, southeast China, Yunnan, and other tropical areas, and can be easily obtained from these areas.

It is appropriate that the fruit of carambola used as the extraction raw material is dried immediately after collection. The drying can be carried out in the sun or by using a conventional dryer. The fruits of Averrhoa carambola can be dried, pulverized, and crushed. The fruits of Averrhoa carambola can be used as raw materials for extraction by subjecting to pretreatment such as defatting with nonpolar solvent such as hexane. By performing pretreatment such as degreasing, polar solvent extraction treatment of the carambola fruit can be efficiently performed.

The extraction solvent is preferably a polar solvent, and examples thereof include water and a hydrophilic organic solvent, and two or more of these solvents are preferably used alone or in combination at room temperature or at a temperature equal to or lower than the boiling point of the solvent.

Water that can be used as an extraction solvent includes pure water, tap water, well water, mineral water, thermal spring water, gushing water, fresh water, and the like, and water obtained by subjecting these waters to various treatments. Examples of the treatment to be performed on water include purification, heating, sterilization, filtration, ion exchange, osmotic pressure adjustment, and buffering. Therefore, water that can be used as an extraction solvent in the present embodiment also includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate-buffered physiological saline, and the like.

Examples of the hydrophilic organic solvent that can be used as the extraction solvent include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propanol, and isopropanol; lower aliphatic ketones such as acetone and methyl ethyl ketone; and polyhydric alcohols having 2 to 5 carbon atoms such as 1, 3-butanediol, propylene glycol, and glycerin.

When a mixed solution of two or more polar solvents is used as the extraction solvent, the mixing ratio thereof can be appropriately adjusted. For example, when a mixed solution of water and a lower aliphatic alcohol is used as the extraction solvent, the mixing ratio of water and the lower aliphatic alcohol is preferably 9: 1 to 1: 9 (volume ratio), and more preferably 7: 3 to 2: 8 (volume ratio). In addition, in the case of using a mixed liquid of water and a lower aliphatic ketone, the mixing ratio of water and the lower aliphatic ketone is preferably 9: 1 to 2: 8 (volume ratio), and in the case of using a mixed liquid of water and a polyhydric alcohol, the mixing ratio of water and the polyhydric alcohol is preferably 8: 2 to 1: 9 (volume ratio).

The extraction treatment is not particularly limited as long as it can elute soluble components contained in the extraction raw material into the extraction solvent, and can be carried out by a conventional method. For example, the extraction solution can be obtained by immersing the extraction raw material in an extraction solvent in an amount (mass ratio) 5 to 15 times that of the extraction raw material, extracting soluble components at room temperature or under reflux heating, and then filtering to remove the extraction residue. The solvent was distilled off from the obtained extract to obtain a paste-like concentrate, which was further dried to obtain a dried product.

< inhibitors of damage caused by environmental pollutants >

The extract derived from the fruit of carambola obtained as described above (hereinafter, may be referred to simply as "carambola fruit extract") has an excellent effect of inhibiting damage caused by environmental pollutants, and therefore can be used as an active ingredient of an inhibitor of damage caused by environmental pollutants. The inhibitor for damage caused by an environmental pollutant according to the present embodiment can be widely used in pharmaceutical products, quasi-pharmaceutical products, cosmetics, and the like. When the inhibitor of damage caused by environmental pollutants according to the present embodiment is simply referred to as "damage inhibitor", the same applies to the action and use thereof.

Examples of the environmental pollutants include water pollutants (e.g., alkylbenzene sulfonates), soil pollutants (e.g., agricultural chemicals), and air pollutants (e.g., exhaust gas from gasoline engines and tunnel dust), but are not limited thereto. From the viewpoint of more effectively suppressing damage, air pollutants (exhaust gas from gasoline engines, tunnel dust, and the like) may be mentioned.

Here, the damage-inhibiting effect of the extract of the carambola fruit is exerted, for example, based on an inhibitory effect on the carbonylation of a protein by an environmental pollutant or an inhibitory effect on the increase in the expression of an inflammation-related gene by an environmental pollutant. However, the damage-inhibiting action of the extract of the fruit of Averrhoa carambola is not limited to the damage-inhibiting action based on the protein carbonylation-inhibiting action or the inflammation-related gene expression-increasing-inhibiting action.

Examples of the site of the extract of the fruit of Averrhoa carambola which inhibits protein carbonylation induced by environmental pollutants include skin (including scalp), bronchus, blood vessels, and hair, and particularly, the skin includes epidermis and dermis, but from the viewpoint of more effectively inhibiting protein carbonylation, the epidermis may also include horny layer.

The genes of Averrhoa carambola fruit extract which inhibit the over-expression of the inflammation-related genes caused by environmental pollutants include interleukin 1 alpha (IL-1 alpha), cyclooxygenase 2(COX-2), and matrix metalloproteinase 9 (MMP-9).

Examples of the site where the extract of the fruit of Averrhoa carambola is effective in inhibiting the overexpression of the inflammation-related gene due to environmental pollutants include the skin (including scalp), bronchus, and blood vessels, and particularly, the skin includes the epidermis and dermis, and from the viewpoint of more effectively inhibiting the expression of the inflammation-related gene, the epidermis may also include epidermal keratinocytes.

By utilizing the inhibitory action of the extract of the fruit of Averrhoa carambola on the carbonylation of a protein by an environmental pollutant or the increase in the expression of an inflammation-related gene by an environmental pollutant, particularly the inhibitory action on the increase in the expression of IL-1. alpha. by an environmental pollutant, the inhibitory action on the increase in the expression of COX-2 by an environmental pollutant or the inhibitory action on the increase in the expression of MMP-9 by an environmental pollutant, can be used as an active ingredient such as an inhibitor of protein carbonylation caused by an environmental contaminant, an inhibitor of increase in IL-1. alpha. expression caused by an environmental contaminant, an inhibitor of increase in COX-2 expression caused by an environmental contaminant, or an inhibitor of increase in MMP-9 expression caused by an environmental contaminant.

The damage inhibitor of the present embodiment may be composed of a single extract of the kiwi fruit, or the extract of the kiwi fruit may be formulated.

The damage inhibitor of the present embodiment can be formulated into any dosage form such as powder, granule, tablet, liquid, etc. by a conventional method using a pharmaceutically acceptable carrier such as dextrin or cyclodextrin and any other auxiliary agent. In this case, as the auxiliary, for example, an excipient, a binder, a disintegrating agent, a lubricant, a stabilizer, a flavoring agent, a deodorizing agent, and the like can be used. The damage inhibitor can be used in combination with other compositions (e.g., cosmetics), and can also be used as an ointment, a liquid preparation for external use, a patch, or the like.

When the damage inhibitor of the present embodiment is formulated, the content of the extract of the carambola fruit is not particularly limited, and may be appropriately set according to the purpose.

The damage-suppressing agent of the present embodiment may be used as an active ingredient by blending, as needed, another natural extract having a damage-suppressing effect or the like with the extract of the fruit of carambola.

Examples of the method of administering the damage inhibitor of the present embodiment to a patient include transdermal administration, oral administration, and the like, and a method suitable for the prevention, treatment, and the like of a disease may be appropriately selected depending on the type of the disease. The dose of the damage inhibitor of the present embodiment may be increased or decreased as appropriate depending on the type and severity of the disease, individual differences of patients, administration method, administration time, and the like.

The damage inhibitor of the present embodiment inhibits damage caused by environmental pollutants by the action of an extract of the fruit of Averrhoa carambola as an active ingredient. The damage inhibitor of the present embodiment is an inhibitory effect exerted by the extract of the fruit of carambola on the prevention, treatment, or improvement of various symptoms caused by environmental pollutants (for example, bronchitis, asthma, canceration, heart disease, destruction of bone or cartilage, fever, reduction in skin water retention, reduction in skin barrier function, skin roughness, inflammation, hair damage, and the like). .

However, the use of the damage inhibitor of the present embodiment can be used for all purposes that act to inhibit damage caused by environmental pollutants, in addition to the use thereof. For example, the damage inhibitor or the above-mentioned inhibitor of protein carbonylation by environmental pollutants can inhibit protein carbonylation by environmental pollutants by the action of the extract of the fruit of Averrhoa carambola as an active ingredient. The damage inhibitor of the present embodiment inhibits protein carbonylation due to environmental pollutants by the action of the extract of the fruit of Averrhoa carambola, and prevents, treats or improves symptoms such as canceration, fever, reduction in skin water retention, reduction in skin barrier function, skin roughness, inflammation, and hair damage.

For example, the damage inhibitor of the present embodiment, the inhibitor of the increase in the expression of IL-1. alpha. caused by the environmental pollutants, the inhibitor of the increase in the expression of COX-2 caused by the environmental pollutants, or the inhibitor of the increase in the expression of MMP-9 caused by the environmental pollutants, is an inhibitor of the increase in the expression of genes involved in inflammation caused by the environmental pollutants, due to the action of the extract of Averrhoa carambola fruit as an active ingredient. The inflammation-related genes include IL-1 alpha, COX-2, MMP-9, and the like. The damage inhibitor of the present embodiment inhibits an increase in expression of an inflammation-related gene caused by an environmental pollutant by the action of the extract of the fruit of Averrhoa carambola, and prevents, treats or improves symptoms such as canceration, destruction of bone or cartilage, fever, reduction in skin water retention, reduction in skin barrier function, skin roughness, and inflammation.

The damage-suppressing agent of the present embodiment has an excellent effect of suppressing damage, and is therefore suitable for use in combination with preparations such as external preparations for skin and scalp. In this case, the extract of the fruit of Averrhoa carambola can be directly added, or a damage inhibitor prepared from the extract of Averrhoa carambola can be prepared.

Here, the external preparation for percutaneous use is not limited in its classification, and includes quasi-drugs and drugs for percutaneous use, in addition to cosmetics described later.

Further, the damage inhibitor of the present embodiment has an excellent damage-inhibiting effect, and therefore can be used as a reagent for studying the mechanism of such an action.

[ A cosmetic capable of inhibiting damage caused by environmental pollutants ] the excellent effect of inhibiting damage based on the extract of the fruit of Averrhoa carambola is suitably incorporated in cosmetics. In this case, the extract of the fruit of Averrhoa carambola can be directly added, or the damage inhibitor prepared from the extract of Averrhoa carambola can be added.

By compounding with the extract of the fruit of Averrhoa carambola or the above-mentioned damage-inhibiting agent, a cosmetic suitable for damage-inhibiting use can be prepared.

The type of cosmetic preparation prepared from the extract of the fruit of Averrhoa carambola or the above-mentioned damage inhibitor is not particularly limited, and examples thereof include skin cosmetics, hair cosmetics, and the like, and ointments, creams, lotions, gels, beauty oils, face masks, foundations, hair tonics, shampoos, hair creams, hair conditioners, shampoos, hair rinses, hair tonics, and care agents.

When a cosmetic is prepared using the extract of the fruit of Averrhoa carambola or the above-mentioned damage inhibitor, the amount of the extract can be appropriately adjusted depending on the type of the cosmetic, and the preferable blending ratio is about 0.0001 to 10% by mass, and the particularly preferable blending ratio is about 0.001 to 1% by mass in terms of the standard extract.

The cosmetic for damage suppression of the present embodiment can be used in combination as a main agent, an auxiliary agent, or other components of a general cosmetic as long as the damage suppression effect of the extract of the kiwi fruit is not impaired, and examples thereof include an astringent, a bactericidal/antibacterial agent, a whitening agent, an ultraviolet absorber, a moisturizing agent, a cell activator, an anti-inflammatory/antiallergic agent, an antioxidant/active oxygen removing agent, oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, a surfactant, and a perfume. By such a combination, a more general product can be obtained, and further, a better effect than expected can be produced by a synergistic effect with other active ingredients.

The damage-suppressing cosmetic of the present embodiment can prevent, treat, or improve symptoms caused by environmental pollutants (for example, reduction in skin water retention, reduction in skin barrier function, skin roughness, inflammation, hair damage, and the like) by the damage-suppressing action of the extract of the fruit of aversion to carambola.

[ A beverage or food capable of suppressing damage caused by environmental pollutants ]

The extract is suitable for blending in food and drink, because of its excellent effect in inhibiting the damage. In this case, the extract of the fruit of Averrhoa carambola can be directly added, or a damage inhibitor prepared from the extract of Averrhoa carambola can be added. By compounding with the extract of the fruit of Averrhoa carambola or the above damage inhibitor, a food or drink suitable for damage-inhibiting use can be prepared.

Here, the term "food and drink" refers to a substance that has a low possibility of harming human health and is taken by oral or digestive administration in general social life, and is not limited to the division of foods, medicines, quasi-medicines, and the like in the administrative division. Therefore, the "food and drink" in the present embodiment broadly includes general foods, health foods (functional foods and drinks), health functional foods (specific health foods, nutritional functional foods), quasi drugs, and the like which are orally ingested. The food and drink according to the present embodiment is preferably a food and drink capable of exhibiting the advantageous effects of the extract from the fruit of carambola on the food and drink or a package thereof, and particularly preferably a health-care functional food (specific health-care food, functional display food, nutritional functional food), a quasi-drug, and a drug.

When the extract of the Averrhoa carambola fruit or the damage inhibitor formulated from the extract of the Averrhoa carambola fruit is incorporated into a food or drink, the amount of the active ingredient incorporated in the extract can be appropriately changed depending on the purpose of use, symptoms, sex, etc., but considering the general intake amount of the food or drink to be added, it is preferable that the daily intake amount of the extract for an adult is about 1 to 1000 mg. In the case where the food or beverage to be added is a granular, tablet, or capsule food or beverage, the amount of the extract of the carambola fruit or the damage inhibitor formulated from the extract of the carambola fruit is usually 0.1 to 100% by mass, preferably 5 to 100% by mass, based on the food or beverage to be added.

The damage-suppressing food and drink of the present embodiment may be any food and drink in which the extract of the fruit of Averrhoa carambola is blended in a state that does not inhibit the activity thereof, or may be a nutritional supplement containing the extract of the fruit of Averrhoa carambola as a main component.

In the production of the food or beverage for preventing damage according to the present embodiment, for example, sugars such as dextrin and starch; proteins such as gelatin, soybean protein, and zein; amino acids such as alanine, glutamine, and isoleucine; polysaccharides such as cellulose and gum arabic; soybean oil, medium-chain fatty acid triglyceride and other oil and fat, and making into beverage and food with any shape.

The beverage or food to be blended with the extract of the fruit of Averrhoa carambola is not particularly limited, and specific examples thereof include: beverages (including concentrated raw liquids and powders for adjustment thereof) such as refreshing beverages, carbonated beverages, nutritional beverages, fruit beverages, and lactic acid beverages; freezing and coring ice cream, sherbet, water ice, etc.; buckwheat flour, udon flour, vermicelli, dumpling wrapper, steamed dumpling wrapper, Chinese noodles, instant noodles, etc.; confectionery such as maltose, chewing gum, candy, chewing gum, chocolate, candy, snack, biscuit, jelly, jam, cream, baked snack, etc.; processed food of aquatic products/livestock products such as fish cake, ham, sausage, etc.; processed milk, fermented milk, and other dairy products; salad oil, frying oil, margarine, mayonnaise, shortening, whipped cream, salad sauce, etc. and processed food of oil; sauce, seasoning juice, etc.; soup, stew, salad, home dish, pickled product; other health/nutritional supplements in various forms; tablets, capsules, energy drinks, and the like. When the extract of the carambola fruit is added to these foods and beverages, usual auxiliary materials or additives may be used in combination.

The damage-suppressing food and beverage according to the present embodiment can prevent, treat, or ameliorate symptoms caused by environmental pollutants (for example, bronchitis, asthma, cancer, heart disease, bone or cartilage destruction, fever, reduction in skin water retention, reduction in skin barrier function, skin roughness, inflammation, hair damage, and the like) by the damage-suppressing effect of the extract of the carambola fruit.

The damage-suppressing agent, the damage-suppressing cosmetic, and the damage-suppressing food and drink according to the present embodiment are suitably used for humans, but may be applied to animals other than humans (for example, mice, rats, hamsters, dogs, cats, cows, pigs, monkeys, etc.) as long as they can exhibit their respective effects.

Examples

The present invention will be specifically described below by way of examples, but the present invention is not limited to the following examples.

[ production example ]

100g of a roughly pulverized product of a fruit of Averrhoa carambola L. is put into 1500ml of an extraction solvent, and extracted at 45 to 55 ℃ for 4 hours while gently stirring. Filtering, concentrating the filtrate at 60 deg.C under reduced pressure, and further drying with vacuum drier to obtain extract of Averrhoa carambola fruit. The yield of the extract is shown in table 1.

[ TABLE 1]

Extraction solvent	Yield of extract (% by mass)
		60% ethanol	10.875

[ test example 1] inhibitory Effect on protein carbonylation by environmental pollutants

The carambola fruit extract (sample 1) was tested for its inhibitory effect on the carbonylation of stratum corneum proteins by environmental pollutants as follows.

The horny layer on the inner side of the upper arm, which is a non-exposed part, was peeled and collected by a tape peeling method using a horny substance tester (manufactured by ASAHI BIOMED corporation). A cutin tester (sample) having a cutin layer adhered thereto was immersed in 1mL of an aqueous solution of a test sample (sample 1, sample concentration shown in Table 2 below) and treated at 37 ℃ for 24 hours. As a control, water to which no sample was added was similarly treated. After treatment, the samples were removed and air dried. Then, it was exposed to exhaust gas from a gasoline engine collected in a plastic bag with a zipper and reacted at room temperature for 24 hours. As a blank, a sample exposed to room air was also prepared. The level of horny layer carbonylation of the obtained samples was evaluated by the following method.

The samples were immersed in 0.1mol/L MES buffer (pH5.5) containing 20. mu. mol/L fluorohydrazide (fluorescein-5-thiosemicarbazide, manufactured by Anaspec corporation), and they were reacted for 1 hour under a light shielding condition at room temperature to tag the carbonyl group of the stratum corneum protein. After completion of the reaction, the reaction mixture was washed 2 times with 1mL of PBS (-) buffer, and the image was photographed by a fluorescence microscope (BZ-X800, manufactured by Kinzhi). The obtained image was analyzed using image analysis software (BZ-X800 analyzer, manufactured by keyence corporation), and the fluorescence intensity per unit area of the horny layer was used as the carbonylation level. From the obtained results, the horny layer protein carbonylation inhibition ratio (%) was calculated by the following formula.

Horny layer protein carbonylation inhibition ratio (%) {1- (a-C)/(B-C) } × 100

The terms in the formula represent the following values, respectively:

a: carbonylation level of exhaust gas treatment and treatment of sample to be tested

B: carbonylation level of exhaust treatment, treatment without test sample (control)

C: carbonylation level without waste gas treatment, without treatment of the sample to be tested (blank)

The results are shown in Table 2.

[ TABLE 2]

As shown in table 2, the extract of the fruit of carambola (sample 1) effectively inhibited the carbonylation of stratum corneum proteins caused by off-gas.

[ test example 2] inhibitory Effect on the increase in IL-1. alpha. expression caused by environmental pollutants

The carambola fruit extract (sample 1) was tested for its inhibitory effect on the increase in the expression of IL-1 α caused by environmental pollutants as follows.

After normal human epidermal keratinocytes were cultured in a proliferation medium (KGM) for normal human neonatal epidermal keratinocytes (NHEK), the cells were treated with trypsin and recovered. The recovered cells were diluted with KGM medium to a cell density of 20X 10⁴cells/mL, then inoculated into 35mm petri dishes, 2mL (40X 10) per dish⁴cells/petri dish) for 24 hours.

After the culture, the medium was replaced with a basal medium (KBM) and the culture was allowed to stand for 24 hours, and then the culture solution was removed, and a sample to be tested (sample 1, sample concentration shown in Table 3 below) and tunnel dust for polycyclic aromatic hydrocarbons/hazardous elements analysis (institute for Integrated Industrial technology; certified Standard substance NMIJCRM 7308-a, final concentration 20. mu.g/mL) were dissolved in the KBM medium, and the medium was added to each dish, and the culture was allowed to stand for 24 hours at a rate of 2mL per dish. As a control, the KBM medium to which no tunnel dust was added or the KBM medium to which no test sample was added was used for the culture in the same manner. After the culture, the medium was removed, and total RNA was extracted with ISOGEN II (NIPPONGENE Co., Ltd., Cat. No.311-07361), and the amount of each RNA was measured with a spectrophotometer to obtain total RNA at a concentration of 200 ng/. mu.L.

The total RNA was used as a template, and the expression level of mRNA was measured for IL-1. alpha. and GAPDH as an internal standard. The detection was carried out by a Real-Time two-step RT-PCR reaction using a Real-Time PCR apparatus Smart Cycler (manufactured by Cepheid Co., Ltd.) and a TaKaRa SYBR PrimeScript RT-PCR kit (Perfect read Time) (manufactured by TAKARA Bio Inc., code No. RR063A). The expression level of IL-1. alpha. mRNA was corrected by the GAPDH value based on the total RNA standards prepared from the cells cultured in "no tunnel dust added, no test sample added", "tunnel dust added, no test sample added" and "tunnel dust added, test sample added". From the obtained values, the inhibition rate (%) of the increase in IL-1. alpha. mRNA expression was calculated by the following formula.

Each of the inhibition rates (%) for the increase in IL-1 α mRNA expression { (a-B) - (a-C) }/(a-B) × 100 represents the following value, respectively:

a: correction value without tunnel dust and without sample to be measured

B: correction value when tunnel dust is added and sample to be measured is not added

C: correction value for adding tunnel dust and adding sample to be measured

The results are shown in Table 3.

[ TABLE 3]

As shown in Table 3, it was confirmed that the Averrhoa carambola fruit extract (sample 1) has an excellent effect as an inhibitory effect on the increase in IL-1. alpha. expression caused by environmental pollutants.

[ test example 3] inhibitory Effect on COX-2 expression increase caused by environmental pollutants

The carambola fruit extract (sample 1) was tested for its inhibitory effect on the increase in COX-2 expression caused by environmental pollutants as follows.

After normal human epidermal keratinocytes were cultured in a proliferation medium (KGM) for normal human neonatal epidermal keratinocytes (NHEK), the cells were treated with trypsin and recovered. The recovered cells were diluted with KGM medium to a cell density of20×10⁴cells/mL, then inoculated into 35mm petri dishes, 2mL (40X 10) per dish⁴cells/petri dish) for 24 hours.

After the culture, the medium was replaced with a basal medium (KBM) and the culture was allowed to stand for 24 hours, and then the culture solution was removed, and a sample to be tested (sample 1, sample concentration shown in Table 4 below) and tunnel dust for analysis of polycyclic aromatic hydrocarbons/hazardous elements (institute for Integrated Industrial technology; certified Standard substance NMIJCRM 7308-a, final concentration 20. mu.g/mL) were dissolved in the KBM medium, and 2mL of the KBM medium was added to each dish and cultured for 24 hours. As a control, KBM medium without addition of tunnel dust or KBM medium without addition of a sample was used for the culture in the same manner. After the culture, the medium was removed, and total RNA was extracted with ISOGEN II (NIPPONGENE Co., Ltd., Cat. No.311-07361), and the amount of each RNA was measured with a spectrophotometer to obtain total RNA at a concentration of 200 ng/. mu.L.

The total RNA was used as a template, and the expression level of mRNA was measured for COX-2 and GAPDH as an internal standard. The detection was carried out by a Real-Time two-step RT-PCR reaction using a Real-Time PCR apparatus Smart Cycler (manufactured by Cepheid Co., Ltd.) and a TaKaRa SYBR PrimeScript RT-PCR kit (Perfect read Time) (manufactured by TAKARA Bio Inc., code No. RR063A). The expression level of COX-2mRNA was corrected by the GAPDH value based on the total RNA standard prepared from cells cultured in "no tunnel dust added, no test sample added", "tunnel dust added, no test sample added" and "tunnel dust added, test sample added". From the obtained values, the inhibition rate (%) of COX-2mRNA expression increase was calculated by the following formula.

COX-2mRNA expression increase inhibition rate (%) { (a-B) - (a-C) }/(a-B) × 100

The terms in the formula represent the following values, respectively:

a: correction value without tunnel dust and without sample to be measured

B: correction value when tunnel dust is added and sample to be measured is not added

C: correction value for adding tunnel dust and adding sample to be measured

The results are shown in Table 4.

[ TABLE 4]

As shown in Table 4, it was confirmed that the extract of Averrhoa carambola fruit (sample 1) has an excellent effect as an inhibitory effect on the increase of COX-2 expression caused by environmental pollutants.

[ test example 4] inhibitory Effect on the increase in MMP-9 expression caused by environmental pollutants

The carambola fruit extract (sample 1) was tested for its inhibitory effect on the increase in MMP-9 expression caused by environmental pollutants as follows.

After normal human epidermal keratinocytes were cultured in a proliferation medium (KGM) for normal human neonatal epidermal keratinocytes (NHEK), the cells were treated with trypsin and recovered. The recovered cells were diluted with KGM medium to a cell density of 20X 10⁴cells/mL, then inoculated into 35mm petri dishes, 2mL (40X 10) per dish⁴cells/petri dish) for 24 hours.

After the culture, the medium was replaced with a basal medium (KBM) and the culture was carried out for 24 hours, then the culture solution was removed, and a KBM medium containing a sample to be tested (sample 1, sample concentration shown in Table 5 below) and tunnel dust for analysis of polycyclic aromatic hydrocarbons and harmful elements (institute for Integrated Industrial technology; certified Standard substance NMIJCRM 7308-a, final concentration 20. mu.g/mL) was added to each dish and cultured for 24 hours while adding 2mL to each dish. As a control, KBM medium without addition of tunnel dust or KBM medium without addition of a sample was used for the culture in the same manner. After the culture, the medium was removed, and total RNA was extracted with ISOGEN II (NIPPONGENE Co., Ltd., Cat. No.311-07361), and the amount of each RNA was measured with a spectrophotometer to obtain total RNA at a concentration of 200 ng/. mu.L.

The total RNA was used as a template, and the expression level of mRNA was measured for MMP-9 and GAPDH as an internal standard. The detection was carried out by a Real-Time two-step RT-PCR reaction using a Real-Time PCR apparatus Smart Cycler (manufactured by Cepheid Co., Ltd.) and a TaKaRa SYBR PrimeScript RT-PCR kit (Perfect read Time) (manufactured by TAKARA Bio Inc., code No. RR063A). The expression level of MMP-9 mRNA was corrected by the GAPDH value based on the total RNA standard prepared from the cells cultured in "no tunnel dust added, no test sample added", "tunnel dust added, no test sample added" and "tunnel dust added, test sample added". From the obtained values, the MMP-9 mRNA expression increase inhibition rate (%) was calculated by the following formula.

MMP-9 mRNA expression increase inhibition rate (%) { (a-B) - (a-C) }/(a-B) × 100

The terms in the formula represent the following values, respectively:

a: correction value without tunnel dust and without sample to be measured

B: correction value when tunnel dust is added and sample to be measured is not added

C: correction value for adding tunnel dust and adding sample to be measured

The results are shown in Table 5.

[ TABLE 5 ]

As shown in Table 5, it was confirmed that the Averrhoa carambola fruit extract (sample 1) has an excellent effect as an inhibitory effect on the increase in MMP-9 expression caused by environmental pollutants.

[ blending example 1]

An emulsion was produced by a conventional method according to the following composition.

[ working example 2]

A cream having the following composition was produced by a conventional method.

[ working example 3]

A cosmetic liquid having the following composition was produced by a conventional method.

[ working example 4]

The hair conditioner having the following composition was produced by a conventional method.

[ working example 5 ]

Tablets having the following composition were manufactured by a conventional method.

[ working example 6 ]

An oral liquid preparation having the following composition was manufactured by a conventional method.

< composition in ampoule (1 100mL) >

The inhibitor for damage by an environmental pollutant, the cosmetic for inhibiting damage by an environmental pollutant, and the food or drink for inhibiting damage by an environmental pollutant according to the present invention can contribute greatly to the prevention, treatment, or amelioration of various symptoms (for example, bronchitis, asthma, canceration, heart disease, bone or cartilage destruction, fever, reduction in skin water retention, reduction in skin barrier function, skin roughness, inflammation, hair damage, and the like) caused by an environmental pollutant.