阅读说明：本技术 一种基于胶原蛋白提取循环盐析滴加装置 (Circulating salting-out dropwise adding device based on collagen extraction ) 是由 曾兴东 于 2021-01-19 设计创作，主要内容包括：本发明提供一种基于胶原蛋白提取循环盐析滴加装置,涉及胶原蛋白提取技术领域,解决了胶原蛋白提取盐析滴加时,由于胶原粘度比较大,NaCl加入胶原溶液中后不能迅速均匀的分散开来,导致胶原析出的不均匀,以及在析出的同时会裹带部分未溶解的NaCl下来,导致胶原提取效果较差的问题。一种基于胶原蛋白提取循环盐析滴加装置,包括盐析罐,所述盐析罐底部安装有与其内腔相连通的循环管道。因第四曝气管微孔部位以气泡形式弥散逸出气流将翻搅盐析罐内腔底面的沉淀物,故处于翻搅状态下的溶液经由正玄泵从排出管抽取至循环管道内,然后从与盐析罐连通部位重新输入至盐析罐内,实现盐析罐内溶液的循环,进一步提高盐析罐内的所滴加溶液的混匀效率。(The invention provides a cyclic salting-out dripping device based on collagen extraction, relates to the technical field of collagen extraction, and solves the problems that when the collagen extraction is subjected to salting-out dripping, due to the fact that the viscosity of collagen is high, NaCl cannot be rapidly and uniformly dispersed after being added into a collagen solution, the collagen precipitation is not uniform, and the collagen extraction effect is poor due to the fact that NaCl which is not dissolved is wrapped by the NaCl when the NaCl is precipitated. A circulating salting-out dripping device based on collagen extraction comprises a salting-out tank, wherein a circulating pipeline communicated with an inner cavity of the salting-out tank is arranged at the bottom of the salting-out tank. Because the microporous part of the fourth aeration pipe disperses the escaping airflow in the form of bubbles to stir the sediment on the bottom surface of the inner cavity of the salting-out tank, the solution in the stirring state is pumped into the circulating pipeline from the discharge pipe through the positive-pressure pump and then is input into the salting-out tank again from the part communicated with the salting-out tank, the circulation of the solution in the salting-out tank is realized, and the uniform mixing efficiency of the dropwise added solution in the salting-out tank is further improved.)

1. A circulating salting-out dripping device based on collagen extraction is characterized in that: the salting-out device comprises a salting-out tank (1), wherein a circulating pipeline (2) communicated with an inner cavity of the salting-out tank (1) is arranged at the bottom of the salting-out tank (1), and a non-contact stirring mechanism (4) is arranged in the salting-out tank (1); the salting tank (1) comprises an outer heating layer (101) and a tank cover (102), the tank cover (102) is arranged on the top flange of the salting tank (1) in a sealing mode, and the outer heating layer (101) is arranged on the outer peripheral surface of the salting tank (1); the top of the tank cover (102) is provided with a dropping pipe (3) communicated with the inner cavity of the tank cover.

2. The cyclic salting-out and dropping device based on collagen extraction of claim 1, wherein: salting out jar (1) is including PH meter (103), temperature measurement meter (104) and discharge pipe (105), and PH meter (103) and temperature measurement meter (104) are installed respectively to both ends about cover (102), and PH meter (103) and temperature measurement meter (104) measuring end all are located salting out jar (1) inner chamber bottom but not contact rather than inner chamber face, and salting out jar (1) bottom end face is provided with discharge pipe (105) that are linked together rather than the inner chamber.

3. The cyclic salting-out and dropping device based on collagen extraction of claim 1, wherein: the salting-out tank (1) comprises a cylinder (106), a disc (107) and a pressure relief through hole (108), wherein the cylinder (106) is arranged at the right end of the tank cover (102), the disc (107) with the same axis is arranged on the top end face of the cylinder (106), the diameter of the disc (107) is larger than that of the cylinder (106), the pressure relief through hole (108) penetrating through the bottom end face of the cylinder (106) is formed in the axis position of the top end face of the disc (107), and the pressure relief through hole (108) is communicated with the inner cavity of the tank cover (102).

4. The cyclic salting-out and dropping device based on collagen extraction of claim 1, wherein: the salting-out tank (1) comprises a rubber sheet (109) and a cut-off opening (1010), the top end face of the disc (107) is provided with the rubber sheet (109) with the same diameter, the axis part of the rubber sheet (109) is provided with the cut-off opening (1010), the cut-off opening (1010) is of a cross structure, the size of the cut-off opening (1010) is larger than that of the pressure relief through hole (108), and the cut-off surface of the cut-off opening (1010) is in a sealed closed state in a common state.

5. The cyclic salting-out and dropping device based on collagen extraction of claim 1, wherein: the circulating pipeline (2) comprises a positive and negative pump (201) and an electromagnetic valve (202), the head end of the circulating pipeline (2) is fixedly connected with the discharge pipe (105), the electromagnetic valve (202) is installed at the head end of the circulating pipeline (2), the tail end of the circulating pipeline (2) is connected with the top end part of the outer peripheral surface of the salting-out tank (1), the circulating pipeline (2) is communicated with the inner cavity of the salting-out tank (1) in a circulating mode, and the positive and negative pump (201) is installed on the circulating pipeline (2).

6. The cyclic salting-out and dropping device based on collagen extraction of claim 1, wherein: drop tube (3) including flowmeter (301), flow rate control valve (302), extension pipe (303), ring flange (304), sealed lid of flange (305) and dropwise add jar (306), flowmeter (301) and flow rate control valve (302) are installed in proper order to drop tube (3) lower half, drop tube (3) first half periphery is personally submitted the symmetry and is provided with two extension pipes (303) rather than being linked together, two extension pipe (303) tops and drop tube (3) top all are provided with ring flange (304), dropwise add jar (306) are installed through ring flange (304) flange seal in drop tube (3) top, sealed lid of flange (305) is installed through ring flange (304) flange seal in two extension pipe (303) tops.

7. The cyclic salting-out and dropping device based on collagen extraction of claim 1, wherein: the non-contact stirring mechanism (4) comprises a first aeration pipe (401), a second aeration pipe (402), a third aeration pipe (403), a fourth aeration pipe (404), a communication pipeline (405) and an external communication pipeline (406), wherein the first aeration pipe (401) is vertically arranged at the middle part inside the salting-out tank (1), the second aeration pipe (402) and the third aeration pipe (403) are respectively vertically arranged at the inner parts of the salting-out tank (1) at the right side and the left side of the first aeration pipe (401), the fourth aeration pipe (404) is horizontally arranged at the adjacent discharge pipe (105) part at the middle inside the salting-out tank (1), the first aeration pipe (401), the second aeration pipe (402), the third aeration pipe (403) and the fourth aeration pipe (404) are communicated through the communication pipeline (405), and one side of the communication pipeline (405) is communicated with the external communication pipeline (406), the external connecting pipeline (406) is connected with an external airflow input device.

Technical Field

The invention belongs to the technical field of collagen extraction, and particularly relates to a circulating salting-out dripping device based on collagen extraction.

Background

Proproteins are important functional substances constituting animal bodies and have incomparable biocompatibility with other synthetic polymer materials, and thus have increasingly prominent importance as biomaterials in the industries of medicines, foods, cosmetics, and the like.

The collagen extraction process is mainly divided into an acid method, an alkaline method and an enzyme method. The three extraction methods involve a salting-out step, and conventionally NaCl is added to a collagen solution to precipitate the collagen.

For example, application No.: 201310320162.9 the invention discloses a device special for extracting collagen type II as the main component of a natural cartilage matrix, which comprises a constant temperature box, a centrifugal device arranged in the constant temperature box and a control system used for controlling the temperature in the constant temperature box and the action of the centrifugal device, wherein the centrifugal device comprises a centrifugal machine, a container device arranged on the centrifugal machine, a liquid adding system used for adding a reagent into the container device, a filtering and discharging system used for discharging waste liquid in the container device and a stirring system used for stirring the reagent. The special device for extracting the main component of the natural cartilage matrix, namely the type II collagen can automatically extract the main component of the natural cartilage matrix, namely the type II collagen, so that the labor can be effectively saved, the manufacturing cost can be reduced, and the production efficiency can be improved.

Based on the search of the above patent and the discovery of combining the equipment in the prior art, when the collagen is extracted and salted out and dripped, the viscosity of the collagen is relatively high, and NaCl cannot be rapidly and uniformly dispersed after being added into the collagen solution, so that the collagen is not uniformly precipitated, and the insoluble NaCl can be wrapped by the precipitated NaCl, so that the collagen extraction effect is poor.

Disclosure of Invention

In order to solve the technical problems, the invention provides a circular salting-out dripping device based on collagen extraction, which aims to solve the problems that when the collagen extraction is subjected to salting-out dripping, due to the fact that the viscosity of collagen is high, NaCl cannot be rapidly and uniformly dispersed after being added into a collagen solution, the collagen precipitation is not uniform, and the collagen extraction effect is poor due to the fact that NaCl which is not dissolved is wrapped by the NaCl when the NaCl is precipitated.

The invention relates to a purpose and an effect of a circular salting-out dripping device based on collagen extraction, which are achieved by the following specific technical means:

a cyclic salting-out dripping device based on collagen extraction comprises a salting-out tank, wherein a circulating pipeline communicated with an inner cavity of the salting-out tank is arranged at the bottom of the salting-out tank, and a non-contact stirring mechanism is arranged in the salting-out tank; the salting-out tank comprises an outer heating layer and a tank cover, the tank cover is arranged on the top of the salting-out tank in a flange sealing mode, and the outer heating layer is arranged on the peripheral surface of the salting-out tank; and a dropping pipe communicated with the inner cavity of the tank cover is arranged at the top of the tank cover.

Further, the salting-out tank comprises a PH measuring meter, a temperature measuring meter and a discharge pipe, the PH measuring meter and the temperature measuring meter are respectively installed at the left end and the right end of the tank cover, the measuring ends of the PH measuring meter and the temperature measuring meter are both located at the bottom of the inner cavity of the salting-out tank and are not in contact with the inner cavity surface of the salting-out tank, and the discharge pipe communicated with the inner cavity of the salting-out tank is arranged on the end surface of the bottom.

Further, the salting-out tank comprises a cylinder, a disc and a pressure relief through hole, the right end of the tank cover is provided with the cylinder, the top end face of the cylinder is provided with the disc which is coaxial with the cylinder, the diameter of the disc is larger than that of the cylinder, the axle center of the top end face of the disc is provided with the pressure relief through hole which penetrates through the bottom end face of the cylinder, and the pressure relief through hole is communicated with the inner cavity of the tank cover.

Furthermore, the salting-out tank comprises a rubber sheet and a cutting opening, the top end face of the disc is provided with the rubber sheet with the diameter consistent with that of the disc, the axis part of the rubber sheet is provided with the cutting opening, the cutting opening is of a cross structure, the size of the cutting opening is larger than that of the pressure relief through hole, and the cutting surface of the cutting opening is in a sealed closed state under the ordinary state of the cutting opening.

Furthermore, the circulating pipeline comprises a positive pump and an electromagnetic valve, the head end of the circulating pipeline is fixedly connected with the discharge pipe, the electromagnetic valve is installed at the head end of the circulating pipeline, the tail end of the circulating pipeline is connected with the top end part of the outer circumferential surface of the salting-out tank, the circulating pipeline is circularly communicated with the inner cavity of the salting-out tank, and the positive pump is installed on the circulating pipeline.

Further, the dropping pipe is including flowmeter, flow rate control valve, extension pipe, ring flange, the sealed lid of flange and dropwise add jar, and flowmeter and flow rate control valve are installed in proper order to dropping pipe lower half, and the first half periphery of dropping pipe is personally submitted the symmetry and is provided with two extension pipes rather than being linked together, and two extension pipe tops and dropping pipe top all are provided with the ring flange, and the dropping pipe top is through flange seal installation dropwise add jar, and flange seal lid is installed through flange seal on two extension pipe tops.

Further, the contactless stirring mechanism is including first aeration pipe, the second aeration pipe, the third aeration pipe, the fourth aeration pipe, the intercommunication pipeline, external intercommunication pipeline, first aeration pipe is installed in the inside middle part of the jar of salting out for the vertical form, and second aeration pipe and third aeration pipe are perpendicular form respectively and install in the jar inside part of salting out of first aeration pipe right side and left side, the fourth aeration pipe is installed in the adjacent discharge pipe position in the inside middle of the jar of salting out for the horizontal form, first aeration pipe, the second aeration pipe, be linked together through the intercommunication pipeline between third aeration pipe and the fourth aeration pipe, and intercommunication pipeline one side intercommunication has an external intercommunication pipeline, external air current input device of external intercommunication pipeline.

Compared with the prior art, the invention has the following beneficial effects:

the periphery of the upper half part of the dropping pipe is symmetrically provided with two extension pipes communicated with the extension pipes, and the top ends of the two extension pipes and the top end of the dropping pipe are provided with the flange plates.

The invention relates to an external air flow input device of an external communicating pipeline, which inputs generated air flow into a first aeration pipe, a second aeration pipe, a third aeration pipe and a fourth aeration pipe through the external communicating pipeline and the communicating pipeline, the air flow diffuses and escapes in a bubble form from the micropore parts of the first aeration pipe, the second aeration pipe, the third aeration pipe and the fourth aeration pipe, because the fourth aeration pipe is horizontally arranged at the adjacent discharge pipe part in the middle of the salting-out tank, the air flow diffuses and escapes in a bubble form from the micropore part of the fourth aeration pipe to stir sediment on the bottom surface of the inner cavity of the salting-out tank, and when the sediment stirred by the fourth aeration pipe is lifted along the solution in the salting-out tank, the air flow diffuses and escapes in a bubble form through the micropore parts of the first aeration pipe, the second aeration pipe and the third aeration pipe which are vertically arranged in the salting-out tank to realize convection and uniform mixing, realize contactless stirring, accelerate mixing.

Because the microporous part of the fourth aeration pipe disperses the escaping airflow in the form of bubbles to stir the sediment on the bottom surface of the inner cavity of the salting-out tank, the solution in the stirring state is pumped into the circulating pipeline from the discharge pipe through the positive-pressure pump and then is input into the salting-out tank again from the part communicated with the salting-out tank, the circulation of the solution in the salting-out tank is realized, and the uniform mixing efficiency of the dropwise added solution in the salting-out tank is further improved.

Drawings

FIG. 1 is a schematic sectional view of a salting-out tank of the present invention.

Fig. 2 is a schematic front view of the present invention.

Fig. 3 is a partially enlarged structural view of a portion of the first aeration pipe in fig. 1 according to the present invention.

FIG. 4 is a partially enlarged schematic view of the dropping pipe according to the present invention.

Fig. 5 is a partially enlarged structural view of the cylindrical portion of fig. 1 according to the present invention.

Fig. 6 is a schematic view of the structure of the invention in the direction of a in fig. 5.

In the drawings, the corresponding relationship between the component names and the reference numbers is as follows:

1. salting out a tank; 101. an outer heating layer; 102. a can lid; 103. a pH meter; 104. a temperature meter; 105. a discharge pipe; 106. a cylinder; 107. a disc; 108. a pressure relief through hole; 109. a rubber sheet; 1010. cutting off the port; 2. a circulation pipe; 201. a positive pump; 202. an electromagnetic valve; 3. a dropping pipe; 301. a flow meter; 302. a flow rate control valve; 303. an extension pipe; 304. a flange plate; 305. a flange sealing cover; 306. a dropping tank; 4. a non-contact stirring mechanism; 401. a first aeration pipe; 402. a second aeration pipe; 403. a third aeration pipe; 404. a fourth aeration pipe; 405. a communicating pipe; 406. is externally connected with a communicating pipeline.

Detailed Description

The embodiments of the present invention will be described in further detail with reference to the drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example (b):

as shown in figures 1 to 6:

the invention provides a circulating salting-out dripping device based on collagen extraction, which comprises: the salting-out tank 1 is characterized in that a circulating pipeline 2 communicated with an inner cavity of the salting-out tank 1 is arranged at the bottom of the salting-out tank 1, and a non-contact stirring mechanism 4 is arranged in the salting-out tank 1; the salting-out tank 1 comprises an external heating layer 101 and a tank cover 102, the tank cover 102 is arranged on the top flange of the salting-out tank 1 in a sealing mode, and the external heating layer 101 is arranged on the peripheral surface of the salting-out tank 1; the top of the tank cover 102 is provided with a dropping pipe 3 communicated with the inner cavity of the tank cover.

Wherein, salting-out jar 1 is including PH meter 103, temperature measurement meter 104 and discharge pipe 105, and PH meter 103 and temperature measurement meter 104 are installed respectively to the both ends about cover 102, and PH meter 103 and temperature measurement meter 104 measuring end all are located salting-out jar 1 inner chamber bottom but not rather than the inner chamber face contact, and salting-out jar 1 bottom end face is provided with the discharge pipe 105 rather than the inner chamber is linked together.

The salting-out tank 1 comprises a cylinder 106, a disc 107 and a pressure relief through hole 108, the right end of the tank cover 102 is provided with the cylinder 106, the top end face of the cylinder 106 is provided with the disc 107 which is coaxial with the cylinder 106, the diameter of the disc 107 is larger than that of the cylinder 106, the axle center part of the top end face of the disc 107 is provided with the pressure relief through hole 108 which penetrates through the bottom end face of the cylinder 106, and the pressure relief through hole 108 is communicated with the inner cavity of the tank cover 102.

The salting-out tank 1 comprises a rubber sheet 109 and a cutting opening 1010, the top end face of the disc 107 is provided with the rubber sheet 109 with the diameter consistent with that of the rubber sheet 109, the axis of the rubber sheet 109 is provided with the cutting opening 1010, the cutting opening 1010 is of a cross structure, the size of the cutting opening 1010 is larger than that of the pressure relief through hole 108, and the cutting surface of the cutting opening 1010 is in a sealed closed state under a common state.

Wherein, the circulating pipeline 2 comprises a positive pump 201 and an electromagnetic valve 202, the head end of the circulating pipeline 2 is fixedly connected with the discharge pipe 105, the head end part of the circulating pipeline 2 is provided with the electromagnetic valve 202, the tail end of the circulating pipeline 2 is connected with the top end part of the peripheral surface of the salting-out tank 1, the circulating pipeline 2 is circularly communicated with the inner cavity of the salting-out tank 1, and the circulating pipeline 2 is provided with the positive pump 201.

Wherein, the dropping pipe 3 is including flowmeter 301, flow rate control valve 302, extension pipe 303, ring flange 304, flange seal lid 305 and dropwise add jar 306, flowmeter 301 and flow rate control valve 302 are installed in proper order to dropping pipe 3 lower half, the first half periphery of dropping pipe 3 is the symmetry form and is provided with two extension pipes 303 rather than being linked together, two extension pipe 303 tops and the 3 tops of dropping pipe all are provided with ring flange 304, dropwise add jar 306 is installed through ring flange 304 flange seal on the 3 tops of dropping pipe, flange seal lid 305 is installed through ring flange 304 flange seal on two extension pipe 303 tops.

The non-contact stirring mechanism 4 comprises a first aeration pipe 401, a second aeration pipe 402, a third aeration pipe 403, a fourth aeration pipe 404, a communication pipeline 405 and an external communication pipeline 406, wherein the first aeration pipe 401 is vertically installed in the middle of the salting-out tank 1, the second aeration pipe 402 and the third aeration pipe 403 are respectively vertically installed in the salting-out tank 1 at the right side and the left side of the first aeration pipe 401, the fourth aeration pipe 404 is horizontally installed in the adjacent discharge pipe 105 at the middle of the salting-out tank 1, the first aeration pipe 401, the second aeration pipe 402, the third aeration pipe 403 and the fourth aeration pipe 404 are communicated through the communication pipeline 405, one side of the communication pipeline 405 is communicated with the external communication pipeline 406, and the external communication pipeline 406 is externally connected with an airflow input device.

When in use:

the periphery of the upper half part of the dropping pipe 3 is symmetrically provided with two extension pipes 303 communicated with the same, the top ends of the two extension pipes 303 and the top end of the dropping pipe 3 are both provided with flange plates 304, three groups of dropping tanks 306 can be simultaneously and fixedly arranged through the arrangement of the dropping pipe 3 and the two extension pipes 303 communicated with the same, the simultaneous dropping of three different substances can be realized, the top ends of the two extension pipes 303 are provided with flange sealing covers 305 in a flange sealing way through the flange plates 304, and the arrangement of the flange sealing covers 305 plays a role in sealing the opening ends of the two extension pipes 303 when the two extension pipes are not applied;

when the salting-out tank is used, the external communication pipeline 406 is externally connected with an airflow input device, the airflow input device inputs generated airflow into the first aeration pipe 401, the second aeration pipe 402, the third aeration pipe 403 and the fourth aeration pipe 404 through the external communication pipeline 406 and the communication pipeline 405, the airflow diffuses and escapes in a bubble form from the micropore parts of the first aeration pipe 401, the second aeration pipe 402, the third aeration pipe 403 and the fourth aeration pipe 404, because the fourth aeration pipe 404 is horizontally arranged in the middle of the salting-out tank 1 and is adjacent to the discharge pipe 105, the airflow diffuses and escapes in a bubble form from the micropore part of the fourth aeration pipe 404 to stir sediment on the bottom surface of the inner cavity of the salting-out tank 1, and when the sediment stirred by the fourth aeration pipe 404 is lifted along the solution in the salting-out tank 1, the airflow diffusing and uniformly mixing is realized in a bubble form through the micropore parts of the first aeration pipe 401, the second aeration pipe 402 and the third aeration pipe 403 which are vertically arranged in the salting-out tank 1, non-contact stirring is realized, and uniform mixing is accelerated; furthermore, the axle center part of the top end surface of the disc 107 is provided with a pressure relief through hole 108 which runs through the bottom end surface of the cylinder 106, and the pressure relief through hole 108 is communicated with the inner cavity of the tank cover 102, so that the air flow which is input into the inner cavity of the salting tank 1 through the first aeration pipe 401, the second aeration pipe 402, the third aeration pipe 403 and the fourth aeration pipe 404 expands the cutting opening 1010 which is in a sealed and closed state in daily life, and is discharged from the pressure relief through hole 108, thereby ensuring that the air pressure in the salting tank 1 is kept at a normal level;

because the microporous part of the fourth aeration pipe 404 disperses the escaping airflow in the form of bubbles to stir the sediment on the bottom surface of the inner cavity of the salting-out tank 1, the solution in the stirring state is pumped into the circulating pipeline 2 from the discharge pipe 105 through the positive-pressure pump 201, and then is input into the salting-out tank 1 again from the part communicated with the salting-out tank 1, the circulation of the solution in the salting-out tank 1 is realized, and the uniform mixing efficiency of the dropwise added solution in the salting-out tank 1 is further improved.

The embodiments of the present invention have been presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the invention in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiment was chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated.