阅读说明：本技术 一种高总酚含量厚朴加工工艺 (Processing technology of mangnolia officinalis with high total phenol content ) 是由 李永凤 于 2021-08-25 设计创作，主要内容包括：本发明公开了一种高总酚含量厚朴加工工艺,所述制备方法包括以下步骤：(1)将厚朴皮干燥、粉碎,得到粗粉；(2)将粗粉溶于蒸馏水中,加入纤维素酶酶解,然后加热进行提取,将超声提取液浓缩,得浸膏；(3)将浸膏用乙醇水溶液溶解,采用大孔树脂凝胶柱纯化,用洗脱液洗脱,得到洗脱液；(4)将洗脱液经过离心去渣,浓缩上清液,冷冻干燥,得到纯化后的厚朴总酚；本发明采用纤维素酶酶解,超声提取,大孔树脂纯化,所使用的溶剂为乙醇水,上述操作对人体及环境均无毒,安全,且成本低,经发明人通过系列试验和系统研究,采用本发明提取方法提制的高纯度厚朴提取物的提取率明显高于现有技术,折合成本最低。(The invention discloses a processing technology of mangnolia officinalis with high total phenol content, which comprises the following steps: (1) drying and crushing cortex magnoliae officinalis to obtain coarse powder; (2) dissolving the coarse powder in distilled water, adding cellulase for enzymolysis, heating for extraction, and concentrating the ultrasonic extract to obtain extract; (3) dissolving the extract with ethanol water solution, purifying with macroporous resin gel column, and eluting with eluent to obtain eluent; (4) centrifuging the eluate to remove residue, concentrating the supernatant, and freeze drying to obtain purified total phenols of cortex Magnolia officinalis; the method adopts cellulase enzymolysis, ultrasonic extraction and macroporous resin purification, the used solvent is ethanol water, the operation is nontoxic and safe to human bodies and environment, and the cost is low.)

1. The processing technology of the magnolia officinalis with high total phenol content is characterized in that the preparation method comprises the following steps:

(1) drying the magnolia bark, and then crushing and sieving the dried magnolia bark with a 60-80 mesh sieve;

(2) dissolving the coarse powder in distilled water, adding cellulase for enzymolysis, heating for ultrasonic extraction, and concentrating the extract to obtain extract;

(3) dissolving the extract with ethanol water solution, purifying with macroporous resin gel column, eluting with eluent, and collecting eluent;

(4) and centrifuging the eluent to remove residues, concentrating the supernatant, and freeze-drying to obtain the purified magnolia officinalis total phenol.

2. The method according to claim 1, wherein in the step (2), the cellulase is present in the distilled water in an amount of 0.1 to 0.5% by mass.

3. The method according to claim 2, wherein the cellulase is contained in distilled water at a mass fraction of 0.2%.

4. The preparation method according to claim 1, wherein in the step (2), the feed-liquid ratio of the coarse powder to the distilled water is 1: (10-20).

5. The preparation method according to claim 1, wherein in the step (2), the enzymolysis time is 30-60min, and the enzymolysis temperature is 20-40 ℃.

6. The preparation method according to claim 1, wherein in the step (2), the ultrasonic extraction temperature is 20-60 ℃, the extraction time is 30-60min, and the ultrasonic frequency is 3 times.

7. The method according to claim 1, wherein in the step (3), the volume fraction of ethanol in the ethanol aqueous solution is 5% to 20%, and the eluent is 20% to 50% of the ethanol aqueous solution.

8. The method according to claim 1, wherein in the step (3), the ratio of the volume of the extract dissolved in the aqueous ethanol solution to the volume of the gel column is 1: (10-40), the ratio of the column diameter to the column height of the macroporous resin gel column is 1 (50-70), and the sample loading flow rate is 1-5 mL/min when the macroporous resin gel column is used for purification.

9. The magnolia total phenol prepared by the preparation method of any one of claims 1 to 8.

Technical Field

The invention relates to a processing technology of magnolia officinalis with high total phenol content.

Background

The magnolol is prepared from dried bark or branch bark of perennial Magnolia bark (Magnolia officinalis of Magnoliaceae, et Wils.) or Magnolia officinalis of Orthosiphon (Magnolia officinalis of Magnolia officinalis Rehd, et Wils. var. bioba Rehd, et Wils.) of plants by extracting, extracting and refining to obtain high content of total magnolol extract. The main effective components of the magnolia officinalis are magnolol, honokiol, iso-magnolol, tetrahydromagnolol, magnoline and the like, wherein the content of the magnolol and the honokiol is the highest, the content of the two phenols in the magnolia officinalis is more than 2% specified in Chinese pharmacopoeia, the magnolia officinalis can be used for medicine, and the content of the trunk bark of a tree growing for about 15 years is 3-5% generally and is higher.

Magnolol and honokiol in cortex Magnolia officinalis have obvious and lasting central muscle relaxation, central nerve inhibiting effect, antiinflammatory, antibacterial, anti-pathogenic microorganism, antiulcer, antioxidant, anti-tumor, morphine withdrawal reaction inhibiting, platelet aggregation inhibiting, etc., and can be used for treating mental diseases.

In order to fully exert the pharmacological and medicinal effects of magnolia officinalis, the current research reports on magnolol and honokiol include the following extraction methods: alkali extraction and acid precipitation, water extraction, ethanol extraction, alkali extraction, supercritical CO2 extraction, high-speed countercurrent extraction, silica gel column chromatography, macroporous resin adsorption, TLC preparative separation, HPLC preparation, etc. The used medicinal materials are all magnolia officinalis raw materials processed by a traditional method, including magnolia officinalis, magnolia officinalis and the like, and wild resources are often damaged in order to reduce the production cost and even require the magnolia officinalis with the total phenol content of 8 percent for more than 20 years. Although the ethanol extraction method can obtain higher extraction rate in the initial extraction, the content of the obtained product is not high, and the subsequent treatment of the high-purity product is difficult; the supercritical CO2 extraction method and equipment are expensive, the early investment is large, the cost is too high, and large-scale industrial production is not easy to realize; although the silica gel column chromatography has the characteristics of good separation effect on effective components and high product yield, large-scale industrial production is difficult to realize; the macroporous resin method can realize industrial production, but has longer period and higher cost. TLC and HPLC methods are only suitable for laboratory miniprep and detection and cannot be used for large-scale industrial production applications; when petroleum ether and cyclohexane are adopted for reflux extraction of crude products obtained by an alkali extraction method and an alkali extraction acid precipitation method, the extraction rate of effective components is not high, the solvent consumption is large, and great potential safety hazards exist due to repeated transfer of a large amount of solvents.

Disclosure of Invention

The invention provides a processing technology of magnolia officinalis with high total phenol content aiming at the defects of the prior art.

The invention achieves the above object through the following technical scheme.

A processing technology of mangnolia officinalis with high total phenol content comprises the following steps:

(1) drying the magnolia bark, and then crushing and sieving the dried magnolia bark with a 60-80 mesh sieve;

(2) dissolving the coarse powder in distilled water, adding cellulase for enzymolysis, heating for ultrasonic extraction, and concentrating the extract to obtain extract;

(3) dissolving the extract with ethanol water solution, purifying with macroporous resin gel column, eluting with eluent, and collecting eluent;

(4) and centrifuging the eluent to remove residues, concentrating the supernatant, and freeze-drying to obtain the purified magnolia officinalis total phenol.

Preferably, in the step (2), the mass fraction of the cellulase in the distilled water is 0.1-0.5%.

Preferably, the mass fraction of cellulase in distilled water is 0.2%.

Preferably, in the step (2), the feed-liquid ratio of the coarse powder to the distilled water is 1: (10-20).

Preferably, in the step (2), the enzymolysis time is 30-60min, and the enzymolysis temperature is 20-40 ℃.

Preferably, in the step (2), the ultrasonic extraction temperature is 20-60 ℃, the extraction time is 30-60min, and the ultrasonic frequency is 3 times.

Preferably, in the step (3), the volume fraction of ethanol in the ethanol aqueous solution is 5-20%, and the eluent is 20-50% of the ethanol aqueous solution.

Preferably, in the step (3), the volume ratio of the volume of the extract dissolved in the ethanol water solution to the volume of the gel column is 1: (10-40), the ratio of the column diameter to the column height of the macroporous resin gel column is 1 (50-70), and the sample loading flow rate is 1-5 mL/min when the macroporous resin gel column is used for purification.

Has the advantages that:

the magnolia bark processing technology with high total phenol content adopts cellulose enzyme hydrolysis, ultrasonic extraction and macroporous resin purification, uses ethanol water as a solvent, and has the advantages of no toxicity to human bodies and environment, safety and low cost. Through serial tests and systematic research, the extraction rate of the high-purity magnolia officinalis extract extracted by the extraction method is obviously higher than that of the prior art, and the cost is the lowest.

Detailed Description

The present invention will be further described with reference to the following examples, which are intended to illustrate the present invention and are not intended to limit the present invention.

Example 1

A processing technology of mangnolia officinalis with high total phenol content comprises the following steps:

(1) drying the magnolia bark, and then crushing and sieving the dried magnolia bark with a 60-mesh sieve;

(2) dissolving the coarse powder in distilled water, adding cellulase for enzymolysis, heating for ultrasonic extraction, and concentrating the extract to obtain extract;

(3) dissolving the extract with ethanol water solution, purifying with macroporous resin gel column, eluting with eluent, and collecting eluent;

(4) and centrifuging the eluent to remove residues, concentrating the supernatant, and freeze-drying to obtain the purified magnolia officinalis total phenol.

Specifically, in the step (2), the mass fraction of the cellulase in the distilled water is 0.1%.

Specifically, in the step (2), the ratio of the coarse powder to the distilled water is 1: 10.

specifically, in the step (2), the enzymolysis time is 30min, and the enzymolysis temperature is 20 ℃.

Specifically, in the step (2), the ultrasonic extraction temperature is 20 ℃, the extraction time is 30min, and the ultrasonic frequency is 3 times.

Specifically, in the step (3), the volume fraction of ethanol in the ethanol aqueous solution is 5%, and the eluent is 20% of the ethanol aqueous solution.

Specifically, in the step (3), the volume ratio of the volume of the extract dissolved in the ethanol water to the volume of the gel column is 1: 10, the ratio of the column diameter to the column height of the macroporous resin gel column is 1:50, and the sample loading flow rate is 1mL/min when the macroporous resin gel column is used for purification.

Example 2

A processing technology of mangnolia officinalis with high total phenol content comprises the following steps:

(1) drying the magnolia bark, and then crushing and sieving the magnolia bark by a 80-mesh sieve;

(2) dissolving the coarse powder in distilled water, adding cellulase for enzymolysis, heating for ultrasonic extraction, and concentrating the extract to obtain extract;

(3) dissolving the extract with ethanol water solution, purifying with macroporous resin gel column, eluting with eluent, and collecting eluent;

(4) and centrifuging the eluent to remove residues, concentrating the supernatant, and freeze-drying to obtain the purified magnolia officinalis total phenol.

Specifically, in the step (2), the mass fraction of the cellulase in the distilled water is 0.5%.

Specifically, in the step (2), the ratio of the coarse powder to the distilled water is 1: 20.

specifically, in the step (2), the enzymolysis time is 60min, and the enzymolysis temperature is 40 ℃.

Specifically, in the step (2), the ultrasonic extraction temperature is 60 ℃, the extraction time is 60min, and the ultrasonic frequency is 3 times.

Specifically, in the step (3), the volume fraction of ethanol in the ethanol aqueous solution is 20%, and the eluent is a 50% ethanol aqueous solution.

Specifically, in the step (3), the volume ratio of the volume of the extract dissolved in the ethanol water to the volume of the gel column is 1: 40, the ratio of the column diameter to the column height of the macroporous resin gel column is 1:70, and the sample loading flow rate is 5mL/min when the macroporous resin gel column is used for purification.

Example 3

A processing technology of mangnolia officinalis with high total phenol content comprises the following steps:

(1) drying the magnolia bark, and then crushing and sieving the dried magnolia bark with a 60-80 mesh sieve;

(2) dissolving the coarse powder in distilled water, adding cellulase for enzymolysis, heating for ultrasonic extraction, and concentrating the extract to obtain extract;

(3) dissolving the extract with ethanol water solution, purifying with macroporous resin gel column, eluting with eluent, and collecting eluent;

(4) and centrifuging the eluent to remove residues, concentrating the supernatant, and freeze-drying to obtain the purified magnolia officinalis total phenol.

Specifically, in the step (2), the mass fraction of the cellulase in the distilled water is 0.2%.

Specifically, in the step (2), the ratio of the coarse powder to the distilled water is 1: 15.

specifically, in the step (2), the enzymolysis time is 40min, and the enzymolysis temperature is 35 ℃.

Specifically, in the step (2), the ultrasonic extraction temperature is 40 ℃, the extraction time is 40min, and the ultrasonic frequency is 3 times.

Specifically, in the step (3), the volume fraction of ethanol in the ethanol aqueous solution is 10%, and the eluent is an ethanol aqueous solution with a volume fraction of 30%.

Specifically, in the step (3), the volume ratio of the volume of the extract dissolved in the ethanol water to the volume of the gel column is 1: 25, the ratio of the column diameter to the column height of the macroporous resin gel column is 1:60, and the sample loading flow rate is 3mL/min when the macroporous resin gel column is used for purification.

Detection result (Magnolia officinalis total phenol ═ magnolol + honokiol)

Note: the magnolol and honokiol determination method adopts high performance liquid chromatography of 234 pages of the second part of 2010 Chinese pharmacopoeia. The extraction yield is (α × M- β × M)/M × 100%; wherein, α and β: the content of total phenols in the magnolia officinalis sample; m: the quality of the extracted residue; m: the mass of the sample.