阅读说明：本技术 用于改善皮肤透明度及暗沉的组合物 (Composition for improving skin transparency and darkness ) 是由 赵嘉英 权里耕 崔炤雄 朴录贤 李垠秀 徐丙徽 赵诚雅 于 2019-08-26 设计创作，主要内容包括：本说明书涉及一种用于改善皮肤透明度的组合物及用于改善皮肤暗沉的组合物。所述组合物包含作为有效成分的野蔷薇果、黑芝麻、梅果、榅桲及松子的混合提取物,其中所述野蔷薇果及黑芝麻可为发芽的。此外,所述组合物对随着年龄增加、或者因外部环境或压力等影响而变得暗沉的皮肤具有改善效果,使皮肤变得干净和透明。(The present specification relates to a composition for improving skin transparency and a composition for improving skin dullness. The composition comprises mixed extracts of fructus Rosae Davuricae, semen Sesami Niger, mume fructus, fructus Cydoniae Oblongae and semen Pini as effective components, wherein the fructus Rosae Davuricae and semen Sesami Niger can be germinated. In addition, the composition has an improving effect on skin which becomes dull with age or due to the influence of external environment or pressure, etc., to make the skin clean and transparent.)

1. A composition for improving skin transparency comprises mixed extracts of fructus Rosae Davuricae, semen Sesami Niger, mume fructus, fructus Cydoniae Oblongae and semen Pini as effective components.

2. A composition for improving skin darkness comprises mixed extracts of fructus Rosae Davuricae, semen Sesami Niger, mume fructus, fructus Cydoniae Oblongae and semen Pini as effective components.

3. The composition of claim 1 or 2, wherein the fructus Rosae Davuricae and semen Sesami Niger are germinated fructus Rosae Davuricae and germinated semen Sesami Niger.

4. The composition of claim 3, wherein the germinated fructus Rosae Davuricae, germinated semen Sesami Niger, mume fructus, quince and semen Pini are mixed and extracted at a weight ratio of 10-30: 20-40: 10-25: 15-30.

5. The composition as claimed in claim 1 or 2, wherein the extract is an extract of one or more solvents selected from the group consisting of water and lower alcohols having 1 to 4 carbon atoms.

6. The composition of claim 5, wherein the extract is an ethanol extract.

7. The composition of claim 1 or 2, wherein the composition comprises from 0.001% to 20% by weight of the mixed extract.

8. Composition according to claim 1 or 2, characterized in that it is a cosmetic composition.

9. The composition according to claim 1 or 2, wherein the composition is in the form of a lotion, an emulsion, an essence, a cream, a gel or a mask.

10. The composition of claim 1, wherein the skin transparency is the ratio of transmitted light to incident light at the skin surface.

11. The composition according to claim 1, wherein when the diffuse reflectance value of the inside of the skin before and after the use of the composition is measured at the skin surface at angles of an incident angle of 60 ° and a reflection angle of 60 °, the rate of increase of the diffuse reflectance value of the inside of the skin after the use of the composition relative to that before the use is 2% or more.

12. The composition according to claim 2, wherein the composition is used for suppressing generation of a substance exhibiting autofluorescence under the conditions of fluorescence measurement of excitation light at 340nm and emission light at 440 nm.

Technical Field

The present specification relates to a composition for improving skin transparency and a composition for improving skin dullness.

Background

The existing whitening cosmetics have been developed to whiten the skin by simply removing abnormally expressed melanin, but in recent years, there is an increasing demand for products having increased effects such as transparent skin color and transparent feeling, on top of simply whitening the skin. However, the existing whitening raw materials having a melanin synthesis inhibiting effect cannot sufficiently satisfy such needs of customers.

In recent years, related studies are actively being conducted on factors affecting skin color or skin transparency other than melanin. It is studied that skin transparency is determined by several factors regulating light scattering and refraction in actual skin in combination, for example, melanin, skin uniformity, moisture content, hemoglobin, etc. are considered as main influencing factors, in addition to simple skin pigments. Due to the combined action of these several factors, skin transparency varies depending on the amount of light transmitted by the skin itself.

[ Prior art documents ]

[ patent document ]

Patent document 1: korean patent laid-open No. 10-2015-0057667A;

patent document 2: japanese patent laid-open No. 2004-509912A.

Disclosure of Invention

Technical problem

In one aspect, the present specification aims to provide a composition for improving skin transparency, which comprises a mixed extract of dog rose fruit, black sesame, plum fruit, quince and pine nut as an effective ingredient.

In another aspect, the present specification aims to provide a composition for improving skin dullness, which comprises a mixed extract of dog rose fruit, black sesame, plum fruit, quince and pine nut as an effective ingredient.

Technical scheme

In one aspect, the technology disclosed in the present specification provides a composition for improving skin transparency, the composition comprising, as effective ingredients, mixed extracts of dog rose fruit, black sesame, plum fruit, quince and pine nut.

In another aspect, the technology disclosed in the present specification provides a composition for improving skin dullness, the composition comprising a mixed extract of dog rose fruit, black sesame, plum fruit, quince and pine nut as an effective ingredient.

In an exemplary embodiment, the dog rose fruit and black sesame may be germinated dog rose fruit and germinated black sesame.

In an exemplary embodiment, the germinated dog rose fruit, germinated black sesame, plum fruit, quince and pine nut may be mixed and extracted in a weight ratio of 10 to 30:20 to 40:10 to 25:15 to 30.

In an exemplary embodiment, the extract may be an extract of at least one solvent selected from the group consisting of water and lower alcohols having 1 to 4 carbon atoms.

In an exemplary embodiment, the extract may be an ethanol extract.

In an exemplary embodiment, the composition may include 0.001 wt% to 20 wt% of the mixed extract.

In one exemplary embodiment, the composition may be a cosmetic composition.

In an exemplary embodiment, the composition may be prepared in the form of a lotion, an emulsion, an essence, a cream, a gel, or a mask.

In an exemplary embodiment, the skin transparency can be a ratio of transmitted light to incident light on the skin surface.

In an exemplary embodiment, when the internal diffuse reflection (surface reflection) values of the skin before and after using the composition are measured at the surface of the skin at angles of an incident angle of 60 ° and a reflection angle of 60 °, the increase rate of the internal diffuse reflection value of the skin after using the composition with respect to that before using may be 2% or more.

In an exemplary embodiment, the composition can inhibit the generation of a substance exhibiting autofluorescence (autofluorescence) under fluorometric conditions with excitation light (excitation) at 340nm and emission light (emission) at 440 nm.

Advantageous effects

In one aspect, the technology disclosed in the present specification has the effect of providing a composition for improving skin transparency and dullness, which comprises a mixed extract of dog rose fruit, black sesame, plum fruit, quince and pine nut as an effective ingredient.

The composition disclosed in the present specification has an improving effect on skin that becomes dull with age or due to the influence of external environment or pressure, etc., and makes the skin clean and transparent.

Drawings

Fig. 1 shows a flow diagram for the preparation of a mixed extract according to one embodiment of the present description.

Fig. 2 shows the result of cytotoxicity evaluation of the mixed extract according to one embodiment of the present specification.

Fig. 3 shows the results of evaluation of the skin dullness improving efficacy of the mixed extracts according to one embodiment of the present specification (, p < 0.01;, p <0.001vs. ribose-treated group).

FIG. 4 shows the results of an evaluation of the efficacy of the mixed extracts for improving skin clarity (mean. + -. SD;, p < 0.05;, p <0.001vs. before use) according to one embodiment of the present description

Detailed Description

Hereinafter, the present invention will be described in detail.

In one aspect, the technology disclosed in the present specification provides a composition for improving skin transparency, comprising a mixed extract of dog rose fruit, black sesame, plum fruit, quince and pine nut as an effective ingredient.

In another aspect, the technology disclosed in the present specification provides a method for improving skin transparency, which comprises administering to a subject in need thereof a mixed extract of dog rose fruit, black sesame, plum fruit, quince and pine nut in an amount effective to improve skin transparency.

In another aspect, the technology disclosed in the present specification provides a mixed extract of dog rose fruit, black sesame, plum fruit, quince and pine nut for improving skin transparency.

In another aspect, the technology disclosed herein provides a non-therapeutic use of a mixed extract of dog rose fruit, black sesame, plum fruit, quince and pine nut for improving skin transparency.

In another aspect, the technology disclosed in the present specification provides the use of a mixed extract of dog rose fruit, black sesame, plum fruit, quince and pine nut in the preparation of a composition for improving skin transparency.

In yet another aspect, the technology disclosed in the present specification provides a composition for improving skin dullness, comprising a mixed extract of dog rose fruit, black sesame, plum fruit, quince and pine nut as an effective ingredient.

In another aspect, the technology disclosed in the present specification provides a method for improving skin dullness, comprising administering to a subject in need thereof a mixed extract of dog rose fruit, black sesame, plum fruit, quince and pine nut in an amount effective to improve skin dullness.

In another aspect, the technology disclosed in the present specification provides a mixed extract of dog rose fruit, black sesame, plum fruit, quince and pine nut for improving skin dullness.

In another aspect, the technology disclosed herein provides a non-therapeutic use of a mixed extract of dog rose fruit, black sesame, plum fruit, quince and pine nut for improving skin dullness.

In another aspect, the technology disclosed in the present specification provides the use of a mixed extract of dog rose fruit, black sesame, plum fruit, quince and pine nut for the preparation of a composition for improving skin dullness.

In an exemplary embodiment, the mixed extract of dog rose fruit, black sesame, plum fruit, quince fruit and pine nut or the composition may be administered to the subject in one or more forms selected from a pharmaceutical composition, a skin external composition, a food composition or a cosmetic composition.

In an exemplary embodiment, the mixed extract of dog rose fruit, black sesame, plum fruit, quince and pine nut or the composition may be applied to the skin of the subject.

In an exemplary embodiment, the administration can be a dosing or coating to the subject.

In the present specification, the effective ingredient means an ingredient that can exhibit a desired activity alone or together with a carrier or the like that itself is inactive.

In this specification, skin transparency refers to the ratio of transmitted light to incident light on the skin surface. A part of the incident light is scattered and scattered at the skin surface, the remaining part is transmitted to the inside of the skin, and the transmitted light (transmitted light) is reflected at the inside of the skin and then emitted again. Skin transparency increases with the amount of light reflected from the inside of the skin (diffuse reflection).

In an exemplary embodiment, the skin transparency is a ratio of transmitted light on the skin surface, which can be measured by a diffuse reflection (surface reflection) value inside the skin reflected from the inside of the skin, to incident light.

In an exemplary embodiment, when the internal diffuse reflectance (surface reflectance) values of the skin before and after using the mixed extract or the composition are measured at the surface of the skin at an incident angle of 60 ° and a reflection angle of 60 °, the rate of increase of the internal diffuse reflectance value of the skin after using the mixed extract or the composition relative to that before using may be 2% or more, 3% or more, 4% or more, 5% or more, or 6% or more.

In another exemplary embodiment, the rate of increase of the internal diffuse reflectance of the skin after using the mixed extract or the composition relative to the internal diffuse reflectance of the skin before use may be 2% to 12%, 2% to 10%, or 2% to 8%, when the internal diffuse reflectance of the skin before and after using the mixed extract or the composition is measured at the surface of the skin at an angle of an incident angle of 60 ° and a reflection angle of 60 °.

In an exemplary embodiment, the diffuse reflectance value inside the scattering skin may use Radioscan^TM(True system, Korea).

In the present specification, skin darkness may refer to the following states: darkening of skin tone due to degradation of the lightness and chroma of the skin, or unevenness of skin tone due to local appearance of dark and dark tint. Dullness of the skin is caused by various complicated factors such as physiological factors, genetic factors, environmental factors, and the like.

In an exemplary embodiment, the mixed extract or the composition may inhibit generation of or reduce a substance exhibiting autofluorescence (autofluorescence) under fluorescence measurement conditions of excitation light (excitation) at 340nm and emission light (emission) at 440nm when applied to skin.

The mixed extract or the composition comprising the same according to the present specification has the effects of cleansing dark (dull) skin and improving skin transparency.

In an exemplary embodiment, the use of the mixed extract or the composition may be the application of the mixed extract or the composition to the skin for 2 weeks or more, 4 weeks or more, or 4 weeks to 8 weeks.

In an exemplary embodiment, the use of the mixed extract or the composition may be to apply the mixed extract or composition to the skin twice a day.

In the present specification, the mixed extract may refer to a mixture of extracts extracted from one kind of extraction raw material, a mixture of extracts obtained by mixing and extracting one or more kinds of extraction raw materials, or an extract obtained by mixing and extracting five kinds of extraction raw materials. The extraction raw materials include fructus Rosae Davuricae, semen Sesami Niger, mume fructus, fructus Cydoniae Oblongae and semen Pini.

In an exemplary embodiment, the dog rose fruit and black sesame may be germinated dog rose fruit and germinated black sesame.

The fructus Rosae Davuricae refers to fruit of Rosa multiflora Thunberg (Rosa multiflora Thunberg) of Rosaceae, which usually grows in mountain.

In an exemplary embodiment, the germinated fructus rosae multiflorae, the germinated black sesame, the plum fruit, the quince and the pine nut are preferably mixed and extracted in a weight ratio of 10 to 30:20 to 40:10 to 25:15 to 30, from the viewpoint of the efficacy of improving skin transparency and improving skin dullness.

In an exemplary embodiment, the extract refers not only to a crude extract, but also to a processed product of the crude extract, including, for example, all forms obtained by additional processing such as drying, concentration, fractionation, purification, fermentation, suspension, and the like, and the additional processing may include one or more processes. For example, the fractionated extract includes a fraction obtained by suspending the crude extract in a certain solvent, mixing with a solvent having a different polarity, and standing, and a fraction obtained by fractionating the crude extract with successive solvents. In addition, the extract of the present specification also includes fractions obtained by various purification methods additionally performed as follows: such as separation using ultrafiltration membranes having a certain molecular weight cut-off, separation by various chromatographic methods (separation based on size, charge, hydrophobicity or amphiphilicity).

In an exemplary embodiment, the method of preparing the extract may be any means according to conventional methods, considering the extraction degree or preservation degree of the effective ingredients, for example, hot water extraction, immersion extraction, steam distillation, cold extraction, reflux cooling extraction, ultrasonic extraction, supercritical extraction, subcritical extraction, solvent extraction, elution, compression, high temperature extraction, high pressure extraction, extraction using an adsorption resin comprising XAD and HP-20, or fermentation or natural fermentation metabolism using microorganisms. The number of extraction times may be 1 to 5 times, and a method such as distillation under reduced pressure, concentration under reduced pressure, freeze-drying or spray-drying may be additionally performed after the extraction.

In an exemplary embodiment, the extract may be extracted with one or more solvents selected from the group consisting of water, anhydrous or hydrated lower alcohols having 1 to 4 carbon atoms (e.g., methanol, ethanol, propanol, butanol, etc.), ethylene, acetone, hexane, diethyl ether, chloroform, ethyl acetate, butyl acetate, methylene chloride, N-Dimethylformamide (DMF), methylene chloride, dimethyl sulfoxide (DMSO), glycerol, butylene glycol, propylene glycol, dipropylene glycol (dipropylene glycol), methylene chloride, diethyl ether, and mixtures thereof.

In an exemplary embodiment, the extract may be an extract of one or more solvents selected from the group consisting of water and lower alcohols having 1 to 4 carbon atoms.

In an exemplary embodiment, the extract may be an ethanol extract. The ethanol extract is extracted by using an extraction solvent consisting of only ethanol or an extraction solvent in which water and ethanol are mixed.

In an exemplary embodiment, the extract may be prepared by: soaking fructus Rosae Davuricae and semen Sesami Niger in water for germination; and mixing germinated fructus Rosae Davuricae and germinated semen Sesami Niger with mume fructus, fructus Cydoniae Oblongae and semen Pini, and extracting.

In an exemplary embodiment, the impregnation may be performed for 2 hours to 20 hours.

In an exemplary embodiment, the germination may be performed at room temperature, e.g., 15 ℃ to 25 ℃ or 20 ℃ to 25 ℃.

In an exemplary embodiment, the germination may be performed for 30 hours to 60 hours.

In an exemplary embodiment, the extract may be extracted by an ethanol solvent at a concentration of 30% (v/v) to 80% (v/v). In another exemplary embodiment, the extract may be extracted by an ethanol solvent at a concentration (v/v) of 30% or more, 35% or more, 40% or more, 45% or more, 50% or more, 55% or more, 60% or more, 65% or more, 70% or more, or 75% or more, and 80% or less, 75% or less, 70% or less, 65% or less, 60% or less, 55% or less, 50% or less, 45% or less, 40% or less, or 35% or less.

In an exemplary embodiment, the extract may be extracted at 15 ℃ to 85 ℃. In another exemplary embodiment, the extract can be extracted at a temperature of 15 ℃ or more, 25 ℃ or more, 35 ℃ or more, 45 ℃ or more, 55 ℃ or more, 65 ℃ or more, or 75 ℃ or more, and 85 ℃ or less, 75 ℃ or less, 65 ℃ or less, 55 ℃ or less, 45 ℃ or less, 35 ℃ or less, or 25 ℃ or less.

In an exemplary embodiment, the extraction time may be 1 hour or more, or 1 hour to 24 hours.

In an exemplary embodiment, the composition may include 0.001 wt% to 20 wt% of the mixed extract. In another exemplary embodiment, the composition may provide an excellent effect of improving skin transparency and dullness by including 0.001 wt% or more, 0.005 wt% or more, 0.01 wt% or more, 0.05 wt% or more, 0.1 wt% or more, 0.5 wt% or more, 1 wt% or more, 1.5 wt% or more, 2 wt% or more, 2.5 wt% or more, 3 wt% or more, 3.5 wt% or more, 4 wt% or more, 4.5 wt% or more, or 5 wt% or more, and 20 wt% or less, 15 wt% or less, 10 wt% or less, 5 wt% or less, or 1 wt% or less of the mixed extract.

In an exemplary embodiment, the mixed extract may be included in the composition at a concentration of 10ppm, 15ppm, 20ppm, 25ppm, 30ppm, 35ppm, 40ppm, 45ppm, or 50ppm or more. The concentration of the mixed extract contained in the composition may preferably be 25ppm, 30ppm, 35ppm, 40ppm, 45ppm, or 50ppm or more.

In one exemplary embodiment, the composition may be a pharmaceutical composition.

In an exemplary embodiment, the dosage form of the pharmaceutical composition may be a solution, a suspension, an emulsion, a gel, drops, a suppository, a cream, an ointment, a patch, a pad, or a spray, but is not limited thereto. The dosage form can be easily prepared according to a conventional method in the art, and may further use excipients, wettable powders, emulsifiers, suspending agents, salts or buffers for adjusting osmotic pressure, colorants, flavors, stabilizers, preservatives or other commercial auxiliaries.

Further, the pharmaceutical composition may be orally administered, parenterally administered, rectally administered, topically administered, transdermally administered, intravenously administered, intramuscularly administered, intraperitoneally administered, subcutaneously administered, etc. according to a desired method, and an effective dose of the pharmaceutical composition will vary according to the age, sex, body weight, pathological state and severity thereof of a subject to be administered, administration route or the judgment of a prescriber. It is within the level of the skilled person to determine the appropriate dosage based on these factors, and the dosage administered in one day may be, for example, 0.001 mg/kg/day to 100 mg/kg/day, more specifically, 0.5 mg/kg/day to 50 mg/kg/day, but is not limited thereto.

In one exemplary embodiment, the composition may be a composition for external skin preparation, and the external skin preparation is a generic term that may include any substance applied to the outside of the skin, which may include various dosage forms of pharmaceuticals. For example, the composition may be in the form of an ointment, lotion, gel, cream, spray, suspension, emulsion, patch, etc., but is not limited thereto.

In an exemplary embodiment, the skin external agent composition may further comprise a preservative, a stabilizer, a wettable powder or an emulsifier, a pharmaceutical adjuvant such as a salt and/or a buffer for adjusting osmotic pressure, and other therapeutically useful substances, in addition to the active ingredient according to the present specification.

In one exemplary embodiment, the composition may be a food composition.

In the present specification, the food composition may provide various forms of food additives or functional foods. Specifically, the composition may be processed into extract tea, liquid tea, beverage, fermented milk, cheese, yogurt, fruit juice, probiotic bacteria, health food, or the like, and may be used in the form of various food additives.

In addition, the food composition may further contain other ingredients which produce a synergistic effect on the main effect, within a range not impairing the main effect of the desired active ingredient. For example, additives such as perfumes, pigments, bactericides, antioxidants, preservatives, humectants, thickeners, inorganic salts, emulsifiers, or synthetic high molecular substances may be further included to improve physical properties. In addition, it may further comprise water-soluble vitamins, oil-soluble vitamins, high molecular peptide, high molecular polysaccharide or seaweed extract as auxiliary components. The above-mentioned ingredients can be appropriately selected and formulated by those skilled in the art according to the dosage form or the purpose of use without difficulty, and the addition amount thereof can be selected within a range not impairing the purpose and effect of the present specification. For example, the above ingredients may be added in an amount of 0.001 wt% to 5 wt%, or 0.01 wt% to 3 wt%, based on the total weight of the composition.

In one exemplary embodiment, the composition may be a cosmetic composition.

The cosmetic composition may further include a functional additive and ingredients contained in general cosmetic compositions, in addition to the mixed extract. The functional additive may comprise a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high molecular peptides, high molecular polysaccharides, sphingolipids and seaweed extracts. In addition, the formulation components may be oil and fat components, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, perfumes, blood circulation promoters, coolants, antiperspirants, purified water, etc.

The formulation of the cosmetic composition is not particularly limited, and may be appropriately selected according to the purpose. For example, one or more selected from the group consisting of a moisturizing lotion, a softening lotion, a make-up lotion, an astringent lotion, an emulsion, a moisturizing lotion, a nourishing lotion, a massage cream, a nourishing cream, a moisturizing cream, a hand cream, a foundation, an essence, a nourishing essence, a mask, a soap, a cleansing foam, a cleansing milk, a cleansing cream, a body milk, and a body wash may be prepared, without being limited thereto.

When the dosage form of the present invention is a paste, cream or gel, animal fibers, plant fibers, waxes, paraffins, starch, tragacanth, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide and the like may be used as carrier ingredients.

When the formulation of the present invention is a powder or a spray, lactose, talc, silicon dioxide, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier ingredient, and particularly, when the formulation is a spray, a propellant such as chlorofluorocarbon, propane/butane or dimethyl ether may be further contained.

When the formulation of the present invention is a solution or emulsion, a solvent, a solvating agent or an opacifying agent, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylene glycol oil, glycerol fatty acid ester, polyethylene glycol or fatty acid ester of sorbitan is used as a carrier component.

When the dosage form of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, or the like can be used as a carrier ingredient.

When the formulation according to the present invention is a surfactant-containing cleansing agent, fatty alcohol sulfate, fatty alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazoline derivative, methyltaurate, sarcosinate, fatty acid amide ether sulfate, alkylamide betaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, ethoxylated glycerin fatty acid ester, or the like can be used as a carrier ingredient.

Examples

Hereinafter, the present invention will be described in more detail with reference to examples. These examples are only examples to illustrate the present invention, and it should be understood by those skilled in the art that these examples do not limit the scope of the present invention.

[ example 1 ] preparation of Mixed extract

Soaking fructus Rosae Davuricae and semen Sesami Niger in water, taking out from the water, germinating at room temperature, and drying. After drying, the germinated fructus Rosae Davuricae and germinated semen Sesami Niger are mixed with the mume fructus, the quince fructus and the pine nut, and then extracted with 70% (v/v) ethanol as an extraction solvent at 50 to 55 deg.C, and filtered and concentrated to prepare a mixed extract (refer to FIG. 1).

[ Experimental example 1 ] evaluation of cytotoxicity

CCK-8 analysis was performed on human fibroblasts to evaluate the cytotoxicity of the mixed extract prepared in said example 1. CCK-8 analysis was performed according to the conventional method.

Each group was tested at 37 ℃ with 5% CO, n-4₂Human fibroblasts (HDFn) were cultured in a DMEM (Dulbecco's Modified Eagle's Medium) Medium containing 10% fetal bovine serum (FBS, Gibco) under an incubator (incubator).

Fibroblasts were treated with the mixed extract of example 1 at a concentration of 6ppm to 100ppm for 18 hours, and a non-treated group not treated with the mixed extract was used as a Control group (Control: CON) to compare cell survival rates and is shown in FIG. 2. As a result, since the mixed extract showed no cytotoxicity at all concentrations treated with the mixed extract, it was confirmed that the mixed extract of example 1 had no cytotoxicity.

[ Experimental example 2 ] evaluation of efficacy in improving skin dullness

Aminoguanidine (Aminoguanidine: AG) having pigmentation-improving efficacy was used as a positive control group to evaluate the skin-dullness-improving efficacy of the mixed extract of example 1. Aminoguanidine is known as an effective ingredient for improving pigmentation, dullness of skin according to patent document JP2004-509912a, and the content of said document is incorporated in its entirety into the present application by reference.

A collagen matrix supplemented with fibroblasts (HDFn) was prepared in a 48-well plate, and after treatment with the sample, fibroblasts were cultured in the collagen matrix for 7 days (culture conditions; 37 ℃ C., 5% CO)₂The incubator of (1). The sample treatments were a group treated with 30mM ribose (Sigma aldrich, Cat. No. R9629) alone, a group treated with 30mM ribose and 20mM aminoguanidine (Sigma aldrich, Cat. No. A7009), and a group treated with 30mM ribose and 12ppm to 50ppm of the mixed extract of the example 1. After 7 days of treatment of the fibroblasts with the specimen, autofluorescence values were measured at specific wavelengths in which substances decreased by the treatment of aminoguanidine, which has the effect of improving pigmentation, specifically react to compare the effects of improving skin dullness. The autofluorescence (autofluorescence) was measured by using a microplate reader (product of BioTek) under fluorescence measurement conditions with excitation light (excitation) at 340nm and emission light (emission) at 440 nm.

Each set was tested at n-6, as shown in fig. 3, with various graphs showing the results of the experiments, and the average value for each set is shown by the solid line (-).

When fibroblasts were cultured in a collagen matrix by treatment with Ribose (Ribose: Rib) as a stimulus, it was confirmed that Ribose increased the production of a substance that exhibits autofluorescence at 340nm of excitation light and 440nm of emission light. Specifically, the autofluorescence intensity of ribose was increased by about 132% compared with that of the non-treated group (control group: CON). In addition, aminoguanidine, which has the effect of improving pigmentation, decreases the autofluorescence intensity due to the increase of ribose by about 54.0%. Thus, in the present specification, a substance that exhibits autofluorescence at the excitation light of 340nm and the emission light of 440nm is referred to as a "skin browning component".

As shown in fig. 3, it was confirmed that the autofluorescence intensity increased by ribose was also decreased when the fibroblasts were treated with the mixed extract of example 1. It is understood that the mixed extract of example 1 has the effects of inhibiting the formation of skin browning components and improving skin dullness, as with aminoguanidine. In addition, when fibroblasts were treated with the mixed extract of example 1 described above at a concentration of more than 25ppm, the reduction rate of the skin browning components was increased, thereby confirming that the effect of improving skin dullness was excellent at a certain concentration or more. When fibroblasts were treated with the mixed extract at a concentration of 50ppm, the autofluorescence intensity was reduced by about 29.4% compared to the group treated with ribose alone.

[ Experimental example 3 ] evaluation of efficacy in improving skin transparency

Experimental products comprising the mixed extracts of example 1 were prepared according to the compositions of table 1, and the efficacy of improving skin transparency was evaluated as follows.

[ TABLE 1 ]

The experimental product was applied twice daily to the entire face for 8 weeks to 21 women between the ages of 30 and 50. In addition, the product was administered by radio scan before, after 4 weeks, and after 8 weeks, respectively^TMThe skin transparency of the right cheek front area was measured at an angle of 60 ° for an incident angle and 60 ° for a reflection angle under the same environmental conditions (truesystems, korea), and analyzed by a diffuse reflection (diffuse reflection) value. Skin light inside the skin with high transparency when light is incident on the skinThe reflection (diffuse reflection) will be relatively high.

The change in skin transparency after 8 cycles of use of the experimental product was analyzed by the value of the diffuse reflectance inside the skin and is shown in table 2 and fig. 4. As can be seen from the results, the average value of diffuse reflectance was significantly increased after the use of the experimental product, and thus the skin transparency was improved after 4 weeks and after 8 weeks. Thus confirming that the mixed extract according to the present specification has a significant effect of improving skin transparency.

[ TABLE 2 ]

(p < 0.05;. p <0.001vs. before use)

Hereinafter, dosage form examples of the composition according to one aspect of the present specification will be described, but it may be applied to various other dosage forms, which are only intended to describe the present invention in detail, not to limit the present invention.

Formulation example 1 astringent

Lotions were prepared according to the conventional method with the composition shown in table 3 below.

[ TABLE 3 ]

Formulation of ingredients	Content (wt%)
		Mixed extract of example 1	0.5
Glycerol	3.5
		Butanediol	2.0
Propylene glycol	2.0
		Oleyl alcohol	1.5
Ethanol	5.5
		Polysorbate 80	3.2
Carboxyvinyl polymer	0.1
		Antiseptic, pigment, and perfume	Proper amount of
Purified water	Balance of
		Total up to	100

Dosage form example 2 emulsions

Emulsions were prepared according to conventional methods with the compositions shown in table 4 below.

[ TABLE 4 ]

[ dosage form example 3 ] essence

Essences were prepared according to the composition shown in the following table 5 according to a conventional method.

[ TABLE 5 ]

Dosage form example 4 cream

The cream was prepared according to the conventional method with the composition shown in the following table 6.

[ TABLE 6 ]

Formulation of ingredients	Content (wt%)
		Mixed extract of example 1	0.5
Glycerol	3.5
		Butanediol	3.0
Liquid paraffin	7.0
		Beta-glucan	7.0
Carbomer	0.1
		Caprylic/capric triglyceride	3.0
Squalane	5.0
		Cetearyl glucoside	1.5
Sorbitan stearate	0.4
		Polysorbate 60	1.2
Triethanolamine	0.1
		Antiseptic, pigment, and perfume	Proper amount of
Purified water	Balance of
		Total up to	100

Dosage form example 5 gel

Gels were prepared according to conventional methods, with the compositions shown in table 7 below.

[ TABLE 7 ]

Formulation of ingredients	Content (wt%)
		Mixed extract of example 1	0.5
Cetearyl glucoside	1.5
		Cetyl octanoate	1.0
Glycerol	5.0
		Jojoba wax	3.0
Panthenol (DL-Panthenol)	1.0
		Ethanol	7.0
Carboxyvinyl polymer	0.6
		Propylene glycol	3.0
Antiseptic, pigment, and perfume	Proper amount of
		Purified water	Balance of
Total up to	100

Dosage form example 6 facial mask

The mask was prepared according to the conventional method with the composition shown in the following table 8.

[ TABLE 8 ]

As described above, having described certain portions of the present disclosure in detail, it should be understood by those skilled in the art that these specific techniques are only preferred embodiments and the scope of the present invention is not limited thereto. Therefore, the scope of the invention is to be defined by the appended claims and equivalents thereof.