阅读说明：本技术 一种增强免疫促生长的鲈鱼用复合饲料添加剂制备和应用 (Preparation and application of compound feed additive for enhancing immunity and promoting growth of weever ) 是由 杨星 张美彦 于 2020-09-08 设计创作，主要内容包括：本发明提供一种增强免疫促生长的鲈鱼用复合饲料添加剂制备和应用,涉及水产动物饲料添加剂领域。该一种具有增强免疫、促生长功能的鲈鱼用复合饲料添加剂制备和应用,其组成为田七、五倍子、黄芪、金银花、大青叶、维生素C、乳酸菌和酶制剂,包括以下步骤：S1：选料,S2：粉碎,S3：混合搅拌,S4：真空包装。通过田七、五倍子、黄芪、金银花、大青叶、维生素C、乳酸菌和酶制剂,有效提高养殖鲈鱼的生长速度,同时可有效增强鲈鱼的免疫功能,具有显著的保肝护胆功效。(The invention provides preparation and application of a compound feed additive for weever, which can enhance immunity and promote growth, and relates to the field of aquatic animal feed additives. The preparation and application of the compound feed additive for weever with the functions of enhancing immunity and promoting growth comprise pseudo-ginseng, Chinese gall, astragalus mongholicus, honeysuckle, folium isatidis, vitamin C, lactic acid bacteria and an enzyme preparation, and comprise the following steps: s1: selecting materials, S2: pulverization, S3: mixing and stirring, S4: and (7) vacuum packaging. The growth speed of the cultured weever is effectively improved through the pseudo-ginseng, the gallnut, the astragalus, the honeysuckle, the dyers woad leaf, the vitamin C, the lactic acid bacteria and the enzyme preparation, and meanwhile, the immune function of the weever can be effectively enhanced, so that the weever has remarkable effects of protecting the liver and the gallbladder.)

1. Preparation and application of a compound feed additive for weever for enhancing immunity and promoting growth are characterized in that: the composition comprises pseudo-ginseng, gallnut, astragalus, honeysuckle, folium isatidis, vitamin C, lactic acid bacteria and an enzyme preparation.

2. The preparation and application of the compound feed additive for weever for enhancing immunity and promoting growth according to claim 1 are characterized in that: the method comprises the following steps:

s1: sorting

Selecting 30-35 parts of pseudo-ginseng, 15-20 parts of Chinese gall, 15-20 parts of astragalus, 10-15 parts of honeysuckle, 5-10 parts of folium isatidis, 10-20 parts of vitamin C, 3-5 parts of lactic acid bacteria and 2-3 parts of an enzyme preparation.

S2: pulverizing

Respectively putting 30-35 parts of pseudo-ginseng, 15-20 parts of Chinese gall, 15-20 parts of astragalus membranaceus, 10-15 parts of honeysuckle and 5-10 parts of folium isatidis into a traditional Chinese medicine crusher, controlling the crushing speed of the traditional Chinese medicine crusher to be 30-40r/s, enabling the crushing time to be 10-15 minutes, respectively putting the crushed raw materials into a 100-mesh sample sieve, crushing while selecting impurities in the raw materials, enabling all the raw materials to pass through the 100-mesh sample sieve, and when a small part of the raw materials cannot pass through the 100-mesh sample sieve, re-putting the small part into the traditional Chinese medicine crusher again, and enabling all the raw materials to pass through the 100-mesh sample sieve;

s3: mixing and stirring

Respectively adding 30-35 parts of pseudo-ginseng, 15-20 parts of Chinese gall, 15-20 parts of astragalus membranaceus, 10-15 parts of honeysuckle and 5-10 parts of folium isatidis into a stirrer in sequence for stirring, so that the adding time interval is controlled to be 5 minutes, when the pseudo-ginseng, the Chinese gall, the astragalus membranaceus, the honeysuckle and the folium isatidis are all added into the stirrer, the mixing and stirring time of the raw materials is controlled to be 30 minutes, then simultaneously adding 10-20 parts of vitamin C, 3-5 parts of lactic acid bacteria and 2-3 parts of an enzyme preparation, so that the stirring speed is controlled to be 20-30r/s, so that the mixing time is 35-40 minutes, and then packaging;

s4: vacuum package

Packaging with 20 jin/bag by using a laminating paper bag to obtain the compound feed additive for weever with the functions of enhancing immunity and promoting growth.

3. The preparation and application of the compound feed additive for weever with the functions of enhancing immunity and promoting growth according to claim 1 are characterized in that: the compound feed additive is powder.

4. The preparation and application of the compound feed additive for weever for enhancing immunity and promoting growth according to claim 1 are characterized in that: the pseudo-ginseng, the gallnut, the astragalus, the honeysuckle and the folium isatidis are all traditional Chinese medicine raw materials.

5. The preparation and application of the compound feed additive for weever for enhancing immunity and promoting growth according to claim 1 are characterized in that: the vitamin C, the lactic acid bacteria and the enzyme preparation are pure substances with the concentration of 100%, and the lactic acid bacteria and the enzyme preparation are dry powders, so that the substances used in the actual preparation process have certain purity and can be converted into the actual substance using amount according to the actual content.

6. The preparation and application of the compound feed additive for weever for enhancing immunity and promoting growth according to claim 1 are characterized in that: the laminating paper bag is a vacuum sterile packaging bag.

Technical Field

The invention relates to the technical field of aquatic animal feed additives, in particular to preparation and application of a compound feed additive for weever, which has the functions of enhancing immunity and promoting growth.

Background

With the rapid development of the weever culture industry in China, a high-density intensive culture mode becomes the current main culture mode for culturing weever. The weever belongs to carnivorous fish, can eat the compound feed after the artificial domestication, the weever cultured artificially has the reasons of accelerated growth speed, unbalanced nutrition in the feed, improper use of low-quality feed, deterioration of culture water environment and the like, under the culture mode, the weever generally has liver and gall syndrome, fatty liver and the like caused by liver metabolic dysfunction, is in a sub-health state for a long time, has poor anti-stress capability, is easy to suffer from diseases, is easy to die in a large scale in a high-temperature season, and the like, and the cultured weever dies greatly in every year due to the liver metabolic dysfunction, directly or indirectly causes huge economic loss, thereby becoming a restriction factor for the healthy development of the weever culture industry in China.

At present, the development of healthy aquaculture is vigorously advocated in China, wherein healthy aquaculture aims at protecting the health of aquaculture animals and human beings and producing safe and nutritional aquatic products. Therefore, the team tests the influence of the pseudo-ginseng, the gallnut, the astragalus mongholicus, the honeysuckle, the folium isatidis, the vitamin C, the lactic acid bacteria and the enzyme preparation on the health and growth of the weever to form the compound feed additive with the function of enhancing immunity and promoting the healthy growth of the weever.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides the preparation and the application of the compound feed additive for the weever, which can enhance the immunity and promote the growth, improve the growth speed of the cultured weever, effectively enhance the immunity of the weever and have obvious effects of protecting liver and gallbladder.

(II) technical scheme

In order to achieve the purpose, the invention is realized by the following technical scheme: preparation and application of compound feed additive for weever for enhancing immunity and promoting growth are disclosed, which comprises Notoginseng radix, Galla chinensis, radix astragali, flos Lonicerae, folium Isatidis, vitamin C, lactobacillus and enzyme preparation.

Preferably, the preparation and application of the compound feed additive for weever with immunity enhancing and growth promoting functions according to claim 1 comprises the following steps:

s1: sorting

Selecting 30-35 parts of pseudo-ginseng, 15-20 parts of Chinese gall, 15-20 parts of astragalus, 10-15 parts of honeysuckle, 5-10 parts of folium isatidis, 10-20 parts of vitamin C, 3-5 parts of lactic acid bacteria and 2-3 parts of an enzyme preparation.

S2: pulverizing

Respectively putting 30-35 parts of the dried raw materials of pseudo-ginseng, 15-20 parts of Chinese gall, 15-20 parts of astragalus, 10-15 parts of honeysuckle and 5-10 parts of folium isatidis into a traditional Chinese medicine grinder, controlling the grinding speed of the traditional Chinese medicine grinder to be 30-40r/s, enabling the grinding time to be 10-15 minutes, respectively putting the ground raw materials into a 100-mesh sample sieve, grinding while selecting impurities in the raw materials, enabling all raw materials in a raw material library to pass through the 100-mesh sample sieve, and when a small part of raw materials cannot pass through the 100-mesh sample sieve, re-putting the small part into the traditional Chinese medicine grinder, and enabling all raw materials to pass through the 100-mesh sample sieve;

s3: mixing and stirring

Respectively adding 30-35 parts of pseudo-ginseng, 15-20 parts of Chinese gall, 15-20 parts of astragalus membranaceus, 10-15 parts of honeysuckle and 5-10 parts of folium isatidis into a stirrer in sequence for stirring, so that the adding time interval is controlled to be 5 minutes, when the pseudo-ginseng, the Chinese gall, the astragalus membranaceus, the honeysuckle and the folium isatidis are all added into the stirrer, the mixing and stirring time of the raw materials is controlled to be 30 minutes, then simultaneously adding 10-20 parts of vitamin C, 3-5 parts of lactic acid bacteria and 2-3 parts of an enzyme preparation, so that the stirring speed is controlled to be 20-30r/s, so that the mixing time is 35-40 minutes, and then packaging;

s4: vacuum package

Packaging with 20 jin/bag by using a laminating paper bag to obtain the compound feed additive for weever with the functions of enhancing immunity and promoting growth.

Preferably, the compound feed additive is powder.

Preferably, the pseudo-ginseng, the gallnut, the astragalus membranaceus, the honeysuckle and the folium isatidis are all traditional Chinese medicine raw materials.

Preferably, the vitamin C, the lactic acid bacteria and the enzyme preparation are 100% pure substances, and the lactic acid bacteria and the enzyme preparation are dry powders, and substances used in the actual preparation process have certain purity and can be converted into the actual substance using amount according to the actual content.

Preferably, the laminated paper bag is a vacuum sterile packaging bag.

(III) advantageous effects

The invention provides a preparation method and application of a compound feed additive for weever, which can enhance immunity and promote growth. The method has the following beneficial effects:

1. the formula is reasonable, the use is convenient, the additive can effectively improve the growth speed of cultured weever, can effectively enhance the immune function of the weever, and has obvious effects of protecting liver and gallbladder.

2. In the invention, the Chinese herbal medicines used in the feed additive are all low-grade raw Chinese herbal medicines, and can be directly mixed with other raw materials only by crushing and sieving with a sieve, and the method is simple and easy to use.

3. According to the compound feed additive, the immunity of the weever can be effectively enhanced, the occurrence of diseases is reduced, the growth speed of the weever can be obviously improved, the compound feed additive has the effect of protecting the liver and the gallbladder of the weever, can protect liver injury caused by toxic and harmful substances, has the detoxifying effect on poisoning caused by drugs, food, in-vivo metabolites and bacterial toxins, and can promote the digestion and absorption of the food, reduce the feed coefficient, reduce the feeding cost and maintain the health of the weever.

4. The invention effectively relieves the problem of using a large amount of antibiotics due to diseases, and the used raw materials are cheap and easily available, safe and efficient, and convenient to use and popularize.

Detailed Description

The following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The first embodiment is as follows:

the embodiment of the invention provides preparation and application of a compound feed additive for weever, which is used for enhancing immunity and promoting growth, and comprises pseudo-ginseng, gallnut, astragalus mongholicus, honeysuckle, folium isatidis, vitamin C, lactic acid bacteria and an enzyme preparation.

The preparation and application of the compound feed additive for the weever with the functions of enhancing immunity and promoting growth comprise the following steps:

s1: sorting

Preparing 100 parts of compound feed additive, which comprises the following raw materials: selecting 30 parts of pseudo-ginseng, 15 parts of Chinese gall, 15 parts of astragalus, 10 parts of honeysuckle, 5 parts of folium isatidis, 20 parts of vitamin C, 3 parts of lactic acid bacteria and 2 parts of an enzyme preparation.

S2: pulverizing

Respectively putting 30 parts of the raw materials of pseudo-ginseng, 15 parts of Chinese gall, 15 parts of astragalus membranaceus, 10 parts of honeysuckle and 5 parts of folium isatidis after being dried in the first step into a traditional Chinese medicine grinder, then controlling the grinding speed of the traditional Chinese medicine grinder to be 30r/s, enabling the grinding time to be 10 minutes, then respectively putting the ground raw materials into a 100-mesh sample sieve, grinding while selecting impurities in the raw materials, enabling the raw material library to completely pass through the 100-mesh sample sieve, and when a small part of raw materials cannot pass through the 100-mesh sample sieve, re-putting the small part into the traditional Chinese medicine grinder, and enabling the raw materials to completely pass through the 100-mesh sample sieve;

s3: mixing and stirring

Respectively adding 30 parts of pseudo-ginseng, 15 parts of Chinese gall, 15 parts of astragalus, 10 parts of honeysuckle and 5 parts of folium isatidis into a stirrer for stirring, controlling the adding time interval to be 5 minutes, controlling the mixing and stirring time of the raw materials to be 30 minutes when the pseudo-ginseng, the Chinese gall, the astragalus, the honeysuckle and the folium isatidis are all added into the stirrer, then simultaneously adding 20 parts of vitamin C, 3 parts of lactic acid bacteria and 2 parts of an enzyme preparation, controlling the stirring speed to be 30r/s, controlling the mixing time to be 40 minutes, and then packaging;

s4: vacuum package

Packaging with 20 jin/bag by using a laminating paper bag to obtain the compound feed additive for weever with the functions of enhancing immunity and promoting growth.

The compound feed additive is powder, pseudo-ginseng, Chinese gall, astragalus, honeysuckle and indigowoad leaf are all traditional Chinese medicine raw material medicines, vitamin C, lactic acid bacteria and an enzyme preparation are 100% pure substances, the lactic acid bacteria and the enzyme preparation are dry powders, substances used in the actual preparation process have certain purity and can be converted into the use amount of actual substances according to the actual content, and the laminating paper bag is a vacuum sterile packaging bag.

A use method of the compound feed additive for the weever for enhancing immunity and promoting growth comprises the following steps: the health care dosage is added into the feed according to the adding proportion of 1 percent of the total weight of the feed, the dosage is added into the feed according to the adding proportion of 3 percent of the total weight of the feed in seasons with frequent summer high-temperature diseases, and the feed is a special compound feed for weever produced by manufacturers.

Growth test: the weever is provided by the fishery of Zunyi Monhong ecological agriculture development limited company. The test selects healthy, sized, and substantially consistent weight weever, the initial weight of the individual is about 20 grams. Randomly grouping, wherein an experimental group and a control group are arranged, each group is provided with 3 parallel tests, and each parallel group comprises 50 test fishes, and 6 net cages (the specification is 250cm multiplied by 150cm multiplied by 100cm) in total. After 7 days of acclimation of the weever, feeding 1.0 percent of compound feed additive for 20 days, and determining the growth influence.

The results show that compared with a control group, the growth speed and the weight gain rate of the 1.0 percent compound feed additive are obviously improved by 4.56 percent and 11.28 percent (P <0.05), and simultaneously the concentration of blood cortisol is obviously reduced by 6.45 percent, the blood sugar level is 3.35 percent and the concentration of malonaldehyde is 8.74 percent (P <0.05), so that the compound feed additive can improve the growth speed of a fish body, can effectively enhance the immune function of the fish body and has obvious liver and gallbladder protecting effects.

Example two:

the embodiment of the invention provides preparation and application of a compound feed additive for weever, which is used for enhancing immunity and promoting growth, and comprises pseudo-ginseng, gallnut, astragalus mongholicus, honeysuckle, folium isatidis, vitamin C, lactic acid bacteria and an enzyme preparation.

The preparation and application of the compound feed additive for the weever for enhancing immunity and promoting growth comprise the following steps:

s1: sorting

Preparing 100 parts of compound feed additive, which comprises the following raw materials: selecting 35 parts of pseudo-ginseng, 15 parts of Chinese gall, 15 parts of astragalus, 12 parts of honeysuckle, 5 parts of folium isatidis, 10 parts of vitamin C, 5 parts of lactic acid bacteria and 3 parts of enzyme preparation.

S2: pulverizing

Respectively putting 35 parts of the raw materials of pseudo-ginseng, 15 parts of Chinese gall, 15 parts of astragalus membranaceus, 12 parts of honeysuckle and 5 parts of folium isatidis after drying in the first step into a traditional Chinese medicine grinder, then controlling the grinding speed of the traditional Chinese medicine grinder to be 40r/s, enabling the grinding time to be 15 minutes, then respectively putting the ground raw materials into a 100-mesh sample sieve, grinding while selecting impurities in the raw materials, enabling the raw material library to completely pass through the 100-mesh sample sieve, and when a small part of raw materials cannot pass through the 100-mesh sample sieve, re-putting the small part into the traditional Chinese medicine grinder, and enabling the raw materials to completely pass through the 100-mesh sample sieve;

s3: mixing and stirring

Respectively adding 35 parts of pseudo-ginseng, 15 parts of Chinese gall, 15 parts of astragalus, 12 parts of honeysuckle and 5 parts of folium isatidis into a stirrer for stirring, controlling the adding time interval to be 5 minutes, controlling the mixing and stirring time of the raw materials to be 30 minutes when the pseudo-ginseng, the Chinese gall, the astragalus, the honeysuckle and the folium isatidis are all added into the stirrer, then simultaneously adding 10 parts of vitamin C, 5 parts of lactic acid bacteria and 3 parts of an enzyme preparation, controlling the stirring speed to be 20r/s, controlling the mixing time to be 35 minutes, and then packaging;

s4: vacuum package

Packaging with 20 jin/bag by using a laminating paper bag to obtain the compound feed additive for weever with the functions of enhancing immunity and promoting growth.

The compound feed additive is powder, pseudo-ginseng, Chinese gall, astragalus, honeysuckle and indigowoad leaf are all traditional Chinese medicine raw material medicines, vitamin C, lactic acid bacteria and an enzyme preparation are 100% pure substances, the lactic acid bacteria and the enzyme preparation are dry powders, substances used in the actual preparation process have certain purity and can be converted into the use amount of actual substances according to the actual content, and the laminating paper bag is a vacuum sterile packaging bag.

A use method of the compound feed additive for the weever for enhancing immunity and promoting growth comprises the following steps: the health care dosage is added into the feed according to the adding proportion of 1 percent of the total weight of the feed, the dosage is added into the feed according to the adding proportion of 3 percent of the total weight of the feed in seasons with frequent summer high-temperature diseases, and the feed is a special compound feed for weever produced by manufacturers.

Growth test: the weever is provided by the fishery of Zunyi Monhong ecological agriculture development limited company. The test selects healthy, sized, and substantially consistent weight weever, the initial weight of the individual is about 20 grams. Randomly grouping, wherein an experimental group and a control group are arranged, each group is provided with 3 parallel tests, and each parallel group comprises 50 test fishes, and 6 net cages (the specification is 250cm multiplied by 150cm multiplied by 100cm) in total. After 7 days of acclimation of the weever, feeding 3.0 percent of compound feed additive for 20 days, and determining the growth influence.

The results show that compared with a control group, the 3.0 percent compound feed additive has the advantages that the growth speed and the weight gain rate are obviously improved by 5.66 percent and 13.48 percent (P is less than 0.05), and simultaneously, the blood cortisol concentration is obviously reduced by 7.55 percent, the blood sugar level is 4.45 percent and the malonaldehyde concentration is 9.56 percent (P is less than 0.05), so that the compound feed additive can improve the growth speed of a fish body, can effectively enhance the immune function of the fish body, and has obvious liver and gallbladder protecting effects.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.