阅读说明：本技术 一种em菌洗衣液及其制备方法 (EM (effective microorganisms) bacterium laundry detergent and preparation method thereof ) 是由 聂绪明 聂啸 于 2020-12-17 设计创作，主要内容包括：本发明公开了一种EM菌洗衣液,包括以下重量份的原料：EM菌液50-70份、非离子表面活性剂40-60份、壳聚糖季铵盐10-20份、增稠剂5-8份、复合酶制剂1-3份、pH调节剂1-3份和香精0.4-0.8份；制备方法：(1)称取各原料；(2)先将EM菌液加热至35-45℃；(3)在搅拌状态下,依次加入非离子表面活性剂和壳聚糖季铵盐,降温至20-25℃；(4)依次加入增稠剂、复合酶制剂、pH调节剂和香精,搅拌至混合均匀,脱气消泡,即得。本发明EM菌洗衣液去污能力强,抑菌效果好,泡沫丰富,易冲洗,而且性质温和,保护纤维抗静电,无磷不刺激,自然花香,清馨宜人,持久散发。(The invention discloses an EM (effective microorganism) bacterium laundry detergent which comprises the following raw materials in parts by weight: 50-70 parts of EM bacterial liquid, 40-60 parts of non-ionic surfactant, 10-20 parts of chitosan quaternary ammonium salt, 5-8 parts of thickening agent, 1-3 parts of complex enzyme preparation, 1-3 parts of pH regulator and 0.4-0.8 part of essence; the preparation method comprises the following steps: (1) weighing the raw materials; (2) heating EM bacterial liquid to 35-45 ℃; (3) sequentially adding a nonionic surfactant and chitosan quaternary ammonium salt under stirring, and cooling to 20-25 ℃; (4) sequentially adding the thickening agent, the complex enzyme preparation, the pH regulator and the essence, stirring until the mixture is uniformly mixed, degassing and defoaming to obtain the composite enzyme. The EM bacterial laundry detergent disclosed by the invention has the advantages of strong decontamination capability, good antibacterial effect, abundant foam, easiness in washing, mild property, capability of protecting fiber from static electricity, no phosphorus, no irritation, natural fragrance, delightness and lasting emission.)

1. The EM bacterial laundry detergent is characterized by comprising the following raw materials in parts by weight: 50-70 parts of EM bacterial liquid, 40-60 parts of non-ionic surfactant, 10-20 parts of chitosan quaternary ammonium salt, 5-8 parts of thickening agent, 1-3 parts of complex enzyme preparation, 1-3 parts of pH regulator and 0.4-0.8 part of essence.

2. The EM bacterial liquid laundry detergent of claim 1, wherein the viable bacteria content of the EM bacterial liquid is more than or equal to 1.0 x 10¹⁰cfu/g。

3. The EM bacterial laundry detergent of claim 1, wherein the non-ionic surfactant is at least one of polyoxyethylene fatty acid ester, polyoxyethylene alkylamine and polyoxyethylene alkylolamide.

4. The EM bacteria laundry detergent of claim 1, wherein the thickener is at least one of xanthan gum, carrageenan and sodium chloride.

5. The EM bacterial laundry detergent of claim 1, wherein the complex enzyme preparation is prepared from (1-3) of: (1-2): and (1-2) mixing the neutral protease, the alpha-amylase and the cellulase.

6. The EM bacteria laundry detergent of claim 1, wherein the pH regulator is citric acid and/or sodium citrate.

7. The EM bacterial laundry detergent of claim 1, wherein the essence is at least one of lavender essence, jasmine essence and rose essence.

8. A preparation method of an EM (effective microorganism) bacterium laundry detergent is characterized by comprising the following steps:

(1) weighing the raw materials according to the parts by weight of the EM bacterial laundry detergent as defined in any one of claims 1-7;

(2) heating EM bacterial liquid to 35-45 ℃ to obtain a material A;

(3) under the stirring state, sequentially adding a nonionic surfactant and chitosan quaternary ammonium salt into the material A, and cooling to 20-25 ℃ to obtain a material B;

(4) and (3) sequentially adding a thickening agent, a complex enzyme preparation, a pH regulator and essence into the material B, stirring until the materials are uniformly mixed, degassing and defoaming to obtain the EM bacterial laundry detergent.

9. The method for preparing the EM bacterial liquid laundry detergent according to claim 8, wherein in the step (2), the method for preparing the EM bacterial liquid comprises the following steps: adding EM strain, brown sugar and salt into sterile water, and fermenting in a sealed condition at 35-37 deg.C for 1-2 days to obtain EM liquid strain;

and then adding the EM liquid strain and brown sugar into sterile water, and fermenting in a sealed manner for 1-3 days at the temperature of 35-37 ℃ to obtain the EM bacterial liquid.

10. The method for preparing the EM bacterial laundry detergent as claimed in claim 8, wherein in the step (3), the stirring speed is 80-120 r/min;

in the step (4), the stirring speed is 100-200r/min, and the time is 30-60 min.

Technical Field

The invention relates to the technical field of daily chemical industry, in particular to a laundry detergent, and more particularly relates to an EM (effective microorganism) laundry detergent and a preparation method thereof.

Background

The laundry detergent market in China is increasing at a rate of 27.2% per year, and the compound increase of 3 years is over 100%; meanwhile, the annual average acceleration rate of the washing powder market is only 2.2%. The working principle of the laundry detergent is the same as that of the traditional washing powder and soap, and the active ingredients are all surfactants, and the difference is that: the traditional washing powder and soap adopt anionic surfactants which mainly comprise alkyl sodium sulfonate and sodium stearate, and have strong alkalinity (the pH of the washing powder is generally more than 12), so that the washing powder and soap have large irritation and damage to the skin when in use; the laundry detergent mostly adopts nonionic surfactants, the pH value of the laundry detergent is close to neutral, the laundry detergent is mild to the skin, the structure of the laundry detergent comprises a hydrophilic end and a lipophilic end, the lipophilic end is combined with stains, then the stains are separated from the fabric through physical movement (such as hand rubbing and machine movement), meanwhile, the surfactants reduce the tension of water, the water can reach the surface of the fabric, the effective components can play a role, and the laundry detergent is degraded more quickly after being discharged into the nature, so the laundry detergent becomes a new generation of detergent.

When the consumer purchases the laundry detergent, the following factors play a large role: 1. decontamination/whitening; 2. price/promotion; 3. fragrance/fresh fragrance; 4. care for clothes/softness; 5. is mild and does not hurt hands; 6. easy rinsing/water saving; 7. color protection/fastness; 8. low temperature/easy dissolution; 9. and (4) disinfection/sterilization. With the increase of health awareness of people, clothes cannot be cleaned only with cleaning efficacy, and sterilization and disinfection while clothes cleaning become the first choice of consumers.

However, functional laundry detergents with antibacterial and disinfecting functions are available in the market, the commonly added disinfecting components are chlorine-containing disinfectants, oxygen-containing disinfectants or alcohol-containing disinfectants, and both surfactants and disinfectants adopted by the laundry detergents have unpleasant pungent odor; it is irritant and corrosive to fabric and skin; the stability is poor; the compatibility with other raw materials is poor; the environment is not friendly, and the like, and the health is very easily injured.

Therefore, how to provide a green and environment-friendly laundry detergent with decontamination and bacteriostasis functions is a problem to be solved by those skilled in the art.

Disclosure of Invention

In view of the above, the present invention aims to provide an EM bacteria laundry detergent and a preparation method thereof, so as to solve the defects in the prior art.

In order to achieve the purpose, the invention adopts the following technical scheme:

the EM bacterial laundry detergent comprises the following raw materials in parts by weight: 50-70 parts of EM bacterial liquid, 40-60 parts of non-ionic surfactant, 10-20 parts of chitosan quaternary ammonium salt, 5-8 parts of thickening agent, 1-3 parts of complex enzyme preparation, 1-3 parts of pH regulator and 0.4-0.8 part of essence.

The invention has the beneficial effects that:

the EM bacteria is an English abbreviation of effective microbiota, is also called EM probiotic stock solution, is a microbial preparation compounded by 10 and more than 80 microorganisms which mainly comprise photosynthetic bacteria, lactic acid bacteria, saccharomycetes and actinomycetes, has an action mechanism of forming competition of nutrition competition of the EM bacteria and pathogenic microorganisms, thereby controlling the propagation of the pathogenic microorganisms, and has the advantages of complex structure, stable performance and complete functions. According to the invention, the EM bacterial liquid is creatively added into the laundry detergent, and probiotics such as lactobacillus and the like have strong sterilization capability, so that the activity of harmful microorganisms can be effectively inhibited, and clothes can be kept clean.

The chitosan quaternary ammonium salt is a cationic natural high molecular polymer prepared by chemically modifying chitosan, is totally called hydroxypropyl trimethyl ammonium chloride chitosan, is a white or light yellow powdery solid, has strong hygroscopicity and has refreshing sweet taste; the chitosan quaternary ammonium salt has good antibacterial property, film forming property, conditioning property, moisture absorption and retention property, biocompatibility, biodegradability, flocculation property, antistatic property and other properties; the chitosan quaternary ammonium salt has good water solubility, can be dissolved under both acidic and alkaline conditions, and has good solution stability.

Further, the viable bacteria content of the EM bacterial liquid is more than or equal to 1.0 multiplied by 10¹⁰cfu/g。

The beneficial effect of adopting the further technical scheme is that the live bacteria content of the EM bacterial liquid is higher, and the bacteriostatic and bactericidal effects of the EM bacteria can be fully exerted.

Further, the nonionic surfactant is at least one of polyoxyethylene fatty acid ester, polyoxyethylene alkylamine, and polyoxyethylene alkylolamide.

The nonionic surfactant has the advantages of high surface activity, good performances of solubilization, washing, calcium soap dispersion and the like, small irritation, excellent wetting and washing functions, sterilizing and antistatic capabilities and good biodegradability. Wherein, the polyoxyethylene fatty acid ester has good washing power and oil-soluble emulsifying power; the polyoxyethylene alkylamine is acid-resistant and has bactericidal performance; the polyoxyethylene alkylolamide has stronger foaming and foam stabilizing effects, and good washing power, solubilizing power and thickening effect.

Further, the thickener is at least one of xanthan gum, carrageenan and sodium chloride.

The further technical scheme has the beneficial effects that the thickening agent has multiple performances of thickening, foam stabilization, decontamination, emulsification, dispersion and the like, and has synergistic effects of reducing stimulation and the like. The xanthan gum is a microbial extracellular polysaccharide with wide effect produced by taking carbohydrates as main raw materials (such as corn starch) through fermentation engineering, has unique rheological property, good water solubility, heat and acid-base stability and good compatibility with various salts, and has the effects of resisting oxidation, preventing skin aging and the like; carrageenan is a hydrophilic colloid, has better effect than methylcellulose on preventing recontamination, and can improve the dispersion and storage performance of liquid detergent; the charged ions in the sodium chloride can compress the double electric layers of the micelles, and the mutual repulsion between surface active ions is reduced, so that more surface active ions enter the micelles, the aggregation number of the micelles is increased, and the solution becomes viscous.

Further, the complex enzyme preparation comprises the following components in percentage by mass (1-3): (1-2): and (1-2) mixing the neutral protease, the alpha-amylase and the cellulase.

The compound enzyme preparation prepared from the raw materials with the mass ratio has the beneficial effect that the compound enzyme preparation prepared from the raw materials with the mass ratio can improve the capacity of removing conventional stains. The neutral protease is obtained by fermenting and extracting bacillus subtilis, belongs to an endonuclease, and can help to remove other dirt mixed with protein; the alpha-1, 4-glucosidic bond in the alpha-amylase can cut a starch chain into short-chain dextrin, oligosaccharide and a small amount of maltose and glucose, and can decompose and remove starch stains; the cellulase is a collective name of a group of enzymes for degrading cellulose to generate glucose, is a complex enzyme, mainly comprises exo-beta-glucanase, endo-beta-glucanase, beta-glucosidase and the like, and can decompose and remove fiber stains.

Further, the pH regulator is citric acid and/or sodium citrate.

The pH regulator can regulate the pH value of the system to a proper range, so that the damage to the skin of the washed clothes is reduced.

Further, the essence is at least one of lavender essence, jasmine essence and rose essence.

The essence has the beneficial effects that the essence can make clothes to be washed emit attractive fragrance, is novel and lasting in fragrance.

A preparation method of an EM (effective microorganism) bacterium laundry detergent specifically comprises the following steps:

(1) weighing the raw materials according to the parts by weight of the EM bacterial laundry detergent;

(2) heating EM bacterial liquid to 35-45 ℃ to obtain a material A;

(3) under the stirring state, sequentially adding a nonionic surfactant and chitosan quaternary ammonium salt into the material A, and cooling to 20-25 ℃ to obtain a material B;

(4) and (3) sequentially adding the thickening agent, the complex enzyme preparation, the pH regulator and the essence into the material B, stirring until the materials are uniformly mixed, degassing and defoaming to obtain the EM bacteria laundry detergent.

The method has the advantages of simple operation, mild conditions and suitability for large-scale industrial production.

Further, in the step (2), the preparation method of the EM bacterial liquid comprises the following steps: adding EM strain, brown sugar and salt into sterile water, and fermenting in a sealed condition at 35-37 deg.C for 1-2 days to obtain EM liquid strain; then adding the EM liquid strain and brown sugar into sterile water, and fermenting in a sealed manner for 1-3 days at the temperature of 35-37 ℃ to obtain the EM bacterial liquid.

The beneficial effects of the further technical scheme are that the preparation method of the EM bacterial liquid is simple to operate, the prepared EM bacterial liquid has high viable bacteria content and good bacteriostatic effect.

Further, in the step (3), the stirring speed is 80-120 r/min; in the step (4), the stirring speed is 100-200rr/min, and the time is 30-60 min.

According to the technical scheme, compared with the prior art, the invention has the following beneficial effects:

according to the invention, the EM bacterial liquid, the chitosan quaternary ammonium salt, the complex enzyme preparation and other auxiliaries are reasonably compounded, so that the prepared EM bacterial laundry detergent has the advantages of strong decontamination capability, good antibacterial effect, rich foam, easiness in washing, mild property, fiber antistatic protection, no phosphorus or irritation, natural flower fragrance, delightness and delightness, and lasting emission.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the following examples, the viable bacteria content of the EM bacterial liquid was 1.0X 10¹⁰cfu/g。

Example 1

The EM bacterial laundry detergent comprises the following raw materials in parts by weight: 50g of EM bacterial liquid, 40g of polyoxyethylene fatty acid ester, 10g of chitosan quaternary ammonium salt, 4g of xanthan gum, 0.25g of neutral protease, 0.25g of alpha-amylase, 0.5g of cellulase, 1g of citric acid and 0.4g of lavender essence;

the preparation method of the EM bacterial laundry detergent is characterized by comprising the following steps:

(1) weighing the raw materials according to the weight of the EM bacterial laundry detergent;

(2) heating EM bacterial liquid to 35 ℃ to obtain a material A; the preparation method of the EM bacterial liquid comprises the following steps: adding EM strain, brown sugar and salt into sterile water, and fermenting at 35 deg.C for 1 day under sealed condition to obtain EM liquid strain; adding EM liquid strain and brown sugar into sterile water, and fermenting in a sealed manner at 35 ℃ for 1 day to obtain EM bacterial liquid;

(3) sequentially adding fatty acid polyoxyethylene ester and chitosan quaternary ammonium salt into the material A under the stirring state of 80r/min, and cooling to 20 ℃ to obtain a material B;

(4) and (3) adding xanthan gum, neutral protease, alpha-amylase, cellulase, citric acid and lavender essence into the material B in sequence, stirring at the speed of 100r/min for 30min until the materials are uniformly mixed, degassing and defoaming to obtain the EM (effective microorganism) laundry detergent.

Example 2

The EM bacterial laundry detergent comprises the following raw materials in parts by weight: 70g of EM bacterial liquid, 60g of polyoxyethylene alkylamine, 20g of chitosan quaternary ammonium salt, 8g of carrageenan, 1.5g of neutral protease, 1.0g of alpha-amylase, 0.5g of cellulase, 3g of citric acid and 0.8g of jasmine essence;

the preparation method of the EM bacterial laundry detergent is characterized by comprising the following steps:

(1) weighing the raw materials according to the weight of the EM bacterial laundry detergent;

(2) heating EM bacterial liquid to 45 ℃ to obtain a material A; the preparation method of the EM bacterial liquid comprises the following steps: adding EM strain, brown sugar and salt into sterile water, and fermenting in a sealed condition at 37 deg.C for 2 days to obtain EM liquid strain; adding EM liquid strain and brown sugar into sterile water, and fermenting in a sealed manner at 37 ℃ for 3 days to obtain EM bacterial liquid;

(3) sequentially adding polyoxyethylene alkylamine and chitosan quaternary ammonium salt into the material A under the stirring state of 120r/min, and cooling to 25 ℃ to obtain a material B;

(4) and sequentially adding carrageenan, neutral protease, alpha-amylase, cellulase, citric acid and jasmine essence into the material B, stirring at the speed of 200r/min for 60min until the materials are uniformly mixed, degassing and defoaming to obtain the EM bacteria laundry detergent.

Example 3

The EM bacterial laundry detergent comprises the following raw materials in parts by weight: 60g of EM bacterial liquid, 50g of polyoxyethylene alkylolamide, 15g of chitosan quaternary ammonium salt, 8g of sodium chloride, 1.2g of neutral protease, 0.4g of alpha-amylase, 0.4g of cellulase, 2g of sodium citrate and 0.6g of rose essence;

the preparation method of the EM bacterial laundry detergent is characterized by comprising the following steps:

(1) weighing the raw materials according to the weight of the EM bacterial laundry detergent;

(2) heating EM bacterial liquid to 40 ℃ to obtain a material A; the preparation method of the EM bacterial liquid comprises the following steps: adding EM strain, brown sugar and salt into sterile water, and fermenting in a closed manner at 36 deg.C for 2 days to obtain EM liquid strain; adding EM liquid strain and brown sugar into sterile water, and fermenting in a closed manner at 36 deg.C for 2 days to obtain EM bacterial liquid;

(3) under the stirring state of 100r/min, sequentially adding polyoxyethylene alkylolamide and chitosan quaternary ammonium salt into the material A, and cooling to 23 ℃ to obtain a material B;

(4) and (3) sequentially adding sodium chloride, neutral protease, alpha-amylase, cellulase, sodium citrate and rose essence into the material B, stirring at the speed of 150r/min for 45min until the materials are uniformly mixed, degassing and defoaming to obtain the EM (effective microorganism) laundry detergent.

Comparative example 1

The only difference from example 3 is that no EM bacteria were contained.

Comparative example 2

The only difference from example 3 is that the chitosan quaternary ammonium salt is not included.

Comparative example 3

The only difference from example 3 is that no neutral protease, no alpha-amylase and no cellulase are present.

Performance testing

1. Sensory index and physicochemical index test

The EM bacterial laundry detergents prepared in the examples 1-3 and the comparative examples 1-3 are respectively taken for carrying out sensory index and physicochemical index detection according to the test method and detection index of QB/T1224-2012 & lt & gt liquid detergent for clothing.

The results of the measurements are shown in tables 1 and 2.

TABLE 1 sensory index test results of EM bacterial laundry detergent in examples 1-3 and comparative examples 1-3

As can be seen from table 1, the EM bacteria laundry detergents prepared in examples 1 to 3 of the present invention are not layered, have no significant suspended matter or precipitate, have no uniform liquid of mechanical impurities, have no peculiar smell, and meet the specified odor type, all meeting the sensory index requirements of QB/T1224-2012 "liquid detergent for clothing".

Table 2 detection results of physical and chemical indexes of EM (effective microorganisms) laundry detergent in examples 1-3 and comparative examples 1-3

As can be seen from Table 2, the EM bacterial laundry detergent prepared in the embodiments 1-3 of the invention is placed at 40 +/-2 ℃ for 24h, and has no obvious change after being restored to room temperature and before the test; standing at-5 + -2 deg.C for 24h, and recovering to room temperature without obvious change; the total effective matter is not less than 55.96 percent, the pH (25 ℃) is 7.2-7.6, and the total phosphorus pentoxide content is zero, which all meet the physical and chemical index requirements of QB/T1224-.

2. Detergency test

Six pieces of carbon black oil stain cloth, protein stain cloth and sebum stain cloth with the same pollution degree are taken, the EM bacterial laundry detergent prepared in the examples 1-3 and the comparative examples 1-3 is used for carrying out a decontamination capability test according to GB/T13174-2008 'determination of decontamination capability and circulating washing performance of a detergent for clothing', and the decontamination capability test is taken as reference with a standard laundry detergent decontamination value (1) sold in the market.

The test results are shown in table 3.

TABLE 3 results of the detergency test on EM bacterial laundry detergents of examples 1 to 3 and comparative examples 1 to 3

Detecting items	Carbon black greasy dirt cloth	Protein dirty cloth	Sebum dirt cloth
				Example 1	1.52	1.48	1.64
Example 2	1.56	1.51	1.62
				Example 3	1.57	4.54	1.68
Comparative example 1	1.02	1.10	1.06
				Comparative example 2	1.08	1.04	1.12
Comparative example 3	1.01	1.01	1.11
				Standard commercial laundry detergent	1.00	1.00	1.00

As can be seen from Table 3, the EM (effective microorganisms) laundry detergents prepared in the examples 1-3 of the invention have stronger decontamination capability, and are far better than those prepared in the comparative examples 1-3 and commercially available laundry detergents.

3. Performance test for bacteriostatic ability

The EM bacterial laundry detergents prepared in examples 1-3 and comparative examples 1-3 are respectively taken for carrying out bacteriostatic ability tests according to QB/T2738-2012 evaluation method for antibacterial and bacteriostatic effects of daily chemical products.

The test results are shown in table 4.

TABLE 4 results of the test of bacteriostatic ability of EM bacterial laundry detergents of examples 1 to 3 and comparative examples 1 to 3

As can be seen from Table 4, the EM bacterial laundry detergent prepared in the examples 1-3 of the invention has the bacteriostatic ability of more than 99% on Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 15442, and is far superior to the EM bacterial laundry detergent prepared in the comparative examples 1-3.

Experiments 1 and 2 show that the EM bacterial liquid, the chitosan quaternary ammonium salt, the complex enzyme preparation and other additives are reasonably compounded, so that the prepared EM bacterial laundry detergent has the advantages of strong decontamination capability, good bacteriostatic effect, abundant foam, easiness in washing, mild property, capability of protecting fibers from static electricity, no phosphorus and no irritation, natural flower fragrance, delightness and delightness, and lasting emission.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.