阅读说明：本技术 一种血球分析仪的小孔堵孔识别方法和装置 (Small hole plugging identification method and device for blood cell analyzer ) 是由 王兴红 邹海涛 于 2020-11-17 设计创作，主要内容包括：本发明公开了一种小孔堵孔识别方法和装置,其中所述方法对小孔电压曲线进行求导,获取小孔电压曲线的拐点,并求取拐点间导函数的积分来识别堵孔状况,结合拐点位置获得堵孔发生或结束的时间点,当发现堵孔时还可结合预定时间段内小孔电压曲线的平均电压进一步判断堵孔工况,避免了堵孔不报和误报堵孔,从而能够更准确地识别小孔的堵孔工况。(The invention discloses a method and a device for identifying small hole plugging, wherein the method is used for deriving a small hole voltage curve, acquiring inflection points of the small hole voltage curve, and calculating integral of a derivative function between the inflection points to identify the plugging condition, and acquiring the time point of occurrence or ending of plugging by combining the positions of the inflection points.)

1. A method for identifying the blockage of a small hole of a blood cell analyzer is characterized by comprising the following steps:

acquiring voltage between electrodes on two sides of a small hole within a period of time to obtain a small hole voltage curve V ═ f (t), wherein V is a voltage value, and t is time;

derivative the voltage curve V ═ f (t) of the small hole to obtain its derivative functionAccording to derivative functionObtain the inflection point position A [ i ]]Wherein A [ i ]]Represents the position of the ith corner, i ∈ (1,2, ·, N);

calculating the integral of a derivative function between two adjacent inflection points by the formula

For all integrated values V_j(t) calculating the absolute value, taking the maximum value MAXV, MAXV ═ V_k(t)|，k∈(1,2,…,N/2)；

Comparing the maximum value MAXV of the integral absolute value with a first threshold, when MAXV is larger than the first threshold, judging that the small hole is blocked, wherein the inflection point position A < 2k > is a hole blocking starting point, the inflection point position A < 2k + 1> is a hole blocking end point, and executing a first hole blocking working condition identification program; when the MAXV is smaller than or equal to the first threshold value, executing a second hole plugging working condition identification program;

the first hole plugging condition identification program comprises:

when V is_k(t)<When 0, calculating the small hole voltage curve V ═ f (t) at the end point A [2k + 1-]And after the first preset time period, the average voltage V _ mean _ EP is obtained, when the V _ mean _ EP is greater than a second threshold value, the hole plugging working condition is judged to be full-course hole plugging, and when the V _ mean _ EP is less than or equal to the second threshold value, the hole plugging working condition is judged to be that the hole is plugged first and then is positiveFrequently;

when V is_k(t)>When 0, calculating the small hole voltage curve V ═ f (t) at the starting point A [2k ] of the hole plugging]The average voltage V _ mean _ SP in the second preset time period is judged to be full-course hole plugging when the V _ mean _ SP is larger than a third threshold value, and the hole plugging working condition is judged to be normal first and then hole plugging when the V _ mean _ SP is smaller than or equal to the third threshold value;

the second hole plugging working condition identification program comprises:

and (f) (t) calculating the average voltage V _ mean _ ON of the small hole voltage curve in a third preset time period after the start-up, judging that the small hole is blocked when the V _ mean _ ON is greater than a fourth threshold, and judging that the small hole is not blocked when the working condition of hole blocking is full-course hole blocking, and when the V _ mean _ ON is less than or equal to the fourth threshold.

2. The method of claim 1, wherein the derivative of the pair of pore voltage curves is obtained by deriving a derivative function thereofThe method also comprises the following steps:

calculating an average value HV _ mean of historical pinhole voltages according to a historical pinhole voltmeter, wherein the historical pinhole voltmeter comprises the voltage between electrodes on two sides of a pinhole when the hematology analyzer is started;

resetting all values of the small-hole voltage curve V-f (t) with the voltage lower than the average value HV _ mean to obtain a processed small-hole voltage curve V-F (t);

the derivative of the small-hole voltage curve is obtained to obtain a derivative function thereofAnd the small pore voltage curves in the subsequent steps are processed small pore voltage curves V ═ F (t);

the second plugging working condition identification program further includes: and when the small hole is judged to be not blocked, calculating the mean value V _ mean of the small hole voltage curve V ═ F (t), and storing the mean value V _ mean into the historical small hole voltage table.

3. The method of claim 2, wherein the first threshold is n times the standard deviation of the historical pinhole voltage, where n e (0,3 ].

4. The method of claim 2, wherein the second threshold is 3 times the standard deviation of the historical pinhole voltages.

5. The method of claim 2, wherein the third threshold is 3 times the standard deviation of the historical pinhole voltages.

6. The method of claim 2, wherein the fourth threshold is 3 times the standard deviation of the historical pinhole voltages.

7. The method of claim 1, further comprising: executing a failure mechanism when it is determined that the orifice is clogged, the failure mechanism comprising:

when the working condition of hole plugging is judged to be normal firstly and then hole plugging is judged, reporting abnormal information of a white/red blood cell channel, and issuing a plugging removal instruction; calculating an effective acquisition time length T1, counting white blood cells/red blood cells according to the number of pulses in the effective acquisition time length T1 when the effective acquisition time length T1 accounts for more than half of the total acquisition time length T0, and outputting a counting result; when the effective collection time period T1 is less than half of the total collection time period T0, the counting of white blood cells/red blood cells is not performed, and the counting result of white blood cells/red blood cells is masked in the output, wherein the calculation formula of the effective collection time period T1 is T1 ═ a [2k ];

when the working condition of hole plugging is judged to be that the hole plugging is performed firstly and then normal, reporting abnormal information of a white/red blood cell channel; calculating an effective acquisition time length T1, counting white blood cells/red blood cells according to the number of pulses in the effective acquisition time length T1 when the effective acquisition time length T1 accounts for more than half of the total acquisition time length T0, and outputting a counting result; when the effective collection time period T1 is less than half of the total collection time period T0, the white blood cells/red blood cells are not counted, and the white blood cells/red blood cells count result is masked in the output, wherein the calculation formula of the effective collection time period T1 is T1 — T0-a [2k +1 ];

and when the working condition of hole plugging is judged to be full-course hole plugging, reporting abnormal information of the white/red blood cell channel, issuing a plugging removal instruction, and shielding counting results of the white blood cells/red blood cells in output.

8. A small hole plugging recognition device of a blood cell analyzer is characterized by comprising:

the voltage data acquisition module is used for acquiring voltage between electrodes on two sides of the small hole within a period of time to obtain a small hole voltage curve V ═ f (t), wherein V is a voltage value, and t is time;

an inflection point obtaining module, configured to derive a derivative of the small-pore voltage curve V ═ f (t), so as to obtain a derivative function thereofAccording to derivative functionObtain the inflection point position A [ i ]]Wherein A [ i ]]Represents the position of the ith corner, i ∈ (1,2, ·, N);

an integral calculation module for calculating the integral of the derivative function between two adjacent inflection points, the calculation formula is

An integral absolute value comparison module for comparing all the integral values V_j(t) calculating an absolute value, taking the maximum value MAXV thereof, where MAXV ═ V, and comparing it with a first threshold value_k(t)|，k∈(1,2,…,N/2)；

A first hole-blocking condition identification module for judging that the small hole is blocked and the inflection point position A [2k ] is reached when the MAXV is greater than a first threshold value]The inflection point position A [2k +1] is the starting point of hole plugging]For the end point of the plugging hole, when V_k(t)<When 0, calculating the small hole voltage curve V ═ f (t) at the end point A [2k + 1-]The average voltage V _ mean _ EP in the first preset time period later is when the V _ mean _ EP is larger than a second threshold valueIf so, judging that the hole plugging working condition is full-course hole plugging, and when the V _ mean _ EP is less than or equal to a second threshold value, judging that the hole plugging working condition is normal after first hole plugging; when V is_k(t)>When 0, calculating the small hole voltage curve V ═ f (t) at the starting point A [2k ] of the hole plugging]The average voltage V _ mean _ SP in the second preset time period is judged to be full-course hole plugging when the V _ mean _ SP is larger than a third threshold value, and the hole plugging working condition is judged to be normal first and then hole plugging when the V _ mean _ SP is smaller than or equal to the third threshold value;

and the second plugging working condition identification module is used for calculating the average voltage V _ mean _ ON of the small hole voltage curve V ═ f (t) in a third preset time period after the starting-up when the MAXV is less than or equal to the first threshold, judging that the small hole is plugged when the V _ mean _ ON is greater than a fourth threshold, judging that the small hole is plugged in the whole plugging working condition, and judging that the small hole is not plugged when the V _ mean _ ON is less than or equal to the fourth threshold.

9. The apparatus of claim 8, further comprising a failure mechanism module to:

when the working condition of hole plugging is judged to be normal firstly and then hole plugging is judged, reporting abnormal information of a white/red blood cell channel, and issuing a plugging removal instruction; calculating an effective acquisition time length T1, counting white blood cells/red blood cells according to the number of pulses in the effective acquisition time length T1 when the effective acquisition time length T1 accounts for more than half of the total acquisition time length T0, and outputting a counting result; when the effective collection time period T1 is less than half of the total collection time period T0, the counting of white blood cells/red blood cells is not performed, and the counting result of white blood cells/red blood cells is masked in the output, wherein the calculation formula of the effective collection time period T1 is T1 ═ a [2k ];

when the working condition of hole plugging is judged to be that the hole plugging is performed firstly and then normal, reporting abnormal information of a white/red blood cell channel; calculating an effective acquisition time length T1, counting white blood cells/red blood cells according to the number of pulses in the effective acquisition time length T1 when the effective acquisition time length T1 accounts for more than half of the total acquisition time length T0, and outputting a counting result; when the effective collection time period T1 is less than half of the total collection time period T0, the white blood cells/red blood cells are not counted, and the white blood cells/red blood cells count result is masked in the output, wherein the calculation formula of the effective collection time period T1 is T1 — T0-a [2k +1 ];

and when the working condition of hole plugging is judged to be full-course hole plugging, reporting abnormal information of the white/red blood cell channel, issuing a plugging removal instruction, and shielding counting results of the white blood cells/red blood cells in output.

10. A computer-readable storage medium, characterized by comprising a program executable by a processor to implement the method of any one of claims 1 to 7.

Technical Field

The invention relates to the technical field of medical equipment, in particular to a method and a device for identifying small hole blockage of a hematology analyzer.

Background

The blood cell analyzer usually uses the coulter principle to count and group the blood cells. Referring to fig. 1, as shown in fig. 1, a small hole is formed in a detector of an analyzer, and a pair of positive and negative electrodes are formed on both sides of the small hole and connected to a constant current source. And uniformly mixing the blood sample in the diluent to obtain a blood cell diluent, wherein the small holes are soaked by the blood cell diluent. Because the cells are poor electric conductors, when blood cells in the diluted sample pass through the small holes under the action of pressure, the resistance between the electrodes changes, and thus a pulse signal proportional to the volume of the cells is formed at two ends of each electrode. The number of pulses corresponds to the number of cells passing through the aperture, and the amplitude of the pulses is proportional to the volume of cells passing through the aperture, thereby allowing detection of the number of cells and the size of the cells in the blood sample. Due to the possible existence of contaminants in the blood sample, contaminants may accumulate on the small hole, and the small hole may be blocked in the measurement process, so that the obtained pulse amplitude may be distorted, thereby affecting the accuracy and reliability of the detection result. It is therefore necessary to identify clogging of the pores of the analyzer.

In one prior art, whether a hole is blocked is determined by the stability of a particle flow, and if the particle flow jumps after a certain time, the hole is considered to be blocked. The method has the defects that the particle flow has no obvious change when the hole is plugged in the whole process, and the plugged hole cannot be accurately reported; when the sample is not sufficiently mixed, the stability of the particle flow cannot be ensured, and the hole is easily blocked by misinformation.

In the prior art, whether a hole is blocked is judged according to the change of a voltage curve between electrodes on two sides of the hole, and if the voltage between the electrodes on two sides of the hole has obvious change after a certain moment, the hole is considered to be blocked. The method has the defect that when the hole is plugged in the whole process, the voltage between the electrodes on two sides of the small hole may not be obviously changed, and the plugging hole cannot be accurately reported.

In the prior art, the voltage between electrodes on two sides of a small hole is taken as reference when starting up, whether the hole is blocked is judged by combining the change of a voltage curve between the electrodes on two sides of the small hole, and if the voltage between the electrodes on two sides of the small hole is unchanged but is obviously higher than the starting voltage, the whole-process hole blocking is judged; if the voltage between the electrodes on the two sides of the small hole has obvious change after a certain time, the hole is considered to be blocked. The method has the defects that when the temperature of the current diluent is obviously different from the temperature of the diluent during startup, the reference voltage during startup loses reference meaning due to different conductivities of the diluent at different temperatures, and the hole plugging cannot be accurately reported. For example, when the computer is started in the morning, the conductivity of the diluent is changed after the ambient temperature rises in the noon, and the reference voltage loses reference meaning when the computer is started in the morning.

Disclosure of Invention

The application provides a method and a device for identifying small hole plugging of a hematology analyzer, which can identify the hole plugging working condition of the small hole of the hematology analyzer more accurately.

According to a first aspect, an embodiment provides a small hole blockage identification method for a blood cell analyzer, comprising:

acquiring voltage between electrodes on two sides of a small hole within a period of time to obtain a small hole voltage curve V ═ f (t), wherein V is a voltage value, and t is time;

derivative the voltage curve V ═ f (t) of the small hole to obtain its derivative functionAccording to derivative functionObtain the inflection point position A [ i ]]Wherein A [ i ]]The position of the ith inflection point, i ∈ (1,2, ", N);

calculating the integral of a derivative function between two adjacent inflection points by the formula

For all integrated values V_j(t) calculating the absolute value, taking the maximum value MAXV, MAXV ═ V_k(t)|，k∈(1,2,…,N/2)；

Comparing the maximum value MAXV of the integral absolute value with a first threshold, when MAXV is larger than the first threshold, judging that the small hole is blocked, wherein the inflection point position A < 2k > is a hole blocking starting point, the inflection point position A < 2k + 1> is a hole blocking end point, and executing a first hole blocking working condition identification program; when the MAXV is smaller than or equal to the first threshold value, executing a second hole plugging working condition identification program;

the first hole plugging condition identification program comprises:

when V is_k(t)<When 0, calculating the small hole voltage curve V ═ f (t) at the end point A [2k + 1-]Average voltage in later first predetermined time periodV _ mean _ EP, when the V _ mean _ EP is larger than a second threshold value, judging that the hole plugging working condition is full-course hole plugging, and when the V _ mean _ EP is smaller than or equal to the second threshold value, judging that the hole plugging working condition is normal after first hole plugging;

when V is_k(t)>When 0, calculating the small hole voltage curve V ═ f (t) at the starting point A [2k ] of the hole plugging]The average voltage V _ mean _ SP in the second preset time period is judged to be full-course hole plugging when the V _ mean _ SP is larger than a third threshold value, and the hole plugging working condition is judged to be normal first and then hole plugging when the V _ mean _ SP is smaller than or equal to the third threshold value;

the second hole plugging working condition identification program comprises:

and (f) (t) calculating the average voltage V _ mean _ ON of the small hole voltage curve in a third preset time period after the start-up, judging that the small hole is blocked when the V _ mean _ ON is greater than a fourth threshold, and judging that the small hole is not blocked when the working condition of hole blocking is full-course hole blocking, and when the V _ mean _ ON is less than or equal to the fourth threshold.

In one embodiment, the derivative of the small-hole voltage curve V ═ f (t) is obtained to obtain a derivative function thereofThe method also comprises the following steps:

calculating an average value HV _ mean of historical pinhole voltages according to a historical pinhole voltmeter, wherein the historical pinhole voltmeter comprises the voltage between electrodes on two sides of a pinhole when the hematology analyzer is started;

resetting all values of the small-hole voltage curve V-f (t) with the voltage lower than the average value HV _ mean to obtain a processed small-hole voltage curve V-F (t);

the derivative of the small-hole voltage curve is obtained to obtain a derivative function thereofAnd the small pore voltage curves in the subsequent steps are processed small pore voltage curves V ═ F (t);

the second plugging working condition identification program further includes: and when the small hole is judged to be not blocked, calculating the mean value V _ mean of the small hole voltage curve V ═ F (t), and storing the mean value V _ mean into the historical small hole voltage table.

In one embodiment, the first threshold is n times the standard deviation of the historical pinhole voltage, where n ∈ (0, 3).

In one embodiment, the second threshold is 3 times the standard deviation of the historical pinhole voltage.

In one embodiment, the third threshold is 3 times the standard deviation of the historical pinhole voltage.

In one embodiment, the fourth threshold is 3 times the standard deviation of the historical pinhole voltage.

In one embodiment, the method further comprises: executing a failure mechanism when it is determined that the orifice is clogged, the failure mechanism comprising:

when the working condition of hole plugging is judged to be normal firstly and then hole plugging is judged, reporting abnormal information of a white/red blood cell channel, and issuing a plugging removal instruction; calculating an effective acquisition time length T1, counting white blood cells/red blood cells according to the number of pulses in the effective acquisition time length T1 when the effective acquisition time length T1 accounts for more than half of the total acquisition time length T0, and outputting a counting result; when the effective collection time period T1 is less than half of the total collection time period T0, the counting of white blood cells/red blood cells is not performed, and the counting result of white blood cells/red blood cells is masked in the output, wherein the calculation formula of the effective collection time period T1 is T1 ═ a [2k ];

when the working condition of hole plugging is judged to be that the hole plugging is performed firstly and then normal, reporting abnormal information of a white/red blood cell channel; calculating an effective acquisition time length T1, counting white blood cells/red blood cells according to the number of pulses in the effective acquisition time length T1 when the effective acquisition time length T1 accounts for more than half of the total acquisition time length T0, and outputting a counting result; when the effective collection time period T1 is less than half of the total collection time period T0, the white blood cells/red blood cells are not counted, and the white blood cells/red blood cells count result is masked in the output, wherein the calculation formula of the effective collection time period T1 is T1 — T0-a [2k +1 ];

and when the working condition of hole plugging is judged to be full-course hole plugging, reporting abnormal information of the white/red blood cell channel, issuing a plugging removal instruction, and shielding counting results of the white blood cells/red blood cells in output.

According to a second aspect, an embodiment provides a small hole clogging recognition apparatus for a blood cell analyzer, including:

the voltage data acquisition module is used for acquiring voltage between electrodes on two sides of the small hole within a period of time to obtain a small hole voltage curve V ═ f (t), wherein V is a voltage value, and t is time;

an inflection point obtaining module, configured to derive a derivative of the small-pore voltage curve V ═ f (t), so as to obtain a derivative function thereofAccording to derivative functionObtain the inflection point position A [ i ]]Wherein A [ i ]]The position of the ith inflection point, i ∈ (1,2, ", N);

an integral calculation module for calculating the integral of the derivative function between two adjacent inflection points, the calculation formula is

An integral absolute value comparison module for comparing all the integral values V_j(t) calculating an absolute value, taking the maximum value MAXV thereof, where MAXV ═ V, and comparing it with a first threshold value_k(t)|，k∈(1,2,…,N/2)；

A first hole-blocking condition identification module for judging that the small hole is blocked and the inflection point position A [2k ] is reached when the MAXV is greater than a first threshold value]The inflection point position A [2k +1] is the starting point of hole plugging]For the end point of the plugging hole, when V_k(t)<When 0, calculating the small hole voltage curve V ═ f (t) at the end point A [2k + 1-]Then average voltage V _ mean _ EP in a first preset time period, when the V _ mean _ EP is larger than a second threshold value, judging that the hole plugging working condition is full-course hole plugging, and when the V _ mean _ EP is smaller than or equal to the second threshold value, judging that the hole plugging working condition is normal after first hole plugging; when V is_k(t)>When 0, calculating the small hole voltage curve V ═ f (t) at the starting point A [2k ] of the hole plugging]And when the average voltage V _ mean _ SP in the second preset time period is greater than a third threshold value, judging that the hole plugging working condition is full hole plugging, and when the average voltage V _ mean _ SP is less than or equal to the third threshold value,judging the working condition of hole plugging is that the hole plugging is carried out after the hole plugging is normal;

and the second plugging working condition identification module is used for calculating the average voltage V _ mean _ ON of the small hole voltage curve V ═ f (t) in a third preset time period after the starting-up when the MAXV is less than or equal to the first threshold, judging that the small hole is plugged when the V _ mean _ ON is greater than a fourth threshold, judging that the small hole is plugged in the whole plugging working condition, and judging that the small hole is not plugged when the V _ mean _ ON is less than or equal to the fourth threshold.

In one embodiment, the apparatus further comprises a failure mechanism module to:

when the working condition of hole plugging is judged to be normal firstly and then hole plugging is judged, reporting abnormal information of a white/red blood cell channel, and issuing a plugging removal instruction; calculating an effective acquisition time length T1, counting white blood cells/red blood cells according to the number of pulses in the effective acquisition time length T1 when the effective acquisition time length T1 accounts for more than half of the total acquisition time length T0, and outputting a counting result; when the effective collection time period T1 is less than half of the total collection time period T0, the counting of white blood cells/red blood cells is not performed, and the counting result of white blood cells/red blood cells is masked in the output, wherein the calculation formula of the effective collection time period T1 is T1 ═ a [2k ];

when the working condition of hole plugging is judged to be that the hole plugging is performed firstly and then normal, reporting abnormal information of a white/red blood cell channel; calculating an effective acquisition time length T1, counting white blood cells/red blood cells according to the number of pulses in the effective acquisition time length T1 when the effective acquisition time length T1 accounts for more than half of the total acquisition time length T0, and outputting a counting result; when the effective collection time period T1 is less than half of the total collection time period T0, the white blood cells/red blood cells are not counted, and the white blood cells/red blood cells count result is masked in the output, wherein the calculation formula of the effective collection time period T1 is T1 — T0-a [2k +1 ];

and when the working condition of hole plugging is judged to be full-course hole plugging, reporting abnormal information of the white/red blood cell channel, issuing a plugging removal instruction, and shielding counting results of the white blood cells/red blood cells in output.

According to a third aspect, an embodiment provides a computer-readable storage medium comprising a program executable by a processor to implement the method for identifying a plugged pore in a small pore of a hematology analyzer of the first aspect.

According to the small hole plugging identification method and device of the blood cell analyzer and the computer-readable storage medium of the embodiment, the inflection point of the small hole voltage curve is obtained by deriving the small hole voltage curve, the integral of the derivative function between the inflection points is obtained to identify the plugging condition, the time point of occurrence or ending of plugging is obtained by combining the position of the inflection point, when the plugging is found, the plugging working condition can be further judged by combining the average voltage of the small hole voltage curve in a preset time period, the phenomena that the plugging is not reported and the plugging is mistakenly reported are avoided, and the plugging working condition of the small hole can be identified more accurately.

Drawings

FIG. 1 is a schematic diagram of a blood cell analysis according to the Coulter principle;

FIG. 2 is a graph of the pore voltage at normal conditions in one embodiment;

FIG. 3 is a schematic flow chart illustrating a method for identifying a plugged hole in a small hole of a blood cell analyzer according to an embodiment;

FIG. 4 is a schematic flow chart illustrating a first plugging condition identification procedure in accordance with an exemplary embodiment;

FIG. 5 is a schematic view of a full plugging of the orifice in one embodiment;

FIG. 6 is a schematic view of an embodiment in which the aperture is plugged first and then normal;

FIG. 7 is a schematic view of an alternative embodiment of the orifice;

FIG. 8 is a schematic view of an embodiment in which the aperture is normal and then plugged;

FIG. 9 is a flowchart illustrating a second plugging condition identification procedure according to an embodiment;

FIG. 10 is a schematic view of a full plugging of the orifice in yet another embodiment;

FIG. 11 is a schematic structural diagram of a small hole blockage recognition device of a blood cell analyzer according to an embodiment.

Detailed Description

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

The aperture voltage curve refers to the curve of the voltage between electrodes on both sides of the aperture of the hematology analyzer along with the time change, as shown in fig. 2, the curve is an approximately stable curve in the normal state, and has little large fluctuation, only when the aperture is blocked, the voltage of the aperture is obviously higher than that of the aperture in the normal state. The application provides a small hole plugging recognition method of a blood cell analyzer based on a small hole voltage curve.

Referring to fig. 3, as shown in fig. 3, an embodiment of a method for identifying a plugged hole includes steps S100 to S700, which are described in detail below.

Step S100: and acquiring the voltage between electrodes on two sides of the small hole within a period of time to obtain a small hole voltage curve V ═ f (t), wherein V is a voltage value, and t is time. Specifically, the aperture voltage curve may be obtained each time a sample is collected and analyzed.

Step S200: derivative the voltage curve V ═ f (t) of the small hole to obtain its derivative functionAccording to derivative functionObtain the inflection point position A [ i ]]Wherein A [ i ]]Denotes the location of the ith corner, i ∈ (1,2, ", N). The inflection point position refers to the time corresponding to the inflection point, and the Nth inflection point is the last inflection point.

Step S300: calculating the integral of a derivative function between two adjacent inflection points by the formula

Step S400: for all integrated values V_j(t) calculating the absolute value, taking the maximum value MAXV, i.e. MAXV ═ max (| V)_j(t) |). Suppose V when j is k_j(t) has a maximum absolute value, MAXV ═ V_k(t) |, where k ∈ (1,2, …, N/2). The maximum value of the integrated absolute value MAXV can be used to estimate the degree of hole plugging.

Step S500: comparing the maximum value MAXV of the integral absolute value with a preset first threshold, when MAXV is larger than the first threshold, judging that the small hole is blocked, marking the inflection point position A2 k as a hole blocking starting point as SP, and marking the inflection point position A2 k +1 as a hole blocking end point as EP, and executing a first hole blocking working condition identification program S600 to further identify the hole blocking working condition; when MAXV is less than or equal to the first threshold, the small hole may not be blocked, and the second hole blocking condition identifying program S700 is executed to further determine whether the hole is blocked.

Step S600: this step is a first hole plugging condition identification program, please refer to fig. 4, and the first hole plugging condition identification program specifically includes:

step S601: judgment V_k(t) if it is greater than 0, when V_k(t)<0, executing step S602, when V_k(t)>0, go to step S604.

Step S602: an average voltage V _ mean _ EP of the small-hole voltage curve V ═ f (t) in a first predetermined time period after the end point EP of plugging is calculated. At the moment, the working condition of the small hole can be judged to be firstly hole plugging, and the working condition of the hole plugging is further judged to be firstly hole plugging and then normal or whole-course hole plugging according to the average value of the voltage curve of the small hole after hole plugging.

The first predetermined time period may be the last period of time in the total acquisition time period T0, for example, the last 0.1 second, the last 0.5 second, or the last 1 second, and may also be an effective acquisition time period T1, where the effective acquisition time period T1 is the time from the hole-plugging end point EP to the end of the acquisition, that is, the effective acquisition time period T1 is T0-EP.

Step S603: comparing the V _ mean _ EP with a second threshold, and when the V _ mean _ EP is greater than the second threshold, determining that the hole plugging working condition is full-course hole plugging, as shown in fig. 5; when V _ mean _ EP is less than or equal to the second threshold, it is determined that the hole plugging condition is that the hole is plugged first and then normal, as shown in fig. 6.

Step S604: an average voltage V _ mean _ SP of the small-hole voltage curve V ═ f (t) in a second predetermined period of time before the plugging start point SP is calculated. The working condition of the small hole can be judged to be rear hole plugging, and whether the working condition of the hole plugging is normal first and then hole plugging or full hole plugging is further judged according to the average value of the voltage curve of the small hole before hole plugging.

The second predetermined time period may be the first period of time in the total acquisition time period T0, for example, the first 0.1 second, 0.5 second, or 1 second, and may also be the effective acquisition time period T1, where the effective acquisition time period T1 is the time from the start of acquisition to the hole blockage start point SP, that is, the effective acquisition time period T1 is SP.

Step S605: comparing the V _ mean _ SP with a third threshold, and when the V _ mean _ SP is greater than the third threshold, determining that the hole plugging working condition is full-course hole plugging, as shown in fig. 7; when V _ mean _ SP is less than or equal to the third threshold, it is determined that the hole plugging condition is normal first and then the hole plugging, as shown in fig. 8.

Step S700: this step is a second plugging condition identification program, please refer to fig. 9, and the second plugging condition identification program specifically includes:

step S701: and calculating the average voltage V _ mean _ ON of the small-hole voltage curve V ═ f (t) in a third preset time period after the startup. Wherein the third predetermined period of time may be selected as the first period of time in the total acquisition time period T0, such as the first 0.1, 0.5, or 1 second, etc.

Step S702: comparing the V _ mean _ ON with a fourth threshold, and when the V _ mean _ ON is greater than the fourth threshold, determining that the small hole is blocked, and the hole blocking working condition is full-course hole blocking, as shown in fig. 10; when V _ mean _ ON is less than or equal to the fourth threshold, it is determined that the orifice is not clogged, as shown in fig. 2.

In one embodiment, before deriving the pore voltage curve, an average value HV _ mean of the historical pore voltages is calculated according to a historical pore voltage table, all values of the pore voltage curve V ═ f (t) in which the voltage is lower than the average value HV _ mean are reset to HV _ mean, a processed pore voltage curve V ═ f (t) is obtained, the processed pore voltage curve V ═ f (t) is derived, and the processed pore voltage curve V ═ f (t) is also analyzed in the subsequent steps. The historical pinhole voltmeter comprises an initial voltage value between electrodes on two sides of a pinhole when the blood cell analyzer is started. In step S702 of the second plugging condition identification routine, when it is determined that the orifice is not plugged, a mean value V _ mean of the orifice voltage curve V ═ f (t) is calculated, and the mean value V _ mean is stored in the historical orifice voltage table. By using the average value of historical pore voltage to process the original pore voltage curve V (f) (t), the situation that the pore voltage of a certain period of time becomes low and the pore voltage curve fluctuates abnormally to influence the accuracy of pore blocking identification due to the fact that the needle point is immersed in the diluent of the hematology analyzer at a certain time because the height of the sampling needle of some instruments is not well adjusted can be avoided. Meanwhile, the average voltage of the pore voltage curve which is normally acquired every time is stored in a historical pore voltmeter, so that possible voltage distortion caused by only using the pore voltage during starting as the reference voltage is avoided, and more accurate reference voltage can be provided for pore blockage identification.

The standard deviation HV _ SD and the coefficient of variation HV _ CV of the historical pinhole voltage can also be calculated according to the historical pinhole voltage table. In one embodiment, the first threshold may be n times the standard deviation of the historical cell voltage HV _ SD, where n ∈ (0,3 ]. the second threshold may be 3 times the standard deviation of the historical cell voltage.A third threshold may be 3 times the standard deviation of the historical cell voltage.A fourth threshold may be 3 times the standard deviation of the historical cell voltage.A third threshold may be 99% of the normal distribution statistics, which may be used to measure the degree of deviation of the cell voltage from the reference voltage.

In one embodiment, the method for identifying the plugging of the small hole further comprises a failure mechanism, and the failure mechanism is executed when the small hole is judged to be plugged, and the failure mechanism comprises:

when the working condition of hole plugging is judged to be normal firstly and then hole plugging is judged, reporting abnormal information of a white/red blood cell channel, and issuing a plugging removal instruction; calculating an effective acquisition time length T1, counting white blood cells/red blood cells according to the number of pulses in the effective acquisition time length T1 when the effective acquisition time length T1 accounts for more than half of the total acquisition time length T0, namely T1> (T0/2), and outputting a counting result; when the effective collection time period T1 is less than half of the total collection time period T0, i.e., T1 ≦ (T0/2), the white blood cell/red blood cell count is not performed, and the white blood cell/red blood cell count result is masked in the output, where the calculation formula of the effective collection time period T1 is T1 ═ SP.

When the working condition of hole plugging is judged to be that the hole plugging is performed firstly and then normal, reporting abnormal information of a white/red blood cell channel; calculating an effective acquisition time length T1, counting white blood cells/red blood cells according to the number of pulses in the effective acquisition time length T1 when the effective acquisition time length T1 accounts for more than half of the total acquisition time length T0, namely T1> (T0/2), and outputting a counting result; when the effective collection time period T1 is less than half of the total collection time period T0, i.e., T1 ≦ (T0/2), the white blood cell/red blood cell count is not performed, and the white blood cell/red blood cell count result is masked in the output, wherein the calculation formula of the effective collection time period T1 is T1 — T0-EP.

And when the working condition of hole plugging is judged to be full-course hole plugging, reporting abnormal information of the white/red blood cell channel, issuing a plugging removal instruction, and shielding counting results of the white blood cells/red blood cells in output.

According to the hole plugging identification method, after the hole plugging working condition of a small hole is identified, the working condition which is not full-course hole plugging is analyzed, result shielding judgment is carried out according to effective acquisition time, when the effective acquisition time occupies more than half of the total acquisition time, the detection result can be utilized, the data of a normal section in the detection result is taken for calculation, and the result is reported, so that a user does not need to carry out retesting due to hole plugging; and a blockage removing instruction cannot be issued under the normal working condition after the hole is blocked, so that the test time is saved.

Referring to fig. 11, as shown in fig. 11, the small hole blockage recognition device in one embodiment includes a voltage data acquisition module 111, an inflection point acquisition module 112, an integral calculation module 113, an integral absolute value comparison module 114, a first hole blockage condition recognition module 115, and a second hole blockage condition recognition module 116, which are specifically described below.

The voltage data obtaining module 111 is configured to obtain a voltage between electrodes on two sides of the small hole within a period of time, and obtain a small hole voltage curve V ═ f (t), where V is a voltage value and t is time. Specifically, the aperture voltage curve may be obtained each time a sample is collected and analyzed.

The inflection point obtaining module 112 is configured to derive a derivative of the small-pore voltage curve V ═ f (t), so as to obtain a derivative function thereofAccording to derivative functionObtain the inflection point position A [ i ]]Wherein A [ i ]]Denotes the location of the ith corner, i ∈ (1,2, ", N). The inflection point position refers to the time corresponding to the inflection point, and the Nth inflection point is the last inflection point.

The integral calculation module 113 is used for calculating the integral of the derivative function between two adjacent inflection points, and the calculation formula is

The integrated absolute value comparison module 114 is used for comparing all the integrated values V_j(t) calculating an absolute value, taking the maximum value MAXV thereof, and comparing it with a first threshold, assuming that V is equal to k when j is equal to k_j(t) has a maximum absolute value, MAXV ═ V_k(t) |, k ∈ (1,2, …, N/2). The maximum value of the integrated absolute value MAXV can be used to estimate the degree of hole plugging.

The first plugging condition identification module 115 is configured to determine that the small hole is plugged and the inflection point position A [2k ] is reached when the MAXV is greater than a first threshold]Is the starting point of hole blocking, marked as SP, and the inflection point position A [2k +1]]The end point of plugging is marked as EP. When V is_k(t)<When the voltage of the small hole is 0, calculating an average voltage V _ mean _ EP of a small hole voltage curve V ═ f (t) in a first preset time period after a hole plugging end point EP, judging that the hole plugging working condition is full-course hole plugging when the V _ mean _ EP is greater than a second threshold, and judging that the hole plugging working condition is normal after first hole plugging when the V _ mean _ EP is less than or equal to the second threshold; when V is_k(t)>And when the voltage curve V is equal to f (t), calculating the average voltage V _ mean _ SP of the small hole voltage curve in a second preset time period before the hole plugging starting point SP, judging that the hole plugging working condition is full hole plugging when the V _ mean _ SP is greater than a third threshold, and judging that the hole plugging working condition is normal first and then hole plugging when the V _ mean _ SP is less than or equal to the third threshold.

The first predetermined time period may be the last period of time in the total acquisition time period T0, for example, the last 0.1 second, the last 0.5 second, or the last 1 second, and may also be an effective acquisition time period T1, where the effective acquisition time period T1 is the time from the hole plugging end point EP to the end of acquisition, that is, the effective acquisition time period T1 is T0-EP; the second predetermined time period may be the first period of time in the total acquisition time period T0, for example, the first 0.1 second, 0.5 second, or 1 second, and may also be the effective acquisition time period T1, where the effective acquisition time period T1 is the time from the start of acquisition to the hole blockage start point SP, that is, the effective acquisition time period T1 is SP.

The second plugging condition identification module 116 is configured to calculate an average voltage V _ mean _ ON of the small hole voltage curve V ═ f (t) in a third predetermined time period after the start-up, when MAXV is less than or equal to the first threshold, determine that the small hole is plugged when V _ mean _ ON is greater than a fourth threshold, and determine that the small hole is plugged in the whole plugging process, and determine that the small hole is not plugged when V _ mean _ ON is less than or equal to the fourth threshold. Wherein the third predetermined period of time may be selected as the first period of time in the total acquisition time period T0, such as the first 0.1, 0.5, or 1 second, etc.

In one embodiment, the inflection point obtaining module 112 further calculates an average value HV _ mean of the historical pore voltage according to a historical pore voltage table before deriving the pore voltage curve, resets all values of the pore voltage curve V ═ f (t) where the voltage is lower than the average value HV _ mean to HV _ mean, obtains a processed pore voltage curve V ═ f (t), and derives the processed pore voltage curve V ═ f (t). In other modules, the processed pore voltage curve V ═ f (t) is also analyzed. The historical pinhole voltmeter comprises an initial voltage value between electrodes on two sides of a pinhole when the blood cell analyzer is started. The second plugging condition identification module 116 is further configured to, when it is determined that the small hole is not plugged, calculate a mean value V _ mean of the small hole voltage curve V ═ f (t), and store the mean value V _ mean in the historical small hole voltage table. By using the average value of historical pore voltage to process the original pore voltage curve V (f) (t), the situation that the pore voltage of a certain period of time becomes low and the pore voltage curve fluctuates abnormally to influence the accuracy of pore blocking identification due to the fact that the needle point is immersed in the diluent of the hematology analyzer at a certain time because the height of the sampling needle of some instruments is not well adjusted can be avoided. Meanwhile, the average voltage of the pore voltage curve which is normally acquired every time is stored in a historical pore voltmeter, so that possible voltage distortion caused by only using the pore voltage during starting as the reference voltage is avoided, and more accurate reference voltage can be provided for pore blockage identification.

The standard deviation HV _ SD and the coefficient of variation HV _ CV of the historical pinhole voltage can also be calculated according to the historical pinhole voltage table. In one embodiment, the first threshold may be n times the standard deviation of the historical cell voltage HV _ SD, where n ∈ (0,3 ]. the second threshold may be 3 times the standard deviation of the historical cell voltage.A third threshold may be 3 times the standard deviation of the historical cell voltage.A fourth threshold may be 3 times the standard deviation of the historical cell voltage.A third threshold may be 99% of the normal distribution statistics, which may be used to measure the degree of deviation of the cell voltage from the reference voltage.

In one embodiment, the apparatus for identifying small hole plugging further comprises a failure mechanism module 117, wherein the failure mechanism module 117 is configured to:

when the working condition of hole plugging is judged to be normal firstly and then hole plugging is judged, reporting abnormal information of a white/red blood cell channel, and issuing a plugging removal instruction; calculating an effective acquisition time length T1, counting white blood cells/red blood cells according to the number of pulses in the effective acquisition time length T1 when the effective acquisition time length T1 accounts for more than half of the total acquisition time length T0, namely T1> (T0/2), and outputting a counting result; when the effective collection time period T1 is less than half of the total collection time period T0, i.e., T1 ≦ (T0/2), the white blood cell/red blood cell count is not performed, and the white blood cell/red blood cell count result is masked in the output, where the calculation formula of the effective collection time period T1 is T1 ═ SP.

When the working condition of hole plugging is judged to be that the hole plugging is performed firstly and then normal, reporting abnormal information of a white/red blood cell channel; calculating an effective acquisition time length T1, counting white blood cells/red blood cells according to the number of pulses in the effective acquisition time length T1 when the effective acquisition time length T1 accounts for more than half of the total acquisition time length T0, namely T1> (T0/2), and outputting a counting result; when the effective collection time period T1 is less than half of the total collection time period T0, i.e., T1 ≦ (T0/2), the white blood cell/red blood cell count is not performed, and the white blood cell/red blood cell count result is masked in the output, wherein the calculation formula of the effective collection time period T1 is T1 — T0-EP.

And when the working condition of hole plugging is judged to be full-course hole plugging, reporting abnormal information of the white/red blood cell channel, issuing a plugging removal instruction, and shielding counting results of the white blood cells/red blood cells in output.

The application discloses blood cell analyzer's aperture plugged hole identification method and device, derive through the aperture voltage curve, acquire the flex point of aperture voltage curve, and ask the integral of derivative function between the flex point to discern the plugged hole situation, combine the flex point position to obtain the time point that the plugged hole takes place or finishes, still can combine the average voltage of aperture voltage curve in the predetermined period of time to further judge the plugged hole operating mode when discovering the plugged hole, avoided the plugged hole not report and the plugged hole of wrong report, thereby can discern the plugged hole operating mode of aperture more accurately. Meanwhile, after the working condition of hole plugging of the small hole is identified, the working condition which is not full-course hole plugging is analyzed, result shielding judgment is carried out according to effective acquisition time, when the effective acquisition time accounts for more than half of the total acquisition time, the detection result can be utilized, the data of a normal section in the detection result is taken for calculation, and the result is reported, so that a user does not need to carry out retesting due to hole plugging; and a blockage removing instruction cannot be issued under the normal working condition after the hole is blocked, so that the test time is saved.

Reference is made herein to various exemplary embodiments. However, those skilled in the art will recognize that changes and modifications may be made to the exemplary embodiments without departing from the scope hereof. For example, the various operational steps, as well as the components used to perform the operational steps, may be implemented in differing ways depending upon the particular application or consideration of any number of cost functions associated with operation of the system (e.g., one or more steps may be deleted, modified or incorporated into other steps).

In the above embodiments, the implementation may be wholly or partially realized by software, hardware, firmware, or any combination thereof. Additionally, as will be appreciated by one skilled in the art, the principles herein may be reflected in a computer program product on a computer readable storage medium, which is pre-loaded with computer readable program code. Any tangible, non-transitory computer-readable storage medium may be used, including magnetic storage devices (hard disks, floppy disks, etc.), optical storage devices (CD-to-ROM, DVD, Blu-Ray discs, etc.), flash memory, and/or the like. These computer program instructions may be loaded onto a general purpose computer, special purpose computer, or other programmable data processing apparatus to produce a machine, such that the instructions which execute on the computer or other programmable data processing apparatus create means for implementing the functions specified. These computer program instructions may also be stored in a computer-readable memory that can direct a computer or other programmable data processing apparatus to function in a particular manner, such that the instructions stored in the computer-readable memory produce an article of manufacture including means for implementing the function specified. The computer program instructions may also be loaded onto a computer or other programmable data processing apparatus to cause a series of operational steps to be performed on the computer or other programmable apparatus to produce a computer implemented process such that the instructions which execute on the computer or other programmable apparatus provide steps for implementing the functions specified.

While the principles herein have been illustrated in various embodiments, many modifications of structure, arrangement, proportions, elements, materials, and components particularly adapted to specific environments and operative requirements may be employed without departing from the principles and scope of the present disclosure. The above modifications and other changes or modifications are intended to be included within the scope of this document.

The foregoing detailed description has been described with reference to various embodiments. However, one skilled in the art will recognize that various modifications and changes may be made without departing from the scope of the present disclosure. Accordingly, the disclosure is to be considered in an illustrative and not a restrictive sense, and all such modifications are intended to be included within the scope thereof. Also, advantages, other advantages, and solutions to problems have been described above with regard to various embodiments. However, the benefits, advantages, solutions to problems, and any element(s) that may cause any element(s) to occur or become more pronounced are not to be construed as a critical, required, or essential feature or element of any or all the claims. As used herein, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, system, article, or apparatus. Furthermore, the term "coupled," and any other variation thereof, as used herein, refers to a physical connection, an electrical connection, a magnetic connection, an optical connection, a communicative connection, a functional connection, and/or any other connection.

Those skilled in the art will recognize that many changes may be made to the details of the above-described embodiments without departing from the underlying principles of the invention. Accordingly, the scope of the invention should be determined only by the claims.