阅读说明：本技术 基于血液细胞分析仪的特定蛋白分析方法和控制装置 (Specific protein analysis method and control device based on blood cell analyzer ) 是由 王兴红 邹海涛 于 2020-11-23 设计创作，主要内容包括：本发明公开了一种基于血液细胞分析仪的特定蛋白分析方法和控制装置,所述方法包括：在待测样本注入反应杯进行特定蛋白分析过程中,获取反应杯中样本液的反应曲线,基于反应曲线可获取反应曲线的全程绝对面积和全程相对面积,由于在特定蛋白分析过程中蛋白会附着在反应杯上,导致反应杯对应的电信号参数整体增大,即反应曲线整体抬高,从而使得全程绝对面积变大,全程相对面积变小,因此通过获取待测样本分析后的全程绝对面积和全程相对面积判断是否需要对反应杯进行清洗,这样无需每次对待测样本分析完成后都对反应杯进行清洗,减少了清洗次数,节省了样本分析时间。(The invention discloses a specific protein analysis method and a control device based on a blood cell analyzer, wherein the method comprises the following steps: the reaction curve of the sample liquid in the reaction cup is obtained in the process of injecting a sample to be detected into the reaction cup to perform specific protein analysis, the whole-course absolute area and the whole-course relative area of the reaction curve can be obtained based on the reaction curve, and the protein can be attached to the reaction cup in the specific protein analysis process, so that the electric signal parameters corresponding to the reaction cup are integrally increased, namely the reaction curve is integrally raised, the whole-course absolute area is increased, and the whole-course relative area is reduced.)

1. A specific protein analysis method based on a blood cell analyzer, wherein the blood cell analyzer includes a cuvette for performing specific protein analysis on a sample to be tested and a cleaning device for cleaning the cuvette, the method comprising:

injecting the sample to be tested into the reaction cup to perform at least one specific protein analysis, and simultaneously obtaining a reaction curve of a sample liquid in the reaction cup, wherein the reaction curve is used for representing the corresponding relation between the electric signal parameters corresponding to the sample liquid and the specific protein analysis time in the specific protein analysis process;

determining the whole-process absolute area and the whole-process relative area of the reaction curve according to the reaction curve, wherein the whole-process absolute area is used for representing the areas of the reaction curve, the electric signal parameter axis and the region surrounded by the specific protein analysis time axis, and the whole-process relative area is used for representing the areas of the reaction curve, the electric signal parameter value range in the reaction curve and the region surrounded by the specific protein analysis time axis;

acquiring a first calibration curve and a second calibration curve, wherein the first calibration curve is used for representing the corresponding relation between the whole absolute area and the concentration of the sample liquid, and the second calibration curve is used for representing the corresponding relation between the whole relative area and the concentration of the sample liquid;

determining a first concentration of the sample liquid corresponding to the whole absolute area based on a first calibration curve; determining a second concentration of the sample liquid corresponding to the whole-course relative area based on a second calibration curve;

and sending a reaction cup cleaning triggering instruction to the cleaning device according to the magnitude relation between the first concentration and the second concentration, and cleaning the reaction cup.

2. The method of claim 1, wherein said obtaining a first scaling curve and a second scaling curve comprises:

obtaining a plurality of standard samples with known concentrations, wherein the plurality of standard samples have different concentrations;

sequentially injecting a plurality of standard samples into the reaction cup for analyzing at least one specific protein to obtain a plurality of standard reaction curves;

acquiring a global absolute area and a global relative area in a plurality of standard reaction curves;

fitting according to the concentrations of the plurality of standard samples and the plurality of global absolute areas to obtain a first calibration curve; and fitting to obtain a second calibration curve according to the concentrations of the plurality of standard samples and the plurality of global relative areas.

3. The method of claim 2, wherein said sequentially injecting a plurality of said standard samples into said reaction cuvette for at least one specific protein analysis comprises:

injecting a plurality of standard samples into the reaction cup for at least one specific protein analysis in the following way:

injecting a standard sample into the reaction cup for at least one specific protein analysis; and after the analysis is finished, sending a reaction cup cleaning triggering instruction to the cleaning device to clean the reaction cup.

4. The method of claim 1, wherein sending a cuvette washing trigger instruction to the washing device according to the magnitude relation between the first concentration and the second concentration comprises:

if the ratio of the second concentration to the first concentration is smaller than or equal to a preset threshold value, sending a reaction cup cleaning trigger instruction to the cleaning device;

or if the ratio of the first concentration to the second concentration is greater than or equal to a preset threshold value, sending a reaction cup cleaning triggering instruction to the cleaning device.

5. The method of claim 1, wherein the electrical signal parameter is a voltage value.

6. A specific protein analysis control apparatus based on a blood cell analyzer, wherein the blood cell analyzer includes a cuvette for performing specific protein analysis on a sample to be tested and a cleaning device for cleaning the cuvette, the control apparatus comprising:

the specific protein analysis unit is used for injecting the sample to be tested into the reaction cup to perform at least one specific protein analysis and simultaneously acquiring a reaction curve of a sample liquid in the reaction cup, wherein the reaction curve is used for representing the corresponding relation between an electric signal parameter corresponding to the sample liquid and specific protein analysis time in the specific protein analysis process;

the area acquisition unit is used for determining the whole-process absolute area and the whole-process relative area of the reaction curve according to the reaction curve, wherein the whole-process absolute area is used for representing the areas of the reaction curve, the electric signal parameter axis and the region surrounded by the specific protein analysis time axis, and the whole-process relative area is used for representing the areas of the reaction curve, the electric signal parameter value range in the reaction curve and the region surrounded by the specific protein analysis time axis;

the calibration curve acquisition unit is used for acquiring a first calibration curve and a second calibration curve, wherein the first calibration curve is used for representing the corresponding relation between the whole absolute area and the concentration of the sample liquid, and the second calibration curve is used for representing the corresponding relation between the whole relative area and the concentration of the sample liquid;

the concentration determining unit is used for determining the first concentration of the sample liquid corresponding to the whole absolute area based on a first calibration curve; determining a second concentration of the sample liquid corresponding to the whole-course relative area based on a second calibration curve;

and the cleaning triggering unit is used for sending a reaction cup cleaning triggering instruction to the cleaning device according to the magnitude relation between the first concentration and the second concentration so as to clean the reaction cup.

7. The control apparatus of claim 6, wherein said obtaining the first and second scaling curves comprises:

obtaining a plurality of standard samples with known concentrations, wherein the plurality of standard samples have different concentrations;

sequentially injecting a plurality of standard samples into the reaction cup for analyzing at least one specific protein to obtain a plurality of standard reaction curves;

acquiring a global absolute area and a global relative area in a plurality of standard reaction curves;

fitting according to the concentrations of the plurality of standard samples and the plurality of global absolute areas to obtain a first calibration curve; fitting according to the concentrations of the plurality of standard samples and the plurality of global relative areas to obtain a second calibration curve;

the step of sequentially injecting a plurality of standard samples into the reaction cup for at least one specific protein analysis comprises the following steps:

injecting a plurality of standard samples into the reaction cup for at least one specific protein analysis in the following way:

injecting a standard sample into the reaction cup for at least one specific protein analysis; and after the analysis is finished, sending a reaction cup cleaning triggering instruction to the cleaning device to clean the reaction cup.

8. The control device of claim 6, wherein the sending of a cuvette washing trigger instruction to the washing device according to the magnitude relation between the first concentration and the second concentration comprises:

if the ratio of the second concentration to the first concentration is smaller than or equal to a preset threshold value, sending a reaction cup cleaning trigger instruction to the cleaning device;

or if the ratio of the first concentration to the second concentration is greater than or equal to a preset threshold value, sending a reaction cup cleaning triggering instruction to the cleaning device.

9. The control device of claim 6, wherein the electrical signal parameter is a voltage value.

10. A computer-readable storage medium, characterized by comprising a program executable by a processor to implement the method of any one of claims 1-5.

Technical Field

The invention relates to the technical field of blood cell analyzers, in particular to a specific protein analysis method and a control device based on a blood cell analyzer.

Background

Blood cell analyzers are used for various analyses of blood components, such as technical and differential detection of white blood cells in blood, detection of hemoglobin concentration in red blood cells, and analytical detection of specific proteins in blood. The specific protein analysis is mainly to detect specific functional proteins in blood, such as immunoglobulin, etc., to understand various properties of the body.

When the blood cell analyzer analyzes specific protein, a tested blood sample is injected into the reaction cup, then different reagents are injected to analyze different specific proteins in the tested blood sample, after the analysis is finished, reaction liquid in the reaction cup is discharged from the liquid discharge port, diluent is injected into the reaction cup to wait for the analysis of the next tested blood sample, and when the tested blood sample is not injected into the reaction cup, the diluent is required to be injected into the reaction cup to prevent external air from entering the reaction cup. In order to prevent the reaction solution of the previous blood sample from remaining in the reaction cup and interfering with the analysis of the subsequent blood sample, the reaction cup needs to be cleaned.

The existing blood cell analyzer generally adopts a mode that a cleaning command is triggered to clean the reaction cup after the specific protein analysis is completed on one tested blood sample every time so as to clean the reaction cup. Thus, the number of washing times is large, the washing liquid for washing is excessively consumed, the detection cost is increased, and since the washing takes a long time, the time for detecting a specific protein in a blood sample at a time is prolonged.

Disclosure of Invention

The invention mainly solves the technical problems of reducing the cleaning times of the reaction cup and saving the sample analysis time.

According to a first aspect, an embodiment provides a specific protein analysis method based on a blood cell analyzer, the blood cell analyzer including a cuvette for performing specific protein analysis on a sample to be tested and a cleaning device for cleaning the cuvette, the method including:

injecting the sample to be tested into the reaction cup to perform at least one specific protein analysis, and simultaneously obtaining a reaction curve of a sample liquid in the reaction cup, wherein the reaction curve is used for representing the corresponding relation between the electric signal parameters corresponding to the sample liquid and the specific protein analysis time in the specific protein analysis process;

determining the whole-process absolute area and the whole-process relative area of the reaction curve according to the reaction curve, wherein the whole-process absolute area is used for representing the areas of the reaction curve, the electric signal parameter axis and the region surrounded by the specific protein analysis time axis, and the whole-process relative area is used for representing the areas of the reaction curve, the electric signal parameter value range in the reaction curve and the region surrounded by the specific protein analysis time axis;

acquiring a first calibration curve and a second calibration curve, wherein the first calibration curve is used for representing the corresponding relation between the whole absolute area and the concentration of the sample liquid, and the second calibration curve is used for representing the corresponding relation between the whole relative area and the concentration of the sample liquid;

determining a first concentration of the sample liquid corresponding to the whole absolute area based on a first calibration curve; determining a second concentration of the sample liquid corresponding to the whole-course relative area based on a second calibration curve;

and sending a reaction cup cleaning triggering instruction to the cleaning device according to the magnitude relation between the first concentration and the second concentration, and cleaning the reaction cup.

According to a second aspect, an embodiment provides a specific protein analysis control apparatus based on a blood cell analyzer including a cuvette for performing specific protein analysis on a sample to be tested and a cleaning device for cleaning the cuvette, the control apparatus including:

the specific protein analysis unit is used for injecting the sample to be tested into the reaction cup to perform at least one specific protein analysis and simultaneously acquiring a reaction curve of a sample liquid in the reaction cup, wherein the reaction curve is used for representing the corresponding relation between an electric signal parameter corresponding to the sample liquid and specific protein analysis time in the specific protein analysis process;

the area acquisition unit is used for determining the whole-process absolute area and the whole-process relative area of the reaction curve according to the reaction curve, wherein the whole-process absolute area is used for representing the areas of the reaction curve, the electric signal parameter axis and the region surrounded by the specific protein analysis time axis, and the whole-process relative area is used for representing the areas of the reaction curve, the electric signal parameter value range in the reaction curve and the region surrounded by the specific protein analysis time axis;

the calibration curve acquisition unit is used for acquiring a first calibration curve and a second calibration curve, wherein the first calibration curve is used for representing the corresponding relation between the whole absolute area and the concentration of the sample liquid, and the second calibration curve is used for representing the corresponding relation between the whole relative area and the concentration of the sample liquid;

the concentration determining unit is used for determining the first concentration of the sample liquid corresponding to the whole absolute area based on a first calibration curve; determining a second concentration of the sample liquid corresponding to the whole-course relative area based on a second calibration curve;

and the cleaning triggering unit is used for sending a reaction cup cleaning triggering instruction to the cleaning device according to the magnitude relation between the first concentration and the second concentration so as to clean the reaction cup.

According to a third aspect, an embodiment provides a computer-readable storage medium comprising a program executable by a processor to implement the method of the above-described embodiment.

According to the specific protein analysis method and the control device based on the blood cell analyzer of the embodiment, because the protein can be attached to the reaction cup in the specific protein analysis process, the electric signal parameters corresponding to the reaction cup are integrally increased, namely, the reaction curve is integrally raised, so that the whole-process absolute area is increased, the whole-process relative area is decreased, whether the reaction cup needs to be cleaned or not is judged by obtaining the whole-process absolute area and the whole-process relative area corresponding to the sample to be detected after the analysis is completed, the reaction cup does not need to be cleaned every time after the sample to be detected is analyzed, the cleaning times are reduced, and the sample analysis time is saved.

Drawings

FIG. 1 is a block diagram showing the structure of a specific protein analysis channel in a blood cell analyzer;

FIG. 2 is a flow chart of a specific protein analysis method based on a blood cell analyzer according to an embodiment;

FIG. 3 is a schematic diagram of a reaction curve of an embodiment;

FIG. 4 is a graphical representation of the absolute area over time and the relative area over time of the reaction curve for one embodiment;

FIG. 5 is a flowchart of a method of obtaining a first calibration curve and a second calibration curve according to an embodiment;

FIG. 6 is a schematic structural view of a specific protein analysis control apparatus according to an embodiment;

FIG. 7 is a graph showing the reaction curves of the same sample in the case where the cuvette is normal and the cuvette has protein attached.

Detailed Description

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

Some proteins are attached to the wall of the reaction cup during the analysis of specific proteins, and specific reagents are needed to clean the reaction cup, but if the reaction cup is cleaned once every sample is analyzed, the analysis cost is increased, and the whole analysis time is prolonged. In addition, protein attached to the wall of the cuvette will affect the refraction of light, please refer to fig. 7, fig. 7 is a schematic diagram of the reaction curve of the same sample when the cuvette is normal and the cuvette has protein attached, and once the protein is attached seriously, the accuracy of the specific protein analysis will be affected.

In the embodiment of the invention, the reaction curve of the sample liquid in the reaction cup is obtained in the process of injecting the sample to be tested into the reaction cup to carry out the specific protein analysis, the whole absolute area and the whole relative area of the reaction curve can be obtained based on the reaction curve, and the whole electric signal parameters corresponding to the reaction cup are integrally increased due to the fact that the protein is attached to the reaction cup in the specific protein analysis process, namely the reaction curve is integrally lifted, so that the whole absolute area is increased, and the whole relative area is reduced.

The blood cell analyzer of the embodiment of the present invention includes a specific protein analysis channel for performing at least one function of specific protein analysis on a blood sample to be tested, referring to fig. 1, the specific protein analysis channel includes a reaction cup 20 for performing specific protein analysis on the blood sample to be tested, a cleaning device 30 for cleaning the reaction cup, a diluent injection device 40 for injecting a diluent into the reaction cup 20, a sample injection device 50 for injecting the blood sample to be tested, a reagent injection device 60 for injecting a specific protein analysis reagent, a drain device 70 for draining the liquid in the reaction cup 20, and a control device 10 for controlling the above-mentioned cleaning device 30, diluent injection device 40, sample injection device 50, reagent injection device 60, and drain device 70, the control device 10 is used for controlling the analysis and detection of specific protein, referring to fig. 2, fig. 2 is a flowchart illustrating a specific protein analysis method based on a blood cell analyzer according to an embodiment, the method includes steps 101 to 104, which are described in detail below.

Step 101, injecting a sample to be tested into a reaction cup 20 for at least one specific protein analysis, and simultaneously obtaining a reaction curve V ═ f (T) of a sample liquid in the reaction cup 20, wherein V represents an electrical signal parameter, T represents time for the specific protein analysis, T is greater than or equal to 0 and less than or equal to T, and T represents total time for the specific protein analysis, and the reaction curve V ═ f (T) is used for representing a corresponding relation between the electrical signal parameter corresponding to the sample liquid and the time for the specific protein analysis in the process of analyzing the specific protein.

In this embodiment, when a specific protein analysis is required for a sample to be tested, the testing personnel performs related operations on the blood cell analyzer, so that the control device receives a specific protein analysis instruction input by the testing personnel. After receiving the specific protein analysis instruction, the control device 10 controls the sample to be detected to be injected into the reaction cup 20 through the sample injection device 50, after the sample to be detected is injected into the reaction cup 20, in the process that the sample to be detected performs the specific protein analysis in the reaction cup, the control device 10 obtains a reaction curve of the electrical signal parameter represented by the reaction cup along with the specific protein analysis time in real time, where the starting time of the reaction curve is the time when the sample to be detected starts the specific protein analysis, and the ending time of the reaction curve is the time when the sample to be detected finishes the specific protein analysis, please refer to fig. 3, where fig. 3 is a schematic diagram of the reaction curve of an embodiment, where a vertical coordinate is an electrical signal parameter axis and an abscissa is a time axis. The electrical signal parameter in this embodiment may be a voltage value, which can be obtained by an existing detection device in the blood cell analyzer, and as can be seen from fig. 3, as the analysis time of the specific protein increases, the magnitude of the electrical signal parameter also increases.

Step 102, determining a whole-course absolute area and a whole-course relative area of a reaction curve according to the reaction curve, please refer to fig. 4, where fig. 4 is a schematic diagram of the whole-course absolute area and the whole-course relative area of the reaction curve of an embodiment, where the whole-course absolute area R is used to characterize the reaction curve V ═ f (t), the area of the region surrounded by the electric signal parameter axis and the specific protein analysis time axis, and the whole-course relative area S is used to characterize the reaction curve V ═ f (t), the range of the electric signal parameter in the reaction curve and the area of the region surrounded by the specific protein analysis time axis, in this embodiment, the whole-course absolute area R is S + a, and a is the minimum value of the electric signal parameter in the reaction curve and the area of the region surrounded by the.

And 103, acquiring a first calibration curve r ═ f (c) and a second calibration curve s ═ g (c), wherein r represents the whole-process absolute area, c represents the concentration of the sample liquid in the reaction cup, and s represents the whole-process relative area, the first calibration curve r ═ f (c) is used for representing the corresponding relation between the whole-process absolute area and the concentration of the sample liquid, and the second calibration curve s ═ g (c) is used for representing the corresponding relation between the whole-process relative area and the concentration of the sample liquid.

In this embodiment, the first calibration curve r ═ f (c) and the second calibration curve s ═ g (c) are obtained by the test person performing specific protein analysis in the cuvette in advance through a standard sample with a known concentration, wherein the first calibration curve r ═ f (c) and the second calibration curve s ═ g (c) can be stored in a memory, and the control device reads from the memory.

Step 104, determining a first concentration C1 of the sample liquid corresponding to the full-range absolute area R based on the first calibration curve R ═ f (C); and determining a second concentration C2 of the sample liquid corresponding to the whole-course relative area S based on the second calibration curve S ═ g (C).

In the present embodiment, the first concentration C1 of the sample liquid corresponding to the full stroke absolute area R is obtained by substituting the full stroke absolute area R obtained in step S103 into the first calibration curve R ═ f (C). The second concentration C2 of the sample liquid corresponding to the full stroke relative area S can be obtained by substituting the full stroke relative area S obtained in step S103 into the second calibration curve S ═ g (C).

If the cuvette is in a normal working state (no protein attached), the first concentration C1 and the second concentration C2 should be equal, i.e. w-C2/C1 theoretically w-1. However, in practical situations, the second concentration C2 will be smaller and the first concentration C1 will be larger and larger, and thus w ═ C2/C1 will be smaller and smaller, for each specific protein analysis performed in the cuvette.

And 105, sending a reaction cup cleaning triggering instruction to the cleaning device according to the magnitude relation between the first concentration C1 and the second concentration C2, and cleaning the reaction cup.

In one embodiment, the step 105 of sending the cuvette washing trigger command to the washing device according to the magnitude relationship between the first concentration C1 and the second concentration C2 includes:

if the ratio w of the second concentration C2 to the first concentration C1 is C2/C1 and is less than or equal to a first preset threshold F1, sending a cup cleaning trigger command to the cleaning device, wherein the first preset threshold 0 is greater than F1 and is less than or equal to 1.

Thus, after the analysis of the sample to be tested is finished, if the ratio (w is C2/C1) of the second concentration C2 to the first concentration C1 is greater than the first preset threshold F1, the washing instruction is not triggered, that is, the reaction cup is not washed, the sample liquid in the reaction cup is directly drained and then the specific protein analysis of the next sample to be tested is performed, the reaction cup is not washed after each analysis is finished, the washing times are reduced, the analysis time is saved, and the reaction cup is washed only when the ratio (w is C2/C1) of the second concentration C2 to the first concentration C1 corresponding to a certain subsequent sample to be tested after the analysis is smaller than or equal to the first preset threshold F1.

In another embodiment, the step 105 of sending the cuvette washing trigger command to the washing device according to the magnitude relationship between the first concentration C1 and the second concentration C2 comprises:

and if the ratio w of the first concentration C1 to the second concentration C2 is equal to or greater than a second preset threshold value F2, sending a reaction cup cleaning triggering instruction to a cleaning device to clean the reaction cup.

Similarly, after the analysis of the sample to be tested is finished, if the ratio (w is C1/C2) of the first concentration C1 to the second concentration C2 is smaller than the first preset threshold F1, the washing instruction is not triggered, that is, the reaction cup is not washed, the sample liquid in the reaction cup is directly drained and then the specific protein of the next sample to be tested is analyzed, the reaction cup is not washed after each analysis is finished, the washing times are reduced, the analysis time is saved, and the reaction cup is washed only when the ratio (w is C1/C2) of the first concentration C1 to the second concentration C2 corresponding to a certain subsequent sample to be tested after the analysis is larger than or equal to the first preset threshold F1.

Referring to fig. 5, fig. 5 is a flowchart of an embodiment of a method for obtaining a first calibration curve and a second calibration curve, which may be completed before a blood cell analyzer leaves a factory, and the first calibration curve and the second calibration curve are stored in a memory, and may be directly retrieved from the memory when a subsequent user uses the blood cell analyzer, where the method includes steps 201 to 204, which are described in detail below.

Step 201, obtaining a plurality of standard samples with known concentrations, wherein the plurality of standard samples have different concentrations c₁,c₂,…,c_i,…,c_nWherein c is₁＜c₂＜…＜c_i＜…＜c_n。

Step 202, a plurality of standard samples are sequentially injected into a reaction cup for at least one specific protein analysis, and a plurality of standard reaction curves V ═ F are obtained_i(t)，i＝1,2,...,n。

In this embodiment, a plurality of standard samples are sequentially injected into the reaction cup for at least one specific protein analysis, including:

injecting a plurality of standard samples into the reaction cup for analyzing at least one specific protein in the following way:

injecting a standard sample into the reaction cup for at least one specific protein analysis; and after the analysis is finished, sending a reaction cup cleaning triggering instruction to the cleaning device to clean the reaction cup. In other words, after the specific protein analysis of each standard sample is finished, the reaction cup needs to be cleaned, so as to ensure that the accurate first calibration curve and the accurate second calibration curve are obtained.

Step (ii) of203, obtaining a plurality of standard reaction curves V ═ F_iAbsolute area of the whole course in (t)And relative area of the whole courseWhere T is the total length of time a standard sample is subjected to a particular protein assay. The concentration c can be obtained₁,c₂,…,c_i,…,c_nRespectively corresponding absolute area r of the whole course₁,r₂,…,r_i,…,r_nAnd a concentration c₁,c₂,…,c_i,…,c_nRespectively corresponding whole course relative area s₁,s₂,…,s_i,…,s_n。

204, according to the concentrations c of the plurality of standard samples₁,c₂,…,c_i,…,c_nAnd a plurality of global absolute areas r corresponding thereto₁,r₂,…,r_i,…,r_nFitting to obtain a first calibration curve; concentration c according to multiple standard samples₁,c₂,…,c_i,…,c_nAnd a plurality of corresponding global relative areas s₁,s₂,…,s_i,…,s_nAnd fitting to obtain a second calibration curve. The first calibration curve and the second calibration curve can be obtained by fitting by adopting the existing arbitrary curve fitting method.

Referring to fig. 6, fig. 6 is a schematic structural diagram of a specific protein analysis control device based on a blood cell analyzer according to an embodiment, wherein the control device 10 includes: a specific protein analysis unit 301, an area acquisition unit 302, a calibration curve acquisition unit 303, a concentration determination unit 304, and a wash trigger unit 305.

The specific protein analysis unit 301 is configured to inject a sample to be tested into the reaction cup to perform at least one specific protein analysis, and obtain a reaction curve of a sample solution in the reaction cup, where the reaction curve is used to represent a corresponding relationship between an electrical signal parameter corresponding to the sample solution and a specific protein analysis time in a specific protein analysis process.

The area obtaining unit 302 is configured to determine, according to the reaction curve, a whole-course absolute area and a whole-course relative area of the reaction curve, where the whole-course absolute area is used to represent an area of a region surrounded by the reaction curve, the electrical signal parameter axis, and the specific protein analysis time axis, and the whole-course relative area is used to represent a value range of the electrical signal parameter in the reaction curve, and an area of a region surrounded by the specific protein analysis time axis.

The calibration curve obtaining unit 303 is configured to obtain a first calibration curve and a second calibration curve, where the first calibration curve is used to represent a corresponding relationship between the absolute area of the whole process and the concentration of the sample liquid, and the second calibration curve is used to represent a corresponding relationship between the relative area of the whole process and the concentration of the sample liquid.

The concentration determination unit 304 is configured to determine a first concentration of the sample liquid corresponding to the whole absolute area based on the first calibration curve; and determining a second concentration of the sample liquid corresponding to the whole-course relative area based on the second calibration curve.

The cleaning triggering unit 305 is configured to send a cuvette cleaning triggering instruction to the cleaning device according to a magnitude relationship between the first concentration and the second concentration, so as to clean the cuvette.

Wherein, obtaining the first calibration curve and the second calibration curve comprises:

obtaining a plurality of standard samples with known concentrations, wherein the plurality of standard samples have different concentrations;

sequentially injecting a plurality of standard samples into the reaction cup for analyzing at least one specific protein to obtain a plurality of standard reaction curves;

acquiring whole-course absolute areas and whole-course relative areas in a plurality of standard reaction curves;

fitting according to the concentrations of the plurality of standard samples and the plurality of global absolute areas to obtain a first calibration curve; fitting according to the concentrations of the plurality of standard samples and the plurality of global relative areas to obtain a second calibration curve;

the step of sequentially injecting a plurality of standard samples into the reaction cup for at least one specific protein analysis comprises the following steps:

injecting a plurality of standard samples into the reaction cup for at least one specific protein analysis in the following way:

injecting a standard sample into the reaction cup for at least one specific protein analysis; and after the analysis is finished, sending a reaction cup cleaning triggering instruction to the cleaning device to clean the reaction cup.

According to the magnitude relation of the first concentration and the second concentration, sending a reaction cup cleaning triggering instruction to the cleaning device, wherein the method comprises the following steps:

if the ratio of the second concentration to the first concentration is smaller than or equal to a preset threshold value, sending a reaction cup cleaning trigger instruction to the cleaning device;

or if the ratio of the first concentration to the second concentration is greater than or equal to a preset threshold value, sending a reaction cup cleaning triggering instruction to the cleaning device.

The electrical signal parameter in this embodiment is a voltage value.

It should be noted that the specific protein analysis method provided in the foregoing embodiments is a method step of the specific protein analysis control apparatus of this embodiment, and specific embodiments thereof have been described in detail in the foregoing embodiments, and are not described herein again.

Those skilled in the art will appreciate that all or part of the functions of the various methods in the above embodiments may be implemented by hardware, or may be implemented by computer programs. When all or part of the functions of the above embodiments are implemented by a computer program, the program may be stored in a computer-readable storage medium, and the storage medium may include: a read only memory, a random access memory, a magnetic disk, an optical disk, a hard disk, etc., and the program is executed by a computer to realize the above functions. For example, the program may be stored in a memory of the device, and when the program in the memory is executed by the processor, all or part of the functions described above may be implemented. In addition, when all or part of the functions in the above embodiments are implemented by a computer program, the program may be stored in a storage medium such as a server, another computer, a magnetic disk, an optical disk, a flash disk, or a removable hard disk, and may be downloaded or copied to a memory of a local device, or may be version-updated in a system of the local device, and when the program in the memory is executed by a processor, all or part of the functions in the above embodiments may be implemented.

The present invention has been described in terms of specific examples, which are provided to aid understanding of the invention and are not intended to be limiting. For a person skilled in the art to which the invention pertains, several simple deductions, modifications or substitutions may be made according to the idea of the invention.