阅读说明：本技术 用于治疗组织坏死或改善心脏功能的药物 (Medicine for treating tissue necrosis or improving cardiac function ) 是由 钱进 孙宁 高翔 崔白苹 郑煜凡 于 2019-08-09 设计创作，主要内容包括：一种用于治疗缺血和缺氧引起的组织坏死或改善心脏功能的药物,该药物含有来自非人动物的羊水和/或其提取物,和任选的药学上可接受的载体,所述羊水来自于来自胚龄为5-12天的鸡蛋、发育时期相对应的其他禽类的蛋、胎龄为8-20天啮齿类动物的胚胎或发育时期相对应的其他非人哺乳动物的胚胎。能促进人脐静脉内皮细胞、AC16细胞和鸡胚胎成纤维细胞的生长,提高心肌梗塞后的心脏功能,促进心肌梗死后心脏细胞再生。(A medicament for treating tissue necrosis caused by ischemia and hypoxia or improving cardiac function, which comprises amniotic fluid derived from an egg with an embryonic age of 5-12 days, an egg of another bird corresponding to the development period, an embryo of a rodent with an embryonic age of 8-20 days or an embryo of another non-human mammal corresponding to the development period, and/or an extract thereof, and optionally a pharmaceutically acceptable carrier. Can promote the growth of human umbilical vein endothelial cells, AC16 cells and chicken embryo fibroblasts, improve the cardiac function after myocardial infarction and promote the regeneration of cardiac cells after myocardial infarction.)

A pharmaceutical composition, comprising:

amniotic fluid of non-human animals and/or extracts thereof, and

optionally a pharmaceutically acceptable carrier;

wherein the amniotic fluid is derived from an egg with an embryo age of 5-12 days, preferably an egg with an embryo age of 6-11 days, more preferably an egg with an embryo age of 7-9 days, more preferably an egg with an embryo age of 7-8 days, or an egg from a bird other than a chicken whose development period corresponds to the development period in which the embryo-aged egg is present; or an embryo from a rodent with a gestational age of 8-20 days, preferably 8-14 days, or an embryo from a non-human mammal other than a rodent whose developmental stage corresponds to that of a rodent with a gestational age of 8-20 days, preferably 8-14 days.

The pharmaceutical composition of claim 1, wherein the pharmaceutical composition is a cryopreserved amniotic fluid and/or an extract thereof, or is a lyophilized reagent of the amniotic fluid and/or the extract thereof.

The pharmaceutical composition of claim 1, wherein the pharmaceutical composition is an infusion solution.

The pharmaceutical composition of claim 3, wherein the pharmaceutical composition is free of or contains normal saline for injection, water for injection, or glucose injection solution.

The pharmaceutical composition according to any one of claims 1 to 4,

the amniotic fluid is from hen eggs, duck eggs, goose eggs or a combination thereof; or

The amniotic fluid is from a rodent that is pregnant for 10-14 days.

The pharmaceutical composition of any one of claims 1-5, wherein the extract is not bound to an ion exchange column at a pH of between 5.8 and 8.0 and comprises components having molecular weights in the range of 500 and 1200 daltons.

Application of amniotic fluid and/or its extract in preparing medicine for treating tissue necrosis caused by ischemia and anoxia, or in preparing medicine for improving cardiac function;

wherein the amniotic fluid is derived from an egg with an embryo age of 5-12 days, preferably an egg with an embryo age of 6-11 days, more preferably an egg with an embryo age of 7-9 days, more preferably an egg with an embryo age of 7-8 days, or an egg from a bird other than a chicken whose development period corresponds to the development period in which the embryo-aged egg is present; or an embryo from a rodent with a gestational age of 8-20 days, preferably 8-14 days, or an embryo from a non-human mammal other than a rodent whose developmental stage corresponds to that of a rodent with a gestational age of 8-20 days, preferably 8-14 days.

The use according to claim 7, wherein the medicament is for the treatment of myocardial ischemic necrosis, such as myocardial infarction; or the medicament is used for improving heart failure such as senile cardiac hypofunction or cardiac insufficiency.

The use according to any one of claims 7 to 8,

the amniotic fluid is from hen eggs, duck eggs, goose eggs or a combination thereof; or

The amniotic fluid is from a rodent that is pregnant for 10-14 days.

The use according to any one of claims 7 to 9, wherein the extract is not bound to an ion exchange column at a ph between 5.8 and 8.0 and comprises components having molecular weights in the range of 500 and 1200 daltons.