阅读说明：本技术 改善仔猪肠道健康的栗木单宁复合物及其制备方法和应用 (Chestnut tannin compound for improving intestinal health of piglets as well as preparation method and application thereof ) 是由 梁国安 于 2020-11-09 设计创作，主要内容包括：本发明公开了一种改善仔猪肠道健康的栗木单宁复合物,包括如下重量份的组分：栗木单宁150份-550份；酵母提取物50份-250份；有机酸或有机酸盐100份-250份；植物精油50份-150份；有机矿50份-150份。本发明能够提高仔猪的采食量并改善仔猪肠道健康,且其制备方法步骤简单,易生产,将其添加于猪饲料中可利于仔猪消化吸收,增强仔猪的免疫力,有效降低料重比和腹泻率,促进胃肠道的健康发育。本发明还提供该栗木单宁复合物的制备方法及其应用。(The invention discloses a chestnut tannin compound for improving intestinal health of piglets, which comprises the following components in parts by weight: 150-550 parts of chestnut tannin; 50-250 parts of yeast extract; 100-250 parts of organic acid or organic acid salt; 50-150 parts of plant essential oil; 50-150 parts of organic ore. The feed additive can improve the feed intake of piglets and improve the intestinal health of the piglets, has simple preparation method steps and easy production, can be easily digested and absorbed by the piglets when being added into the pig feed, enhances the immunity of the piglets, effectively reduces the feed-weight ratio and the diarrhea rate, and promotes the healthy development of the gastrointestinal tract. The invention also provides a preparation method and application of the chestnut tree tannin compound.)

1. A chestnut tannin compound for improving intestinal health of piglets is characterized by comprising the following components in parts by weight:

150-550 parts of chestnut tannin; 50-250 parts of yeast extract; 100-250 parts of organic acid or organic acid salt; 50-150 parts of plant essential oil; 50-150 parts of organic ore.

2. The chestnut tannin complex for improving intestinal health of piglets according to claim 1, which comprises the following components in parts by weight:

150-550 parts of chestnut tannin; 100-250 parts of yeast extract; 100-250 parts of organic acid or organic acid salt; 50-150 parts of plant essential oil; 80-120 parts of organic ore.

3. The chestnut tannin complex for improving intestinal health of piglets according to claim 1, wherein the chestnut tannin is extracted by using extraction technology, steam distillation, organic solvent extraction or supercritical CO₂The extraction technology is extracted from Castanea sativa L.

4. The chestnut tannin complex for improving intestinal health of piglets according to claim 3, wherein the chestnut tannin consists of chestnut lignin, sweet chestnut pigment, chestnut ellagic pigment and sweet chestnut ellagic pigment with the mass percentage of 3:8:25: 53.

5. Chestnut tannin complex according to claim 1, wherein the plant essential oil is one or more of cinnamaldehyde, eugenol, carvacrol, thymol.

6. The chestnut tannin complex for improving intestinal health of piglets according to claim 1, wherein the organic acid is one or a combination of formic acid, acetic acid, propionic acid, lactic acid, citric acid and fumaric acid; the organic acid salt is one or a combination of several of sodium formate, calcium formate, zinc formate, ammonium formate, iron formate, potassium formate and sodium acetate.

7. The chestnut tannin complex for improving intestinal health of piglets according to claim 1, wherein the organic minerals are one or more of ferrous lactate, zinc lactate, ferrous fumarate, copper glycinate, zinc methionine and selenium yeast.

8. A method for preparing chestnut tannin complexes as claimed in any one of claims 1 to 7, characterized in that it comprises the following preparation steps:

mixing yeast extract, organic acid or organic acid salt, plant essential oil, and organic ore, pulverizing, adding chestnut tannin, and stirring to obtain chestnut tannin complex.

9. Use of chestnut tannin complexes as claimed in any one of claims 1 to 7 in pig feed.

10. The use of chestnut tannin compound in pig feed according to claim 9, wherein the chestnut tannin compound is added to the pig feed in a weight percentage of 0.05% to 0.5%.

Technical Field

The invention relates to the technical field of feeds, in particular to a chestnut tannin compound for improving intestinal health of piglets and a preparation method and application thereof.

Background

With the increasingly deep public health breeding concept, the piglet breeding mode is gradually changed to a health and environment-friendly mode. However, due to the existence of intestinal diseases such as constipation and diarrhea of piglets, great economic loss is brought to the breeding production, and part of farmers even select antibiotics to treat the intestinal diseases of the piglets, so that serious harm is brought to human health.

Ellagitannins containing nonahydroxy-ditritoyl in chestnut tannin belong to the class of hydrolyzed tannins and contain glucosyl groups that are ring-opened and C-glycosidated. The study proves that the chestnut tannin has the effects of resisting bacteria, viruses and parasites. Because of its good disease prevention and growth promotion effects, chestnut tannin has been widely proposed as a first choice substitute for antibiotics. The chestnut tannin can improve the growth performance and the feed conversion rate of animals by increasing the secretion of endogenous digestive enzymes, maintaining the intestinal health and regulating the immunity, and in addition, the chestnut tannin also has the effect of oxidation resistance and can improve the quality of livestock and poultry products.

At present, chestnut tannin is mainly used for human medicine and health care, and is rarely used in animal (veterinary) medicines, and especially, chestnut tannin is used as a base, and a compound formed by the chestnut tannin and other materials is used as a product for improving the intestinal health of piglets, and is not disclosed and sold.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention provides the chestnut tannin compound for improving the intestinal health of the piglets, the chestnut tannin compound can realize high-value utilization of chestnut tannin, plant essential oil, organic acid or organic acid salt are matched in the formula, and after the materials are compounded, the chestnut tannin compound has better synergistic effect, can improve the feed intake of the piglets and improve the intestinal health of the piglets.

The invention also provides a preparation method of the chestnut tree tannin compound. The method has simple steps and is easy to produce.

The invention also provides an application of the chestnut tannin compound in pig feed. The feed additive is quantitatively fed to piglets, can enhance the immunity of the piglets, effectively reduce the feed-weight ratio and the diarrhea rate, and has a remarkable effect on improving the intestinal health of the piglets.

The invention is realized by adopting the following technical scheme:

a chestnut tannin compound for improving intestinal health of piglets comprises the following components in parts by weight:

150-550 parts of chestnut tannin; 50-250 parts of yeast extract; 100-250 parts of organic acid or organic acid salt; 50-150 parts of plant essential oil; 50-150 parts of organic ore.

Further, the chestnut tannin compound for improving intestinal health of piglets comprises the following components in parts by weight:

150-550 parts of chestnut tannin; 100-250 parts of yeast extract; 100-250 parts of organic acid or organic acid salt; 50-150 parts of plant essential oil; 80-120 parts of organic ore. Preferably, the mass ratio of the chestnut tannin to the yeast extract is 1-3: 1.

Under the optimal proportion, the feed added with the chestnut tannin compound has the best effect of solving intestinal tract diseases such as constipation and diarrhea of piglets and improving the immunity of the piglets.

Further, the chestnut tannin is prepared by adopting an extraction technology, a steam distillation method, an organic solvent extraction method or supercritical CO₂The extraction technology is extracted from Castanea sativa L.

Further, the chestnut tannin comprises chestnut lignin, sweet chestnut essence, chestnut ellagic essence and sweet chestnut ellagic essence with the mass percentage of 3:8:25: 53.

Further, the plant essential oil is one or a combination of more of cinnamaldehyde, eugenol, carvacrol and thymol.

The cinnamaldehyde has the effects of sterilization, disinfection and corrosion prevention, particularly has obvious curative effect on fungi, has an inhibiting effect on escherichia coli, bacillus subtilis, staphylococcus aureus, staphylococcus albus, shigella dysenteriae, typhoid and paratyphoid bacillus, pneumococcus, aerobacter, proteus, bacillus anthracis, salmonella enteritidis, vibrio cholerae and the like, has an obvious bactericidal effect on gram-positive bacteria, can be used for treating various diseases caused by bacterial infection, and has the Minimum Inhibitory Concentration (MIC) of 0.02-0.07 ul/ml and the MIC of 0.1-0.3 ul/ml for deep pathogenic fungi; japanese medical experts find that the cinnamaldehyde has obvious curative effect on fungi, and research on the cinnamaldehyde antifungal effect of 22 conditionally pathogenic fungi shows that: cinnamaldehyde is an antifungal active substance, and plays a role in sterilization mainly by destroying the cell wall of fungi, so that the medicament permeates into the fungi cell and destroys organelles. Therefore, the chestnut tannin complex can be used as one of formulas of chestnut tannin complexes in the embodiment, and can be used for treating diarrhea of livestock, particularly piglets, and improving intestinal health conditions of the piglets.

Further, the organic acid is one or a combination of several of formic acid, acetic acid, propionic acid, lactic acid, citric acid and fumaric acid; the organic acid salt is one or a combination of several of sodium formate, calcium formate, zinc formate, ammonium formate, iron formate, potassium formate and sodium acetate.

Further, the organic ore is one or a combination of more of ferrous lactate, zinc lactate, ferrous fumarate, copper glycinate, zinc methionine and selenium yeast.

Wherein the organic zinc such as zinc lactate can supplement zinc deficiency of young piglets, and enhance immunity and antifatigue ability of livestock.

The invention also provides a preparation method of the chestnut tannin compound, which comprises the following preparation steps:

mixing yeast extract, organic acid or organic acid salt, plant essential oil, and organic ore, pulverizing, adding chestnut tannin, and stirring to obtain chestnut tannin complex.

In the process of preparing the chestnut tannin compound, plant essential oil, organic acid and other substances are adsorbed and mixed, so that the mixing effect can be improved, meanwhile, the chestnut tannin compound in the feed is more beneficial to the feeding and intestinal absorption of piglets, the contact area of digestive enzymes in the piglets and the feed is also convenient to increase, and the absorption rate is improved. And then chestnut tannin is added into the crushed mixture for stirring, and because the chestnut tannin has good effects of preventing diseases and promoting growth and has the effects of resisting bacteria, viruses and coccidian, the chestnut tannin is attached to the outer surface of the mixture formed by the yeast extract, the organic acid or organic acid salt, the plant essential oil and the organic ore, the growth performance and the feed conversion rate of animals are improved by increasing the secretion of endogenous digestive enzyme, maintaining the health of intestinal tracts and regulating the immunity, and various harmful pathogenic bacteria causing diarrhea are inhibited and killed by improving the comprehensive immunity of livestock, so that the tension state of the intestines and the stomach is relieved, and the effects of diminishing inflammation and stopping diarrhea are finally achieved.

The invention also provides an application of the chestnut tannin compound in pig feed.

Further, the chestnut tannin compound is added into the pig feed according to the weight percentage of 0.05-0.5%. When in use, the feed additive can be dissolved in drinking water according to different growth stages of livestock, or can be directly added into feed to improve intestinal health of livestock, especially piglets. Preferably, the chestnut tannin compound is added into the pig feed according to the weight percentage of 0.5%. The feed containing the chestnut tannin compound in percentage by weight can save the use amount of plant essential oil and tannin and further save the cost on the basis of achieving the effect of improving the intestinal health of livestock.

Compared with the prior art, the invention has the beneficial effects that:

the feed additive can improve the feed intake of piglets and improve the intestinal health of the piglets, has simple preparation method steps and easy production, can be easily digested and absorbed by the piglets when being added into the pig feed, enhances the immunity of the piglets, effectively reduces the feed-weight ratio and the diarrhea rate, and promotes the healthy development of the gastrointestinal tract.

The chestnut tannin is added into daily ration, so that the growth performance and the welfare of animals can be improved. The main mechanism of chestnut tannin comprises: 1) exert a direct antibacterial effect (in particular inhibiting the growth of certain potential gram-negative pathogenic bacteria, such as escherichia coli 0157: h7 and staphylococcus aureus); 2) anticoccidial, antifungal or antioxidant properties; 3) an immune modulating function; 4) stimulating the secretion of digestive enzymes. In summary, chestnut tannin stimulates the production of peptides and digestive enzymes, such as lipases and amylases, which have a beneficial effect on the feed intake and nutrient availability of animals of different ages. In addition, they have antibacterial, anticoccidial, antifungal, antioxidant and immunoregulatory functions.

The plant essential oil contained in the chestnut tannin compound can stimulate salivary glands and gastric glands to secrete digestive juice, increase the appetite of livestock and promote digestion, has the functions of regulating intestines and contracting intestinal walls, and can promote the intestinal peristalsis of the livestock and eliminate inflammation. The yeast extract contains abundant nucleotide and yeast cell wall polysaccharide, and can stimulate appetite and improve immunity. Organic acids, whether single or mixed, have antimicrobial activity similar to antibiotics and can induce significant benefits to poultry, by inhibiting the proliferation of harmful bacteria such as escherichia coli, salmonella, campylobacter, etc., they can create an environment conducive to the proliferation of beneficial bacteria, improve the microflora of the gastrointestinal tract, and improve the growth performance of animals, primarily due to their broad spectrum of action, such as improved protein and energy availability (which may result from their reduced competition for host nutrient and endogenous nitrogen loss by the bacteria), reduced ammonia production and metabolism by other pathogenic bacteria that inhibit growth (to reduce the incidence of subclinical infections), and regulation and secretion of immune mediators. In addition, organic acids have several additional advantages over antibiotics, including the efficacy of lowering chyme pH, increasing pancreatic secretion, and improving nutrition to the gastrointestinal mucosa, improving gastrointestinal health. The organic mineral can supplement mineral element deficiency caused by inflammation or diarrhea. Furthermore, chestnut tannin is added into the formula, so that various harmful pathogenic bacteria causing diarrhea are inhibited and killed by improving the comprehensive immunity of livestock, the tension state of intestines and stomach is relieved, and finally the effects of diminishing inflammation and stopping diarrhea are achieved.

Detailed Description

The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments or technical features described below may form a new embodiment. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially. It should be noted that the substances contained in the chestnut tannin complex of the present invention are not limited to the above-mentioned substances, and that other substances may be added to the feed by the producer according to the different needs of the livestock, especially the piglets. The tannin extracts used in the following examples were extracted from castanea sativa by the extraction process described in "method for extracting vegetable tannins", western leather, volume 33, phase 20, month 10 2011. The tannin extract comprises castanein, castanein and castanein with the mass percentage of 3:8:25: 53.

The features and properties of the present invention are described in further detail below with reference to examples.

Example 1

Mixing 50 parts by weight of yeast extract, 250 parts by weight of citric acid, 50 parts by weight of cinnamaldehyde and 60 parts by weight of zinc lactate, crushing, adding 150 parts by weight of chestnut tannin, and uniformly stirring to obtain the chestnut tannin compound.

Example 2

Mixing 150 parts by weight of yeast extract, 250 parts by weight of citric acid, 100 parts by weight of cinnamaldehyde and 60 parts by weight of zinc lactate, crushing, adding 350 parts by weight of chestnut tannin, and uniformly stirring to obtain the chestnut tannin compound.

Example 3

Mixing 100 parts by weight of yeast extract, 150 parts by weight of citric acid, 80 parts by weight of cinnamaldehyde and 80 parts by weight of zinc lactate, crushing, adding 250 parts by weight of chestnut tannin, and uniformly stirring to obtain the chestnut tannin compound.

Example 4

Mixing 100 parts by weight of yeast extract, 150 parts by weight of citric acid, 60 parts by weight of cinnamaldehyde and 50 parts by weight of zinc lactate, crushing, adding 200 parts by weight of chestnut tannin, and uniformly stirring to obtain the chestnut tannin compound.

Example 5

Mixing 250 parts by weight of yeast extract, 250 parts by weight of citric acid, 100 parts by weight of eugenol and 80 parts by weight of zinc formate, crushing, adding 550 parts by weight of chestnut tannin, and uniformly stirring to obtain the chestnut tannin compound.

Comparative example 1

Mixing 250 parts by weight of citric acid, 100 parts by weight of eugenol and 80 parts by weight of zinc formate, crushing, adding 550 parts by weight of chestnut tannin, and uniformly stirring to obtain the additive.

Comparative example 2

250 parts by weight of yeast extract, 250 parts by weight of citric acid, 100 parts by weight of eugenol and 80 parts by weight of zinc formate are mixed, crushed and uniformly stirred to obtain the additive.

Comparative example 3

Mixing 250 parts by weight of yeast extract, 250 parts by weight of citric acid and 80 parts by weight of zinc formate, crushing, adding 550 parts by weight of chestnut tannin, and uniformly stirring to obtain the additive.

And (3) performance testing:

influence on growth performance of piglets

1. Test product

Treatments 1-5 and comparisons 1-3;

adding the product of the example 1 into feed according to the weight percentage of 0.2%, and mixing to obtain the feed of the treatment 1; adding the product of the embodiment 2 into feed according to the weight percentage of 0.1%, and mixing to obtain the feed of the treatment 2; adding the product of the embodiment 3 into feed according to the weight percentage of 0.15%, and mixing to obtain the feed of the treatment 3; adding the product of the embodiment 4 into feed according to the weight percentage of 0.3%, and mixing to obtain the feed of the treatment 4; adding the product of the example 5 into feed according to the weight percentage of 0.5%, and mixing to obtain the feed of the treatment 5; the products of comparative examples 1-3 were added to the feed at 0.5% by weight and mixed to obtain feeds of comparative examples 1, 2 and 3.

2. Experimental methods

900 healthy piglets with initial weight of 10 + -0.5 kg were randomly divided into 9 groups of 10 piglets each. Feeding piglets with the feed treated by 1-5 and the feed compared by 1-3, ensuring free feeding and drinking of the piglets, immunizing and expelling parasites according to a conventional mode, settling and weighing after 21 days, and carrying out statistical analysis on daily gain, daily feed intake, feed-weight ratio, intestinal inflammatory factor level and diarrhea rate; the blank control group is fed with feed without any feed additive, and the rest feeding conditions are the same as those of the treatment group.

3. Test results

The data of the test were calculated by using Microsoft Excel arrangement, and then analyzed for variance using SPSS20.0 software, and the results are shown in tables 1 to 4, respectively.

TABLE 1 Effect of feed on growth Performance of piglets

Name (R)	Daily average food intake (gram/head)	Daily gain (g/head)	Material to weight ratio	Diarrhea Rate (%)
					Control group	353±20.5	256±11.2	1.38±0.21	9.53
Comparative example 1	386±18.2	285±13.5	1.35±0.43	8.66
					Comparative example 2	369±24.3	266±10.6	1.37±0.17	7.32
Comparison 3	402±19.3	293±14.5	1.37±0.20	7.03
					Process 1	398±18.9	298±12.5	1.33±0.13	6.78
Treatment 2	401±22.2	328±13.7	1.22±0.23	5.87
					Treatment 3	421±16.6	339±21.2	1.24±0.15	6.12
Treatment 4	420±20.8	332±24.5	1.27±0.21	4.33
					Treatment 5	425±13.3	342±18.9	1.24±0.32	3.54

As can be seen from Table 1, compared with the blank control group and the comparisons 1-3, the addition of the chestnut tannin complex in the pig feed of the embodiment of the invention can significantly increase the average daily feed intake and average daily gain of piglets, and the feed-weight ratio and diarrhea rate are both significantly reduced.

TABLE 2 Effect of feed on the level of proinflammatory factor IL-1 beta (μ g/g protein) in the intestinal tract of piglets

Name (R)	Duodenum	Jejunum	Ileum
				Control group	11.23±0.45	2.88±0.33	3.01±0.11
Comparative example 1	10.86±0.55	2.71±0.24	2.91±0.43
				Comparative example 2	10.32±0.26	2.67±0.31	2.85±0.17
Comparison 3	9.91±0.48	2.61±0.22	2.80±0.20
				Process 1	9.23±0.41	2.55±0.21	2.78±0.31
Treatment 2	8.78±0.39	2.01±0.13	2.12±0.26
				Treatment 3	9.22±0.78	2.11±0.16	2.32±0.11
Treatment 4	9.54±0.21	1.98±0.24	2.54±0.45
				Treatment 5	8.94±0.43	2.31±0.32	2.09±0.17

As can be seen from Table 2, compared with the blank control group and the comparison groups 1-3, the addition of the chestnut tannin complex in the embodiment of the invention in the pig feed can significantly reduce the level of proinflammatory factor IL-1 beta in duodenum, jejunum and ileum of piglets.

TABLE 3 Effect of feed on the level of proinflammatory factor IL-2(μ g/g protein) in the intestinal tract of piglets

Name (R)	Duodenum	Jejunum	Ileum
				Control group	14.25±0.45	13.98±0.33	15.32±0.23
Comparative example 1	13.55±0.21	12.85±0.28	13.52±0.43
				Comparative example 2	12.96±0.30	12.53±0.25	13.21±0.24
Comparison 3	12.68±0.28	12.28±0.22	13.01±0.20
				Process 1	12.43±0.41	12.12±0.21	12.91±0.24
Treatment 2	9.86±0.22	12.34±0.23	10.24±0.31
				Treatment 3	9.02±0.54	11.51±0.27	11.74±0.65
Treatment 4	9.14±0.12	11.45±0.12	12.11±0.36
				Treatment 5	9.94±0.54	11.21±0.35	10.23±0.42

As can be seen from Table 3, compared with the blank control group and the comparison groups 1-3, the addition of the chestnut tannin complex in the embodiment of the invention in the pig feed can significantly reduce the level of proinflammatory factor IL-2 in duodenum, jejunum and ileum of piglets.

TABLE 4 influence of feed on the level of the intestinal anti-inflammatory factor IL-10(μ g/g protein) in piglets

Name (R)	Duodenum	Jejunum	Ileum
				Control group	234.13±1.23	107.25±0.43	195.36±0.25
Comparative example 1	241.22±1.28	115.68±0.28	198.52±1.43
				Comparative example 2	246.56±1.54	123.24±1.24	206.22±0.28
Comparison of3	251.12±1.28	141.02±1.45	208.11±1.23
				Process 1	289.52±0.76	212.17±0.21	242.95±10.24
Treatment 2	269.12±2.06	172.36±1.43	233.25±6.37
				Treatment 3	319.13±2.06	141.56±4.25	251.75±2.68
Treatment 4	319.13±6.92	161.76±3.22	229.10±2.37
				Treatment 5	259.49±2.45	151.78±4.25	210.53±5.47

As can be seen from Table 4, compared with the blank control group and the comparison groups 1-3, the addition of the chestnut tannin complex in the embodiment of the invention in the pig feed can significantly improve the level of the anti-inflammatory factor IL-10 in duodenum, jejunum and ileum of piglets.

The results in tables 1 to 4 show that the pig feed added with the chestnut tannin complex in the embodiment of the invention can significantly reduce the diarrhea rate and intestinal inflammation reaction of piglets compared with the pig feed without the chestnut tannin complex. Among treatments 1-5, treatment 5 had the best improvement in diarrhea and intestinal inflammatory response in piglets, because the feed contains chestnut tannin in the best proportion with yeast extract, organic acid or salt, plant essential oil and organic mineral, and the compatibility of the chestnut tannin with yeast extract, organic acid or salt, plant essential oil and organic mineral is correspondingly better.

In conclusion, the chestnut tannin compound provided by the embodiment of the invention can improve the feed intake of piglets and improve the intestinal health of the piglets, the preparation method is simple in steps and easy to produce, and the chestnut tannin compound can be added into pig feed to facilitate the digestion and absorption of the piglets, enhance the immunity of the piglets, effectively reduce the feed-weight ratio and the diarrhea rate, and promote the healthy development of the gastrointestinal tract.

The above embodiments are only a part of the preferred embodiments of the present invention, and the embodiments are described only for illustrating the principle of the present invention, and thus the scope of the present invention is not limited by the embodiments. As will be apparent to those skilled in the art, numerous changes, modifications and variations can be made in the present invention without departing from the spirit, principles and scope of the invention, the invention resides in the claims hereinafter appended, and the invention includes all such changes, modifications and variations.