阅读说明：本技术 一种利用南极磷虾活性钙制备的宠物营养膏及其制备方法 (Pet nutrition paste prepared from euphausia superba active calcium and preparation method thereof ) 是由 周庆新 刘婷婷 徐泽生 郭楚璇 王辉 尹利肖 陈芳甜 于 2020-12-24 设计创作，主要内容包括：本发明涉及宠物饲料技术领域,具体涉及一种利用南极磷虾活性钙制备的宠物营养膏及其制备方法,所述方法包括S1南极磷虾活性钙制备：以南极磷虾二级残渣作为原料,经过钙的释放、活性钙的转化和精制,得到南极磷虾活性钙溶液；S2营养膏赋型基质构建：所述营养膏赋型基质包括水系基料和油系物料,其中水系基料包括南极磷虾活性钙溶液；S3营养膏赋型基质混合：将水系基料和油系物料混合,形成水包油体系,得到赋型均匀的营养膏。本发明宠物营养膏以南极磷虾提取脂质和蛋白肽后剩余的南极磷虾二级残渣为原料,充分利用残渣中的钙和甲壳质资源,方法简单温和、绿色环保、耗能低,有利于产业化推广。(The invention relates to the technical field of pet feed, in particular to a pet nutrition paste prepared from euphausia superba active calcium and a preparation method thereof, wherein the method comprises the following steps of S1: taking the second-stage residue of the antarctic krill as a raw material, and obtaining an active calcium solution of the antarctic krill through the release of calcium, the conversion of active calcium and the refining; s2 construction of a forming matrix of the nutritional paste: the nutritional paste forming matrix comprises a water-based base material and an oil-based material, wherein the water-based base material comprises an antarctic krill active calcium solution; s3 mixing the nutritional paste forming matrix: mixing the water system base material and the oil system material to form an oil-in-water system, and obtaining the nutritional paste with uniform shape. The pet nutritional paste takes the secondary residue of the euphausia superba left after the lipid and the protein peptide are extracted from the euphausia superba as a raw material, fully utilizes calcium and chitin resources in the residue, is simple and mild, is green and environment-friendly, has low energy consumption, and is beneficial to industrial popularization.)

1. A method for preparing pet nutrition paste by using active calcium from Antarctic krill is characterized by comprising the following steps:

s1 preparation of activated calcium from Antarctic krill: taking the second-stage residue of the antarctic krill as a raw material, and obtaining an active calcium solution of the antarctic krill through the release of calcium, the conversion of active calcium and the refining;

s2 construction of a forming matrix of the nutritional paste: the nutritional paste forming matrix comprises a water-based base material and an oil-based material, wherein the water-based base material comprises an antarctic krill active calcium solution;

s3 mixing the nutritional paste forming matrix: mixing the water system base material and the oil system material to form an oil-in-water system, and obtaining the nutritional paste with uniform shape.

2. The method of claim 1, wherein the S1 Euphausia superba activated calcium preparation comprises:

(1) release of calcium: adding a citric acid aqueous solution into the secondary residue of the raw material euphausia superba, stirring and homogenizing, and performing ultrasonic auxiliary treatment to obtain a calcium release solution of the euphausia superba;

(2) conversion of activated calcium: adjusting the pH value of the antarctic krill calcium release solution to 7.0, adding the antarctic krill protein peptide solution, and carrying out ultrasonic treatment;

(3) refining: and (4) refining the solution after the ultrasonic treatment by using ultrafiltration, and collecting trapped fluid to obtain an active calcium solution of the euphausia superba.

3. The method of claim 2, wherein the S1 Antarctic krill activated calcium is prepared by:

(1) release of calcium: adding a citric acid aqueous solution into the raw material Antarctic krill secondary residue, wherein the mass ratio of the raw material to the citric acid aqueous solution is 1: 5-10, wherein the mass ratio of citric acid to water in the citric acid aqueous solution is 8-20: 100, stirring and homogenizing, and then performing ultrasonic treatment for 1-3 hours at the temperature of 30-50 ℃, wherein the ultrasonic treatment power is 160-240W, so as to obtain antarctic krill calcium release liquid;

(2) conversion of activated calcium: adding 1M sodium hydroxide solution into the antarctic krill calcium release solution, adjusting the pH to 7.0, and then adding the antarctic krill protein peptide solution, wherein the volume ratio of the antarctic krill calcium release solution to the antarctic krill protein peptide solution is 1: 0.2-0.5, and then performing ultrasonic treatment for 1-3 hours at the temperature of 50-70 ℃, wherein the ultrasonic treatment power is 160-240W;

(3) refining: and (3) refining the solution after the ultrasonic treatment by using ultrafiltration, wherein the ultrafiltration is carried out until the trapped fluid is 1/5-1/3 of the total volume of the original solution, the molecular interception weight of the ultrafiltration membrane is 200-300 Da, and the trapped fluid is collected to obtain the active calcium solution of the euphausia superba.

4. The method of claim 1, wherein the S2 nutritional paste forming matrix construction comprises:

(1) preparing a water system base material: mixing Euphausia superba active calcium solution, excipient, peptone, hydrolyzed animal protein, milk powder, glucose, maltose, white granulated sugar and vitamins, heating to dissolve after completely hydrating, and stirring uniformly to obtain water system base material;

(2) preparing oil materials: mixing animal fat, emulsifier, auxiliary emulsifier, and fat-soluble vitamins, stirring to disperse uniformly, and making into oil material.

5. The method of claim 4, wherein the S2 nutritional paste forming matrix is configured to:

(1) preparing a water system base material: weighing 100 parts of antarctic krill active calcium solution, 3-10 parts of excipient, 10-30 parts of peptone, 5-10 parts of hydrolyzed animal protein, 10-20 parts of milk powder, 10-20 parts of glucose, 5-10 parts of maltose, 10-20 parts of white granulated sugar and 0.5-2 parts of vitamin according to parts by weight, mixing, heating and dissolving at 40-90 ℃ after complete hydration, and uniformly stirring to prepare a water system base material;

the excipient comprises at least one of pectin, sodium alginate, gelatin, agar and xanthan gum;

(2) preparing oil materials: weighing 100 parts of animal fat, 1-10 parts of emulsifier, 0.1-5 parts of co-emulsifier and 0.1-2 parts of fat-soluble vitamin according to parts by weight, mixing, and stirring until the mixture is uniformly dispersed to prepare an oil material;

the animal oil comprises at least one of fish oil, shrimp oil, pork, chicken, beef tallow and mutton fat;

the emulsifier comprises at least one of sucrose ester, polyglycerol stearate, monoglyceride, diglycerol fatty acid ester, tween and lecithin.

6. The method according to claim 1, wherein the S3 nutritional paste forming base is mixed by:

respectively heating a water system base material and an oil system material to 70-100 ℃, and then slowly adding the oil system material into the water system base material, wherein the mass ratio of the water system base material to the oil system material is 1: 0.1-0.5, homogenizing and emulsifying while adding until the system is uniform, forming a stable oil-in-water system, and obtaining the nutritional paste with uniform shape without free oil materials.

7. The method according to claim 6, wherein the homogeneous emulsification is shear homogeneous emulsification, and the rotation speed of the shear homogeneous emulsification is 1000-30000 rpm for 5-30 min.

8. The method of claim 1, further comprising S4 sterilizing, packaging: and (3) preserving the heat of the nutrient paste at 65-70 ℃ for sterilization for 15min, and then subpackaging according to the production requirement.

9. A pet nutrition paste prepared by the method of claim 1.

10. The nutritional paste according to claim 9, wherein the mass fraction of active calcium from euphausia superba in the nutritional paste is not less than 500 mg/kg.

Technical Field

The invention relates to the technical field of pet feed, and particularly relates to a pet nutrition paste prepared from euphausia superba active calcium and a preparation method thereof.

Background

Pets, also known as "companion animals," play an increasingly important role in our lives as important human emotional setbacks, often viewed as one of the family members. Their nutrition and health have become a prominent challenge. Particularly, with the social progress, the concept of pet feeding is greatly changed, and the dietary requirements of people for pets are higher and higher. Therefore, the pet food industry is in need of transformation and upgrading, and particularly, the innovation of products is different according to the types and the requirements of pets, so that a more nutritional, healthier and more targeted pet food industry chain is developed. The pet nutrition paste is used as a quick-supplementing type nutriment which is easy to digest and absorb and is practical, is suitable for pets of all ages to eat, especially young and old pets, and has huge market demand prospect.

The biological reserves of the antarctic krill are huge, and the antarctic krill is an important strategic resource and belongs to one of three extremely important ocean resources to be developed. At present, Antarctic krill oil is most representative of a plurality of products developed by taking Antarctic krill as a raw material, however, Antarctic krill powder, which is a residue left after the Antarctic krill oil is extracted, is mostly used as a feed raw material, and the added value of the product is low. Therefore, research on preparing a pet nutrition paste rich in antarctic krill lipid and a pet nutrition paste rich in antarctic krill protein peptide by using antarctic krill meal as a raw material has been developed, so that lipid and protein resources in residues are utilized, but the residual residues are not effectively developed.

Therefore, the development of the pet nutritional paste rich in active calcium and prepared by utilizing the residues generated in the processing process of the euphausia superba powder and the preparation method thereof are very important for further improving the comprehensive utilization rate of euphausia superba resources, realizing the comprehensive utilization of the resources and improving the added value of products.

Disclosure of Invention

The pet nutrition paste takes the second-stage residue of the Antarctic krill left after the extraction of lipid and protein peptide from the Antarctic krill as a raw material, and fully utilizes calcium and chitin resources in the residue.

In a first aspect, the present invention provides a method for preparing a pet nutritional paste from activated calcium from euphausia superba, the method comprising:

s1 preparation of activated calcium from Antarctic krill: taking the second-stage residue of the antarctic krill as a raw material, and obtaining an active calcium solution of the antarctic krill through the release of calcium, the conversion of active calcium and the refining;

s2 construction of a forming matrix of the nutritional paste: the nutritional paste forming matrix comprises a water-based base material and an oil-based material, wherein the water-based base material comprises an antarctic krill active calcium solution;

s3 mixing the nutritional paste forming matrix: mixing the water system base material and the oil system material to form an oil-in-water system, and obtaining the nutritional paste with uniform shape.

Further, the second-stage residue of the antarctic krill refers to residue left after preparing the pet nutrition paste rich in the antarctic krill lipid and the pet nutrition paste rich in the antarctic krill protein peptide, and specifically is solid residue left after extracting the antarctic krill powder with ethanol and performing enzymolysis with acid protease.

Further, the preparation of the S1 antarctic krill activated calcium comprises:

(1) release of calcium: adding a citric acid aqueous solution into the secondary residue of the raw material euphausia superba, stirring and homogenizing, and performing ultrasonic auxiliary treatment to obtain a calcium release solution of the euphausia superba;

(2) conversion of activated calcium: adjusting the pH value of the antarctic krill calcium release solution to 7.0, adding the antarctic krill protein peptide solution, and carrying out ultrasonic treatment;

(3) refining: and (4) refining the solution after the ultrasonic treatment by using ultrafiltration, and collecting trapped fluid to obtain an active calcium solution of the euphausia superba.

Further, the preparation of the S1 antarctic krill activated calcium specifically comprises the following steps:

(1) release of calcium: adding a citric acid aqueous solution into the raw material Antarctic krill secondary residue, wherein the mass ratio of the raw material to the citric acid aqueous solution is 1: 5-10, wherein the mass ratio of citric acid to water in the citric acid aqueous solution is 8-20: 100, stirring and homogenizing, and then performing ultrasonic treatment for 1-3 hours at the temperature of 30-50 ℃, wherein the ultrasonic treatment power is 160-240W, so as to obtain antarctic krill calcium release liquid;

(2) conversion of activated calcium: adding 1M sodium hydroxide solution into the antarctic krill calcium release solution, adjusting the pH to 7.0, and then adding the antarctic krill protein peptide solution, wherein the volume ratio of the antarctic krill calcium release solution to the antarctic krill protein peptide solution is 1: 0.2-0.5, and then performing ultrasonic treatment for 1-3 hours at the temperature of 50-70 ℃, wherein the ultrasonic treatment power is 160-240W;

(3) refining: and (3) refining the solution after the ultrasonic treatment by using ultrafiltration, wherein the ultrafiltration is carried out until the trapped fluid is 1/5-1/3 of the total volume of the original solution, the molecular interception weight of the ultrafiltration membrane is 200-300 Da, and the trapped fluid is collected to obtain the active calcium solution of the euphausia superba.

Further, the antarctic krill protein peptide solution is a protein peptide solution obtained by pretreating, carrying out enzymolysis combined debittering, refining and concentrating first-stage residues of the antarctic krill remaining after the preparation of the pet nutrition paste rich in the antarctic krill lipids, and the specific preparation method comprises the following steps:

(a) pretreatment of raw materials: adding a citric acid aqueous solution into the first-stage residue of the raw material euphausia superba, uniformly stirring, and then adding acid protease for enzymolysis to obtain a treatment solution A;

(b) enzymolysis and combined debitterizing: carrying out enzymolysis on the treatment solution A by using flavourzyme and trypsin, adding sugar into the system after the enzymolysis is finished, stirring, dissolving and preserving heat to obtain a treatment solution B;

(c) refining: refining the treated liquid B by ultrafiltration, and collecting filtrate to obtain treated liquid C;

(d) concentration: and (4) carrying out reduced pressure concentration treatment on the treated liquid C to obtain the euphausia superba protein peptide solution.

Further, the first-stage residue of the antarctic krill refers to residue left after the preparation of the pet nutrition paste rich in the antarctic krill lipid, and the specific preparation method comprises the following steps: and adding an extraction solvent into the Antarctic krill powder, wherein the solid residue after extraction is the first-grade residue of the Antarctic krill.

Further, the S2 nutritional paste forming matrix construction comprises:

(1) preparing a water system base material: mixing Euphausia superba active calcium solution, excipient, peptone, hydrolyzed animal protein, milk powder, glucose, maltose, white granulated sugar and vitamins, heating to dissolve after completely hydrating, and stirring uniformly to obtain water system base material;

(2) preparing oil materials: mixing animal fat, emulsifier, auxiliary emulsifier, and fat-soluble vitamins, stirring to disperse uniformly, and making into oil material.

Further, the S2 nutritional paste forming matrix is specifically constructed by the following steps:

(1) preparing a water system base material: weighing 100 parts of antarctic krill active calcium solution, 3-10 parts of excipient, 10-30 parts of peptone, 5-10 parts of hydrolyzed animal protein, 10-20 parts of milk powder, 10-20 parts of glucose, 5-10 parts of maltose, 10-20 parts of white granulated sugar and 0.5-2 parts of vitamin according to parts by weight, mixing, heating and dissolving at 40-90 ℃ after complete hydration, and uniformly stirring to prepare a water system base material;

the excipient comprises at least one of pectin, sodium alginate, gelatin, agar and xanthan gum;

(2) preparing oil materials: weighing 100 parts of animal fat, 1-10 parts of emulsifier, 0.1-5 parts of co-emulsifier and 0.1-2 parts of fat-soluble vitamin according to parts by weight, mixing, and stirring until the mixture is uniformly dispersed to prepare an oil material;

the animal oil comprises at least one of fish oil, shrimp oil, pork, chicken, beef tallow and mutton fat;

the emulsifier comprises at least one of sucrose ester, polyglycerol stearate, monoglyceride, diglycerol fatty acid ester, tween and lecithin.

Further, the S3 nutritional paste forming matrix is specifically mixed as follows:

respectively heating a water system base material and an oil system material to 70-100 ℃, and then slowly adding the oil system material into the water system base material, wherein the mass ratio of the water system base material to the oil system material is 1: 0.1-0.5, homogenizing and emulsifying while adding until the system is uniform, forming a stable oil-in-water system, and obtaining the nutritional paste with uniform shape without free oil materials.

Further, the homogeneous emulsification is shearing homogeneous emulsification, the rotating speed of the shearing homogeneous emulsification is 1000-30000 rpm, and the time is 5-30 min.

Further, the method also comprises the steps of S4 sterilization and packaging: and (3) preserving the heat of the nutrient paste at 65-70 ℃ for sterilization for 15min, and then subpackaging according to the production requirement.

In a second aspect, the invention provides a pet nutrition paste prepared by the above method.

Furthermore, the mass fraction of the active calcium of the euphausia superba in the nutritional paste is not less than 500 mg/kg.

The nutritional paste product rich in the active calcium of the euphausia superba, which is good in nutrition, efficacy and palatability, is prepared by taking the comprehensive utilization of the euphausia superba resources as a starting point, utilizing the residues generated in the processing process, preparing the active calcium of the euphausia superba through the processes of calcium release, calcium conversion and the like, and then through the formula matrix effect and the shaping design, so that the development of high-end pet nutrition products in China is promoted, the comprehensive utilization rate of the euphausia superba is improved, and the added value of the product is improved.

The pet nutrition paste prepared from the active calcium of the Antarctic krill has the characteristics of natural raw materials, comprehensive nutrition function and rich flavor. The product of the invention is rich in active calcium, has high absorption and utilization rate of calcium, and can effectively prevent the occurrence of bone diseases such as osteomalacia, rickets, osteoporosis, postpartum paralysis and the like of pets.

The preparation process is easy to operate, and the final product has good stability, high calcium absorption and utilization rate and no oil-water separation phenomenon after long-time storage by designing a formula and optimizing a process.

Drawings

In order to more clearly illustrate the embodiments or technical solutions in the prior art of the present invention, the drawings used in the description of the embodiments or prior art will be briefly described below, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.

Fig. 1 is a flow chart of a method for preparing a pet nutrition paste by using antarctic krill activated calcium in the embodiment of the invention.

Detailed Description

In order to make those skilled in the art better understand the technical solution of the present invention, the technical solution in the embodiment of the present invention will be clearly and completely described below with reference to the drawings in the embodiment of the present invention, and it is obvious that the described embodiment is only a part of the embodiment of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

A pet nutrition paste prepared from euphausia superba active calcium is prepared by the following steps:

s1 preparation of activated calcium from Antarctic krill:

(1) release of calcium: adding a citric acid aqueous solution into the second-stage residue of the raw material Antarctic krill, wherein the mass ratio of the raw material to the citric acid aqueous solution is 1: 5, the mass ratio of citric acid to water in the citric acid aqueous solution is 8: 100, stirring and homogenizing, and then performing ultrasonic treatment for 1h at the temperature of 30 ℃, wherein the ultrasonic treatment power is 160W, so as to obtain antarctic krill calcium release liquid;

the second-stage residue of the antarctic krill is residue left after preparing the pet nutrition paste rich in the antarctic krill lipid and the pet nutrition paste rich in the antarctic krill protein peptide, and specifically is solid residue left after extracting the antarctic krill powder with ethanol and performing enzymolysis with acid protease;

the specific preparation method of the first-stage residue of the Antarctic krill comprises the following steps:

adding an ethanol water solution with the concentration of 0.85g/L into the Antarctic krill powder, wherein the volume ratio of the ethanol water solution to the Antarctic krill powder is 2: 1, extracting for 1 time for 0.5h, and collecting the extracted solid residue, namely the first-stage residue of the euphausia superba;

the specific preparation method of the second-stage residue of the Antarctic krill comprises the following steps:

adding a citric acid aqueous solution into the first-stage residue of the euphausia superba, wherein the mass ratio of the first-stage residue of the euphausia superba to the citric acid aqueous solution is 1: 3, the mass ratio of citric acid to water in the citric acid aqueous solution is 0.015: 100, adding acid protease for enzymolysis after uniformly stirring, wherein the concentration of the acid protease is 500u/g, stirring for 2 hours at 35 ℃, and collecting solid residues after enzymolysis, namely second-stage residues of antarctic krill;

(2) conversion of activated calcium: adding 1M sodium hydroxide solution into the antarctic krill calcium release solution, adjusting the pH to 7.0, and then adding the antarctic krill protein peptide solution, wherein the volume ratio of the antarctic krill calcium release solution to the antarctic krill protein peptide solution is 1: 0.2, then carrying out ultrasonic treatment for 1h at the temperature of 50 ℃, wherein the ultrasonic treatment power is 160W;

the euphausia superba protein peptide solution is obtained by pretreating, carrying out enzymolysis combined debittering, refining and concentrating first-stage residue of euphausia superba left after preparing the pet nutrition paste rich in euphausia superba lipid, and the specific preparation method comprises the following steps:

(a) pretreatment of raw materials: adding a citric acid aqueous solution into the first-stage residue of the euphausia superba, wherein the mass ratio of the first-stage residue of the euphausia superba to the citric acid aqueous solution is 1: 3, the mass ratio of citric acid to water in the citric acid aqueous solution is 0.015: 100, adding acid protease for enzymolysis after uniformly stirring, wherein the concentration of the acid protease is 500u/g, the enzymolysis condition is stirring for 2 hours at 35 ℃, and obtaining a treatment solution A after the enzymolysis is finished;

(b) enzymolysis and combined debitterizing: adding 1M sodium hydroxide solution into the treatment liquid A to adjust the pH value to 7.0, then adding flavourzyme and trypsin to carry out enzymolysis on the treatment liquid A, wherein the concentration of the flavourzyme is 100u/g, the concentration of the trypsin is 50u/g, the enzymolysis condition is stirring for 2 hours at 35 ℃, adding sugar into a system after the enzymolysis is finished, and the mass ratio of the sugar to the system after the enzymolysis is 1: 100, stirring and dissolving sugar including glucose, heating the system to 90 ℃, preserving heat for 1h, and cooling to room temperature after heat preservation to obtain a treatment solution B;

(c) refining: refining the treatment solution B by ultrafiltration, wherein the molecular interception of the ultrafiltration membrane is 2000-3000 Da, and collecting the filtrate after the ultrafiltration is finished to obtain a treatment solution C;

(d) concentration: carrying out reduced pressure concentration treatment on the treatment liquid C under the conditions of vacuum degree of-0.06 MPa and 60 ℃ to ensure that the content of solid matters in a liquid system is not lower than 20 percent, thus obtaining a protein peptide solution of the antarctic krill;

(3) refining: refining the solution after ultrasonic treatment by using ultrafiltration until the trapped fluid is 1/5 of the total volume of the original solution, wherein the molecular interception of the ultrafiltration membrane is 200-300 Da, and collecting the trapped fluid to obtain an active calcium solution of antarctic krill;

s2 construction of a forming matrix of the nutritional paste:

(1) preparing a water system base material: weighing 100 parts of antarctic krill active calcium solution, 3 parts of excipient, 10 parts of peptone, 5 parts of hydrolyzed animal protein, 10 parts of milk powder, 10 parts of glucose, 5 parts of maltose, 10 parts of white granulated sugar and 0.5 part of vitamin according to parts by weight, mixing, heating and dissolving at 40 ℃ after complete hydration, and uniformly stirring to prepare a water-system base material, wherein the excipient comprises pectin;

(2) preparing oil materials: weighing 100 parts of animal fat, 1 part of emulsifier, 0.1 part of co-emulsifier and 0.1 part of fat-soluble vitamin according to the parts by weight, mixing, stirring until the mixture is uniformly dispersed, and preparing an oil material, wherein the animal fat comprises beef tallow, and the emulsifier comprises sucrose ester;

s3 mixing the nutritional paste forming matrix: respectively heating the water system base material and the oil system material to 70 ℃, and then slowly adding the oil system material into the water system base material, wherein the mass ratio of the water system base material to the oil system material is 1: 0.1, adding while homogenizing and emulsifying, wherein the homogenizing and emulsifying is shearing homogenizing and emulsifying, the rotating speed of the shearing homogenizing and emulsifying is 1000rpm, and the time is 5min, until the system is uniform, a stable oil-in-water system is formed, free oil materials are not generated, and the uniformly-shaped nutritional paste is obtained;

s4, sterilization and packaging: sterilizing the nutritional paste at 65 deg.C for 15min, and packaging according to production requirement.

The edible method of the nutritional paste in the embodiment 1 is to eat the nutritional paste instantly after uncovering, and the nutritional paste is about 1-5 g per day according to nutritional requirements.

Example 1 sensory indices of the nutritional cream are as follows:

color: the color is yellow brown or dark brown, and is uniform and consistent;

taste and smell: the flavor is strong, and the product can be melted in the mouth;

the form is as follows: the paste is semisolid, and the phenomenon of phase separation does not occur after the paste is placed for a long time;

impurities: no visible impurities.

Example 2

A pet nutrition paste prepared from euphausia superba active calcium is prepared by the following steps:

s1 preparation of activated calcium from Antarctic krill:

(1) release of calcium: adding a citric acid aqueous solution into the second-stage residue of the raw material Antarctic krill, wherein the mass ratio of the raw material to the citric acid aqueous solution is 1: 10, the mass ratio of citric acid to water in the citric acid aqueous solution is 20: 100, stirring and homogenizing, and then performing ultrasonic treatment for 3 hours at 50 ℃, wherein the ultrasonic treatment power is 240W, so as to obtain antarctic krill calcium release liquid;

the second-stage residue of the antarctic krill is residue left after preparing the pet nutrition paste rich in the antarctic krill lipid and the pet nutrition paste rich in the antarctic krill protein peptide, and specifically is solid residue left after extracting the antarctic krill powder with ethanol and performing enzymolysis with acid protease;

the specific preparation method of the first-stage residue of the Antarctic krill comprises the following steps:

adding 1g/L ethanol water solution into the Antarctic krill powder, wherein the volume ratio of the ethanol water solution to the Antarctic krill powder is 10: 1, extracting for 3 times for 4 hours, and collecting the extracted solid residue to obtain first-stage residue of the euphausia superba;

the specific preparation method of the second-stage residue of the Antarctic krill comprises the following steps:

adding a citric acid aqueous solution into the first-stage residue of the euphausia superba, wherein the mass ratio of the first-stage residue of the euphausia superba to the citric acid aqueous solution is 1: 5, the mass ratio of citric acid to water in the citric acid aqueous solution is 0.1: 100, adding acid protease for enzymolysis after uniformly stirring, wherein the concentration of the acid protease is 1000u/g, the enzymolysis condition is stirring for 4 hours at 45 ℃, and collecting solid residue after enzymolysis, namely second-stage residue of antarctic krill;

(2) conversion of activated calcium: adding 1M sodium hydroxide solution into the antarctic krill calcium release solution, adjusting the pH to 7.0, and then adding the antarctic krill protein peptide solution, wherein the volume ratio of the antarctic krill calcium release solution to the antarctic krill protein peptide solution is 1: 0.5, then carrying out ultrasonic treatment for 3 hours at the temperature of 70 ℃, wherein the ultrasonic treatment power is 240W;

the euphausia superba protein peptide solution is obtained by pretreating, carrying out enzymolysis combined debittering, refining and concentrating first-stage residue of euphausia superba left after preparing the pet nutrition paste rich in euphausia superba lipid, and the specific preparation method comprises the following steps:

(a) pretreatment of raw materials: adding a citric acid aqueous solution into the first-stage residue of the euphausia superba, wherein the mass ratio of the first-stage residue of the euphausia superba to the citric acid aqueous solution is 1: 5, the mass ratio of citric acid to water in the citric acid aqueous solution is 0.1: 100, adding acid protease for enzymolysis after uniformly stirring, wherein the concentration of the acid protease is 1000u/g, the enzymolysis condition is stirring for 4 hours at 45 ℃, and obtaining a treatment solution A after the enzymolysis is finished;

(b) enzymolysis and combined debitterizing: adding 1M sodium hydroxide solution into the treatment liquid A to adjust the pH value to 8.0, then adding flavourzyme and trypsin to carry out enzymolysis on the treatment liquid A, wherein the concentration of the flavourzyme is 200u/g, the concentration of the trypsin is 100u/g, the enzymolysis condition is stirring for 4 hours at 45 ℃, adding sugar into a system after the enzymolysis is finished, and the mass ratio of the sugar to the system after the enzymolysis is 10: 100, stirring and dissolving sugar including xylose, heating the system to 100 ℃, preserving heat for 4 hours, and cooling to room temperature after heat preservation to obtain a treatment solution B;

(c) refining: refining the treatment solution B by ultrafiltration, wherein the molecular interception of the ultrafiltration membrane is 2000-3000 Da, and collecting the filtrate after the ultrafiltration is finished to obtain a treatment solution C;

(d) concentration: carrying out reduced pressure concentration treatment on the treatment liquid C under the conditions of vacuum degree of 0.09MPa and temperature of 90 ℃ to ensure that the content of solid matters in a liquid system is not lower than 20 percent, thus obtaining a protein peptide solution of the antarctic krill;

(3) refining: refining the solution after ultrasonic treatment by using ultrafiltration until the trapped fluid is 1/3 of the total volume of the original solution, wherein the molecular interception of the ultrafiltration membrane is 200-300 Da, and collecting the trapped fluid to obtain an active calcium solution of antarctic krill;

s2 construction of a forming matrix of the nutritional paste:

(1) preparing a water system base material: weighing 100 parts of antarctic krill active calcium solution, 10 parts of excipient, 30 parts of peptone, 10 parts of hydrolyzed animal protein, 20 parts of milk powder, 20 parts of glucose, 10 parts of maltose, 20 parts of white granulated sugar and 2 parts of vitamin according to parts by weight, mixing, heating and dissolving at 90 ℃ after complete hydration, and uniformly stirring to prepare a water-system base material, wherein the excipient comprises gelatin;

(2) preparing oil materials: weighing 100 parts of animal fat, 10 parts of emulsifier, 5 parts of co-emulsifier and 2 parts of fat-soluble vitamin according to the parts by weight, mixing, stirring until the mixture is uniformly dispersed, and preparing an oil material, wherein the animal fat comprises mutton fat, and the emulsifier comprises tween;

s3 mixing the nutritional paste forming matrix: respectively heating the water system base material and the oil system material to 100 ℃, and then slowly adding the oil system material into the water system base material, wherein the mass ratio of the water system base material to the oil system material is 1: 0.5, adding while homogenizing and emulsifying, wherein the homogenizing and emulsifying is shearing homogenizing and emulsifying, the rotating speed of the shearing homogenizing and emulsifying is 30000rpm, the time is 30min, until the system is uniform, a stable oil-in-water system is formed, free oil materials are not generated, and the uniformly-shaped nutritional paste is obtained;

s4, sterilization and packaging: sterilizing the nutritional paste at 70 deg.C for 15min, and packaging according to production requirement.

The edible method of the nutritional paste in the embodiment 2 is to eat the nutritional paste instantly after uncovering, and the nutritional paste is about 1-5 g per day according to nutritional requirements.

Example 2 sensory indices of the nutritional cream are as follows:

color: the color is yellow brown or dark brown, and is uniform and consistent;

taste and smell: the flavor is strong, and the product can be melted in the mouth;

the form is as follows: the paste is semisolid, and the phenomenon of phase separation does not occur after the paste is placed for a long time;

impurities: no visible impurities.

Example 3

A pet nutrition paste prepared from euphausia superba active calcium is prepared by the following steps:

s1 preparation of activated calcium from Antarctic krill:

(1) release of calcium: adding a citric acid aqueous solution into the second-stage residue of the raw material Antarctic krill, wherein the mass ratio of the raw material to the citric acid aqueous solution is 1: 7.5, the mass ratio of citric acid to water in the citric acid aqueous solution is 15: 100, stirring and homogenizing, and then performing ultrasonic treatment for 2 hours at the temperature of 30-50 ℃, wherein the ultrasonic treatment power is 200W, so as to obtain antarctic krill calcium release liquid;

the second-stage residue of the antarctic krill is residue left after preparing the pet nutrition paste rich in the antarctic krill lipid and the pet nutrition paste rich in the antarctic krill protein peptide, and specifically is solid residue left after extracting the antarctic krill powder with ethanol and performing enzymolysis with acid protease;

the specific preparation method of the first-stage residue of the Antarctic krill comprises the following steps:

adding 1g/L ethanol water solution into the Antarctic krill powder, wherein the volume ratio of the ethanol water solution to the Antarctic krill powder is 6: 1, extracting for 2 times for 2 hours, and collecting the extracted solid residue to obtain first-stage residue of the euphausia superba;

the specific preparation method of the second-stage residue of the Antarctic krill comprises the following steps:

adding a citric acid aqueous solution into the first-stage residue of the euphausia superba, wherein the mass ratio of the first-stage residue of the euphausia superba to the citric acid aqueous solution is 1: 4, the mass ratio of citric acid to water in the citric acid aqueous solution is 0.05: 100, adding acid protease for enzymolysis after uniformly stirring, wherein the concentration of the acid protease is 800u/g, the enzymolysis condition is stirring for 3 hours at 40 ℃, and collecting solid residue after enzymolysis, namely second-stage residue of antarctic krill;

(2) conversion of activated calcium: adding 1M sodium hydroxide solution into the antarctic krill calcium release solution, adjusting the pH to 7.0, and then adding the antarctic krill protein peptide solution, wherein the volume ratio of the antarctic krill calcium release solution to the antarctic krill protein peptide solution is 1: 0.3, then carrying out ultrasonic treatment for 2h at the temperature of 60 ℃, wherein the ultrasonic treatment power is 200W;

the euphausia superba protein peptide solution is obtained by pretreating, carrying out enzymolysis combined debittering, refining and concentrating first-stage residue of euphausia superba left after preparing the pet nutrition paste rich in euphausia superba lipid, and the specific preparation method comprises the following steps:

(a) pretreatment of raw materials: adding a citric acid aqueous solution into the first-stage residue of the euphausia superba, wherein the mass ratio of the first-stage residue of the euphausia superba to the citric acid aqueous solution is 1: 4, the mass ratio of citric acid to water in the citric acid aqueous solution is 0.05: 100, adding acid protease for enzymolysis after uniformly stirring, wherein the concentration of the acid protease is 800u/g, the enzymolysis condition is stirring for 3 hours at 40 ℃, and obtaining a treatment solution A after the enzymolysis is finished;

(b) enzymolysis and combined debitterizing: adding 1M sodium hydroxide solution into the treatment liquid A to adjust the pH value to 7.5, then adding flavourzyme and trypsin to carry out enzymolysis on the treatment liquid A, wherein the concentration of the flavourzyme is 150u/g, the concentration of the trypsin is 75u/g, the enzymolysis condition is stirring for 3 hours at 40 ℃, adding sugar into a system after the enzymolysis is finished, and the mass ratio of the sugar to the system after the enzymolysis is 6: 100, stirring and dissolving sugar comprising fructose, heating the system to 100 ℃, preserving heat for 3 hours, and cooling to room temperature after heat preservation to obtain a treatment solution B;

(c) refining: refining the treatment solution B by ultrafiltration, wherein the molecular interception of the ultrafiltration membrane is 2000-3000 Da, and collecting the filtrate after the ultrafiltration is finished to obtain a treatment solution C;

(d) concentration: carrying out reduced pressure concentration treatment on the treatment liquid C under the conditions of vacuum degree of-0.03 MPa and 80 ℃ to ensure that the content of solid matters in a liquid system is not lower than 20 percent, thus obtaining a protein peptide solution of the antarctic krill;

(3) refining: refining the solution after ultrasonic treatment by using ultrafiltration until the trapped fluid is 1/4 of the total volume of the original solution, wherein the molecular interception of the ultrafiltration membrane is 200-300 Da, and collecting the trapped fluid to obtain an active calcium solution of antarctic krill;

s2 construction of a forming matrix of the nutritional paste:

(1) preparing a water system base material: weighing 100 parts of antarctic krill active calcium solution, 6 parts of excipient, 20 parts of peptone, 8 parts of hydrolyzed animal protein, 15 parts of milk powder, 15 parts of glucose, 7 parts of maltose, 15 parts of white granulated sugar and 1 part of vitamin according to parts by weight, mixing, heating and dissolving at 60 ℃ after complete hydration, and uniformly stirring to prepare a water-system base material, wherein the excipient comprises the following components in parts by weight: 1 sodium alginate and agar;

(2) preparing oil materials: weighing 100 parts of animal fat, 5 parts of emulsifier, 2.5 parts of co-emulsifier and 1 part of fat-soluble vitamin according to the parts by weight, mixing, stirring until the mixture is uniformly dispersed, and preparing the oil material, wherein the animal fat comprises the components in a volume ratio of 1: 3, the emulsifier comprises the following components in a mass ratio of 1: 1 monoglyceride and diglycerol fatty acid esters;

s3 mixing the nutritional paste forming matrix: respectively heating the water system base material and the oil system material to 80 ℃, and then slowly adding the oil system material into the water system base material, wherein the mass ratio of the water system base material to the oil system material is 1: 0.2, adding while homogenizing and emulsifying, wherein the homogenizing and emulsifying is shearing homogenizing and emulsifying, the rotating speed of the shearing homogenizing and emulsifying is 10000rpm, and the time is 20min, until the system is uniform, a stable oil-in-water system is formed, free oil materials are not generated, and the uniformly-shaped nutritional paste is obtained;

s4, sterilization and packaging: sterilizing the nutritional paste at 70 deg.C for 15min, and packaging according to production requirement.

Example 3 the edible method of the nutritional paste is to eat instantly after uncovering, and about 1-5 g of the nutritional paste is taken every day according to the nutritional requirements.

Example 3 sensory indices of the nutritional cream are as follows:

color: the color is yellow brown or dark brown, and is uniform and consistent;

taste and smell: the flavor is strong, and the product can be melted in the mouth;

the form is as follows: the paste is semisolid, and the phenomenon of phase separation does not occur after the paste is placed for a long time;

impurities: no visible impurities.

Comparative example 1

An activated calcium solution for antarctic krill, which is different from the activated calcium solution in example 3, is characterized in that the step of adding the antarctic krill protein peptide solution to the antarctic krill calcium dilution is omitted in comparative example 1.

Test example 1

The 18 ICR mice were used as test animals, and were randomly divided into two groups by physical mass, i.e., example 3 group and comparative example 1 group. Fasting was for 10h before feeding. The same Ca was used for mice in both experimental groups²⁺The solutions of activated calcium from Antarctic krill prepared in step (3) S1 in example 3 and comparative example 1 were fed separately in a gavage volume of 10mL/kg x body weight. Then, feces discharged from the mice of each test group for 24 hours were collected, and the calcium content in the feces was measured. Calcium absorption was calculated as follows:

calcium absorption (%) (calcium content in gastric perfusion liquid-24 h calcium content in feces)/calcium content in gastric perfusion liquid x 100%.

The test results are shown in table 1 below.

Table 1 calcium absorption rates of the example 3 group and the comparative example 1 group

Item	Example 3	Comparative example 1
			Calcium absorption rate (%)	81.3	60.7

Test results show that the protein peptide solution can be added into the calcium diluent to remarkably improve the absorption rate of calcium.

Although the present invention has been described in detail by referring to the drawings in connection with the preferred embodiments, the present invention is not limited thereto. Various equivalent modifications or substitutions can be made on the embodiments of the present invention by those skilled in the art without departing from the spirit and scope of the present invention, and these modifications or substitutions are within the scope of the present invention/any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.