Pesticide composition containing fenpyroximate and application thereof
阅读说明：本技术 一种含唑螨酯的农药组合物及其应用 (Pesticide composition containing fenpyroximate and application thereof ) 是由 刘金玲 杨志鹏 张芳 王玉 于 2020-12-05 设计创作，主要内容包括：本发明属于农药技术领域,涉及一种含唑螨酯的农药组合物,所述农药组合物的有效成分包含活性成分A和活性成分B；所述活性成分A为唑螨酯,所述活性成分B为flupentiofenox。本发明的农药组合物或其制剂高效广谱,对若螨和成螨都有防治效果,具有较强的胃毒和触杀作用,兼具内吸传导作用；持效期长,在作物发生初期用药,能够有效控制作物整个生长期不受害螨的危害。(The invention belongs to the technical field of pesticides, and relates to a pesticide composition containing fenpyroximate, wherein the active ingredients of the pesticide composition comprise an active ingredient A and an active ingredient B; the active ingredient A is fenpyroximate, and the active ingredient B is flupentiofenox. The pesticide composition or the preparation thereof has high efficiency and broad spectrum, has control effect on nymph and adult mites, has strong stomach toxicity and contact killing effect, and has the effect of internal absorption and conduction; the pesticide has long lasting period, is applied at the initial stage of crop emergence, and can effectively control the whole growth period of crops from being damaged by mites.)
1. A pesticide composition containing fenpyroximate is characterized by comprising an active ingredient A of the fenpyroximate and an active ingredient B of flupentitionox.
2. The pesticide composition as claimed in claim 1, wherein the mass ratio of the active ingredient A to the active ingredient B is 1: 50-30: 1.
3. The pesticide composition as claimed in claim 1, wherein the mass ratio of the active ingredient A to the active ingredient B is 1: 30-15: 1.
4. A pesticidal composition according to any one of claims 1 to 3, characterised in that the sum of the amounts of active ingredient a and active ingredient B in the pesticidal composition is from 1 to 95 wt%, preferably from 5 to 80 wt%, more preferably from 10 to 50 wt%, based on 100 wt% of the total weight of the pesticidal composition.
5. The pesticidal composition of any one of claims 1-4, further comprising an adjuvant selected from one or more of a wetting agent, a dispersing agent, an emulsifier, a co-emulsifier, a thickener, a disintegrant, an antifreeze agent, an antifoaming agent, a solvent, a preservative, a stabilizer, a synergist, an anti-caking agent, a carrier, or a filler.
6. The pesticidal composition according to any one of claims 1 to 5, wherein the formulation of the pesticidal composition is selected from the group consisting of emulsifiable concentrates, aqueous emulsions, microemulsions, suspensions, wettable powders, water dispersible granules;
preferably, the formulation is in a form selected from the group consisting of an aqueous emulsion and a suspending agent.
7. Use of the pesticidal composition of any one of claims 1 to 5, the formulation of claim 6 for controlling plant pests.
8. Use of the formulation of claim 7 for controlling plant pests, said plants being selected from the group consisting of crops, fruit trees, vegetables;
preferably, the plant is Lycium barbarum L.
9. The use of a pesticidal composition according to any one of claims 7 to 8, wherein the pests on lycium barbarum are from the order homoptera, coleoptera, thysanoptera or acarina;
preferably, the pests on the medlar are from the order acarina cecidae: lycium barbarum gall mite (Acerimacroodonis Keifer), Lycium barbarum rust mite (Aculops lycii Kuang);
more preferably, the pest on the medlar is goiter of medlar.
Technical Field
The invention relates to the technical field of pesticide compounding, in particular to a pesticide composition containing fenpyroximate.
Background
Lycium plants belonging to the genus Lycium of the family Solanaceae are important medical and edible plant resources in China. The fructus Lycii is rich in crude protein, crude fat, carotenoid, thiamine, riboflavin, ascorbic acid, betaine, and various vitamins and amino acids. China is a world leading area of medlar, is well sold around the world and is popular with people all over the world. Because the medicinal value of the medlar is high, the economic benefit is high, and the planting scale and the industrialization level are rapidly improved. Along with the enlargement of the planting area of the medlar, the occurrence types and the occurrence range of the medlar mites tend to be enlarged year by year. The goiter of Chinese wolfberry is one of the most serious pests on Chinese wolfberry, the pest degree is serious, the control difficulty is high, and the yield of Chinese wolfberry is seriously influenced.
Fenpyroximate (fenpyroximate) is a pyrazole acaricide without systemic action. Has good quick action and effective period, and is effective on various spider mites and rust mites. Mitochondrial membrane electron transfer inhibitors on Complex I. Has contact poisoning and stomach poisoning activities, and has quick knockdown activity on larva, nymph and imago. The low concentration of fenpyroximate treats nymphs, which, although developable, inhibit their molting. CAS registry number 134098-61-6.
Flupentiofenox was developed by Nippon combinatorial chemistry industries, Inc., and was developed under the development code K II-9396. (RS) - [5- [ 4-chloro-2-fluoro-5- [ (2,2, 2-trifluoroethyl) sulfinyl group]Phenoxy radical]Pentyl radical]Trifluoromethyl sulfide; CAS accession numbers are: 1472050-04-6; the molecular formula is as follows: c14H14ClF7O2S2The chemical structural formula is as follows:
the fruit yield and the quality are reduced due to serious damage of the goiter of the medlar, and the chemical agent is the most effective means for preventing and treating the goiter of the medlar in the actual production. In addition, in order to realize green food production and reduce residues, chemical pesticides can be applied only in the growth period of the medlar for control, and chemical pesticides cannot be used in the mature period of fruits. Based on the problems, a pesticide composition which has the advantages of stomach toxicity, contact killing, systemic conduction, long lasting period and safety to crops and non-target organisms is urgently needed for preventing and controlling the goiter of the Chinese wolfberry. The compound composition of the fenpyroximate and the flupentiofenox has obvious synergistic effect, long duration and safety to crops and non-target organisms.
Disclosure of Invention
Based on the situation, the invention aims to provide a pesticide acaricidal composition containing fenpyroximate and a preparation thereof, which are mainly used for preventing and treating medlar mites.
In order to achieve the aim, the invention provides a pesticide composition containing fenpyroximate, which comprises an active ingredient A of fenpyroximate and an active ingredient B of flupentiophenox;
further, the mass ratio of the active component A to the active component B is 1: 50-30: 1;
further, the mass ratio of the active ingredient A to the active ingredient B is 1: 30-15: 1;
further, the mass ratio of the active component A to the active component B is 1:20, 1:15, 1:10, 1:5, 1:1, 5:1 and 15: 1;
further, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 1-95 wt%, preferably 5-80 wt%, and more preferably 10-50 wt%, based on 100 wt% of the total weight of the pesticide composition;
in a preferred embodiment, the sum of the amounts of fenpyroximate and flupentiofenox in the pesticidal composition is 10-50 wt%, for example, the sum of the amounts of fenpyroximate and flupentiofenox is 30%, 28%, 24%, 22%, 20% or 18%.
The invention optimizes the content of the effective components in the pesticide composition, so that the toxicity and the residue of the pesticide composition are well balanced, the pesticide effect can be enhanced, the dosage can be reduced, and the cost can be reduced.
Further, the pesticide composition also comprises an auxiliary agent, wherein the auxiliary agent is selected from one or more of a wetting agent, a dispersing agent, an emulsifying agent, a co-emulsifying agent, a thickening agent, a disintegrating agent, an antifreezing agent, an antifoaming agent, a solvent, a preservative, a stabilizing agent, a synergist, an anti-caking agent, a carrier or a filler;
furthermore, the pesticide composition has a dosage form selected from missible oil, aqueous emulsion, microemulsion, suspending agent, wettable powder and water dispersible granules;
further, the preparation is selected from aqueous emulsion and suspending agent;
an emulsion in water (EW) is a pesticide preparation formed by dispersing a raw drug liquid which is insoluble in water or a liquid obtained by dissolving a raw drug in an organic solvent which is insoluble in water. The basic formula of the aqueous emulsion comprises:
the solvent is selected from aromatic hydrocarbon compounds such as benzene, toluene, xylene and durene;
the emulsifier is selected from polyoxyethylene fatty alcohol, polyoxyethylene alkylphenol, alkyl polyglycol ether, alkyl phenyl polyglycol ether, polyoxyethylene sorbitan ester, polyoxyethylene fatty acid ester, etc.;
the coemulsifier is selected from straight-chain alkyl alcohols such as butanol, isobutanol, dodecanol, tetradecanol and octadecanol;
the thickener is selected from xanthan gum, polyvinyl alcohol, magnesium aluminum silicate, sodium alginate, guar gum, gelatin, etc.;
the antifreezing agent is selected from ethylene glycol, propylene glycol, glycerol, urea, sorbitol, ammonium sulfate, etc.;
the antiseptic is selected from sorbic acid, benzoic acid, benzaldehyde, etc.;
the defoaming agent is selected from organosilicone, C8-C10 fatty alcohol, C10-C20 saturated fatty acid, ester ether and the like;
adding the effective components, solvent, emulsifier and co-emulsifier together, stirring and dissolving to obtain uniform oil phase; mixing deionized water, an antifreezing agent, a preservative and the like into a water phase, and gradually adding the water phase into an oil phase under high-speed stirring to ensure that the system is slowly converted from a water-in-oil type into an oil-in-water type emulsion in water.
A suspension is a dosage form in a liquid formulation, but unlike a true solution, it is a flowable multiphase dispersion. Between colloidal dispersion and coarse dispersion, it is an unstable dispersion. The suspending agent consists of pesticide active ingredients, a dispersing agent, a wetting agent, a thickening agent, a stabilizing agent, a pH regulator, an antifreezing agent, a defoaming agent and the like. The suspending agent comprises the following basic formula:
the dispersant is selected from lignosulfonate (sodium lignosulfonate, calcium lignosulfonate), alkyl naphthalene sulfonate formaldehyde polycondensate, carboxylate high molecular polymer, EO-PO block copolymer, etc.;
the wetting agent is selected from sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, sodium dibutyl naphthalene sulfonate, dioctyl sodium sulfosuccinate, and the like;
the thickener is selected from xanthan gum, sodium carboxymethylcellulose, polyvinyl alcohol, magnesium aluminum silicate, sodium alginate, guar gum, etc.;
the antifreezing agent is selected from ethylene glycol, glycerol, polyethylene glycol, urea, sorbitol, etc.;
the stabilizer is selected from bentonite, white carbon black, light calcium carbonate and the like;
the defoaming agent is selected from organosilicone and C8～C10Fatty alcohol of (2), C10～C20Saturated fatty acids and ester ethers;
mixing the raw medicinal materials with dispersant, wetting agent, deionized water, etc. at a certain proportion, pre-dispersing, grinding in a sand mill, and pulverizing to powder D90(the grain diameter of 90 percent of grains) is less than 10 mu m, obtaining crushed slurry, mixing a thickening agent, a stabilizing agent, a defoaming agent, an antifreezing agent, deionized water and the like after the grain diameter requirement is met, and modulating to obtain the white homogeneous phase water suspending agent.
The pesticide composition obtained by compounding the effective components of the fenpyroximate and flupentiofenox has obvious synergistic effect, has strong control effect, and can effectively control plant pests;
furthermore, the plants are crops, fruit trees and vegetables;
further, the plant is Lycium barbarum L (Lycium bararum L.);
further, the pests on the medlar are homoptera, coleoptera, thysanoptera and acarina;
homoptera pests on common lycium barbarum: aphididae (aphids sp. lycii), psyllidae (Poratriozania grandiflora Yang et Li.); coleopteran pest phyllanthaceae (lycium barbarum epixabeille (bauduer), lycium barbarum l. schneid lemadempuncurtata Gebler); thrips of the order thysanoptera (thrips lycii pslothripsiindicus Bhatti); acarina order gall mite family (Lycium barbarum gall Acerimacroodonis Keifer, Lycium barbarum rust Acallops lycii Kuang).
Further, the pests on the medlar are from the order acarina cecidae: lycium barbarum gall mite (Acerimacroodonis Keifer), Lycium barbarum rust mite (Aculops lycii Kuang); further, the pest on the medlar is medlar gall mite.
The goiter of Chinese wolfberry is harmful to leaves, young shoots, petals, flower buds and young fruits, yellow green round raised goiter is formed after the harmful cells are stimulated, and the leaves and the young shoots are deformed and bent; the rust mites of the medlar are distributed on two sides of the main vein of the base part of the back of the leaf, the leaf is densely distributed in the colony body to suck juice, the leaf becomes thick, hard and bent downwards to be in a rusty brown stain, and the leaf becomes rusty color and early falls when the stain is serious.
In summary, compared with the prior art, the technical scheme of the invention has the following beneficial effects:
1) the acaricidal composition and the preparation thereof have high efficiency and broad spectrum, have strong stomach toxicity and contact killing effect, and have the effect of systemic conduction;
2) the pesticide has long lasting period, is applied at the initial stage of crop occurrence, and can effectively control the whole growth period of crops from being damaged by mites;
3) is safe to crops, non-target organisms, beneficial organisms and natural enemies.
Detailed Description
To make the technical solutions, objects, and advantages of the present invention more apparent, the present invention is described with the following specific examples, but the present invention may be implemented in various forms and should not be limited by the embodiments set forth herein.
Indoor toxicity assay
95% of flupentiofenox technical material and 96% of fenpyroximate technical material are provided by group research and development centers.
Test targets: goiter of Chinese wolfberry.
The toxicity of the adult mites is measured by a Potter spraying method. Dissolving raw medicines with acetone to prepare a mother solution, designing 5 groups of mixture ratios according to mixing purposes and medicament activities, and diluting the mother solution with 0.1% Tween 80 aqueous solution by using each single agent and each group of mixture ratios according to an equal ratio method for 5 series of mass concentrations. Picking up healthy newly emerged adult mites, placing the healthy newly emerged adult mites on the front surfaces of fresh wolfberry leaves with the same size and wrapped with wet absorbent cotton at the periphery, picking up 15 heads of the adult mites on each leaf, observing survival conditions of the mites after mite bodies are stabilized (about 30min), removing dead mites, and supplementing new adult mites. In the test, the leaves sprayed with clean water are used as a blank control and a solvent control is set. Each treatment was repeated 4 times, with 15 mites per repetition. Uniformly spraying liquid medicines with different concentrations on the front surface of the Chinese wolfberry leaves in a laboratory Potter spray tower. The initial spraying volume is 1mL, and the spraying time is 1min as standard parameters. And (3) wrapping 1 layer of absorbent cotton around the treated wolfberry leaves, and feeding in an incubator at the temperature of 25 +/-1 ℃, the relative humidity of 70-80% and the photoperiod L: D of 14:10 h.
If the toxicity of the mites is measured by a leaf soaking method. Dissolving raw medicines with acetone to prepare mother liquor, designing 5 groups of mixture ratios according to mixing purposes and medicament activities, and diluting the mother liquor by 0.1% Tween 80 aqueous solution for 5 series of mass concentrations by using each single agent and each group of mixture ratios according to an equal ratio method. Immersing fresh and clean Chinese wolfberry leaves into the prepared series of liquid medicines for 10s, wrapping 1 layer of absorbent cotton around the dried leaves, and placing the leaves in a culture dish paved with wet sponge. 15 mites were inoculated on each leaf, and after the mite bodies stabilized (about 30min), the number of mites was checked under binocular dissection glasses and the deficient mites were supplemented. And (3) putting the treated medlar leaves into an incubator at the temperature of 25 +/-1 ℃, the relative humidity of 70-80% and the photoperiod L: D of 14:10h for feeding. In the test, the leaves treated with clear water are used as a blank control, and a solvent control is set. Each treatment was repeated 4 times, each time 15 d nymphs.
After treatment (adult mites 24h, nymph 48h) the death of the test mites was checked, and the death of adult mites was checked under binocular dissection. The mites were touched with a brush tip, and were considered dead if they were not sufficient, and the total number of the insects and the number of the dead insects were recorded separately.
From the survey data, corrected mortality for each treatment was calculated. Calculating according to the formulas (1) and (2), and reserving the calculation results to the last two decimal points:
in the formula:
p-mortality in percent (%);
k-represents the number of dead mites in head;
n-represents the total number of insects treated, in heads.
In the formula:
P1corrected mortality in percent (%);
Pt-treatment mortality in percent (%);
P0blank mortality in percent (%).
And processing the data by adopting a method of analyzing a few rate value. LC for solving virulence regression line by using IBM SPSS Statistics 2.0 statistical analysis system for analysis50The value and its 95% confidence limit, the activity of the test agent on the biological test material is evaluated.
The co-toxicity coefficient (CTC value) of the mixture is calculated according to the following formula (3), formula (4) and formula (5):
in the formula:
ATI-actually measured toxicity index of mixed agent;
S-LC of Standard acaricide50In milligrams per liter (mg/L);
LC of M-mixtures50In milligrams per liter (mg/L).
TTI＝TIA*PA+TIB*PB·······(4)
In the formula:
TTI-mixture theory virulence index;
TIA-agent virulence index a;
PA-the percentage of agent a in the mixture in percent (%);
TIB-agent B virulence index;
PB-the percentage of the agent B in the mixture in percent (%).
In the formula:
CTC-co-toxicity coefficient;
ATI-actually measured toxicity index of mixed agent;
TTI-mixture theory virulence index.
The compounded co-toxicity coefficient CTC is more than or equal to 120, and the synergistic effect is shown; CTC is less than or equal to 80 and shows antagonism; 80 < CTC < 120 showed additive effects.
Example 1 mite-formation virulence assay
Combined toxicity determination of fenpyroximate and flupentiofenox on Lycium chinense gall mite (adult mite) in different ratios
As can be seen from table 1: the toxicity of the fenpyroximate and flupentiofenox to the goiter lycii is greatly different between different proportions. Fenpyroximate has good acaricidal activity on adult mites, LC50A value of 0.222mg/L, flupentiofenox versus adult mite LC50The value was 0.778 mg/L. The mixing in the proportion tested in the test shows additive or synergistic effect on the adult mites; fenpyroximate: flupentiofenox is 1:10, and has the best effect on mite formation, and LC50The value is 0.417mg/L, the co-toxicity coefficient is 151.970, and the synergistic effect is shown.
TABLE 1 Combined virulence determination of goiter lycii (acarus barbarum) by different proportions of fenpyroximate and flupentiofenox
Example 2 Dermatophagoides virulence assay
Combined toxicity determination of fenpyroximate and flupentiofenox on Lycium chinense gall mite (nymph mite) in different ratios
As can be seen from table 2: the toxicity of fenpyroximate and flupentiofenox to Lycium barbarum gall mite is greatly different between different proportions. Fenpyroximate has good acaricidal activity to nymphs, LC50A value of 0.036mg/L, flupentiofenox vs. nymphs LC50The value was 0.175 mg/L. The tested proportion of the mixed pair of the acarids in the test is 1: 50-30: 1, and the acarids are added or synergistic, wherein the control effect is 1: 15-15: 1, and the acarids are synergistic; fenpyroximate: flupentiofenox-1: 5 is the most effective against nymphs, LC50The value is 0.064mg/L, the co-toxicity coefficient is 166.373, and the synergistic effect is shown.
TABLE 2 Combined virulence determination of goiter barbarum (Rumex) by different ratios of fenpyroximate and flupentiofenox
The fenpyroximate and flupentiofenox are mixed for use, so that the acaricide has high-efficiency broad-spectrum acaricidal activity, the dosage can be reduced, the service life of the compound can be prolonged, and the pesticide residue can be reduced. However, ideal control effects can not be achieved by blending different acaricides, tests show that toxicity of the fenpyroximate and flupentiofenox for goiter lycii is measured and an optimal formula is screened by researching the mixed pairing of the fenpyroximate and the flupentiofenox, and results show that the fenpyroximate and the flupentiofenox are 1:10 for adult mites and 1:5 for nymphs as the optimal formula, so that theoretical support is provided for reasonably blending the acaricides.
Preparation example 1: 22% fenpyroximate flupentiofenox emulsion in water (1:10)
Adding fenpyroximate raw drug, 20 flupentiofenox raw drug, 2% polyoxyethylene sorbitan ester, 1% alkyl phenyl polyglycol ether and 0.5% dodecanol together, and stirring to dissolve into uniform oil phase; deionized water, 0.5 percent of xanthan gum, 3 percent of propylene glycol, 0.1 percent of benzoic acid, 0.1 percent of organic silicon and the like are mixed into a water phase, the water phase is gradually added into an oil phase under high-speed stirring, and the system is slowly changed from water-in-oil type to oil-in-water type 22 percent fenpyroximate flupentiofenox aqueous emulsion.
Preparation example 2: 24% fenpyroximate flupentiofenox emulsion in water (1:5)
Adding 4% fenpyroximate technical product, 20% flupentiofenox technical product, 3% alkyl phenyl polyglycol ether and 1% isobutanol, stirring and dissolving to form uniform oil phase; mixing deionized water, 0.5% xanthan gum, 3% glycerol, 0.1% sorbic acid and 0.1% organosilicon into a water phase, and gradually adding the water phase into an oil phase under high-speed stirring to slowly convert the water-in-oil type system into the oil-in-water type 24% fenpyroximate flupentiofenox water emulsion.
Preparation example 3: 18% fenpyroximate flupentiofenox emulsion in water (1:8)
Adding 2% fenpyroximate technical product, 16% flupentiofenox technical product, 2.5% alkyl phenyl polyglycol ether and 1.5% isobutanol, stirring and dissolving into uniform oil phase; mixing deionized water, 0.5% of polyvinyl alcohol, 4% of ethylene glycol, 0.1% of sorbic acid, 0.1% of organic silicon and the like to form a water phase, and gradually adding the water phase into an oil phase under high-speed stirring to slowly convert the water-in-oil type system into the oil-in-water type 18% fenpyroximate flupentitofenox water emulsion.
Preparation example 4: 24% fenpyroximate flupentiofenox suspending agent (1:5)
3% of sodium lignosulfonate and 3% of sodium dodecyl benzene sulfonate are dispersed in deionized water, then 20% of flupentiofenox technical and 4% of fenpyroximate technical are added, and wet grinding is carried out on the mixture by a sand mill by using glass beads until D90 (the particle size of 90% of particles) is less than 10 mu m, so as to obtain the grinding slurry. Adding 2% xanthan gum into the crushed slurry,5% of glycerol, 0.5% of white carbon black and 3% of C10～C20And (3) uniformly mixing saturated fatty acids, complementing the deionized water to 100%, and uniformly shearing at a high speed to obtain the fenpyroximate flupentiofenox suspending agent with the mass content of the effective component of 24%.
Preparation example 5: 20% fenpyroximate flupentiofenox suspending agent (1:9)
Dispersing 2% of carboxylate high-molecular polymer and 2.5% of dioctyl sodium sulfosuccinate in deionized water, adding 18% of flupentiofenox technical and 2% of fenpyroximate technical, and performing wet grinding on the mixture by using glass beads and a sand mill to obtain D90 (the particle size of 90% of particles) is less than 10 mu m, thereby obtaining the ground slurry. Adding 2% of sodium carboxymethylcellulose, 5% of urea, 0.5% of light calcium carbonate and 0.5% of organic silicone into the crushed slurry, uniformly mixing, complementing deionized water to 100%, and uniformly shearing at high speed to obtain the fenpyroximate flupentiofenox suspending agent with the mass content of the effective component of 20%.
Preparation example 6: 28% fenpyroximate flupentiofenox suspending agent (1:6)
3% of sodium lignosulfonate and 3% of sodium dodecyl benzene sulfonate are dispersed in deionized water, then 24% of flupentiofenox technical and 4% of fenpyroximate technical are added, and wet grinding is carried out on the mixture by a sand mill by using glass beads until D90 (the particle size of 90% of particles) is less than 10 mu m, so as to obtain the grinding slurry. Adding 2% xanthan gum, 5% ethylene glycol, 1.5% light calcium carbonate and 3% C into the pulverized slurry10～C20And (3) uniformly mixing saturated fatty acids, complementing the deionized water to 100%, and uniformly shearing at a high speed to obtain the fenpyroximate flupentiofenox suspending agent with the mass content of the effective component of 28%.
Preparation example 7: 30% fenpyroximate flupentiofenox suspending agent (1:14)
Dispersing 3% of carboxylate high-molecular polymer and 2.5% of sodium dibutylnaphthalenesulfonate in deionized water, adding 28% of flupentiofenox technical and 2% of fenpyroximate technical, and performing wet grinding on the mixture by using glass beads and a sand mill to obtain D90 (the particle size of 90% of particles) is less than 10 mu m to obtain ground slurry. Adding 2.5% of magnesium aluminum silicate, 6% of glycerol, 0.5% of bentonite and 3% of C into the crushed slurry10～C20Saturated fatty acids and mixingUniformly adding deionized water to 100%, and uniformly shearing at high speed to obtain the fenpyroximate flupentiofenox suspending agent with the effective component mass content of 30%.
The above are all weight percentages.
Example 2 control of goiter of Lycium barbarum in field test
Test work: fructus Lycii (medlar) Ningqi I;
test subjects: gall mites.
Test and control agents:
TABLE 3 test, control and dosage
The test is carried out in a Chinese wolfberry plantation with higher Chinese wolfberry gall mite damage degree in late 5 months, 230 Chinese wolfberry plants are planted per mu, the tree ages are 5 years, and the tree vigor is consistent. The soil fertility of the test field is medium, the terrain is smooth, the fertility is uniform, and the irrigation condition is good. All test plot cultivation conditions (soil type, fertilization, cultivation, row spacing, etc.) were uniform and consistent with local agricultural cultivation practices (GAP). The preparation treatment, the control treatment and the blank control adopt random block arrangement, 5 lycium barbarum trees are randomly selected for spraying in each treatment, the spraying is repeated for 4 times, the control spraying is carried out firstly when the pesticide is applied, the spraying is carried out from low concentration to high concentration in sequence, and the spraying is uniformly carried out at constant speed according to the calculated step speed by adopting a constant spraying method. When different medicaments are replaced, the sprayer is cleaned for three times, and water in the spray rod is completely sprayed out.
The weather during the test period is good, and on the test medicine day, the daily average temperature is 20 ℃, the highest temperature is 27 ℃, the lowest temperature is 19 ℃ and the relative humidity is 70%.
The investigation method comprises the following steps: 2 of 5 plants are extracted from each pre-treatment, 1 current-year branch is randomly selected from 5 directions of east, west, south, north and middle of each plant, is marked by hanging, and the occurrence degree of the mite damage on 25 leaves at the tip of each branch is investigated according to the grading standard of grade 9 mite damage. The mite damage indexes are investigated at 7d, 14d and 21d after the pesticide respectively, and the control effect is calculated.
Investigating insect condition indexes in 9 grades:
normal leaf of grade 0;
level 1: 1-2 insect gall plaques with the size less than 1 mm;
and 3, level: 2-3 insect gall plaques with the sizes larger than 1mm and smaller than 2 mm;
and 5, stage: there are more than 3 galls below 2 mm;
and 7, stage: galls with a size of more than 2 mm;
and 9, stage: has teratogenic leaves or twigs.
The drug effect calculation method comprises the following steps: the drug effect is calculated by the following formula (6) and formula (7):
the test data is statistically analyzed by adopting IBM SPSS Statistics 2.0 software, and the difference of the field control effect is remarkably analyzed by adopting a Duncan's new range method.
Test results of the treatment agents for controlling goiter of medlar:
TABLE 4 post-dose 7d field test results
Note: the control (%) in the table above is the average of each repetition. The lower case letters represent 5% level difference and the upper case letters represent 1% level difference (table 5, table 6, the same).
TABLE 5 post-dose 14d field test results
TABLE 6 post-dose 21d field test results
As can be seen from the results of field experiments of different test agents on Lycium barbarum gall mites in tables 4-6, the acaricide composition obtained by compounding fenpyroximate and flupentiofenox has better control effect on Lycium barbarum gall mites. After the pesticide is applied for 7 days, a compounded pesticide of 22% fenpyroximate flupentiofenox aqueous emulsion (1:10), 24% fenpyroximate flupentiofenox aqueous emulsion (1:5), 24% fenpyroximate flupentiofenox suspending agent (1:5) and 20% fenpyroximate flupentiofenox suspending agent (1:9) respectively have control effects of 86.48%, 85.86%, 85.43% and 81.04%, and under the levels of 0.01 and 0.05, the three control effects of 22% fenpyroximate flupentiofenox aqueous emulsion (1:10), 24% fenpyroximate flupentiofenox aqueous emulsion (1:5) and 24% fenpyroximate flupentiofenox suspending agent (1:5) do not have mixing difference. 14 days after the pesticide is applied, the control effects of 22% fenpyroximate flupentiofenox emulsion in water (1:10), 24% fenpyroximate flupentiofenox emulsion in water (1:5), 24% fenpyroximate flupentiofenox suspending agent (1:5) and 20% fenpyroximate flupentiofenox suspending agent (1:9) are 90.97%, 88.36%, 86.88% and 88.78% respectively, and the control effects of the control medicament 20% flupentiofenox suspending agent and 5% fenpyroximate suspending agent are 83.09% and 85.33% respectively. After 21 days, the control effect of each compound pesticide is more than 90%, the 22% fenpyroximate flupentiofenox aqueous emulsion (1:10) and the 20% fenpyroximate flupentiofenox suspending agent (1:9) are respectively 92.33% and 92.94%, and each pesticide shows better lasting effect, so that the acaricidal composition and the preparation thereof prepared by compounding are remarkable in control effect.
Through indoor toxicity measurement and experiments in field medlar, the pesticide composition compounded by the fenpyroximate and the flupentiofenox has good control effect on phytophagous harmful mites. The pesticide composition compounded by fenpyroximate and flupentiofenox is used for treating gall mites, and the pesticide composition has a good control effect on adult mites and nymphs. The pesticide composition has long lasting period, and is safe to crops in order to find that the compound pesticide generates phytotoxicity to the crops in the test.
The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention accordingly, and not to limit the protection scope of the present invention accordingly. All equivalent changes or modifications made in accordance with the spirit of the present disclosure are intended to be covered by the scope of the present disclosure.