阅读说明：本技术 促进双孢蘑菇菌丝生长及产量提高的微量元素复合溶液 (Microelement complex solution for promoting agaricus bisporus hypha growth and improving yield ) 是由 王倩 黄建春 肖婷婷 隽加香 张津京 陈辉 宋晓霞 陈明杰 于 2020-09-30 设计创作，主要内容包括：本发明主要提供一种促进双孢蘑菇菌丝生长及产量提高的微量元素复合溶液,用于至少一双孢蘑菇,所述促进双孢蘑菇菌丝生长及产量提高的微量元素复合溶液包括锌元素Zn、铜元素Cu、锰元素Mn、镁元素Mg、铁元素Fe、以及钙元素Ca,从而促进所述双孢蘑菇的菌丝生长和产量提升。本发明通过对所述双孢蘑菇的培养基中的微量元素的成分和相应的含量进行添加和控制,从而促进双孢蘑菇菌丝生长及产量提升。(The invention mainly provides a microelement complex solution for promoting the growth and yield improvement of agaricus bisporus hyphae, which is used for at least agaricus bisporus, and the microelement complex solution for promoting the growth and yield improvement of the agaricus bisporus hyphae comprises zinc element Zn, copper element Cu, manganese element Mn, magnesium element Mg, iron element Fe and calcium element Ca, so that the growth and yield improvement of the agaricus bisporus hyphae is promoted. According to the invention, the components and corresponding contents of the trace elements in the culture medium of the agaricus bisporus are added and controlled, so that the growth of agaricus bisporus hyphae and the yield increase are promoted.)

1. A microelement complex solution for promoting the growth and yield improvement of agaricus bisporus hyphae is used for at least agaricus bisporus and is characterized in that the microelement complex solution for promoting the growth and yield improvement of the agaricus bisporus hyphae comprises zinc element Zn, copper element Cu, manganese element Mn, magnesium element Mg, iron element Fe and calcium element Ca, so that the growth and yield improvement of the agaricus bisporus hyphae is promoted.

2. The trace element complex solution for promoting hypha growth and yield improvement of agaricus bisporus according to claim 1, wherein the agaricus bisporus is a model a 15.

3. The complex solution of trace elements for promoting the growth of mycelia and increasing the yield of Agaricus bisporus according to claim 2, wherein the content of Zn is 0.0001mM, the content of Cu is 0.001mM, the content of Mn is 0.001mM, the content of Mg is 0.5mM, the content of Fe is 0.001mM, and the content of Ca is 0.05 mM.

4. The complex solution of trace elements for promoting the growth and production of mycelia of Agaricus bisporus according to claim 3, wherein the complex solution of trace elements for promoting the growth and production of mycelia of Agaricus bisporus is applied to a medium of Agaricus bisporus when the Agaricus bisporus is in a material-sowing state or when the germination of the Agaricus bisporus is completed.

5. The trace element complex solution for promoting the growth and yield of agaricus bisporus mycelium according to claim 3, wherein the trace element complex solution for promoting the growth and yield of agaricus bisporus mycelium is applied to casing soil when water is sprayed in a mushroom house after the agaricus bisporus is in the casing soil.

6. The microelement complex solution for promoting the hypha growth and the yield improvement of the agaricus bisporus according to claim 5, wherein the zinc element Zn increases the yield of the agaricus bisporus by not less than 20.4%.

7. The microelement complex solution for promoting the hypha growth and yield increase of the agaricus bisporus according to claim 5 or 6, wherein the manganese element Mn increases the yield of the agaricus bisporus by not less than 31.9%.

8. The microelement complex solution for promoting the hypha growth and yield increase of the agaricus bisporus according to claim 7, wherein the iron element Fe increases the yield of the agaricus bisporus by not less than 22.7%.

9. The microelement complex solution for promoting the growth and yield increase of agaricus bisporus hyphae according to claim 5 or 8, wherein the magnesium element Mg increases the yield of agaricus bisporus by not less than 11.1%.

10. The microelement complex solution for promoting the hypha growth and the yield improvement of the agaricus bisporus according to claim 1, wherein the agaricus bisporus is W192.

11. The complex solution of trace elements for promoting the growth of mycelia and increasing the yield of Agaricus bisporus according to claim 10, wherein the content of Zn is 0.0001mM, the content of Cu is 0.001mM, the content of Mn is 0.001mM, the content of Mg is 0.5mM, the content of Fe is 0.1mM, and the content of Ca is 0.1 mM.

12. The complex solution of trace elements for promoting the growth and production of mycelia of Agaricus bisporus according to claim 11, wherein the complex solution of trace elements for promoting the growth and production of mycelia of Agaricus bisporus is applied to a medium of Agaricus bisporus when the Agaricus bisporus is in a material-sowing state or when the germination of the Agaricus bisporus is completed.

13. The microelement complex solution for promoting the growth and yield increase of the hypha of the agaricus bisporus according to claim 12, wherein the yield increase amplitude achieved by applying the microelement complex solution for promoting the growth and yield increase of the hypha of the agaricus bisporus during the loading and sowing of the agaricus bisporus is 10.7% -18.2%.

14. The microelement complex solution for promoting the growth and yield increase of the hyphae of the agaricus bisporus according to claim 12, wherein the yield increase amplitude achieved by applying the microelement complex solution for promoting the growth and yield increase of the hyphae of the agaricus bisporus when the agaricus bisporus is spawned is completed is 1.8% -15.3%.

15. The trace element complex solution for promoting the growth and yield of agaricus bisporus mycelium according to claim 11, wherein the trace element complex solution for promoting the growth and yield of agaricus bisporus mycelium is applied to casing soil when water is sprayed at a mushroom house after the agaricus bisporus is in the casing soil.

16. The complex microelement solution for promoting the growth and yield increase of agaricus bisporus mycelium according to claim 15, wherein the zinc element Zn increases the yield of agaricus bisporus by not less than 5.6%.

17. The complex microelement solution for promoting the growth and yield increase of agaricus bisporus mycelium according to claim 15 or 16, wherein the manganese element Mn increases the yield of agaricus bisporus by not less than 13.6%.

18. The microelement complex solution for promoting the hypha growth and yield increase of the agaricus bisporus according to claim 17, wherein the iron element Fe increases the yield of the agaricus bisporus by not less than 10.7%.

19. A microelement complex solution for promoting the growth of agaricus bisporus hyphae and the yield increase according to claim 15 or 18, wherein the magnesium element Mg increases the yield of agaricus bisporus by not less than 4.0%.

Technical Field

The invention relates to the field of biology, in particular to a trace element compound solution capable of promoting growth of agaricus bisporus hyphae and improving yield.

Background

The agaricus bisporus is a rotten edible fungus which is most widely artificially cultivated, has the highest total output and the largest consumption in the world, is also a mushroom which has a larger cultivation area and is most imported by exports in China, is rich in various amino acids, vitamins and the like, is rich in nutrition and is delicious in taste.

Along with the development of science and technology, the living standard of people is continuously improved, the demand of the market for edible fungi is increasingly large, the edible requirements of people on edible fungi can not be met by the traditional mode of manually cultivating the edible fungi, and the industrial cultivation of the edible fungi becomes a necessary trend. At present, the yield of factory-cultivated agaricus bisporus in China is 22-28kg/m²30-35kg/m more abroad²There is still much room for improvement in yield. Under the condition that cultivation raw materials and cultivation technology are not changed, the improvement of the substrate biotransformation efficiency of the culture material is an important means for improving the mushroom yield. .

In addition, agaricus bisporus is saprophytic fungus, cannot perform photosynthesis, and needs to grow and develop by absorbing nutrients existing in a culture medium. And carbon, nitrogen, minerals and trace elements are indispensable nutrient components for the growth and development of the agaricus bisporus. The carbon in the agaricus bisporus decomposition culture medium mainly depends on a lignocellulose enzyme system, comprising cellulase, hemicellulase and ligninase, and the nitrogen in the decomposition culture medium mainly depends on protease. The activity of the key enzymes for carbon and nitrogen metabolism is a key factor influencing the substrate biotransformation efficiency, and further determines the yield of the agaricus bisporus. The key enzymes playing main roles in different stages in the growth and development process of agaricus bisporus are different, and are detailed in the following table.

The trace elements are components of the active center of the microorganism, and are activators of many enzymes. The trace elements are added in different growth and development stages of the agaricus bisporus in a targeted manner, so that the method is beneficial to regulating and controlling key enzymes in the growth and development process of the agaricus bisporus, and the substrate conversion efficiency is improved, thereby playing roles in increasing the yield of the agaricus bisporus and improving the quality of the agaricus bisporus.

Disclosure of Invention

One advantage of the present invention is to provide a trace element complex solution for promoting the growth and yield of agaricus bisporus hyphae, which can promote the growth and yield of agaricus bisporus hyphae of a type a15, a type W192, and the like.

One advantage of the present invention is to provide a trace element complex solution for promoting the growth and yield of agaricus bisporus mycelium, which promotes the growth and yield increase of agaricus bisporus mycelium by controlling the components of trace elements in a culture medium of agaricus bisporus.

One advantage of the present invention is to provide a trace element complex solution for promoting the growth and yield of agaricus bisporus mycelium, which promotes the growth and yield increase of agaricus bisporus mycelium by controlling the content of trace elements in a culture medium of agaricus bisporus.

One advantage of the present invention is to provide a trace element composite solution for promoting the growth and yield of agaricus bisporus hyphae, wherein the trace element composite solution for promoting the growth and yield of agaricus bisporus hyphae is prepared by adding trace elements to agaricus bisporus in different cultivation stages, so as to promote the growth and yield of agaricus bisporus hyphae.

One advantage of the present invention is to provide a trace element composite solution for promoting growth and yield improvement of agaricus bisporus hyphae, wherein the trace element composite solution for promoting growth and yield improvement of agaricus bisporus hyphae provides different trace element components for different types of agaricus bisporus, so as to promote growth and yield improvement of agaricus bisporus hyphae of different types.

One advantage of the present invention is to provide a trace element complex solution for promoting the growth and yield of agaricus bisporus hyphae, wherein the trace element complex solution for promoting the growth and yield of agaricus bisporus hyphae is added in different stages of the agaricus bisporus hyphae growth in a targeted manner, so that the activity of key enzymes is improved, and the substrate biotransformation rate is improved.

One advantage of the present invention is to provide a trace element complex solution for promoting the growth and yield of agaricus bisporus hyphae, which is added to a compost at a hypha growth stage (a seeding stage or a spawn running stage) to promote the enzymatic activity of a key enzyme at the hypha growth stage.

One advantage of the present invention is to provide a trace element composite solution for promoting the growth and yield of agaricus bisporus hyphae, wherein the trace element composite solution for promoting the growth and yield of agaricus bisporus hyphae is added in the covering soil and spraying water at the growth and development stage of sporocarp, including the spraying water at the needle stage and the spraying water at the harvest stage at the cooling and bud forcing stage, so as to promote the enzymatic activity of key enzymes.

In order to achieve at least the advantages of the invention, the invention provides a trace element composite solution for promoting the growth and yield improvement of agaricus bisporus hyphae, which is used for at least agaricus bisporus, and the trace element composite solution for promoting the growth and yield improvement of the agaricus bisporus hyphae comprises zinc element Zn, copper element Cu, manganese element Mn, magnesium element Mg, iron element Fe and calcium element Ca, so that the growth and yield improvement of the agaricus bisporus hyphae is promoted.

In some of these embodiments, the agaricus bisporus is a model a 15.

In some of these examples, the content of Zn element is 0.0001mM, the content of Cu element is 0.001mM, the content of Mn element is 0.001mM, the content of Mg element is 0.5mM, the content of Fe element is 0.001mM, and the content of Ca element is 0.05 mM.

In some embodiments, the microelement complex solution for promoting the growth of agaricus bisporus hyphae and improving the yield is applied to the culture medium of the agaricus bisporus when the agaricus bisporus is in the material sowing or when the fungus growing is completed.

In some embodiments, the trace amount of the complex solution for promoting the growth of mycelia and increasing the yield of agaricus bisporus is applied to the casing while spraying water at a mushroom house after the agaricus bisporus is in the casing.

In some of these embodiments, the zinc element Zn increases the yield of the agaricus bisporus by not less than 20.4%.

In some of these embodiments, the manganese element Mn increases the yield of the agaricus bisporus by not less than 31.9%.

In some of these embodiments, the iron element Fe increases the yield of the agaricus bisporus by not less than 22.7%.

In some of these embodiments, the magnesium element Mg increases the yield of the agaricus bisporus by not less than 11.1%.

In some of these embodiments, the agaricus bisporus is type W192.

In some of these examples, the content of Zn element is 0.0001mM, the content of Cu element is 0.001mM, the content of Mn element is 0.001mM, the content of Mg element is 0.5mM, the content of Fe element is 0.1mM, and the content of Ca element is 0.1 mM.

In some embodiments, the microelement complex solution for promoting the growth of agaricus bisporus hyphae and improving the yield is applied to the culture medium of the agaricus bisporus when the agaricus bisporus is in the material sowing or when the fungus growing is completed.

In some embodiments, the yield increase amplitude achieved by applying the microelement complex solution for promoting the growth and yield increase of agaricus bisporus hyphae in the process of feeding and sowing the agaricus bisporus is 10.7-18.2%.

In some embodiments, the trace element compound solution for promoting the growth of agaricus bisporus hyphae and improving the yield is applied when the agaricus bisporus spawn running is finished, so that the yield increase amplitude is 1.8-15.3%.

In some embodiments, the trace amount of the complex solution for promoting the growth of mycelia and increasing the yield of agaricus bisporus is applied to the casing while spraying water at a mushroom house after the agaricus bisporus is in the casing.

In some of these embodiments, the zinc element Zn increases the yield of the agaricus bisporus by not less than 5.6%.

In some of these embodiments, the manganese element Mn increases the yield of the agaricus bisporus by not less than 13.6%.

In some of these embodiments, the Fe element increases the yield of agaricus bisporus by not less than 10.7%.

In some of these embodiments, the magnesium element Mg increases the yield of the agaricus bisporus by not less than 4.0%.

Drawings

FIG. 1 is a graph showing the relationship between each element and the growth rate of Agaricus bisporus mycelium when the microelement complex solution for promoting the growth of the Agaricus bisporus mycelium and improving the yield of the Agaricus bisporus is applied to the type A15 Agaricus bisporus.

FIG. 2 is a graph showing the yield increase ratio of Agaricus bisporus model A15 under the action of Zn, Mn, Fe and Mg.

Fig. 3 shows fruiting conditions of a first embodiment of the microelement complex solution for promoting hypha growth and yield improvement of agaricus bisporus in an orthogonal test.

FIG. 4 is a graph showing the relationship between each element and the growth rate of Agaricus bisporus mycelium when the microelement complex solution for promoting the growth of Agaricus bisporus mycelium and improving the yield of the Agaricus bisporus is applied to a type W192 Agaricus bisporus.

FIG. 5 is a schematic diagram showing the yield increase ratio of the type W192 Agaricus bisporus under the action of Zn, Mn, Fe and Mg.

Fig. 6 is experimental data of the microelement complex solution for promoting the hypha growth and yield improvement of the agaricus bisporus of the type W192 applied to a culture medium at a feeding and sowing stage of the agaricus bisporus.

Fig. 7 is experimental data of the microelement complex solution for promoting the hypha growth and yield improvement of the agaricus bisporus of the type W192 applied to a culture medium at the stage of completion of spawn running of the agaricus bisporus.

Detailed Description

The following description is presented to disclose the invention so as to enable any person skilled in the art to practice the invention. The preferred embodiments in the following description are given by way of example only, and other obvious variations will occur to those skilled in the art. The basic principles of the invention, as defined in the following description, may be applied to other embodiments, variations, modifications, equivalents, and other technical solutions without departing from the spirit and scope of the invention.

It is understood that the terms "a" and "an" should be interpreted as meaning that a number of one element or element is one in one embodiment, while a number of other elements is one in another embodiment, and the terms "a" and "an" should not be interpreted as limiting the number.

The invention mainly provides a microelement composite solution for promoting the growth and yield improvement of agaricus bisporus hyphae, which is used for providing microelement nutrition for at least agaricus bisporus, and the microelement composite solution for promoting the growth and yield improvement of the agaricus bisporus hyphae at least comprises zinc element Zn, copper element Cu, manganese element Mn, magnesium element Mg, iron element Fe and calcium element Ca, so that the microelement composite solution for promoting the growth and yield improvement of the agaricus bisporus hyphae can promote the growth and yield improvement of the agaricus bisporus hyphae.

In the first embodiment of the composite solution of trace elements for promoting the growth and yield of agaricus bisporus hyphae, the composite solution of trace elements for promoting the growth and yield of agaricus bisporus hyphae is used in agaricus bisporus model a15, and the composite solution of trace elements for promoting the growth and yield of agaricus bisporus hyphae comprises zinc element Zn, copper element Cu, manganese element Mn, magnesium element Mg, iron element Fe, and calcium element Ca, wherein the content of each element is 0.0001mM for zinc element Zn, 0.001mM for copper element Cu, 0.001mM for manganese element Mn, 0.5mM for magnesium element Mg, 0.001mM for iron element Fe, and 0.05mM for calcium element Ca.

As shown in fig. 1, the relationship between Zn, Cu, Mn, Mg, Fe, Ca and the growth rate of agaricus bisporus hyphae in the complex solution of trace elements for promoting the growth of agaricus bisporus hyphae and improving the yield when the complex solution is applied to agaricus bisporus type a15 is shown.

The trace element compound solution for promoting the growth of the hyphae of the agaricus bisporus and improving the yield can be added in any step of applying trace elements to the agaricus bisporus in the cultivation stage, and the hyphae growth and the yield of the agaricus bisporus can be improved.

Preferably, in the first embodiment of the microelement complex solution for promoting the growth and yield improvement of the agaricus bisporus hyphae, the microelement complex solution for promoting the growth and yield improvement of the agaricus bisporus hyphae is selected to be applied on the covering soil when the agaricus bisporus is sown by the material loading (namely, secondary material), when the spawn running is finished (namely, tertiary material) and when the mushroom house sprays water after the covering soil, so that the promotion effect on the hypha growth and yield improvement of the agaricus bisporus is improved.

Next, experiments were carried out on agaricus bisporus model a15 to which the trace element complex solution of the present invention for promoting the growth of agaricus bisporus hyphae and increasing the yield was applied at different stages and corresponding results were obtained. Namely, the effect of the microelement complex solution for promoting the hypha growth and the yield improvement of the agaricus bisporus of A15 model when the agaricus bisporus of A15 model is applied after soil covering.

The microelement complex solution for promoting the growth of the agaricus bisporus hyphae and improving the yield is applied to the soil covering when a mushroom house sprays water after the agaricus bisporus is covered with the soil, and in the step after the agaricus bisporus is applied to the soil covering, different single-factor experiments are carried out on the microelement complex solution for promoting the growth of the agaricus bisporus hyphae and improving the yield, namely the microelement complex solution for promoting the growth of the agaricus bisporus hyphae and improving the yield is implemented into different components, so that the influence effect of the microelement complex solution containing different components for promoting the growth of the agaricus bisporus hyphae and improving the yield on the yield of the agaricus bisporus when the microelement complex solution acts on the agaricus bisporus with the A15 model is observed.

According to experiments, the microelement complex solution containing different components and capable of promoting the growth and the yield of the agaricus bisporus hyphae can improve the hypha growth and the yield of the agaricus bisporus model A15 to different degrees. The most obvious effect is Zn, Mn, Fe and Mg, as shown in FIG. 2, the yield of A15 type Agaricus bisporus is respectively increased by 20.4%, 31.9%, 22.7% and 11.1% under the action of Zn, Mn, Fe and Mg.

In addition, in the process of applying the experiment on the agaricus bisporus of model A15 by the microelement complex solution for promoting the growth of the agaricus bisporus hyphae and improving the yield, in order to determine whether the sporocarp has an enrichment effect on microelements with different components, particularly heavy metals, the sporocarp which is treated differently is collected and tested, so that the content of each microelement in the sporocarp is obtained.

The measured trace element content in the fruiting body of the agaricus bisporus, model a15, is shown in table one below.

Watch 1

As can be seen from the above table, under the experimental conditions, the fruiting bodies subjected to different treatments have no enrichment effect on the applied trace elements, so that the trace element composite solution for promoting the growth of agaricus bisporus hyphae and improving the yield does not cause safety problems in the use process.

In addition, in order to obtain the influence of the trace elements with different components in the trace element compound solution for promoting the growth of the agaricus bisporus hyphae and improving the yield on the yield of the agaricus bisporus model A15, an orthogonal test was further carried out.

As shown in the following table two, firstly, zinc element Zn, manganese element Mn, iron element Fe and magnesium element Mg which perform better in the single-factor test are selected, and a four-factor three-level orthogonal test is performed on the agaricus bisporus strain of the a15 model, and the result is shown in the table two.

Note: CK yield was 32.5kg/m²

Watch two

As can be seen from the second table above, the treatment achieved a yield increase effect on the A15 type Agaricus bisporus except for the 8 and 9 treatments, and the highest yield of the A15 type Agaricus bisporus was 42.1kg/m²An increase of 29.5% compared to the control yield.

Fig. 3 shows fruiting conditions of a first embodiment of the microelement complex solution for promoting hypha growth and yield improvement of agaricus bisporus in an orthogonal test. As can be seen from fig. 3 and table two, in the orthogonal test of the agaricus bisporus model a15, the primary and secondary sequences of the functions of each trace element are zinc element Zn, iron element Fe, manganese element Mn and magnesium element Mg, and the optimal content combinations of the zinc element Zn, the iron element Fe, the manganese element Mn and the magnesium element Mg in the test are 0.001mM of zinc element Zn, 0.001mM of iron element Fe, 0.0001mM of manganese element Mn and 0.1mM of magnesium element Mg, respectively.

As shown in FIG. 1, the fruiting condition of the agaricus bisporus of A15 model in the orthogonal test is shown.

Further, as shown in table three, the results of the cross-over test were subjected to anova, and it can be seen from the anova that the P values of Zn and Fe were < 0.001, and thus the effect on the yield of the a15 type agaricus bisporus was very significant, indicating that these two trace elements (i.e., Zn and Fe) were major elements in the present experiment, while Mn and Mg were minor elements.

Watch III

Next, in a second embodiment of the present invention, the present invention provides a composite solution of trace elements for promoting growth and yield of agaricus bisporus hyphae, wherein the composite solution of trace elements for promoting growth and yield of agaricus bisporus hyphae is used in W192 type agaricus bisporus, and the composite solution of trace elements for promoting growth and yield of agaricus bisporus hyphae comprises Zn, Cu, Mn, Mg, Fe, and Ca, wherein the content of each element is 0.0001mM for Zn, 0.001mM for Cu, 0.001mM for Mn, 0.5mM for Mg, 0.1mM for Fe, and 0.1mM for Ca.

As shown in fig. 4, the relationship between Zn, Cu, Mn, Mg, Fe, Ca and the growth rate of agaricus bisporus mycelium in the microelement complex solution for promoting agaricus bisporus mycelium growth and yield improvement of the present invention when applied to W192 type agaricus bisporus is shown.

The trace element compound solution for promoting the growth of the hyphae of the agaricus bisporus and improving the yield can be added in any step of applying trace elements to the agaricus bisporus in the cultivation stage, and the hyphae growth and the yield of the agaricus bisporus can be improved.

Preferably, in the second embodiment of the composite solution of trace elements for promoting the growth and yield of agaricus bisporus hyphae of the present invention, the composite solution of trace elements for promoting the growth and yield of agaricus bisporus hyphae is selected to be applied on the covering soil when the agaricus bisporus is sown (i.e., secondary material), when the spawn running is completed (i.e., tertiary material), and when the mushroom house is sprayed with water after the covering soil, so as to improve the promotion effect on the growth and yield improvement of agaricus bisporus hyphae.

Next, experiments were carried out on W192 type agaricus bisporus in different stages to which the trace element complex solution of the present invention for promoting the growth of agaricus bisporus hyphae and increasing the yield was applied, and corresponding results were obtained. Namely, when the microelement complex solution for promoting the hypha growth and the yield improvement of the agaricus bisporus is applied after the agaricus bisporus of the W192 model is covered with soil, the influence on the agaricus bisporus of the W192 model is caused.

The microelement complex solution for promoting the growth of the agaricus bisporus hyphae and improving the yield is applied to the soil covering when a mushroom house sprays water after the agaricus bisporus is covered with the soil, and in the step after the soil covering, different single-factor experiments are carried out on the microelement complex solution for promoting the growth of the agaricus bisporus hyphae and improving the yield, namely the microelement complex solution for promoting the growth of the agaricus bisporus hyphae and improving the yield is implemented into different components, so that the effect of the microelement complex solution containing different components for promoting the growth of the agaricus bisporus hyphae and improving the yield on the yield of the agaricus bisporus when the microelement complex solution acts on the agaricus bisporus with the W192 model is observed.

According to experiments, the microelement complex solution containing different components and capable of promoting the growth and the yield of the agaricus bisporus hyphae can improve the hypha growth and the yield of the agaricus bisporus of the W192 type to different degrees. The most obvious effect is Zn, Mn, Fe and Mg, as shown in FIG. 5, the yield of the type W192 agaricus bisporus is respectively increased by 5.6%, 13.6%, 10.7% and 4.0% under the action of Zn, Mn, Fe and Mg.

In addition, in the process of applying experiments on the agaricus bisporus of the W192 type through the microelement complex solution for promoting the hypha growth and the yield improvement of the agaricus bisporus, in order to determine whether the sporocarp has an enrichment effect on microelements with different components, particularly heavy metals, the sporocarp which is treated differently is collected and tested, so that the content of each microelement in the sporocarp is obtained.

The contents of trace elements tested in the fruiting body of the agaricus bisporus of type W192 are shown in table four below.

Watch four

As can be seen from the above table, under the experimental conditions, the fruiting bodies subjected to different treatments have no enrichment effect on the applied trace elements, so that the trace element composite solution for promoting the growth of agaricus bisporus hyphae and improving the yield does not cause safety problems in the use process.

Next, the second example of the microelement complex solution for promoting the growth of agaricus bisporus hyphae and improving the yield of agaricus bisporus is further tested and verified.

The trace element compound solution for promoting the growth of agaricus bisporus hyphae and improving the yield is applied in the steps of material sowing (secondary material) and fungus growing (tertiary material) of the agaricus bisporus of W192 type respectively. Because the agaricus bisporus is implemented in the culture medium when the agaricus bisporus is fed and sown and when the agaricus bisporus is spawned, the microelement complex solution for promoting the agaricus bisporus hypha growth and improving the yield can be added into the culture medium and evenly stirred with the nutrients in the culture medium.

As shown in fig. 6, there are experimental data on the culture medium to which the complex solution of trace elements for promoting the growth of mycelia and increasing the yield of agaricus bisporus according to the present invention is applied when the type W192 agaricus bisporus is in the stage of the material-loading sowing.

As shown in fig. 7, the trace element complex solution for promoting the growth of agaricus bisporus hyphae and increasing the yield of agaricus bisporus according to the present invention was applied to the culture medium at the stage of completion of germination of the agaricus bisporus type W192.

In both FIGS. 6 and 7, the yield was counted by using a basket sprayed with the same amount of tap water as a Control (CK).

As can be seen from fig. 6 and 7, the trace element complex solution for promoting the growth of agaricus bisporus hyphae and improving the yield is applied to the agaricus bisporus of W192 type in the secondary material and the fermentation material, so that the agaricus bisporus of W192 type has a yield increase effect.

Wherein, except zinc element Zn, the yield increase amplitude after other trace elements are applied in the secondary material is 10.7-18.2%; in the fungus growing material, the trace elements with the effect of increasing the yield comprise zinc element Zn, copper element Cu, manganese element Mn, iron element Fe and magnesium element Mg, and the zinc element Zn, the copper element Cu, the manganese element Mn, the iron element Fe and the magnesium element Mg provide the yield increase amplitude of 1.8-15.3 for the agaricus bisporus with the W192 model.

In addition, based on the above disclosure of the present invention, those skilled in the art can adjust the components and/or contents of specific trace elements contained in the trace element complex solution for promoting the growth and yield of agaricus bisporus hyphae of the present invention, as long as the same or similar technical solution as the present invention is adopted, the same or similar technical problems as the present invention are solved, and the same or similar technical effects as the present invention are achieved, which all fall within the protection scope of the present invention, and the specific embodiment of the present invention is not limited thereto.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

It will be appreciated by persons skilled in the art that the embodiments of the invention shown in the foregoing description are by way of example only and are not limiting of the invention. The objects of the invention have been fully and effectively accomplished. The functional and structural principles of the present invention have been shown and described in the examples, and any variations or modifications of the embodiments of the present invention may be made without departing from the principles.