阅读说明：本技术 一种三苯基乙酸锡与戊唑醇的杀菌组合物及其应用 (Bactericidal composition of triphenyltin acetate and tebuconazole and application thereof ) 是由 张青华 王进 徐瑶 李雪芬 潘光飞 陈华 金晓恩 唐银东 曾挺 于 2020-12-17 设计创作，主要内容包括：本发明公开了一种三苯基乙酸锡与戊唑醇的杀菌组合物及其应用,杀菌组合物的活性成分为三苯基乙酸锡与戊唑醇,其中三苯基乙酸锡与戊唑醇重量比为1:10～10:1。所述杀菌组合物以三苯基乙酸锡与戊唑醇为活性成分,其中三苯基乙酸锡与戊唑醇重量比为1:10～10:1。该杀菌组合物将三苯基乙酸锡与戊唑醇复配使用,具有很好的协同增效杀菌作用,本发明杀菌组合物扩大了杀菌防治谱,提高了杀菌效率,避免了病害的抗药性,延长了药物持效期,该杀菌组合物在防治蔬菜、果树病害上的应用,特别是在防治蔬菜、果树黑星病、斑点落叶病、晚疫病、纹枯病、赤霉等病害中有显著的杀菌防治效果。(The invention discloses a triphenyltin acetate and tebuconazole bactericidal composition and application thereof, wherein active ingredients of the bactericidal composition are triphenyltin acetate and tebuconazole, and the weight ratio of the triphenyltin acetate to the tebuconazole is 1: 10-10: 1. The bactericidal composition takes triphenyl tin acetate and tebuconazole as active ingredients, wherein the weight ratio of the triphenyl tin acetate to the tebuconazole is 1: 10-10: 1. The sterilization composition has the advantages that the triphenyltin acetate and the tebuconazole are compounded for use, the sterilization composition has good synergistic sterilization effect, the sterilization control spectrum is expanded, the sterilization efficiency is improved, the drug resistance of diseases is avoided, the drug duration is prolonged, the sterilization composition is applied to the control of the diseases of vegetables and fruit trees, and particularly has remarkable sterilization control effect in the control of the diseases of vegetables and fruit trees, such as scab, alternaria leaf spot, late blight, banded sclerotial blight, gibberellic disease and the like.)

1. The bactericidal composition containing the triphenyltin acetate and the tebuconazole is characterized in that active ingredients of the bactericidal composition are the triphenyltin acetate and the tebuconazole, wherein the weight ratio of the triphenyltin acetate to the tebuconazole is 1: 10-10: 1.

2. The bactericidal composition of triphenyltin acetate and tebuconazole according to claim 1, wherein the active ingredients of the bactericidal composition are triphenyltin acetate and tebuconazole, and the weight ratio of the triphenyltin acetate to the tebuconazole is 1: 1-5: 1.

3. The bactericidal composition containing triphenyltin acetate and tebuconazole according to claim 1, wherein the dosage form of the bactericidal composition containing triphenyltin acetate and tebuconazole is wettable powder or dispersible oil suspending agent.

4. The bactericidal composition containing the triphenyltin acetate and the tebuconazole according to any one of claims 1 to 3, wherein the dosage form of the bactericidal composition is wettable powder and comprises the following components in percentage by weight: 1-80% of triphenyltin acetate and tebuconazole, 1-10% of dispersing agent, 1-10% of wetting agent and the balance of filler.

5. The bactericidal composition of triphenyltin acetate and tebuconazole according to claim 4, wherein the dosage form of the bactericidal composition is wettable powder and comprises the following components in percentage by weight: 30-70% of triphenyl tin acetate and tebuconazole, 4-8% of dispersing agent, 1-3% of wetting agent and the balance of filler.

6. The bactericidal composition of triphenyltin acetate and tebuconazole according to claim 4, wherein the dispersing agent is formed by mixing sodium naphthalene sulfonate and sodium lignosulfonate in a mass ratio of 2: 1; the wetting agent is formed by mixing sodium dodecyl sulfate and sodium lignin sulfonate according to the mass ratio of 1: 1; the filler is formed by mixing 20% of white carbon black and 80% of talcum powder.

7. The bactericidal composition of triphenyltin acetate and tebuconazole according to any one of claims 1 to 3, wherein the dosage form of the bactericidal composition is a dispersed oil suspending agent, takes an organic solvent as a medium, and consists of the following components in percentage by weight: 1-60% of triphenyltin acetate and tebuconazole, 0.5-20% of wetting agent, 0.2-20% of dispersing agent, 0.5-20% of emulsifying agent, 0-10% of suspending aid and the balance of dispersing medium.

8. The bactericidal composition of triphenyltin acetate and tebuconazole according to any one of claims 1 to 3, wherein the dosage form of the bactericidal composition is a dispersed oil suspending agent, takes an organic solvent as a medium, and consists of the following components in percentage by weight: 20-50% of triphenyltin acetate and tebuconazole, 1-5% of wetting agent, 2-8% of dispersing agent, 2-8% of emulsifying agent, 1-5% of suspension auxiliary agent and the balance of dispersing medium.

9. The bactericidal composition of triphenyltin acetate and tebuconazole according to claim 7, wherein the dispersing agent is formed by mixing an alkylphenol ethoxylate-formaldehyde condensate with fatty alcohol-polyoxyethylene ether sulfate according to the mass ratio of 1: 1; the wetting agent is sodium dodecyl sulfate; the emulsifier is formed by mixing calcium dodecyl benzene sulfonate and castor oil polyoxyethylene ether according to the mass ratio of 1: 1; the suspension auxiliary agent is formed by mixing white carbon black and organic bentonite according to the mass ratio of 1: 1; the dispersion medium is methyl oleate.

10. The application of the bactericidal composition of triphenyltin acetate and tebuconazole according to any one of claims 1 to 9 in prevention and treatment of scab, alternaria leaf spot, late blight, downy mildew, rust disease, powdery mildew, banded sclerotial blight and gibberellic disease of vegetables and fruit trees.

Technical Field

The invention relates to the field of pesticide compounding, and particularly relates to a bactericidal composition of triphenyltin acetate and tebuconazole and application thereof.

Background

Triphenyltin acetate, formula: c₂₀H₁₈O₂Sn is a non-systemic fungicide which can be absorbed by roots, stems and leaves and has an inhibiting effect on diseases caused by phycomycetes, oomycetes and the like. Has better protection and treatment effects on bacterial diseases, can effectively prevent and treat some fungi which are sensitive to copper bactericides, and has better prevention effect on the brown spot of beet.

Tebuconazole, chemical name 1- (4-chlorophenyl) -4, 4-dimethyl-3 (1H-1,2, 4-triazol-1-ylmethyl) pentan-3-ol, formula: c₁₆H₂₂ClN₃And O. Tebuconazole belongs to triazole bactericides and is a high-efficiency systemic bactericide used for seed treatment or leaf surface spraying of important economic crops. The compound with hydroxyl (keto group), substituted phenyl and 1,2, 4-triazole group in main chain of tebuconazole can inhibit the biosynthesis of ergot catalpol in fungi and inhibit the membrane lipid fluidity and stable molecules of pathogenic bacteria, thereby damaging the membrane structure and function and causing the death of thallus cells. The tebuconazole bactericide has the characteristics of wide bactericidal spectrum, high activity, high bactericidal speed, long lasting period, strong systemic conductivity and the like, and is one of typical representative varieties of triazole bactericides. Can be used for preventing and treating diseases caused by powdery mildew, puccinia, coracocephalum, pyrenophora and septoria of cereal crops. Can effectively prevent and control wheat loose smut, stinking smut and the like.

At present, the application of the triphenyltin acetate and tebuconazole which are compounded for use in preventing and treating scab, alternaria leaf spot, late blight, downy mildew, rust disease, powdery mildew, banded sclerotial blight and gibberellic disease of vegetables and fruit trees is not reported. Therefore, the triphenyltin acetate and the tebuconazole are combined and compounded for use, so that the bactericidal composition containing the triphenyltin acetate and the tebuconazole is developed, has high-efficiency broad-spectrum bactericidal property, and has good effects on preventing and treating diseases such as powdery mildew, cladosporium cucumerinum, alternaria leaf spot and the like of crops.

Disclosure of Invention

Aiming at the problems that a bactericide with a single active ingredient can generate drug resistance and low bactericidal property after being repeatedly used for a long time, the invention provides a compound bactericidal composition containing triphenyltin acetate and tebuconazole with synergistic bactericidal action.

In view of the above, the present invention provides a bactericidal composition containing triphenyltin acetate and tebuconazole and an application thereof, so as to solve the above technical problems.

The active ingredients of the sterilization composition are triphenyl tin acetate and tebuconazole, wherein the weight ratio of the triphenyl tin acetate to the tebuconazole is 1: 10-10: 1.

Preferably, the active ingredients of the bactericidal composition are triphenyl tin acetate and tebuconazole, wherein the weight ratio of the triphenyl tin acetate to the tebuconazole is 1: 1-5: 1.

Preferably, the dosage form of the bactericidal composition containing the triphenyltin acetate and the tebuconazole is wettable powder or dispersible oil suspending agent.

Preferably, the dosage form of the bactericidal composition is wettable powder and consists of the following components in percentage by weight: 1-80% of triphenyltin acetate and tebuconazole, 1-10% of dispersing agent, 1-10% of wetting agent and the balance of filler.

More preferably, the formulation of the bactericidal composition is wettable powder, and the bactericidal composition is composed of the following components in percentage by weight: 30-70% of triphenyl tin acetate and tebuconazole, 4-8% of dispersing agent, 1-3% of wetting agent and the balance of filler.

More preferably, the dispersing agent is formed by mixing sodium naphthalene sulfonate and sodium lignin sulfonate according to the mass ratio of 2: 1; the wetting agent is formed by mixing sodium dodecyl sulfate and sodium lignin sulfonate according to the mass ratio of 1: 1; the filler is formed by mixing 20% of white carbon black and 80% of talcum powder.

Preferably, the formulation of the bactericidal composition is a dispersed oil suspending agent, an organic solvent is used as a medium, and the bactericidal composition comprises, by weight, 1% -60% of triphenyltin acetate and tebuconazole, 0.5-20% of a wetting agent, 0.2-20% of a dispersing agent, 0.5-20% of an emulsifying agent, 0-10% of a suspending aid and the balance of a dispersed medium.

More preferably, the formulation of the bactericidal composition is a dispersed oil suspending agent, takes an organic solvent as a medium, and consists of the following components in percentage by weight: 20-50% of triphenyltin acetate and tebuconazole, 1-5% of wetting agent, 2-8% of dispersing agent, 2-8% of emulsifying agent, 1-5% of suspension auxiliary agent and the balance of dispersing medium.

More preferably, the dispersing agent is formed by mixing an alkylphenol polyoxyethylene ether formaldehyde condensate and fatty alcohol polyoxyethylene ether sulfate according to the mass ratio of 1: 1; the wetting agent is sodium dodecyl sulfate; the emulsifier is formed by mixing calcium dodecyl benzene sulfonate and castor oil polyoxyethylene ether according to the mass ratio of 1: 1; the suspension auxiliary agent is formed by mixing white carbon black and organic bentonite according to the mass ratio of 1: 1; the dispersion medium is methyl oleate.

The sterilization composition of the triphenyltin acetate and the tebuconazole is mainly applied to preventing and treating diseases such as scab, alternaria leaf spot, late blight, downy mildew, rust disease, powdery mildew, banded sclerotial blight, gibberellic disease and the like of vegetables and fruit trees. Preferably, the first and second electrodes are formed of a metal,

according to the technical scheme, the invention has the beneficial effects that:

compared with a single triphenyl tin acetate or tebuconazole bactericide, the sterilization composition prepared by the invention combines triphenyl tin acetate and tebuconazole for use, has good synergistic sterilization effect, expands the sterilization control spectrum, improves the sterilization efficiency, avoids the drug resistance of diseases, prolongs the drug duration, and has obvious sterilization control effect in controlling the diseases of vegetables and fruit trees, in particular in controlling the diseases of vegetables and fruit trees such as scab, alternaria leaf spot, late blight, banded sclerotial blight, gibberellic disease and the like.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. The exemplary embodiments and descriptions of the present invention are provided to explain the present invention, but not to limit the present invention.

The triphenyltin acetate and the tebuconazole are calculated according to the fold percentage.

In the bactericidal composition wettable powder containing the triphenyl tin acetate and the tebuconazole, a dispersant is formed by mixing sodium naphthalene sulfonate and sodium lignosulfonate according to the mass ratio of 2: 1; the wetting agent is formed by mixing sodium dodecyl sulfate and sodium lignosulphonate according to the mass ratio of 1: 1; the filler is formed by mixing 20 percent of white carbon black and 80 percent of talcum powder.

In the bactericidal composition dispersing oil suspending agent containing the triphenyl tin acetate and the tebuconazole, the dispersing agent is formed by mixing an alkylphenol polyoxyethylene formaldehyde condensate and fatty alcohol polyoxyethylene ether sulfate according to the mass ratio of 1: 1; the wetting agent is sodium dodecyl sulfate; the emulsifier is formed by mixing calcium dodecyl benzene sulfonate and castor oil polyoxyethylene ether according to the mass ratio of 1: 1; the suspension auxiliary agent is formed by mixing white carbon black and organic bentonite according to the mass ratio of 1: 1; the dispersion medium is methyl oleate.

Example 1

Bactericidal composition of triphenyltin acetate and tebuconazole and application thereof

The bactericidal composition wettable powder containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 1g of triphenyl tin acetate, 10g of tebuconazole, 1g of dispersing agent and 1g of wetting agent, wherein the filling material is added to 100 g;

the preparation process of the wettable powder comprises the following steps: the components are primarily ground according to the mass, then added into a mixer to be uniformly mixed, then added into a free grinder to be mixed for 20min, finally ground and mixed for 25min through a jet mill, and sieved through a 300-mesh sieve, and the bactericidal composition wettable powder of 11% of triphenyltin acetate and tebuconazole is obtained.

Example 2

The bactericidal composition wettable powder containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 10g of triphenyl tin acetate, 1g of tebuconazole, 2g of dispersing agent and 3g of wetting agent, wherein the filling material is added to 100 g; the preparation process of the wettable powder is the same as that of the embodiment 1, and the 11 percent wettable powder of the sterilization composition of the triphenyltin acetate and the tebuconazole

Example 3

Bactericidal composition of triphenyltin acetate and tebuconazole and application thereof

The bactericidal composition wettable powder containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 5g of triphenyl tin acetate, 20g of tebuconazole, 3g of dispersing agent, 2g of wetting agent and 100g of filler. The preparation process of the wettable powder is the same as that of the embodiment 1, and the 25 percent wettable powder of the bactericidal composition of the triphenyltin acetate and the tebuconazole is obtained.

Example 4

Bactericidal composition of triphenyltin acetate and tebuconazole and application thereof

The bactericidal composition wettable powder containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 5g of triphenyl tin acetate, 20g of tebuconazole, 3g of dispersing agent, 2g of wetting agent and 100g of filler. The preparation process of the wettable powder is the same as that of the embodiment 1, and the 25 percent wettable powder of the bactericidal composition of the triphenyltin acetate and the tebuconazole is obtained.

Example 5

Bactericidal composition of triphenyltin acetate and tebuconazole and application thereof

The bactericidal composition wettable powder containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 10g of triphenyl tin acetate, 20g of tebuconazole, 2g of dispersing agent, 3g of wetting agent and 100g of filler. The preparation process of the wettable powder is the same as that of the embodiment 1, and the 30 percent wettable powder of the sterilization composition of the triphenyltin acetate and the tebuconazole is obtained.

Example 6

The bactericidal composition wettable powder containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 15g of triphenyl tin acetate, 20g of tebuconazole, 8g of dispersing agent and 8g of wetting agent, and the filler is added to 100 g. The preparation process of the wettable powder is the same as that of the embodiment 1, and the 35 percent wettable powder of the sterilization composition of the triphenyltin acetate and the tebuconazole is obtained.

Example 7

The bactericidal composition wettable powder containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 15g of triphenyl tin acetate, 30g of tebuconazole, 6g of dispersing agent, 7g of wetting agent and 100g of filler. The preparation process of the wettable powder is the same as that of the example 1, and the 45 percent wettable powder of the sterilization composition of the triphenyltin acetate and the tebuconazole is obtained.

Example 8

The bactericidal composition wettable powder containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 15g of triphenyl tin acetate, 30g of tebuconazole, 3g of dispersing agent, 2g of wetting agent and 100g of filler. The preparation process of the wettable powder is the same as that of the example 1, and the 45 percent wettable powder of the sterilization composition of the triphenyltin acetate and the tebuconazole is obtained.

Example 9

The bactericidal composition wettable powder containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 10g of triphenyl tin acetate, 40g of tebuconazole, 7g of dispersing agent, 4g of wetting agent and 100g of filler. The preparation process of the wettable powder is the same as that of the embodiment 1, and the 50 percent wettable powder of the sterilization composition of the triphenyltin acetate and the tebuconazole is obtained.

Example 10

The bactericidal composition wettable powder containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 35g of triphenyl tin acetate, 30g of tebuconazole, 4g of dispersing agent and 5g of wetting agent, and the filler is added to 100 g. The preparation process of the wettable powder is the same as that of the example 1, and the 65 percent wettable powder of the sterilization composition of the triphenyl tin acetate and the tebuconazole is obtained.

Example 11

The bactericidal composition wettable powder containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 45g of triphenyl tin acetate, 25g of tebuconazole, 6g of dispersing agent, 3g of wetting agent and 100g of filler. The preparation process of the wettable powder is the same as that of the embodiment 1, and the 70 percent wettable powder of the sterilization composition of the triphenyltin acetate and the tebuconazole is obtained.

Example 12

The bactericidal composition wettable powder containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 60g of triphenyl tin acetate, 20g of tebuconazole, 5g of dispersing agent, 2g of wetting agent and 100g of filler. The preparation process of the wettable powder is the same as that of the embodiment 1, and the 80 percent wettable powder of the bactericidal composition of the triphenyltin acetate and the tebuconazole is obtained.

Example 13

The bactericidal composition wettable powder containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 60g of triphenyl tin acetate, 20g of tebuconazole, 3g of dispersing agent, 2g of wetting agent and 100g of filler. The preparation process of the wettable powder is the same as that of the embodiment 1, and the 80 percent wettable powder of the bactericidal composition of the triphenyltin acetate and the tebuconazole is obtained.

Example 14

Bactericidal composition of triphenyltin acetate and tebuconazole and application thereof

The bactericidal composition dispersed oil suspending agent containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 5g of triphenyltin acetate, 10g of tebuconazole, 0.5g of wetting agent, 0.2g of dispersing agent, 0.5g of emulsifying agent and 100g of dispersing medium are added and mixed to prepare the 15 percent of bactericidal composition dispersed oil suspending agent of the triphenyltin acetate and the tebuconazole.

Example 15

Bactericidal composition of triphenyltin acetate and tebuconazole and application thereof

The bactericidal composition dispersed oil suspending agent containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 18g of triphenyltin acetate, 12g of tebuconazole, 2g of wetting agent, 3g of dispersing agent, 4g of emulsifying agent, 0.5g of suspension aid and 100g of dispersing medium are added and mixed to prepare the 20 percent of bactericidal composition dispersed oil suspending agent of the triphenyltin acetate and the tebuconazole.

Example 16

Bactericidal composition of triphenyltin acetate and tebuconazole and application thereof

The bactericidal composition dispersed oil suspending agent containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 20g of triphenyltin acetate, 5g of tebuconazole, 3.5g of wetting agent, 11g of dispersing agent, 8g of emulsifying agent, 1.5g of suspending aid and 100g of dispersing medium are added and mixed to prepare the 25 percent of bactericidal composition dispersed oil suspending agent of the triphenyltin acetate and the tebuconazole.

Example 17

Bactericidal composition of triphenyltin acetate and tebuconazole and application thereof

The bactericidal composition dispersed oil suspending agent containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 20g of triphenyltin acetate, 10g of tebuconazole, 10g of wetting agent, 7g of dispersing agent, 13g of emulsifying agent, 5g of suspension aid and 100g of dispersing medium, and mixing to obtain the 30% of bactericidal composition dispersed oil suspending agent of the triphenyltin acetate and the tebuconazole.

Example 18

Bactericidal composition of triphenyltin acetate and tebuconazole and application thereof

The bactericidal composition dispersed oil suspending agent containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 25g of triphenyltin acetate, 10g of tebuconazole, 12g of wetting agent, 12g of dispersing agent, 15g of emulsifying agent, 2g of suspending aid and 100g of dispersing medium, and mixing to obtain the 35% of bactericidal composition dispersed oil suspending agent of the triphenyltin acetate and the tebuconazole.

Example 19

Bactericidal composition of triphenyltin acetate and tebuconazole and application thereof

The bactericidal composition dispersed oil suspending agent containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 15g of triphenyltin acetate, 25g of tebuconazole, 4.5g of wetting agent, 13g of dispersing agent, 6.5g of emulsifying agent, 4g of suspending aid and 100g of dispersing medium are added and mixed to prepare the 40% of bactericidal composition dispersed oil suspending agent of the triphenyltin acetate and the tebuconazole.

Example 20

Bactericidal composition of triphenyltin acetate and tebuconazole and application thereof

The bactericidal composition dispersed oil suspending agent containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 30g of triphenyltin acetate, 15g of tebuconazole, 5g of wetting agent, 6g of dispersing agent, 9g of emulsifying agent, 3g of suspending aid and 100g of dispersing medium, and mixing to obtain the 45% of bactericidal composition dispersed oil suspending agent of the triphenyltin acetate and the tebuconazole.

Example 21

Bactericidal composition of triphenyltin acetate and tebuconazole and application thereof

The bactericidal composition dispersed oil suspending agent containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: adding 25g of triphenyltin acetate, 25g of tebuconazole, 6g of wetting agent, 0.5g of wetting agent, 0.2g of dispersing agent and 0.5g of emulsifying agent into 100g of dispersing medium, and mixing to obtain the 50% of bactericidal composition dispersed oil suspending agent of the triphenyltin acetate and the tebuconazole.

Example 22

Bactericidal composition of triphenyltin acetate and tebuconazole and application thereof

The bactericidal composition dispersed oil suspending agent containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 50g of triphenyltin acetate, 5g of tebuconazole, 5g of wetting agent, 0.5g of wetting agent, 0.2g of dispersing agent, 0.5g of emulsifying agent and 100g of dispersing medium are added and mixed to prepare the 55 percent of bactericidal composition dispersed oil suspending agent of the triphenyltin acetate and the tebuconazole.

Example 23

Bactericidal composition of triphenyltin acetate and tebuconazole and application thereof

The bactericidal composition dispersed oil suspending agent containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 40g of triphenyltin acetate, 20g of tebuconazole, 5g of wetting agent, 10g of dispersing agent, 10g of emulsifier, 2g of suspending aid and 100g of dispersing medium, and mixing to obtain the 60% of bactericidal composition dispersed oil suspending agent of the triphenyltin acetate and the tebuconazole.

Example 24

Bactericidal composition of triphenyltin acetate and tebuconazole and application thereof

The bactericidal composition dispersed oil suspending agent containing the triphenyltin acetate and the tebuconazole consists of the following components in parts by mass: 40g of triphenyltin acetate, 20g of tebuconazole, 12g of wetting agent, 12g of dispersing agent, 15g of emulsifying agent, 2g of suspending aid and 100g of dispersing medium are added and mixed to prepare the 60% of bactericidal composition dispersed oil suspending agent of the triphenyltin acetate and the tebuconazole.

Comparative example 1

The difference from example 6 is that the triphenyltin acetate is removed.

Comparative example 2

The difference from example 21 is that tebuconazole is removed.

Test examples

In order to determine the performance of the bactericidal composition of triphenyltin acetate and tebuconazole prepared by the invention, the bactericidal composition of triphenyltin acetate and tebuconazole prepared in the example and the bactericidal compositions of triphenyltin acetate and tebuconazole prepared in comparative examples 1 and 2 are respectively subjected to performance tests:

1. toxicity performance test of bactericidal composition containing triphenyltin acetate and tebuconazole

Method for testing toxicity of bactericidal composition containing triphenyl tin acetate and tebuconazole according to 'GB 15670-1995 pesticide registration toxicology test method', acute oral toxicity test, acute transdermal toxicity test, acute skin irritation test, eye irritation test and test dosage, test mouse case histology examination is carried out.

The results of the tests performed in examples 4 and 21 of the present invention are as follows:

(1) the average value of the bactericidal composition containing the triphenyltin acetate and the tebuconazole to the acute oral LD50 of adult female rats and adult male rats is more than 2985 mg/kg;

(2) the average value of the bactericidal composition containing the triphenyltin acetate and the tebuconazole to the acute percutaneous LD50 of both adult female rats and adult male rats is more than 2200 mg/kg;

(3) the average integral of the bactericidal composition containing the triphenyl tin acetate and the tebuconazole on the skin and eye irritation reaction of the rabbits is 0, and the scores of the skin irritation strengths are 0;

(4) under the condition of the tested dosage, the experimental results of bone marrow cell nucleus and sinwan chromosome aberration of the tested adult female rat and adult male rat are negative, so that the bactericidal composition containing the triphenyl tin acetate and the tebuconazole is proved to be toxic, non-irritant, weak sensitizer and free of mutation.

2. Indoor bioassay test for bactericidal composition containing triphenyltin acetate and tebuconazole

The method is characterized in that wheat sharp eyespot is taken as a test strain indoors, and the inhibition medium concentration EC of different agents on the strain is determined by adopting a hypha growth rate method₅₀Calculating the co-toxicity coefficient (CTC) of the mixture by adopting a Sun Yunpei co-toxicity coefficient calculation method, and determining the synergy of the mixture, wherein the calculation method comprises the following steps:

(1) using a single dosage of the mixture as a standard medicament (EC is usually selected)₅₀Lower), the calculation is performed:

(2) single dose virulence index (standard dose EC)₅₀Single agent EC₅₀×100

(3) Measured virulence index of mixed preparation is EC of standard single preparation₅₀EC of value/blend₅₀Value x 100

(4) The theoretical virulence index of the mixed preparation is the virulence index of the A preparation, the content of the A preparation in the mixed preparation, the virulence index of the B preparation and the content of the B preparation in the mixed preparation

(5) Co-toxicity coefficient (CTC) of mixed preparation is measured toxicity index/mixed preparation theory toxicity index x 100

Evaluating the value of the mixture according to the value of CTC, wherein the CTC is larger than 120 and indicates that the mixture has synergy, the CTC is smaller than 80 and indicates that the mixture has antagonism, and the CTC is between 80 and 120 and indicates that the mixture has additive effect, generally, the CTC of the mixture is more than 120 which is the basic requirement of pesticide compound development.

A is tebuconazole, B is triphenyl tin acetate; tebuconazole is taken as a standard medicament.

The indoor bioassay results are shown in table 1:

TABLE 1 indoor bioassay results of different ratios of triphenyltin acetate to tebuconazole for wheat sharp eyespot

	EC50(mg/mL)	Measured virulence index	Theoretical virulence index	Co-toxicity coefficient
					Tebuconazole (A)	7.8953	100	-	-
Triphenyltin acetate (B)	25.6328	30.8016	-	-
					A:B＝1:10	16.2135	48.6958	37.0923	131.2827
A:B＝2:1	5.3721	146.9686	76.9339	191.0324
					A:B＝1:2	11.3689	69.4465	53.8674	128.9212
A:B＝1:1	8.4672	93.2457	65.4008	142.5758
					A:B＝1:5	13.7643	57.3607	42.3347	135.4933

3. Field efficacy test of bactericidal composition containing triphenyltin acetate and tebuconazole

Field test example 1: drug effect test for preventing and controlling wheat sharp eyespot in field test

According to the wheat sharp eyespot pharmacodynamic test criterion, a proper test field is selected, the test is carried out in a wheat field in New and rural cities in Henan, the field soil fertility is consistent, the wheat growth vigor is good, the wheat is planted in the test field all the year round, and the occurrence condition of the wheat in a laboratory is an initial stage.

In a field efficacy test, 7 treatments of 25% of bactericidal composition wettable powder of triphenyltin acetate and tebuconazole (example 4), 45% of bactericidal composition wettable powder of triphenyltin acetate and tebuconazole (example 8), 80% of bactericidal composition wettable powder of triphenyltin acetate and tebuconazole (example 13), 35% of bactericidal composition dispersed oil suspending agent of triphenyltin acetate and tebuconazole (example 18), 60% of bactericidal composition dispersed oil suspending agent of triphenyltin acetate and tebuconazole (example 23), 25% of triphenyltin acetate dispersed oil suspending agent (F) and a clear water blank control are set, and each treatment is diluted by 500 times. The test areas are randomly arranged, the treatment is repeated for three times each time, and the stem and leaf spraying treatment is carried out according to the effective component dosage of 480 g/hectare.

The investigation, calculation and statistical method comprises the following steps: spraying the composition once every 7 days for 2 times at the initial stage of wheat stripe blight. For the experiments, 15 parallel experimental areas were selected, each area being administered according to table 2. The application method comprises the following steps: the dosage of the preparation required in the embodiment of each test area is calculated according to the dosage of the active ingredients, the preparation is uniformly sprayed on the test area in a manual mode (the application water dosage per hectare is 450kg), and the control preparation is applied in the same mode. The medicine is applied in the morning with a sunny day, the highest temperature of 26 ℃ and the lowest temperature of 12 ℃. Meanwhile, 1 area is selected as a blank control area, and no medicine is sprayed.

20 plants are sampled and investigated at 5 points by a per-cell line comparison method, 4 leaves are investigated on each plant, namely the upper leaf, the middle leaf and the lower leaf, the total leaf number and the leaf number of each stage of disease are recorded, and disease indexes are calculated respectively.

The grading method comprises the following steps:

level 0: no disease spots;

level 1: the area of the lesion spots accounts for less than 5% of the area of the whole leaf;

and 3, level: the area of the lesion spots accounts for 6 to 25 percent of the area of the whole leaf;

and 5, stage: the area of the lesion spots accounts for 26-50% of the area of the whole leaf;

and 7, stage: the area of the lesion spots accounts for 51-75% of the area of the whole leaf;

and 9, stage: the area of the lesion spots accounts for more than 75 percent of the area of the whole leaf;

investigation time and number of times:

the disease condition base is investigated before the application of the medicine, and the prevention and treatment effect is investigated 7 days after the last application of the medicine.

The drug effect calculation method comprises the following steps:

wherein:

CK₀-disease index before application in the clear water control zone;

CK₁-disease index after application in the clear water control zone;

PT₀-pre-dose disease index in the agent treatment area;

PT₁-disease index after drug treatment area application.

The test results are shown in table 2:

TABLE 2 fungicidal composition containing triphenyltin acetate and tebuconazole for preventing and controlling wheat sharp eyespot in pharmacodynamic test

As can be seen from the table 2, the bactericidal composition containing the triphenyltin acetate and the tebuconazole can effectively prevent and treat the wheat sharp eyespot, has better prevention and treatment effects than control agents, and has no adverse effect on target crops in the application range of the test. The control effect of the embodiment using the preferred auxiliary agent is better than that of other embodiments, and the control effect on the wheat sharp eyespot is more than 80%.

Field test example 2: pesticide effect test for preventing and controlling wheat powdery mildew in field test

The test results are shown in table 3:

TABLE 3 Bactericidal composition containing triphenyltin acetate and tebuconazole for preventing and treating wheat powdery mildew by pharmacodynamic test

As can be seen from Table 3, the bactericidal composition containing the triphenyltin acetate and the tebuconazole can effectively prevent and treat the wheat powdery mildew, has better prevention and treatment effects than those of a control medicament, and has no adverse effect on target crops in the application range of the test. The control effect of the embodiment using the preferable auxiliary agent is better than that of other embodiments, and the control effect on the wheat powdery mildew is more than 80%.

Field test example 3: drug effect test for preventing and controlling wheat sharp eyespot in field test

According to the wheat sharp eyespot pharmacodynamic test criterion, a proper test field is selected, the test is carried out in a wheat field in New and rural cities in Henan, the field soil fertility is consistent, the wheat growth vigor is good, the wheat is planted in the test field all the year round, and the occurrence condition of the wheat in a laboratory is an initial stage.

The field efficacy test is provided with 3 treatments, which are respectively as follows: treatment 1: spraying clear water, and blank control; and (3) treatment 2: the bactericidal compositions containing triphenyltin acetate and tebuconazole prepared in examples 6 and 21; and (3) treatment: the fungicides prepared in comparative examples 1 and 2; the test areas with the dilution of 800 times are randomly arranged, the treatment is repeated for three times each time, and the stem and leaf spraying treatment is carried out according to the effective component dosage of 480 g/hectare. The test results are shown in table 4:

TABLE 4

	Pre-drug disease index/%)	7 days after the first dose control%	7 days after the second application efficacy/%)
				Example 6	60.35	78.36	85.67
Example 21	52.34	69.34	83.25
				Comparative example 1	63.67	69.23	71.34
Comparative example 2	55.42	60.64	62.25

The investigation, calculation and statistical method comprises the following steps: spraying the composition once every 7 days for 2 times at the initial stage of wheat stripe blight.

4. Final residual quantity of bactericidal composition containing triphenyltin acetate and tebuconazole on crops and soil

Selecting wheat fields in Xinxiang city of Henan and Shaanxi Suiyang wheat fields, preparing standard solution with the concentration of 0.05, 0.1 and 1mg/kg from 80% of bactericidal composition wettable powder containing the triphenyltin acetate and the tebuconazole, respectively applying the standard solution to the wheat fields in the Xinxiang city of Henan and the Shaanxi Suiyang wheat fields, testing the residual quantity of the bactericidal composition containing the triphenyltin acetate and the tebuconazole in wheat and soil after applying the standard solution for a period of time, and testing results are shown in Table 5.

TABLE 5 Final residual amount of fungicidal composition containing triphenyltin acetate and tebuconazole on wheat and soil

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes may be made to the embodiment of the present invention by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.