阅读说明：本技术 一种具有防治抑郁功效的花茶及其制备方法 (Scented tea with depression preventing and treating effects and preparation method thereof ) 是由 汪豪 和寿祥 熊非 胡晓龙 王蓉 于 2020-12-08 设计创作，主要内容包括：本发明公开了一种具有防治抑郁功效的花茶及其制备方法。本发明采用药食同源的可用于食品及保健食品的植物作为有效组方,由下述重量份的原料制成：藏红花花瓣15-25份、玫瑰花5-15份、橙皮1-10份。本法制备而成的花茶具有疏肝理气之功效,对于抑郁症具有显著的预防和辅助治疗作用。(The invention discloses scented tea with a depression preventing and treating effect and a preparation method thereof. The invention adopts medicinal and edible plants which can be used for food and health food as effective components, and is prepared from the following raw materials in parts by weight: 15-25 parts of saffron petals, 5-15 parts of rose and 1-10 parts of orange peels. The scented tea prepared by the method has the efficacy of soothing liver and regulating qi, and has remarkable prevention and adjuvant treatment effects on depression.)

1. A scented tea with a depression preventing and treating effect is characterized by comprising the following components in parts by weight: 15-25 parts of saffron petals, 5-15 parts of rose and 1-10 parts of orange peels.

2. The scented tea with the effect of preventing and treating depression according to claim 1, is characterized by comprising the following components in parts by weight: 15-20 parts of saffron petals, 10-15 parts of rose and 5-10 parts of orange peels.

3. The scented tea with the effect of preventing and treating depression according to claim 2 is characterized by comprising the following components in parts by weight: 20 parts of saffron petals, 10 parts of rose and 5 parts of orange peels.

4. The preparation method of the scented tea of claim 1, which has the efficacy of preventing and treating depression, is characterized in that: removing impurities from the raw materials, cleaning, drying, slicing pericarpium Citri Junoris, and mixing with stigma croci Sativi petal and flos Rosae Rugosae at a certain ratio.

5. The application of the scented tea of claim 1, which has the efficacy of preventing and treating depression, in preparing medicines or health-care foods for preventing and treating depression.

Technical Field

The invention belongs to the field of medicines, and particularly relates to a scented tea with a depression preventing and treating effect and a preparation method thereof.

Background

Depressive disorder (MD), also known as depression, is a common mood disorder disease in psychiatric department, mainly manifested as significant and long-term low mood as a main clinical manifestation, seriously affecting the social function, occupational development and establishment of normal interpersonal relationship of patients, and bringing profound distress to patients and their families. In recent years, the prevalence of depression has been increasing, and it has been reported in the literature that the incidence of depression in developed countries exceeds even diseases such as tumors and cardiovascular and cerebrovascular diseases. At present, the chemical drug therapy can relieve depression symptoms to a certain extent, but the problems of high recurrence rate, large side effect and the like exist. Due to the limitations of chemical treatment of depression and many side effects, modern society begins to attach importance to the preventive treatment and health conditioning effects of traditional Chinese medicines. The subject is to research and develop anti-depression health products based on the traditional Chinese medicine inheritance and development concept, and has important research value and market prospect.

The Crocus sativus is a bulbar herbaceous plant of Crocus of Iridaceae, also called Crocus sativus and Crocus sativus, and the medicinal part of the Crocus sativus is dry stigma. The price of saffron is high, the petals of the non-medicinal part saffron are the dried petals of crocus sativus of the Iridaceae family, the yield is high, and no effective development and utilization way exists at present. The saffron petals are sweet in flavor, flat and nontoxic, and have the effects of promoting blood circulation to remove blood stasis, relieving depression and calming the nerves and the like. According to the records of Yin Teng Zheng Yao, saffron has the effect of treating heart-mind depression and qi stagnation. Long-term eating is happy. Modern researches show that saffron petals contain similar chemical components and biological activities of crocus stigma.

Rose flower, which is the dried bud of Rosa rugosa thumb. Sweet and slightly bitter taste, warm nature, liver and spleen meridian entered, has effects of promoting blood circulation and activating qi-flowing, dispersing stagnated liver qi and relieving qi stagnation, and aromatic resuscitation, and is mainly used for treating liver and stomach qi pain, new and chronic arthralgia due to wind-evil, hematemesis and hemoptysis, menoxenia, leucorrhea with reddish discharge, dysentery, acute mastitis, toxic swelling, depression, etc. In the book of Renzhen of materia medica, rose flower can soothe liver, gallbladder, qi stagnation, invigorate spleen and reduce fire. For cold pain in the abdomen, cold accumulation in the stomach and stomach, combined with blood breaking. Rose flower recorded in "diet book of living with rest" has the effects of regulating middle energizer, promoting blood circulation, dispersing stagnated qi, removing filth, and regulating liver.

Orange peel, also known as yellow peel, is the peel of Osbeck, Citrus sinensis (L.) of the rutaceae family. Warm in nature, pungent and slightly bitter in taste. Sour orange peel contains a large amount of vitamin A and can be used as a weak stomachic agent and an aromatic flavoring agent. Enter spleen and lung meridians and have the effects of promoting qi circulation and invigorating spleen. In the records of Sichuan Chinese materia medica, orange peel can promote qi circulation, resolve phlegm, invigorate spleen, warm stomach and treat inappetence. The recorded records in the book "read doctor with pen" the diameter from which the spleen ascends and descends. According to survey and statistics, the qi-regulating medicine is the medicine which is used most frequently in treating depression, and the orange peel becomes one of the traditional Chinese medicines used for treating depression in high frequency in the traditional Chinese medicine due to the mild qi-regulating effect of the orange peel.

Depression belongs to the category of emotional diseases in traditional Chinese medicine, the pathogenesis of the depression is closely related to liver loss, catharsis and qi and blood stagnation, and the treatment principle of the traditional Chinese medicine mainly emphasizes soothing the liver and regulating qi to restore the catharsis function to normal. The saffron petals and the roses have the effect of relieving depression; the rose also has the effect of soothing the liver and can be used for conditioning liver failure and diarrhea; the saffron petals and the roses can promote blood circulation, and the orange peels can regulate qi and regulate qi stagnation. These effects all have better prevention and treatment effects on depression.

The invention relates to a novel scented tea combination consisting of saffron petals, roses and orange peels which are medicinal and edible materials, relates to the pharmacological activity and the novel application of the combination, and is not reported at home and abroad.

Disclosure of Invention

The invention aims to solve the technical problem of providing the scented tea with the efficacy of preventing and treating depression and the preparation method thereof by adopting the plants which are homologous in medicine and food and can be used for health-care food as effective components.

The purpose of the invention is realized by the following modes:

a scented tea with an efficacy of preventing and treating depression comprises the following components in parts by weight: 15-25 parts of saffron petals, 5-15 parts of rose and 1-10 parts of orange peels.

Preferably, the scented tea is prepared from the following raw materials in parts by weight: 15-20 parts of saffron petals, 10-15 parts of rose and 5-10 parts of orange peels.

More preferably, the scented tea is prepared from the following raw materials in parts by weight: 20 parts of saffron petals, 10 parts of rose and 5 parts of orange peels.

The scented tea with the effect of preventing and treating depression can be prepared by adopting the conventional method, and is preferably prepared by the following method: removing impurities from the raw materials, cleaning, drying, and mixing at a certain proportion.

Compared with the prior art, the method has the following advantages:

1) according to the invention, single flowers with different effects are singly matched together according to a certain proportion, the appearance of the original flower medicinal materials is kept, the composite scented tea is researched and developed, and the orange peels and other medicinal flavors are supplemented, so that the unique health-care effect is achieved.

2) Different single products are matched together according to a certain proportion, so that better effect and taste can be achieved, and the drinking value of the scented tea is enhanced. The single scented tea raw material has poor efficacy and poor taste.

3) The formula of the scented tea product focuses on the curative effect and the taste. Aiming at the formula of the scented tea, the invention is repeatedly modulated and continuously adjusted, the proportion, the efficacy and the taste of the scented tea are continuously improved, animal experiments are carried out, the curative effect is observed, the invention has the function of preventing and treating depression, and the effect has obvious universality.

The use amount of each component in the scented tea with the effect of preventing and treating depression is summarized by the inventor through a large number of practices.

Drawings

FIG. 1 is a graph showing the protective effect of scented tea with single component and different components on apoptosis of PC12 induced by corticosterone.

Wherein the content of the first and second substances,^***p<0.001 was compared with the blank group,^#p<0.05 or^##p<0.01 was compared to the model group.

FIG. 2 is a graph of experimental results of sugar water preference for different scented tea groups.

Wherein p <0.001 is compared to the blank group and p <0.05 or # p <0.01 is compared to the model group.

Fig. 3 shows the results of the experiment on the change of the body weight of mice by different scented tea groups after unpredictable chronic mild stress experiments.

FIG. 4 shows the experimental results of the effects of different scented tea groups on the level of inflammatory factors in the hippocampus.

P <0.001 was compared to the blank group and # p <0.05 or # # p <0.01 was compared to the model group.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to examples, but those skilled in the art will appreciate that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention.

Example 1

Taking 20 parts by weight of saffron petals and 10 parts by weight of rose, carrying out microwave drying, taking 5 parts by weight of orange peel, slicing, feeding 3000g in total according to the formula proportion, mixing, packaging by adopting heat-sealing type tea filter paper, 3 g/bag, packaging in an outer packaging bag, and packaging by 10 bags and 1 bag to obtain a finished product.

Example 2

Taking 15 parts by weight of saffron petals and 15 parts by weight of rose, carrying out microwave drying, taking 10 parts by weight of orange peel, slicing, feeding 3000g in total according to the formula proportion, mixing, packaging by adopting heat-sealing type tea filter paper, 3 g/bag, packaging in an outer packaging bag, and packaging by 10 bags and 1 bag to obtain a finished product.

The effects of the present invention are further illustrated by the following tests:

research on comprehensive sensory quality of safflower petal depression relieving scented tea with different combination ratios

The preparation method of the safflower petal depression relieving scented tea comprises the steps of microwave drying the safflower petals and the rose, slicing the orange peel, feeding 3000g in total according to the formula proportion, mixing, packaging by adopting heat-sealing type tea filter paper, 3 g/bag, packaging in an outer packaging bag, and packaging by 10 bags and 1 bag to obtain the finished product.

(1) Experimental Material

Weighing saffron petals, roses and orange peels according to the table 1, adding 50mL of 90 ℃ pure water, fully soaking for 30min, filtering, collecting filtrate, and preparing scented tea samples with different ratios.

TABLE 1 different combination ratio table of saffron petal melancholy scented tea

Proportioning ratio	Saffron petal	Rose flower	Orange peel
				Ratio 1	20g	10g	5g
Ratio 2	15g	10g	5g
				Ratio 3	25g	10g	5g
Ratio 4	20g	5g	5g
				Ratio 5	20g	15g	5g
Ratio 6	20g	10g	1g
				Ratio 7	20g	10g	10g

(2) Experimental methods

Comprehensively establishing a sensory evaluation table (table 1) of the saffron petal depression relieving scented tea according to GB/T23776-2009 tea sensory evaluation method and the characteristics of the scented tea, randomly selecting 10 laymen as examiners, observing soup color, smelling fragrance in a cup, tasting taste, scoring according to table 2, and taking the average score of the scores as a statistical result.

TABLE 2 evaluation table for sensory quality of flos croci petal melancholy dispelling scented tea

(3) Results of the experiment

The 10 panelists scored different combinations of saffron petal melancholy teas according to the scoring criteria of table 2, and the average score was multiplied by a weighting factor, with the results shown in table 3. In the aspect of liquor color, the crocus petal depression relieving flower tea soup obtained by combining and proportioning is light yellow to light orange, and has higher brightness, so the obtained scores are higher. In terms of fragrance, the crocus petal depression relieving flower tea soup prepared according to the ratio 6 is weak in fragrance, the rest fragrance is rich and pure, and the orange peels with fragrance and qi circulation promoting effects contained in the ratio 6 are possibly less, so that the whole fragrance is not strong enough. In terms of taste, the mouth feel scores of the saffron petal depression relieving flower tea soup prepared according to the proportion 5 and the proportion 7 are slightly lower, probably because the proportion of the rose flower with sweet and slightly bitter taste in the proportion 5 is higher, the proportion of the orange peel with slightly astringent mouth feel in the proportion 7 is higher, and the sample tea soup prepared according to the rest proportions is mellow and fresh, so that higher scores are obtained. On the comprehensive score, the saffron petal depression relieving flower tea soup prepared according to the proportion of 1, 2 and 3 has good comprehensive taste, the score is over 90, and the comprehensive score of the proportion of 1 is the highest; the saffron petal depression relieving flower tea soup prepared in the ratio of 4 to 7 has a good comprehensive taste, and the score is 85 to 90; the crocus sativus petal depression relieving scented tea soup prepared in the ratio of 5 has a common comprehensive taste, and the score is between 80 and 85.

TABLE 3 comprehensive sensory quality scoring results of different combination ratios of saffron petal and melancholy-dispelling scented tea

In conclusion, the saffron petal depression relieving flower tea soup prepared by the plurality of proportions has different comprehensive tastes, and the comprehensive score is highest in proportion 1.

Experimental example 1 study on in vitro antidepressant activity of different combinations of saffron petal depression-relieving scented tea

(1) Experimental Material

The scented tea extract with single component and the scented tea with different components are prepared by laboratories. The process is as follows: weighing 20g of saffron petals, 10g of rose and 5g of orange peel, adding 50mL of 90 ℃ pure water, fully soaking for 30min, filtering, and collecting filtrate to obtain a scented tea group 1; weighing 20g of saffron petals, and preparing into a scented tea group 2 by the same method; weighing 10g of rose, and preparing by the same method to obtain a scented tea group 3; weighing 5g of orange peel, and preparing into a scented tea group 4 by the same method; weighing 20g of saffron petals and 10g of roses, and preparing into a scented tea group 5 by the same method; weighing 20g of saffron petals and 5g of orange peel, and preparing into a scented tea group 6 by the same method; 10g of rose and 5g of orange peel are weighed and prepared according to the same method to obtain the scented tea group 7. Fluoxetine hydrochloride tablets (10 mg/tablet, febuxostat, four drugs pharmaceuticals, ltd.).

(2) Experimental methods

PC12 cells were diluted to 2X 10 cells per ml suspension in DMEM medium containing 10% fetal bovine serum⁵Inoculating the cells into a 96-well culture plate, culturing in a 5% CO2 incubator at 37 ℃ with 100 mu L of each well, removing cell liquid after the cells grow to the bottom of the well, adding a culture solution containing 400 mu M corticosterone (Cort), incubating for 24h, removing the culture solution, adding serum-free DMEM containing 0.5 mg/mL-1 MTT into each well, culturing for 4h, removing DMEM,after the blue particles were completely dissolved, 100. mu.L of dimethyl sulfoxide (DMSO) was added to each well, and the absorbance (A) of the sample at a wavelength of 570nm was measured by a microplate reader to calculate the cell viability.

Cell survival (%) × 100% (experimental group a value/normal group a value).

The cell experiment is divided into (1) normal control group, which comprises sucking off original cell culture fluid and changing to complete culture medium; (2) in the corticosterone group, the original cell culture solution is aspirated and replaced by a complete culture medium containing 400 mu M corticosterone; (3) in fluoxetine group, the original cell culture solution is aspirated and replaced by a complete culture medium containing 400 μ M of corticosterone, and fluoxetine (50 μ M) is added at the same time; test drug groups: the stock cell culture was aspirated and replaced with 400. mu.M complete medium containing corticosterone, while different drugs (200. mu.g/mL) were added. After adding the drug, the cell survival rate is detected by MTT after the culture is continued for 24 hours.

(3) Results of the experiment

After the PC12 cells are damaged by 400 mu M corticosterone for 24h, the cell survival rate is obviously lower than that of a normal control group, and the difference is statistically significant compared with the control group (P < 0.001). As can be seen from fig. 1, the scented tea combination groups 1, 3, 4, 5, 6, and 7 all have certain activities, and the differences have statistical significance (P <0.05) compared with the corticosterone group, wherein the scented tea combination groups 1, 6, and 7 are more obvious, and the three-component scented tea combination group 1 is the most preferable. Therefore, the efficacy of the single-component scented tea for resisting corticosterone-induced PC12 apoptosis is inferior to that of two scented tea combinations, and the effect of the three scented tea combinations for improving corticosterone-induced apoptosis is the best. The experimental results show that the three-component combined scented tea has better in-vitro antidepressant activity, and the three-component combined scented tea has better synergistic effect.

Experimental example 2 influence of saffron petal depression relieving scented tea on chronic unpredictable stress-induced depression mice

(1) Experimental Material

The experimental animal SPF-grade BALB/c mouse is male, has the weight of 18-22 g, and is purchased from model animal center of Nanjing university. Scented tea with different components of medicines and reagents is prepared by laboratories, and the specific process is as follows: weighing 20g of saffron petals, 10g of rose and 5g of orange peel, adding 50mL of 90 ℃ pure water, fully soaking for 30min, filtering, and collecting filtrate to obtain a scented tea group 1; weighing 20g of saffron petals, preparing according to the same method, weighing 2g of scented tea, weighing 10g of rose, preparing according to the same method, weighing 3g of scented tea, weighing 5g of orange peel, preparing according to the same method, weighing 4 g of scented tea, weighing 20g of saffron petals and 10g of rose, preparing according to the same method, weighing 5g of saffron petals and 5g of orange peel, preparing according to the same method, weighing 6 g of scented tea, weighing 10g of rose and 5g of orange peel, preparing according to the same method, and preparing 7 g of scented tea. Fluoxetine hydrochloride tablets (10 mg/tablet, febuxostat, four drugs pharmaceuticals, ltd.). Mouse IL-1 β, IL-6 and TNF- α ELISA kits were purchased from Wuhan Huamei.

(2) Experimental methods

Grouping, dosing and modeling 120 Balbc mice were randomly divided into 10 groups (12/group) by body weight: a blank control group, a model control group, a positive drug (fluoxetine) control group, a scented tea 1 group, a scented tea 2 group, a scented tea 3 group, a scented tea 4 group, a scented tea 5 group, a scented tea 6 group and a scented tea 7 group. The scented tea is administrated by intragastric administration for 3 times every day from day 1, each time is 0.2 mL/flower, the blank group and the model group are administrated with purified water with the same volume, and the positive drug group is administrated with fluoxetine 10mg/kg 1 time every day. Chronic unpredictable stress stimulation started on day 31, following the following specific procedure: mice were subjected to different types of stress: restraint was 4 hours, cage tilt 24 hours, wet bedding 24 hours, swimming 5 minutes in cold water at 4 ℃, swimming 5 minutes in hot water at 45 ℃, shaking horizontally for 10 minutes, tail compression for 1 minute, light/dark cycle inversion for 24 hours, fasted and water deprived overnight. Mice received one of these pressures daily, these 9 pressures were randomly applied for 30 days, and the same pressure was not applied for two consecutive days, to minimize the predictability of each pressure. Three-day behavioural assessment from day 61 onwards, the procedure was as follows 1) sucrose preference test: firstly, carrying out sugar water adaptability exercise, and forbidding water for 24 h. The test was started after 24h, two bottles of 100mL water, one bottle of 1% sugar water and one bottle of pure water, were placed in each mouse cage, and the recording was started after 2h, and the consumption of sugar water and pure water was recorded, respectively. In the experimental process, the amount of each bottle of water needs to be carefully and accurately read, the horizontal placement position needs to be kept consistent, and the mice can be guaranteed to normally drink water. 2) Forced swimming test: the mice were placed individually in 40cm x 20cm glass jars (water 20cm, water temperature around 25 ℃), their activity was observed over 6 minutes using a small animal behavioral analysis system (Etho-Vision XT9), and the fixed time was recorded over the last 4 minutes over 6 minutes. Mice are judged to be standing still when they stop struggling and float on the water surface or only do some small movements to keep their heads on the water. After the experiment of each rat is finished, the water tank needs to be cleaned, water needs to be changed, and then the next rat is tested continuously to avoid interference. 3) And (3) tail suspension test: a small clamp is connected with the center of the top plate of the tail suspension box (25cm multiplied by 35 cm). The tail part of the mouse is stuck to the position 2cm away from the tail tip by using an adhesive tape, the adhesive tape is clamped by using a clamp, the mouse is suspended in a black tail suspension box, and the head part of the mouse is 5cm away from the box bottom. The animals were observed for activity within 6 minutes using the MedLab BioInformatics collection System (MedLab-U/4C501H, Napkinsonkyu technologies, Inc. China) and the time to immobility was recorded for the last 4 minutes. The mouse is fixed when the mouse stops struggling and is in a static vertical suspension state. Mouse hippocampal tissue was isolated starting at day 64 and assayed for levels of the relevant inflammatory factors IL-1 β, IL-6 and TNF- α.

(3) Results of the experiment

a) Influence of different scented tea groups on sucrose preference experiment

As shown in fig. 2, the sugar water preference index of the mice in the model control group was significantly decreased compared to the normal control group. Compared with a model control group, the fluoxetine hydrochloride group and the scented tea group have obviously improved sucrose preference index of mice. The scented tea group 1 has a particularly obvious effect. The results show that the scented tea group 1 consisting of 20g of saffron petals, 10g of roses and 5g of orange peels is obviously superior to a single component or the combination of two components.

b) Influence of different scented tea groups on forced swimming experiment

TABLE 4 forced swimming and tail suspension immobility time of mice

^**p<0.01 is compared to the blank set,^#p<0.05 was compared to the model set.

As shown in table 4, the activity resting time of the model group mice was significantly prolonged (P <0.01) compared to the normal control group. Compared with the model control group, the fluoxetine group and the scented tea group can obviously shorten the resting time. The immobility time of the scented tea 1 is obviously reduced. The results show that the scented tea group 1 consisting of 20g of saffron petals, 10g of roses and 5g of orange peels is obviously superior to a single component or the combination of two components.

c) Influence of different scented tea groups on tail suspension test experiment

As shown in table 4, the increased immobility time of the tail suspension test experiment in the model group mice was significantly prolonged compared to the normal control group. Compared with a model control group, the fluoxetine group and the scented tea group can obviously shorten the immobility time. Compared with other scented tea groups, scented tea 1 obviously reduces immobility time of mice. The results show that the scented tea group 1 consisting of 20g of saffron petals, 10g of roses and 5g of orange peels is obviously superior to a single component or the combination of two components.

d) Unpredictable weight change after chronic mild stress of different scented tea groups

As shown in FIG. 3, the body weight of the mice in the model group was significantly reduced, and the body weight of the mice in the fluoxetine (10mg/kg) and scented tea group was increased compared to the body weight of the mice in the model group. The mice in scented tea group 1 had insignificant weight loss. The results show that the scented tea group 1 consisting of 20g of saffron petals, 10g of roses and 5g of orange peels is obviously superior to a single component or the combination of two components.

e) Influence of different scented tea groups on inflammatory factor level in hippocampus

As shown in FIG. 4, the levels of IL-1 β, IL-6, TNF- α were significantly increased in the hippocampus of the model group (P <0.05) compared to the normal control group. Compared with the model group, the content of the hippocampus of the mouse in the scented tea group is obviously reduced (P is less than 0.05), wherein the scented tea group 1 is most obviously reduced. The results show that the scented tea group 1 consisting of 20g of saffron petals, 10g of roses and 5g of orange peels is obviously superior to a single component or the combination of two components.

The experiments fully confirm that the scented tea composed of the saffron petals, the roses and the orange peels has a synergistic effect and has a remarkable anti-depression effect.