阅读说明：本技术 来自植物的杀昆虫蛋白及其使用方法 (Insecticidal proteins from plants and methods of use thereof ) 是由 J·K·巴里 董华 R·M·格尔伯 B·彼得森-伯奇 E·谢珀斯 T·C·沃尔夫 谢卫平 于 2019-03-12 设计创作，主要内容包括：本发明提供了用于防治有害生物的组合物和方法。所述方法涉及用编码杀昆虫蛋白的核酸序列来转化生物体。特别地,所述核酸序列可用于制备具有杀昆虫活性的植物和微生物。因此,提供了经转化的细菌、植物、植物细胞、植物组织以及种子。组合物是细菌物种的杀昆虫核酸和蛋白。所述序列可用于构建随后转化到包括植物在内的目的生物体中的表达载体,作为用于分离其他同源(或部分同源的)基因的探针。所述杀有害生物蛋白可用于防治鳞翅目、鞘翅目、双翅目、真菌、半翅目和/或线虫有害生物群体,抑制其生长或将其杀灭,并且可用于生产具有杀昆虫活性的组合物。(The present invention provides compositions and methods for controlling pests. The methods involve transforming organisms with nucleic acid sequences encoding insecticidal proteins. In particular, the nucleic acid sequences are useful for the preparation of plants and microorganisms having insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues, and seeds are provided. The compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences can be used to construct expression vectors for subsequent transformation into organisms of interest, including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins are useful for controlling, inhibiting the growth of, or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and/or nematode pest populations, and for producing compositions having insecticidal activity.)

1. A recombinant insecticidal polypeptide, or a fragment thereof having insecticidal activity, comprising a sequence that hybridizes to seq id NO: 2 with at least 80% sequence identity.

2. The recombinant insecticidal polypeptide of claim 1, wherein the amino acid sequence is identical to SEQ ID NO: 2 have at least 95% sequence identity.

3. The recombinant insecticidal polypeptide of claim 1, wherein the fragment comprises a sequence corresponding to SEQ ID NO: 2, amino acids 61-472.

4. The recombinant insecticidal polypeptide of claim 1, 2, or 3, wherein the insecticidal polypeptide is linked to a heterologous signal sequence or transport sequence.

5. The recombinant insecticidal polypeptide of claim 1, wherein the polypeptide has insecticidal activity against a lepidopteran agricultural pest.

6. A chimeric insecticidal protein comprising at least two different portions of the recombinant insecticidal polypeptide of claim 1, 2, or 3.

7. A fusion protein comprising the recombinant insecticidal polypeptide of claim 1, 2, or 3.

8. An agricultural composition comprising at least one recombinant insecticidal polypeptide of claim 1, 2, or 3.

9. A recombinant polynucleotide encoding the insecticidal polypeptide of claim 1, 2 or 3.

10. The recombinant polynucleotide of claim 9, wherein the polynucleotide has codons optimized for expression in an agriculturally important crop.

11. The recombinant polynucleotide of claim 9, wherein the polynucleotide is a cDNA.

12. A recombinant polynucleotide encoding the chimeric insecticidal protein of claim 6.

13. A DNA construct comprising the recombinant polynucleotide of claim 9, 10 or 11.

14. A transgenic plant comprising the polynucleotide of claim 9, 10 or 11.

15. A transgenic plant comprising the DNA construct of claim 13.

16. A DNA construct comprising the recombinant polynucleotide of claim 12.

17. A transgenic plant comprising the polynucleotide of claim 12.

18. A transgenic plant comprising the DNA construct of claim 16.

19. A method of inhibiting the growth of or killing an insect pest or pest population, comprising contacting the insect pest with the insecticidal polypeptide of claim 1, 2, or 3.

20. A method of inhibiting the growth of or killing an insect pest or pest population, the method comprising expressing in a plant the polynucleotide of claim 9, 10, or 11.

21. A method of controlling damage to a plant by an insect pest, the method comprising providing for ingestion the insecticidal polypeptide of claim 1, 2, or 3 to an insect pest or pest population, wherein the insecticidal polypeptide is produced by a transgenic plant and is present in at least one of the plants.

22. A method for controlling pest infestation, the method comprising providing a transgenic plant of claim 14 or 15, or a part thereof, in the diet of said pest.

23. A method for increasing the yield of a crop, the method comprising growing the transgenic plant of claim 14 or 15, wherein the yield of the crop is increased in the presence of insect pests relative to a crop not comprising the transgenic plant.

24. The method of any one of claims 19-23, wherein said insect pest or pest population is resistant to at least one Cry insecticidal protein.

25. The method of any one of claims 19-24, wherein the transgenic plant is selected from the group consisting of maize, soybean, wheat, rice, sorghum, sunflower, canola, barley, sugarcane, potato, tomato, cotton, rapeseed, peanut, and alfalfa.

26. The method of any one of claims 19-25, wherein the insect pest or insect pest population is an agriculturally important species in the order lepidoptera.

27. The method of claim 26, wherein the insect pest or insect pest population is selected from the group consisting of corn earworm, european corn borer, fall armyworm, soybean looper, velvet bean looper, and diamond-back moth.