阅读说明：本技术 一种抗龋齿保健糖果及其制备方法 (Anti-caries health-care candy and preparation method thereof ) 是由 崔春兰 郑虎哲 郑朝尹 罗馨 李正艳 于 2021-08-30 设计创作，主要内容包括：本发明公开了一种抗龋齿保健糖果及其制备方法,属于保健技术领域,该抗龋齿保健糖果由以下重量份的原料组成：苹果多酚-壳寡糖微胶囊5～15份、金银花30～60份、菊花20～40份、枸杞子20～40份、黄芪20～40份、石斛20～40份、薄荷20～40份、麦冬20～40份、白砂糖20～60份、木糖醇10～30份、色素0.1～0.5份、香精0.1～0.5份、浓缩果汁10～30份、吉利丁片10～20份；通过对原料药材进行预处理、熬制原料药材、原料液溶糖转变为糖膏,糖膏中添加附加原料,注模成型,冷却后制成该抗龋齿保健糖果；本发明制备的抗龋齿保健糖果抗菌效果较好,携带食用方便,适合工厂化生产,并能较好地适应市场需求。(The invention discloses an anti-caries health-care candy and a preparation method thereof, belonging to the technical field of health care, wherein the anti-caries health-care candy is prepared from the following raw materials in parts by weight: 5-15 parts of apple polyphenol-chitosan oligosaccharide microcapsules, 30-60 parts of honeysuckle, 20-40 parts of chrysanthemum, 20-40 parts of wolfberry fruit, 20-40 parts of astragalus membranaceus, 20-40 parts of dendrobe, 20-40 parts of mint, 20-40 parts of radix ophiopogonis, 20-60 parts of white granulated sugar, 10-30 parts of xylitol, 0.1-0.5 part of pigment, 0.1-0.5 part of essence, 10-30 parts of concentrated fruit juice and 10-20 parts of gilliding tablets; the anti-caries health-care candy is prepared by pretreating raw medicinal materials, decocting the raw medicinal materials, dissolving sugar in raw material liquid, converting the raw medicinal materials into massecuite, adding additional raw materials into the massecuite, performing injection molding, and cooling; the anti-caries health-care candy prepared by the invention has good antibacterial effect, is convenient to carry and eat, is suitable for industrial production, and can better adapt to market demands.)

1. The anti-caries health-care candy is characterized by comprising the following raw materials in parts by weight: 5-15 parts of apple polyphenol-chitosan oligosaccharide microcapsules, 30-60 parts of honeysuckle, 20-40 parts of chrysanthemum, 20-40 parts of wolfberry fruit, 20-40 parts of astragalus membranaceus, 20-40 parts of dendrobe, 20-40 parts of mint, 20-40 parts of radix ophiopogonis, 20-60 parts of white granulated sugar, 10-30 parts of xylitol, 0.1-0.5 part of pigment, 0.1-0.5 part of essence, 10-30 parts of concentrated fruit juice and 10-20 parts of gilliding tablets.

2. A method for preparing the anti-caries health-care candy as claimed in claim 1, which comprises the following steps:

s1 preparation of apple polyphenol-chitosan oligosaccharide microcapsules

Sufficiently dissolving apple polyphenol and chitosan oligosaccharide, and spray drying to obtain apple polyphenol-chitosan oligosaccharide microcapsules;

s2, pretreatment of raw materials

Mixing apple polyphenol-chitosan oligosaccharide microcapsules, honeysuckle, chrysanthemum, wolfberry fruit, astragalus, dendrobium, mint and radix ophiopogonis according to the formula amount, and then soaking in water to obtain a pretreated raw material;

s3, decocting the raw materials

Adding water into the raw material pretreated by the step S2, decocting, and filtering to obtain a raw material solution;

s4 preparation of anti-caries health candy

Adding the white granulated sugar with the formula amount into the raw material liquid obtained in the step S3, dissolving the sugar, converting the dissolved sugar into a massecuite, adding the xylitol, the pigment, the essence, the concentrated fruit juice and the gilidine sheet into the massecuite according to the formula amount, uniformly dispersing to obtain a mixed sugar body, carrying out injection molding on the mixed sugar body, and cooling to obtain the anti-caries health-care candy.

3. The preparation method of the anti-caries health-care candy as claimed in claim 2, wherein in S1, the mass ratio of the apple polyphenol to the chitosan oligosaccharide is 1: 2-3.

4. The method for preparing an anti-caries health candy as claimed in claim 2, wherein in S1, the spray drying parameters are: the feeding amount is 110-130 m³The air inlet temperature is 140-160 ℃, and the feeding rate is 240-260 mL/h.

5. The preparation method of the anti-caries health-care candy as claimed in claim 2, wherein in S2, water which is 7-8 times of the weight of the raw materials is added into the raw materials.

6. The method for preparing a health candy for preventing dental caries according to claim 2, wherein the soaking condition in S2 is 50-60 ℃ for 0.5-1 h.

7. The preparation method of the anti-caries health-care candy as claimed in claim 2, wherein in S3, the decocting time is 1-2 h.

8. The method for preparing a health confection for preventing dental caries according to claim 2, wherein the confection has a viscosity of 2000 to 2500cP and a temperature of 50 to 80 ℃ in S4.

Technical Field

The invention relates to the technical field of health care, in particular to an anti-dental caries health care candy and a preparation method thereof.

Background

Dental caries is the most common representative disease of teeth, and particularly, the incidence rate of dental caries tends to increase year by year along with the increase of the using amount of a sweetening agent in the process of food development. At present, more than half of adults worldwide suffer from dental diseases, of which about the majority of the causes of major diseases leading to deterioration of oral health are caused by dental caries. Mutans streptococci are the main causative agent of early caries formation, and the glucosyltransferase secreted by mutans streptococci is based on sucrose, synthesizes adherent, water-insoluble glucose, and forms a bacterial biofilm in the oral cavity. With the gradual understanding and understanding of people on the health of teeth, the anti-dental caries health care product is gradually favored and concerned by consumers, and has a broad prospect.

The content of polyphenols (apple polyphenol) in unripe apple is more than 10 times that in ripe apple, and the polyphenols have multiple functions of anti-aging, anti-inflammation, anti-pathogenic bacteria, anti-tumor, anti-mutation and the like. Chitosan oligosaccharide is deacetylated chitin oligosaccharide obtained by decomposing chitosan, has the performances of no toxicity, oxidation resistance, bacteriostasis, easy biodegradation, biocompatibility and the like, and is widely concerned in the research and development fields of foods and new medicinal preparations. Chitosan oligosaccharide has bacteriostatic action, but has weak bacteriostatic ability compared with traditional chemical preservatives and natural plant extracts. Apple polyphenol and chitosan oligosaccharide are not widely applied to the research and development of anti-caries health products. Therefore, there is a need to develop a health candy which has the health efficacy of anti-caries and is convenient to carry and eat.

Disclosure of Invention

The invention provides an anti-caries health-care candy and a preparation method thereof, which effectively inhibit the activity of glucosyltransferase, reduce the adhesion capability of thalli and the forming capability of a bacterial biofilm on a tooth surface and provide the anti-caries health-care candy which is convenient to carry and eat.

The invention provides an anti-caries health-care candy which is characterized by comprising the following raw materials in parts by weight: 5-15 parts of apple polyphenol-chitosan oligosaccharide microcapsules, 30-60 parts of honeysuckle, 20-40 parts of chrysanthemum, 20-40 parts of wolfberry fruit, 20-40 parts of astragalus membranaceus, 20-40 parts of dendrobe, 20-40 parts of mint, 20-40 parts of radix ophiopogonis, 20-60 parts of white granulated sugar, 10-30 parts of xylitol, 0.1-0.5 part of pigment, 0.1-0.5 part of essence, 10-30 parts of concentrated fruit juice and 10-20 parts of gilliding tablets.

The invention also provides a preparation method of the anti-caries health-care candy, which is characterized by comprising the following steps of:

s1 preparation of apple polyphenol-chitosan oligosaccharide microcapsules

Sufficiently dissolving apple polyphenol and chitosan oligosaccharide, and spray drying to obtain apple polyphenol-chitosan oligosaccharide microcapsules;

s2, pretreatment of raw materials

Mixing apple polyphenol-chitosan oligosaccharide microcapsules, honeysuckle, chrysanthemum, wolfberry fruit, astragalus, dendrobium, mint and radix ophiopogonis according to the formula amount, and then soaking in water to obtain a pretreated raw material;

s3, decocting the raw materials

Adding water into the raw material pretreated by the step S2, decocting, and filtering to obtain a raw material solution;

s4 preparation of anti-caries health candy

Adding the white granulated sugar with the formula amount into the raw material liquid obtained in the step S3, dissolving the sugar, converting the dissolved sugar into a massecuite, adding the xylitol, the pigment, the essence, the concentrated fruit juice and the gilidine sheet into the massecuite according to the formula amount, uniformly dispersing to obtain a mixed sugar body, carrying out injection molding on the mixed sugar body, and cooling to obtain the anti-caries health-care candy.

Preferably, in S1, the mass ratio of the apple polyphenol to the chitosan oligosaccharide is 1: 2-3.

Preferably, in S1, the spray drying parameters are: the feeding amount is 110-130 m³The air inlet temperature is 140-160 ℃, and the feeding rate is 240-260 mL/h.

Preferably, in S2, water is added to the raw material in an amount of 7 to 8 times by weight of the raw material.

Preferably, in S2, the soaking time is 0.5-1 h at 50-60 ℃.

Preferably, in S3, the boiling time is 1-2 h.

Preferably, in S4, the massecuite has a viscosity of 2000-2500 cP and a temperature of 50-80 ℃.

The utility analysis of each raw material in the formula is as follows:

apple polyphenol: has the health-care functions of preventing hypertension, resisting tumors, aging, mutation, radiation and allergy, preventing decayed teeth, enhancing muscle strength and the like.

Chitosan oligosaccharide: has effects in resisting dental caries, reducing blood lipid, lowering blood sugar, lowering blood pressure, improving digestive function, loosening bowel, and relieving constipation.

Honeysuckle flower: sweet in taste; cold in nature; entering lung and stomach meridians; has effects of clearing away heat and toxic materials, relieving inflammation, and relieving swelling.

Chrysanthemum: sweet and slightly bitter in taste, slightly cold in nature and entering lung and liver meridians; has the functions of dispersing wind, clearing away heat, calming liver, improving eyesight, clearing away heat and toxic material, etc.

Wolfberry fruit: sweet in flavor, neutral in nature, entering liver and kidney meridians; has effects of nourishing liver and kidney, replenishing vital essence, and improving eyesight.

Astragalus root: sweet in taste, slightly warm in nature, and entering spleen and lung meridians; has the effects of invigorating qi, consolidating exterior, expelling toxin, expelling pus, promoting urination, promoting granulation, etc.

Mint: pungent taste and cool nature, entering lung and liver meridians; has the effects of dispelling wind and heat, clearing head and eyes, relieving sore throat, promoting eruption, soothing liver, promoting qi circulation and the like.

Dendrobe: sweet in taste, slightly cold in nature, entering stomach and kidney meridians; has the effects of benefiting stomach, promoting fluid production, nourishing yin, clearing heat and the like.

Radix ophiopogonis: sweet and bitter in flavor, slightly cold in nature, entering heart, lung and stomach meridians; has the effects of nourishing yin, promoting the production of body fluid, moistening lung, relieving cough and the like.

Xylitol: xylitol can prevent dental caries. The metabolism is not regulated by insulin, is completely metabolized in human body, has low calorie, and can be used as heat energy source for diabetes patients.

The raw material medicines are properly matched, and the nature and flavor of the medicines are supplemented by the channels; clear heat and relieve swelling, nourish yin and tonify kidney. Honeysuckle flower and chrysanthemum flower, flos Lonicerae, flos Chrysanthemi, flos Lonicerae, flos Chrysanthemi, and flos Chrysanthemi, wherein flos Lonicerae, flos Chrysanthemi, and flos Chrysanthemi. The mint has the effects of dispelling wind and dissipating heat and has the effect of clearing heat together with monarch drugs; the kidney governs bones to generate marrow, the teeth are the rest of bones, the medlar nourishes liver and kidney, strengthens teeth and prevents alopecia, and the two medicines are used as ministerial medicines together. Radix astragali tonifies qi and strengthens exterior, and radix ophiopogonis and dendrobium stem nourish yin and clear heat and tonify qi and yin are used as adjuvant drugs together. The medicines are compatible to play the effects of clearing heat and reducing swelling, and nourishing yin and tonifying kidney. The formula can play a role in preventing dental caries by matching with apple polyphenol.

Compared with the prior art, the invention has the beneficial effects that:

(1) the apple polyphenol-chitosan oligosaccharide microcapsules are synthesized from the apple polyphenol and the chitosan oligosaccharide in the anti-caries health-care candy provided by the invention in a mode of Van der Waals force and intermolecular hydrogen bonds, and the substance has functional groups of the apple polyphenol and the chitosan oligosaccharide and synergistically enhances the bacteriostatic activity of the anti-caries health-care candy.

(2) The minimum inhibitory concentration of the anti-dental caries health-care candy prepared by the invention to streptococcus mutans is 7.1mg/ml, the inhibitory concentration to glucosyltransferase activity is 2.2mg/ml, the inhibitory concentration to thallus adhesion is 1.9mg/ml, and the inhibitory concentration to dental surface bacterial biofilm formation is 1.1 mg/ml.

(3) The anti-dental caries health care candy provided by the invention is simple in preparation method, convenient to carry and eat, good in anti-dental caries performance, suitable for industrial production and capable of well meeting market requirements.

Drawings

FIG. 1 is a scanning electron micrograph: (a) scanning electron microscope images of chitosan oligosaccharide, apple polyphenol and apple polyphenol-chitosan oligosaccharide microcapsules are respectively shown in (a) to (c);

FIG. 2 is a Fourier transform infrared absorption spectrum of chitosan oligosaccharide, apple polyphenol and apple polyphenol-chitosan oligosaccharide microcapsules;

fig. 3 is a minimal inhibitory concentration chart of chitosan oligosaccharide, apple polyphenol and apple polyphenol-chitosan oligosaccharide microcapsules: (a) and (c) are the minimum inhibitory concentration graphs of the chitosan oligosaccharide, the apple polyphenol and the apple polyphenol-chitosan oligosaccharide microcapsules respectively.

Detailed Description

In order to make the technical solutions of the present invention better understood and enable those skilled in the art to practice the present invention, the following embodiments are further described, but the present invention is not limited to the following embodiments.

The following test methods and detection methods, unless otherwise specified, are conventional methods; the reagents and starting materials are all commercially available, unless otherwise specified.

Example 1

An anti-caries health-care candy is composed of the following raw materials in parts by weight: 5 parts of apple polyphenol-chitosan oligosaccharide microcapsules, 30 parts of honeysuckle, 20 parts of chrysanthemum, 20 parts of wolfberry fruits, 20 parts of astragalus membranaceus, 20 parts of dendrobium, 20 parts of mint, 20 parts of radix ophiopogonis, 20 parts of white granulated sugar, 10 parts of xylitol, 0.1 part of pigment, 0.1 part of essence, 10 parts of concentrated fruit juice and 10 parts of gili tablet.

A preparation method of an anti-caries health-care candy comprises the following steps:

s1 preparation of apple polyphenol-chitosan oligosaccharide microcapsules

Sufficiently dissolving apple polyphenol and chitosan oligosaccharide according to the mass ratio of 1:2, and performing spray drying to obtain the apple polyphenol-chitosan oligosaccharide microcapsule, wherein the spray drying parameters are as follows: the feed rate was 110m³The air inlet temperature is 140 ℃, and the feeding rate is 240 mL/h;

s2, pretreatment of raw materials

Adding apple polyphenol-chitosan oligosaccharide microcapsules, honeysuckle, chrysanthemum, wolfberry fruit, astragalus, dendrobium, mint and radix ophiopogonis into water with the temperature of 50 ℃ according to the weight ratio of 1:7, and soaking for 0.5h to obtain a pretreated raw material;

s3, decocting the raw materials

Adding water into the raw material pretreated by the step S2, decocting, and filtering to obtain a raw material solution;

s4 preparation of anti-caries health candy

Adding the white granulated sugar with the formula amount into the raw material liquid obtained in the step S3, dissolving the sugar, converting the dissolved sugar into massecuite, adding the xylitol, the pigment, the essence, the concentrated fruit juice and the gilding into the massecuite according to the formula amount when the viscosity of the massecuite is 2000cP and the temperature is 50 ℃, dispersing uniformly to obtain a mixed sugar body, carrying out injection molding on the mixed sugar body, and cooling to obtain the anti-dental caries health-care candy.

Example 2

An anti-caries health-care candy is composed of the following raw materials in parts by weight: 15 parts of apple polyphenol-chitosan oligosaccharide microcapsules, 60 parts of honeysuckle, 40 parts of chrysanthemum, 40 parts of wolfberry fruit, 40 parts of astragalus membranaceus, 40 parts of dendrobium, 40 parts of mint, 40 parts of radix ophiopogonis, 60 parts of white granulated sugar, 30 parts of xylitol, 0.5 part of pigment, 0.5 part of essence, 30 parts of concentrated fruit juice and 20 parts of gili tablet.

A preparation method of an anti-caries health-care candy comprises the following steps:

s1 preparation of apple polyphenol-chitosan oligosaccharide microcapsules

Sufficiently dissolving apple polyphenol and chitosan oligosaccharide according to the mass ratio of 1:3, and performing spray drying to obtain the apple polyphenol-chitosan oligosaccharide microcapsule, wherein the spray drying parameters are as follows: feed rate 130m³The air inlet temperature is 150 ℃, and the feeding rate is 260 mL/h;

s2, pretreatment of raw materials

Adding apple polyphenol-chitosan oligosaccharide microcapsules, honeysuckle, chrysanthemum, wolfberry fruit, astragalus, dendrobium, mint and radix ophiopogonis into water with the temperature of 60 ℃ according to the weight ratio of 1:8, and soaking for 1h to obtain a pretreated raw material;

s3, decocting the raw materials

Adding water into the raw material pretreated by the step S2, decocting, and filtering to obtain a raw material solution;

s4 preparation of anti-caries health candy

Adding the white granulated sugar with the formula amount into the raw material liquid obtained in the step S3, dissolving the sugar, converting the dissolved sugar into massecuite, adding the xylitol, the pigment, the essence, the concentrated fruit juice and the gilding tablet into the massecuite according to the formula amount when the viscosity of the massecuite is 2500cP and the temperature is 80 ℃, dispersing uniformly to obtain a mixed sugar body, carrying out injection molding on the mixed sugar body, and cooling to obtain the anti-dental caries health-care candy.

Example 3

An anti-caries health-care candy is composed of the following raw materials in parts by weight: 10 parts of apple polyphenol-chitosan oligosaccharide microcapsules, 45 parts of honeysuckle, 30 parts of chrysanthemum, 30 parts of wolfberry fruits, 30 parts of astragalus membranaceus, 30 parts of dendrobium, 30 parts of mint, 30 parts of radix ophiopogonis, 40 parts of white granulated sugar, 20 parts of xylitol, 0.3 part of pigment, 0.3 part of essence, 20 parts of concentrated fruit juice and 15 parts of gili tablet.

A preparation method of an anti-caries health-care candy comprises the following steps:

s1 preparation of apple polyphenol-chitosan oligosaccharide microcapsules

Sufficiently dissolving apple polyphenol and chitosan oligosaccharide according to the mass ratio of 1:2.5, and performing spray drying to obtain the apple polyphenol-chitosan oligosaccharide microcapsule, wherein the spray drying parameters are as follows: the feed rate was 120m³The air inlet temperature is 140 ℃, and the feeding rate is 250 mL/h;

s2, pretreatment of raw materials

Adding apple polyphenol-chitosan oligosaccharide microcapsules, honeysuckle, chrysanthemum, wolfberry fruit, astragalus, dendrobium, mint and radix ophiopogonis into water with the temperature of 55 ℃ according to the weight ratio of 1:7.5, and soaking for 45min to obtain a pretreated raw material;

s3, decocting the raw materials

Adding water into the raw material pretreated by the step S2, decocting, and filtering to obtain a raw material solution;

s4 preparation of anti-caries health candy

Adding the white granulated sugar with the formula amount into the raw material liquid obtained in the step S3, dissolving the sugar, converting the dissolved sugar into massecuite, adding the xylitol, the pigment, the essence, the concentrated fruit juice and the gilding into the massecuite according to the formula amount when the viscosity of the massecuite is 2200cP and the temperature is 65 ℃, dispersing uniformly to obtain a mixed sugar body, carrying out injection molding on the mixed sugar body, and cooling to obtain the anti-dental caries health-care candy.

In order to further illustrate the effect of the invention, the invention is also provided with a comparative example which is concretely as follows:

comparative example 1

An anti-caries health-care candy is composed of the following raw materials in parts by weight: 45 parts of honeysuckle, 30 parts of chrysanthemum, 30 parts of medlar, 30 parts of astragalus, 30 parts of dendrobium, 30 parts of mint, 30 parts of radix ophiopogonis, 40 parts of white granulated sugar, 20 parts of xylitol, 0.3 part of pigment, 0.3 part of essence, 20 parts of concentrated fruit juice and 15 parts of Jilibing tablets.

A preparation method of an anti-caries health-care candy comprises the following steps:

s1 preparation of apple polyphenol-chitosan oligosaccharide microcapsule

S2, pretreatment of raw materials

Adding honeysuckle, chrysanthemum, medlar, astragalus, dendrobium, mint and radix ophiopogonis into water with the temperature of 55 ℃ according to the weight ratio of 1:7.5 according to the formula amount, and soaking for 45min to obtain a pretreated raw material;

s3, decocting the raw materials

Adding water into the raw material pretreated by the step S2, decocting, and filtering to obtain a raw material solution;

s4 preparation of anti-caries health candy

Adding the white granulated sugar with the formula amount into the raw material liquid obtained in the step S3, dissolving the sugar, converting the dissolved sugar into massecuite, adding the xylitol, the pigment, the essence, the concentrated fruit juice and the gilding into the massecuite according to the formula amount when the viscosity of the massecuite is 2200cP and the temperature is 65 ℃, dispersing uniformly to obtain a mixed sugar body, carrying out injection molding on the mixed sugar body, and cooling to obtain the anti-dental caries health-care candy.

The results of the examination of the minimum inhibitory concentration, the inhibition of glucosyltransferase activity, the inhibition of bacterial adhesion and the inhibition of the formation of bacterial biofilm on the dental surface of the anti-caries health candies prepared in examples 1 to 3 and comparative example 1 are shown in table 1 below:

TABLE 1 antibacterial Condition of caries-resistant health candy

As can be seen from Table 1: examples 1 to 3 compared with comparative example 1, the caries resistant healthy candies prepared in examples 1 to 3 had a minimum inhibitory concentration of 7.1mg/ml against Streptococcus mutans, an inhibitory concentration of 2.2mg/ml against glucosyltransferase activity, an inhibitory concentration of 1.9mg/ml against bacterial adhesion, and an inhibitory concentration of 1.1mg/ml against bacterial biofilm formation on the dental surface; the caries resistant health-care candy prepared in the comparative example 1 has weak bacteriostatic performance, and the reasons are that: compared with the comparative example 1, the apple polyphenol-chitosan oligosaccharide microcapsule is added into the formula of the examples 1-3, the apple polyphenol and the chitosan oligosaccharide can form the apple polyphenol-chitosan oligosaccharide microcapsule in a van der Waals force and intermolecular hydrogen bond mode, and the substance has the functional groups of the apple polyphenol and the chitosan oligosaccharide and synergistically enhances the bacteriostatic activity of the anti-caries health-care candy.

It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.