阅读说明：本技术 一种鲀胶原低聚肽粉的制作方法 (Method for preparing puffer fish collagen oligopeptide powder ) 是由 罗士柱 于 2019-04-15 设计创作，主要内容包括：本发明公开了一种鲀胶原低聚肽粉的制作方法,包括鲀皮清洗、提胶、酶解、灭酶、超滤、干燥制粉,其特征在于所述酶解过程使用复配内切酶,所述复配内切酶由木瓜蛋白酶、中性蛋白酶、碱性蛋白酶组成。本发明采用木瓜蛋白酶、中性蛋白酶、碱性蛋白酶组成的复配内切酶进行酶解,多点位切割,制得的鲀胶原低聚肽粉中低聚肽含量高达97.2％,易于被小肠、人体皮肤等直接吸收,减少了营养成分的浪费。(The invention discloses a method for preparing puffer fish collagen oligopeptide powder, which comprises the steps of washing puffer fish skin, extracting gelatin, carrying out enzymolysis, inactivating enzyme, carrying out ultrafiltration, and drying to prepare powder. The invention adopts the compound incision enzyme composed of papain, neutral protease and alkaline protease to carry out enzymolysis and multi-point cutting, the content of the oligopeptide in the prepared fugu collagen oligopeptide powder is as high as 97.2 percent, the fugu collagen oligopeptide powder is easy to be directly absorbed by small intestines, human skin and the like, and the waste of nutrient components is reduced.)

1. A method for preparing takifugu collagen oligopeptide powder comprises the steps of takifugu skin cleaning, gum extraction, enzymolysis, enzyme deactivation, ultrafiltration and drying for powder preparation, and is characterized in that compound incision enzyme is used in the enzymolysis process, and the compound incision enzyme consists of papain, neutral protease and alkaline protease.

2. The method for preparing the takifugu collagen oligopeptide powder according to claim 1, wherein the mass ratio of the papain, the neutral protease and the alkaline protease is 1:1: 1.

3. The method for preparing the takifugu collagen oligopeptide powder according to claim 1 or 2, which is characterized by comprising the following steps of:

s1, cleaning: washing the skin of the fugu rubripes with clear water;

s2, isolating and extracting gel by a water bath heating method: putting the takifugu flavidus skin obtained in the step S1 into a screen bag, lining the screen bag on a water bath temperature adjusting barrel, adjusting the temperature to 100 ℃, keeping for 5 hours, gradually cooling, taking out the screen bag, removing residues of the takifugu flavidus skin in the screen, and obtaining takifugu flavidus liquid;

s3, enzymolysis: keeping the temperature of the fugu rubber solution obtained in the step S2 at 50-55 ℃, adding compound endonuclease, uniformly stirring, and performing enzymolysis for 30 minutes;

s4, enzyme deactivation: heating the zymolyte obtained in the step S3 to 85 ℃, and keeping for 20 minutes to inactivate enzyme;

s5, ultrafiltration: diluting the enzyme-inactivated zymolyte obtained in the step S4 with pure water to the original concentration of 1/3 or lower, wherein the temperature in the whole filtering process is lower than the temperature required by an ultrafiltration membrane, and performing ultrafiltration to obtain a clarified fugu collagen oligopeptide liquid;

s6, drying to prepare powder: and (4) drying the fugu collagen oligopeptide liquid obtained in the step (S5) at a low temperature until the water content is lower than 4%, and crushing to obtain the fugu collagen oligomerization powder.

4. The method for preparing the takifugu collagen oligopeptide powder according to claim 3, wherein the addition amount of the compound endonuclease in the step S3 is 0.3-0.5% of the weight of the substrate.

5. The method of claim 3, wherein the step S2 comprises a water bath temperature control tank comprising an inner tank, an outer tank, a heating pipe, and a temperature control device, wherein the inner tank is lined with a mesh bag, and water is added between the outer tank and the inner tank to heat the contents of the inner tank.

6. The method for preparing puffer fish collagen oligopeptide powder according to claim 3, wherein the sieve bag used in step S2 is an 80-mesh high temperature resistant food grade nylon monofilament filter bag.

7. The method for preparing puffer fish collagen oligopeptide powder according to claim 3, wherein the ultrafiltration membrane is required to have a temperature of less than 70 ℃.

8. The method for producing puffer fish collagen oligopeptide powder according to any one of claims 3 to 7, wherein the puffer fish skin is derived from Fugu rubripes or Fugu rhabdomyoides.

9. A puffer fish collagen oligopeptide powder, which is prepared by the method for preparing the puffer fish collagen oligopeptide powder according to any one of claims 3 to 7.

Technical Field

The invention relates to the technical field of oligopeptide preparation, in particular to a method for preparing takifugu collagen oligopeptide powder.

Background

The collagen has wide molecular weight distribution, and the collagen with large molecular weight is difficult to be absorbed and utilized by human bodies, and is suitable for stabilizing agents, emulsifying agents, clarifying agents and the like. The collagen with smaller molecular weight is less than 1000 daltons, and the collagen is also called collagen oligopeptide, so that the collagen is easy to absorb by a human body due to the smaller molecular weight, is more applied to nutritional health-care food, and has higher demand on the market of the collagen oligopeptide. Among collagen oligopeptides with relatively small molecular weight, the fish collagen oligopeptides are deep-processed products of fish, which are prepared by refining raw materials of fish skin and bone which are free of pollution or have relatively small pollution, most of the collagen oligopeptides are small molecular mixed peptides which are composed of 2-8 amino acids and have molecular weight of 200-. Puffer fish is a marine fish, can expand a body by several times when being stimulated, is rich in elastic collagen in skin, is easy to absorb by a human body, and has high nutritional value, but the puffer fish collagen oligopeptide with moderate molecular weight obtained in the prior art has the problems of impurity separation, enzyme selection and processing technology.

Disclosure of Invention

In view of the above, the technical problem to be solved by the present invention is to provide a method for producing puffer fish collagen oligopeptide powder, which realizes batch production of puffer fish collagen oligopeptide powder with low cost and high purity.

The invention adopts the specific technical scheme that:

a method for preparing takifugu collagen oligopeptide powder comprises the steps of takifugu skin cleaning, gum extraction, enzymolysis, enzyme deactivation, ultrafiltration and drying for powder preparation, and is characterized in that compound incision enzyme is used in the enzymolysis process, and the compound incision enzyme consists of papain, neutral protease and alkaline protease.

In order to better realize the invention, the mass ratio of the papain to the neutral protease to the alkaline protease is 1:1: 1.

The manufacturing method of the takifugu collagen oligopeptide powder comprises the following specific steps:

s1, cleaning: washing the skin of the fugu rubripes with clear water;

s2, isolating and extracting gel by a water bath heating method: putting the takifugu flavidus skin obtained in the step S1 into a screen bag, lining the screen bag on a water bath temperature adjusting barrel, adjusting the temperature to 100 ℃, keeping for 5 hours, gradually cooling, taking out the screen bag, removing residues of the takifugu flavidus skin in the screen, and obtaining takifugu flavidus liquid;

s3, enzymolysis: keeping the temperature of the fugu rubber solution obtained in the step S2 at 50-55 ℃, adding compound endonuclease, uniformly stirring, and performing enzymolysis for 30 minutes;

s4, enzyme deactivation: heating the zymolyte obtained in the step S3 to 85 ℃, and keeping for 20 minutes to inactivate enzyme;

s5, ultrafiltration: diluting the enzyme-inactivated zymolyte obtained in the step S4 with pure water to the original concentration of 1/3 or lower, wherein the temperature in the whole filtering process is lower than the temperature required by an ultrafiltration membrane, and performing ultrafiltration to obtain a clarified fugu collagen oligopeptide liquid;

s6, drying to prepare powder: and (4) drying the fugu collagen oligopeptide liquid obtained in the step (S5) at a low temperature until the water content is lower than 4%, and crushing to obtain the fugu collagen oligomerization powder.

In order to better realize the method, the addition amount of the compound endonuclease in the step S3 is 0.3-0.5% of the weight of the substrate.

In order to better implement the present invention, the water bath temperature adjusting barrel mentioned in step S2 comprises an inner barrel, an outer barrel, a heating pipe and a temperature adjusting device, wherein the inner barrel is used for lining a mesh bag, and water is added between the outer barrel and the inner barrel, so that the content in the inner barrel can be heated in a water-proof manner.

In order to better implement the invention, step S2 mentions that the screen bag used is an 80-mesh high-temperature-resistant food-grade nylon monofilament filter bag.

In order to better implement the present invention, the ultrafiltration membrane requires a temperature of less than 70 ℃.

In order to better implement the present invention, said takifugu skin is derived from takifugu rubripes or takifugu rhabdomyoides.

Correspondingly, the invention also provides the takifugu collagen oligopeptide powder, which is prepared by the preparation method of the takifugu collagen oligopeptide powder.

Compared with the prior art, the invention has the following beneficial effects:

1. the invention adopts the compound incision enzyme composed of papain, neutral protease and alkaline protease to carry out enzymolysis and multi-point cutting, the content of oligopeptide in the prepared fugu collagen oligopeptide powder is as high as 97.2 percent, the fugu collagen oligopeptide powder is easy to be directly absorbed by small intestines, human skin and the like, and the waste of nutrient components is reduced;

2. the invention adopts a water bath heating method to isolate and extract glue, adopts high temperature of 100 ℃ and keeps heating for 5 hours, can effectively remove fishy smell while extracting glue, and improves the quality of the takifugu collagen oligopeptide product;

3. in the preparation process, the water bath heating method is adopted for isolation and extraction of glue, ultrafiltration membrane filtration and other methods, so that the prepared fugu collagen oligopeptide powder has high purity;

4. the invention strictly controls the selection of enzyme and each step, and the prepared fugu collagen oligopeptide has high purity, good quality, simple and easy operation of the preparation method and low cost.

Detailed Description

The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto, and various substitutions and alterations can be made without departing from the technical idea of the present invention as described above, according to the common technical knowledge and the conventional means in the field.