发酵工艺
阅读说明：本技术 发酵工艺 (Fermentation process ) 是由 菲利普·加班特 ***·尔·巴克库里 劳伦塞·范·梅尔德伦 于 2018-12-19 设计创作，主要内容包括：一些实施方式涉及使用包含第一核酸序列的自复制染色体外核酸分子、用微生物宿主生产目标产物的方法,该第一核酸序列的遗传活性向宿主赋予优势,可选地其中所述第一核酸分子的遗传活性是受控的。(Some embodiments relate to a method of producing a product of interest with a microbial host using a self-replicating extra-chromosomal nucleic acid molecule comprising a first nucleic acid sequence whose genetic activity confers a advantage to the host, optionally wherein the genetic activity of the first nucleic acid molecule is controlled.)
技术领域
本文的实施方式涉及一种使用自复制染色体外核酸分子(auto-replicativeextra-chromosomal nucleic acid molecule)、用微生物宿主生产目标产物的方法，该自复制染色体外核酸分子包含第一核酸序列，该第一核酸序列的遗传活性向宿主赋予优势，可选地其中所述第一核酸分子的遗传活性是受控的。
背景技术
抗生素被广泛用作在微生物细胞中产生目标产物的选择剂。然而，存在与使用抗生素相关的诸多缺点，诸如抗生素在环境中的大规模传播。另外，在DNA构建体中编码抗生素抗性的序列代表了细胞的能量负担，并因此对产物的产量产生负面影响。当赋予抗性的基因是大基因，当其以高水平表达和/或其被组成型表达时，这种能量负担特别重要。
因此，仍然需要一种替代的且甚至改进的方法，其不具有现有方法的所有缺点。
发明内容
在第一方面，提供了一种用微生物宿主生产目标产物的方法，所述方法包括以下步骤：
a)提供包含自复制染色体外核酸分子的微生物宿主，该自复制染色体外核酸分子包含第一核酸序列，可选地其中所述第一核酸序列的遗传活性是受控的；
b)可选地，自复制染色体外核酸分子包含参与所述目标产物生产的第二核酸序列，其中所述第二核酸序列的遗传活性独立于所述第一序列而受控制；
c)在允许所述微生物宿主将所述第一核酸序列表达至给定水平以将自复制染色体外分子维持在生长的微生物群体中并同时遗传控制编码所述目标产物的第二序列的条件下，培养所述微生物宿主。
步骤a)
步骤a)包含提供微生物宿主，其包含含有第一核酸序列的自复制染色体外核酸分子，该第一核酸序列的遗传活性赋予宿主以优势，可选地其中，所述第一核酸序列的遗传活性是受控的。自复制染色体外核酸分子可以提供在微生物宿主中(例如，如本文所述的微生物细胞)。例如，宿主或宿主的前身可以事先已使用自复制染色体外核酸分子进行转化。因此，在一些实施方式中，步骤a)包含提供一种微生物细胞宿主，其包含含有第一核酸序列的自复制染色体外核酸分子，该第一核酸序列的遗传活性赋予宿主以优势，可选地其中所述第一核酸序列的遗传活性是受控的。
可选的转化步骤
在一些实施方式中，在仅允许已经接收了自复制染色体外核酸分子的宿主存活的条件下，使用自复制染色体外核酸分子转化微生物宿主，由此提供包含自复制染色体外核酸分子的微生物宿主。由此，在一些实施方式中，所述方法进一步包含，在步骤a)之前或期间，在仅允许已接收自复制染色体外核酸分子的宿主存活的条件下，使用自复制染色体外核酸分子转化微生物宿主，由此提供包含自复制染色体外核酸分子的微生物宿主。
转化到微生物宿主中的自复制染色体外核酸分子可选地包含步骤b)的第二核酸序列。随后，可以根据步骤c)培养包含自复制染色体外核酸分子的微生物宿主。
在本文实施方式的方法、用途、组合物、宿主和核酸的背景下，提供了一种包含第一核酸序列的自复制染色体外核酸分子。自复制染色体外核酸分子可以不存在基因组而存在，并且可以衍生自或包含质粒或附加体、微染色体等，或者基本上由或由质粒或附加体、微染色体等组成。该特征是有吸引力的，因为可以将更高数量(取决于所使用的质粒，从一到数百个拷贝或从10到50个拷贝)这种核酸分子的拷贝引入并维持在微生物细胞宿主中。此外，在本文实施方式的方法中可以使用任何宿主。在一些实施方式中，无需修改宿主的基因组。自复制染色体外核酸分子中存在执行本文实施方式的方法所需的遗传元件。此种自复制染色体外核酸分子通常包含复制起点、作为目标的第一核酸序列和调控区。在一些实施方式中，不受理论限制，编码免疫调节剂的第一核酸序列充当可选择的标志物，以维持宿主细胞中自复制染色体外核酸的存在和功能。在一些实施方式中，编码免疫调节剂的第一核酸序列维持自复制染色体外核酸的存在，以便可以生产产物。该产物可以改变宿主所在的环境，例如，通过发酵环境中的物质以产生一种或多种新物质。在一些实施方式中，通过提供针对已经获得突变且不生产功能性免疫调节剂、生产功能降低的免疫调节剂和/或与尚未获得突变的自复制染色体外核酸相比产生较低水平免疫调节剂的自复制染色体外核酸的选择性压力，遗传漂变被最小化。
在本文实施方式的方法、用途、组合物、宿主和核酸的背景下，由所述第一核酸序列的第一核酸分子能够表现出遗传活性，所述遗传活性赋予其中存在所述第一核酸分子并且表达这种遗传活性的微生物宿主以选择优势。该遗传活性是由第一核酸分子编码的产物提供的。此外，这种遗传活性可以被控制或以低水平组成型表达，或者是可调节的，或者处于弱组成型启动子的控制之下。相信对所述活性的控制提供了限制宿主能量负担的优点。类似地，当遗传活性以低水平组成型表达或可调节或在弱组成型启动子的控制下时，可以获得限制宿主能量负担的优势。在整个申请文本中，“赋予优势”的概念可以由“赋予对细菌素的免疫力”或“赋予对细菌素的抗性”代替。在一些实施方式中，所述第一核酸序列编码本文所述的免疫调节剂，并由此给宿主赋予优势。
第二核酸序列直接或间接编码目标产物。相同的描述适用于本文所述的第二核酸分子的遗传活性。在一些实施方式中，目标产物包含可用于工业过程(例如发酵工艺)的酶。发酵工艺可以在培养基中发酵至少一种化合物。在一些实施方式中，目标产物包含工业上有用的分子，例如糖类、脂质、有机分子、营养物、肥料、生物燃料、化妆品(或其前体)、药物或生物制药产品(或其前体)、或者所列项目中的两种或更多种。
在本文实施方式的方法、用途、组合物、宿主和核酸的背景下，遗传活性可以是指由微生物宿主中所述第一核酸分子的存在引起的或与之相关的任何活性。所述活性的优点可以是在给定条件下(pH、温度、给定分子(诸如细菌素或两种或多种如本文所述细菌素的组合)的存在…)能够存活或者存活并生长的能力。因此，可以通过确定包含自复制染色体外核酸分子的微生物细胞/宿主的数量来评估所述活性的优势。可以在所述可选的转化步骤结束时和/或期间(但在培养步骤c)之前，或在步骤a)和培养步骤c)之前)和/或在培养步骤c)之前进行评估。在一个实施方式中，当在允许已经接收自复制染色体外核酸分子的微生物宿主存活的条件下(例如，通过针对如本文所述且在所述给定条件下存在的一种或多种细菌素具有免疫力)培养细胞时，与初始微生物细胞/宿主的数量相比，包含所存在的自复制染色体外核酸分子的微生物宿主细胞的数量未减少并且可以增加至少5％、至少10％、至少15％、至少20％、至少25％、至少30％、至少35％、至少40％、至少45％、至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少95％或更多。该评估步骤可以持续至少6小时、12小时、24小时、36小时、48小时、60小时、72小时、84小时、96小时、108小时、120小时或更长，包括任何两个列出值之间的范围。
在本文实施方式的方法、用途、组合物、宿主和核酸的背景下，遗传活性的控制可以是指核酸分子(即第一和/或第二核酸分子)活性的增加或减少。因此，遗传活性的控制可以被控制或以低水平组成型表达，或者是可调节的，或者处于弱组成型启动子的控制之下。在一些实施方式中，被调节/控制遗传活性的编码产物包含免疫调节剂、基本上由免疫调节剂组成或由免疫调节剂组成，并且参与了目标产物的生产。在一些实施方式中，遗传活性在基因表达水平上受到调节/控制。在一些实施方式中，遗传活性在转录水平上受到调节，例如通过激活或抑制启动子。在一些实施方式中，在这种情况下，启动子是诱导型启动子。在一些实施方式中，在这种情况下，启动子是弱启动子。不受理论的限制，一些实施方式的弱启动子可以被修改为上调或下调转录水平，由此赋予宿主的优势(例如免疫调节剂活性)对在启动子控制下的基因产物的水平和/或活性的变化敏感。在一些实施方式中，所述启动子包含由SEQ ID NO:707表示的P24启动子和/或由SEQ ID NO:708表示的ProC启动子和/或P24 LacO杂交启动子，由SEQ ID NO:707表示的P24启动子和/或由SEQ ID NO:708表示的ProC启动子和/或P24 LacO杂交启动子组成，或基本上由SEQ ID NO:707表示的P24启动子和/或由SEQ ID NO:708表示的ProC启动子和/或P24 LacO杂交启动子组成。P24 LacO杂交启动子是可调/受控的启动子。在一些实施方式中，基因活性在转录后水平上受到调节/控制，例如通过调节RNA的稳定性。在一些实施方式中，在翻译水平上调节/控制遗传活性，例如通过调节翻译的开始。在一些实施方式中，在翻译后水平上调节/控制遗传活性，例如通过调节多肽的稳定性、对多肽进行翻译后修饰或将抑制剂与多肽结合。
在一些实施方式中，提高了遗传活性。在一些实施方式中，提高了参与生产目标产物的免疫调节剂和/或第二核酸分子的编码产物中的至少一个的活性。从概念上讲，可以通过直接激活遗传活性，或通过降低遗传活性抑制剂的活性，来提高遗传活性。在一些实施方式中，通过下列中的至少一种来激活遗传活性：诱导启动子活性，抑制转录阻遏物，增加RNA稳定性，抑制转录后抑制剂(例如，抑制核酶或反义寡核苷酸)，诱导翻译(例如，通过可调节的tRNA)，进行所需的翻译后修饰，或抑制翻译后抑制剂(例如针对由该基因编码的多肽的蛋白酶)。在一些实施方式中，存在于所需环境中的化合物诱导启动子。例如，培养基中铁的存在可通过如本文所述的铁敏感启动子诱导转录。在一些实施方式中，存在于所需培养基中的化合物抑制转录阻遏物。例如，环境中四环素的存在可以抑制tet阻遏物，从而允许tetO启动子的活性。在一些实施方式中，仅在所需培养基外发现的化合物诱导转录。
在一些实施方式中，降低了遗传活性。从概念上讲，可以通过直接抑制遗传活性，或通过降低遗传活性激活剂的活性，来降低遗传活性。在一些实施方式中，遗传活性降低，但仍保持一定水平的活性。在一些实施方式中，遗传活性被完全抑制。在一些实施方式中，通过抑制启动子活性、激活转录阻遏物、降低RNA稳定性、激活转录后抑制剂(例如，表达核酶或反义寡核苷酸)、抑制翻译(例如，通过可调节的tRNA)、无法进行所需的翻译后修饰、灭活多肽(例如通过结合抑制剂或通过多肽特异性蛋白酶)、或无法正确定位多肽中的至少一种来降低遗传活性。在一些实施方式中，通过从所需位置去除基因(例如通过使用FLP-FRT或cre-lox盒切除基因)、同源重组或CRIPR-CAS9活性、或通过质粒的丢失或降解来降低遗传活性。在一些实施方式中，基因产物(例如多肽)或由基因产物产生的产物(例如酶促反应的产物)进一步抑制基因活性(例如负反馈回路)。
在一些实施方式中，由所述第一核酸分子的遗传活性赋予给微生物宿主的优势是在包含细菌素(或细菌素混合物)的培养基中存活或者存活并生长的能力。如本文所用，“细菌素”涵盖了这种多肽的无细胞或化学合成版本。“细菌素”及其词根的变化形式，也可以是指已由宿主细胞分泌的多肽。因此，细菌素涵盖由细菌产生用以抑制相似或密切相关细菌菌株生长的蛋白质毒素。它们与酵母和草履虫的杀伤因子相似，并且在结构、功能和生态上都多样化。细菌素还涵盖由宿主细胞分泌的细菌素的合成变体。细菌素的合成变体可以以任何方式源自由宿主细胞分泌的细菌素，只要所述合成变体仍然表现出由宿主细胞分泌的相应细菌素的活性的至少10％、20％、30％、40％、50％、60％、70％、80％、90％即可。说明书末尾的一般描述部分中提供了抗生素的详细说明。
“细菌素”可以中和除了生成多肽的单个宿主细胞以外的至少一个细胞，包括与宿主细胞克隆相关的细胞和其他微生物细胞。
表达特定“免疫调节剂”(本文更详细地讨论)的细胞对特定细菌素或一组细菌素的中和作用具有免疫力。如此，细菌素可以中和产生细菌素的细胞和/或其他微生物细胞，只要这些细胞不产生适当的免疫调节剂。如此，细菌素可以对多种其他微生物发挥细胞毒性或抑制生长的作用。在一个实施方式中，细菌素是由微生物细胞的翻译机制(例如核糖体等)产生的。在另一个实施方式中，细菌素是化学合成的。一些细菌素可以衍生自多肽前体。多肽前体可以经历裂解(例如通过蛋白酶处理)以产生细菌素本身的多肽。如此，在一些实施方式中，细菌素由前体多肽产生。在一些实施方式中，细菌素包含经过翻译后修饰(例如裂解或添加一个或多个官能团)的多肽，基本上由该多肽组成或由该多肽组成。
细菌素的中和活性可以包括阻止微生物繁殖或细胞毒性。一些细菌素具有细胞毒活性(例如“杀菌剂”作用)，并由此可以杀灭微生物，例如细菌、酵母、藻类、合成微生物等。一些细菌素可以抑制微生物(例如细菌、酵母、藻类、合成微生物等)的繁殖(例如“抑菌”作用)，例如通过阻止细胞周期。
已经鉴定并表征了许多细菌素(见表1.1和表1.2.)。不受任何特定理论的限制，示例性的细菌素可以被归类为“I类”细菌素，其通常会经历翻译后修饰，和“II类”细菌素，其通常未经修饰。此外，每个类别中的示例性细菌素可以分为不同的亚组，如表1.1所示，其改编自Cotter,P.D.et al."Bacteriocins-a viable alternative to antibiotics"NatureReviews Microbiology 11:95-105，在此通过引用以其整体并入。
不受任何特定理论的限制，细菌素可以通过多种方式中和目标微生物细胞。例如，细菌素可渗透细胞壁，从而使细胞壁去极化并干扰呼吸。表1.1：示例性细菌素的分类。
表1.1：示例性细菌素的分类
根据本文实施方式，可以使用许多细菌素。表1.2示出了示例性的细菌素。在一些实施方式中，提供了包含表1.2的多肽序列的、，基本上由表1.2的多肽序列组成的或由表1.2的多肽序列组成的至少一种细菌素。如表1.2所示，一些细菌素作为分子对起作用。如此，可以理解的是，除非另有明确说明，当在本文中讨论功能性“细菌素”或“提供细菌素”等时，包括功能性细菌素对以及可单独起作用的细菌素。参考表1.2，括号中列出的“起源生物”指示已知会产生所示细菌素的生物的替代名称和/或菌株信息。
本文的实施方式还包括与表1.2中所述细菌素具有同一性的肽和蛋白质。术语“同一性”是指包括核酸或蛋白质序列同源性或三维同源性。存在多种技术来确定与多肽的核酸或多肽序列同源性和/或三维同源性。通常使用这些方法来发现一个序列、结构域或模型与目标序列、结构域或模型的同一性程度。种类繁多的功能性细菌素可以结合本文公开的细菌素的特征，由此提供相对于表1.2中细菌素的各种程度的同一性。在一些实施方式中，细菌素与表1.2中的任一多肽具有至少50％的同一性，例如至少50％、51％、52％、53％、54％、55％、56％、57％、58％、59％、60％、61％、62％、63％、64％、65％、66％、67％、68％、69％、70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或100％的同一性。可以使用BLAST软件(Altschul,S.F.,et al.(1990)"Basic localalignment search tool."J.Mol.Biol.215:403-410，可从以下网址访问万维网：blast.ncbi.nlm.nih.gov)以默认参数来确定同一性百分比。
虽然表1.2中的细菌素是天然存在的，但本领域技术人员可以理解的是，根据本文的一些实施方式可以使用表1.2细菌素的变体、表1.2细菌素以外的天然存在的细菌素或其变体、或合成细菌素。在一些实施方式中，相对于野生型蛋白质，此类变体对相同或不同的微生物或微生物物种具有增强或降低水平的细胞毒性或生长抑制活性。多个基序已经被公认为是细菌素的特征。例如，基序YGXGV(SEQ ID NO:2)(其中X是任何氨基酸残基)是Ila类细菌素特征性的N末端共有序列。因此，在一些实施方式中，合成细菌素包含N末端序列，该N末端序列与SEQ ID NO:2具有至少50％的同一性，例如与SEQ ID NO:2具有至少50％、51％、52％、53％、54％、55％、56％、57％、58％、59％、60％、61％、62％、63％、64％、65％、66％、67％、68％、69％、70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或100％的同一性。在一些实施方式中，合成细菌素包含N末端序列，该N末端序列包含SEQ ID NO:2。此外，一些lib类细菌素包含GxxxG基序(x指任何氨基酸)。不受任何特定理论的限制，可以相信的是，GxxxG基序可以介导细胞膜中螺旋蛋白之间的缔合，例如通过细胞膜相互作用以促进细菌素介导的中和。如此，在一些实施方式中，细菌素包含促进与细胞膜相互作用的基序。在一些实施方式中，细菌素包含GxxxG基序。可选地，包含GxxxG基序的细菌素可以包含螺旋结构。除了本文所述的结构之外，如本文所用的“细菌素”还包括对微生物细胞具有与本文明确提供的任何细菌素基本相同的作用的结构。
已经证明，与任何个体细菌素相比，包含两种或更多种细菌素或其部分的融合多肽、基本上由两种或更多种细菌素或其部分组成的融合多肽或者由两种或更多种细菌素或其部分组成的融合多肽，可以具有针对范围更广的微生物的中和活性。例如，已经证明，杂合细菌素Ent35-MccV(GKYYGNGVSCNKKGCSVDWGRAIGIIGNNSAANLATGGAAGWKSGGGASGRDIAMAIGTLSGQFVAGGIGAAAGGVAGGAIYDYASTHKPNPAMSPSGLGGTIKQKPEGIPSEAWNYAAGRLCNWSPNNLSDVCL,SEQ ID NO:3)对病原性革兰氏阳性和革兰氏阴性细菌具有抗菌活性(Acuna et al.(2012),FEBS Open Bio,2:12-19)。值得注意的是，Ent35-MccV融合细菌素从N末端到C末端包含N末端甘氨酸、肠道菌素CRL35、包含三个甘氨酸的连接子和C末端小菌素V。在本文中预期的是，细菌素可以包含两种或更多种具有细菌素活性的多肽的融合体。在一些实施方式中，提供了两种或更多种细菌素的融合多肽。在一些实施方式中，所述两种或更多种细菌素包含表1.2中的多肽或其修饰物，基本上由表1.2中的多肽或其修饰物组成，或由表1.2中的多肽或其修饰物组成。在一些实施方式中，包含两种或更多种细菌素的融合多肽比任何单个细菌素都具有更广谱的活性，例如具有针对更多微生物的中和活性、在更广泛环境条件下的中和活性和/或更高效的中和活性。在一些实施方式中，提供了两种或更多种细菌素(例如二、三、四、五、六、七、八、九、或十种细菌素)的融合物。在一些实施方式中，两种或更多种细菌素多肽通过共价键(例如肽键)彼此融合。在一些实施方式中，连接子位于两个细菌素多太之间。在一些实施方式中，所述连接子包含一个或多个甘氨酸、基本上由一个或多个甘氨酸组成或由一个或多个甘氨酸组成，例如约1、2、3、4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19或20个甘氨酸。在一些实施方式中，连接子在细胞内被裂解以产生包含在融合蛋白中的单个细菌素。在一些实施方式中，修饰如本文提供的细菌素以相对于未经修饰的细菌素提供所需活性谱。例如，相对于未经修饰的细菌素，经修饰的细菌素针对相同的生物体可具有增强或降低的活性。替代地，针对未经修饰的细菌素具有较小活性或没有活性的生物体，经修饰的细菌素可具有增强的活性。
表1.2：示例性细菌素
例如，在一些实施方式中，需要抗真菌活性(诸如抗酵母活性)。已鉴定出许多具有抗真菌活性的细菌素。例如，已显示芽孢杆菌的细菌素对酵母菌株具有中和活性{参见Adetunji and Olaoye(2013)Malaysian Journal of Microbiology 9:130-13,在此通过引用以其全部内容并入)，粪肠球菌肽(WLPPAGLLGRCGRWFRPWLLWLQ SGAQY KWLGNLFGLGPK,SEQ ID NO:1)已经显示出对念珠菌具有中和活性{参见Shekh and Roy(2012)BMCMicrobiology 12:132,在此通过引用以其全部内容并入)，并且假单胞菌的细菌素已显示出对真菌(诸如新月弯孢菌、镰刀菌属种、蠕虫孢子属种和生物极地菌种)具有中和活性(Shalani and Srivastava(2008)The Internet Journal of Microbiology.Volume5Number 2.DOI:10.5580/27dd–可从以下网址访问万维网：archive,ispub.com/journal/the-internet-journal-of-micro bio logy/volume-5-number-2/screening-for-antifungal-activity-of-pseudomonas-flu orescens-against-phytopathogenic-fungi.html#sthash.d0Ys03UO.lDKuTlUS.dpuf,在此通过引用以其全部内容并入)。例如，来自5.枯草杆菌的杀葡孢菌素AJ1316{参见Zuber,P et al.(1993)PeptideAntibiotics.In Bacillus subtilis and Other Gram-Positive Bacteria:Biochemistry,Physiology,and Molecular Genetics ed Sonenshein et al.,pp.897-916,American Society for Microbiology,在此通过引用以其全部内容并入)和alirinBl{参见Shenin et al.(1995)Antibiot Khimioter 50:3-7,在此通过引用以其全部内容并入)被证明具有抗真菌活性。因此，在一些实施方式中，例如，需要中和真菌微生物的实施方式，细菌素包含杀葡孢菌素AJ1316或alirin B1之一。
例如，在一些实施方式中，需要蓝细菌培养物中的细菌素活性。在一些实施方式中，提供了细菌素来中和蓝细菌。在一些实施方式中，提供细菌素来中和通常在蓝细菌培养环境中发现的入侵微生物。已在各种各样的蓝细菌物种中鉴定出保守的细菌素多肽簇。例如，如Wang et al.((2011),Genome Mining Demonstrates the Widespread Occurrenceof Gene Clusters Encoding Bacteriocins in Cyanobacteria.PLoS ONE 6(7):e22384,在此通过引用以其全部内容并入)所报道的，已在至少43个蓝细菌物种中鉴定出至少145个推定的细菌素基因簇。示例性蓝细菌细菌素在表1.2中显示为SEQ ID NO:420、422、424、426、428、30、432、434、436、438、440、442、444、446、448和450。
在本文实施方式的方法、用途、组合物、宿主和核酸的背景下，尽管细菌素可能会通过如本文所述的不同机制作用于微生物细胞，但当用包含细菌素的培养基培养微生物宿主时，与初始微生物宿主的数量相比，当微生物宿主的数量减少了至少5％、至少10％、至少15％、至少20％、至少25％、至少30％、至少35％、至少40％、至少45％、至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少95％或更多时，所述细菌素可以说是具有活性的。通过计数存在的微生物宿主的数量来评估细菌素的活性之前，这一培养步骤可持续至少12小时、24小时、36小时、48小时、60小时、72小时或更长时间。可以通过在显微镜下计数细胞或通过任何已知的微生物技术来评估活性。在一些实施方式中，当用包含细菌素的培养基培养微生物宿主时，与微生物宿主的初始种群相比，当阻止至少指定数量或百分比的微生物宿主生长时，例如阻止至少5％、至少10％、至少15％、至少20％、至少25％、至少30％、至少35％、至少40％、至少45％、至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、或至少95％的所述微生物宿主，则所述细菌素是具有活性的。
在一些本文实施方式的方法、用途、组合物、宿主和核酸的背景下，细菌还给是分别由包含SEQ ID NO:198或200或由SEQ ID NO:198或200组成的氨基酸序列表示的B17或C7。根据本文的一些实施方式，已经通过实验证实B17和C7是易于产生、易于使用并且在培养基中稳定的选择剂(见实施例1)。本文实施方式的一些方法、用途、组合物、宿主和核酸还涵盖使用与SEQ ID NO:198或200具有至少50％同一性的细菌素，例如与SEQ ID NO:198或200具有至少50％、51％、52％、53％、54％、55％、56％、57％、58％、59％、60％、61％、62％、63％、64％、65％、66％、67％、68％、69％、70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或100％的同一性。B17或C7的此类变体可用于本文实施方式的方法、用途、组合物、宿主和核酸中，只要它们至少表现出B17或C7的实质活性即可。在此背景下，“实质”是指例如具有SEQ ID NO:198或200的B17或C7的活性的至少50％、至少60％、至少70％、至少80％、至少90％或至少100％或更高。细菌素的活性已在本文前面进行了描述。
在本文实施方式的方法、用途、组合物、宿主和核酸的背景下，并取决于目标微生物和所用的细菌素，技术人员将了解在培养基或琼脂培养皿中使用哪种浓度的细菌素。使用细菌素B17或C7，发明人能够制备包含所述细菌素的培养基，该培养基中所述细菌素的浓度使得能够实施本文实施方式的方法和用途，即观察或可视化所述遗传活性表达的优势。如果所述活性的优势是允许包含自复制染色体外核酸分子的宿主生长，那么当在允许已接收自复制染色体外核酸分子的微生物宿主生存并生长的条件下培养细胞时，所述培养基或琼脂平板中细菌素的数量已使得不包含自复制染色体外核酸分子的宿主的数量与初始微生物细胞/宿主的数量相比降低了至少5％、至少10％、至少15％、至少20％、至少25％、至少30％、至少35％、至少40％、至少45％、至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少95％或更多。该评估步骤的持续时间可以为至少6小时、12小时、24小时、36小时、48小时、60小时、72小时、84小时、96小时、108小时、120小时或更长时间。可以对所述培养基进行灭菌而不损失实质细菌素活性。在这一背景下，“实质”是指例如灭菌前培养基中存在的细菌素活性的多于50％，多于60％，多于70％，多于80％，多于90％。
适用于一些本文实施方式的方法、用途、组合物、宿主和核酸并且其产物提供了对细菌素的免疫力的第一核酸序列如表2所示。
表2：产物提供对细菌素的免疫力的示例性核酸序列
尽管对表2的细菌素提供免疫力的序列是天然存在的，但本领域技术人员将理解的是，根据本文的一些实施方式可以使用这些分子的变体、表2中所列分子以外的天然分子或合成分子。在一些实施方式中，特定分子或分子的特定组合赋予对特定细菌素、特定类别或种类的细菌素、或细菌素的特定组合的免疫力。表2列出了分子可以赋予免疫力的典型细菌素。虽然表2确定了赋予免疫力的分子的“起源生物”，但是根据本文的一些实施方式，赋予免疫力的这些分子可以容易地在其他天然存在的、经基因修饰的或合成的微生物中表达，以提供所需的细菌素免疫活性。因此，如本文使用的“免疫调节剂”或“赋予或提供对细菌素的免疫力的分子”不仅涵盖本文明确提供的结构，而且涵盖与本文所述的“免疫调节剂”结构具有基本相同作用的结构，包括全合成免疫调节剂，以及提供对在功能上与本文公开细菌素等同的细菌素的免疫力的免疫调节剂。
表2中示出了编码表2的多肽的示例性多核苷酸序列。本领域技术人员将容易理解，遗传密码是简并的，此外，密码子的使用可以基于表达基因产物的特定生物体而变化，因此，特定多肽可以由多于一个多核苷酸编码。在一些实施方式中，基于表达细菌素免疫调节剂的生物体的密码子使用来使用编码细菌素免疫调节剂的多核苷酸。在一些实施方式中，基于表达细菌素免疫调节剂的特定生物体来对编码细菌素免疫调节剂的多核苷酸进行密码子优化。广泛的功能性免疫调节剂可以结合本文公开的免疫调节剂的特征，由此提供相对于表2中免疫调节剂的广阔程度的同一性。在一些实施方式中，免疫调节剂与表2的任何一种多肽具有至少约50％的同一性，例如至少50％、51％、52％、53％、54％、55％、56％、57％、58％、59％、60％、61％、62％、63％、64％、65％、66％、67％、68％、69％、70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或100％的同一性。
在本文实施方式的方法、用途、组合物、宿主和核酸的背景下，对细菌素的抵抗性或免疫力可以是指在使用细菌素的培养步骤结束时尚未减少的微生物细胞数，并且在一些实施方式中，当用包含细菌素的培养基培养细胞时，与最初的微生物细胞数量相比，已经增加了至少5％、至少10％、至少15％、至少20％、至少25％、至少30％、至少35％、至少40％、至少45％、至少50％、至少55％、至少60％、至少65％、至少70％、至少75％、至少80％、至少85％、至少90％、至少95％或更多。在通过计数存在的微生物细胞的数量来评估细菌素的活性之前，这一培养步骤可以持续至少12小时、24小时、36小时、48小时、60小时、72小时、84小时、96小时、108小时、120小时或更长时间。
适用于一些本文实施方式的方法、用途、组合物、宿主和核酸并且编码产品赋予免疫力的核酸分子是McbG(对细菌素B17具有免疫力)，由SEQ ID NO:699表示。根据本文一些实施方式，已经通过实验证实McbG可组成性或诱导性地用作选择标志物(见实施例3)。另一适合的核酸是由SEQ ID NO:700表示的MccE(对细菌素C7具有免疫力)或由SEQ ID NO:701表示的其C末端部分。MccE已在对小菌素/细菌素敏感的菌株用作载体选择标志物(见实施例2)。一些实施方式的方法、用途、组合物、宿主和核酸还涵盖使用这样的核酸分子，其编码产物赋予对细菌素B17和/或C7的免疫力并且其与SEQ ID NO:699、700或701具有至少50％的同一性，例如与SEQ ID NO:699、700或701具有至少50％、51％、52％、53％、54％、55％、56％、57％、58％、59％、60％、61％、62％、63％、64％、65％、66％、67％、68％、69％、70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或100％的同一性。McbG和/或MccE的此类变体可以用于本文实施方式的方法、用途、组合物、宿主和核酸中，只要它们至少表现出McbG(分别地MccE)的实质活性即可。在此背景下，“实质”是指例如具有SEQ ID NO:699、700或701的McbG(分别地MccE)的活性的至少50％、至少60％、至少70％、至少80％、至少90％或至少100％或更多。本文先前已经描述了由McbG(分别地MccE)的编码产物赋予的免疫力。
令人惊讶地已经发现，由SEQ ID NO:701表示的MccE的C末端部分足以赋予对细菌素C7的抗性。在此背景下，部分是指例如原始核酸分子的至少50％、至少60％、至少70％、至少80％、至少90％或更多。这样的短核酸分子可以赋予对细菌素的抗性，这是非常有吸引力且令人惊讶的。可以预计，包含这样的短核酸分子的自复制染色体外核酸分子不会对微生物细胞造成任何负担。
用于本文一些实施方式的方法、用途、组合物、宿主和核酸并且其产物提供对细菌素的免疫力的进一步合适的核酸分子是单核酸分子，其单一产物提供对至少两种不同细菌素的免疫力。在一些实施方式中，此类核酸分子的此类产物提供了对B17和C7或对ColV和C7或对ColV和B17或对B17、C7和ColV的免疫力。编码ColV的核酸被鉴定为SEQ ID NO:65且相应的编码氨基酸序列被鉴定为SEQ ID NO:64。
在一些实施方式中，其产物提供对B17和C7的免疫力的核酸分子由与SEQ ID NO:715或716具有至少50％同一性的序列表示，例如与SEQ ID NO:715或716具有至少50％、51％、52％、53％、54％、55％、56％、57％、58％、59％、60％、61％、62％、63％、64％、65％、66％、67％、68％、69％、70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或100％的同一性。SEQ ID NO:715是与MccE融合的McbG的核酸分子。SEQ ID NO:716是融合到MccE的C末端部分的McbG的核酸分子，如本文先前所描述的。
在一些实施方式中，其产物提供对ColV和C7的免疫力的核酸分子由与SEQ ID NO:717或718具有至少50％同一性的序列表示，例如与SEQ ID NO:717或718具有至少50％、51％、52％、53％、54％、55％、56％、57％、58％、59％、60％、61％、62％、63％、64％、65％、66％、67％、68％、69％、70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或100％的序列同一性。SEQ ID NO:717是融合至MccE的Cvi的核酸分子。SEQ ID NO:718是融合至MccE的C末端部分的Cvi的核酸分子，如本文先前所述。
核心序列的此类同一性变体可用于本文实施方式的方法、用途、组合物、宿主和核酸，只要它们至少表现出如本文先前所述衍生它们的分子的实质活性即可。在一些本文实施方式的方法、用途、组合物、宿主和核酸中，其产物赋予对单种或对多于一种或对至少两种细菌素的免疫力的这些本文所述核酸分子的每一种可以可操作地连接至如本文所述的启动子。在一些实施方式中，所述启动子是弱启动子。在一些实施方式中，所述弱启动子是由SEQ ID NO:708表示的proC启动子或由SEQ ID NO:707表示的P24启动子，这已被实验证实(参见例如实施例3)。
适合用在本文实施方式的方法、用途、组合物、宿主和核酸中的构建体可以包含第一核酸分子，该第一核酸分子的产物赋予对细菌素的免疫力，并且这些构建体可以包含SEQID NO:702、703、710、711、704、705、712、713或714，基本上由其组成或者由其组成。这些构建体中的每一个已在本申请的实验部分中进行了广泛描述，其表明，这些构建体中的每一个均已被实际构建并且被证实根据本文一些实施方式是合适的(参见例如实施例1和2和3)。
在一些实施方式的方法中，添加到培养基中的细菌素是如本文所述的B17和/或C7和/或ColV。
所述方法可以允许生产任何目标产物。在一些实施方式的方法中，目标产物是微生物生物质、自复制染色体外核酸分子、所述第二核酸序列的转录物、由所述第二序列编码的多肽或者由所述多肽直接或间接产生的代谢物。
在一些实施方式的方法中，在培养步骤c)结束时对目标产物进行纯化。这可以使用技术人员已知的技术来进行。由于与自复制染色体外核酸分子的存在相关的能量负担已被最小化，预期目标产物的产量是最佳的。
所述方法可以使用任何合适的微生物细胞，例如作为宿主。合适的微生物细胞列于说明书标题为“一般描述”的部分中。本身以及用于本文实施方式的方法、用途、组合物和宿主中的合适的微生物细胞包括但不限于：细菌(例如革兰氏阴性细菌，例如大肠杆菌)、酵母、丝状真菌或藻类。在一些实施方式中，在一些实施方式中，微生物细胞是合成微生物细胞。
在一方法中，自复制染色体外核酸分子上存在的第一核酸序列可以可操作地连接到启动子。在一些实施方式中，所述启动子是弱启动子。在一些实施方式中，所述启动子是组成型启动子。在一些实施方式中，所述启动子是诱导型的。在一些实施方式中，所述启动子是弱组成型启动子。在一些实施方式中，在一些实施方式中，所述启动子是弱诱导型启动子。所述启动子的可诱导性是控制第一核酸序列的遗传活性的存在的一种方式。启动子是本领域众所周知的。在说明书的专用于一般描述的部分中提供了详细描述。启动子可用于驱动一个或多个编码序列的转录。可选地，自复制染色体外核酸分子包含参与生产目标产物的第二核酸序列，其中所述第二核酸序列的遗传活性独立于第一序列的遗传活性而被控制。
在一个实施方式中，所述第二核酸序列的遗传活性的控制并不独立于所述第一序列的遗传活性的控制。
在一些实施方式中，第二启动子驱动所述第二核酸序列的表达，所述第二核酸序列参与本文所述目标产物的生产。在一个实施方式中，第一启动子驱动如本文所述的免疫调节剂多核苷酸的表达。
可在本文中使用的启动子对于与其可操作连接的核酸分子可能不是天然的，即启动子对于与其可操作连接的核酸分子(编码序列)是异源的。尽管一些实施方式的启动子对于与其可操作地连接的编码序列是异源的，但是一些些实施方式中，启动子与微生物细胞是同源的，例如内源性的。在一些实施方式中，与天然于编码序列的启动子相比，异源启动子(对于核苷酸序列)能够产生包含更高稳态水平的编码序列的转录物(或能够每单位时间产生更多的转录物分子，即mRNA分子)。一些启动子可以随时驱动转录(“组成型启动子”)。一些启动子仅在选定情况下驱动转录(|“条件启动子”或“诱导型启动子”)，例如取决于环境条件的存在与否、化合物、基因产物、细胞周期的阶段等。
本领域技术人员可以理解的是，取决于所需的表达活性，可以选择合适的启动子，并且与待表达的序列顺式放置(即或与待表达的序列可操作连接)。本文表3.1-3.11提供了具有示例性活性的示例性启动子。本领域技术人员可以理解的是，一些启动子与特定的转录机制相容(例如RNA聚合酶、一般转录因子等)。如此，虽然鉴定了一些本文所述启动子的相容“物种”，但可以预期的是，根据本文的一些实施方式，这些启动子可以容易地在除已鉴定物种之外的微生物中起作用，例如在具有相容内源转录机制的物种、包含相容转录机制的转基因物种或包含相容转录机制的全合成微生物中。
本文表3.1-3.11的启动子可从Biobricks基金会公开获得。值得注意的是，Biobricks基金会鼓励根据BioBrickTM公共协议(BPA)使用这些启动子。
应当理解的是，本文所述的任何“编码”多核苷酸(例如参与目标产物生产的第一核酸序列和/或第二核酸序列)通常适于在所需启动子的控制下表达。在一个实施方式中，第一核酸序列受第一启动子的控制。在一个实施方式中，参与目标产物生产的第二核酸序列受第二启动子的控制。
通常，特定转录物的翻译起始受转录物编码序列5’端的特定序列调控。例如，编码序列可以以配置为与启动子tRNA配对的起始密码子开始。虽然天然存在的翻译系统通常使用Met(AUG)作为起始密码子，但容易理解的是，可以设计启动子tRNA使其与任何所需的三联体或多个三联体结合，并由此，除了AUG之外的三联体在某些实施方式中也可以作为起始密码子起作用。此外，起始密码子附近的序列可以促进核糖体组装，例如典型真核翻译系统中的Kozak序列((gcc)gccNccAUGG,SEQ ID NO:542，其中N代表“A”或“G”)或内部核糖体进入位点(IRES)、或典型原核翻译系统中的Shine-Dalgarno序列(GGAGGU,SEQ ID NO:543)。因此，在一些实施方式中，包含“编码”多核苷酸序列(例如第一核酸序列或参与发醇产物生产的第二核酸序列)的转录物包含适当的起始密码子和翻译起始序列。在一些实施方式中，例如，如果两个或更多个“编码”多核苷酸序列位于转录物上的顺式位置，则每个多核苷酸序列均包含适当的起始密码子和翻译起始序列。
在一些实施方式中，例如，如果两个或更多个“编码”多核苷酸序列位于转录物上的顺式位置，则这两个序列受单个翻译起始序列的控制，并且提供可以同时与两个以顺式编码的多肽一起起作用的单个多肽，或者提供用于分离两个以顺式编码的多肽的装置，例如2A序列等。在一些实施方式中，翻译起始物tRNA是可调节的，从而调节免疫调节剂或工业上有用分子的翻译起始。
表3.1：示例性的金属敏感性启动子
SEQ ID NO:
名称
描述
544
BBa_I721001
前导启动子
545
BBa_I731004
FecA启动子
546
BBa_I760005
Cu-敏感性启动子
547
BBa_I765000
Fe启动子
548
BBa_I765007
Fe和UV启动子
549
BBa_J3902
PrFe(PI+PII rus操纵子)
表3.2：示例性的细胞信号响应启动子
表3.3：示例性组成型大肠杆菌σ70启动子
表3.4：示例性组成型大肠杆菌σ8启动子
SEQ ID NO:
名称
描述
654
BBa_J45992
全长固定相osmY启动子
655
BBa_J45993
最小固定相osmY启动子
表3.5：示例性组成型大肠杆菌σ32启动子
SEQ ID NO:
名称
描述
656
BBa_J45504
htpG热休克启动子
表3.6：示例性组成型枯草芽孢杆菌σA启动子
SEQ ID NO:
名称
描述
657
BBa_K143012
启动子veg，用于枯草芽孢杆菌的组成型启动子
658
BBa_K143013
启动子43，用于枯草芽孢杆菌的组成型启动子
659
BBa_K780003
用于枯草芽孢杆菌的强组成型启动子
660
BBa_K823000
PliaG
661
BBa_K823002
PlepA
662
BBa_K823003
Pveg
表3.7：示例性组成型枯草芽孢杆菌σB启动子
SEQ ID NO:
名称
描述
663
BBa_K143010
用于枯草芽孢杆菌的启动子ctc
664
BBa_K143011
用于枯草芽孢杆菌的启动子gsiB
665
BBa_K143013
启动子43，用于枯草芽孢杆菌的组成型启动子
表3.8：来自杂原核生物的示例性组成型启动子
表3.9：来自噬菌体T7的示例性组成型启动子
SEQ ID NO:
名称
描述
668
BBa_I712074
T7启动子(来自T7噬菌体的强启动子)
669
BBa_I719005
T7启动子
670
BBa_J34814
T7启动子
671
BBa_J64997
T7共有-10及其余
672
BBa_K113010
重叠T7启动子
673
BBa_K113011
更多重叠T7启动子
674
BBa_K113012
减弱重叠T7启动子
675
BBa_R0085
T7共有启动子序列
676
BBa_R0180
T7 RNAP启动子
677
BBa_R0181
T7 RNAP启动子
678
BBa_R0182
T7 RNAP启动子
679
BBa_R0183
T7 RNAP启动子
680
BBa_Z0251
T7强启动子
681
BBa_Z0252
T7弱结合和进行性
682
BBa_Z0253
T7弱结合启动子
表3.10：来自酵母的示例性组成型启动子
表3.11：来自杂真核生物的示例性组成型启动子
SEQ ID NO:
名称
描述
697
BBa_I712004
CMV启动子
698
BBa_K076017
Ubc启动子
仅以非限制性示例的方式提供上述启动子。启动子可以是合成的启动子。已经在本文先前描述了适合用于本文一些实施方式的方法、用途、组合物、宿主和核酸的启动子，例如已经根据本文一些实施方式实验证实的由SEQ ID NO:708表示的proC(参见实施例2)和已经根据本文一些实施方式实验证实的由SEQ ID NO:707表示的P24(参见实施例3)。在一些实施方式中，适合的诱导型启动子是P24 LacO杂交启动子，其在LacI存在下会被抑制且在IPTG存在下会具有活性。该启动子已经根据本文一些实施方式进行了实验证实(参见实施例3)。
本领域技术人员可以容易地认识到，上述启动子的许多变体以及许多其他启动子(包括从天然存在的生物体分离出来的启动子、其变体以及完全合成或工程化的启动子)可以根据本文一些实施方式容易地使用。当在与编码转录物的核酸分子可操作连接的对照或参照质粒中进行测试时，当所述质粒存在于细胞中时，存在可检测量的所述转录物分子，则可以说变体、全合成或合成或工程启动子是具有活性的或功能性的，并由此可以用于本文实施方式的方法、用途、组合物、宿主和核酸中。变体、全合成或合成或工程启动子可以具有衍生它的启动子的活性的至少50％、至少60％、至少70％、至少80％、至少90％或至少100％。
可选地，所述方法包含在允许已对接收自复制染色体外核酸分子的宿主存活的条件下使用自复制染色体外核酸分子转化所述微生物宿主。值得注意的是，在一些实施方式中，可以在微生物细胞中提供自复制染色体外核酸分子(例如，如果微生物细胞或其前身已被自复制染色体外核酸分子转化)，并由此在一些实施方式中，在所述方法中不需要转化步骤。可以在步骤c)的培养之前进行转化步骤。在一些实施方式中，在步骤a)之前提供转化步骤，由此提供包含自复制染色体外核酸分子的宿主细胞。在一些实施方式中，在所述转化步骤中使用的自复制染色体外核酸分子过时一步包含可选步骤b)的第二核酸。
基因改造微生物的技术是本领域众所周知的(例如参见Molecular CloningFourth edition,2012Cold Spring Harbor Laboratory Press,A laboratory manual,由M.R.Green和J Sambrook著,其通过引用以其全部内容并入本文)。在一些实施方式中，微生物被基因改造以包含自复制染色体外核酸分子，自复制染色体外核酸分子包含第一核酸序列和可选的参与生产目标产物的分子。可以将多核苷酸或核酸分子递送至微生物。
在一个实施方式中，通过对应于允许已经接收自复制染色体外核酸分子的细胞存活的至少一个给定条件的所述第一核酸序列的遗传活性来正向选择微生物细胞，并且所述条件可以是环境条件。环境条件可以是培养基。
灵活地基因改造微生物细胞可能是有用的，例如工程化或再工程化微生物细胞以具有所需类型和/或谱的遗传活性。在一些实施方式中，提供了一种用于***一个或多个所需独特的第一核酸序列的盒。示例性的盒包括但不限于Cre/lox盒或FLP/FRT盒。
在一个实施方式中，微生物细胞包含多于一种(多于两种、多于三种…)包含如本文所述的第一核酸序列的不同自复制染色体外核酸分子，意味着所述细胞可以表现出多于一种(多于两种、多于三种…)遗传活性，每种遗传活性向所述细胞赋予优势。如果在每种所述不同的自复制染色体外核酸分子存在第一启动子，则每种所述第一启动子可以不同或相同。因此，在用于生产目标产物的方法中使用一种、两种、三种、四或更多种不同的细菌素也在本文实施方式的方法、用途、组合物、宿主和核酸的范围内，其中微生物宿主包含一种、两种、三种、四种或更多各路不同的染色体外核酸分子，每种向所述微生物宿主赋予不同的遗传活性。替代地，使用其产物提供对至少不同细菌素的免疫力的单个核酸分子也在本文实施方式的方法、用途、组合物、宿主和核酸的范围内。本文已经描述了此类核酸分子。
在一些实施方式中，可以使用质粒接合以将所需的质粒从|“供体”微生物细胞引入到受体微生物细胞(Goni-Moreno,et al.(2013)Multicellular Computing UsingConjugation for Wiring.PLoS ONE 8(6):e65986,在此通过引用以其全部内容并入)。在一些实施方式中，质粒接合可以通过将来自供体微生物细胞的接合质粒引入到受体微生物细胞来基因改造受体微生物细胞。不受任何特定理论的限制，包含相同或功能相同的复制基因集的接合质粒通常不能在同一微生物细胞中共存。因此，在一些实施方式中，质粒接合通过引入新的接合质粒来代替受体细胞中存在的另一接合质粒，从而“重编程”受体微生物细胞。在一些实施方式中，使用质粒接合来以第一核酸分子(其在一些实施方式中可以编码免疫调节剂)的特定组合来工程化(或再工程化)同生物细胞。根据一些实施方式，提供了包含第一酸序列(其在一些实施方式中可以编码免疫调节剂)的不同组合的各种接合质粒。所述质粒可包含本文所述的其他遗传元件，例如启动子、翻译起始位点等。在一些实施方式中，在供体细胞的集合中提供所述多种接合质粒，以便可以选择包含所需质粒的供体细胞进行质粒接合。在一些实施方式中，选择了免疫调节剂的特定组合，并且将适合的供体细胞与目标微生物细胞接合以将包含所述组合的接合质粒引入到受体细胞。在一些实施方式中，受体细胞是“新工程化的”细胞，例如待引入或用于起始培养。
步骤b)
除了步骤a)之外，在一些实施方式中，所述方法进一步包含可选的步骤b)，其中自复制染色体外核酸分子包含参与所述目标产物生产的第二核酸序列，其中所述第二核酸序列的遗传活性独立于所述第一序列的遗传活性而是受控的。
在本文实施方式的方法、用途、组合物、宿主和核酸的背景下，表达“独立地控制”具有本领域技术人员在考虑到本公开后所理解的其惯常和普通含义，包括是指使用不同的方式来控制所述第一和第二核酸序列的遗传活性。本文已经详细描述了控制核酸序列遗传活性的方式。
步骤c)
在一些实施方式中，步骤a)(其可选地包括如本文所述的转化)和可选的步骤b)之后是步骤c)，其包含在允许所述转化的微生物宿主将所述第一核酸序列表达至给定水平以维持自所述复制染色体外分子进入不断生长的微生物种群的条件下，培养所述转化的微生物宿主。在一些实施方式中，可选地控制编码所述目标产物的第二序列。
在一些实施方式的方法中，步骤c)条件的至少一部分使得所述第一核酸序列不展示出所述遗传活性。“步骤c)的一部分”是指例如步骤c)持续时间的至少20％、30％、40％、50％、60％、70％、80％或90％或多达100％。所述方法的这一实施方式是相当有吸引力的，因为步骤c)的一部分在不存在第一核酸序列的遗传活性的条件下进行。所述遗传活性的存在构成对微生物宿主细胞的能量负担，并且并非始终需要以保持目标产物的合适生产水平。在一些实施方式中，设想了使步骤c)的一部分不具有第一核酸序列的遗传活性，之后是具有所述活性的部分。在步骤c)期间，这两部分可以重复一次或更多次。
可以在任何合适的微生物培养环境中培养微生物细胞。微生物培养环境可以包含多种培养基，例如原料(feedstock)。特定培养基的选择可以取决于所需的应用。培养基的条件不仅包括化学组成，还包括温度、光量、pH、CO2水平等。培养基可以包含细菌素。在一个实施方式中，诱导细菌素活性的化合物存在于原料外部，而不存在于原料中。
在一个实施方式中，将本文所述的基因工程或转化的微生物添加到包含至少一种原料的培养基中。在一个实施方式中，培养基包含诱导免疫调节剂活性或表达的化合物。
术语“原料”具有本领域技术人员在考虑本公开后所理解的其惯常和普通含义，并且涵盖例如在工艺生产过程中可以微生物消耗、发酵、纯化、修饰或以其他方式处理的材料。因此，“原料”不限于食物或食品。如本文所用，“原料”是一类培养基。因此，如本文所用，“培养基”包括但不限于原料。因此，每当在本文中提及“培养基”时，也明确考虑了原料。
在培养转化的微生物细胞之前，可能有用的是确定效果(如果有)或优选允许已经接收自复制染色体外核酸分子的宿主存活并可选地生长的条件。
在一些实施方式中，提供了包含自复制染色体外核酸分子的微生物细胞或微生物宿主或微生物宿主细胞或合成微生物宿主细胞，其包含遗传活性向微生物宿主赋予优势的第一核酸序列并且可选地包含直接或间接参与目标产物生产的第二核酸序列，其中所述第一核酸序列的遗传活性是受控的。
在一些实施方式中，提供了一种自复制染色体外核酸分子，其包含遗传活性向微生物宿主赋予优势的第一核酸序列并且可选地包含直接或间接参与目标产物生产的第二核酸序列，其中所述第一核酸序列的遗传活性是受控的。
本文已经描述了该微生物宿主和该自复制染色体外核酸分子的各特征。
一般描述
本文使用的术语具有本领域技术人员在阅读本公开时所理解的其惯常和普通含义，并且可以包括以下一般描述。
微生物
如本文所用，“微生物(microbial organism)”、“微生物(microorganism)”、“微生物细胞”或“微生物宿主”及这些根术语的变型(如复数形式等)具有本领域技术人员在考虑到本公开后所理解的其惯常和普通含义，包括任何天然存在的物种或合成的或完全合成的原核或真核单细胞生物体以及古细菌种。因此，此表达可以指细菌种、真菌种和藻类的细胞。可以根据本文实施方式使用的示例性微生物包括但不限于细菌、酵母、丝状真菌和藻类，例如光合微藻。另外，可以合成完全合成的微生物基因组并将其移植到单个微生物细胞中，以产生能够连续自我复制的合成微生物(参见Gibson et al.(2010),"Creation of aBacterial Cell Controlled by a Chemically Synthesized Genome,"Science 329:52-56，在此通过引用以其全部内容并入)。因此，在一些实施方式中，微生物是全合成的。可以在所需的底盘上将遗传元件的所需组合，包括调控基因表达的元件、和编码基因产物(例如免疫调节剂、毒剂、解毒剂和工业上有用的分子，也称为目标产物)的元件，组装成部分或全合成的微生物。在Wright,et al.(2013)"Building-in biosafety for syntheticbiology"Microbiology 159:1221-1235中也可以找到用于工业应用的基因工程微生物的描述。遗传元件的合适实施方式将在本文后面描述。
根据本文的实施方式，可以使用多种细菌物种和菌株，并且可以提供基于已知种类的“底盘(chassis)”的基因改造的变体或合成细菌。可以根据本文实施方式使用的具有工业适用特性的示例性细菌包括但不限于芽孢杆菌属种(例如凝结芽孢杆菌、枯草芽孢杆菌和地衣芽孢杆菌)、类芽孢杆菌种、链霉菌种、微球菌种、棒状杆菌种、醋酸杆菌种、蓝细菌种、沙门氏菌种、红球菌种、假单胞菌种、乳酸杆菌种、肠球菌种、产碱菌种、克雷伯菌种、类芽孢杆菌物种、关节杆菌种、棒状杆菌物种、短杆菌种、水生栖热菌、施氏假单胞菌、热纤梭菌和大肠杆菌。
根据本文的实施方式，可以使用多种酵母物种和菌株，并且可以提供基于已知物种的“底盘”的基团改造的变体或合成酵母。可以根据本文实施方式使用具有工业适用特性的示例性酵母包括但不限于酵母菌种(例如酿酒酵母、贝酵母、布拉氏酵母)、念珠菌种(例如产朊假丝酵母、克柔假丝酵母)、裂殖酵母菌种(例如粟酒裂殖酵母、日本裂殖酵母)、毕赤酵母或汉逊酵母种(例如巴斯德毕赤酵母或多形汉逊酵母)和酒香菌属(例如克劳森酒香酵母)。
根据本文实施方式可以使用各种藻类物种和菌株，并且可以创建基于已知物种“底盘”的基因改造的变体或合成藻类。在一些实施方式或，藻类包括光合微藻，基本上由其组成或由其组成。可以用于生物燃料并且可以根据本文实施方式使用的示例性藻类物种包括布朗葡萄藻、小球藻种、杜氏盐藻、江蓠物种、颗石藻(Pleurochrysis carterae)和海藻物种。此外，许多藻类可用于食品、化肥产品、废物中和、环境修复和碳水化合物生产(例如生物燃料)。
根据本文的实施方式可以使用多种丝状真菌物种和菌株，并且可以提供基于已知物种“底盘”的基因改造变体或合成丝状真菌。可以根据本文的实施方式使用的具有工业适用特性的示例性丝状真菌包括但不限于枝顶孢属、伞菌属、链格孢属、曲霉属、短梗霉、葡萄球菌、拟蜡菌属、Chaetomidium、金孢属、麦角菌属、旋孢腔菌、鸡腿菇、家白蚁(Coptotermes)、棒囊壳属、丛赤壳属、隐球菌属、色二孢属、黑耳属、Filibasidium、镰胞菌、赤霉菌属、全鞭毛虫属、腐质霉属、lrpex、香菇、Leptospaeria、稻瘟病菌、黑果蛋白菌(Melanocarpus)、亚灰树花菌(Meripilus)、毛霉菌、毁丝霉属、Neocaffimastix、脉孢菌、拟青霉属、青霉菌、平革菌属、瘤胃壶菌属、Poitrasia、假黑盘菌属、假披发虫属(Pseudotrichonympha)、根毛霉、裂褶菌属、柱顶孢霉、踝节菌属、嗜热子囊菌属、梭孢壳属、弯颈霉属、木霉属、长毛盘菌属、轮枝孢属、小包脚菇属或炭角菌属。
物种包括纤维素枝顶孢霉、棘孢曲霉、泡盛曲霉、佛曲霉、烟曲霉、日本曲霉、构巢曲霉、黑曲霉、米曲霉、Chrysosporium inops、嗜角质金孢子菌、卢克诺文思金孢子菌(Chrysosporium lucknowense)、粪状金孢子菌(Chrysosporium merdarium)、潘氏金孢菌(Chrysosporium pannicola)、昆士兰金孢菌(Chrysosporium queenslandicum)、热带金孢子菌、带状金孢菌(Chrysosporium zona turn)、孢状镰孢、枯萎镰孢菌、Fusariumcrookwellense、黄色镰孢菌、禾谷镰孢菌、禾赤镰孢、异孢镰孢、合欢木镰孢、尖孢镰孢、多枝镰孢、粉红镰孢、接骨木镰孢、肤色镰孢、拟枝孢镰孢、硫色镰孢、圆镰孢(Fusariumtorulosum)、拟丝孢镰孢、镶片镜抱菌(Fusarium venenaturn)、灰腐质霉、特异腐质霉、柔毛腐质霉、白耙齿菌、米黑毛霉、嗜热毁丝霉、粗糙脉孢菌、绳状青霉、产紫青霉、黄孢原毛平革菌、无色梭抱壳啊和(Thielavia achromatica)、成层梭抱壳菌(Thielavia albomyces)、白毛梭抱壳菌(Thielavia albopilosa)、澳洲梭抱壳菌(Thielavia australeinsis)、粪梭抱壳菌(Thielavia fimeti)、小抱梭抱壳菌、卵抱梭抱壳菌(Thielavia ovispora)、秘鲁梭抱壳菌(Thielavia peruviana)、毛梭抱壳菌(Thielavia setosa)、瘤抱梭抱壳菌(Thielavia spededonium)、耐热梭抱壳菌(Thielavia subthermophila)、上生梭抱壳菌(Thielavia terrestris)、哈茨木霉、康氏木霉、长枝木霉、里氏木霉或绿色木霉。
抗生素
“抗生素”及该根术语的变型具有本领域技术人员考虑本公开后所理解的其惯常和普通含义，包括可以杀死或阻止至少一个微生物细胞生长代谢物或代谢途径的中间体。一些抗生素可以通过微生物细胞(例如细菌)来生产。一些抗生素可以化学合成。应当理解的是，细菌素与抗生素不同，至少因为细菌素是指基因产物(其在一些实施方式中经历额外的翻译后加工)或其合成类似物，而抗生素是指代谢途径的中间体或产物或它们的合成类似物。
序列同一性和相似性
序列同一性具有本领域技术人员考虑本公开后所理解的其惯常和普通含义，包括通过比较序列确定的两个或多个氨基酸(多肽或蛋白质)序列或两个或多个核酸(多核苷酸)序列之间的关系。通常，在所比较的序列的整个长度上对比序列同一性或相似性。在本领域，“同一性”还可以指氨基酸或核酸序列之间视情况而定的序列相关性程度，其由此类序列的串之间的匹配确定。可以通过将一个多肽的氨基酸序列及其保守氨基酸取代物与第二个多肽的序列进行比较来确定两个氨基酸序列之间的“相似性”。可以通过本领域技术人员已知的各种方法容易地计算“同一性”和“相似性”。在一些实施方式中，通过比较本文鉴定的序列的全长来确定序列同一性。
一些确定同一性的方法被设计为在测试的序列之间提供最大的匹配。确定同一性和相似性的方法已经编纂在公众可获得的计算机程序中。确定两个序列之间的同一性和相似性的计算机程序方法包括例如BestFit、BLASTP、BLASTN和FASTA(Altschul,S.F.et al.,J.Mol.Biol.215:403-410(1990)，公众可从NCBI及其他来源获得(BLAST Manual,Altschul,S.,et al.,NCBI NLM NIH Bethesda,MD 20894)。使用的算法可以是EMBOSS(可从以下网址访问万维网：www.ebi.ac.uk/emboss/align)。使用EMBOSS进行氨基酸序列比较的参数可以包括缺口打开10.0，缺口延伸0.5，Blosum 62矩阵。使用EMBOSS进行核酸序列比较的参数可以包括缺口打开10.0、缺口延伸0.5，DNA全矩阵(DNA同一性矩阵)。
可选地，在确定氨基酸相似性程度时，本领域技术人员还可以考虑所谓的“保守”氨基酸取代，正如本领域技术人员将清楚的那样。保守氨基酸取代是指具有相似侧链的残基的互换性。例如，具有脂肪族侧链的一组氨基酸是甘氨酸、丙氨酸、缬氨酸、亮氨酸和异亮氨酸；具有脂肪族羟基侧链的一组氨基酸是丝氨酸和苏氨酸；具有含酰胺侧链的一组氨基酸是天冬酰胺和谷氨酰胺；具有芳香族侧链的一组氨基酸是苯丙氨酸、酪氨酸和色氨酸；具有碱性侧链的一组氨基酸是赖氨酸、精氨酸和组氨酸；且具有含硫侧链的一组氨基酸是半胱氨酸和蛋氨酸。合适的保守氨基酸取代组包括：缬氨酸-亮氨酸-异亮氨酸，苯丙氨酸-酪氨酸，赖氨酸-精氨酸，丙氨酸-缬氨酸，和天冬酰胺-谷氨酰胺。本文公开的氨基酸序列的取代变体是其中公开序列中的至少一个残基已被去除并且在其位置***了不同残基的那些变体。在一些实施方式中，氨基酸变化是保守的。每种天然存在的氨基酸的合适保守取代包括：Ala至ser；Arg至lys；Asn至gln或his；Asp至glu；Cys至ser或ala；Gln至asn；Glu至asp；Gly至pro；His至asn或gln；Ile至leu或val；Leu至ile或val；Lys至arg；gln或glu；Met至leu或ile；Phe至met,leu或tyr；Ser至thr；Thr至ser；Trp至tyr；Tyr至trp或phe；以及，Val至ile或leu。
同源
术语“同源”具有本领域技术人员在考虑本公开后所理解的其惯常和普通含义，包括当用于指示给定(重组)核酸或多肽分子与给定宿主生物体或宿主细胞之间的关系时，可以理解为是指在自然界中，核酸或多肽分子是由相同物种的宿主细胞或生物体(可选相同品种或菌株)产生的。如果与宿主细胞同源，则编码多肽的核酸序列通常会与其天然环境中不同的另一启动子序列可操作地连接。当用于指示两个核酸序列的相关性时，术语“同源”具有本领域技术人员在考虑本公开后所理解的其惯常和普通含义，并且可以是指一种可与互补的单链核酸序列杂交的单链核酸序列。杂交程度可能取决于许多因素，包括序列之间的同一性数量和杂交条件，例如先前提及的温度和盐浓度。同一性区域可以大于约5bp，同一性区域可以大于10bp。在一些实施方式中，当两个核酸或多肽序列具有大于80％的同一时，则可以说它们是同源的。
异源
术语“异源”具有本领域技术人员在考虑本公开后所理解的其惯常和普通含义，包括当用于核酸(DNA或RNA)或蛋白质时，其可以是指一种核酸或蛋白质(也称为多肽或酶)，该核酸或蛋白质不天然地作为其所存在的生物体、细胞、基因组或DNA或RNA序列的一部分而存在，或者与在自然界发现所述核酸或蛋白质的细胞或者基因组或DNA或RNA序列中的位置或多个位置相比，该核酸或蛋白质是在不同的细胞或者DNA或RNA序列中的位置或多个位置发现的。异源核酸或蛋白质对于引入它的细胞而言不是内源的，而是从另一细胞获得的或合成或重组产生的。通常，尽管不是必然，这种核酸编码的蛋白质通常不是转录或表达所述DNA的细胞通常产生的蛋白质。类似地，外源RNA编码在存在该外源RNA的细胞中通常不表达的蛋白质。异源核酸和蛋白质也可以称为外来核酸或蛋白质。在本文中，术语“异源核酸或蛋白质”涵盖会被本领域技术人员公认为与表达它的细胞异源或外来的任何核酸或蛋白质。术语异源也适用于核酸或氨基酸序列的非天然组合，即其中组合序列中的至少两个相对于彼此是外来的组合。
可操作地连接
如本文所用，术语“可操作地连接”具有本领域技术人员在考虑本公开后所理解的其惯常和普通含义，并且可以是指功能关系中多核苷酸元件(或编码序列或核酸序列或核酸分子)的连接。当核酸序列与另一核酸序列处于功能关系时，该核酸序列是“可操作连接的”。例如，如果启动子或增强子影响编码序列的转录，则该启动子或增强子可操作地与该编码序列连接。可操作地连接是指被连接的核酸序列通常是连续的，并且在需要连接两个蛋白质编码区的情况下，是连续的并且在阅读框中。
启动子
如本文所用，术语“启动子”具有本领域技术人员在考虑本公开后所理解的其惯常和普通含义，并且可以是指核酸片段，其用于控制一个或多个核酸分子的转录，相对于核酸分子的转录起始位点的转录方向位于上游，并且通过DNA依赖性RNA聚合酶结合位点、转录起始位点和任何其他DNA序列(包括但不限于转录因子结合位点、阻遏物和激活蛋白结合位点、以及本领域技术人员已知直接或间接调节/控制从启动子的转录量的任何其他核苷酸序列)的存在在结构上进行鉴定。“组成型”启动子是在大多数环境和发育条件下都具有活性的启动子。“诱导型”启动子是在环境或发育调节下具有活性的启动子。
在本文件及其权利要求中，动词“包含”及其词形变化以非限制性意义使用，是指包括该词语后的项目，但不排除未具体提及的项目。此外，可以由“基本上由…组成”替换的动词“由…组成”是指如本文限定的自复制染色体外核酸分子、微生物宿主(或方法)除了明确指定的组成(或步骤)之外还可以包含附加组分(或附加步骤)，所述附加组分(或附加步骤)不改变本发明的独特特性。另外，此外，通过不定冠词“一个(a)”或“一种(an)”提及的要素不排除存在多于一个所述要素的可能性，除非上下文明确要求有且只有一个所述要素。因此，不定冠词“一个(a)”或“一种(an)”通常是指“至少一个”。
本说明书中引用的所有专利和参考文献均通过引用全文并入本文。以下实施例仅提供用于说明性目的，并不旨在以任何方式限制本发明的范围。
序列表(DNA，除非另有说明)
SEQ ID NO:1粪肠球菌肽
SEQ ID NO:2细菌素的特征性基序
SEQ ID NO:3杂合细菌素，Ent35-MccV
SEQ ID NO:4-698,720-759：表1-3中的序列(半DNA/半蛋白质，如表中所示)
SEQ ID NO:699McbG
SEQ ID NO:700MccE
SEQ ID NO:701MccE的C末端部分
SEQ ID NO:702pSyn2-McbG
SEQ ID NO:703pSyn2-McbE/F
SEQ ID NO:704pMcbG1.0
SEQ ID NO:705pMcbG.1.1.
SEQ ID NO:706MccE的C末端部分(氨基酸)
SEQ ID NO:707P24启动子
SEQ ID NO:708proC启动子
SEQ ID NO:709Cvi
SEQ ID NO:710proC-McbG-CterMccE(proc)(Cter可以被C-末端替代)
SEQ ID NO:711proC-Cvi-Cter-MccE(proc)(Cter可以被C-末端替代)
SEQ ID NO:712pBACT5.0
SEQ ID NO:713pBACT2.0
SEQ ID NO:714pBACT5.0-mcherry
SEQ ID NO:715McbG-MccE
SEQ ID NO:716McbG-Cter部分MccE(Cter可以被C-末端替代)
SEQ ID NO:717Cvi-MccE
SEQ ID NO:718Cvi-Cter部分MccE(Cter可以被C-末端替代)
SEQ ID NO:719:载体pUC-ColV
附图说明
图1：构建体：pSyn2-McbE/F：在Ptac下含有基因McbE和F。
图2：构建体：pSyn2-McbG：在Ptac下含有基因McbG。
图3：构建体：pMcbG 1.1：在P24含有基因McbG。
图4：构建体：pMcbG 1.0：在P24 LacO下含有基因McbG。
图5：pBACT5.0载体
图6：pBACT2.0载体
图7：pBACT5.0-mcherry载体
图8：调谐启动子。在不存在感应子(上部分)的情况下，阻遏物(repressor)可以结合操纵子并阻止选择基因的表达。在存在感应子(下部分)的情况下，阻遏物不与允许选择基因表达的操纵子结合。
图9：在具有KanR(pKan-pLac)和具有针对小菌素C7和ColV的2个免疫力(pBACT6.0-pLac)的大肠杆菌中蛋白质X过表达的比较。在SDS-PAGE中分析了5mg总提取物。
图10：在具有KanR(pKan-T7prom)和具有针对小菌素C7和ColV的2个免疫力(pBACT5.0-T7prom)的大肠杆菌中iota-卡拉胶酶蛋白质过表达的比较。在SDS-PAGE中分析了5mg总提取物。
图11：在具有KanR(pKan-T7prom)和具有针对小菌素C7和ColV的2个免疫力(pBACT5.0-T7prom)的大肠杆菌中λ-卡拉胶酶蛋白质过表达的比较。在SDS-PAGE中分析了5mg总提取物。
具体实施方式
实施例
实施例1：细菌素B17和C7作为选择剂的用途
1.细菌素B17、C7和ColV的生产
使用的菌株：C600：F-tonA21 thi-1thr-1 leuB6 lacY1 glnV44 rfbC1 fhuA1λ-
描述于Appleyard Genetics 39(1954),440-452中。
下表中描述了用于生产Mic B17的载体。
表X：用于生产Mic B17的载体
这些构建体详细地描述于Rodriguez-Sainz,M.C.,C.etal.1990.Mol.Microbiol.4:1921-1932中。
用于生产Mic C7的载体是Pp70。该载体基于pBR322并且携带有～6000bp具有mcc基因簇的DNA片段(如Zukher I et al,Nucleic Acids Research,2014,Vol.42,No.1911891–11902中所述)。
用于生产ColV的载体是pUC-ColV(SEQ ID NO:719)。该载体基于pUC57并且携带有～5000bp具有ColV基因簇的DNA片段。
使包藏这些重组载体的菌株在37℃下在LB培养基中生长。过夜培养后，离心发酵后的培养基并且上清液在0.2微米滤膜上呈红色。
通过含有敏感菌株的平板上的扩散抑制生长的大小来估算上清液中存在的细菌素活性。
2.结果
我们证明了我们可以使用表现出B17、C7或ColV活性的上清液作为经典抗生素(诸如Amp、Kan或Chlo)添加到培养基中。将呈现此种细菌素活性的上清液在-20℃存储数月(至少12个月)，我们没有观察到活性的显著降低。将含有具有此类细菌素活性的培养基的培养皿在+4℃下存诸数周(至少4周)，我们没有观察到活性降低。因此，我们证明了培养基中存在的此类B17、C7或ColV活性是稳定的。
3.结论
在实验室发酵产生的细菌素B17、C7和ColV是易于生产、易于使用且在培养基中稳定的选择剂。这些性质类似于用作经典选择剂的抗生素的性质。
实施例2：鉴定赋予对C7和B17的抗性所必需的最小遗传元件
1.所需载体的构建
文献已经使得能够针对宿主自身细菌素的产生来确定产生宿主所需的元件，在B17细菌素:McbG用于B17，由SEQ ID NO:699表示和泵(McbE和McbF用于B17，由SEQ ID NO:703表示)的情况下也是如此。已知这些基因是必需的(或更准确地说，涉及防止细菌素B17的作用)。关于B17基因座的文献没有鉴定哪个是或是否足以提供抗性的元件。
我们从B17免疫结构中分离了基因，并将这些基因克隆到了诱导型启动子(Ptac)后面的载体中。
构建体：pSyn2-McbG(图2,SEQ ID NO:702)：在Ptac下含有基因McbG
构建体：pSyn2-McbE/F(图1,SEQ ID NO:703)：在Ptac下含有基因McbE和F
我们从B17免疫结构中分离了所述基因，并将它们克隆到了诱导型启动子(Ptac)后面的载体中。
我们已经证明，低McbG表达(Ptac未诱导)足以给出针对该菌株的抗性表型，另一方面，McbE/F的存在有毒并且不允许给出针对关于B17的存在而提供保护的响应。
2.结果
已令人惊讶地发现，由SEQ ID NO:701表示的MccE的C末端部分足以赋予针对细菌素C7的抗性。
我们已经证明，当克隆到载体中时，能够从质粒表达McbG和MccE基因(或截短的MccE，由SEQ ID NO:701表示)，以分别对B17和C7产生抗性，并且这些蛋白质可以在对这些小菌素/细菌素敏感的菌株中用作载体选择标志物。所使用的载体是pBACT2.0(图6,SEQ IDNO:713)。
SEQ ID NO:710表示构建体proC-McbG-CterMccE
我们已经证明，当克隆到载体中时，能够从质粒表达Cvi和MccE基因的C末端部分，以分别对ColV和C7产生抗性，并且这些蛋白质可以在对这些小菌素/细菌素敏感的菌株中用作载体选择标志物。所使用的载体是pBACT5.0(图5,SEQ ID NO:712)。
SEQ ID NO:711表示构建体proC-Cvi-CterMccE
3.结论
因此，可以使用由SEQ ID NO:701表示的小尺寸的单个节段作为针对C7的选择标志物。
实施例3：我们能否使用很少或不使用能量来从细菌由McbG生成选择性标志物？
1.构建的载体
为了回答这个问题，将McbG基因克隆在弱启动子P24(SEQ ID NO:707)下。Braatsch S et al,Biotechniques.2008Sep；45(3):335-7中描述了P24启动子。
我们***了B17 McbG免疫结构基因并且将后者克隆到了载体中，在弱组成型启动子(P24)的后面。我们使用P24 LacO杂交启动子生成了第二构建体，P24 LacO杂交启动子是在lacI存在下被抑制且在IPTG存在下具有活性的诱导型启动子。
构建体：pMcbG 1.0(图4,SEQ ID NO:704)，在P24 LacO下含有基因McbG。
构建体：pMcbG 1.1(图3,SEQ ID NO:705)，在P24下含有基因McbG。
使用的菌株如下：
BL21(DE3)：fhuA2[lon]ompT gal(λDE3)[dcm]ΔhsdS
λDE3＝λsBamHIoΔEcoRI-B int::(lacI::PlacUV5::T7基因1)i21Δnin5
使用载体pMcbG1.0或pMcbG1.1转化BL21(DE3)菌株(参见图3或4)。转化后，在含有B17的平板上选择转化株。重新生长分离的菌落，并用相关的限制性内切酶处理后，通过凝胶电泳分析它们包含的质粒。
2.结果
我们证明了，McbG的弱转录足以提供针对B17的抗性。此外，我们已经证明，这种选择标志物可通过P24 LacO启动子来诱导并且含有这种基因的载体仅在IPTG存在下给出抗性表型。
3.结论
可以将McbG基因组成性或诱导性地用作选择标志物。因此，已经证明，在过程中根据需要进行低水平的组成型表达以及诱导型表达，允许减少生产细胞的能量负担，而不会从生产细胞中损失质粒。
实施例4：m-cherry蛋白质的生产
SEQ ID NO:714表示用于生产m-cherry蛋白质的构建体。图7示出了这一构建体。
在IPTG存在下，随着培养皿上的细菌菌落变成红色，产生了m-cherry蛋白质并可视化。
实施例5：调谐启动子
我们制备了具有可调免疫力选择的载体(见图8)。调整启动子使我们可以将选择“打开”或“关闭”。这首次使得允许发酵过程中的选择性压力适应需求，例如根据宿主损失的重组质粒。通过限制宿主的能量负担，这可以提供优势。因此，此种载体改善了工业结果(重组产物)。另外，它们易于在任何菌株中使用(不需要宿主基因组中的特殊特征)且不需要抗生素。
实施例6：抗生素(卡那霉素)选择与免疫选择的比较
我们已将免疫选择应用于不同的重组蛋白。
图9显示了在具有KanR(pKan-pLac)和具有针对小菌素C7和ColV的2个免疫力(pBACT6.0-pLac)的大肠杆菌中蛋白质X过表达的比较。在SDS-PAGE中分析了5mg总提取物。
图10显示了在具有KanR(pKan-T7prom)和具有针对小菌素C7和ColV的2个免疫力(pBACT5.0-T7prom)的大肠杆菌中iota-卡拉胶酶蛋白质过表达的比较。在SDS-PAGE中分析了5mg总提取物。所使用的载体基于pBACT5.0载体(SEQ ID NO:712)。
图11显示了在具有KanR(pKan-T7prom)和具有针对小菌素C7和ColV的2个免疫力(pBACT5.0-T7prom)的大肠杆菌中λ-卡拉胶酶蛋白质过表达的比较。在SDS-PAGE中分析了5mg总提取物。所使用的载体基于pBACT5.0载体(SEQ ID NO:712)。
在本实施例中使用的弱组成型proC启动子允许减少宿主的能量负担。
序列表
<110> 辛格隆股份公司(Syngulon S.A.)
布鲁塞尔大学(Université libre de Bruxelles)
<120> 发酵工艺（Fermentation process）
<130> P6065030PCT
<150> EP17208600.1
<151> 19-12-2017
<160> 759
<170> PatentIn version 3.5
<210> 1
<211> 40
<212> PRT
<213> 粪肠球菌
<400> 1
Trp Leu Pro Pro Ala Gly Leu Leu Gly Arg Cys Gly Arg Trp Phe Arg
1 5 10 15
Pro Trp Leu Leu Trp Leu Gln Ser Gly Ala Gln Tyr Lys Trp Leu Gly
20 25 30
Asn Leu Phe Gly Leu Gly Pro Lys
35 40
<210> 2
<211> 5
<212> PRT
<213> 人工序列
<220>
<223> IIa类末端的N末端基序
<220>
<221> 变体
<222> (3)..(3)
<223> Xaa = 任何氨基酸
<400> 2
Tyr Gly Xaa Gly Val
1 5
<210> 3
<211> 135
<212> PRT
<213> 人工序列
<220>
<223> 杂合细菌素Ent35-MccV
<400> 3
Gly Lys Tyr Tyr Gly Asn Gly Val Ser Cys Asn Lys Lys Gly Cys Ser
1 5 10 15
Val Asp Trp Gly Arg Ala Ile Gly Ile Ile Gly Asn Asn Ser Ala Ala
20 25 30
Asn Leu Ala Thr Gly Gly Ala Ala Gly Trp Lys Ser Gly Gly Gly Ala
35 40 45
Ser Gly Arg Asp Ile Ala Met Ala Ile Gly Thr Leu Ser Gly Gln Phe
50 55 60
Val Ala Gly Gly Ile Gly Ala Ala Ala Gly Gly Val Ala Gly Gly Ala
65 70 75 80
Ile Tyr Asp Tyr Ala Ser Thr His Lys Pro Asn Pro Ala Met Ser Pro
85 90 95
Ser Gly Leu Gly Gly Thr Ile Lys Gln Lys Pro Glu Gly Ile Pro Ser
100 105 110
Glu Ala Trp Asn Tyr Ala Ala Gly Arg Leu Cys Asn Trp Ser Pro Asn
115 120 125
Asn Leu Ser Asp Val Cys Leu
130 135
<210> 4
<211> 46
<212> PRT
<213> 嗜酸乳杆菌
<400> 4
Met Ile Ser Ser His Gln Lys Thr Leu Thr Asp Lys Glu Leu Ala Leu
1 5 10 15
Ile Ser Gly Gly Lys Thr His Tyr Pro Thr Asn Ala Trp Lys Ser Leu
20 25 30
Trp Lys Gly Phe Trp Glu Ser Leu Arg Tyr Thr Asp Gly Phe
35 40 45
<210> 5
<211> 141
<212> DNA
<213> 嗜酸乳杆菌
<400> 5
atgatttcat ctcatcaaaa aacgttaact gataaagaat tagcattaat ttctgggggg 60
aaaacgcact acccgactaa tgcatggaaa agtctttgga aaggtttctg ggaaagcctt 120
cgttatactg acggttttta g 141
<210> 6
<211> 81
<212> PRT
<213> 嗜酸乳杆菌
<400> 6
Met Ile Ser Met Ile Ser Ser His Gln Lys Thr Leu Thr Asp Lys Glu
1 5 10 15
Leu Ala Leu Ile Ser Gly Gly Lys Thr Tyr Tyr Gly Thr Asn Gly Val
20 25 30
His Cys Thr Lys Lys Ser Leu Trp Gly Lys Val Arg Leu Lys Asn Val
35 40 45
Ile Pro Gly Thr Leu Cys Arg Lys Gln Ser Leu Pro Ile Lys Gln Asp
50 55 60
Leu Lys Ile Leu Leu Gly Trp Ala Thr Gly Ala Phe Gly Lys Thr Phe
65 70 75 80
His
<210> 7
<211> 246
<212> DNA
<213> 嗜酸乳杆菌
<400> 7
atgatttcaa tgatttcatc tcatcaaaaa acgttaactg ataaagaatt agcattaatt 60
tctgggggga aaacgtacta tggtactaat ggtgtgcatt gtactaaaaa gagtctttgg 120
ggtaaagtac gcttaaaaaa cgtgattcct ggaactcttt gtcgtaagca atcgttgccg 180
atcaaacagg atttaaaaat tttactgggc tgggctacag gtgcttttgg caagacattt 240
cattaa 246
<210> 8
<211> 60
<212> PRT
<213> 嗜酸乳杆菌
<400> 8
Met Asp Lys Lys Thr Lys Ile Leu Phe Glu Val Leu Tyr Ile Ile Cys
1 5 10 15
Ile Ile Gly Pro Gln Phe Ile Leu Phe Val Thr Ala Lys Asn Asn Met
20 25 30
Tyr Gln Leu Val Gly Ser Phe Val Gly Ile Val Trp Phe Ser Tyr Ile
35 40 45
Phe Trp Tyr Ile Phe Phe Lys Gln His Lys Lys Met
50 55 60
<210> 9
<211> 183
<212> DNA
<213> 嗜酸乳杆菌
<400> 9
atggataaga aaacaaaaat attatttgaa gtattataca tcatctgtat aataggccct 60
caatttatat tatttgtgac tgcaaaaaac aatatgtatc agttggtggg ttcgtttgtt 120
ggaatagtat ggttttcgta tattttttgg tatatttttt tcaaacaaca taaaaaaatg 180
tag 183
<210> 10
<211> 65
<212> PRT
<213> 加氏乳杆菌
<400> 10
Met Ala Leu Lys Thr Leu Glu Lys His Glu Leu Arg Asn Val Met Gly
1 5 10 15
Gly Asn Lys Trp Gly Asn Ala Val Ile Gly Ala Ala Thr Gly Ala Thr
20 25 30
Arg Gly Val Ser Trp Cys Arg Gly Phe Gly Pro Trp Gly Met Thr Ala
35 40 45
Cys Ala Leu Gly Gly Ala Ala Ile Gly Gly Tyr Leu Gly Tyr Lys Ser
50 55 60
Asn
65
<210> 11
<211> 198
<212> DNA
<213> 加氏乳杆菌
<400> 11
atggctttaa aaacattaga aaaacatgaa ttaagaaatg taatgggtgg aaacaagtgg 60
gggaatgctg taataggagc tgctacggga gctactcgcg gagtaagttg gtgcagagga 120
ttcggaccat ggggaatgac tgcctgtgcg ttaggaggtg ctgcaattgg aggatatctg 180
ggatataaga gtaattaa 198
<210> 12
<211> 51
<212> PRT
<213> 金黄色葡萄球菌
<400> 12
Met Ser Trp Leu Asn Phe Leu Lys Tyr Ile Ala Lys Tyr Gly Lys Lys
1 5 10 15
Ala Val Ser Ala Ala Trp Lys Tyr Lys Gly Lys Val Leu Glu Trp Leu
20 25 30
Asn Val Gly Pro Thr Leu Glu Trp Val Trp Gln Lys Leu Lys Lys Ile
35 40 45
Ala Gly Leu
50
<210> 13
<211> 156
<212> DNA
<213> 金黄色葡萄球菌
<400> 13
atgagttggt taaatttttt aaaatacatc gctaaatatg gcaaaaaagc ggtatctgct 60
gcttggaagt acaaaggtaa agtattagaa tggcttaatg ttggtcctac tcttgaatgg 120
gtatggcaaa aattaaagaa aattgctgga ttataa 156
<210> 14
<211> 61
<212> PRT
<213> 禽肠球菌
<400> 14
Met Thr Arg Ser Lys Lys Leu Asn Leu Arg Glu Met Lys Asn Val Val
1 5 10 15
Gly Gly Thr Tyr Tyr Gly Asn Gly Val Ser Cys Asn Lys Lys Gly Cys
20 25 30
Ser Val Asp Trp Gly Lys Ala Ile Ser Ile Ile Gly Asn Asn Ser Ala
35 40 45
Ala Asn Leu Ala Thr Gly Gly Ala Ala Gly Trp Lys Ser
50 55 60
<210> 15
<211> 186
<212> DNA
<213> 禽肠球菌
<400> 15
atgacaagat caaaaaaatt aaatttacgc gaaatgaaga atgttgttgg tggtacctac 60
tatggaaatg gtgtatcttg taacaagaaa ggctgttcag ttgactgggg caaagccatc 120
agtattatag gaaataattc cgcagcaaac ttagcaactg gtggtgctgc tggttggaag 180
tcataa 186
<210> 16
<211> 67
<212> PRT
<213> 粪肠球菌
<400> 16
Met Lys Lys Lys Leu Val Ile Cys Gly Ile Ile Gly Ile Gly Phe Thr
1 5 10 15
Ala Leu Gly Thr Asn Val Glu Ala Ala Thr Tyr Tyr Gly Asn Gly Leu
20 25 30
Tyr Cys Asn Lys Gln Lys Cys Trp Val Asp Trp Asn Lys Ala Ser Arg
35 40 45
Glu Ile Gly Lys Ile Ile Val Asn Gly Trp Val Gln His Gly Pro Trp
50 55 60
Ala Pro Arg
65
<210> 17
<211> 204
<212> DNA
<213> 粪肠球菌
<400> 17
atgaaaaaga aattagttat ttgtggcatt attgggattg gttttacagc attaggaaca 60
aatgtagaag ctgctacgta ttacggaaat ggtttatatt gtaataagca aaaatgttgg 120
gtagactgga ataaagcttc aagggaaatt ggaaaaatta ttgttaatgg ttgggtacaa 180
catggccctt gggctcctag atag 204
<210> 18
<211> 51
<212> PRT
<213> 乳酸乳球菌
<400> 18
Met Lys Glu Gln Asn Ser Phe Asn Leu Leu Gln Glu Val Thr Glu Ser
1 5 10 15
Glu Leu Asp Leu Ile Leu Gly Ala Lys Gly Gly Ser Gly Val Ile His
20 25 30
Thr Ile Ser His Glu Val Ile Tyr Asn Ser Trp Asn Phe Val Phe Thr
35 40 45
Cys Cys Ser
50
<210> 19
<211> 156
<212> DNA
<213> 乳酸乳球菌
<400> 19
atgaaagaac aaaactcttt taatcttctt caagaagtga cagaaagtga attggacctt 60
attttaggtg caaaaggcgg cagtggagtt attcatacaa tttctcatga agtaatatat 120
aatagctgga actttgtatt tacttgctgc tcttaa 156
<210> 20
<211> 74
<212> PRT
<213> 屎肠球菌
<400> 20
Met Lys Lys Lys Val Leu Lys His Cys Val Ile Leu Gly Ile Leu Gly
1 5 10 15
Thr Cys Leu Ala Gly Ile Gly Thr Gly Ile Lys Val Asp Ala Ala Thr
20 25 30
Tyr Tyr Gly Asn Gly Leu Tyr Cys Asn Lys Glu Lys Cys Trp Val Asp
35 40 45
Trp Asn Gln Ala Lys Gly Glu Ile Gly Lys Ile Ile Val Asn Gly Trp
50 55 60
Val Asn His Gly Pro Trp Ala Pro Arg Arg
65 70
<210> 21
<211> 225
<212> DNA
<213> 屎肠球菌
<400> 21
atgaaaaaga aagtattaaa acattgtgtt attctaggaa tattaggaac ttgtctagct 60
ggcatcggta caggaataaa agttgatgca gctacttact atggaaatgg tctttattgt 120
aacaaagaaa aatgttgggt agattggaat caagctaaag gagaaattgg aaaaattatt 180
gttaatggtt gggttaatca tggtccatgg gcacctagaa ggtag 225
<210> 22
<211> 50
<212> PRT
<213> 肉毒杆菌
<400> 22
Met Gln Lys Pro Glu Ile Ile Ser Ala Asp Leu Gly Leu Cys Ala Val
1 5 10 15
Asn Glu Phe Val Ala Leu Ala Ala Ile Pro Gly Gly Ala Ala Thr Phe
20 25 30
Ala Val Cys Gln Met Pro Asn Leu Asp Glu Ile Val Ser Asn Ala Ala
35 40 45
Tyr Val
50
<210> 23
<211> 153
<212> DNA
<213> 肉毒杆菌
<400> 23
atgcaaaaac cagaaattat tagtgctgat ttagggcttt gtgcagttaa tgaatttgta 60
gctcttgctg ccattcctgg tggtgctgct acatttgcag tatgccaaat gccaaacttg 120
gatgagattg ttagtaatgc agcatatgtt taa 153
<210> 24
<211> 58
<212> PRT
<213> 马链球菌
<400> 24
Met Met Asn Ala Thr Glu Asn Gln Ile Phe Val Glu Thr Val Ser Asp
1 5 10 15
Gln Glu Leu Glu Met Leu Ile Gly Gly Ala Asp Arg Gly Trp Ile Lys
20 25 30
Thr Leu Thr Lys Asp Cys Pro Asn Val Ile Ser Ser Ile Cys Ala Gly
35 40 45
Thr Ile Ile Thr Ala Cys Lys Asn Cys Ala
50 55
<210> 25
<211> 177
<212> DNA
<213> 马链球菌
<400> 25
atgatgaatg ctactgaaaa ccaaattttt gttgagactg tgagtgacca agaattagaa 60
atgttaattg gtggtgcaga tcgtggatgg attaagactt taacaaaaga ttgtccaaat 120
gtaatttctt caatttgtgc aggtacaatt attacagctt gtaaaaattg tgcttaa 177
<210> 26
<211> 77
<212> PRT
<213> 田野环丝菌
<400> 26
Met His Lys Val Lys Lys Leu Asn Asn Gln Glu Leu Gln Gln Ile Val
1 5 10 15
Gly Gly Tyr Ser Ser Lys Asp Cys Leu Lys Asp Ile Gly Lys Gly Ile
20 25 30
Gly Ala Gly Thr Val Ala Gly Ala Ala Gly Gly Gly Leu Ala Ala Gly
35 40 45
Leu Gly Ala Ile Pro Gly Ala Phe Val Gly Ala His Phe Gly Val Ile
50 55 60
Gly Gly Ser Ala Ala Cys Ile Gly Gly Leu Leu Gly Asn
65 70 75
<210> 27
<211> 234
<212> DNA
<213> 田野环丝菌
<400> 27
atgcacaagg taaaaaaatt aaacaatcaa gagttacaac agatcgtggg aggttacagt 60
tcaaaagatt gtctaaaaga tattggtaaa ggaattggtg ctggtacagt agctggggca 120
gccggcggtg gcctagctgc aggattaggt gctatcccag gagcattcgt tggagcacat 180
tttggagtaa tcggcggatc tgccgcatgc attggtggat tattaggtaa ctag 234
<210> 28
<211> 80
<212> PRT
<213> 溶纤维丁酸弧菌
<400> 28
Met Ser Lys Lys Gln Ile Met Ser Asn Cys Ile Ser Ile Ala Leu Leu
1 5 10 15
Ile Ala Leu Ile Pro Asn Ile Tyr Phe Ile Ala Asp Lys Met Gly Ile
20 25 30
Gln Leu Ala Pro Ala Trp Tyr Gln Asp Ile Val Asn Trp Val Ser Ala
35 40 45
Gly Gly Thr Leu Thr Thr Gly Phe Ala Ile Ile Val Gly Val Thr Val
50 55 60
Pro Ala Trp Ile Ala Glu Ala Ala Ala Ala Phe Gly Ile Ala Ser Ala
65 70 75 80
<210> 29
<211> 243
<212> DNA
<213> 溶纤维丁酸弧菌
<400> 29
atgagtaaaa aacaaattat gagtaactgt atatcaattg cattattaat agcactaatt 60
cctaatatct attttattgc agataaaatg ggaattcagt tagcacctgc ttggtatcaa 120
gatattgtga attgggtatc tgctggtgga acacttacta ctggttttgc gattattgta 180
ggagttacag taccggcatg gatagcagaa gcagctgcag cttttggtat agcttcagca 240
tga 243
<210> 30
<211> 48
<212> PRT
<213> 溶纤维丁酸弧菌
<400> 30
Met Asn Lys Glu Leu Asn Ala Leu Thr Asn Pro Ile Asp Glu Lys Glu
1 5 10 15
Leu Glu Gln Ile Leu Gly Gly Gly Asn Gly Val Ile Lys Thr Ile Ser
20 25 30
His Glu Cys His Met Asn Thr Trp Gln Phe Ile Phe Thr Cys Cys Ser
35 40 45
<210> 31
<211> 147
<212> DNA
<213> 溶纤维丁酸弧菌
<400> 31
atgaacaaag aacttaatgc acttacaaat cctattgacg agaaggagct tgagcagatc 60
ctcggtggtg gcaatggtgt catcaagaca atcagccacg agtgccacat gaacacatgg 120
cagttcattt tcacatgttg ctcttaa 147
<210> 32
<211> 66
<212> PRT
<213> 麦芽香肉杆菌
<400> 32
Met Asn Ser Val Lys Glu Leu Asn Val Lys Glu Met Lys Gln Leu His
1 5 10 15
Gly Gly Val Asn Tyr Gly Asn Gly Val Ser Cys Ser Lys Thr Lys Cys
20 25 30
Ser Val Asn Trp Gly Gln Ala Phe Gln Glu Arg Tyr Thr Ala Gly Ile
35 40 45
Asn Ser Phe Val Ser Gly Val Ala Ser Gly Ala Gly Ser Ile Gly Arg
50 55 60
Arg Pro
65
<210> 33
<211> 201
<212> DNA
<213> 麦芽香肉杆菌
<400> 33
atgaatagcg taaaagaatt aaacgtgaaa gaaatgaaac aattacacgg tggagtaaat 60
tatggtaatg gtgtttcttg cagtaaaaca aaatgttcag ttaactgggg acaagccttt 120
caagaaagat acacagctgg aattaactca tttgtaagtg gagtcgcttc tggggcagga 180
tccattggta ggagaccgta a 201
<210> 34
<211> 61
<212> PRT
<213> 麦芽香肉杆菌
<400> 34
Met Lys Ser Val Lys Glu Leu Asn Lys Lys Glu Met Gln Gln Ile Asn
1 5 10 15
Gly Gly Ala Ile Ser Tyr Gly Asn Gly Val Tyr Cys Asn Lys Glu Lys
20 25 30
Cys Trp Val Asn Lys Ala Glu Asn Lys Gln Ala Ile Thr Gly Ile Val
35 40 45
Ile Gly Gly Trp Ala Ser Ser Leu Ala Gly Met Gly His
50 55 60
<210> 35
<211> 186
<212> DNA
<213> 麦芽香肉杆菌
<400> 35
atgaaaagcg ttaaagaact aaataaaaaa gaaatgcaac aaattaatgg tggagctatc 60
tcttatggca atggtgttta ttgtaacaaa gagaaatgtt gggtaaacaa ggcagaaaac 120
aaacaagcta ttactggaat agttatcggt ggatgggctt ctagtttagc aggaatggga 180
cattaa 186
<210> 36
<211> 71
<212> PRT
<213> 麦芽香肉杆菌
<400> 36
Met Asn Asn Val Lys Glu Leu Ser Ile Lys Glu Met Gln Gln Val Thr
1 5 10 15
Gly Gly Asp Gln Met Ser Asp Gly Val Asn Tyr Gly Lys Gly Ser Ser
20 25 30
Leu Ser Lys Gly Gly Ala Lys Cys Gly Leu Gly Ile Val Gly Gly Leu
35 40 45
Ala Thr Ile Pro Ser Gly Pro Leu Gly Trp Leu Ala Gly Ala Ala Gly
50 55 60
Val Ile Asn Ser Cys Met Lys
65 70
<210> 37
<211> 216
<212> DNA
<213> 麦芽香肉杆菌
<400> 37
atgaataatg taaaagagtt aagtattaaa gaaatgcaac aagttactgg tggagaccaa 60
atgtcagatg gtgtaaatta tggaaaaggc tctagcttat caaaaggtgg tgccaaatgt 120
ggtttaggga tcgtcggcgg attagctact atcccttcag gtcctttagg ctggttagcc 180
ggagcagcag gtgtaattaa tagctgtatg aaataa 216
<210> 38
<211> 64
<212> PRT
<213> 麦芽香肉杆菌
<400> 38
Met Leu Tyr Glu Leu Val Ala Tyr Gly Ile Ala Gln Gly Thr Ala Glu
1 5 10 15
Lys Val Val Ser Leu Ile Asn Ala Gly Leu Thr Val Gly Ser Ile Ile
20 25 30
Ser Ile Leu Gly Gly Val Thr Val Gly Leu Ser Gly Val Phe Thr Ala
35 40 45
Val Lys Ala Ala Ile Ala Lys Gln Gly Ile Lys Lys Ala Ile Gln Leu
50 55 60
<210> 39
<211> 195
<212> DNA
<213> 麦芽香肉杆菌
<400> 39
atgttatatg aattagttgc atatggtatc gcacaaggta cagctgaaaa ggttgtaagt 60
ctaattaacg caggtttaac agtagggtct attatttcaa ttttgggtgg ggtcacagtc 120
ggtttatcag gtgtcttcac agcagttaaa gcagcaattg ctaaacaagg aataaaaaaa 180
gcaattcaat tataa 195
<210> 40
<211> 836
<212> PRT
<213> 胡萝卜软腐果胶杆菌胡萝卜亚种
<400> 40
Met Ile Lys Tyr Arg Leu Tyr Ala Pro Asn Asp Gly Asp Thr Met Thr
1 5 10 15
Val Ser Gly Gly Gly Gly Trp Val Ser Asn Asp Asp Arg Lys Gly Gly
20 25 30
Asn Asp Arg Asp Asn Gly Lys Gly Gly Ser Ala Val Asp Phe Ser Lys
35 40 45
Asn Pro Glu Lys Gln Ala Ile Val Asn Pro Tyr Leu Ala Ile Ala Ile
50 55 60
Pro Met Pro Val Tyr Pro Leu Tyr Gly Lys Leu Gly Phe Thr Ile Asn
65 70 75 80
Thr Thr Ala Ile Glu Thr Glu Leu Ala Asn Val Arg Ala Ala Ile Asn
85 90 95
Thr Lys Leu Ala Thr Leu Ser Ala Val Ile Gly Arg Ser Leu Pro Val
100 105 110
Val Gly Arg Val Phe Gly Val Thr Ala Ala Gly Met Trp Pro Ser Ser
115 120 125
Thr Ala Pro Ser Ser Leu Asp Ser Ile Tyr Asn Gln Ala His Gln Gln
130 135 140
Ala Leu Ala Gln Leu Ala Ala Gln Gln Gly Val Leu Asn Lys Gly Tyr
145 150 155 160
Asn Val Thr Ala Met Pro Ala Gly Phe Val Ser Ser Leu Pro Val Ser
165 170 175
Glu Ile Lys Ser Leu Pro Thr Ala Pro Ala Ser Leu Leu Ala Gln Ser
180 185 190
Val Ile Asn Thr Glu Leu Ser Gln Arg Gln Leu Ala Leu Thr Gln Pro
195 200 205
Thr Thr Asn Ala Pro Val Ala Asn Ile Pro Val Val Lys Ala Glu Lys
210 215 220
Thr Ala Met Pro Gly Val Tyr Ser Ala Lys Ile Ile Ala Gly Glu Pro
225 230 235 240
Ala Phe Gln Ile Lys Val Asp Asn Thr Lys Pro Ala Leu Ala Gln Asn
245 250 255
Pro Pro Lys Val Lys Asp Asp Ile Gln Val Ser Ser Phe Leu Ser Ser
260 265 270
Pro Val Ala Asp Thr His His Ala Phe Ile Asp Phe Gly Ser Asp His
275 280 285
Glu Pro Val Tyr Val Ser Leu Ser Lys Ile Val Thr Ala Glu Glu Glu
290 295 300
Lys Lys Gln Val Glu Glu Ala Lys Arg Arg Glu Gln Glu Trp Leu Leu
305 310 315 320
Arg His Pro Ile Thr Ala Ala Glu Arg Lys Leu Thr Glu Ile Arg Gln
325 330 335
Val Ile Ser Phe Ala Gln Gln Leu Lys Glu Ser Ser Val Ala Thr Ile
340 345 350
Ser Glu Lys Thr Lys Thr Val Ala Val Tyr Gln Glu Gln Val Asn Thr
355 360 365
Ala Ala Lys Asn Arg Asp Asn Phe Tyr Asn Gln Asn Arg Gly Leu Leu
370 375 380
Ser Ala Gly Ile Thr Gly Gly Pro Gly Tyr Pro Ile Tyr Leu Ala Leu
385 390 395 400
Trp Gln Thr Met Asn Asn Phe His Gln Ala Tyr Phe Arg Ala Asn Asn
405 410 415
Ala Leu Glu Gln Glu Ser His Val Leu Asn Leu Ala Arg Ser Asp Leu
420 425 430
Ala Lys Ala Glu Gln Leu Leu Ala Glu Asn Asn Arg Leu Gln Val Glu
435 440 445
Thr Glu Arg Thr Leu Ala Glu Glu Lys Glu Ile Lys Arg Asn Arg Val
450 455 460
Asn Val Ser Thr Phe Gly Thr Val Gln Thr Gln Leu Ser Lys Leu Leu
465 470 475 480
Ser Asp Phe Tyr Ala Val Thr Ser Leu Ser Gln Ser Val Pro Ser Gly
485 490 495
Ala Leu Ala Ser Phe Ser Tyr Asn Pro Gln Gly Met Ile Gly Ser Gly
500 505 510
Lys Ile Val Gly Lys Asp Val Asp Val Leu Phe Ser Ile Pro Val Lys
515 520 525
Asp Ile Pro Gly Tyr Lys Ser Pro Ile Asn Leu Asp Asp Leu Ala Lys
530 535 540
Lys Asn Gly Ser Leu Asp Leu Pro Ile Arg Leu Ala Phe Ser Asp Glu
545 550 555 560
Asn Gly Glu Arg Val Leu Arg Ala Phe Lys Ala Asp Ser Leu Arg Ile
565 570 575
Pro Ser Ser Val Arg Gly Val Ala Gly Ser Tyr Asp Lys Asn Thr Gly
580 585 590
Ile Phe Ser Ala Glu Ile Asp Gly Val Ser Ser Arg Leu Val Leu Glu
595 600 605
Asn Pro Ala Phe Pro Pro Thr Gly Asn Val Gly Asn Thr Gly Asn Thr
610 615 620
Ala Pro Asp Tyr Lys Ala Leu Leu Asn Thr Gly Val Asp Val Lys Pro
625 630 635 640
Val Asp Lys Ile Thr Val Thr Val Thr Pro Val Ala Asp Pro Val Asp
645 650 655
Ile Asp Asp Tyr Ile Ile Trp Leu Pro Thr Ala Ser Gly Ser Gly Val
660 665 670
Glu Pro Ile Tyr Val Val Phe Asn Ser Asn Pro Tyr Gly Gly Thr Glu
675 680 685
Lys Gly Lys Tyr Ser Lys Arg Tyr Tyr Asn Pro Asp Lys Ala Gly Gly
690 695 700
Pro Ile Leu Glu Leu Asp Trp Lys Asn Val Lys Ile Asp His Ala Gly
705 710 715 720
Val Asp Asn Val Lys Leu His Thr Gly Arg Phe Lys Ala Ser Val Glu
725 730 735
Asn Lys Val Met Ile Glu Arg Leu Glu Asn Ile Leu Asn Gly Gln Ile
740 745 750
Thr Ala Thr Asp Thr Asp Lys Arg Phe Tyr Thr His Glu Leu Arg Glu
755 760 765
Leu Asn Arg Tyr Arg Asn Leu Gly Ile Lys Asp Gly Glu Val Pro Ser
770 775 780
Ser Ile Gln Glu Glu Ser Ala Val Trp Asn Asp Thr His Thr Ala Thr
785 790 795 800
Leu Glu Asp Tyr Lys Ile Asn Glu Lys Glu Gln Pro Leu Tyr Thr Asp
805 810 815
Ala Ala Leu Gln Ala Ala Tyr Glu Gln Glu Leu Lys Asp Ala Leu Gly
820 825 830
Gly Lys His Gly
835
<210> 41
<211> 2511
<212> DNA
<213> 胡萝卜软腐果胶杆菌胡萝卜亚种
<400> 41
atgattaaat accgtttata tgctccaaat gatggagaca ccatgacagt gagtggtggt 60
ggtggttggg tttcaaacga tgatcgcaaa ggtggtaatg acagggacaa tggcaaaggt 120
ggttctgccg ttgattttag taaaaatcca gaaaagcagg ctatcgttaa tccctatttg 180
gcaatcgcga taccgatgcc ggtctaccct ctttatggaa agctagggtt cacaataaat 240
acgacggcaa ttgagactga actcgcaaat gtcagagcag caattaacac taaacttgca 300
acactcagtg cagtgattgg cagatcactt ccggtcgttg ggcgggtatt tggtgttact 360
gccgccggaa tgtggccttc tagtaccgct cccagtagtc tcgattctat atacaatcaa 420
gcacatcagc aggctttagc ccagttagct gctcaacagg gagtattaaa taaagggtat 480
aacgttacag caatgcctgc aggtttcgtc agcagtttgc ctgttagtga aatcaaatca 540
ttgccaacag ctcccgccag tttactggca caaagtgtga ttaataccga actttcccag 600
cgtcaactgg ctcttactca gcccacgacg aatgcaccag tcgcgaatat tcccgtagtt 660
aaagcagaga aaacagcaat gccaggtgtg tattcagcga aaattattgc tggtgagcct 720
gcattccaaa tcaaggtcga taataccaaa cctgctttgg cacagaatcc gccgaaagta 780
aaagatgata ttcaggtatc ttctttcctt tcctcgccag tagctgatac gcaccatgca 840
tttattgatt ttggcagcga tcatgaaccg gtatacgtgt ctctttcaaa gatcgtgaca 900
gccgaggagg agaaaaaaca ggttgaagag gccaagcgcc gtgagcagga gtggttgttg 960
cgtcatccaa ttacagctgc ggagcgaaaa ttaactgaaa tccgccaagt gatctctttt 1020
gctcaacagc taaaagaaag ctctgtcgca accatttcag aaaaaactaa aactgttgcg 1080
gtttaccaag aacaggtgaa taccgctgca aaaaatcgcg acaattttta taatcaaaat 1140
agaggtctgt taagtgcggg tataactggg ggaccgggat atcctattta tcttgcttta 1200
tggcaaacga tgaataactt tcatcaggct tatttcagag caaataatgc attggaacaa 1260
gagagtcatg ttctgaacct ggctcgttct gatctggcta aggctgagca attgcttgct 1320
gagaataatc gacttcaggt tgaaacggag cgaacgcttg ccgaagaaaa agagataaaa 1380
cgcaacaggg ttaatgtatc aacatttggc acagtgcaaa ctcaacttag taaattgctg 1440
tcagattttt atgctgttac atcactttcc caaagtgttc cttcgggggc attagcctct 1500
ttttcatata atccacaagg gatgattggc agcggtaaga ttgttgggaa ggatgtcgat 1560
gttttatttt ccatcccagt aaaagatatt ccgggatata aatctcctat taacttggac 1620
gatttagcca agaaaaatgg aagtctggat cttcccattc gtctggcatt ttctgatgag 1680
aatggagaaa gggttcttcg ggcattcaaa gcggatagtc tgcgaatccc ttcgagtgtc 1740
agaggtgtag cgggcagtta tgacaaaaat acgggtattt ttagtgcaga aattgatggt 1800
gtttcatctc gccttgtact ggaaaaccca gcgtttcctc cgaccggaaa tgtcggtaat 1860
acgggtaata ctgcacctga ctataaagca ttactgaata ctggtgttga tgttaaacct 1920
gttgataaaa tcacagttac ggtaacacca gttgctgatc cagtggatat tgatgactat 1980
ataatctggt tgccaactgc gtctggttct ggcgtggaac ccatttatgt cgtgtttaac 2040
agtaatccgt atggtgggac ggaaaaagga aaatatagca aacgttatta taatccagat 2100
aaggcaggcg gtccgatctt ggagctggat tggaaaaacg ttaagattga ccatgcaggt 2160
gtggacaatg ttaaattaca cacagggcgt ttcaaagcgt cggttgaaaa caaagtgatg 2220
attgaacgtt tggaaaacat actgaatggt caaatcacgg ccacggatac tgacaagcga 2280
ttctatacgc atgaattaag agagttaaac cgctacagaa atttaggcat caaagacggt 2340
gaagtgccta gtagcattca agaagaaagc gctgtttgga acgacacaca cacagcgacg 2400
cttgaagact acaaaattaa tgagaaagag caaccgttgt acactgatgc tgctttgcag 2460
gcagcctacg aacaggaact caaagacgca ttaggaggga aacatggcta a 2511
<210> 42
<211> 74
<212> PRT
<213> 蜡样芽胞杆菌
<400> 42
Met Glu Asn Leu Gln Met Leu Thr Glu Glu Glu Leu Met Glu Ile Glu
1 5 10 15
Gly Gly Gly Trp Trp Asn Ser Trp Gly Lys Cys Val Ala Gly Thr Ile
20 25 30
Gly Gly Ala Gly Thr Gly Gly Leu Gly Gly Ala Ala Ala Gly Ser Ala
35 40 45
Val Pro Val Ile Gly Thr Gly Ile Gly Gly Ala Ile Gly Gly Val Ser
50 55 60
Gly Gly Leu Thr Gly Ala Ala Thr Phe Cys
65 70
<210> 43
<211> 225
<212> DNA
<213> 蜡样芽胞杆菌
<400> 43
atggaaaact tacaaatgtt aactgaagaa gaattaatgg aaattgaagg tggaggctgg 60
tggaatagct ggggtaaatg tgttgctgga actatcggtg gagctggaac tggtggttta 120
ggtggagctg ctgcaggttc agctgttccg gttattggta ctggtattgg tggcgctatt 180
ggtggagtta gcggtggcct tacaggtgca gctacttttt gctaa 225
<210> 44
<211> 78
<212> PRT
<213> 灰轮丝链轮丝菌
<400> 44
Met Thr Ala Ser Ile Leu Gln Gln Ser Val Val Asp Ala Asp Phe Arg
1 5 10 15
Ala Ala Leu Leu Glu Asn Pro Ala Ala Phe Gly Ala Ser Ala Ala Ala
20 25 30
Leu Pro Thr Pro Val Glu Ala Gln Asp Gln Ala Ser Leu Asp Phe Trp
35 40 45
Thr Lys Asp Ile Ala Ala Thr Glu Ala Phe Ala Cys Arg Gln Ser Cys
50 55 60
Ser Phe Gly Pro Phe Thr Phe Val Cys Asp Gly Asn Thr Lys
65 70 75
<210> 45
<211> 237
<212> DNA
<213> 灰轮丝链轮丝菌
<400> 45
atgaccgctt ccattcttca gcagtccgtc gtggacgccg acttccgcgc ggcgctgctt 60
gagaaccccg ccgccttcgg cgcttccgcc gcggccctgc ccacgcccgt cgaggcccag 120
gaccaggcgt cccttgactt ctggaccaag gacatcgccg ccacggaagc cttcgcctgc 180
cgccagagct gcagcttcgg cccgttcacc ttcgtgtgcg acggcaacac caagtaa 237
<210> 46
<211> 76
<212> PRT
<213> 嗜高温细菌
<400> 46
Met Ser Leu Leu Ala Leu Val Ala Gly Thr Leu Gly Val Ser Gln Ser
1 5 10 15
Ile Ala Thr Thr Val Val Ser Ile Val Leu Thr Gly Ser Thr Leu Ile
20 25 30
Ser Ile Ile Leu Gly Ile Thr Ala Ile Leu Ser Gly Gly Val Asp Ala
35 40 45
Ile Leu Glu Ile Gly Trp Ser Ala Phe Val Ala Thr Val Lys Lys Ile
50 55 60
Val Ala Glu Arg Gly Lys Ala Ala Ala Ile Ala Trp
65 70 75
<210> 47
<211> 231
<212> DNA
<213> 嗜高温细菌
<400> 47
atgagtttgc tggcgcttgt tgccgggacg ctcggcgtgt cacagtcaat cgcgacgacg 60
gttgtttcga ttgtgttgac cggctccact ctcatttcta ttattcttgg gatcaccgct 120
attttgtcag gtggagtcga cgccattttg gaaattgggt ggtcagcttt tgtcgcgacg 180
gtgaaaaaaa tagtggcgga acgaggaaaa gcggcagcga ttgcatggta a 231
<210> 48
<211> 309
<212> PRT
<213> 酪丁酸梭菌
<400> 48
Met Arg Lys Val Phe Leu Arg Ser Ile Ile Ser Thr Leu Val Met Cys
1 5 10 15
Ala Phe Val Ser Ser Ser Phe Ser Val Asn Ala Asp Glu Ser Lys Pro
20 25 30
Asn Asp Glu Lys Ile Ile Asn Asn Ile Glu Asn Val Thr Thr Thr Lys
35 40 45
Asp Ile Val Lys Ser Asn Lys Asn Asn Ile Val Tyr Leu Asp Glu Gly
50 55 60
Val Met Ser Ile Pro Leu Ser Gly Arg Lys Pro Ile Ala Ile Lys Asp
65 70 75 80
Asp Asn Asn Lys Glu Asp Leu Thr Val Thr Leu Pro Ile Lys Asn Thr
85 90 95
Gly Asp Ile Ser Lys Ile Ser Ser Asn Gly Thr Ile Leu Tyr Lys Asn
100 105 110
Asn Ser Ser Asn Ser Ser Asn Ile Ala Leu Gln Pro Lys Asn Asp Gly
115 120 125
Phe Lys Ala Leu Ile Asn Ile Asn Asp Lys Leu Ala Asn Lys Glu Tyr
130 135 140
Glu Phe Thr Phe Asn Leu Pro Lys Asn Ser Lys Leu Ile Ser Ala Ala
145 150 155 160
Thr Tyr Leu Gly Lys Glu Tyr Asp Thr Lys Glu Val Phe Val Val Asp
165 170 175
Lys Asn Asn Ile Ile Thr Ser Ile Ile Ser Pro Ala Trp Ala Lys Asp
180 185 190
Ala Asn Gly His Asn Val Ser Thr Tyr Tyr Lys Ile Val Ser Asn Asn
195 200 205
Lys Leu Val Gln Val Val Glu Phe Thr Glu Asn Thr Ala Phe Pro Val
210 215 220
Val Ala Asp Pro Asn Trp Thr Lys Ile Gly Lys Cys Ala Gly Ser Ile
225 230 235 240
Ala Trp Ala Ile Gly Ser Gly Leu Phe Gly Gly Ala Lys Leu Ile Lys
245 250 255
Ile Lys Lys Tyr Ile Ala Glu Leu Gly Gly Leu Gln Lys Ala Ala Lys
260 265 270
Leu Leu Val Gly Ala Thr Thr Trp Glu Glu Lys Leu His Ala Gly Gly
275 280 285
Tyr Ala Leu Ile Asn Leu Ala Ala Glu Leu Thr Gly Val Ala Gly Ile
290 295 300
Gln Ala Asn Cys Phe
305
<210> 49
<211> 930
<212> DNA
<213> 酪丁酸梭菌
<400> 49
ttgagaaaag tatttttaag atcaataatt tcaacattag ttatgtgtgc atttgtttca 60
agcagctttt cagtaaatgc ggatgaaagc aaaccaaatg atgaaaaaat aattaataac 120
atagaaaacg ttactactac taaagatatt gtaaaaagta ataaaaataa tattgtatat 180
ttagatgaag gtgtaatgag tattccattg tctgggagaa aacccattgc tattaaagat 240
gataataata aagaagattt aactgttaca ttacctatta agaatactgg agatatatct 300
aaaattagta gtaatggtac tattctgtat aaaaataata gtagtaattc atctaatata 360
gctttacaac ctaaaaatga tggatttaag gctttaataa atattaatga taagttagct 420
aataaagaat atgaatttac atttaattta cccaaaaaca gtaaattaat tagtgctgcc 480
acatatttgg gtaaagaata tgatacaaaa gaagtatttg tagtagacaa aaataatata 540
attacgagta ttattagtcc agcttgggct aaagatgcaa atggacataa tgtttctact 600
tattataaga tagtatcgaa taataaatta gtacaagttg ttgaattcac agaaaatact 660
gcattcccgg tggtagctga tcctaattgg actaaaattg ggaaatgcgc tgggtcaata 720
gcatgggcta taggttctgg cctttttggt ggagcaaagc taattaaaat aaaaaaatat 780
atagcagagc ttggaggact tcaaaaagca gctaaattat tagttggtgc aaccacttgg 840
gaagaaaaat tacacgcagg cggttatgca ttaattaact tagctgctga gctaacaggt 900
gtagcaggta tacaagcaaa ttgtttttaa 930
<210> 50
<211> 62
<212> PRT
<213> 凝结芽孢杆菌
<400> 50
Met Lys Lys Ile Glu Lys Leu Thr Glu Lys Glu Met Ala Asn Ile Ile
1 5 10 15
Gly Gly Lys Tyr Tyr Gly Asn Gly Val Thr Cys Gly Lys His Ser Cys
20 25 30
Ser Val Asp Trp Gly Lys Ala Thr Thr Cys Ile Ile Asn Asn Gly Ala
35 40 45
Met Ala Trp Ala Thr Gly Gly His Gln Gly Thr His Lys Cys
50 55 60
<210> 51
<211> 189
<212> DNA
<213> 凝结芽孢杆菌
<400> 51
atgaaaaaaa ttgaaaaatt aactgaaaaa gaaatggcca atatcattgg tggtaaatac 60
tacggtaatg gggttacttg tggcaaacat tcctgctctg ttgactgggg taaggctacc 120
acctgcataa tcaataatgg agctatggca tgggctactg gtggacatca aggtactcat 180
aaatgctag 189
<210> 52
<211> 490
<212> PRT
<213> 大肠杆菌
<400> 52
Met Asp Lys Val Thr Asp Asn Ser Pro Asp Val Glu Ser Thr Glu Ser
1 5 10 15
Thr Glu Gly Ser Phe Pro Thr Val Gly Val Asp Thr Gly Asp Thr Ile
20 25 30
Thr Ala Thr Leu Ala Thr Gly Thr Glu Asn Val Gly Gly Gly Gly Gly
35 40 45
Ala Phe Gly Gly Ala Ser Glu Ser Ser Ala Ala Ile His Ala Thr Ala
50 55 60
Lys Trp Ser Thr Ala Gln Leu Lys Lys His Gln Ala Glu Gln Ala Ala
65 70 75 80
Arg Ala Ala Ala Ala Glu Ala Ala Leu Ala Lys Ala Lys Ser Gln Arg
85 90 95
Asp Ala Leu Thr Gln Arg Leu Lys Asp Ile Val Asn Asp Ala Leu Arg
100 105 110
Ala Asn Ala Ala Arg Ser Pro Ser Val Thr Asp Leu Ala His Ala Asn
115 120 125
Asn Met Ala Met Gln Ala Glu Ala Glu Arg Leu Arg Leu Ala Lys Ala
130 135 140
Glu Gln Lys Ala Arg Glu Glu Ala Glu Ala Ala Glu Lys Ala Leu Arg
145 150 155 160
Glu Ala Glu Arg Gln Arg Asp Glu Ile Ala Arg Gln Gln Ala Glu Thr
165 170 175
Ala His Leu Leu Ala Met Ala Glu Ala Ala Glu Ala Glu Lys Asn Arg
180 185 190
Gln Asp Ser Leu Asp Glu Glu His Arg Ala Val Glu Val Ala Glu Lys
195 200 205
Lys Leu Ala Glu Ala Lys Ala Glu Leu Ala Lys Ala Glu Ser Asp Val
210 215 220
Gln Ser Lys Gln Ala Ile Val Ser Arg Val Ala Gly Glu Leu Glu Asn
225 230 235 240
Ala Gln Lys Ser Val Asp Val Lys Val Thr Gly Phe Pro Gly Trp Arg
245 250 255
Asp Val Gln Lys Lys Leu Glu Arg Gln Leu Gln Asp Lys Lys Asn Glu
260 265 270
Tyr Ser Ser Val Thr Asn Ala Leu Asn Ser Ala Val Ser Ile Arg Asp
275 280 285
Ala Lys Lys Thr Glu Val Gln Asn Ala Glu Ile Lys Leu Lys Glu Ala
290 295 300
Lys Asp Ala Leu Glu Lys Ser Gln Val Lys Asp Ser Val Asp Thr Met
305 310 315 320
Val Gly Phe Tyr Gln Tyr Ile Thr Glu Gln Tyr Gly Glu Lys Tyr Ser
325 330 335
Arg Ile Ala Gln Asp Leu Ala Glu Lys Ala Lys Gly Ser Lys Phe Asn
340 345 350
Ser Val Asp Glu Ala Leu Ala Ala Phe Glu Lys Tyr Lys Asn Val Leu
355 360 365
Asp Lys Lys Phe Ser Lys Val Asp Arg Asp Asp Ile Phe Asn Ala Leu
370 375 380
Glu Ser Ile Thr Tyr Asp Glu Trp Ala Lys His Leu Glu Lys Ile Ser
385 390 395 400
Arg Ala Leu Lys Val Thr Gly Tyr Leu Ser Phe Gly Tyr Asp Val Trp
405 410 415
Asp Gly Thr Leu Lys Gly Leu Lys Thr Gly Asp Trp Lys Pro Leu Phe
420 425 430
Val Thr Leu Glu Lys Ser Ala Val Asp Phe Gly Val Ala Lys Ile Val
435 440 445
Ala Leu Met Phe Ser Phe Ile Val Gly Ala Pro Leu Gly Phe Trp Gly
450 455 460
Ile Ala Ile Ile Thr Gly Ile Val Ser Ser Tyr Ile Gly Asp Asp Glu
465 470 475 480
Leu Asn Lys Leu Asn Glu Leu Leu Gly Ile
485 490
<210> 53
<211> 1473
<212> DNA
<213> 大肠杆菌
<400> 53
atggataaag tcactgataa ttctccagat gtggagagca cagaatctac tgaggggtca 60
ttcccaactg ttggggttga tactggcgat acgattacag cgacgcttgc aactggaact 120
gaaaatgttg gtggaggcgg tggagcattt ggtggggcca gtgaaagttc tgctgcgata 180
catgcaaccg ctaaatggtc taccgcgcag ttgaaaaaac atcaggctga acaggctgcc 240
cgtgctgctg cggctgaggc agcattggca aaagcgaaat ctcagcgtga tgccctgact 300
caacgtctca aggatattgt taatgacgct ttacgtgcta atgccgctcg tagtccatca 360
gtaactgacc ttgctcatgc caataatatg gcaatgcagg cagaggctga gcgtttgcgc 420
cttgcgaagg cagagcaaaa agcccgtgaa gaagctgaag cagcagaaaa agcgctccgg 480
gaagcagaac gccaacgtga tgagattgcc cgccaacagg ctgaaaccgc gcatttgtta 540
gcaatggcgg aggcagcaga ggctgagaaa aatcgacagg attctcttga tgaagagcat 600
cgggctgtgg aagtggcaga gaagaagctg gctgaggcta aagctgaact ggcgaaggcc 660
gaaagcgatg tacagagtaa gcaagcgatt gtttccagag ttgcagggga gcttgaaaac 720
gctcaaaaaa gtgttgatgt gaaggttacc ggatttcctg gatggcgtga tgttcagaaa 780
aaactggaga gacaattgca ggataagaag aatgaatatt cgtcagtgac gaatgctctt 840
aattctgctg ttagcattag agatgctaaa aaaacagaag ttcagaatgc tgagataaaa 900
ttaaaagaag ctaaggatgc tcttgagaag agtcaggtaa aagactctgt tgatactatg 960
gttgggtttt atcaatatat aaccgaacaa tatggggaaa aatattccag aatagctcag 1020
gatttagctg aaaaggcgaa gggtagtaaa tttaatagtg ttgatgaagc acttgctgca 1080
tttgaaaagt ataaaaatgt actggataag aaattcagta aggttgatag ggatgatatt 1140
tttaatgctt tagagtctat tacttatgat gagtgggcca agcatctaga aaagatctct 1200
agggctctta aggttactgg atatttgtct ttcgggtatg atgtatggga tggtacccta 1260
aagggattaa aaacaggaga ctggaagcct ttatttgtca ctctggagaa gagcgcggta 1320
gatttcggcg tggcaaaaat tgtggcatta atgtttagtt ttattgttgg tgcgcctctt 1380
ggcttctggg gaattgcaat tatcacaggt attgtttctt cttacatagg ggatgatgag 1440
ttgaacaagc ttaatgaatt actaggtatt taa 1473
<210> 54
<211> 522
<212> PRT
<213> 大肠杆菌
<400> 54
Met Glu Thr Ala Val Ala Tyr Tyr Lys Asp Gly Val Pro Tyr Asp Asp
1 5 10 15
Lys Gly Gln Val Ile Ile Thr Leu Leu Asn Gly Thr Pro Asp Gly Ser
20 25 30
Gly Ser Gly Gly Gly Gly Gly Lys Gly Gly Ser Lys Ser Glu Ser Ser
35 40 45
Ala Ala Ile His Ala Thr Ala Lys Trp Ser Thr Ala Gln Leu Lys Lys
50 55 60
Thr Gln Ala Glu Gln Ala Ala Arg Ala Lys Ala Ala Ala Glu Ala Gln
65 70 75 80
Ala Lys Ala Lys Ala Asn Arg Asp Ala Leu Thr Gln Arg Leu Lys Asp
85 90 95
Ile Val Asn Glu Ala Leu Arg His Asn Ala Ser Arg Thr Pro Ser Ala
100 105 110
Thr Glu Leu Ala His Ala Asn Asn Ala Ala Met Gln Ala Glu Asp Glu
115 120 125
Arg Leu Arg Leu Ala Lys Ala Glu Glu Lys Ala Arg Lys Glu Ala Glu
130 135 140
Ala Ala Glu Lys Ala Phe Gln Glu Ala Glu Gln Arg Arg Lys Glu Ile
145 150 155 160
Glu Arg Glu Lys Ala Glu Thr Glu Arg Gln Leu Lys Leu Ala Glu Ala
165 170 175
Glu Glu Lys Arg Leu Ala Ala Leu Ser Glu Glu Ala Lys Ala Val Glu
180 185 190
Ile Ala Gln Lys Lys Leu Ser Ala Ala Gln Ser Glu Val Val Lys Met
195 200 205
Asp Gly Glu Ile Lys Thr Leu Asn Ser Arg Leu Ser Ser Ser Ile His
210 215 220
Ala Arg Asp Ala Glu Met Lys Thr Leu Ala Gly Lys Arg Asn Glu Leu
225 230 235 240
Ala Gln Ala Ser Ala Lys Tyr Lys Glu Leu Asp Glu Leu Val Lys Lys
245 250 255
Leu Ser Pro Arg Ala Asn Asp Pro Leu Gln Asn Arg Pro Phe Phe Glu
260 265 270
Ala Thr Arg Arg Arg Val Gly Ala Gly Lys Ile Arg Glu Glu Lys Gln
275 280 285
Lys Gln Val Thr Ala Ser Glu Thr Arg Ile Asn Arg Ile Asn Ala Asp
290 295 300
Ile Thr Gln Ile Gln Lys Ala Ile Ser Gln Val Ser Asn Asn Arg Asn
305 310 315 320
Ala Gly Ile Ala Arg Val His Glu Ala Glu Glu Asn Leu Lys Lys Ala
325 330 335
Gln Asn Asn Leu Leu Asn Ser Gln Ile Lys Asp Ala Val Asp Ala Thr
340 345 350
Val Ser Phe Tyr Gln Thr Leu Thr Glu Lys Tyr Gly Glu Lys Tyr Ser
355 360 365
Lys Met Ala Gln Glu Leu Ala Asp Lys Ser Lys Gly Lys Lys Ile Gly
370 375 380
Asn Val Asn Glu Ala Leu Ala Ala Phe Glu Lys Tyr Lys Asp Val Leu
385 390 395 400
Asn Lys Lys Phe Ser Lys Ala Asp Arg Asp Ala Ile Phe Asn Ala Leu
405 410 415
Ala Ser Val Lys Tyr Asp Asp Trp Ala Lys His Leu Asp Gln Phe Ala
420 425 430
Lys Tyr Leu Lys Ile Thr Gly His Val Ser Phe Gly Tyr Asp Val Val
435 440 445
Ser Asp Ile Leu Lys Ile Lys Asp Thr Gly Asp Trp Lys Pro Leu Phe
450 455 460
Leu Thr Leu Glu Lys Lys Ala Ala Asp Ala Gly Val Ser Tyr Val Val
465 470 475 480
Ala Leu Leu Phe Ser Leu Leu Ala Gly Thr Thr Leu Gly Ile Trp Gly
485 490 495
Ile Ala Ile Val Thr Gly Ile Leu Cys Ser Tyr Ile Asp Lys Asn Lys
500 505 510
Leu Asn Thr Ile Asn Glu Val Leu Gly Ile
515 520
<210> 55
<211> 1569
<212> DNA
<213> 大肠杆菌
<400> 55
atggaaaccg cggtagcgta ctataaagat ggtgttcctt atgatgataa gggacaggta 60
attattactc ttttgaatgg tactcctgac gggagtggct ctggcggcgg aggtggaaaa 120
ggaggcagta aaagtgaaag ttctgcagct attcatgcaa ctgctaaatg gtctactgct 180
caattaaaga aaacacaggc agagcaggct gcccgggcaa aagctgcagc ggaagcacag 240
gcgaaagcaa aggcaaacag ggatgcgctg actcagcgcc tgaaggatat cgtgaatgag 300
gctcttcgtc acaatgcctc acgtacgcct tcagcaacag agcttgctca tgctaataat 360
gcagctatgc aggcggaaga cgagcgtttg cgccttgcga aagcagaaga aaaagcccgt 420
aaagaagcgg aagcagcaga aaaggctttt caggaagcag aacaacgacg taaagagatt 480
gaacgggaga aggctgaaac agaacgccag ttgaaactgg ctgaagctga agagaaacga 540
ctggctgcat tgagtgaaga agctaaagct gttgagatcg cccaaaaaaa actttctgct 600
gcacaatctg aagtggtgaa aatggatgga gagattaaga ctctcaattc tcgtttaagc 660
tccagtatcc atgcccgtga tgcagaaatg aaaacgctcg ctggaaaacg aaatgaactg 720
gctcaggcat ccgctaaata taaagaactg gatgagctgg tcaaaaaact atcaccaaga 780
gccaatgatc cgcttcagaa ccgtcctttt tttgaagcaa ccagacgacg ggttggggcc 840
ggtaagatta gagaagaaaa acaaaaacag gtaacagcat cagaaacacg tattaaccgg 900
ataaatgctg atataactca gatccagaag gctatttctc aggtcagtaa taatcgtaat 960
gccggtatcg ctcgtgttca tgaagctgaa gaaaatttga aaaaagcaca gaataatctc 1020
cttaattcac agattaagga tgctgttgat gcaacagtta gcttttatca aacgctgact 1080
gaaaaatatg gtgaaaaata ttcgaaaatg gcacaggaac ttgctgataa gtctaaaggt 1140
aagaaaatcg gcaatgtgaa tgaagctctc gctgcttttg aaaaatacaa ggatgtttta 1200
aataagaaat tcagcaaagc cgatcgtgat gctattttta atgcgttggc atcggtgaag 1260
tatgatgact gggctaaaca tttagatcag tttgccaagt acttgaagat tacggggcat 1320
gtttcttttg gatatgatgt ggtatctgat atcctaaaaa ttaaggatac aggtgactgg 1380
aagccactat ttcttacatt agagaagaaa gctgcagatg caggggtgag ttatgttgtt 1440
gctttacttt ttagcttgct tgctggaact acattaggta tttggggtat tgctattgtt 1500
acaggaattc tatgctccta tattgataag aataaactta atactataaa tgaggtgtta 1560
gggatttaa 1569
<210> 56
<211> 626
<212> PRT
<213> 大肠杆菌
<400> 56
Met Ser Asp Pro Val Arg Ile Thr Asn Pro Gly Ala Glu Ser Leu Gly
1 5 10 15
Tyr Asp Ser Asp Gly His Glu Ile Met Ala Val Asp Ile Tyr Val Asn
20 25 30
Pro Pro Arg Val Asp Val Phe His Gly Thr Pro Pro Ala Trp Ser Ser
35 40 45
Phe Gly Asn Lys Thr Ile Trp Gly Gly Asn Glu Trp Val Asp Asp Ser
50 55 60
Pro Thr Arg Ser Asp Ile Glu Lys Arg Asp Lys Glu Ile Thr Ala Tyr
65 70 75 80
Lys Asn Thr Leu Ser Ala Gln Gln Lys Glu Asn Glu Asn Lys Arg Thr
85 90 95
Glu Ala Gly Lys Arg Leu Ser Ala Ala Ile Ala Ala Arg Glu Lys Asp
100 105 110
Glu Asn Thr Leu Lys Thr Leu Arg Ala Gly Asn Ala Asp Ala Ala Asp
115 120 125
Ile Thr Arg Gln Glu Phe Arg Leu Leu Gln Ala Glu Leu Arg Glu Tyr
130 135 140
Gly Phe Arg Thr Glu Ile Ala Gly Tyr Asp Ala Leu Arg Leu His Thr
145 150 155 160
Glu Ser Arg Met Leu Phe Ala Asp Ala Asp Ser Leu Arg Ile Ser Pro
165 170 175
Arg Glu Ala Arg Ser Leu Ile Glu Gln Ala Glu Lys Arg Gln Lys Asp
180 185 190
Ala Gln Asn Ala Asp Lys Lys Ala Ala Asp Met Leu Ala Glu Tyr Glu
195 200 205
Arg Arg Lys Gly Ile Leu Asp Thr Arg Leu Ser Glu Leu Glu Lys Asn
210 215 220
Gly Gly Ala Ala Leu Ala Val Leu Asp Ala Gln Gln Ala Arg Leu Leu
225 230 235 240
Gly Gln Gln Thr Arg Asn Asp Arg Ala Ile Ser Glu Ala Arg Asn Lys
245 250 255
Leu Ser Ser Val Thr Glu Ser Leu Asn Thr Ala Arg Asn Ala Leu Thr
260 265 270
Arg Ala Glu Gln Gln Leu Thr Gln Gln Lys Asn Thr Pro Asp Gly Lys
275 280 285
Thr Ile Val Ser Pro Glu Lys Phe Pro Gly Arg Ser Ser Thr Asn His
290 295 300
Ser Ile Val Val Ser Gly Asp Pro Arg Phe Ala Gly Thr Ile Lys Ile
305 310 315 320
Thr Thr Ser Ala Val Ile Asp Asn Arg Ala Asn Leu Asn Tyr Leu Leu
325 330 335
Ser His Ser Gly Leu Asp Tyr Lys Arg Asn Ile Leu Asn Asp Arg Asn
340 345 350
Pro Val Val Thr Glu Asp Val Glu Gly Asp Lys Lys Ile Tyr Asn Ala
355 360 365
Glu Val Ala Glu Trp Asp Lys Leu Arg Gln Arg Leu Leu Asp Ala Arg
370 375 380
Asn Lys Ile Thr Ser Ala Glu Ser Ala Val Asn Ser Ala Arg Asn Asn
385 390 395 400
Leu Ser Ala Arg Thr Asn Glu Gln Lys His Ala Asn Asp Ala Leu Asn
405 410 415
Ala Leu Leu Lys Glu Lys Glu Asn Ile Arg Asn Gln Leu Ser Gly Ile
420 425 430
Asn Gln Lys Ile Ala Glu Glu Lys Arg Lys Gln Asp Glu Leu Lys Ala
435 440 445
Thr Lys Asp Ala Ile Asn Phe Thr Thr Glu Phe Leu Lys Ser Val Ser
450 455 460
Glu Lys Tyr Gly Ala Lys Ala Glu Gln Leu Ala Arg Glu Met Ala Gly
465 470 475 480
Gln Ala Lys Gly Lys Lys Ile Arg Asn Val Glu Glu Ala Leu Lys Thr
485 490 495
Tyr Glu Lys Tyr Arg Ala Asp Ile Asn Lys Lys Ile Asn Ala Lys Asp
500 505 510
Arg Ala Ala Ile Ala Ala Ala Leu Glu Ser Val Lys Leu Ser Asp Ile
515 520 525
Ser Ser Asn Leu Asn Arg Phe Ser Arg Gly Leu Gly Tyr Ala Gly Lys
530 535 540
Phe Thr Ser Leu Ala Asp Trp Ile Thr Glu Phe Gly Lys Ala Val Arg
545 550 555 560
Thr Glu Asn Trp Arg Pro Leu Phe Val Lys Thr Glu Thr Ile Ile Ala
565 570 575
Gly Asn Ala Ala Thr Ala Leu Val Ala Leu Val Phe Ser Ile Leu Thr
580 585 590
Gly Ser Ala Leu Gly Ile Ile Gly Tyr Gly Leu Leu Met Ala Val Thr
595 600 605
Gly Ala Leu Ile Asp Glu Ser Leu Val Glu Lys Ala Asn Lys Phe Trp
610 615 620
Gly Ile
625
<210> 57
<211> 1881
<212> DNA
<213> 大肠杆菌
<400> 57
atgtctgacc ctgtacgtat tacaaatccc ggtgcagaat cgctggggta tgattcagat 60
ggccatgaaa ttatggccgt tgatatttat gtaaaccctc cacgtgtcga tgtctttcat 120
ggtaccccgc ctgcatggag ttccttcggg aacaaaacca tctggggcgg aaacgagtgg 180
gttgatgatt ccccaacccg aagtgatatc gaaaaaaggg acaaggaaat cacagcgtac 240
aaaaacacgc tcagcgcgca gcagaaagag aatgagaata agcgtactga agccggaaaa 300
cgcctctctg cggcgattgc tgcaagggaa aaagatgaaa acacactgaa aacactccgt 360
gccggaaacg cagatgccgc tgatattaca cgacaggagt tcagactcct gcaggcagag 420
ctgagagaat acggattccg tactgaaatc gccggatatg acgccctccg gctgcataca 480
gagagccgga tgctgtttgc tgatgctgat tctcttcgta tatctccccg ggaggccagg 540
tcgttaatcg aacaggctga aaaacggcag aaggatgcgc agaacgcaga caagaaggcc 600
gctgatatgc ttgctgaata cgagcgcaga aaaggtattc tggacacccg gttgtcagag 660
ctggaaaaaa atggcggggc agcccttgcc gttcttgatg cacaacaggc ccgtctgctc 720
gggcagcaga cacggaatga cagggccatt tcagaggccc ggaataaact cagttcagtg 780
acggaatcgc ttaacacggc ccgtaatgca ttaaccagag ctgaacaaca gctgacgcaa 840
cagaaaaaca cgcctgacgg caaaacgata gtttcccctg aaaaattccc ggggcgttca 900
tcaacaaatg attctattgt tgtgagcggt gatccgagat ttgccggtac gataaaaatc 960
acaaccagcg cagtcatcga taaccgtgca aacctgaatt atcttctgag ccattccggt 1020
ctggactata aacgcaatat tctgaatgac cggaatccgg tggtgacaga ggatgtggaa 1080
ggtgacaaga aaatttataa tgctgaagtt gctgaatggg ataagttacg gcaaagattg 1140
cttgatgcca gaaataaaat cacctctgct gaatctgcgg taaattcggc gagaaataac 1200
ctcagtgcca gaacaaatga gcaaaagcat gcaaatgacg ctcttaatgc cctgttgaag 1260
gaaaaagaga atatccgtaa ccagctttcc ggcatcaatc agaagatagc ggaagagaaa 1320
agaaaacagg atgaactgaa ggcaacgaaa gacgcaatta atttcacaac agagttcctg 1380
aaatcagttt cagaaaaata tggtgcaaaa gctgagcagt tagccagaga gatggccggg 1440
caggctaaag ggaagaaaat acgtaatgtt gaagaggcat taaaaacgta tgaaaagtac 1500
cgggctgaca ttaacaaaaa aattaatgca aaagatcgtg cagcgattgc cgcagccctt 1560
gagtctgtga agctgtctga tatatcgtct aatctgaaca gattcagtcg gggactggga 1620
tatgcaggaa aatttacaag tcttgctgac tggatcactg agtttggtaa ggctgtccgg 1680
acagagaact ggcgtcctct ttttgttaaa acagaaacca tcatagcagg caatgccgca 1740
acggctcttg tggcactggt cttcagtatt cttaccggaa gcgctttagg cattatcggg 1800
tatggtttac tgatggctgt caccggtgcg ctgattgatg aatcgcttgt ggaaaaagcg 1860
aataagttct ggggtattta a 1881
<210> 58
<211> 626
<212> PRT
<213> 大肠杆菌
<400> 58
Met Ser Asp Pro Val Arg Ile Thr Asn Pro Gly Ala Glu Ser Leu Gly
1 5 10 15
Tyr Asp Ser Asp Gly His Glu Ile Met Ala Val Asp Ile Tyr Val Asn
20 25 30
Pro Pro Arg Val Asp Val Phe His Gly Thr Pro Pro Ala Trp Ser Ser
35 40 45
Phe Gly Asn Lys Thr Ile Trp Gly Gly Asn Glu Trp Val Asp Asp Ser
50 55 60
Pro Thr Arg Ser Asp Ile Glu Lys Arg Asp Lys Glu Ile Thr Ala Tyr
65 70 75 80
Lys Asn Thr Leu Ser Ala Gln Gln Lys Glu Asn Glu Asn Lys Arg Thr
85 90 95
Glu Ala Gly Lys Arg Leu Ser Ala Ala Ile Ala Ala Arg Glu Lys Asp
100 105 110
Glu Asn Thr Leu Lys Thr Leu Arg Ala Gly Asn Ala Asp Ala Ala Asp
115 120 125
Ile Thr Arg Gln Glu Phe Arg Leu Leu Gln Ala Glu Leu Arg Glu Tyr
130 135 140
Gly Phe Arg Thr Glu Ile Ala Gly Tyr Asp Ala Leu Arg Leu His Thr
145 150 155 160
Glu Ser Arg Met Leu Phe Ala Asp Ala Asp Ser Leu Arg Ile Ser Pro
165 170 175
Arg Glu Ala Arg Ser Leu Ile Glu Gln Ala Glu Lys Arg Gln Lys Asp
180 185 190
Ala Gln Asn Ala Asp Lys Lys Ala Ala Asp Met Leu Ala Glu Tyr Glu
195 200 205
Arg Arg Lys Gly Ile Leu Asp Thr Arg Leu Ser Glu Leu Glu Lys Asn
210 215 220
Gly Gly Ala Ala Leu Ala Val Leu Asp Ala Gln Gln Ala Arg Leu Leu
225 230 235 240
Gly Gln Gln Thr Arg Asn Asp Arg Ala Ile Ser Glu Ala Arg Asn Lys
245 250 255
Leu Ser Ser Val Thr Glu Ser Leu Lys Thr Ala Arg Asn Ala Leu Thr
260 265 270
Arg Ala Glu Gln Gln Leu Thr Gln Gln Lys Asn Thr Pro Asp Gly Lys
275 280 285
Thr Ile Val Ser Pro Glu Lys Phe Pro Gly Arg Ser Ser Thr Asn His
290 295 300
Ser Ile Val Val Ser Gly Asp Pro Arg Phe Ala Gly Thr Ile Lys Ile
305 310 315 320
Thr Thr Ser Ala Val Ile Asp Asn Arg Ala Asn Leu Asn Tyr Leu Leu
325 330 335
Thr His Ser Gly Leu Asp Tyr Lys Arg Asn Ile Leu Asn Asp Arg Asn
340 345 350
Pro Val Val Thr Glu Asp Val Glu Gly Asp Lys Lys Ile Tyr Asn Ala
355 360 365
Glu Val Ala Glu Trp Asp Lys Leu Arg Gln Arg Leu Leu Asp Ala Arg
370 375 380
Asn Lys Ile Thr Ser Ala Glu Ser Ala Ile Asn Ser Ala Arg Asn Asn
385 390 395 400
Val Ser Ala Arg Thr Asn Glu Gln Lys His Ala Asn Asp Ala Leu Asn
405 410 415
Ala Leu Leu Lys Glu Lys Glu Asn Ile Arg Ser Gln Leu Ala Asp Ile
420 425 430
Asn Gln Lys Ile Ala Glu Glu Lys Arg Lys Arg Asp Glu Ile Asn Met
435 440 445
Val Lys Asp Ala Ile Lys Leu Thr Ser Asp Phe Tyr Arg Thr Ile Tyr
450 455 460
Asp Glu Phe Gly Lys Gln Ala Ser Glu Leu Ala Lys Glu Leu Ala Ser
465 470 475 480
Val Ser Gln Gly Lys Gln Ile Lys Ser Val Asp Asp Ala Leu Asn Ala
485 490 495
Phe Asp Lys Phe Arg Asn Asn Leu Asn Lys Lys Tyr Asn Ile Gln Asp
500 505 510
Arg Met Ala Ile Ser Lys Ala Leu Glu Ala Ile Asn Gln Val His Met
515 520 525
Ala Glu Asn Phe Lys Leu Phe Ser Lys Ala Phe Gly Phe Thr Gly Lys
530 535 540
Val Ile Glu Arg Tyr Asp Val Ala Val Glu Leu Gln Lys Ala Val Lys
545 550 555 560
Thr Asp Asn Trp Arg Pro Phe Phe Val Lys Leu Glu Ser Leu Ala Ala
565 570 575
Gly Arg Ala Ala Ser Ala Val Thr Ala Trp Ala Phe Ser Val Met Leu
580 585 590
Gly Thr Pro Val Gly Ile Leu Gly Phe Ala Ile Ile Met Ala Ala Val
595 600 605
Ser Ala Leu Val Asn Asp Lys Phe Ile Glu Gln Val Asn Lys Leu Ile
610 615 620
Gly Ile
625
<210> 59
<211> 1881
<212> DNA
<213> 大肠杆菌
<400> 59
atgtctgacc ctgtacgtat tacaaatccc ggtgcagaat cgctgggata tgattcagat 60
ggccatgaaa ttatggccgt tgatatttat gtaaaccctc cacgtgtcga tgtctttcat 120
ggtaccccgc ctgcatggag ttccttcggg aacaaaacca tctggggtgg aaacgagtgg 180
gtcgatgatt ccccaacccg aagtgatatc gaaaaaaggg acaaggaaat cacagcgtac 240
aaaaacacgc tcagcgcgca gcagaaagag aatgagaata agcgtactga agctggaaaa 300
cgcctttctg cggcaattgc tgcaagggaa aaagatgaaa acacactgaa aacactccgt 360
gccggaaacg cagatgccgc tgatattaca cgacaggagt tcagactcct gcaggcagag 420
ctgagagaat acggattccg tactgaaatc gccggatatg atgccctccg gctgcataca 480
gagagccgga tgctgtttgc tgatgctgat tctcttcgta tatctccccg cgaggccagg 540
tcgttaatcg aacaggctga aaaacggcag aaggatgcgc agaacgcaga caagaaggcc 600
gctgatatgc ttgctgaata cgagcgcaga aaaggtattc tggacacgcg gttgtcagag 660
ctggaaaaaa atggcggggc agcccttgcc gttcttgatg cacaacaggc ccgtctgctc 720
gggcagcaga cacggaatga cagggccatt tcagaggccc ggaataaact cagttcggtg 780
acggaatcgc ttaagacggc ccgtaatgca ttaaccagag ctgaacaaca gctgacgcaa 840
cagaaaaaca cgcctgacgg caaaacgata gtttcccctg aaaaattccc ggggcgttca 900
tcaacaaatc attctattgt tgtgagtggt gatccgaggt ttgccggtac gataaaaatc 960
acaaccagcg cggtcatcga taaccgtgca aacctgaatt atcttctgac ccattccggt 1020
ctggactata aacgcaatat tctgaatgac cggaatccgg tggtgacaga ggatgtggaa 1080
ggtgacaaga aaatttataa tgctgaagtt gctgaatggg ataagttacg gcaacgattg 1140
cttgatgcca gaaataaaat cacctctgct gaatctgcga taaattcggc gagaaataac 1200
gtcagtgcca gaacaaatga acaaaagcat gcaaatgacg ctcttaatgc cctgttgaag 1260
gaaaaagaga atatccgtag ccagcttgct gacatcaatc agaaaatagc tgaagagaaa 1320
agaaaaaggg atgaaataaa tatggtaaag gatgccataa aactcacctc tgatttctac 1380
agaacgatat atgatgagtt cggtaaacaa gcatccgaac ttgctaagga gctggcttct 1440
gtatctcaag ggaaacagat taagagtgtg gatgatgcac tgaacgcttt tgataaattc 1500
cgtaataatc tgaacaagaa atataacata caagatcgca tggccatttc taaagccctg 1560
gaagctatta atcaggtcca tatggcggag aattttaagc tgttcagtaa ggcatttggt 1620
tttaccggaa aagttattga acgttatgat gttgctgtgg agttacaaaa ggctgtaaaa 1680
acggacaact ggcgtccatt ttttgtaaaa cttgaatcac tggcagcagg aagagctgct 1740
tcagcagtta cagcatgggc gttttccgtc atgctgggaa cccctgtagg tattctgggt 1800
tttgcaatta ttatggcggc tgtgagtgcg cttgttaatg ataagtttat tgagcaggtc 1860
aataaactta ttggtatctg a 1881
<210> 60
<211> 271
<212> PRT
<213> 大肠杆菌
<400> 60
Met Glu Thr Leu Thr Val His Ala Pro Ser Pro Ser Thr Asn Leu Pro
1 5 10 15
Ser Tyr Gly Asn Gly Ala Phe Ser Leu Ser Ala Pro His Val Pro Gly
20 25 30
Ala Gly Pro Leu Leu Val Gln Val Val Tyr Ser Phe Phe Gln Ser Pro
35 40 45
Asn Met Cys Leu Gln Ala Leu Thr Gln Leu Glu Asp Tyr Ile Lys Lys
50 55 60
His Gly Ala Ser Asn Pro Leu Thr Leu Gln Ile Ile Ser Thr Asn Ile
65 70 75 80
Gly Tyr Phe Cys Asn Ala Asp Arg Asn Leu Val Leu His Pro Gly Ile
85 90 95
Ser Val Tyr Asp Ala Tyr His Phe Ala Lys Pro Ala Pro Ser Gln Tyr
100 105 110
Asp Tyr Arg Ser Met Asn Met Lys Gln Met Ser Gly Asn Val Thr Thr
115 120 125
Pro Ile Val Ala Leu Ala His Tyr Leu Trp Gly Asn Gly Ala Glu Arg
130 135 140
Ser Val Asn Ile Ala Asn Ile Gly Leu Lys Ile Ser Pro Met Lys Ile
145 150 155 160
Asn Gln Ile Lys Asp Ile Ile Lys Ser Gly Val Val Gly Thr Phe Pro
165 170 175
Val Ser Thr Lys Phe Thr His Ala Thr Gly Asp Tyr Asn Val Ile Thr
180 185 190
Gly Ala Tyr Leu Gly Asn Ile Thr Leu Lys Thr Glu Gly Thr Leu Thr
195 200 205
Ile Ser Ala Asn Gly Ser Trp Thr Tyr Asn Gly Val Val Arg Ser Tyr
210 215 220
Asp Asp Lys Tyr Asp Phe Asn Ala Ser Thr His Arg Gly Ile Ile Gly
225 230 235 240
Glu Ser Leu Thr Arg Leu Gly Ala Met Phe Ser Gly Lys Glu Tyr Gln
245 250 255
Ile Leu Leu Pro Gly Glu Ile His Ile Lys Glu Ser Gly Lys Arg
260 265 270
<210> 61
<211> 816
<212> DNA
<213> 大肠杆菌
<400> 61
atggaaacct taactgttca tgcaccatca ccatcaacta acttaccaag ttatggcaat 60
ggtgcatttt ctctttcagc accacatgtg cctggtgctg gccctctttt agtccaggtt 120
gtttatagtt ttttccagag tccaaacatg tgtcttcagg ctttaactca acttgaggat 180
tacatcaaaa aacatggggc cagcaaccct ctcacattgc agatcatatc gacaaatatt 240
ggttacttct gtaacgccga ccgaaatctg gttcttcacc ctggaataag cgtttatgac 300
gcttaccact tcgcaaaacc agcgccaagt caatatgact atcgctcaat gaatatgaaa 360
caaatgagcg gtaatgtcac tacaccaatt gtggcgcttg ctcactattt atggggtaat 420
ggcgctgaaa ggagcgttaa tatcgccaac attggtctta aaatttcccc tatgaaaatt 480
aatcagataa aagacattat aaaatctggt gtagtaggca cattccctgt ttctacaaag 540
ttcacacatg ccactggtga ttataatgtt attaccggtg catatcttgg taatatcaca 600
ctgaaaacag aaggtacttt aactatctct gccaatggct cctggactta caatggcgtt 660
gttcgttcat atgatgataa atacgatttt aacgccagca ctcaccgtgg cattatcgga 720
gagtcgctca caaggctcgg ggcgatgttt tctggtaaag agtaccagat actgcttcct 780
ggtgaaattc acattaaaga aagtggtaag cgataa 816
<210> 62
<211> 387
<212> PRT
<213> 大肠杆菌
<400> 62
Met Gly Ser Asn Gly Ala Asp Asn Ala His Asn Asn Ala Phe Gly Gly
1 5 10 15
Gly Lys Asn Pro Gly Ile Gly Asn Thr Ser Gly Ala Gly Ser Asn Gly
20 25 30
Ser Ala Ser Ser Asn Arg Gly Asn Ser Asn Gly Trp Ser Trp Ser Asn
35 40 45
Lys Pro His Lys Asn Asp Gly Phe His Ser Asp Gly Ser Tyr His Ile
50 55 60
Thr Phe His Gly Asp Asn Asn Ser Lys Pro Lys Pro Gly Gly Asn Ser
65 70 75 80
Gly Asn Arg Gly Asn Asn Gly Asp Gly Ala Ser Ala Lys Val Gly Glu
85 90 95
Ile Thr Ile Thr Pro Asp Asn Ser Lys Pro Gly Arg Tyr Ile Ser Ser
100 105 110
Asn Pro Glu Tyr Ser Leu Leu Ala Lys Leu Ile Asp Ala Glu Ser Ile
115 120 125
Lys Gly Thr Glu Val Tyr Thr Phe His Thr Arg Lys Gly Gln Tyr Val
130 135 140
Lys Val Thr Val Pro Asp Ser Asn Ile Asp Lys Met Arg Val Asp Tyr
145 150 155 160
Val Asn Trp Lys Gly Pro Lys Tyr Asn Asn Lys Leu Val Lys Arg Phe
165 170 175
Val Ser Gln Phe Leu Leu Phe Arg Lys Glu Glu Lys Glu Lys Asn Glu
180 185 190
Lys Glu Ala Leu Leu Lys Ala Ser Glu Leu Val Ser Gly Met Gly Asp
195 200 205
Lys Leu Gly Glu Tyr Leu Gly Val Lys Tyr Lys Asn Val Ala Lys Glu
210 215 220
Val Ala Asn Asp Ile Lys Asn Phe His Gly Arg Asn Ile Arg Ser Tyr
225 230 235 240
Asn Glu Ala Met Ala Ser Leu Asn Lys Val Leu Ala Asn Pro Lys Met
245 250 255
Lys Val Asn Lys Ser Asp Lys Asp Ala Ile Val Asn Ala Trp Lys Gln
260 265 270
Val Asn Ala Lys Asp Met Ala Asn Lys Ile Gly Asn Leu Gly Lys Ala
275 280 285
Phe Lys Val Ala Asp Leu Ala Ile Lys Val Glu Lys Ile Arg Glu Lys
290 295 300
Ser Ile Glu Gly Tyr Asn Thr Gly Asn Trp Gly Pro Leu Leu Leu Glu
305 310 315 320
Val Glu Ser Trp Ile Ile Gly Gly Val Val Ala Gly Val Ala Ile Ser
325 330 335
Leu Phe Gly Ala Val Leu Ser Phe Leu Pro Ile Ser Gly Leu Ala Val
340 345 350
Thr Ala Leu Gly Val Ile Gly Ile Met Thr Ile Ser Tyr Leu Ser Ser
355 360 365
Phe Ile Asp Ala Asn Arg Val Ser Asn Ile Asn Asn Ile Ile Ser Ser
370 375 380
Val Ile Arg
385
<210> 63
<211> 1171
<212> DNA
<213> 大肠杆菌
<400> 63
atgggtagta atggcgcaga taatgcacat aacaatgctt ttggtggagg gaaaaatccg 60
ggcattggta ataccagtgg cgcaggaagt aatggtagtg catcaagtaa ccgaggaaat 120
tccaatggat ggtcatggag taataagcct cataaaaatg atggcttcca cagtgatggt 180
tcttaccata ttacatttca tggggacaat aattcaaagc ctaaacctgg agggaatagt 240
ggaaatcgag gtaataacgg tgatggagcg agtgctaagg ttggagagat aacaatcaca 300
cctgacaact cgaaaccagg tcgttatatt tcgtcaaatc ctgaatattc attgttggca 360
aaattaattg atgcggaatc aattaaaggt acagaggtat atacttttca caccagaaaa 420
ggtcagtatg ttaaggttac tgttccagat agtaatattg ataaaatgag agttgattat 480
gtgaactgga agggaccgaa atataacaat aaacttgtga agaggtttgt gagccagttt 540
ttattattta ggaaggaaga aaaagaaaaa aatgaaaaag aagccttgct aaaggctagt 600
gaacttgtgt ctggtatggg ggataagctt ggcgagtatc ttggagtaaa atataaaaat 660
gtagctaagg aagttgccaa tgatattaaa aacttccatg gtcgtaatat tcgtagctat 720
aatgaagcaa tggcttcact taataaagtg ttagcaaatc caaagatgaa agtaaacaaa 780
tctgataagg atgccattgt gaatgcctgg aaacaggtta atgctaagga catggctaat 840
aagattggta atcttggcaa ggcatttaag gttgctgatt tagctataaa ggttgagaaa 900
attagggaaa aaagcattga gggatacaat actggcaact ggggacctct cctgttggag 960
gttgaatcat ggatcattgg tggcgttgtt gctggagttg ctattagttt attcggggct 1020
gtgttgagtt ttctcccaat ctctggactt gcagttactg cgttgggggt aataggaata 1080
atgacgatta gttacttgtc atctttcata gatgcaaatc gagtttcgaa tataaataac 1140
attatatcta gtgttattcg agcaaatcga g 1171
<210> 64
<211> 103
<212> PRT
<213> 大肠杆菌
<400> 64
Met Arg Thr Leu Thr Leu Asn Glu Leu Asp Ser Val Ser Gly Gly Ala
1 5 10 15
Ser Gly Arg Asp Ile Ala Met Ala Ile Gly Thr Leu Ser Gly Gln Phe
20 25 30
Val Ala Gly Gly Ile Gly Ala Ala Ala Gly Gly Val Ala Gly Gly Ala
35 40 45
Ile Tyr Asp Tyr Ala Ser Thr His Lys Pro Asn Pro Ala Met Ser Pro
50 55 60
Ser Gly Leu Gly Gly Thr Ile Lys Gln Lys Pro Glu Gly Ile Pro Ser
65 70 75 80
Glu Ala Trp Asn Tyr Ala Ala Gly Arg Leu Cys Asn Trp Ser Pro Asn
85 90 95
Asn Leu Ser Asp Val Cys Leu
100
<210> 65
<211> 312
<212> DNA
<213> 大肠杆菌
<400> 65
atgagaactc tgactctaaa tgaattagat tctgtttctg gtggtgcttc agggcgtgat 60
attgcgatgg ctataggaac actatccgga caatttgttg caggaggaat tggagcagct 120
gctgggggtg tggctggagg tgcaatatat gactatgcat ccactcacaa acctaatcct 180
gcaatgtctc catccggttt aggaggaaca attaagcaaa aacccgaagg gataccttca 240
gaagcatgga actatgctgc gggaagattg tgtaattgga gtccaaataa tcttagtgat 300
gtttgtttat aa 312
<210> 66
<211> 58
<212> PRT
<213> 哥伦比亚肠球菌
<400> 66
Met Met Asn Ala Thr Glu Asn Gln Ile Phe Val Glu Thr Val Ser Asp
1 5 10 15
Gln Glu Leu Glu Met Leu Ile Gly Gly Ala Gly Arg Gly Trp Ile Lys
20 25 30
Thr Leu Thr Lys Asp Cys Pro Asn Val Ile Ser Ser Ile Cys Ala Gly
35 40 45
Thr Ile Ile Thr Ala Cys Lys Asn Cys Ala
50 55
<210> 67
<211> 177
<212> DNA
<213> 哥伦比亚肠球菌
<400> 67
atgatgaatg ctactgaaaa ccaaattttt gttgagactg tgagtgacca agaattagaa 60
atgttaattg gtggtgcagg tcgtggatgg attaagactt taacaaaaga ttgtccaaat 120
gtgatttctt caatttgtgc aggtacaatt attacagctt gtaaaaattg tgcttaa 177
<210> 68
<211> 59
<212> PRT
<213> 弯曲乳杆菌
<400> 68
Met Asn Asn Val Lys Glu Leu Ser Met Thr Glu Leu Gln Thr Ile Thr
1 5 10 15
Gly Gly Ala Arg Ser Tyr Gly Asn Gly Val Tyr Cys Asn Asn Lys Lys
20 25 30
Cys Trp Val Asn Arg Gly Glu Ala Thr Gln Ser Ile Ile Gly Gly Met
35 40 45
Ile Ser Gly Trp Ala Ser Gly Leu Ala Gly Met
50 55
<210> 69
<211> 180
<212> DNA
<213> 弯曲乳杆菌
<400> 69
atgaataatg taaaagaatt aagtatgaca gaattacaaa caattaccgg cggtgctaga 60
tcatatggca acggtgttta ctgtaataat aaaaaatgtt gggtaaatcg gggtgaagca 120
acgcaaagta ttattggtgg tatgattagc ggctgggcta gtggtttagc tggaatgtaa 180
<210> 70
<211> 64
<212> PRT
<213> 链霉菌属
<400> 70
Met Arg Ser Glu Met Thr Leu Thr Ser Thr Asn Ser Ala Glu Ala Leu
1 5 10 15
Ala Ala Gln Asp Phe Ala Asn Thr Val Leu Ser Ala Ala Ala Pro Gly
20 25 30
Phe His Ala Asp Cys Glu Thr Pro Ala Met Ala Thr Pro Ala Thr Pro
35 40 45
Thr Val Ala Gln Phe Val Ile Gln Gly Ser Thr Ile Cys Leu Val Cys
50 55 60
<210> 71
<211> 195
<212> DNA
<213> 链霉菌属
<400> 71
gtgcgatctg agatgactct tacgagcacg aattccgctg aggctctggc ggcgcaggac 60
tttgcgaaca ccgttctcag cgcggcggcc ccgggcttcc acgcggactg cgagacgccg 120
gccatggcca ccccggccac gccgaccgtc gcccagttcg tgatccaggg cagcacgatc 180
tgcctggtct gctga 195
<210> 72
<211> 65
<212> PRT
<213> 嗜碱芽孢杆菌
<400> 72
Met Val Asn Ser Lys Asp Leu Arg Asn Pro Glu Phe Arg Lys Ala Gln
1 5 10 15
Gly Leu Gln Phe Val Asp Glu Val Asn Glu Lys Glu Leu Ser Ser Leu
20 25 30
Ala Gly Ser Gly Asp Val His Ala Gln Thr Thr Trp Pro Cys Ala Thr
35 40 45
Val Gly Val Ser Val Ala Leu Cys Pro Thr Thr Lys Cys Thr Ser Gln
50 55 60
Cys
65
<210> 73
<211> 198
<212> DNA
<213> 嗜碱芽孢杆菌
<400> 73
atggtaaatt caaaagattt gcgtaatcct gaattccgca aagcccaagg tctacaattc 60
gttgacgagg tgaacgagaa ggaactttcg tctctagctg gttcaggaga tgtgcatgca 120
caaacaactt ggccttgcgc tacagttggt gtctccgtag ccttgtgccc aactacaaag 180
tgtacaagcc agtgctaa 198
<210> 74
<211> 66
<212> PRT
<213> 分歧食肉杆菌
<400> 74
Met Lys Asn Leu Lys Glu Gly Ser Tyr Thr Ala Val Asn Thr Asp Glu
1 5 10 15
Leu Lys Ser Ile Asn Gly Gly Thr Lys Tyr Tyr Gly Asn Gly Val Tyr
20 25 30
Cys Asn Ser Lys Lys Cys Trp Val Asp Trp Gly Gln Ala Ser Gly Cys
35 40 45
Ile Gly Gln Thr Val Val Gly Gly Trp Leu Gly Gly Ala Ile Pro Gly
50 55 60
Lys Cys
65
<210> 75
<211> 201
<212> DNA
<213> 分歧食肉杆菌
<400> 75
atgaaaaact taaaagaagg ttcatacact gctgttaata ctgatgaatt aaaaagtatc 60
aatggtggaa caaaatatta tgggaatggc gtttattgca attctaaaaa atgttgggta 120
gattggggac aagcttcagg ttgtatcggt caaactgttg ttggcggatg gctaggcgga 180
gctataccag gtaaatgcta a 201
<210> 76
<211> 63
<212> PRT
<213> 分歧食肉杆菌
<400> 76
Met Ile Lys Arg Glu Lys Asn Arg Thr Ile Ser Ser Leu Gly Tyr Glu
1 5 10 15
Glu Ile Ser Asn His Lys Leu Gln Glu Ile Gln Gly Gly Lys Gly Ile
20 25 30
Leu Gly Lys Leu Gly Val Val Gln Ala Gly Val Asp Phe Val Ser Gly
35 40 45
Val Trp Ala Gly Ile Lys Gln Ser Ala Lys Asp His Pro Asn Ala
50 55 60
<210> 77
<211> 192
<212> DNA
<213> 分歧食肉杆菌
<400> 77
atgattaaaa gagaaaagaa cagaacaatt tcttcccttg gttatgaaga aatttctaat 60
cataaattgc aagaaataca aggtggaaaa ggaattcttg gtaaactagg agtagtacag 120
gcaggagtgg attttgtatc aggagtgtgg gctggaataa aacagtctgc caaagatcat 180
cctaatgcgt aa 192
<210> 78
<211> 75
<212> PRT
<213> 分歧食肉杆菌
<400> 78
Met Lys Lys Gln Ile Leu Lys Gly Leu Val Ile Val Val Cys Leu Ser
1 5 10 15
Gly Ala Thr Phe Phe Ser Thr Pro Gln Gln Ala Ser Ala Ala Ala Pro
20 25 30
Lys Ile Thr Gln Lys Gln Lys Asn Cys Val Asn Gly Gln Leu Gly Gly
35 40 45
Met Leu Ala Gly Ala Leu Gly Gly Pro Gly Gly Val Val Leu Gly Gly
50 55 60
Ile Gly Gly Ala Ile Ala Gly Gly Cys Phe Asn
65 70 75
<210> 79
<211> 228
<212> DNA
<213> 分歧食肉杆菌
<400> 79
atgaaaaaac aaattttaaa agggttggtt atagttgttt gtttatctgg ggcaacattt 60
ttctcaacac cacaacaagc ttctgctgct gcaccgaaaa ttactcaaaa acaaaaaaat 120
tgtgttaatg gacaattagg tggaatgctt gctggagctt tgggtggacc tggcggagtt 180
gtgttaggtg gtataggtgg tgcaatagca ggaggttgtt ttaattaa 228
<210> 80
<211> 72
<212> PRT
<213> 耐久肠球菌
<400> 80
Met Gln Thr Ile Lys Glu Leu Asn Thr Met Glu Leu Gln Glu Ile Ile
1 5 10 15
Gly Gly Glu Asn Asp His Arg Met Pro Tyr Glu Leu Asn Arg Pro Asn
20 25 30
Asn Leu Ser Lys Gly Gly Ala Lys Cys Ala Ala Gly Ile Leu Gly Ala
35 40 45
Gly Leu Gly Ala Val Gly Gly Gly Pro Gly Gly Phe Ile Ser Ala Gly
50 55 60
Ile Ser Ala Val Leu Gly Cys Met
65 70
<210> 81
<211> 219
<212> DNA
<213> 耐久肠球菌
<400> 81
atgcaaacga tcaaagaatt gaacacgatg gaattacaag aaataattgg aggtgaaaat 60
gaccatcgga tgccttacga attgaaccgt ccaaataatt tatccaaagg tggggctaag 120
tgtgctgctg gaatacttgg cgctggacta ggcgcagtag gcggtggacc tggcggattt 180
attagtgccg gaatcagtgc tgttcttggt tgtatgtaa 219
<210> 82
<211> 72
<212> PRT
<213> 耐久肠球菌
<400> 82
Met Gln Thr Ile Lys Glu Leu Asn Thr Met Glu Leu Gln Lys Ile Ile
1 5 10 15
Gly Gly Glu Asn Asp His Arg Met Pro Tyr Glu Leu Asn Arg Pro Asn
20 25 30
Asn Leu Ser Lys Gly Gly Ala Lys Cys Ala Ala Gly Ile Leu Gly Ala
35 40 45
Gly Leu Gly Ala Val Gly Gly Gly Pro Gly Gly Phe Ile Ser Ala Gly
50 55 60
Ile Ser Ala Val Leu Gly Cys Met
65 70
<210> 83
<211> 219
<212> DNA
<213> 耐久肠球菌
<400> 83
atgcaaacga tcaaagaatt gaacacgatg gaattacaaa aaataattgg aggtgaaaat 60
gaccatcgga tgccttacga attgaaccgt ccaaataatt tatccaaagg tggagctaag 120
tgcgctgccg gaatacttgg tgctggatta ggcgcagtag gcggtggacc tggcggattt 180
attagtgccg gaatcagtgc tgttcttggt tgtatgtaa 219
<210> 84
<211> 220
<212> PRT
<213> 似马停乳链球菌亚种
<400> 84
Met Lys Lys Leu Lys Arg Leu Val Ile Ser Leu Val Thr Ser Leu Leu
1 5 10 15
Val Ile Ser Ser Thr Val Pro Ala Leu Val Tyr Ala Asn Glu Thr Asn
20 25 30
Asn Phe Ala Glu Thr Gln Lys Glu Ile Thr Thr Asn Ser Glu Ala Thr
35 40 45
Leu Thr Asn Glu Asp Tyr Thr Lys Leu Thr Ser Glu Val Lys Thr Ile
50 55 60
Tyr Thr Asn Leu Ile Gln Tyr Asp Gln Thr Lys Asn Lys Phe Tyr Val
65 70 75 80
Asp Glu Asp Lys Thr Glu Gln Tyr Tyr Asn Tyr Asp Asp Glu Ser Ile
85 90 95
Lys Gly Val Tyr Leu Met Lys Asp Ser Leu Asn Asp Glu Leu Asn Asn
100 105 110
Asn Asn Ser Ser Asn Tyr Ser Glu Ile Ile Asn Gln Lys Ile Ser Glu
115 120 125
Ile Asp Tyr Val Leu Gln Gly Asn Asp Ile Asn Asn Leu Ile Pro Ser
130 135 140
Asn Thr Arg Val Lys Arg Ser Ala Asp Phe Ser Trp Ile Gln Arg Cys
145 150 155 160
Leu Glu Glu Ala Trp Gly Tyr Ala Ile Ser Leu Val Thr Leu Lys Gly
165 170 175
Ile Ile Asn Leu Phe Lys Ala Gly Lys Phe Glu Ala Ala Ala Ala Lys
180 185 190
Leu Ala Ser Ala Thr Ala Gly Arg Ile Ala Gly Met Ala Ala Leu Phe
195 200 205
Ala Phe Val Ala Thr Cys Gly Ala Thr Thr Val Ser
210 215 220
<210> 85
<211> 663
<212> DNA
<213> 似马停乳链球菌亚种
<400> 85
atgaaaaaat taaaacgtct tgttatctct cttgttactt cattactagt aatttcaagt 60
acagttccag cacttgttta cgctaatgaa acaaataact ttgcagaaac tcaaaaagaa 120
attacaacaa attcagaagc aacattaacc aatgaagact acactaaatt aacttccgaa 180
gtaaaaacaa tttatacaaa tctgattcaa tacgaccaaa caaaaaacaa attttacgtc 240
gatgaagaca aaactgaaca atattataac tacgatgatg aaagtataaa aggggtttat 300
ctcatgaaag atagtttgaa cgatgagtta aacaataata actcttcaaa ctattctgaa 360
ataattaatc aaaaaatctc tgaaattgac tatgtccttc aaggaaacga tataaataat 420
ttaattccta gcaataccag agtaaaaaga tcagcagatt tttcttggat tcaaagatgt 480
ctagaagaag catggggata tgctattagt ctagttactc taaaaggaat aatcaatcta 540
tttaaagcag gaaaatttga agctgctgct gctaaattag cttctgctac agcaggtaga 600
atcgctggaa tggctgcctt atttgctttc gtagcaactt gcggtgcgac aactgtatca 660
taa 663
<210> 86
<211> 57
<212> PRT
<213> 粪肠球菌
<400> 86
Met Lys Gln Tyr Lys Val Leu Asn Glu Lys Glu Met Lys Lys Pro Ile
1 5 10 15
Gly Gly Glu Ser Val Phe Ser Lys Ile Gly Asn Ala Val Gly Pro Ala
20 25 30
Ala Tyr Trp Ile Leu Lys Gly Leu Gly Asn Met Ser Asp Val Asn Gln
35 40 45
Ala Asp Arg Ile Asn Arg Lys Lys His
50 55
<210> 87
<211> 174
<212> DNA
<213> 粪肠球菌
<400> 87
atgaagcaat ataaagtatt gaatgaaaaa gaaatgaaaa aacctattgg gggagagtcg 60
gtttttagta aaataggtaa tgctgtaggt ccagctgctt attggatttt aaaaggatta 120
ggtaatatga gtgatgtaaa ccaagctgat agaattaata gaaagaaaca ttaa 174
<210> 88
<211> 44
<212> PRT
<213> 粪肠球菌
<400> 88
Met Gly Ala Ile Ala Lys Leu Val Ala Lys Phe Gly Trp Pro Ile Val
1 5 10 15
Lys Lys Tyr Tyr Lys Gln Ile Met Gln Phe Ile Gly Glu Gly Trp Ala
20 25 30
Ile Asn Lys Ile Ile Asp Trp Ile Lys Lys His Ile
35 40
<210> 89
<211> 135
<212> DNA
<213> 粪肠球菌
<400> 89
atgggagcaa tcgcaaaatt agtagcaaag tttggatggc caattgttaa aaagtattac 60
aaacaaatta tgcaatttat tggagaagga tgggcaatta acaaaattat tgattggatc 120
aaaaaacata tttaa 135
<210> 90
<211> 43
<212> PRT
<213> 粪肠球菌
<400> 90
Met Gly Ala Ile Ala Lys Leu Val Ala Lys Phe Gly Trp Pro Phe Ile
1 5 10 15
Lys Lys Phe Tyr Lys Gln Ile Met Gln Phe Ile Gly Gln Gly Trp Thr
20 25 30
Ile Asp Gln Ile Glu Lys Trp Leu Lys Arg His
35 40
<210> 91
<211> 132
<212> DNA
<213> 粪肠球菌
<400> 91
atgggagcaa tcgcaaaatt agtagcaaag tttggatggc catttattaa aaaattctac 60
aaacaaatta tgcagtttat cggacaagga tggacaatag atcaaattga aaaatggtta 120
aaaagacatt ga 132
<210> 92
<211> 74
<212> PRT
<213> 粪肠球菌
<400> 92
Met Leu Asn Lys Lys Leu Leu Glu Asn Gly Val Val Asn Ala Val Thr
1 5 10 15
Ile Asp Glu Leu Asp Ala Gln Phe Gly Gly Met Ser Lys Arg Asp Cys
20 25 30
Asn Leu Met Lys Ala Cys Cys Ala Gly Gln Ala Val Thr Tyr Ala Ile
35 40 45
His Ser Leu Leu Asn Arg Leu Gly Gly Asp Ser Ser Asp Pro Ala Gly
50 55 60
Cys Asn Asp Ile Val Arg Lys Tyr Cys Lys
65 70
<210> 93
<211> 225
<212> DNA
<213> 粪肠球菌
<400> 93
atgttaaata aaaaattatt agaaaatggt gtagtaaatg ctgtaacaat tgatgaactt 60
gatgctcaat ttggtggaat gagcaaacgt gattgtaact tgatgaaggc gtgttgtgct 120
ggacaagcag taacatatgc tattcatagt cttttaaatc gattaggtgg agactctagt 180
gatccagctg gttgtaatga tattgtaaga aaatattgta aataa 225
<210> 94
<211> 65
<212> PRT
<213> 屎肠球菌
<400> 94
Met Lys His Leu Lys Ile Leu Ser Ile Lys Glu Thr Gln Leu Ile Tyr
1 5 10 15
Gly Gly Thr Thr His Ser Gly Lys Tyr Tyr Gly Asn Gly Val Tyr Cys
20 25 30
Thr Lys Asn Lys Cys Thr Val Asp Trp Ala Lys Ala Thr Thr Cys Ile
35 40 45
Ala Gly Met Ser Ile Gly Gly Phe Leu Gly Gly Ala Ile Pro Gly Lys
50 55 60
Cys
65
<210> 95
<211> 195
<212> DNA
<213> 屎肠球菌
<400> 95
atgaaacatt taaaaatttt gtctattaaa gagacacaac ttatctatgg gggtaccact 60
catagtggaa aatattatgg aaatggagtg tattgcacta aaaataaatg tacggtcgat 120
tgggccaagg caactacttg tattgcagga atgtctatag gtggtttttt aggtggagca 180
attccaggga agtgc 195
<210> 96
<211> 105
<212> PRT
<213> 粪肠球菌
<400> 96
Met Val Lys Glu Asn Lys Phe Ser Lys Ile Phe Ile Leu Met Ala Leu
1 5 10 15
Ser Phe Leu Gly Leu Ala Leu Phe Ser Ala Ser Leu Gln Phe Leu Pro
20 25 30
Ile Ala His Met Ala Lys Glu Phe Gly Ile Pro Ala Ala Val Ala Gly
35 40 45
Thr Val Leu Asn Val Val Glu Ala Gly Gly Trp Val Thr Thr Ile Val
50 55 60
Ser Ile Leu Thr Ala Val Gly Ser Gly Gly Leu Ser Leu Leu Ala Ala
65 70 75 80
Ala Gly Arg Glu Ser Ile Lys Ala Tyr Leu Lys Lys Glu Ile Lys Lys
85 90 95
Lys Gly Lys Arg Ala Val Ile Ala Trp
100 105
<210> 97
<211> 318
<212> DNA
<213> 粪肠球菌
<400> 97
atggttaaag aaaataaatt ttctaagatt tttattttaa tggctttgag ttttttgggg 60
ttagccttgt ttagtgcaag tcttcagttt ttgcccattg cacatatggc taaagagttc 120
ggtataccag cagcagttgc aggaactgtg cttaatgtag ttgaagctgg tggatgggtc 180
actactattg tatcaattct tactgctgta ggtagcggag gtctttcttt actcgctgca 240
gcaggaagag agtcaattaa agcatacctt aagaaagaaa ttaagaaaaa aggaaaaaga 300
gcagttattg cttggtaa 318
<210> 98
<211> 71
<212> PRT
<213> 屎肠球菌
<400> 98
Met Gln Asn Val Lys Glu Leu Ser Thr Lys Glu Met Lys Gln Ile Ile
1 5 10 15
Gly Gly Glu Asn Asp His Arg Met Pro Asn Glu Leu Asn Arg Pro Asn
20 25 30
Asn Leu Ser Lys Gly Gly Ala Lys Cys Gly Ala Ala Ile Ala Gly Gly
35 40 45
Leu Phe Gly Ile Pro Lys Gly Pro Leu Ala Trp Ala Ala Gly Leu Ala
50 55 60
Asn Val Tyr Ser Lys Cys Asn
65 70
<210> 99
<211> 216
<212> DNA
<213> 屎肠球菌
<400> 99
atgcaaaatg taaaagaatt aagtacgaaa gagatgaaac aaattatcgg tggagaaaat 60
gatcacagaa tgcctaatga gttaaataga cctaacaact tatctaaagg tggagcaaaa 120
tgtggtgctg caattgctgg gggattattt ggaatcccaa aaggaccact agcatgggct 180
gctgggttag caaatgtata ctctaaatgc aactaa 216
<210> 100
<211> 58
<212> PRT
<213> 蒙氏肠球菌
<400> 100
Met Lys Lys Leu Thr Ser Lys Glu Met Ala Gln Val Val Gly Gly Lys
1 5 10 15
Tyr Tyr Gly Asn Gly Val Ser Cys Asn Lys Lys Gly Cys Ser Val Asp
20 25 30
Trp Gly Lys Ala Ile Gly Ile Ile Gly Asn Asn Ser Ala Ala Asn Leu
35 40 45
Ala Thr Gly Gly Ala Ala Gly Trp Lys Ser
50 55
<210> 101
<211> 177
<212> DNA
<213> 蒙氏肠球菌
<400> 101
ttgaagaaat taacatcaaa agaaatggca caagtagtag gtggaaaata ctacggtaat 60
ggagtctcat gtaataaaaa agggtgcagt gttgattggg gaaaagctat tggcattatt 120
ggaaataatt ctgctgcgaa tttagctact ggtggagcag ctggttggaa aagttaa 177
<210> 102
<211> 44
<212> PRT
<213> 粪肠球菌
<400> 102
Met Leu Ala Lys Ile Lys Ala Met Ile Lys Lys Phe Pro Asn Pro Tyr
1 5 10 15
Thr Leu Ala Ala Lys Leu Thr Thr Tyr Glu Ile Asn Trp Tyr Lys Gln
20 25 30
Gln Tyr Gly Arg Tyr Pro Trp Glu Arg Pro Val Ala
35 40
<210> 103
<211> 135
<212> DNA
<213> 粪肠球菌
<400> 103
atgttagcaa aaattaaagc gatgattaag aagtttccga acccttatac tttagcagct 60
aagctaacga cttacgaaat taattggtat aaacaacaat acggtcgtta tccttgggag 120
cgccctgtag cataa 135
<210> 104
<211> 71
<212> PRT
<213> 屎肠球菌
<400> 104
Met Arg Lys Lys Leu Phe Ser Leu Ala Leu Ile Gly Ile Phe Gly Leu
1 5 10 15
Val Val Thr Asn Phe Gly Thr Lys Val Asp Ala Ala Thr Arg Ser Tyr
20 25 30
Gly Asn Gly Val Tyr Cys Asn Asn Ser Lys Cys Trp Val Asn Trp Gly
35 40 45
Glu Ala Lys Glu Asn Ile Ala Gly Ile Val Ile Ser Gly Trp Ala Ser
50 55 60
Gly Leu Ala Gly Met Gly His
65 70
<210> 105
<211> 216
<212> DNA
<213> 屎肠球菌
<400> 105
atgagaaaaa aattatttag tttagctctt attggaatat ttgggttagt tgtgacaaat 60
tttggtacaa aagttgatgc agctacgcgt tcatatggta atggtgttta ttgtaataat 120
agtaaatgct gggttaactg gggagaagct aaagagaata ttgcaggaat cgttattagt 180
ggctgggctt ctggtttggc aggtatggga cattaa 216
<210> 106
<211> 34
<212> PRT
<213> 屎肠球菌
<400> 106
Met Asn Phe Leu Lys Asn Gly Ile Ala Lys Trp Met Thr Gly Ala Glu
1 5 10 15
Leu Gln Ala Tyr Lys Lys Lys Tyr Gly Cys Leu Pro Trp Glu Lys Ile
20 25 30
Ser Cys
<210> 107
<211> 105
<212> DNA
<213> 屎肠球菌
<400> 107
atgaattttc ttaaaaatgg tatcgcaaaa tggatgaccg gtgctgaatt gcaagcgtat 60
aaaaagaaat atggatgctt gccatgggaa aaaatttctt gttaa 105
<210> 108
<211> 76
<212> PRT
<213> 粪肠球菌
<400> 108
Met Lys Lys Lys Leu Val Lys Gly Leu Val Ile Cys Gly Met Ile Gly
1 5 10 15
Ile Gly Phe Thr Ala Leu Gly Thr Asn Val Glu Ala Ala Thr Tyr Tyr
20 25 30
Gly Asn Gly Val Tyr Cys Asn Lys Gln Lys Cys Trp Val Asp Trp Ser
35 40 45
Arg Ala Arg Ser Glu Ile Ile Asp Arg Gly Val Lys Ala Tyr Val Asn
50 55 60
Gly Phe Thr Lys Val Leu Gly Gly Ile Gly Gly Arg
65 70 75
<210> 109
<211> 231
<212> DNA
<213> 粪肠球菌
<400> 109
atgaaaaaga aattagttaa aggcttagtt atttgtggca tgattgggat tggttttaca 60
gcattaggaa caaatgtaga agccgccacg tattacggaa atggtgtcta ttgcaataag 120
caaaaatgtt gggtagattg gagtagagca cgttctgaaa ttatagacag aggcgtaaaa 180
gcatacgtca atggatttac gaaagtgtta ggtggtatag gtggaagata a 231
<210> 110
<211> 60
<212> PRT
<213> 粪肠球菌
<400> 110
Met Lys Lys Glu Glu Leu Val Gly Met Ala Lys Glu Asp Phe Leu Asn
1 5 10 15
Val Ile Cys Glu Asn Asp Asn Lys Leu Glu Asn Ser Gly Ala Lys Cys
20 25 30
Pro Trp Trp Asn Leu Ser Cys His Leu Gly Asn Asp Gly Lys Ile Cys
35 40 45
Thr Tyr Ser His Glu Cys Thr Ala Gly Cys Asn Ala
50 55 60
<210> 111
<211> 183
<212> DNA
<213> 粪肠球菌
<400> 111
atgaaaaaag aagaattagt aggaatggct aaggaagact ttttaaatgt tatttgtgaa 60
aatgacaaca aactagaaaa tagtggagca aaatgtcctt ggtggaatct ttcttgtcat 120
ttaggcaatg atggtaaaat ttgcacttat tcacatgaat gtaccgcagg ttgtaatgca 180
taa 183
<210> 112
<211> 61
<212> PRT
<213> 粪肠球菌
<400> 112
Met Thr Glu Leu Asn Lys Arg Leu Gln Leu Lys Arg Asp Val Ser Thr
1 5 10 15
Glu Asn Ser Leu Lys Lys Ile Ser Asn Thr Asp Glu Thr His Gly Gly
20 25 30
Val Thr Thr Ser Ile Pro Cys Thr Val Met Val Ser Ala Ala Val Cys
35 40 45
Pro Thr Leu Val Cys Ser Asn Lys Cys Gly Gly Arg Gly
50 55 60
<210> 113
<211> 186
<212> DNA
<213> 粪肠球菌
<400> 113
atgactgaac ttaacaaaag attacaatta aaaagagatg tttcaacaga aaatagtttg 60
aaaaaaattt ctaatactga tgaaacacat gggggagtta ctacatcaat tccatgtaca 120
gtaatggtta gtgcggcagt atgtcctacc cttgtttgct cgaataaatg tggcggtaga 180
ggctag 186
<210> 114
<211> 58
<212> PRT
<213> 屎肠球菌
<400> 114
Met Gln Asn Val Lys Glu Val Ser Val Lys Glu Met Lys Gln Ile Ile
1 5 10 15
Gly Gly Ser Asn Asp Ser Leu Trp Tyr Gly Val Gly Gln Phe Met Gly
20 25 30
Lys Gln Ala Asn Cys Ile Thr Asn His Pro Val Lys His Met Ile Ile
35 40 45
Pro Gly Tyr Cys Leu Ser Lys Ile Leu Gly
50 55
<210> 115
<211> 177
<212> DNA
<213> 屎肠球菌
<400> 115
atgcaaaatg taaaagaagt ttctgtaaaa gagatgaaac aaattatcgg tggttctaat 60
gatagtcttt ggtatggtgt aggacaattt atgggtaaac aagcaaactg tataacaaac 120
catcctgtta aacacatgat aattcctgga tattgtttat cgaaaatttt agggtaa 177
<210> 116
<211> 55
<212> PRT
<213> 屎肠球菌
<400> 116
Met Lys Lys Tyr Asn Glu Leu Ser Lys Lys Glu Leu Leu Gln Ile Gln
1 5 10 15
Gly Gly Ile Ala Pro Ile Ile Val Ala Gly Leu Gly Tyr Leu Val Lys
20 25 30
Asp Ala Trp Asp His Ser Asp Gln Ile Ile Ser Gly Phe Lys Lys Gly
35 40 45
Trp Asn Gly Gly Arg Arg Lys
50 55
<210> 117
<211> 168
<212> DNA
<213> 屎肠球菌
<400> 117
atgaaaaaat ataatgagtt atctaaaaaa gaacttctac agattcaagg aggaatagca 60
cctattatag ttgctggcct tggctattta gtaaaagatg catgggatca ctcagatcaa 120
ataatctcag gatttaaaaa aggttggaat ggtggacgta gaaaataa 168
<210> 118
<211> 343
<212> PRT
<213> 粪肠球菌
<400> 118
Met Lys Asn Ile Leu Leu Ser Ile Leu Gly Val Leu Ser Ile Val Val
1 5 10 15
Ser Leu Ala Phe Ser Ser Tyr Ser Val Asn Ala Ala Ser Asn Glu Trp
20 25 30
Ser Trp Pro Leu Gly Lys Pro Tyr Ala Gly Arg Tyr Glu Glu Gly Gln
35 40 45
Gln Phe Gly Asn Thr Ala Phe Asn Arg Gly Gly Thr Tyr Phe His Asp
50 55 60
Gly Phe Asp Phe Gly Ser Ala Ile Tyr Gly Asn Gly Ser Val Tyr Ala
65 70 75 80
Val His Asp Gly Lys Ile Leu Tyr Ala Gly Trp Asp Pro Val Gly Gly
85 90 95
Gly Ser Leu Gly Ala Phe Ile Val Leu Gln Ala Gly Asn Thr Asn Val
100 105 110
Ile Tyr Gln Glu Phe Ser Arg Asn Val Gly Asp Ile Lys Val Ser Thr
115 120 125
Gly Gln Thr Val Lys Lys Gly Gln Leu Ile Gly Lys Phe Thr Ser Ser
130 135 140
His Leu His Leu Gly Met Thr Lys Lys Glu Trp Arg Ser Ala His Ser
145 150 155 160
Ser Trp Asn Lys Asp Asp Gly Thr Trp Phe Asn Pro Ile Pro Ile Leu
165 170 175
Gln Gly Gly Ser Thr Pro Thr Pro Pro Asn Pro Gly Pro Lys Asn Phe
180 185 190
Thr Thr Asn Val Arg Tyr Gly Leu Arg Val Leu Gly Gly Ser Trp Leu
195 200 205
Pro Glu Val Thr Asn Phe Asn Asn Thr Asn Asp Gly Phe Ala Gly Tyr
210 215 220
Pro Asn Arg Gln His Asp Met Leu Tyr Ile Lys Val Asp Lys Gly Gln
225 230 235 240
Met Lys Tyr Arg Val His Thr Ala Gln Ser Gly Trp Leu Pro Trp Val
245 250 255
Ser Lys Gly Asp Lys Ser Asp Thr Val Asn Gly Ala Ala Gly Met Pro
260 265 270
Gly Gln Ala Ile Asp Gly Val Gln Leu Asn Tyr Ile Thr Pro Lys Gly
275 280 285
Glu Lys Leu Ser Gln Ala Tyr Tyr Arg Ser Gln Thr Thr Lys Arg Ser
290 295 300
Gly Trp Leu Lys Val Ser Ala Asp Asn Gly Ser Ile Pro Gly Leu Asp
305 310 315 320
Ser Tyr Ala Gly Ile Phe Gly Glu Pro Leu Asp Arg Leu Gln Ile Gly
325 330 335
Ile Ser Gln Ser Asn Pro Phe
340
<210> 119
<211> 1032
<212> DNA
<213> 粪肠球菌
<400> 119
atgaaaaata ttttactttc tattctaggg gtattatcta tcgttgtttc tttggcgttt 60
tcttcttatt ctgtcaacgc agcttctaat gagtggtcgt ggccactggg caaaccatat 120
gcgggaagat atgaagaagg acaacaattc gggaacactg catttaaccg aggaggtact 180
tatttccatg atgggtttga ctttggttct gctatttatg gaaatggcag tgtgtatgct 240
gtgcatgatg gtaaaatttt atatgctggt tgggatcctg taggtggagg ctcattaggt 300
gcatttattg tactacaagc gggaaacaca aatgtgattt atcaagaatt tagccgaaat 360
gttggagata ttaaagttag cactggacaa actgttaaaa aaggacagct gataggaaag 420
tttacttcta gtcatttaca tttaggaatg acaaaaaaag aatggcgttc tgctcattct 480
tcttggaata aagatgatgg cacttggttt aacccaattc ctatacttca aggaggatct 540
acgcctacgc ctccaaatcc aggaccaaaa aatttcacaa caaatgttcg ttacggattg 600
cgggtcctcg gaggttcatg gttaccagaa gtaaccaact ttaacaatac caatgatggt 660
ttcgcaggtt accctaatcg tcaacatgat atgctttata taaaggtaga taaagggcaa 720
atgaaatatc gtgttcacac ggctcaaagt ggatggttgc cttgggtaag taaaggggat 780
aagagcgata cagtaaatgg agcggcaggt atgcctggac aagcaattga tggtgttcag 840
ctaaactata taactcctaa gggagaaaaa ttatcacagg cttactatcg ttcacaaact 900
acgaaacgat caggctggtt aaaagtaagt gcagataatg gttctattcc tggactagac 960
agttatgcag gaatctttgg agaaccgttg gatcgcttgc aaataggtat ttcacagtca 1020
aatccatttt aa 1032
<210> 120
<211> 56
<212> PRT
<213> 表皮葡萄球菌
<400> 120
Met Glu Asn Lys Lys Asp Leu Phe Asp Leu Glu Ile Lys Lys Asp Asn
1 5 10 15
Met Glu Asn Asn Asn Glu Leu Glu Ala Gln Ser Leu Gly Pro Ala Ile
20 25 30
Lys Ala Thr Arg Gln Val Cys Pro Lys Ala Thr Arg Phe Val Thr Val
35 40 45
Ser Cys Lys Lys Ser Asp Cys Gln
50 55
<210> 121
<211> 171
<212> DNA
<213> 表皮葡萄球菌
<400> 121
atggaaaaca aaaaagattt atttgattta gaaatcaaaa aagataatat ggaaaataat 60
aatgaattag aagctcaatc tcttggtcct gcaattaagg caactagaca ggtatgtcct 120
aaagcaacac gttttgttac agtttcttgt aaaaaaagtg attgtcaata g 171
<210> 122
<211> 51
<212> PRT
<213> 表皮葡萄球菌
<400> 122
Met Ala Ala Phe Met Lys Leu Ile Gln Phe Leu Ala Thr Lys Gly Gln
1 5 10 15
Lys Tyr Val Ser Leu Ala Trp Lys His Lys Gly Thr Ile Leu Lys Trp
20 25 30
Ile Asn Ala Gly Gln Ser Phe Glu Trp Ile Tyr Lys Gln Ile Lys Lys
35 40 45
Leu Trp Ala
50
<210> 123
<211> 156
<212> DNA
<213> 表皮葡萄球菌
<400> 123
atggcagcat ttatgaagtt aattcagttc ttagcaacta aaggtcaaaa gtatgtttca 60
cttgcatgga aacataaagg tactatttta aaatggatta acgccggtca aagttttgaa 120
tggatttata aacaaatcaa aaaattatgg gcataa 156
<210> 124
<211> 52
<212> PRT
<213> 表皮葡萄球菌
<400> 124
Met Glu Ala Val Lys Glu Lys Asn Asp Leu Phe Asn Leu Asp Val Lys
1 5 10 15
Val Asn Ala Lys Glu Ser Asn Asp Ser Gly Ala Glu Pro Arg Ile Ala
20 25 30
Ser Lys Phe Ile Cys Thr Pro Gly Cys Ala Lys Thr Gly Ser Phe Asn
35 40 45
Ser Tyr Cys Cys
50
<210> 125
<211> 159
<212> DNA
<213> 表皮葡萄球菌
<400> 125
atggaagcag taaaagaaaa aaatgatctt tttaatcttg atgttaaagt taatgcaaaa 60
gaatctaacg attcaggagc tgaaccaaga attgctagta aatttatatg tactcctgga 120
tgtgcaaaaa caggtagttt taacagttat tgttgttaa 159
<210> 126
<211> 55
<212> PRT
<213> 表皮葡萄球菌
<400> 126
Met Asn Asn Ser Leu Phe Asp Leu Asn Leu Asn Lys Gly Val Glu Thr
1 5 10 15
Gln Lys Ser Asp Leu Ser Pro Gln Ser Ala Ser Val Leu Lys Thr Ser
20 25 30
Ile Lys Val Ser Lys Lys Tyr Cys Lys Gly Val Thr Leu Thr Cys Gly
35 40 45
Cys Asn Ile Thr Gly Gly Lys
50 55
<210> 127
<211> 168
<212> DNA
<213> 表皮葡萄球菌
<400> 127
atgaataact cattattcga tttaaaccta aacaaaggtg tagaaactca aaagagtgat 60
ttaagtccgc aatctgctag tgtcttgaag acttctatta aagtatctaa aaaatattgt 120
aaaggtgtta ctttaacatg cggttgcaat attactggtg gtaaataa 168
<210> 128
<211> 52
<212> PRT
<213> 鸡葡萄球菌
<400> 128
Met Glu Ala Val Lys Glu Lys Asn Glu Leu Phe Asp Leu Asp Val Lys
1 5 10 15
Val Asn Ala Lys Glu Ser Asn Asp Ser Gly Ala Glu Pro Arg Ile Ala
20 25 30
Ser Lys Phe Leu Cys Thr Pro Gly Cys Ala Lys Thr Gly Ser Phe Asn
35 40 45
Ser Tyr Cys Cys
50
<210> 129
<211> 159
<212> DNA
<213> 鸡葡萄球菌
<400> 129
atggaagcag taaaagagaa aaatgaactt tttgatcttg acgttaaagt aaatgcaaaa 60
gagtctaatg attcaggcgc agaaccacga attgctagta aatttttatg tactcctgga 120
tgtgccaaaa caggtagctt caatagctac tgttgttaa 159
<210> 130
<211> 63
<212> PRT
<213> 格氏乳球菌
<400> 130
Met Glu Asn Asn Asn Tyr Thr Val Leu Ser Asp Glu Glu Leu Gln Lys
1 5 10 15
Ile Asp Gly Gly Ile Gly Gly Ala Leu Gly Asn Ala Leu Asn Gly Leu
20 25 30
Gly Thr Trp Ala Asn Met Met Asn Gly Gly Gly Phe Val Asn Gln Trp
35 40 45
Gln Val Tyr Ala Asn Lys Gly Lys Ile Asn Gln Tyr Arg Pro Tyr
50 55 60
<210> 131
<211> 192
<212> DNA
<213> 格氏乳球菌
<400> 131
atggaaaaca acaattacac agtactttca gatgaagaac tacaaaaaat tgatggtgga 60
atcggcgggg ctcttggtaa tgctctcaac ggattaggta cctgggcaaa catgatgaac 120
ggtggaggat ttgttaatca gtggcaagtt tatgctaata aaggaaaaat aaatcaatac 180
cgtccgtatt aa 192
<210> 132
<211> 63
<212> PRT
<213> 格氏乳球菌
<400> 132
Met Phe Asp Leu Val Ala Thr Gly Met Ala Ala Gly Val Ala Lys Thr
1 5 10 15
Ile Val Asn Ala Val Ser Ala Gly Met Asp Ile Ala Thr Ala Leu Ser
20 25 30
Leu Phe Ser Gly Ala Phe Thr Ala Ala Gly Gly Ile Met Ala Leu Ile
35 40 45
Lys Lys Tyr Ala Gln Lys Lys Leu Trp Lys Gln Leu Ile Ala Ala
50 55 60
<210> 133
<211> 192
<212> DNA
<213> 格氏乳球菌
<400> 133
atgtttgatt tagtcgcgac tggaatggct gcaggtgtag caaaaactat tgttaatgcc 60
gttagtgctg gtatggatat tgccactgct ttatcattgt tctcaggagc ttttactgca 120
gctgggggaa ttatggcact cattaaaaaa tatgctcaaa agaaattatg gaaacagctt 180
attgctgcat aa 192
<210> 134
<211> 91
<212> PRT
<213> 加氏乳杆菌
<400> 134
Met Val Thr Lys Tyr Gly Arg Asn Leu Gly Leu Asn Lys Val Glu Leu
1 5 10 15
Phe Ala Ile Trp Ala Val Leu Val Val Ala Leu Leu Leu Thr Thr Ala
20 25 30
Asn Ile Tyr Trp Ile Ala Asp Gln Phe Gly Ile His Leu Ala Thr Gly
35 40 45
Thr Ala Arg Lys Leu Leu Asp Ala Met Ala Ser Gly Ala Ser Leu Gly
50 55 60
Thr Ala Phe Ala Ala Ile Leu Gly Val Thr Leu Pro Ala Trp Ala Leu
65 70 75 80
Ala Ala Ala Gly Ala Leu Gly Ala Thr Ala Ala
85 90
<210> 135
<211> 276
<212> DNA
<213> 加氏乳杆菌
<400> 135
atggttacta agtacggacg taatttaggt ttgaacaagg tagagttgtt tgcaatttgg 60
gcggttttag tagttgctct tttattgacc acagcgaaca tttattggat tgctgatcaa 120
ttcgggattc atttagcgac tggaacagcc cgtaagttat tagatgcaat ggcttctggt 180
gcctcattgg gaactgcctt tgctgctatt ttgggcgtga cattacctgc atgggctttg 240
gcagctgcag gagcattggg agcgactgca gcctag 276
<210> 136
<211> 75
<212> PRT
<213> 加氏乳杆菌
<400> 136
Met Lys Asn Phe Asn Thr Leu Ser Phe Glu Thr Leu Ala Asn Ile Val
1 5 10 15
Gly Gly Arg Asn Asn Trp Ala Ala Asn Ile Gly Gly Val Gly Gly Ala
20 25 30
Thr Val Ala Gly Trp Ala Leu Gly Asn Ala Val Cys Gly Pro Ala Cys
35 40 45
Gly Phe Val Gly Ala His Tyr Val Pro Ile Ala Trp Ala Gly Val Thr
50 55 60
Ala Ala Thr Gly Gly Phe Gly Lys Ile Arg Lys
65 70 75
<210> 137
<211> 228
<212> DNA
<213> 加氏乳杆菌
<400> 137
atgaaaaatt ttaatacatt atcatttgaa acattggcta acatagttgg tgggagaaat 60
aattgggctg ctaatatagg tggagtaggt ggagcgacag tcgctggatg ggctcttgga 120
aatgcagttt gcggtcctgc ttgtggcttt gttggagcac actatgttcc aatagcatgg 180
gctggcgtaa cggcagctac tggtggattc ggaaagataa gaaagtag 228
<210> 138
<211> 64
<212> PRT
<213> 植物乳杆菌
<400> 138
Met Ser Lys Leu Val Lys Thr Leu Thr Ile Ser Glu Ile Ser Lys Ala
1 5 10 15
Gln Asn Asn Gly Gly Lys Pro Ala Trp Cys Trp Tyr Thr Leu Ala Met
20 25 30
Cys Gly Ala Gly Tyr Asp Ser Gly Thr Cys Asp Tyr Met Tyr Ser His
35 40 45
Cys Phe Gly Ile Lys His His Ser Ser Gly Ser Ser Ser Tyr His Cys
50 55 60
<210> 139
<211> 195
<212> DNA
<213> 植物乳杆菌
<400> 139
atgagtaaat tggttaagac acttactata agtgaaattt ctaaggctca aaacaacggt 60
ggaaaacctg catggtgttg gtatacttta gcaatgtgtg gtgctggtta tgattcggga 120
acctgtgatt atatgtattc gcattgtttt ggtataaagc atcatagtag tggtagtagc 180
agttatcatt gttag 195
<210> 140
<211> 359
<212> PRT
<213> 地中海嗜盐菌
<400> 140
Met Ser Lys Asp Arg Asp Gly Arg Arg Thr Ser Arg Arg Gly Thr Leu
1 5 10 15
Lys Lys Ile Gly Gly Phe Ser Leu Gly Ala Leu Ser Phe Gly Ala Val
20 25 30
Gly Arg Thr Gln Ala Ala Thr Gly Ser Ser Val Thr Thr Ala Asp Ile
35 40 45
Ala Pro Pro Gly Pro Asn Gly Asp Pro Lys Ser Val Gln Ile Asp Asp
50 55 60
Lys Tyr Thr Gly Ala Glu Met Tyr Gly Glu Gly Asp Phe Arg Val Gly
65 70 75 80
Leu Gly Thr Asp Leu Thr Met Tyr Pro Pro Val Tyr Arg Glu Ser Leu
85 90 95
Gly Asn Gly Ser Gly Gly Trp Glu Phe Asp Phe Thr Val Cys Gly Ser
100 105 110
Thr Ala Cys Arg Phe Val Asp Ser Asn Gly Asp Val Lys Glu Asp Asp
115 120 125
Lys Ala Lys Glu Met Trp Trp Gln Glu Ile Asn Phe Asn Asp Ile Asn
130 135 140
Gln Asp Leu Tyr Ser Arg Asn Asp Ser Asp Trp Val Gly Ser Thr Pro
145 150 155 160
Ala Asp Thr Gln Pro Glu Phe Asp Tyr Thr Glu Phe Ala Leu Ala Arg
165 170 175
Asp Gly Val Thr Leu Ala Leu Thr Ala Leu Asn Pro Ala Met Gly Ser
180 185 190
Leu Ala Leu Gly Ala Thr Tyr Phe Leu Ser Asp Met Val Asn Trp Ile
195 200 205
Ala Ser Gln His Glu Asp Asp Ser Ser Leu Lys Arg Lys Trp Asp Tyr
210 215 220
Asp Gly Leu Ser Gly Pro Leu Tyr Ala Asp Ser Ser Thr Tyr Leu Leu
225 230 235 240
Ala Arg Asp Glu Met Thr Ser Asn Ser Tyr Glu Ser Phe Thr Ile Asp
245 250 255
Asn Ile Ala Val Ala Phe Pro Glu Phe Pro Val Arg Thr Lys Tyr Tyr
260 265 270
Val Thr Phe Thr Ala Pro Asp Asp Pro Ser Thr Gln Ser Ile Ser Thr
275 280 285
Leu Glu Glu Glu Gly Ile Tyr Arg Val Pro Ala Thr Glu Val Ala Ala
290 295 300
Ala Arg Pro Pro Gly Ser Arg Arg Ser Lys Ser Ala Ala Asp Glu Met
305 310 315 320
Val Tyr Val Ala Asp Pro Lys Lys Phe Ile Glu Val Glu Pro Val Lys
325 330 335
Asn Pro Ser Ile Pro Asp Arg Ile Tyr Glu Glu Ile Glu Gln Lys Lys
340 345 350
Lys Gln Arg Ser Arg Lys Gln
355
<210> 141
<211> 1080
<212> DNA
<213> 地中海嗜盐菌
<400> 141
atgtcgaaag acagagatgg gagaaggaca agtcggcgag gcacgttaaa gaaaatcggc 60
ggtttcagtc tcggagcgct tagtttcggg gcagtcggac gaactcaagc ggcgaccggc 120
tcatcggtta cgaccgctga tatcgcacct cccggaccga acggagaccc gaagagtgtt 180
cagatagatg ataaatacac cggagccgag atgtacggcg agggtgactt cagagtcggt 240
ctcggaactg acctgacgat gtatccgccc gtgtaccgtg agagtcttgg aaatggaagc 300
gggggttggg aattcgactt caccgtttgt gggtccactg cctgtcgatt tgtggacagt 360
aacggtgacg tcaaagagga cgacaaggcg aaagaaatgt ggtggcagga aattaacttc 420
aacgacataa atcaggattt atacagtcgg aacgattccg actgggtcgg gtcgacccct 480
gccgataccc aaccggagtt cgattacacc gactttgcgc tcgctcggga cggagtgacg 540
ctcgctctca cggcactcaa ccccgcaatg gggagtcttg cactcggtgc cacgtacttc 600
ctcagcgaca tggtgaactg gattgcgagc cagcacgaag acgacagttc gctcaagaga 660
aaatgggatt acgacgggct aagtgggccg ttgtacgccg attcgtcgac gtacctactg 720
gcacgcgacg agatgacttc gaactcgtac gaatcattca cgatcgataa catcgccgtt 780
gccttcccag agttccccgt ccggaccaag tactacgtca cattcactgc gccggatgac 840
ccgtcaacgc agtcgatatc tacgctcgaa gaggagggaa tctaccgagt gcccgctacg 900
gaagtggctg cggccagacc accggggtcc cgacgttcca aatcggcagc cgacgagatg 960
gtgtacgttg ccgatccgaa gaagttcata gaggtcgagc cggtgaagaa cccaagtatc 1020
ccggaccgaa tctacgagga gatagagcaa aaaaagaaac aacggagtag gaaacagtag 1080
<210> 142
<211> 311
<212> PRT
<213> Haloarchaeon S8a
<400> 142
Met Ser Asp Lys Asp Ser Ile Asn Arg Arg Asn Val Leu Arg Lys Ile
1 5 10 15
Gly Gly Ile Gly Val Ala Ser Ala Val Gly Phe Ser Gly Leu Ala Ser
20 25 30
Gly Glu Ser Leu Ser Asp Asp Glu Lys Gln Asp Val Ile Asp Thr Ile
35 40 45
Tyr Lys Ser Gln Arg Val Glu Gln Ile Lys Lys Lys Phe Gly Gly Val
50 55 60
Asn Ile Glu Pro Lys Lys Val Gln Ser Val Thr Thr Asn Gln Ser Gly
65 70 75 80
Asp Leu Val Thr Ala Lys Leu Ser Val Ser Asp Gly Asp Leu Val Tyr
85 90 95
Ser Ser Val Lys Asp Thr Thr Val Ile Val Gln Phe Asp Arg Ser Ala
100 105 110
Ser Glu Ile Gly Glu Ser Trp Pro Lys Asn Thr Glu Ala Phe Ile Lys
115 120 125
Ser Thr Ser Ser Gly Val Asp Leu Leu Arg Thr Ala Thr Asp Glu Glu
130 135 140
Ile Lys Asp Val Thr Glu Gly Val Asn Thr Ser Glu Ile Glu Ser Ala
145 150 155 160
Asp Ala Val Asn Ile Phe Ile Asp Pro Glu Ser Gln Thr Tyr Tyr Met
165 170 175
Glu Lys Tyr Asp Phe Asn Asn Lys Val Leu Glu Met Phe Glu Leu Ala
180 185 190
Thr Gly Gly Thr Ser Ser Gly Lys Ile Ser Pro Thr Arg Glu Asp Gln
195 200 205
Asn His Glu Tyr Asn Val Arg Glu His Lys Val Phe Asn Ser Glu Lys
210 215 220
Gln Asn Ile Gln Leu Gln Ser Asp Cys Asn Ile Asn Ser Asn Thr Ala
225 230 235 240
Ala Asp Val Ile Leu Cys Phe Asn Gln Val Gly Ser Cys Ala Leu Cys
245 250 255
Ser Pro Thr Leu Val Gly Gly Pro Val Pro Thr Val Ala Cys Leu Leu
260 265 270
Val Val Cys Phe Gly Thr Pro Asn Ala Val Ser Ala Ile Leu Glu Glu
275 280 285
Val Asp Asn Ser Cys Phe Asn Leu Ile Lys Asp Val Ile Ser Cys Trp
290 295 300
Asp Glu Trp Thr Ser Phe Trp
305 310
<210> 143
<211> 936
<212> DNA
<213> Haloarchaeon S8a
<400> 143
atgtcggata aagacagcat taacagaaga aatgtattaa gaaaaattgg cggtatcggt 60
gtggcttcag ctgtcggatt ttctggtttg gcaagcgggg aaagtcttag cgatgatgag 120
aaacaagatg ttattgacac aatttacaaa tcacaaagag ttgaacagat aaagaaaaag 180
ttcggaggag tgaatattga gccgaaaaag gttcaatctg taacgaccaa tcagagcgga 240
gatcttgtta cggcgaagct gtcggttagt gatggggatt tggtatattc gagtgtcaaa 300
gatacaactg taatagttca gttcgataga tcggcttctg aaattggtga aagttggccc 360
aagaatactg aggcattcat caaatcgacg tcctctgggg tcgatcttct acgtacagca 420
actgatgaag aaataaagga cgttactgag ggagtcaaca catctgaaat tgaatctgcg 480
gatgctgtta acatatttat tgatcctgaa tcacagacat actatatgga gaaatatgac 540
tttaataata aggtacttga gatgtttgaa ttagcgacag gtgggacaag tagtggtaaa 600
atctccccca cacgtgaaga ccagaatcac gaatataatg ttagggaaca taaagtattt 660
aactcagaaa aacagaatat acaacttcag agtgactgta atataaacag taacaccgct 720
gctgatgtta ttctatgctt caaccaggtt ggttcttgtg cactctgctc cccgacttta 780
gtcggaggtc cagtccctac agttgcatgt ctcttagtcg tctgtttcgg cactccaaat 840
gctgtgtccg cgatacttga agaagtcgat aattcttgct ttaacttgat caaggatgta 900
atttcgtgtt gggatgaatg gactagcttc tggtga 936
<210> 144
<211> 333
<212> PRT
<213> 瑞士乳杆菌
<400> 144
Met Lys His Leu Asn Glu Thr Thr Asn Val Arg Ile Leu Ser Gln Phe
1 5 10 15
Asp Met Asp Thr Gly Tyr Gln Ala Val Val Gln Lys Gly Asn Val Gly
20 25 30
Ser Lys Tyr Val Tyr Gly Leu Gln Leu Arg Lys Gly Ala Thr Thr Ile
35 40 45
Leu Arg Gly Tyr Arg Gly Ser Lys Ile Asn Asn Pro Ile Leu Glu Leu
50 55 60
Ser Gly Gln Ala Gly Gly His Thr Gln Thr Trp Glu Phe Ala Gly Asp
65 70 75 80
Arg Lys Asp Ile Asn Gly Glu Glu Arg Ala Gly Gln Trp Phe Ile Gly
85 90 95
Val Lys Pro Ser Lys Ile Glu Gly Ser Lys Ile Ile Trp Ala Lys Gln
100 105 110
Ile Ala Arg Val Asp Leu Arg Asn Gln Met Gly Pro His Tyr Ser Asn
115 120 125
Thr Asp Phe Pro Arg Leu Ser Tyr Leu Asn Arg Ala Gly Ser Asn Pro
130 135 140
Phe Ala Gly Asn Lys Met Thr His Ala Glu Ala Ala Val Ser Pro Asp
145 150 155 160
Tyr Thr Lys Phe Leu Ile Ala Thr Val Glu Asn Asn Cys Ile Gly His
165 170 175
Phe Thr Ile Tyr Asn Leu Asp Thr Ile Asn Glu Lys Leu Asp Glu Lys
180 185 190
Gly Asn Ser Glu Asp Val Asn Leu Glu Thr Val Lys Tyr Glu Asp Ser
195 200 205
Phe Ile Ile Asp Asn Leu Tyr Gly Asp Asp Asn Asn Ser Ile Val Asn
210 215 220
Ser Ile Gln Gly Tyr Asp Leu Asp Asn Asp Gly Asn Ile Tyr Ile Ser
225 230 235 240
Ser Gln Lys Ala Pro Asp Phe Asp Gly Ser Tyr Tyr Ala His His Lys
245 250 255
Gln Ile Val Lys Ile Pro Tyr Tyr Ala Arg Ser Lys Glu Ser Glu Asp
260 265 270
Gln Trp Arg Ala Val Asn Leu Ser Glu Phe Gly Gly Leu Asp Ile Pro
275 280 285
Gly Lys His Ser Glu Val Glu Ser Ile Gln Ile Ile Gly Glu Asn His
290 295 300
Cys Tyr Leu Thr Val Ala Tyr His Ser Lys Asn Lys Ala Gly Glu Asn
305 310 315 320
Lys Thr Thr Leu Asn Glu Ile Tyr Glu Leu Ser Trp Asn
325 330
<210> 145
<211> 1002
<212> DNA
<213> 瑞士乳杆菌
<400> 145
atgaagcatt taaatgaaac aactaatgtt agaattttaa gtcaatttga tatggatact 60
ggctatcaag cagtagttca aaaaggcaat gtaggttcaa aatatgtata tggattacaa 120
cttcgcaaag gtgctactac tatcttgcgt ggttaccgtg gaagtaaaat taataaccct 180
attcttgaat tatctggtca agcaggtggt cacacacaga catgggaatt tgctggtgat 240
cgtaaagaca ttaatggtga agaaagagca ggtcaatggt ttataggtgt taaaccatcg 300
aaaattgaag gaagcaaaat tatttgggca aagcaaattg caagagttga tcttagaaat 360
caaatgggac ctcattattc aaatactgac tttcctcgat tatcctactt gaatcgcgcc 420
ggttctaatc catttgctgg taataagatg acgcatgccg aagccgcagt atcacctgat 480
tatactaagt ttttaattgc tactgttgaa aataactgta ttggtcattt tactatatac 540
aatttagata caattaatga aaaacttgat gaaaagggaa atagtgaaga tgttaatctc 600
gaaactgtta aatacgaaga tagttttatc attgataatt tatatggtga tgataataat 660
tctattgtaa attcaattca agggtatgat ttggataatg atggaaatat ttatatttcc 720
agtcaaaaag cgccagattt tgatggctct tattatgcac atcataagca gattgttaag 780
attccatatt atgctcggtc taaagaaagc gaagaccaat ggagagctgt aaatttaagc 840
gaattcggtg gcttggatat tccaggtaaa catagtgaag ttgaaagcat ccaaattatt 900
ggtgagaatc attgttactt aactgttgca tatcattcta aaaataaagc gggtgaaaat 960
aaaactactt tgaatgagat ttatgaatta tcttggaatt ag 1002
<210> 146
<211> 74
<212> PRT
<213> 海氏肠球菌
<400> 146
Met Lys Lys Lys Val Leu Lys His Cys Val Ile Leu Gly Ile Leu Gly
1 5 10 15
Thr Cys Leu Ala Gly Ile Gly Thr Gly Ile Lys Val Asp Ala Ala Thr
20 25 30
Tyr Tyr Gly Asn Gly Leu Tyr Cys Asn Lys Glu Lys Cys Trp Val Asp
35 40 45
Trp Asn Gln Ala Lys Gly Glu Ile Gly Lys Ile Ile Val Asn Gly Trp
50 55 60
Val Asn His Gly Pro Trp Ala Pro Arg Arg
65 70
<210> 147
<211> 225
<212> DNA
<213> 海氏肠球菌
<400> 147
atgaaaaaga aagtattaaa acattgtgtt attctaggaa tattaggaac ttgtctagct 60
ggcatcggta caggaataaa agttgatgca gctacttact atggaaatgg tctttattgt 120
aacaaagaaa aatgttgggt agattggaat caagctaaag gagaaattgg aaaaattatt 180
gttaatggtt gggttaatca tggtccatgg gcacctagaa ggtag 225
<210> 148
<211> 75
<212> PRT
<213> 约氏乳酸杆菌
<400> 148
Met Lys Gln Phe Asn Tyr Leu Ser His Lys Asp Leu Ala Val Val Val
1 5 10 15
Gly Gly Arg Asn Asn Trp Gln Thr Asn Val Gly Gly Ala Val Gly Ser
20 25 30
Ala Met Ile Gly Ala Thr Val Gly Gly Thr Ile Cys Gly Pro Ala Cys
35 40 45
Ala Val Ala Gly Ala His Tyr Leu Pro Ile Leu Trp Thr Ala Val Thr
50 55 60
Ala Ala Thr Gly Gly Phe Gly Lys Ile Arg Lys
65 70 75
<210> 149
<211> 228
<212> DNA
<213> 约氏乳酸杆菌
<400> 149
atgaaacaat ttaattattt atcacataaa gatttagcag tcgttgttgg tggaagaaat 60
aattggcaaa caaatgtggg aggagcagtg ggatcagcta tgattggggc tacagttggt 120
ggtacaattt gtggacctgc atgtgctgta gctggtgccc attatcttcc tattttatgg 180
acagcggtta cagctgcaac aggtggtttt ggcaagataa gaaagtag 228
<210> 150
<211> 62
<212> PRT
<213> 约氏乳酸杆菌
<400> 150
Met Lys Leu Asn Asp Lys Glu Leu Ser Lys Ile Val Gly Gly Asn Arg
1 5 10 15
Trp Gly Asp Thr Val Leu Ser Ala Ala Ser Gly Ala Gly Thr Gly Ile
20 25 30
Lys Ala Cys Lys Ser Phe Gly Pro Trp Gly Met Ala Ile Cys Gly Val
35 40 45
Gly Gly Ala Ala Ile Gly Gly Tyr Phe Gly Tyr Thr His Asn
50 55 60
<210> 151
<211> 189
<212> DNA
<213> 约氏乳酸杆菌
<400> 151
atgaaattaa atgacaaaga attatcaaag attgttggtg gaaatcgatg gggagatact 60
gttttatcag ctgctagtgg cgcaggaact ggtattaaag catgtaaaag ttttggccca 120
tggggaatgg caatttgtgg tgtaggaggt gcagcaatag gaggttattt tggctatact 180
cataattaa 189
<210> 152
<211> 59
<212> PRT
<213> 乳酸乳球菌乳酸亚种
<400> 152
Met Asn Lys Asn Glu Ile Glu Thr Gln Pro Val Thr Trp Leu Glu Glu
1 5 10 15
Val Ser Asp Gln Asn Phe Asp Glu Asp Val Phe Gly Ala Cys Ser Thr
20 25 30
Asn Thr Phe Ser Leu Ser Asp Tyr Trp Gly Asn Asn Gly Ala Trp Cys
35 40 45
Thr Leu Thr His Glu Cys Met Ala Trp Cys Lys
50 55
<210> 153
<211> 180
<212> DNA
<213> 乳酸乳球菌乳酸亚种
<400> 153
atgaacaaaa atgaaattga aacacaacca gttacatggt tggaagaagt atctgatcaa 60
aattttgatg aagatgtatt tggtgcgtgt agtactaaca cattctcgct cagtgattac 120
tggggaaata acggggcttg gtgtacactc actcatgaat gtatggcttg gtgtaaataa 180
<210> 154
<211> 65
<212> PRT
<213> 乳酸乳球菌乳酸亚种
<400> 154
Met Lys Glu Lys Asn Met Lys Lys Asn Asp Thr Ile Glu Leu Gln Leu
1 5 10 15
Gly Lys Tyr Leu Glu Asp Asp Met Ile Glu Leu Ala Glu Gly Asp Glu
20 25 30
Ser His Gly Gly Thr Thr Pro Ala Thr Pro Ala Ile Ser Ile Leu Ser
35 40 45
Ala Tyr Ile Ser Thr Asn Thr Cys Pro Thr Thr Lys Cys Thr Arg Ala
50 55 60
Cys
65
<210> 155
<211> 198
<212> DNA
<213> 乳酸乳球菌乳酸亚种
<400> 155
atgaaagaaa aaaatatgaa aaagaatgac actattgaat tacaattggg aaaatacctt 60
gaagatgata tgattgaatt agctgaaggg gatgagtctc atggaggaac aacaccagca 120
actcctgcaa tctctattct cagtgcatat attagtacca atacttgtcc aacaacaaaa 180
tgtacacgtg cttgttaa 198
<210> 156
<211> 51
<212> PRT
<213> 乳酸乳球菌乳酸亚种
<400> 156
Met Lys Glu Gln Asn Ser Phe Asn Leu Leu Gln Glu Val Thr Glu Ser
1 5 10 15
Glu Leu Asp Leu Ile Leu Gly Ala Lys Gly Gly Ser Gly Val Ile His
20 25 30
Thr Ile Ser His Glu Cys Asn Met Asn Ser Trp Gln Phe Val Phe Thr
35 40 45
Cys Cys Ser
50
<210> 157
<211> 156
<212> DNA
<213> 乳酸乳球菌乳酸亚种
<400> 157
atgaaagaac aaaactcttt taatcttctt caagaagtga cagaaagtga attggacctt 60
attttaggtg caaaaggcgg cagtggagtt attcatacaa tttctcatga atgtaatatg 120
aatagctggc aatttgtatt tacttgctgc tcttaa 156
<210> 158
<211> 53
<212> PRT
<213> 乳酸乳球菌
<400> 158
Met Ala Gly Phe Leu Lys Val Val Gln Leu Leu Ala Lys Tyr Gly Ser
1 5 10 15
Lys Ala Val Gln Trp Ala Trp Ala Asn Lys Gly Lys Ile Leu Asp Trp
20 25 30
Leu Asn Ala Gly Gln Ala Ile Asp Trp Val Val Ser Lys Ile Lys Gln
35 40 45
Ile Leu Gly Ile Lys
50
<210> 159
<211> 162
<212> DNA
<213> 乳酸乳球菌
<400> 159
atggcagggt ttttaaaagt agttcaatta ctagctaaat atggttctaa agctgtacaa 60
tgggcttggg caaacaaggg taagatttta gattggctta atgcaggtca ggctattgat 120
tgggtagttt cgaaaattaa gcaaatttta ggtattaagt aa 162
<210> 160
<211> 53
<212> PRT
<213> 乳酸乳球菌
<400> 160
Met Ala Gly Phe Leu Lys Val Val Gln Ile Leu Ala Lys Tyr Gly Ser
1 5 10 15
Lys Ala Val Gln Trp Ala Trp Ala Asn Lys Gly Lys Ile Leu Asp Trp
20 25 30
Ile Asn Ala Gly Gln Ala Ile Asp Trp Val Val Glu Lys Ile Lys Gln
35 40 45
Ile Leu Gly Ile Lys
50
<210> 161
<211> 162
<212> DNA
<213> 乳酸乳球菌
<400> 161
atggcagggt ttttaaaagt agtccaaatt ttggctaagt atggttctaa agccgtacaa 60
tgggcatggg caaataaagg aaaaatctta gattggatta atgcaggtca agctattgac 120
tgggtagttg aaaagattaa gcaaattttg ggtattaaat aa 162
<210> 162
<211> 65
<212> PRT
<213> 淀粉乳杆菌
<400> 162
Met Lys Gln Leu Asn Ser Glu Gln Leu Gln Asn Ile Ile Gly Gly Asn
1 5 10 15
Arg Trp Thr Asn Ala Tyr Ser Ala Ala Leu Gly Cys Ala Val Pro Gly
20 25 30
Val Lys Tyr Gly Lys Lys Leu Gly Gly Val Trp Gly Ala Val Ile Gly
35 40 45
Gly Val Gly Gly Ala Ala Val Cys Gly Leu Ala Gly Tyr Val Arg Lys
50 55 60
Gly
65
<210> 163
<211> 198
<212> DNA
<213> 淀粉乳杆菌
<400> 163
atgaaacaat tgaattcaga acaattacaa aatattatcg gtggaaatag atggactaat 60
gcatacagcg cagctttggg atgcgctgtc cctggagtta aatatggaaa aaaacttggt 120
ggcgtatggg gtgctgtaat tggtggcgta ggcggtgcag cagtctgtgg cttggcgggt 180
tatgttcgta aaggctaa 198
<210> 164
<211> 68
<212> PRT
<213> 清酒乳杆菌L45
<400> 164
Met Lys Thr Glu Lys Lys Val Leu Asp Glu Leu Ser Leu His Ala Ser
1 5 10 15
Ala Lys Met Gly Ala Arg Asp Val Glu Ser Ser Met Asn Ala Asp Ser
20 25 30
Thr Pro Val Leu Ala Ser Val Ala Val Ser Met Glu Leu Leu Pro Thr
35 40 45
Ala Ser Val Leu Tyr Ser Asp Val Ala Gly Cys Phe Lys Tyr Ser Ala
50 55 60
Lys His His Cys
65
<210> 165
<211> 207
<212> DNA
<213> 清酒乳杆菌L45
<400> 165
atgaaaacag aaaaaaaggt tttagatgaa ctgagcttac acgcttctgc aaaaatggga 60
gcacgtgatg ttgaatccag catgaatgca gactcaacac cagttttagc atcagtcgct 120
gtatccatgg aattattgcc aactgcgtct gttctttatt cggatgttgc aggttgcttc 180
aaatattctg caaaacatca ttgttag 207
<210> 166
<211> 91
<212> PRT
<213> 乳酸乳球菌乳酸亚种
<400> 166
Met Lys Thr Lys Ser Leu Val Leu Ala Leu Ser Ala Val Thr Leu Phe
1 5 10 15
Ser Ala Gly Gly Ile Val Ala Gln Ala Glu Gly Thr Trp Gln His Gly
20 25 30
Tyr Gly Val Ser Ser Ala Tyr Ser Asn Tyr His His Gly Ser Lys Thr
35 40 45
His Ser Ala Thr Val Val Asn Asn Asn Thr Gly Arg Gln Gly Lys Asp
50 55 60
Thr Gln Arg Ala Gly Val Trp Ala Lys Ala Thr Val Gly Arg Asn Leu
65 70 75 80
Thr Glu Lys Ala Ser Phe Tyr Tyr Asn Phe Trp
85 90
<210> 167
<211> 276
<212> DNA
<213> 乳酸乳球菌乳酸亚种
<400> 167
atgaaaacca agtctctcgt attggcatta tctgcggtta cgttattctc tgccggagga 60
attgtagctc aagctgaagg aacatggcaa catggatatg gtgttagttc ggcatattca 120
aattatcatc atggtagcaa aactcattca gccacagttg taaataataa tactggccga 180
caaggtaagg atacacaacg tgccggtgtt tgggcaaaag ctactgttgg acgtaactta 240
actgaaaaag cttcatttta ttataacttt tggtaa 276
<210> 168
<211> 75
<212> PRT
<213> 乳酸乳球菌乳脂亚种
<400> 168
Met Lys Asn Gln Leu Asn Phe Asn Ile Val Ser Asp Glu Glu Leu Ser
1 5 10 15
Glu Ala Asn Gly Gly Lys Leu Thr Phe Ile Gln Ser Thr Ala Ala Gly
20 25 30
Asp Leu Tyr Tyr Asn Thr Asn Thr His Lys Tyr Val Tyr Gln Gln Thr
35 40 45
Gln Asn Ala Phe Gly Ala Ala Ala Asn Thr Ile Val Asn Gly Trp Met
50 55 60
Gly Gly Ala Ala Gly Gly Phe Gly Leu His His
65 70 75
<210> 169
<211> 228
<212> DNA
<213> 乳酸乳球菌乳脂亚种
<400> 169
atgaaaaatc aattaaattt taatattgtt tcagatgaag aactttcaga agctaacgga 60
ggaaaattaa catttattca atcgacagcg gctggagatt tatattacaa tactaataca 120
cacaaatatg tttaccaaca aactcaaaac gcttttgggg ctgctgctaa taccattgtt 180
aatggatgga tgggtggcgc tgctggaggt ttcgggttgc accattga 228
<210> 170
<211> 68
<212> PRT
<213> 乳酸乳球菌乳脂亚种
<400> 170
Met Lys Asn Gln Leu Asn Phe Asn Ile Val Ser Asp Glu Glu Leu Ala
1 5 10 15
Glu Val Asn Gly Gly Ser Leu Gln Tyr Val Met Ser Ala Gly Pro Tyr
20 25 30
Thr Trp Tyr Lys Asp Thr Arg Thr Gly Lys Thr Ile Cys Lys Gln Thr
35 40 45
Ile Asp Thr Ala Ser Tyr Thr Phe Gly Val Met Ala Glu Gly Trp Gly
50 55 60
Lys Thr Phe His
65
<210> 171
<211> 207
<212> DNA
<213> 乳酸乳球菌乳脂亚种
<400> 171
atgaaaaatc aattaaattt taatattgtt tctgatgaag aacttgcaga agttaatgga 60
ggaagcttgc agtatgttat gagtgctgga ccatatactt ggtataaaga tactagaaca 120
ggaaaaacaa tatgtaaaca gacaattgac acagcaagtt atacatttgg tgtaatggca 180
gaaggatggg gaaaaacatt ccactaa 207
<210> 172
<211> 63
<212> PRT
<213> 乳球菌QU 12
<400> 172
Met Lys Leu Ile Asp His Leu Gly Ala Pro Arg Trp Ala Val Asp Thr
1 5 10 15
Ile Leu Gly Ala Ile Ala Val Gly Asn Leu Ala Ser Trp Val Leu Ala
20 25 30
Leu Val Pro Gly Pro Gly Trp Ala Val Lys Ala Gly Leu Ala Thr Ala
35 40 45
Ala Ala Ile Val Lys His Gln Gly Lys Ala Ala Ala Ala Ala Trp
50 55 60
<210> 173
<211> 192
<212> DNA
<213> 乳球菌QU 12
<400> 173
atgaaattaa ttgatcattt aggtgctcca agatgggccg ttgatactat tttaggtgca 60
atcgcagttg ggaacttagc aagttgggtt ctagcgcttg tccctggtcc agggtgggca 120
gtaaaagctg gtttagcaac tgctgctgcc atcgttaaac atcaaggtaa agctgccgct 180
gctgcttggt aa 192
<210> 174
<211> 50
<212> PRT
<213> 短杆菌GI-9
<400> 174
Met Ala Cys Gln Cys Pro Asp Ala Ile Ser Gly Trp Thr His Thr Asp
1 5 10 15
Tyr Gln Cys His Gly Leu Glu Asn Lys Met Tyr Arg His Val Tyr Ala
20 25 30
Ile Cys Met Asn Gly Thr Gln Val Tyr Cys Arg Thr Glu Trp Gly Ser
35 40 45
Ser Cys
50
<210> 175
<211> 153
<212> DNA
<213> 短杆菌GI-9
<400> 175
atggcttgcc aatgtccaga tgcgatctca ggttggacgc atacagatta ccagtgtcac 60
ggtttggaga ataaaatgta tagacatgtt tatgcaattt gcatgaacgg tactcaagta 120
tattgcagaa cagagtgggg tagcagctgc tag 153
<210> 176
<211> 57
<212> PRT
<213> 假肠膜明串珠菌
<400> 176
Met Asn Lys Glu Tyr Asn Ser Ile Ser Asn Phe Lys Lys Ile Thr Asn
1 5 10 15
Lys Asp Leu Gln Asn Ile Asn Gly Gly Phe Ile Gly Arg Ala Ile Gly
20 25 30
Asp Phe Val Tyr Phe Gly Ala Lys Gly Leu Arg Glu Ser Gly Lys Leu
35 40 45
Leu Asn Tyr Tyr Tyr Lys His Lys His
50 55
<210> 177
<211> 174
<212> DNA
<213> 假肠膜明串珠菌
<400> 177
atgaataaag aatataatag cattagcaat tttaaaaaaa ttactaataa agacttgcaa 60
aacataaatg gtggatttat tggtagggca ataggtgact ttgtgtactt tggagcgaag 120
ggactaagag aatctggtaa actacttaat tattactata agcataagca ttga 174
<210> 178
<211> 53
<212> PRT
<213> 假肠膜明串珠菌
<400> 178
Met Lys Asn Gln Leu Met Ser Phe Glu Val Ile Ser Glu Lys Glu Leu
1 5 10 15
Ser Thr Val Gln Gly Gly Lys Gly Leu Gly Lys Leu Ile Gly Ile Asp
20 25 30
Trp Leu Leu Gly Gln Ala Lys Asp Ala Val Lys Gln Tyr Lys Lys Asp
35 40 45
Tyr Lys Arg Trp His
50
<210> 179
<211> 162
<212> DNA
<213> 假肠膜明串珠菌
<400> 179
atgaaaaatc agttaatgtc tttcgaagtg atatcagaaa aagaattgtc cacggtacaa 60
ggtggcaaag gcttaggtaa actcatagga attgattggc ttttgggtca agctaaggac 120
gctgttaaac agtacaagaa ggattacaaa cgttggcact aa 162
<210> 180
<211> 61
<212> PRT
<213> 串珠菌
<400> 180
Met Met Asn Met Lys Pro Thr Glu Ser Tyr Glu Gln Leu Asp Asn Ser
1 5 10 15
Ala Leu Glu Gln Val Val Gly Gly Lys Tyr Tyr Gly Asn Gly Val His
20 25 30
Cys Thr Lys Ser Gly Cys Ser Val Asn Trp Gly Glu Ala Phe Ser Ala
35 40 45
Gly Val His Arg Leu Ala Asn Gly Gly Asn Gly Phe Trp
50 55 60
<210> 181
<211> 186
<212> DNA
<213> 串珠菌
<400> 181
atgatgaaca tgaaacctac ggaaagctat gagcaattgg ataatagtgc tctcgaacaa 60
gtcgtaggag gtaagtatta tggtaacgga gttcattgca caaaaagtgg ttgttctgta 120
aactggggag aagccttttc agctggagta catcgtttag caaatggtgg aaatggtttc 180
tggtaa 186
<210> 182
<211> 61
<212> PRT
<213> 明串珠菌
<400> 182
Met Asn Asn Met Lys Ser Ala Asp Asn Tyr Gln Gln Leu Asp Asn Asn
1 5 10 15
Ala Leu Glu Gln Val Val Gly Gly Lys Tyr Tyr Gly Asn Gly Val His
20 25 30
Cys Thr Lys Ser Gly Cys Ser Val Asn Trp Gly Glu Ala Phe Ser Ala
35 40 45
Gly Val His Arg Leu Ala Asn Gly Gly Asn Gly Phe Trp
50 55 60
<210> 183
<211> 186
<212> DNA
<213> 明串珠菌
<400> 183
atgaataaca tgaaatctgc ggataattat cagcaattgg ataataatgc tctcgaacaa 60
gtcgtaggag gtaagtatta tggtaacgga gttcattgca caaaaagtgg ttgttctgta 120
aactggggag aagccttttc agctggagta catcgtttag caaatggtgg aaatggtttc 180
tggtaa 186
<210> 184
<211> 63
<212> PRT
<213> 肠膜明串珠菌
<400> 184
Met Phe Leu Val Asn Gln Leu Gly Ile Ser Lys Ser Leu Ala Asn Thr
1 5 10 15
Ile Leu Gly Ala Ile Ala Val Gly Asn Leu Ala Ser Trp Leu Leu Ala
20 25 30
Leu Val Pro Gly Pro Gly Trp Ala Thr Lys Ala Ala Leu Ala Thr Ala
35 40 45
Glu Thr Ile Val Lys His Glu Gly Lys Ala Ala Ala Ile Ala Trp
50 55 60
<210> 185
<211> 192
<212> DNA
<213> 肠膜明串珠菌
<400> 185
atgttcttgg taaatcagtt agggatttca aaatcgttag ctaatactat tcttggtgca 60
attgctgttg gtaatttggc cagttggtta ttagctttgg ttcctggtcc gggttgggca 120
acaaaagcag cacttgcgac agctgaaaca attgtgaagc atgaaggaaa agcagctgct 180
attgcgtggt aa 192
<210> 186
<211> 74
<212> PRT
<213> 地衣芽孢杆菌
<400> 186
Met Ser Lys Lys Glu Met Ile Leu Ser Trp Lys Asn Pro Met Tyr Arg
1 5 10 15
Thr Glu Ser Ser Tyr His Pro Ala Gly Asn Ile Leu Lys Glu Leu Gln
20 25 30
Glu Glu Glu Gln His Ser Ile Ala Gly Gly Thr Ile Thr Leu Ser Thr
35 40 45
Cys Ala Ile Leu Ser Lys Pro Leu Gly Asn Asn Gly Tyr Leu Cys Thr
50 55 60
Val Thr Lys Glu Cys Met Pro Ser Cys Asn
65 70
<210> 187
<211> 225
<212> DNA
<213> 地衣芽孢杆菌
<400> 187
atgtcaaaaa aggaaatgat tctttcatgg aaaaatccta tgtatcgcac tgaatcttct 60
tatcatccag cagggaacat ccttaaagaa ctccaggaag aggaacagca cagcatcgcc 120
ggaggcacaa tcacgctcag cacttgtgcc atcttgagca agccgttagg aaataacgga 180
tacctgtgta cagtgacaaa agaatgcatg ccaagctgta actaa 225
<210> 188
<211> 266
<212> PRT
<213> 亚麻短杆菌
<400> 188
Met Asn Asn Leu Tyr Arg Glu Leu Ala Pro Ile Pro Gly Pro Ala Trp
1 5 10 15
Ala Glu Ile Glu Glu Glu Ala Arg Arg Thr Phe Lys Arg Asn Ile Ala
20 25 30
Gly Arg Arg Ile Val Asp Val Ala Gly Pro Thr Gly Phe Glu Thr Ser
35 40 45
Ala Val Thr Thr Gly His Ile Arg Asp Val Gln Ser Glu Thr Ser Gly
50 55 60
Leu Gln Val Lys Gln Arg Ile Val Gln Glu Tyr Ile Glu Leu Arg Thr
65 70 75 80
Pro Phe Thr Val Thr Arg Gln Ala Ile Asp Asp Val Ala Arg Gly Ser
85 90 95
Gly Asp Ser Asp Trp Gln Pro Val Lys Asp Ala Ala Thr Thr Ile Ala
100 105 110
Met Ala Glu Asp Arg Ala Ile Leu His Gly Leu Asp Ala Ala Gly Ile
115 120 125
Gly Gly Ile Val Pro Gly Ser Ser Asn Ala Ala Val Ala Ile Pro Asp
130 135 140
Ala Val Glu Asp Phe Ala Asp Ala Val Ala Gln Ala Leu Ser Val Leu
145 150 155 160
Arg Thr Val Gly Val Asp Gly Pro Tyr Ser Leu Leu Leu Ser Ser Ala
165 170 175
Glu Tyr Thr Lys Val Ser Glu Ser Thr Asp His Gly Tyr Pro Ile Arg
180 185 190
Glu His Leu Ser Arg Gln Leu Gly Ala Gly Glu Ile Ile Trp Ala Pro
195 200 205
Ala Leu Glu Gly Ala Leu Leu Val Ser Thr Arg Gly Gly Asp Tyr Glu
210 215 220
Leu His Leu Gly Gln Asp Leu Ser Ile Gly Tyr Tyr Ser His Asp Ser
225 230 235 240
Glu Thr Val Glu Leu Tyr Leu Gln Glu Thr Phe Gly Phe Leu Ala Leu
245 250 255
Thr Asp Glu Ser Ser Val Pro Leu Ser Leu
260 265
<210> 189
<211> 801
<212> DNA
<213> 亚麻短杆菌
<400> 189
gtgaataacc tctatcgcga gcttgccccc atccccggcc cggcctgggc ggagatcgag 60
gaggaggctc gacggacatt caaacgcaat atcgccggcc gccggatcgt cgatgtcgca 120
gggcccacgg gcttcgagac ctccgcggtg accactggcc acatccgaga cgtccagtcg 180
gagacgagcg gactgcaggt taagcagcgc atcgtgcagg aatacatcga gctgcggacc 240
ccattcaccg tgactcggca ggccatcgat gacgtggccc gcgggtccgg tgactcggac 300
tggcagcccg tcaaggatgc ggccacgacg atcgcgatgg ctgaagatcg ggccattctc 360
cacgggctcg atgcggccgg gatcggcgga atcgttcccg gcagctcgaa tgccgcagtg 420
gccatccccg acgccgtcga ggacttcgcg gacgccgtcg cccaggcgct gagtgtgctg 480
cgcacggtgg gagtcgacgg gccctacagc ctgttgctct cctccgcgga gtacaccaag 540
gtctccgagt ccaccgacca cggctacccg atccgcgagc acctctcccg gcagctcggc 600
gccggagaga tcatctgggc gcccgcgctc gaaggggcgc tgctcgtctc cacgcgcggg 660
ggtgactacg agctccacct cggccaggac ctgtcgatcg gttactacag ccacgacagc 720
gagaccgtcg aactctatct gcaggagacc ttcggattcc tcgcgctgac cgacgaatcc 780
agtgtgcctt tgagcctctg a 801
<210> 190
<211> 71
<212> PRT
<213> 李斯特菌
<400> 190
Met Lys Lys Ala Ala Leu Lys Phe Ile Ile Val Ile Ala Ile Leu Gly
1 5 10 15
Phe Ser Phe Ser Phe Phe Ser Ile Gln Ser Glu Ala Lys Ser Tyr Gly
20 25 30
Asn Gly Val Gln Cys Asn Lys Lys Lys Cys Trp Val Asp Trp Gly Ser
35 40 45
Ala Ile Ser Thr Ile Gly Asn Asn Ser Ala Ala Asn Trp Ala Thr Gly
50 55 60
Gly Ala Ala Gly Trp Lys Ser
65 70
<210> 191
<211> 216
<212> DNA
<213> 李斯特菌
<400> 191
ttgaagaagg cagcgttaaa atttattatt gttattgcta ttctaggttt cagtttttct 60
ttctttagca tacaatctga agctaaatct tatggaaatg gagttcagtg taataagaaa 120
aaatgttggg tagattgggg tagtgctata agtactattg gaaataattc tgcagcgaat 180
tgggctacag gtggagcagc tggttggaaa agctga 216
<210> 192
<211> 68
<212> PRT
<213> 芽孢杆菌
<400> 192
Met Ser Gln Glu Ala Ile Ile Arg Ser Trp Lys Asp Pro Phe Ser Arg
1 5 10 15
Glu Asn Ser Thr Gln Asn Pro Ala Gly Asn Pro Phe Ser Glu Leu Lys
20 25 30
Glu Ala Gln Met Asp Lys Leu Val Gly Ala Gly Asp Met Glu Ala Ala
35 40 45
Cys Thr Phe Thr Leu Pro Gly Gly Gly Gly Val Cys Thr Leu Thr Ser
50 55 60
Glu Cys Ile Cys
65
<210> 193
<211> 207
<212> DNA
<213> 芽孢杆菌
<400> 193
atgagtcaag aagctatcat tcgttcatgg aaagatcctt tttcccgtga aaattctaca 60
caaaatccag ctggtaaccc attcagtgag ctgaaagaag cacaaatgga taagttagta 120
ggtgcgggag acatggaagc agcatgtact tttacattgc ctggtggcgg cggtgtttgt 180
actctaactt ctgaatgtat ttgttaa 207
<210> 194
<211> 61
<212> PRT
<213> 肠膜明串珠菌
<400> 194
Met Thr Asn Met Lys Ser Val Glu Ala Tyr Gln Gln Leu Asp Asn Gln
1 5 10 15
Asn Leu Lys Lys Val Val Gly Gly Lys Tyr Tyr Gly Asn Gly Val His
20 25 30
Cys Thr Lys Ser Gly Cys Ser Val Asn Trp Gly Glu Ala Ala Ser Ala
35 40 45
Gly Ile His Arg Leu Ala Asn Gly Gly Asn Gly Phe Trp
50 55 60
<210> 195
<211> 186
<212> DNA
<213> 肠膜明串珠菌
<400> 195
atgacgaata tgaagtctgt ggaagcatat cagcaattag ataaccagaa tctcaagaaa 60
gttgttggtg gaaagtatta tgggaatggt gttcactgta caaaaagtgg atgctctgtt 120
aactggggag aagctgcctc agctggcata catcgtttgg ccaatggtgg aaatggattt 180
tggtaa 186
<210> 196
<211> 68
<212> PRT
<213> 密歇根棒状杆菌密歇根亚种
<400> 196
Met Asn Asp Ile Leu Glu Thr Glu Thr Pro Val Met Val Ser Pro Arg
1 5 10 15
Trp Asp Met Leu Leu Asp Ala Gly Glu Asp Thr Ser Pro Ser Val Gln
20 25 30
Thr Gln Ile Asp Ala Glu Phe Arg Arg Val Val Ser Pro Tyr Met Ser
35 40 45
Ser Ser Gly Trp Leu Cys Thr Leu Thr Ile Glu Cys Gly Thr Ile Ile
50 55 60
Cys Ala Cys Arg
65
<210> 197
<211> 207
<212> DNA
<213> 密歇根棒状杆菌密歇根亚种
<400> 197
atgaacgaca tcctcgagac ggagaccccc gtcatggtca gcccccggtg ggacatgctg 60
ctcgacgcgg gcgaggacac cagcccgtcc gtccagaccc agatcgacgc ggagttccgt 120
cgcgtcgtga gcccgtacat gtccagcagc ggctggctct gcacgctcac catcgaatgt 180
ggcaccatca tctgcgcgtg tcgctga 207
<210> 198
<211> 69
<212> PRT
<213> 大肠杆菌
<400> 198
Met Glu Leu Lys Ala Ser Glu Phe Gly Val Val Leu Ser Val Asp Ala
1 5 10 15
Leu Lys Leu Ser Arg Gln Ser Pro Leu Gly Val Gly Ile Gly Gly Gly
20 25 30
Gly Gly Gly Gly Gly Gly Gly Ser Cys Gly Gly Gln Gly Gly Gly Cys
35 40 45
Gly Gly Cys Ser Asn Gly Cys Ser Gly Gly Asn Gly Gly Ser Gly Gly
50 55 60
Ser Gly Ser His Ile
65
<210> 199
<211> 207
<212> DNA
<213> 大肠杆菌
<400> 199
atggaattaa aagcgagtga atttggtgta gttttgtccg ttgatgctct taaattatca 60
cgccagtctc cattaggtgt tggcattggt ggtggtggcg gcggcggcgg cggcggtagc 120
tgcggtggtc aaggtggcgg ttgtggtggt tgcagcaacg gttgtagtgg tggaaacggt 180
ggcagcggcg gaagtggttc acatatc 207
<210> 200
<211> 7
<212> PRT
<213> 大肠杆菌
<400> 200
Met Arg Thr Gly Asn Ala Asn
1 5
<210> 201
<211> 24
<212> DNA
<213> 大肠杆菌
<400> 201
atgcgtactg gtaatgcaaa ctaa 24
<210> 202
<211> 99
<212> PRT
<213> 肺炎克雷伯菌
<400> 202
Met Arg Glu Ile Ser Gln Lys Asp Leu Asn Leu Ala Phe Gly Ala Gly
1 5 10 15
Glu Thr Asp Pro Asn Thr Gln Leu Leu Asn Asp Leu Gly Asn Asn Met
20 25 30
Ala Trp Gly Ala Ala Leu Gly Ala Pro Gly Gly Leu Gly Ser Ala Ala
35 40 45
Leu Gly Ala Ala Gly Gly Ala Leu Gln Thr Val Gly Gln Gly Leu Ile
50 55 60
Asp His Gly Pro Val Asn Val Pro Ile Pro Val Leu Ile Gly Pro Ser
65 70 75 80
Trp Asn Gly Ser Gly Ser Gly Tyr Asn Ser Ala Thr Ser Ser Ser Gly
85 90 95
Ser Gly Ser
<210> 203
<211> 300
<212> DNA
<213> 肺炎克雷伯菌
<400> 203
atgagagaaa ttagtcaaaa ggacttaaat cttgcttttg gtgcaggaga gaccgatcca 60
aatactcaac ttctaaacga ccttggaaat aatatggcat ggggtgctgc tcttggcgct 120
cctggcggat taggatcagc agctttgggg gccgcgggag gtgcattaca aactgtaggg 180
caaggattaa ttgaccatgg tcctgtaaat gtccccatcc ctgtactcat cgggccaagc 240
tggaatggta gcggtagtgg ttataacagc gcaacatcca gttccggtag tggtagttaa 300
<210> 204
<211> 75
<212> PRT
<213> 大肠杆菌
<400> 204
Met Arg Glu Ile Thr Glu Ser Gln Leu Arg Tyr Ile Ser Gly Ala Gly
1 5 10 15
Gly Ala Pro Ala Thr Ser Ala Asn Ala Ala Gly Ala Ala Ala Ile Val
20 25 30
Gly Ala Leu Ala Gly Ile Pro Gly Gly Pro Leu Gly Val Val Val Gly
35 40 45
Ala Val Ser Ala Gly Leu Thr Thr Ala Ile Gly Ser Thr Val Gly Ser
50 55 60
Gly Ser Ala Ser Ser Ser Ala Gly Gly Gly Ser
65 70 75
<210> 205
<211> 228
<212> DNA
<213> 大肠杆菌
<400> 205
atgcgagaaa taacagaatc acagttaaga tatatttccg gggcgggagg tgcgccagcg 60
acttcagcta atgccgcagg tgctgcagct attgttggag ctctcgccgg aatacctggt 120
ggtccacttg gggttgtagt tggagccgta tctgccggtt tgacaacagc aattggctcg 180
accgtgggaa gtggtagtgc cagttcttct gctggtggcg gtagctaa 228
<210> 206
<211> 58
<212> PRT
<213> 大肠杆菌
<400> 206
Met Ile Lys His Phe His Phe Asn Lys Leu Ser Ser Gly Lys Lys Asn
1 5 10 15
Asn Val Pro Ser Pro Ala Lys Gly Val Ile Gln Ile Lys Lys Ser Ala
20 25 30
Ser Gln Leu Thr Lys Gly Gly Ala Gly His Val Pro Glu Tyr Phe Val
35 40 45
Gly Ile Gly Thr Pro Ile Ser Phe Tyr Gly
50 55
<210> 207
<211> 177
<212> DNA
<213> 大肠杆菌
<400> 207
atgattaagc attttcattt taataaactg tcttctggta aaaaaaataa tgttccatct 60
cctgcaaagg gggttataca aataaaaaaa tcagcatcgc aactcacaaa aggtggtgca 120
ggacatgtgc ctgagtattt tgtggggatt ggtacaccta tatctttcta tggctga 177
<210> 208
<211> 90
<212> PRT
<213> 大肠杆菌
<400> 208
Met Tyr Met Arg Glu Leu Asp Arg Glu Glu Leu Asn Cys Val Gly Gly
1 5 10 15
Ala Gly Asp Pro Leu Ala Asp Pro Asn Ser Gln Ile Val Arg Gln Ile
20 25 30
Met Ser Asn Ala Ala Trp Gly Pro Pro Leu Val Pro Glu Arg Phe Arg
35 40 45
Gly Met Ala Val Gly Ala Ala Gly Gly Val Thr Gln Thr Val Leu Gln
50 55 60
Gly Ala Ala Ala His Met Pro Val Asn Val Pro Ile Pro Lys Val Pro
65 70 75 80
Met Gly Pro Ser Trp Asn Gly Ser Lys Gly
85 90
<210> 209
<211> 273
<212> DNA
<213> 大肠杆菌
<400> 209
atgtatatga gagagttaga tagagaggaa ttaaattgcg ttggtggggc tggagatccg 60
cttgcagatc ctaattccca aattgtaaga cagataatgt ctaatgcggc atggggcccg 120
cctttggtgc cagagcggtt taggggaatg gctgttggag ccgcaggtgg ggttacgcag 180
acagttcttc aaggagcagc agctcatatg ccggttaatg tccctatacc taaagttccg 240
atgggaccct catggaacgg aagtaaagga taa 273
<210> 210
<211> 50
<212> PRT
<213> 蒙氏肠球菌
<400> 210
Met Ser Gln Val Val Gly Gly Lys Tyr Tyr Gly Asn Gly Val Ser Cys
1 5 10 15
Asn Lys Lys Gly Cys Ser Val Asp Trp Gly Lys Ala Ile Gly Ile Ile
20 25 30
Gly Asn Asn Ser Ala Ala Asn Leu Ala Thr Gly Gly Ala Ala Gly Trp
35 40 45
Lys Ser
50
<210> 211
<211> 153
<212> DNA
<213> 蒙氏肠球菌
<400> 211
atgtcacagg tagtaggtgg aaaatactac ggtaatggag tctcatgtaa taaaaaaggg 60
tgcagtgttg attggggaaa agcgattggc attattggaa ataattctgc tgcgaattta 120
gctactggtg gagcagctgg ttggaaaagt taa 153
<210> 212
<211> 58
<212> PRT
<213> 蒙氏肠球菌
<400> 212
Met Lys Lys Leu Thr Ser Lys Glu Met Ala Gln Val Val Gly Gly Lys
1 5 10 15
Tyr Tyr Gly Asn Gly Leu Ser Cys Asn Lys Lys Gly Cys Ser Val Asp
20 25 30
Trp Gly Lys Ala Ile Gly Ile Ile Gly Asn Asn Ser Ala Ala Asn Leu
35 40 45
Ala Thr Gly Gly Ala Ala Gly Trp Lys Ser
50 55
<210> 213
<211> 177
<212> DNA
<213> 蒙氏肠球菌
<400> 213
ttgaagaaat taacatcaaa agaaatggca caagtagtag gtgggaaata ctacggtaat 60
ggattatcat gtaataaaaa agggtgcagt gttgattggg gaaaagctat tggcattatt 120
ggaaataatt ctgctgcgaa tttagctact ggtggagcag ctggttggaa aagttaa 177
<210> 214
<211> 63
<212> PRT
<213> 变形链球菌
<400> 214
Met Ser Asn Thr Gln Leu Leu Glu Val Leu Gly Thr Glu Thr Phe Asp
1 5 10 15
Val Gln Glu Asp Leu Phe Ala Phe Asp Thr Thr Asp Thr Thr Ile Val
20 25 30
Ala Ser Asn Asp Asp Pro Asp Thr Arg Phe Lys Ser Trp Ser Leu Cys
35 40 45
Thr Pro Gly Cys Ala Arg Thr Gly Ser Phe Asn Ser Tyr Cys Cys
50 55 60
<210> 215
<211> 192
<212> DNA
<213> 变形链球菌
<400> 215
atgtcaaaca cacaattatt agaagtcctt ggtactgaaa cttttgatgt tcaagaagat 60
ctctttgctt ttgatacaac agatactact attgtggcaa gcaacgacga tccagatact 120
cgtttcaaaa gttggagcct ttgtacgcct ggttgtgcaa ggacaggtag tttcaatagt 180
tactgttgct ga 192
<210> 216
<211> 53
<212> PRT
<213> 变形链球菌
<400> 216
Met Asn Lys Leu Asn Ser Asn Ala Val Val Ser Leu Asn Glu Val Ser
1 5 10 15
Asp Ser Glu Leu Asp Thr Ile Leu Gly Gly Asn Arg Trp Trp Gln Gly
20 25 30
Val Val Pro Thr Val Ser Tyr Glu Cys Arg Met Asn Ser Trp Gln His
35 40 45
Val Phe Thr Cys Cys
50
<210> 217
<211> 162
<212> DNA
<213> 变形链球菌
<400> 217
atgaacaagt taaacagtaa cgcagtagtt tctttgaatg aagtttcaga ttctgaattg 60
gatactattt tgggtggtaa tcgttggtgg caaggtgttg tgccaacggt ctcatatgag 120
tgtcgcatga attcatggca acatgttttc acttgctgtt aa 162
<210> 218
<211> 57
<212> PRT
<213> 乳酸乳球菌乳酸亚种
<400> 218
Met Ser Thr Lys Asp Phe Asn Leu Asp Leu Val Ser Val Ser Lys Lys
1 5 10 15
Asp Ser Gly Ala Ser Pro Arg Ile Thr Ser Ile Ser Leu Cys Thr Pro
20 25 30
Gly Cys Lys Thr Gly Ala Leu Met Gly Cys Asn Met Lys Thr Ala Thr
35 40 45
Cys His Cys Ser Ile His Val Ser Lys
50 55
<210> 219
<211> 174
<212> DNA
<213> 乳酸乳球菌乳酸亚种
<400> 219
atgagtacaa aagattttaa cttggatttg gtatctgttt cgaagaaaga ttcaggtgca 60
tcaccacgca ttacaagtat ttcgctatgt acacccggtt gtaaaacagg agctctgatg 120
ggttgtaaca tgaaaacagc aacttgtcat tgtagtattc acgtaagcaa ataa 174
<210> 220
<211> 57
<212> PRT
<213> 乳酸乳球菌
<400> 220
Met Ser Thr Lys Asp Phe Asn Leu Asp Leu Val Ser Val Ser Lys Lys
1 5 10 15
Asp Ser Gly Ala Ser Pro Arg Ile Thr Ser Ile Ser Leu Cys Thr Pro
20 25 30
Gly Cys Lys Thr Gly Ala Leu Met Gly Cys Asn Met Lys Thr Ala Thr
35 40 45
Cys Asn Cys Ser Val His Val Ser Lys
50 55
<210> 221
<211> 171
<212> DNA
<213> 乳酸乳球菌
<400> 221
atgagtacaa aagatttcaa cttggatttg gtatctgttt cgaagaaaga ttcaggtgca 60
tcaccacgca ttacaagtat ttcgctatgt acacccggtt gtaaaacagg agctctgatg 120
ggttgtaaca tgaaaacagc aacttgtaat tgtagcgttc acgtaagcaa a 171
<210> 222
<211> 57
<212> PRT
<213> 乳酸乳球菌
<400> 222
Met Ser Thr Lys Asp Phe Asn Leu Asp Leu Val Ser Val Ser Lys Thr
1 5 10 15
Asp Ser Gly Ala Ser Thr Arg Ile Thr Ser Ile Ser Leu Cys Thr Pro
20 25 30
Gly Cys Lys Thr Gly Val Leu Met Gly Cys Asn Leu Lys Thr Ala Thr
35 40 45
Cys Asn Cys Ser Val His Val Ser Lys
50 55
<210> 223
<211> 174
<212> DNA
<213> 乳酸乳球菌
<400> 223
atgagtacaa aagatttcaa cttagatttg gtatctgttt caaaaacaga ttctggcgct 60
tcaacacgta ttaccagcat ttcgctttgt acaccaggtt gtaaaacagg tgttctgatg 120
ggatgtaacc tgaaaacagc aacttgtaat tgtagcgttc acgtaagcaa ataa 174
<210> 224
<211> 55
<212> PRT
<213> ***链球菌
<400> 224
Met Asn Asn Glu Asp Phe Asn Leu Asp Leu Ile Lys Ile Ser Lys Glu
1 5 10 15
Asn Asn Ser Gly Ala Ser Pro Arg Ile Thr Ser Lys Ser Leu Cys Thr
20 25 30
Pro Gly Cys Lys Thr Gly Ile Leu Met Thr Cys Pro Leu Lys Thr Ala
35 40 45
Thr Cys Gly Cys His Phe Gly
50 55
<210> 225
<211> 168
<212> DNA
<213> ***链球菌
<400> 225
atgaacaatg aagattttaa tttggatctc atcaaaatct caaaggaaaa caactcagga 60
gcttcacctc gaataactag taaatcatta tgtactcctg gatgtaagac gggtattttg 120
atgacttgtc cactaaaaac tgcaacctgt ggttgtcatt ttggataa 168
<210> 226
<211> 57
<212> PRT
<213> 乳酸乳球菌乳酸亚种
<400> 226
Met Ser Thr Lys Asp Phe Asn Leu Asp Leu Val Ser Val Ser Lys Lys
1 5 10 15
Asp Ser Gly Ala Ser Pro Arg Ile Thr Ser Ile Ser Leu Cys Thr Pro
20 25 30
Gly Cys Lys Thr Gly Ala Leu Met Gly Cys Asn Met Lys Thr Ala Thr
35 40 45
Cys Asn Cys Ser Ile His Val Ser Lys
50 55
<210> 227
<211> 174
<212> DNA
<213> 乳酸乳球菌乳酸亚种
<400> 227
atgagtacaa aagattttaa cttggatttg gtatctgttt cgaagaaaga ttcaggtgca 60
tcaccacgca ttacaagtat ttcgctatgt acacccggtt gtaaaacagg agctctgatg 120
ggttgtaaca tgaaaacagc aacttgtaat tgtagtattc acgtaagcaa ataa 174
<210> 228
<211> 57
<212> PRT
<213> 沃氏葡萄球菌
<400> 228
Met Glu Asn Ser Lys Val Met Lys Asp Ile Glu Val Ala Asn Leu Leu
1 5 10 15
Glu Glu Val Gln Glu Asp Glu Leu Asn Glu Val Leu Gly Ala Lys Lys
20 25 30
Lys Ser Gly Val Ile Pro Thr Val Ser His Asp Cys His Met Asn Ser
35 40 45
Phe Gln Phe Val Phe Thr Cys Cys Ser
50 55
<210> 229
<211> 174
<212> DNA
<213> 沃氏葡萄球菌
<400> 229
atggaaaatt ctaaagttat gaaggacatt gaagtagcaa atttattaga agaggttcaa 60
gaagatgaat tgaatgaagt cttaggagct aagaaaaagt caggagtaat cccaactgtg 120
tcacacgatt gccatatgaa ttctttccaa tttgtattta cttgttgttc ataa 174
<210> 230
<211> 58
<212> PRT
<213> 多粘类芽孢杆菌
<400> 230
Met Ala Glu Asn Leu Phe Asp Leu Asp Ile Gln Val Asn Lys Ser Gln
1 5 10 15
Gly Ser Val Glu Pro Gln Val Leu Ser Ile Val Ala Cys Ser Ser Gly
20 25 30
Cys Gly Ser Gly Lys Thr Ala Ala Ser Cys Val Glu Thr Cys Gly Asn
35 40 45
Arg Cys Phe Thr Asn Val Gly Ser Leu Cys
50 55
<210> 231
<211> 177
<212> DNA
<213> 多粘类芽孢杆菌
<400> 231
atggctgaaa acttatttga tctggacatt caagtaaaca aatctcaagg ttctgtagag 60
cctcaggttc tgagcattgt tgcatgttct agcggatgtg gtagcggtaa aacagctgcc 120
agttgtgttg aaacttgtgg caaccggtgc tttactaacg ttggttcact ctgctaa 177
<210> 232
<211> 62
<212> PRT
<213> 乳酸片球菌
<400> 232
Met Lys Lys Ile Glu Lys Leu Thr Glu Lys Glu Met Ala Asn Ile Ile
1 5 10 15
Gly Gly Lys Tyr Tyr Gly Asn Gly Val Thr Cys Gly Lys His Ser Cys
20 25 30
Ser Val Asp Trp Gly Lys Ala Thr Thr Cys Ile Ile Asn Asn Gly Ala
35 40 45
Met Ala Trp Ala Thr Gly Gly His Gln Gly Asn His Lys Cys
50 55 60
<210> 233
<211> 189
<212> DNA
<213> 乳酸片球菌
<400> 233
atgaaaaaaa ttgaaaaatt aactgaaaaa gaaatggcca atatcattgg tggtaaatac 60
tacggtaatg gggttacttg tggcaaacat tcctgctctg ttgactgggg taaggctacc 120
acttgcataa tcaataatgg agctatggca tgggctactg gtggacatca aggtaatcat 180
aaatgctag 189
<210> 234
<211> 60
<212> PRT
<213> 戊糖片球菌
<400> 234
Met Thr Glu Ile Lys Val Leu Asn Asp Lys Glu Leu Lys Asn Val Val
1 5 10 15
Gly Gly Lys Tyr Tyr Gly Asn Gly Val His Cys Gly Lys Lys Thr Cys
20 25 30
Tyr Val Asp Trp Gly Gln Ala Thr Ala Ser Ile Gly Lys Ile Ile Val
35 40 45
Asn Gly Trp Thr Gln His Gly Pro Trp Ala His Arg
50 55 60
<210> 235
<211> 183
<212> DNA
<213> 戊糖片球菌
<400> 235
atgactgaaa ttaaagtact aaacgataag gaactaaaaa atgtcgtagg aggaaagtat 60
tacggtaacg gagtgcattg tggtaaaaag acttgctatg tggactgggg acaagctaca 120
gctagcattg gaaaaattat agtgaacgga tggacacaac acgggccttg ggcacataga 180
taa 183
<210> 236
<211> 60
<212> PRT
<213> 表皮葡萄球菌
<400> 236
Met Lys Asn Asn Lys Asn Leu Phe Asp Leu Glu Ile Lys Lys Glu Thr
1 5 10 15
Ser Gln Asn Thr Asp Glu Leu Glu Pro Gln Thr Ala Gly Pro Ala Ile
20 25 30
Arg Ala Ser Val Lys Gln Cys Gln Lys Thr Leu Lys Ala Thr Arg Leu
35 40 45
Phe Thr Val Ser Cys Lys Gly Lys Asn Gly Cys Lys
50 55 60
<210> 237
<211> 183
<212> DNA
<213> 表皮葡萄球菌
<400> 237
atgaaaaata acaaaaattt atttgattta gaaattaaaa aagaaacaag tcaaaacact 60
gatgaacttg aacctcaaac tgctggacca gcgattagag cttctgtgaa acaatgtcag 120
aaaactttga aagctacgcg tttatttaca gtgtcttgca aaggaaaaaa cggatgtaaa 180
tag 183
<210> 238
<211> 62
<212> PRT
<213> 麦芽香肉杆菌
<400> 238
Met Lys Thr Val Lys Glu Leu Ser Val Lys Glu Met Gln Leu Thr Thr
1 5 10 15
Gly Gly Lys Tyr Tyr Gly Asn Gly Val Ser Cys Asn Lys Asn Gly Cys
20 25 30
Thr Val Asp Trp Ser Lys Ala Ile Gly Ile Ile Gly Asn Asn Ala Ala
35 40 45
Ala Asn Leu Thr Thr Gly Gly Ala Ala Gly Trp Asn Lys Gly
50 55 60
<210> 239
<211> 189
<212> DNA
<213> 麦芽香肉杆菌
<400> 239
atgaaaactg ttaaagaact tagcgttaaa gaaatgcaac taactacagg aggtaagtat 60
tacggaaatg gcgtttcctg taataaaaat ggttgtactg tagattggag caaagctatt 120
gggattatag gaaacaatgc agcagcaaat ttgactacag gtggagccgc tggttggaac 180
aaaggataa 189
<210> 240
<211> 69
<212> PRT
<213> 植物乳杆菌
<220>
<221> 变体
<222> (40)..(40)
<223> Xaa = 任何氨基酸
<400> 240
Met Tyr Lys Glu Leu Thr Val Asp Glu Leu Ala Leu Ile Asp Gly Gly
1 5 10 15
Lys Lys Lys Lys Lys Lys Val Ala Cys Thr Trp Gly Asn Ala Ala Thr
20 25 30
Ala Ala Ala Ser Gly Ala Val Xaa Gly Ile Leu Gly Gly Pro Thr Gly
35 40 45
Ala Leu Ala Gly Ala Ile Trp Gly Val Ser Gln Cys Ala Ser Asn Asn
50 55 60
Leu His Gly Met His
65
<210> 241
<211> 210
<212> DNA
<213> 植物乳杆菌
<220>
<221> misc_feature
<222> (119)..(119)
<223> n = a, c, t或g
<400> 241
atgtataaag aattaacagt tgatgaatta gcattgattg atggaggaaa aaagaagaag 60
aaaaaagtag cttgtacttg gggaaatgca gcaacagccg ctgcttctgg tgcagttang 120
ggtattcttg gtgggcctac tggtgcactg gctggagcta tctggggcgt ttcacaatgc 180
gcgtctaaca acttacacgg catgcactaa 210
<210> 242
<211> 56
<212> PRT
<213> 植物乳杆菌
<400> 242
Met Met Lys Lys Ile Glu Lys Leu Thr Glu Lys Glu Met Ala Asn Ile
1 5 10 15
Ile Gly Gly Lys Tyr Tyr Gly Asn Gly Val Thr Cys Gly Lys His Ser
20 25 30
Cys Ser Val Asn Trp Gly Gln Ala Phe Ser Cys Ser Val Ser His Leu
35 40 45
Ala Asn Phe Gly His Gly Lys Cys
50 55
<210> 243
<211> 171
<212> DNA
<213> 植物乳杆菌
<400> 243
atgatgaaaa aaattgaaaa attaactgaa aaagaaatgg ccaatatcat tggtggtaaa 60
tactatggta atggggttac ttgtggtaaa cattcctgct ctgttaactg gggccaagca 120
ttttcttgta gtgtgtcaca tttagctaac ttcggtcatg gaaagtgcta a 171
<210> 244
<211> 64
<212> PRT
<213> 植物乳杆菌
<400> 244
Met Ser Lys Leu Val Lys Thr Leu Thr Val Asp Glu Ile Ser Lys Ile
1 5 10 15
Gln Thr Asn Gly Gly Lys Pro Ala Trp Cys Trp Tyr Thr Leu Ala Met
20 25 30
Cys Gly Ala Gly Tyr Asp Ser Gly Thr Cys Asp Tyr Met Tyr Ser His
35 40 45
Cys Phe Gly Val Lys His Ser Ser Gly Gly Gly Gly Ser Tyr His Cys
50 55 60
<210> 245
<211> 195
<212> DNA
<213> 植物乳杆菌
<400> 245
atgagtaaac tagttaaaac attaactgtc gatgaaatct ctaagattca aaccaatggt 60
ggaaaacctg catggtgttg gtacacattg gcaatgtgcg gtgctggtta tgattcaggc 120
acttgtgatt atatgtattc acactgcttt ggtgtaaaac actctagcgg tggtggcggt 180
agctaccatt gttag 195
<210> 246
<211> 56
<212> PRT
<213> 植物乳杆菌
<400> 246
Met Leu Gln Phe Glu Lys Leu Gln Tyr Ser Arg Leu Pro Gln Lys Lys
1 5 10 15
Leu Ala Lys Ile Ser Gly Gly Phe Asn Arg Gly Gly Tyr Asn Phe Gly
20 25 30
Lys Ser Val Arg His Val Val Asp Ala Ile Gly Ser Val Ala Gly Ile
35 40 45
Arg Gly Ile Leu Lys Ser Ile Arg
50 55
<210> 247
<211> 171
<212> DNA
<213> 植物乳杆菌
<400> 247
atgctacagt ttgagaaatt acaatattcc aggttgccgc aaaaaaagct tgccaaaata 60
tctggtggtt ttaatcgggg cggttataac tttggtaaaa gtgttcgaca tgttgttgat 120
gcaattggtt cagttgcagg cattcgtggt attttgaaaa gtattcgtta a 171
<210> 248
<211> 52
<212> PRT
<213> 植物乳杆菌
<400> 248
Met Lys Lys Phe Leu Val Leu Arg Asp Arg Glu Leu Asn Ala Ile Ser
1 5 10 15
Gly Gly Val Phe His Ala Tyr Ser Ala Arg Gly Val Arg Asn Asn Tyr
20 25 30
Lys Ser Ala Val Gly Pro Ala Asp Trp Val Ile Ser Ala Val Arg Gly
35 40 45
Phe Ile His Gly
50
<210> 249
<211> 159
<212> DNA
<213> 植物乳杆菌
<400> 249
atgaaaaaat ttctagtttt gcgtgaccgt gaattaaatg ctatttcagg tggcgttttc 60
catgcctata gcgcgcgtgg cgttcggaat aattataaaa gtgctgttgg gcctgccgat 120
tgggtcatta gcgctgtccg aggattcatc cacggatag 159
<210> 250
<211> 55
<212> PRT
<213> 植物乳杆菌
<400> 250
Met Thr Val Asn Lys Met Ile Lys Asp Leu Asp Val Val Asp Ala Phe
1 5 10 15
Ala Pro Ile Ser Asn Asn Lys Leu Asn Gly Val Val Gly Gly Gly Ala
20 25 30
Trp Lys Asn Phe Trp Ser Ser Leu Arg Lys Gly Phe Tyr Asp Gly Glu
35 40 45
Ala Gly Arg Ala Ile Arg Arg
50 55
<210> 251
<211> 168
<212> DNA
<213> 植物乳杆菌
<400> 251
atgactgtga acaaaatgat taaggatttg gatgtagtag atgcatttgc acctatttct 60
aataataagt tgaacggggt tgttggggga ggcgcttgga aaaatttctg gtctagttta 120
agaaaaggat tttatgatgg cgaagctggc agagcaatcc gtcgttaa 168
<210> 252
<211> 57
<212> PRT
<213> 植物乳杆菌
<400> 252
Met Lys Ile Lys Leu Thr Val Leu Asn Glu Phe Glu Glu Leu Thr Ala
1 5 10 15
Asp Ala Glu Lys Asn Ile Ser Gly Gly Arg Arg Ser Arg Lys Asn Gly
20 25 30
Ile Gly Tyr Ala Ile Gly Tyr Ala Phe Gly Ala Val Glu Arg Ala Val
35 40 45
Leu Gly Gly Ser Arg Asp Tyr Asn Lys
50 55
<210> 253
<211> 174
<212> DNA
<213> 植物乳杆菌
<400> 253
atgaaaatta aattaactgt tttaaatgaa tttgaagaat taactgctga cgctgaaaag 60
aatatttctg gtggccgtcg gagtcgtaaa aatggaattg gatacgctat tggttatgcg 120
tttggcgcgg ttgaacgggc cgtgcttggt ggttcaaggg attataataa gtga 174
<210> 254
<211> 47
<212> PRT
<213> 植物乳杆菌
<400> 254
Met Asp Lys Phe Glu Lys Ile Ser Thr Ser Asn Leu Glu Lys Ile Ser
1 5 10 15
Gly Gly Asp Leu Thr Thr Lys Leu Trp Ser Ser Trp Gly Tyr Tyr Leu
20 25 30
Gly Lys Lys Ala Arg Trp Asn Leu Lys His Pro Tyr Val Gln Phe
35 40 45
<210> 255
<211> 141
<212> DNA
<213> 植物乳杆菌
<400> 255
atggataaat ttgaaaaaat tagtacatct aacctagaaa agatctctgg cggtgattta 60
acaaccaagt tatggagctc ttggggatat tatcttggca agaaagcacg ttggaattta 120
aagcacccat atgttcaatt t 141
<210> 256
<211> 55
<212> PRT
<213> 植物乳杆菌
<400> 256
Met Asn Asn Leu Asn Lys Phe Ser Thr Leu Gly Lys Ser Ser Leu Ser
1 5 10 15
Gln Ile Glu Gly Gly Ser Val Pro Thr Ser Val Tyr Thr Leu Gly Ile
20 25 30
Lys Ile Leu Trp Ser Ala Tyr Lys His Arg Lys Thr Ile Glu Lys Ser
35 40 45
Phe Asn Lys Gly Phe Tyr His
50 55
<210> 257
<211> 168
<212> DNA
<213> 植物乳杆菌
<400> 257
atgaataact tgaataaatt ttctactcta ggcaagagta gcttgtctca aattgagggc 60
ggatcagtcc caacttcagt atatacgctt ggaattaaaa ttctatggtc tgcgtataag 120
catcgcaaaa cgattgaaaa aagttttaat aaaggctttt atcattaa 168
<210> 258
<211> 55
<212> PRT
<213> 植物乳杆菌
<400> 258
Met Asn Asn Ala Leu Ser Phe Glu Gln Gln Phe Thr Asp Phe Ser Thr
1 5 10 15
Leu Ser Asp Ser Glu Leu Glu Ser Val Glu Gly Gly Arg Asn Lys Leu
20 25 30
Ala Tyr Asn Met Gly His Tyr Ala Gly Lys Ala Thr Ile Phe Gly Leu
35 40 45
Ala Ala Trp Ala Leu Leu Ala
50 55
<210> 259
<211> 168
<212> DNA
<213> 植物乳杆菌
<400> 259
atgaataacg cattaagttt tgaacaacaa tttacagact tcagcacctt atcggactct 60
gaattagaat ccgttgaggg tggccgaaat aagcttgcat ataatatggg gcattacgct 120
ggtaaggcaa ccatttttgg acttgcagca tgggcactcc ttgcatga 168
<210> 260
<211> 47
<212> PRT
<213> 植物乳杆菌
<400> 260
Met Asp Lys Ile Ile Lys Phe Gln Gly Ile Ser Asp Asp Gln Leu Asn
1 5 10 15
Ala Val Ile Gly Gly Lys Lys Lys Lys Gln Ser Trp Tyr Ala Ala Ala
20 25 30
Gly Asp Ala Ile Val Ser Phe Gly Glu Gly Phe Leu Asn Ala Trp
35 40 45
<210> 261
<211> 144
<212> DNA
<213> 植物乳杆菌
<400> 261
atggataaga ttattaagtt tcaagggatt tctgatgatc aattaaatgc tgttatcggt 60
gggaaaaaga aaaaacaatc ttggtacgca gcagctggtg atgcaatcgt tagttttggt 120
gaaggatttt taaatgcttg gtaa 144
<210> 262
<211> 59
<212> PRT
<213> 植物乳杆菌
<400> 262
Met Lys Ile Ser Lys Ile Glu Ala Gln Ala Arg Lys Asp Phe Phe Lys
1 5 10 15
Lys Ile Asp Thr Asn Ser Asn Leu Leu Asn Val Asn Gly Ala Lys Cys
20 25 30
Lys Trp Trp Asn Ile Ser Cys Asp Leu Gly Asn Asn Gly His Val Cys
35 40 45
Thr Leu Ser His Glu Cys Gln Val Ser Cys Asn
50 55
<210> 263
<211> 180
<212> DNA
<213> 植物乳杆菌
<400> 263
atgaaaattt ctaagattga agctcaggct cgtaaagatt tttttaaaaa aatcgatact 60
aactcgaact tattaaatgt aaatggtgcc aaatgcaagt ggtggaatat ttcgtgtgat 120
ttaggaaata atggccatgt ttgtaccttg tcacatgaat gccaagtatc ttgtaactaa 180
<210> 264
<211> 67
<212> PRT
<213> 植物乳杆菌
<400> 264
Met Thr Lys Thr Ser Arg Arg Lys Asn Ala Ile Ala Asn Tyr Leu Glu
1 5 10 15
Pro Val Asp Glu Lys Ser Ile Asn Glu Ser Phe Gly Ala Gly Asp Pro
20 25 30
Glu Ala Arg Ser Gly Ile Pro Cys Thr Ile Gly Ala Ala Val Ala Ala
35 40 45
Ser Ile Ala Val Cys Pro Thr Thr Lys Cys Ser Lys Arg Cys Gly Lys
50 55 60
Arg Lys Lys
65
<210> 265
<211> 204
<212> DNA
<213> 植物乳杆菌
<400> 265
atgactaaaa ctagtcgtcg taagaatgct attgctaatt atttagaacc agtcgacgaa 60
aaaagtatta atgaatcttt tggggctggg gatccggaag caagatccgg aattccatgt 120
acaatcggcg cagctgtcgc agcatcaatt gcagtttgtc caactactaa gtgtagtaaa 180
cgttgtggca agcgtaagaa ataa 204
<210> 266
<211> 48
<212> PRT
<213> 植物乳杆菌
<400> 266
Met Lys Ile Gln Ile Lys Gly Met Lys Gln Leu Ser Asn Lys Glu Met
1 5 10 15
Gln Lys Ile Val Gly Gly Lys Ser Ser Ala Tyr Ser Leu Gln Met Gly
20 25 30
Ala Thr Ala Ile Lys Gln Val Lys Lys Leu Phe Lys Lys Trp Gly Trp
35 40 45
<210> 267
<211> 147
<212> DNA
<213> 植物乳杆菌
<400> 267
atgaaaattc aaattaaagg tatgaagcaa cttagtaata aggaaatgca aaaaatagta 60
ggtggaaaga gtagtgcgta ttctttgcag atgggggcaa ctgcaattaa acaggtaaag 120
aaactgttta aaaaatgggg atggtaa 147
<210> 268
<211> 207
<212> PRT
<213> 詹氏丙酸杆菌
<400> 268
Met Asn Lys Thr His Lys Met Ala Thr Leu Val Ile Ala Ala Ile Leu
1 5 10 15
Ala Ala Gly Met Thr Ala Pro Thr Ala Tyr Ala Asp Ser Pro Gly Asn
20 25 30
Thr Arg Ile Thr Ala Ser Glu Gln Ser Val Leu Thr Gln Ile Leu Gly
35 40 45
His Lys Pro Thr Gln Thr Glu Tyr Asn Arg Tyr Val Glu Thr Tyr Gly
50 55 60
Ser Val Pro Thr Glu Ala Asp Ile Asn Ala Tyr Ile Glu Ala Ser Glu
65 70 75 80
Ser Glu Gly Ser Ser Ser Gln Thr Ala Ala His Asp Asp Ser Thr Ser
85 90 95
Pro Gly Thr Ser Thr Glu Ile Tyr Thr Gln Ala Ala Pro Ala Arg Phe
100 105 110
Ser Met Phe Phe Leu Ser Gly Thr Trp Ile Thr Arg Ser Gly Val Val
115 120 125
Ser Leu Ser Leu Lys Pro Arg Lys Gly Gly Ile Gly Asn Glu Gly Asp
130 135 140
Glu Arg Thr Trp Lys Thr Val Tyr Asp Lys Phe His Asn Ala Gly Gln
145 150 155 160
Trp Thr Arg Tyr Lys Asn Asn Gly Val Asp Ala Ser Met Lys Lys Gln
165 170 175
Tyr Met Cys His Phe Lys Tyr Gly Met Val Lys Thr Pro Trp Asn Leu
180 185 190
Glu Pro His Lys Lys Ala Ala Asp Val Ser Pro Val Lys Cys Asn
195 200 205
<210> 269
<211> 624
<212> DNA
<213> 詹氏丙酸杆菌
<400> 269
atgaacaaaa cacacaaaat ggcgacgctg gtaattgccg cgatcttggc cgccggaatg 60
accgcaccaa ctgcctatgc agattctcct ggaaacacca gaattacagc cagcgagcaa 120
agcgtcctta cccagatact cggccacaaa cctacacaaa ctgaatataa ccgatacgtt 180
gagacttacg gaagcgtacc gaccgaagca gacatcaacg catatataga agcgtctgaa 240
tctgagggat catcaagtca aacggctgct cacgatgact cgacatcacc cggcacgagt 300
accgaaatct acacgcaggc agcccctgcc aggttctcaa tgtttttcct gtccggaact 360
tggatcacta ggagtggtgt agtatcgctc tccttgaagc caaggaaggg tggtattggc 420
aacgaggggg acgagcgtac ctggaagact gtatacgaca aattccataa cgctgggcaa 480
tggacacgat acaagaacaa cggcgtagac gccagcatga aaaagcagta catgtgccac 540
ttcaagtacg ggatggtgaa gacgccatgg aatctggagc cccacaagaa ggctgcagac 600
gtcagtccag tcaagtgcaa ctag 624
<210> 270
<211> 96
<212> PRT
<213> 苏氏丙酸杆菌
<400> 270
Met Lys Lys Thr Leu Leu Arg Ser Gly Thr Ile Ala Leu Ala Thr Ala
1 5 10 15
Ala Ala Phe Gly Ala Ser Leu Ala Ala Ala Pro Ser Ala Met Ala Val
20 25 30
Pro Gly Gly Cys Thr Tyr Thr Arg Ser Asn Arg Asp Val Ile Gly Thr
35 40 45
Cys Lys Thr Gly Ser Gly Gln Phe Arg Ile Arg Leu Asp Cys Asn Asn
50 55 60
Ala Pro Asp Lys Thr Ser Val Trp Ala Lys Pro Lys Val Met Val Ser
65 70 75 80
Val His Cys Leu Val Gly Gln Pro Arg Ser Ile Ser Phe Glu Thr Lys
85 90 95
<210> 271
<211> 291
<212> DNA
<213> 苏氏丙酸杆菌
<400> 271
atgaagaaga ccctcctgcg aagtggaacg atcgcactgg cgaccgcggc tgcatttggc 60
gcatcattgg cagccgcccc atctgccatg gccgttcctg gtggttgcac gtacacaaga 120
agcaatcgcg acgtcatcgg tacctgcaag actggaagcg gccagttccg aatccgactt 180
gactgcaaca acgctccaga caaaacttca gtctgggcca agcccaaggt aatggtgtcg 240
gttcactgtc ttgttggtca accgaggtcc atctcgttcg agaccaagtg a 291
<210> 272
<211> 255
<212> PRT
<213> 费氏丙酸杆菌佛氏亚种
<400> 272
Met Asn Thr Lys Ala Val Asn Leu Lys Ser Glu Asn Thr Thr Lys Leu
1 5 10 15
Val Ser Tyr Leu Thr Glu Asn Gln Leu Asp Glu Phe Ile Arg Arg Ile
20 25 30
Arg Ile Asp Gly Ala Leu Val Glu Glu Val Ser Gln Asn Ala Lys Gln
35 40 45
Ala Leu Asp Asn Thr Gly Leu Asn Gly Trp Ile Asn Thr Asp Cys Asp
50 55 60
Glu Gly Leu Leu Ser Asp Phe Ile Ser Lys Ile Ala Ser Ala Arg Trp
65 70 75 80
Ile Pro Leu Ala Glu Ser Ile Arg Pro Ala Val Thr Asp Arg Asp Lys
85 90 95
Tyr Arg Val Ser Cys Trp Phe Tyr Gln Gly Met Asn Ile Ala Ile Tyr
100 105 110
Ala Asn Ile Gly Gly Val Ala Asn Ile Ile Gly Tyr Thr Glu Ala Ala
115 120 125
Val Ala Thr Leu Leu Gly Ala Val Val Ala Val Ala Pro Val Val Pro
130 135 140
Gly Thr Pro Thr Pro Pro Lys Asp Lys Ser Ser Gln Tyr Lys Glu Val
145 150 155 160
Pro Leu Ala Val Arg Leu Ser Glu Thr Tyr His Glu Glu Gly Val Arg
165 170 175
Gly Leu Phe Asp Glu Leu Asn Tyr Ser Glu Ser Arg Met Ile Ser Thr
180 185 190
Leu Arg Arg Ala Ser Thr Asp Gly Val Leu Ile Asn Ser Trp Asn Asp
195 200 205
Gly Gln Asp Thr Ile Leu Leu Lys Lys Tyr Asn Phe Gln Asp Leu Gln
210 215 220
Leu Thr Val Arg Ser Arg Ile Val Gly Asn Gln Thr Ile Ile Glu Glu
225 230 235 240
Cys Lys Ile Thr Asp Gly Arg Lys Thr Leu Ser Asp Glu Thr Val
245 250 255
<210> 273
<211> 768
<212> DNA
<213> 费氏丙酸杆菌佛氏亚种
<400> 273
atgaatacca aagctgtaaa tctgaagtca gaaaacacga ctaagttggt gagctacctt 60
acggaaaatc aattggatga gtttattaga aggattcgca ttgatggcgc tcttgtggaa 120
gaggtcagtc aaaatgctaa gcaggcctta gataatactg ggctcaatgg ctggataaat 180
actgattgcg atgaaggcct tctctctgat ttcatttcaa agatagcaag tgctagatgg 240
attccattag ctgagtcaat tcgacctgcg gtgactgaca gggataagta tcgagtaagt 300
tgctggttct accaggggat gaatatagca atttacgcaa atatcggtgg cgtggccaat 360
attatcggct atacggaggc cgcagtcgca acactccttg gtgcagttgt ggcggtagct 420
cctgtggtcc ctggaactcc aacccctcca aaggacaaga gttcgcaata taaggaggtt 480
ccccttgccg ttcgtctttc cgaaacatac cacgaagagg gagtacgagg tctattcgac 540
gagctgaact actccgagag ccgtatgatc tctactctaa ggcgagcatc aaccgatgga 600
gtcctaatta attcttggaa cgatgggcag gatacaattc tgcttaagaa gtacaatttc 660
caagacttgc aactgactgt caggagccgc attgttggga atcaaacaat aattgaagaa 720
tgcaaaatca ctgatggtag aaaaactctt tcagacgaga ctgtgtag 768
<210> 274
<211> 618
<212> PRT
<213> 绿脓假单胞菌
<400> 274
Met Ala Arg Pro Ile Ala Asp Leu Ile His Phe Asn Ser Thr Thr Val
1 5 10 15
Thr Ala Ser Gly Asp Val Tyr Tyr Gly Pro Gly Gly Gly Thr Gly Ile
20 25 30
Gly Pro Ile Ala Arg Pro Ile Glu His Gly Leu Asp Ser Ser Thr Glu
35 40 45
Asn Gly Trp Gln Glu Phe Glu Ser Tyr Ala Asp Val Gly Val Asp Pro
50 55 60
Arg Arg Tyr Val Pro Leu Gln Val Lys Glu Lys Arg Arg Glu Ile Glu
65 70 75 80
Leu Gln Phe Arg Asp Ala Glu Lys Lys Leu Glu Ala Ser Val Gln Ala
85 90 95
Glu Leu Asp Lys Ala Asp Ala Ala Leu Gly Pro Ala Lys Asn Leu Ala
100 105 110
Pro Leu Asp Val Ile Asn Arg Ser Leu Thr Ile Val Gly Asn Ala Leu
115 120 125
Gln Gln Lys Asn Gln Lys Leu Leu Leu Asn Gln Lys Lys Ile Thr Ser
130 135 140
Leu Gly Ala Lys Asn Phe Leu Thr Arg Thr Ala Glu Glu Ile Gly Glu
145 150 155 160
Gln Ala Val Arg Glu Gly Asn Ile Asn Gly Pro Glu Ala Tyr Met Arg
165 170 175
Phe Leu Asp Arg Glu Met Glu Gly Leu Thr Ala Ala Tyr Asn Val Lys
180 185 190
Leu Phe Thr Glu Ala Ile Ser Ser Leu Gln Ile Arg Met Asn Thr Leu
195 200 205
Thr Ala Ala Lys Ala Ser Ile Glu Ala Ala Ala Ala Asn Lys Ala Arg
210 215 220
Glu Gln Ala Ala Ala Glu Ala Lys Arg Lys Ala Glu Glu Gln Ala Arg
225 230 235 240
Gln Gln Ala Ala Ile Arg Ala Ala Asn Thr Tyr Ala Met Pro Ala Asn
245 250 255
Gly Ser Val Val Ala Thr Ala Ala Gly Arg Gly Leu Ile Gln Val Ala
260 265 270
Gln Gly Ala Ala Ser Leu Ala Gln Ala Ile Ser Asp Ala Ile Ala Val
275 280 285
Leu Gly Arg Val Leu Ala Ser Ala Pro Ser Val Met Ala Val Gly Phe
290 295 300
Ala Ser Leu Thr Tyr Ser Ser Arg Thr Ala Glu Gln Trp Gln Asp Gln
305 310 315 320
Thr Pro Asp Ser Val Arg Tyr Ala Leu Gly Met Asp Ala Ala Lys Leu
325 330 335
Gly Leu Pro Pro Ser Val Asn Leu Asn Ala Val Ala Lys Ala Ser Gly
340 345 350
Thr Val Asp Leu Pro Met Arg Leu Thr Asn Glu Ala Arg Gly Asn Thr
355 360 365
Thr Thr Leu Ser Val Val Ser Thr Asp Gly Val Ser Val Pro Lys Ala
370 375 380
Val Pro Val Arg Met Ala Ala Tyr Asn Ala Thr Thr Gly Leu Tyr Glu
385 390 395 400
Val Thr Val Pro Ser Thr Thr Ala Glu Ala Pro Pro Leu Ile Leu Thr
405 410 415
Trp Thr Pro Ala Ser Pro Pro Gly Asn Gln Asn Pro Ser Ser Thr Thr
420 425 430
Pro Val Val Pro Lys Pro Val Pro Val Tyr Glu Gly Ala Thr Leu Thr
435 440 445
Pro Val Lys Ala Thr Pro Glu Thr Tyr Pro Gly Val Ile Thr Leu Pro
450 455 460
Glu Asp Leu Ile Ile Gly Phe Pro Ala Asp Ser Gly Ile Lys Pro Ile
465 470 475 480
Tyr Val Met Phe Arg Asp Pro Arg Asp Val Pro Gly Ala Ala Thr Gly
485 490 495
Lys Gly Gln Pro Val Ser Gly Asn Trp Leu Gly Ala Ala Ser Gln Gly
500 505 510
Glu Gly Ala Pro Ile Pro Ser Gln Ile Ala Asp Lys Leu Arg Gly Lys
515 520 525
Thr Phe Lys Asn Trp Arg Asp Phe Arg Glu Gln Phe Trp Ile Ala Val
530 535 540
Ala Asn Asp Pro Glu Leu Ser Lys Gln Phe Asn Pro Gly Ser Leu Ala
545 550 555 560
Val Met Arg Asp Gly Gly Ala Pro Tyr Val Arg Glu Ser Glu Gln Ala
565 570 575
Gly Gly Arg Ile Lys Ile Glu Ile His His Lys Val Arg Val Ala Asp
580 585 590
Gly Gly Gly Val Tyr Asn Met Gly Asn Leu Val Ala Val Thr Pro Lys
595 600 605
Arg His Ile Glu Ile His Lys Gly Gly Lys
610 615
<210> 275
<211> 1857
<212> DNA
<213> 绿脓假单胞菌
<400> 275
atggcacgac ccattgctga ccttatccac ttcaactcta caactgtcac ggcaagcgga 60
gacgtttatt acggccctgg gggaggtacc ggcattggcc ccattgccag acctatagag 120
cacggcttgg attcgtccac tgaaaatggc tggcaagagt ttgaaagtta tgctgatgtg 180
ggcgttgacc ccagacgcta tgttcctctt caggttaaag aaaaacgcag ggagatcgag 240
cttcagttcc gagatgccga gaaaaaactt gaggcgtcgg tacaagccga gctggataag 300
gctgatgccg ctcttggtcc ggcaaagaat cttgcaccat tggacgtcat caaccgcagt 360
ctgaccatcg ttggaaacgc cctccagcaa aagaatcaaa aactactgct gaatcagaag 420
aagattacca gcctgggtgc aaagaatttc cttacccgta cggcggaaga gatcggtgaa 480
caagcggtgc gagaaggcaa tattaacggg cctgaagcct atatgcgctt cctcgacagg 540
gaaatggaag gtctcacggc agcttataac gtaaaactct tcaccgaagc gatcagtagt 600
ctccagatcc gcatgaatac gttgaccgcc gccaaagcaa gtattgaggc ggccgcagca 660
aacaaggcgc gtgaacaagc agcggctgag gccaaacgca aagccgaaga gcaggcccgc 720
cagcaagcgg cgataagagc tgccaatacc tatgccatgc cggccaatgg cagcgttgtc 780
gccaccgccg caggccgggg tctgatccag gtcgcacaag gcgccgcatc ccttgctcaa 840
gcgatctccg atgcgattgc cgtcctgggc cgggtcctgg cttcagcacc ctcggtgatg 900
gccgtgggct ttgccagtct gacctactcc tcccggactg ccgagcaatg gcaggaccaa 960
acgcccgata gcgttcgtta cgccctgggc atggatgccg ctaaattggg gcttccccca 1020
agcgtaaacc tgaacgcggt tgcaaaagcc agcggtaccg tcgatctgcc gatgcgcctg 1080
accaacgagg cacgaggcaa cacgacgacc ctttcggtgg tcagcaccga tggtgtgagc 1140
gttccgaaag ccgttccggt ccggatggcg gcctacaatg ccacgacagg cctgtacgag 1200
gttacggttc cctctacgac cgcagaagcg ccgccactga tcctgacctg gacgccggcg 1260
agtcctccag gaaaccagaa cccttcgagt accactccgg tcgtaccgaa gccggtgccg 1320
gtatatgagg gagcgaccct tacaccggtg aaggctaccc cggaaaccta tcctggggtg 1380
attacactac cggaagacct gatcatcggc ttcccggccg actcggggat caagccgatc 1440
tatgtgatgt tcagggatcc gcgggatgta cctggtgctg cgactggcaa gggacagccc 1500
gtcagcggta attggctcgg cgccgcctct caaggtgagg gggctccaat tccaagccag 1560
attgcggata aactacgtgg taagacattc aaaaactggc gggactttcg ggaacaattc 1620
tggatagctg tggctaatga tcctgagtta agtaaacagt ttaatcctgg tagtttagct 1680
gtaatgagag atggaggggc tccttatgtc agagagtcag aacaggctgg cgggagaata 1740
aagatcgaaa tccaccacaa ggttcgagta gcagatggag gcggcgttta caatatgggg 1800
aaccttgttg cagtaacgcc aaaacgtcat atagaaatcc acaagggagg gaagtga 1857
<210> 276
<211> 689
<212> PRT
<213> 绿脓假单胞菌
<400> 276
Met Ala Val Asn Asp Tyr Glu Pro Gly Ser Met Val Ile Thr His Val
1 5 10 15
Gln Gly Gly Gly Arg Asp Ile Ile Gln Tyr Ile Pro Ala Arg Ser Ser
20 25 30
Tyr Gly Thr Pro Pro Phe Val Pro Pro Gly Pro Ser Pro Tyr Val Gly
35 40 45
Thr Gly Met Gln Glu Tyr Arg Lys Leu Arg Ser Thr Leu Asp Lys Ser
50 55 60
His Ser Glu Leu Lys Lys Asn Leu Lys Asn Glu Thr Leu Lys Glu Val
65 70 75 80
Asp Glu Leu Lys Ser Glu Ala Gly Leu Pro Gly Lys Ala Val Ser Ala
85 90 95
Asn Asp Ile Arg Asp Glu Lys Ser Ile Val Asp Ala Leu Met Asp Ala
100 105 110
Lys Ala Lys Ser Leu Lys Ala Ile Glu Asp Arg Pro Ala Asn Leu Tyr
115 120 125
Thr Ala Ser Asp Phe Pro Gln Lys Ser Glu Ser Met Tyr Gln Ser Gln
130 135 140
Leu Leu Ala Ser Arg Lys Phe Tyr Gly Glu Phe Leu Asp Arg His Met
145 150 155 160
Ser Glu Leu Ala Lys Ala Tyr Ser Ala Asp Ile Tyr Lys Ala Gln Ile
165 170 175
Ala Ile Leu Lys Gln Thr Ser Gln Glu Leu Glu Asn Lys Ala Arg Ser
180 185 190
Leu Glu Ala Glu Ala Gln Arg Ala Ala Ala Glu Val Glu Ala Asp Tyr
195 200 205
Lys Ala Arg Lys Ala Asn Val Glu Lys Lys Val Gln Ser Glu Leu Asp
210 215 220
Gln Ala Gly Asn Ala Leu Pro Gln Leu Thr Asn Pro Thr Pro Glu Gln
225 230 235 240
Trp Leu Glu Arg Ala Thr Gln Leu Val Thr Gln Ala Ile Ala Asn Lys
245 250 255
Lys Lys Leu Gln Thr Ala Asn Asn Ala Leu Ile Ala Lys Ala Pro Asn
260 265 270
Ala Leu Glu Lys Gln Lys Ala Thr Tyr Asn Ala Asp Leu Leu Val Asp
275 280 285
Glu Ile Ala Ser Leu Gln Ala Arg Leu Asp Lys Leu Asn Ala Glu Thr
290 295 300
Ala Arg Arg Lys Glu Ile Ala Arg Gln Ala Ala Ile Arg Ala Ala Asn
305 310 315 320
Thr Tyr Ala Met Pro Ala Asn Gly Ser Val Val Ala Thr Ala Ala Gly
325 330 335
Arg Gly Leu Ile Gln Val Ala Gln Gly Ala Ala Ser Leu Ala Gln Ala
340 345 350
Ile Ser Asp Ala Ile Ala Val Leu Gly Arg Val Leu Ala Ser Ala Pro
355 360 365
Ser Val Met Ala Val Gly Phe Ala Ser Leu Thr Tyr Ser Ser Arg Thr
370 375 380
Ala Glu Gln Trp Gln Asp Gln Thr Pro Asp Ser Val Arg Tyr Ala Leu
385 390 395 400
Gly Met Asp Ala Ala Lys Leu Gly Leu Pro Pro Ser Val Asn Leu Asn
405 410 415
Ala Val Ala Lys Ala Ser Gly Thr Val Asp Leu Pro Met Arg Leu Thr
420 425 430
Asn Glu Ala Arg Gly Asn Thr Thr Thr Leu Ser Val Val Ser Thr Asp
435 440 445
Gly Val Ser Val Pro Lys Ala Val Pro Val Arg Met Ala Ala Tyr Asn
450 455 460
Ala Thr Thr Gly Leu Tyr Glu Val Thr Val Pro Ser Thr Thr Ala Glu
465 470 475 480
Ala Pro Pro Leu Ile Leu Thr Trp Thr Pro Ala Ser Pro Pro Gly Asn
485 490 495
Gln Asn Pro Ser Ser Thr Thr Pro Val Val Pro Lys Pro Val Pro Val
500 505 510
Tyr Glu Gly Ala Thr Leu Thr Pro Val Lys Ala Thr Pro Glu Thr Tyr
515 520 525
Pro Gly Val Ile Thr Leu Pro Glu Asp Leu Ile Ile Gly Phe Pro Ala
530 535 540
Asp Ser Gly Ile Lys Pro Ile Tyr Val Met Phe Arg Asp Pro Arg Asp
545 550 555 560
Val Pro Gly Ala Ala Thr Gly Lys Gly Gln Pro Val Ser Gly Asn Trp
565 570 575
Leu Gly Ala Ala Ser Gln Gly Glu Gly Ala Pro Ile Pro Ser Gln Ile
580 585 590
Ala Asp Lys Leu Arg Gly Lys Thr Phe Lys Asn Trp Arg Asp Phe Arg
595 600 605
Glu Gln Phe Trp Ile Ala Val Ala Asn Asp Pro Glu Leu Ser Lys Gln
610 615 620
Phe Asn Pro Gly Ser Leu Ala Val Met Arg Asp Gly Gly Ala Pro Tyr
625 630 635 640
Val Arg Glu Ser Glu Gln Ala Gly Gly Arg Ile Lys Ile Glu Ile His
645 650 655
His Lys Val Arg Ile Ala Asp Gly Gly Gly Val Tyr Asn Met Gly Asn
660 665 670
Leu Val Ala Val Thr Pro Lys Arg His Ile Glu Ile His Lys Gly Gly
675 680 685
Lys
<210> 277
<211> 2070
<212> DNA
<213> 绿脓假单胞菌
<400> 277
atggctgtca atgattacga acctggttcg atggttatta cacatgtgca gggtggtggg 60
cgtgacataa tccagtatat tcctgctcga tcaagctacg gtactccacc atttgtccca 120
ccaggaccaa gtccgtatgt cggtactgga atgcaggagt acaggaagct aagaagtacg 180
cttgataagt cccattcaga actcaagaaa aacctgaaaa atgaaaccct gaaggaggtt 240
gatgaactca agagtgaagc ggggttgcca ggtaaagcgg tcagtgccaa tgacatccgc 300
gatgaaaaga gtatcgttga tgcactcatg gatgccaaag caaaatcgct aaaggccatt 360
gaggatcgcc cggccaatct ttatacggct tcagactttc ctcagaagtc agagtcgatg 420
taccagagtc agttgctggc cagccgaaaa ttctatggag agttcctgga tcgccatatg 480
agtgagctgg ccaaagcgta cagcgccgat atctataagg cgcaaatcgc tatcttgaaa 540
caaacgtctc aagagctgga gaataaagcc cggtcattgg aagcagaagc ccagcgagcc 600
gctgctgagg tggaggcgga ctacaaggcc aggaaggcaa atgtcgagaa aaaagtgcag 660
tccgagcttg accaggctgg gaatgctttg cctcaactga ccaatccaac gccagagcag 720
tggcttgaac gcgctactca actggttacg caggcgatcg ccaataagaa gaaattgcag 780
actgcaaaca atgccttgat tgccaaggca cccaatgcac tggagaaaca aaaggcaacc 840
tacaacgccg atctcctagt ggatgaaatc gccagcctgc aagcacggct ggacaagctg 900
aacgccgaaa cggcaaggcg caaggaaatc gctcgtcaag cggcgatcag ggctgccaat 960
acttatgcca tgccagccaa tggcagcgtt gtcgccaccg ccgcaggccg gggtctgatc 1020
caggtcgcac aaggcgccgc atcccttgct caagcgatct ccgatgcgat tgccgtcctg 1080
ggccgggtcc tggcttcagc accctcggtg atggccgtgg gctttgccag tctgacctac 1140
tcctcccgga ctgccgagca atggcaggac caaacgcccg atagcgttcg ttacgccctg 1200
ggcatggatg ccgctaaatt ggggcttccc ccaagcgtaa acctgaacgc ggttgcaaaa 1260
gccagcggta ccgtcgatct gccgatgcgc ctgaccaacg aggcacgagg caacacgacg 1320
accctttcgg tggtcagcac cgatggtgtg agcgttccga aagccgttcc ggtccggatg 1380
gcggcctaca atgccacgac aggcctgtac gaggttacgg ttccctctac gaccgcagaa 1440
gcgccgccac tgatcctgac ctggacgccg gcgagtcctc caggaaacca gaacccttcg 1500
agtaccactc cggtcgtacc gaagccggtg ccggtatatg agggagcgac ccttacaccg 1560
gtgaaggcta ccccggaaac ctatcctggg gtgattacac taccggaaga cctgatcatc 1620
ggcttcccgg ccgactcggg gatcaagccg atctatgtga tgttcaggga tccgcgggat 1680
gtacctggtg ctgcgactgg caagggacag cccgtcagcg gtaattggct cggcgccgcc 1740
tctcaaggtg agggggctcc aattccaagc cagattgcgg ataaactacg tggtaagaca 1800
ttcaaaaact ggcgggactt tcgggaacaa ttctggatag ctgtggctaa tgatcctgag 1860
ttaagtaaac agtttaatcc tggtagttta gctgtaatga gagatggagg ggctccttat 1920
gtcagagagt cagaacaggc tggcgggaga ataaagatcg aaatccacca caaggttcga 1980
atagcagatg gaggcggcgt ttacaatatg gggaaccttg ttgcagtaac gccaaaacgt 2040
catatagaaa tccacaaggg agggaagtga 2070
<210> 278
<211> 47
<212> PRT
<213> 活泼瘤胃球菌
<400> 278
Met Arg Asn Asp Val Leu Thr Leu Thr Asn Pro Met Glu Glu Lys Glu
1 5 10 15
Leu Glu Gln Ile Leu Gly Gly Gly Asn Gly Val Leu Lys Thr Ile Ser
20 25 30
His Glu Cys Asn Met Asn Thr Trp Gln Phe Leu Phe Thr Cys Cys
35 40 45
<210> 279
<211> 144
<212> DNA
<213> 活泼瘤胃球菌
<400> 279
atgagaaatg acgtattaac attaacaaac ccaatggaag agaacgaact ggagcagatc 60
ttaggtggtg gcaatggtgt gttaaaaacg attagccacg aatgcaatat gaacacatgg 120
cagttcctgt ttacttgttg ctaa 144
<210> 280
<211> 55
<212> PRT
<213> 清酒乳杆菌
<400> 280
Met Lys Asn Ala Lys Ser Leu Thr Ile Gln Glu Met Lys Ser Ile Thr
1 5 10 15
Gly Gly Lys Tyr Tyr Gly Asn Gly Val Ser Cys Asn Ser His Gly Cys
20 25 30
Ser Val Asn Trp Gly Gln Ala Trp Thr Cys Gly Val Asn His Leu Ala
35 40 45
Asn Gly Gly His Gly Val Cys
50 55
<210> 281
<211> 168
<212> DNA
<213> 清酒乳杆菌
<400> 281
atgaaaaacg caaaaagcct aacaattcaa gaaatgaaat ctattacagg tggtaaatac 60
tatggtaatg gcgttagctg taactctcac ggctgttcag taaattgggg gcaagcatgg 120
acttgtggag taaaccatct agctaatggc ggtcatggag tttgttaa 168
<210> 282
<211> 59
<212> PRT
<213> 清酒乳杆菌
<400> 282
Met Asn Asn Val Lys Glu Leu Ser Met Thr Glu Leu Gln Thr Ile Thr
1 5 10 15
Gly Gly Ala Arg Ser Tyr Gly Asn Gly Val Tyr Cys Asn Asn Lys Lys
20 25 30
Cys Trp Val Asn Arg Gly Glu Ala Thr Gln Ser Ile Ile Gly Gly Met
35 40 45
Ile Ser Gly Trp Ala Ser Gly Leu Ala Gly Met
50 55
<210> 283
<211> 180
<212> DNA
<213> 清酒乳杆菌
<400> 283
atgaataatg taaaagaatt aagtatgaca gaattacaaa caattaccgg cggtgctaga 60
tcatatggca acggtgttta ctgtaataat aaaaaatgtt gggtaaatcg gggtgaagca 120
acgcaaagta ttattggtgg tatgattagc ggctgggcta gtggtttagc tggaatgtaa 180
<210> 284
<211> 61
<212> PRT
<213> 清酒乳杆菌
<400> 284
Met Glu Lys Phe Ile Glu Leu Ser Leu Lys Glu Val Thr Ala Ile Thr
1 5 10 15
Gly Gly Lys Tyr Tyr Gly Asn Gly Val His Cys Gly Lys His Ser Cys
20 25 30
Thr Val Asp Trp Gly Thr Ala Ile Gly Asn Ile Gly Asn Asn Ala Ala
35 40 45
Ala Asn Trp Ala Thr Gly Gly Asn Ala Gly Trp Asn Lys
50 55 60
<210> 285
<211> 186
<212> DNA
<213> 清酒乳杆菌
<400> 285
atggaaaagt ttattgaatt atctttaaaa gaagtaacag caattacagg tggaaaatat 60
tatggtaacg gtgtacactg tggaaaacat tcatgtaccg tagactgggg aacagctatt 120
ggaaatatcg gaaataatgc agctgcaaac tgggccacag gcggaaacgc tggctggaat 180
aaataa 186
<210> 286
<211> 56
<212> PRT
<213> 唾液链球菌
<400> 286
Met Lys Ser Thr Asn Asn Gln Ser Ile Ala Glu Ile Ala Ala Val Asn
1 5 10 15
Ser Leu Gln Glu Val Ser Met Glu Glu Leu Asp Gln Ile Ile Gly Ala
20 25 30
Gly Asn Gly Val Val Leu Thr Leu Thr His Glu Cys Asn Leu Ala Thr
35 40 45
Trp Thr Lys Lys Leu Lys Cys Cys
50 55
<210> 287
<211> 171
<212> DNA
<213> 唾液链球菌
<400> 287
atgaaatcaa caaataatca aagtatcgca gaaattgcag cagtaaactc actacaagaa 60
gtaagtatgg aggaactaga ccaaattatt ggtgccggaa acggagtggt tcttactctt 120
actcatgaat gtaacctagc aacttggaca aaaaaactaa aatgttgcta a 171
<210> 288
<211> 51
<212> PRT
<213> 化脓性链球菌血清型M28
<400> 288
Met Ser Phe Met Lys Asn Ser Lys Asp Ile Leu Thr Asn Ala Ile Glu
1 5 10 15
Glu Val Ser Glu Lys Glu Leu Met Glu Val Ala Gly Gly Lys Lys Gly
20 25 30
Ser Gly Trp Phe Ala Thr Ile Thr Asp Asp Cys Pro Asn Ser Val Phe
35 40 45
Val Cys Cys
50
<210> 289
<211> 156
<212> DNA
<213> 化脓性链球菌血清型M28
<400> 289
atgagtttta tgaaaaattc aaaggatatt ttgactaatg ctatcgaaga agtttctgaa 60
aaagaactta tggaagtagc tggtggtaaa aaaggttccg gttggtttgc aactattact 120
gatgactgtc cgaactcagt attcgtttgt tgttaa 156
<210> 290
<211> 48
<212> PRT
<213> 唾液链球菌
<400> 290
Met Lys Asn Ser Lys Asp Val Leu Asn Asn Ala Ile Glu Glu Val Ser
1 5 10 15
Glu Lys Glu Leu Met Glu Val Ala Gly Gly Lys Lys Gly Pro Gly Trp
20 25 30
Ile Ala Thr Ile Thr Asp Asp Cys Pro Asn Ser Ile Phe Val Cys Cys
35 40 45
<210> 291
<211> 147
<212> DNA
<213> 唾液链球菌
<400> 291
atgaaaaact caaaagatgt tttgaacaat gctatcgaag aggtttctga aaaagaactt 60
atggaagtag ctggtggtaa aaaaggtcca ggttggattg caactattac tgatgactgt 120
ccaaactcaa tattcgtttg ttgttaa 147
<210> 292
<211> 48
<212> PRT
<213> 唾液链球菌
<400> 292
Met Lys Asn Ser Lys Asp Ile Leu Asn Asn Ala Ile Glu Glu Val Ser
1 5 10 15
Glu Lys Glu Leu Met Glu Val Ala Gly Gly Lys Arg Gly Ser Gly Trp
20 25 30
Ile Ala Thr Ile Thr Asp Asp Cys Pro Asn Ser Val Phe Val Cys Cys
35 40 45
<210> 293
<211> 147
<212> DNA
<213> 唾液链球菌
<400> 293
atgaaaaact caaaagatat tttgaacaat gctatcgaag aagtttctga aaaagaactt 60
atggaagtag ctggtggtaa aagaggttca ggttggattg caactattac tgatgactgt 120
ccaaactcag tattcgtttg ttgttaa 147
<210> 294
<211> 62
<212> PRT
<213> 金黄色葡萄球菌
<400> 294
Met Lys Ser Ser Phe Leu Glu Lys Asp Ile Glu Glu Gln Val Thr Trp
1 5 10 15
Phe Glu Glu Val Ser Glu Gln Glu Phe Asp Asp Asp Ile Phe Gly Ala
20 25 30
Cys Ser Thr Asn Thr Phe Ser Leu Ser Asp Tyr Trp Gly Asn Lys Gly
35 40 45
Asn Trp Cys Thr Ala Thr His Glu Cys Met Ser Trp Cys Lys
50 55 60
<210> 295
<211> 189
<212> DNA
<213> 金黄色葡萄球菌
<400> 295
atgaaaagtt cttttttaga aaaagatata gaagaacaag tgacatggtt cgaggaagtt 60
tcagaacaag aatttgacga tgatattttt ggagcttgta gtacaaacac tttttctttg 120
agtgactatt ggggtaataa aggaaattgg tgtactgcta ctcacgaatg tatgtcttgg 180
tgtaaataa 189
<210> 296
<211> 67
<212> PRT
<213> 金黄色葡萄球菌
<400> 296
Met Lys Asn Glu Leu Gly Lys Phe Leu Glu Glu Asn Glu Leu Glu Leu
1 5 10 15
Gly Lys Phe Ser Glu Ser Asp Met Leu Glu Ile Thr Asp Asp Glu Val
20 25 30
Tyr Ala Ala Gly Thr Pro Leu Ala Leu Leu Gly Gly Ala Ala Thr Gly
35 40 45
Val Ile Gly Tyr Ile Ser Asn Gln Thr Cys Pro Thr Thr Ala Cys Thr
50 55 60
Arg Ala Cys
65
<210> 297
<211> 204
<212> DNA
<213> 金黄色葡萄球菌
<400> 297
atgaaaaatg aattaggtaa gtttttagaa gaaaacgaat tagagttagg taaattttca 60
gaatcagaca tgctagaaat tactgatgat gaagtatatg cagctggaac acctttagcc 120
ttattgggtg gagctgccac cggggtgata ggttatattt ctaaccaaac atgtccaaca 180
actgcttgta cacgcgcttg ctag 204
<210> 298
<211> 46
<212> PRT
<213> 化脓性链球菌
<400> 298
Met Asn Asn Thr Ile Lys Asp Phe Asp Leu Asp Leu Lys Thr Asn Lys
1 5 10 15
Lys Asp Thr Ala Thr Pro Tyr Val Gly Ser Arg Tyr Leu Cys Thr Pro
20 25 30
Gly Ser Cys Trp Lys Leu Val Cys Phe Thr Thr Thr Val Lys
35 40 45
<210> 299
<211> 141
<212> DNA
<213> 化脓性链球菌
<400> 299
atgaataaca caattaaaga ctttgatctc gatttgaaaa caaataaaaa agacactgct 60
acaccttatg ttggtagccg ttacctatgt acccctggtt cttgttggaa attagtttgc 120
tttacaacaa ctgttaaata a 141
<210> 300
<211> 51
<212> PRT
<213> 化脓性链球菌
<400> 300
Met Glu Lys Asn Asn Glu Val Ile Asn Ser Ile Gln Glu Val Ser Leu
1 5 10 15
Glu Glu Leu Asp Gln Ile Ile Gly Ala Gly Lys Asn Gly Val Phe Lys
20 25 30
Thr Ile Ser His Glu Cys His Leu Asn Thr Trp Ala Phe Leu Ala Thr
35 40 45
Cys Cys Ser
50
<210> 301
<211> 156
<212> DNA
<213> 化脓性链球菌
<400> 301
atggaaaaaa ataatgaagt aatcaactct attcaagaag ttagtcttga agaactcgat 60
caaattatcg gtgctggaaa aaatggtgtg tttaaaacaa tttctcatga gtgtcatttg 120
aatacatggg cattccttgc tacttgttgt tcataa 156
<210> 302
<211> 51
<212> PRT
<213> 化脓性链球菌血清型M49
<400> 302
Met Thr Lys Glu His Glu Ile Ile Asn Ser Ile Gln Glu Val Ser Leu
1 5 10 15
Glu Glu Leu Asp Gln Ile Ile Gly Ala Gly Lys Asn Gly Val Phe Lys
20 25 30
Thr Ile Ser His Glu Cys His Leu Asn Thr Trp Ala Phe Leu Ala Thr
35 40 45
Cys Cys Ser
50
<210> 303
<211> 156
<212> DNA
<213> 化脓性链球菌血清型M49
<400> 303
atggaaaaaa ataatgaagt aatcaactct attcaagaag ttagtcttga agaactcgat 60
caaattatcg gtgctggaaa aaatggtgtg tttaaaacaa tttctcatga gtgtcatttg 120
aatacatggg cattccttgc tacttgttgc tcataa 156
<210> 304
<211> 56
<212> PRT
<213> 枯草芽孢杆菌
<400> 304
Met Glu Lys Leu Phe Lys Glu Val Lys Leu Glu Glu Leu Glu Asn Gln
1 5 10 15
Lys Gly Ser Gly Leu Gly Lys Ala Gln Cys Ala Ala Leu Trp Leu Gln
20 25 30
Cys Ala Ser Gly Gly Thr Ile Gly Cys Gly Gly Gly Ala Val Ala Cys
35 40 45
Gln Asn Tyr Arg Gln Phe Cys Arg
50 55
<210> 305
<211> 171
<212> DNA
<213> 枯草芽孢杆菌
<400> 305
atggaaaagc tatttaaaga agttaaacta gaggaactcg aaaaccaaaa aggtagtgga 60
ttaggaaaag ctcagtgtgc tgcgttgtgg ctacaatgtg ctagtggcgg tacaattggt 120
tgtggtggcg gagctgttgc ttgtcaaaac tatcgtcaat tctgcagata a 171
<210> 306
<211> 56
<212> PRT
<213> 枯草芽孢杆菌
<400> 306
Met Ser Lys Phe Asp Asp Phe Asp Leu Asp Val Val Lys Val Ser Lys
1 5 10 15
Gln Asp Ser Lys Ile Thr Pro Gln Trp Lys Ser Glu Ser Leu Cys Thr
20 25 30
Pro Gly Cys Val Thr Gly Ala Leu Gln Thr Cys Phe Leu Gln Thr Leu
35 40 45
Thr Cys Asn Cys Lys Ile Ser Lys
50 55
<210> 307
<211> 171
<212> DNA
<213> 枯草芽孢杆菌
<400> 307
atgtcaaagt tcgatgattt cgatttggat gttgtgaaag tctctaaaca agactcaaaa 60
atcactccgc aatggaaaag tgaatcactt tgtacaccag gatgtgtaac tggtgcattg 120
caaacttgct tccttcaaac actaacttgt aactgcaaaa tctctaaata a 171
<210> 308
<211> 50
<212> PRT
<213> 枯草芽孢杆菌
<400> 308
Met Lys Leu Pro Val Gln Gln Val Tyr Ser Val Tyr Gly Gly Lys Asp
1 5 10 15
Leu Pro Lys Gly His Ser His Ser Thr Met Pro Phe Leu Ser Lys Leu
20 25 30
Gln Phe Leu Thr Lys Ile Tyr Leu Leu Asp Ile His Thr Gln Pro Phe
35 40 45
Phe Ile
50
<210> 309
<211> 153
<212> DNA
<213> 枯草芽孢杆菌
<400> 309
ttgaaattgc cggtgcaaca ggtctattcg gtctatgggg gtaaggatct cccaaaaggg 60
catagtcatt ctactatgcc ctttttaagt aaattacaat ttttaactaa aatctacctc 120
ttggatatac atacacaacc gtttttcatt tga 153
<210> 310
<211> 43
<212> PRT
<213> 枯草芽孢杆菌
<400> 310
Met Lys Lys Ala Val Ile Val Glu Asn Lys Gly Cys Ala Thr Cys Ser
1 5 10 15
Ile Gly Ala Ala Cys Leu Val Asp Gly Pro Ile Pro Asp Phe Glu Ile
20 25 30
Ala Gly Ala Thr Gly Leu Phe Gly Leu Trp Gly
35 40
<210> 311
<211> 132
<212> DNA
<213> 枯草芽孢杆菌
<400> 311
atgaaaaaag ctgtcattgt agaaaacaaa ggttgtgcaa catgctcgat cggagccgct 60
tgtctagtgg acggtcctat ccctgatttt gaaattgccg gtgcaacagg tctattcggt 120
ctatgggggt aa 132
<210> 312
<211> 58
<212> PRT
<213> 嗜热链球菌
<400> 312
Met Met Asn Ala Thr Glu Asn Gln Ile Phe Val Glu Thr Val Ser Asp
1 5 10 15
Gln Glu Leu Glu Met Leu Ile Gly Gly Ala Asp Arg Gly Trp Ile Lys
20 25 30
Thr Leu Thr Lys Asp Cys Pro Asn Val Ile Ser Ser Ile Cys Ala Gly
35 40 45
Thr Ile Ile Thr Ala Cys Lys Asn Cys Ala
50 55
<210> 313
<211> 177
<212> DNA
<213> 嗜热链球菌
<400> 313
atgatgaatg ctactgaaaa ccaaattttt gttgagactg tgagtgacca agaattagaa 60
atgttaattg gtggtgcaga tcgtggatgg attaagactt taacaaaaga ttgtccaaat 120
gtaatttctt caatttgtgc aggtacaatt attacagcct gtaaaaattg tgcttaa 177
<210> 314
<211> 64
<212> PRT
<213> 嗜热链球菌
<400> 314
Met Lys Gln Tyr Asn Gly Phe Glu Val Leu His Glu Leu Asp Leu Ala
1 5 10 15
Asn Val Thr Gly Gly Gln Ile Asn Trp Gly Ser Val Val Gly His Cys
20 25 30
Ile Gly Gly Ala Ile Ile Gly Gly Ala Phe Ser Gly Gly Ala Ala Ala
35 40 45
Gly Val Gly Cys Leu Val Gly Ser Gly Lys Ala Ile Ile Asn Gly Leu
50 55 60
<210> 315
<211> 195
<212> DNA
<213> 嗜热链球菌
<400> 315
atgaagcagt ataatggttt tgaggttcta catgaacttg acttagcaaa tgtaactggc 60
ggtcaaatta attggggatc agttgtagga cactgtatag gtggagctat tatcggaggt 120
gcattttcag gaggtgcagc ggctggagta ggatgccttg ttgggagcgg aaaggcaatc 180
ataaatggat tataa 195
<210> 316
<211> 85
<212> PRT
<213> 嗜热链球菌
<400> 316
Met Asn Thr Ile Thr Ile Cys Lys Phe Asp Val Leu Asp Ala Glu Leu
1 5 10 15
Leu Ser Thr Val Glu Gly Gly Tyr Ser Gly Lys Asp Cys Leu Lys Asp
20 25 30
Met Gly Gly Tyr Ala Leu Ala Gly Ala Gly Ser Gly Ala Leu Trp Gly
35 40 45
Ala Pro Ala Gly Gly Val Gly Ala Leu Pro Gly Ala Phe Val Gly Ala
50 55 60
His Val Gly Ala Ile Ala Gly Gly Phe Ala Cys Met Gly Gly Met Ile
65 70 75 80
Gly Asn Lys Phe Asn
85
<210> 317
<211> 258
<212> DNA
<213> 嗜热链球菌
<400> 317
atgaatacaa taactatttg taaatttgat gttttagatg ctgaacttct ttcgacagtt 60
gagggtggat actctggtaa ggattgttta aaagacatgg gaggatatgc attggcagga 120
gctggaagtg gagctctgtg gggagctcca gcaggaggtg ttggagcact tccaggtgca 180
tttgtcggag ctcatgttgg ggcaattgca ggaggctttg catgtatggg tggaatgatt 240
ggtaataagt ttaactaa 258
<210> 318
<211> 52
<212> PRT
<213> 蜡样芽胞杆菌
<400> 318
Met Ser Glu Ile Lys Lys Ala Leu Asn Thr Leu Glu Ile Glu Asp Phe
1 5 10 15
Asp Ala Ile Glu Met Val Asp Val Asp Ala Met Pro Glu Asn Glu Ala
20 25 30
Leu Glu Ile Met Gly Ala Ser Cys Thr Thr Cys Val Cys Thr Cys Ser
35 40 45
Cys Cys Thr Thr
50
<210> 319
<211> 159
<212> DNA
<213> 蜡样芽胞杆菌
<400> 319
atgagtgaaa ttaaaaaagc attaaatacg cttgaaattg aagattttga tgcaattgaa 60
atggttgatg ttgatgctat gccagaaaac gaagcgcttg aaattatggg agcgtcatgt 120
acgacatgcg tatgtacatg cagttgttgt acaacttga 159
<210> 320
<211> 47
<212> PRT
<213> 蜡样芽胞杆菌
<400> 320
Met Glu Val Met Asn Asn Ala Leu Ile Thr Lys Val Asp Glu Glu Ile
1 5 10 15
Gly Gly Asn Ala Ala Cys Val Ile Gly Cys Ile Gly Ser Cys Val Ile
20 25 30
Ser Glu Gly Ile Gly Ser Leu Val Gly Thr Ala Phe Thr Leu Gly
35 40 45
<210> 321
<211> 144
<212> DNA
<213> 蜡样芽胞杆菌
<400> 321
atggaagtta tgaacaatgc tttaattaca aaagtagatg aggagattgg aggaaacgct 60
gcttgtgtaa ttggttgtat tggcagttgc gtaattagtg aaggaattgg ttcacttgta 120
ggaacagcat ttactttagg ttaa 144
<210> 322
<211> 49
<212> PRT
<213> 蜡样芽胞杆菌
<400> 322
Met Glu Val Leu Asn Lys Gln Asn Val Asn Ile Ile Pro Glu Ser Glu
1 5 10 15
Glu Val Gly Gly Trp Val Ala Cys Val Gly Ala Cys Gly Thr Val Cys
20 25 30
Leu Ala Ser Gly Gly Val Gly Thr Glu Phe Ala Ala Ala Ser Tyr Phe
35 40 45
Leu
<210> 323
<211> 150
<212> DNA
<213> 蜡样芽胞杆菌
<400> 323
atggaagttt taaacaaaca aaatgtaaat attattccag aatctgaaga agtaggtgga 60
tgggtagcat gtgttggagc atgtggtaca gtatgtcttg ctagtggtgg tgttggaaca 120
gagtttgcag ctgcatctta tttcctataa 150
<210> 324
<211> 40
<212> PRT
<213> 苏云金芽孢杆菌
<400> 324
Met Glu Thr Pro Val Val Gln Pro Arg Asp Trp Thr Cys Trp Ser Cys
1 5 10 15
Leu Val Cys Ala Ala Cys Ser Val Glu Leu Leu Asn Leu Val Thr Ala
20 25 30
Ala Thr Gly Ala Ser Thr Ala Ser
35 40
<210> 325
<211> 123
<212> DNA
<213> 苏云金芽孢杆菌
<400> 325
atggaaacac cagtagtaca accaagggat tggacttgtt ggagttgctt agtatgtgca 60
gcatgttctg tggaattatt aaatttagtt actgcggcaa caggggctag tactgcaagc 120
taa 123
<210> 326
<211> 42
<212> PRT
<213> 豆科根瘤菌 三叶草
<400> 326
Met Asp Asn Lys Val Ala Lys Asn Val Glu Val Lys Lys Gly Ser Ile
1 5 10 15
Lys Ala Thr Phe Lys Ala Ala Val Leu Lys Ser Lys Thr Lys Val Asp
20 25 30
Ile Gly Gly Ser Arg Gln Gly Cys Val Ala
35 40
<210> 327
<211> 129
<212> DNA
<213> 豆科根瘤菌 三叶草
<400> 327
atggataaca aggttgcgaa gaatgtcgaa gtgaagaagg gctccatcaa ggcgaccttc 60
aaggctgctg ttctgaagtc gaagacgaag gtcgacatcg gaggtagccg tcagggctgc 120
gtcgcttaa 129
<210> 328
<211> 70
<212> PRT
<213> ***链球菌
<400> 328
Met Asn Thr Ile Glu Lys Phe Glu Asn Ile Lys Leu Phe Ser Leu Lys
1 5 10 15
Lys Ile Ile Gly Gly Lys Thr Val Asn Tyr Gly Asn Gly Leu Tyr Cys
20 25 30
Asn Gln Lys Lys Cys Trp Val Asn Trp Ser Glu Thr Ala Thr Thr Ile
35 40 45
Val Asn Asn Ser Ile Met Asn Gly Leu Thr Gly Gly Asn Ala Gly Trp
50 55 60
His Ser Gly Gly Arg Ala
65 70
<210> 329
<211> 213
<212> DNA
<213> ***链球菌
<400> 329
atgaatacaa ttgaaaaatt tgaaaatatt aaactttttt cactaaagaa aattatcggt 60
ggcaaaactg taaattatgg taatggcctt tattgtaacc aaaaaaaatg ctgggtaaac 120
tggtcagaaa ctgctacaac aatagtaaat aattccatca tgaacgggct cacaggtggt 180
aatgcgggtt ggcactcagg cgggagagca taa 213
<210> 330
<211> 76
<212> PRT
<213> ***链球菌
<400> 330
Met Asp Ile Leu Leu Glu Leu Ala Gly Tyr Thr Gly Ile Ala Ser Gly
1 5 10 15
Thr Ala Lys Lys Val Val Asp Ala Ile Asp Lys Gly Ala Ala Ala Phe
20 25 30
Val Ile Ile Ser Ile Ile Ser Thr Val Ile Ser Ala Gly Ala Leu Gly
35 40 45
Ala Val Ser Ala Ser Ala Asp Phe Ile Ile Leu Thr Val Lys Asn Tyr
50 55 60
Ile Ser Arg Asn Leu Lys Ala Gln Ala Val Ile Trp
65 70 75
<210> 331
<211> 231
<212> DNA
<213> ***链球菌
<400> 331
atggacattt tattagaact cgcaggatat actgggatag cctcaggtac tgcaaaaaaa 60
gttgttgatg ccattgataa aggagctgca gcctttgtta ttatttcaat tatctcaaca 120
gtaattagtg cgggagcatt gggagcagtt tcagcctcag ctgattttat tattttaact 180
gtaaaaaatt acattagtag aaatttaaaa gcacaagctg tcatttggta a 231
<210> 332
<211> 64
<212> PRT
<213> 产气荚膜梭菌
<400> 332
Met Asp Ser Glu Leu Phe Lys Leu Met Ala Thr Gln Gly Ala Phe Ala
1 5 10 15
Ile Leu Phe Ser Tyr Leu Leu Phe Tyr Val Leu Lys Glu Asn Ser Lys
20 25 30
Arg Glu Asp Lys Tyr Gln Asn Ile Ile Glu Glu Leu Thr Glu Leu Leu
35 40 45
Pro Lys Ile Lys Glu Asp Val Glu Asp Ile Lys Glu Lys Leu Asn Lys
50 55 60
<210> 333
<211> 195
<212> DNA
<213> 产气荚膜梭菌
<400> 333
atggatagtg aattatttaa gttaatggca acacaaggag cctttgcaat attattttcg 60
tatttattgt tttatgtttt aaaagagaat agtaaaagag aagataagta tcaaaatata 120
atagaggagc ttacagaatt attgccaaaa ataaaagaag atgtagaaga tataaaagaa 180
aaacttaata aatag 195
<210> 334
<211> 47
<212> PRT
<213> 易变微球菌
<400> 334
Met Thr Asn Ala Phe Gln Ala Leu Asp Glu Val Thr Asp Ala Glu Leu
1 5 10 15
Asp Ala Ile Leu Gly Gly Gly Ser Gly Val Ile Pro Thr Ile Ser His
20 25 30
Glu Cys His Met Asn Ser Phe Gln Phe Val Phe Thr Cys Cys Ser
35 40 45
<210> 335
<211> 144
<212> DNA
<213> 易变微球菌
<400> 335
atgacgaacg catttcaggc actggacgaa gtcacggacg ccgagctcga cgccatcctt 60
ggcgggggca gtggtgttat tcccacgatc agccacgagt gccacatgaa ctccttccag 120
ttcgtgttca cctgctgctc ctga 144
<210> 336
<211> 285
<212> PRT
<213> 马链球菌兽疫亚种
<400> 336
Met Lys Arg Ile Phe Phe Ala Phe Leu Ser Leu Cys Leu Phe Ile Phe
1 5 10 15
Gly Thr Gln Thr Val Ser Ala Ala Thr Tyr Thr Arg Pro Leu Asp Thr
20 25 30
Gly Asn Ile Thr Thr Gly Phe Asn Gly Tyr Pro Gly His Val Gly Val
35 40 45
Asp Tyr Ala Val Pro Val Gly Thr Pro Val Arg Ala Val Ala Asn Gly
50 55 60
Thr Val Lys Phe Ala Gly Asn Gly Ala Asn His Pro Trp Met Leu Trp
65 70 75 80
Met Ala Gly Asn Cys Val Leu Ile Gln His Ala Asp Gly Met His Thr
85 90 95
Gly Tyr Ala His Leu Ser Lys Ile Ser Val Ser Thr Asp Ser Thr Val
100 105 110
Lys Gln Gly Gln Ile Ile Gly Tyr Thr Gly Ala Thr Gly Gln Val Thr
115 120 125
Gly Pro His Leu His Phe Glu Met Leu Pro Ala Asn Pro Asn Trp Gln
130 135 140
Asn Gly Phe Ser Gly Arg Ile Asp Pro Thr Gly Tyr Ile Ala Asn Ala
145 150 155 160
Pro Val Phe Asn Gly Thr Thr Pro Thr Glu Pro Thr Thr Pro Thr Thr
165 170 175
Asn Leu Lys Ile Tyr Lys Val Asp Asp Leu Gln Lys Ile Asn Gly Ile
180 185 190
Trp Gln Val Arg Asn Asn Ile Leu Val Pro Thr Asp Phe Thr Trp Val
195 200 205
Asp Asn Gly Ile Ala Ala Asp Asp Val Ile Glu Val Thr Ser Asn Gly
210 215 220
Thr Arg Thr Ser Asp Gln Val Leu Gln Lys Gly Gly Tyr Phe Val Ile
225 230 235 240
Asn Pro Asn Asn Val Lys Ser Val Gly Thr Pro Met Lys Gly Ser Gly
245 250 255
Gly Leu Ser Trp Ala Gln Val Asn Phe Thr Thr Gly Gly Asn Val Trp
260 265 270
Leu Asn Thr Thr Ser Lys Asp Asn Leu Leu Tyr Gly Lys
275 280 285
<210> 337
<211> 858
<212> DNA
<213> 马链球菌兽疫亚种
<400> 337
atgaaacgta tattttttgc tttcttaagt ttatgcttat ttatattcgg aacacaaacg 60
gtatctgcag ctacttatac tcggccatta gatacgggaa atatcactac agggtttaac 120
ggataccctg gtcatgttgg agtcgattat gcagtacccg ttggaactcc ggttagagca 180
gttgcaaatg gtacagtcaa atttgcaggt aatggggcta atcacccatg gatgctttgg 240
atggctggaa actgtgttct aattcaacat gctgacggga tgcatactgg atatgcacac 300
ttatcaaaaa tttcagttag cacagatagt acagttaaac aaggacaaat cataggttat 360
actggtgcca ccggccaagt taccggtcca catttgcatt ttgaaatgtt gccagcaaat 420
cctaactggc aaaatggttt ttctggaaga atagatccaa ccggatacat cgctaatgcc 480
cctgtattta atggaacaac acctacagaa cctactactc ctacaacaaa tttaaaaatc 540
tataaagttg atgatttaca aaaaattaat ggtatttggc aagtaagaaa taacatactt 600
gtaccaactg atttcacatg ggttgataat ggaattgcag cagatgatgt aattgaagta 660
actagcaatg gaacaagaac ctctgaccaa gttcttcaaa aaggtggtta ttttgtcatc 720
aatcctaata atgttaaaag tgttggaact ccgatgaaag gtagtggtgg tctatcttgg 780
gctcaagtaa actttacaac aggtggaaat gtctggttaa atactactag caaagacaac 840
ttactttacg gaaaataa 858
<210> 338
<211> 45
<212> PRT
<213> 黄褐粘球菌
<400> 338
Ala Asn Cys Ser Cys Ser Thr Ala Ser Asp Tyr Cys Pro Ile Leu Thr
1 5 10 15
Phe Cys Thr Thr Gly Thr Ala Cys Ser Tyr Thr Pro Thr Gly Cys Gly
20 25 30
Thr Gly Trp Val Tyr Cys Ala Cys Asn Gly Asn Phe Tyr
35 40 45
<210> 339
<211> 135
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：338的多肽的多核苷酸
<400> 339
gcgaactgca gctgcagcac cgcgagcgat tattgcccga ttctgacctt ttgcaccacc 60
ggcaccgcgt gcagctatac cccgaccggc tgcggcaccg gctgggtgta ttgcgcgtgc 120
aacggcaact tttat 135
<210> 340
<211> 19
<212> PRT
<213> 灰链霉菌
<400> 340
Cys Ala Asn Ser Cys Ser Tyr Gly Pro Leu Thr Trp Ser Cys Asp Gly
1 5 10 15
Asn Thr Lys
<210> 341
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：340的多肽的多核苷酸
<400> 341
tgcgcgaaca gctgcagcta tggcccgctg acctggagct gcgatggcaa caccaaa 57
<210> 342
<211> 19
<212> PRT
<213> 灰轮丝链轮丝菌
<400> 342
Cys Lys Gln Ser Cys Ser Phe Gly Pro Phe Thr Phe Val Cys Asp Gly
1 5 10 15
Asn Thr Lys
<210> 343
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：342的多肽的多核苷酸
<400> 343
tgcaaacaga gctgcagctt tggcccgttt acctttgtgt gcgatggcaa caccaaa 57
<210> 344
<211> 7
<212> PRT
<213> 肉杆菌
<400> 344
Gly Ser Glu Ile Gln Pro Arg
1 5
<210> 345
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：344的多肽的多核苷酸
<400> 345
ggcagcgaaa ttcagccgcg c 21
<210> 346
<211> 39
<212> PRT
<213> 乳酸乳球菌乳酸亚种
<400> 346
Gly Thr Trp Asp Asp Ile Gly Gln Gly Ile Gly Arg Val Ala Tyr Trp
1 5 10 15
Val Gly Lys Ala Met Gly Asn Met Ser Asp Val Asn Gln Ala Ser Arg
20 25 30
Ile Asn Arg Lys Lys Lys His
35
<210> 347
<211> 117
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：346的多肽的多核苷酸
<400> 347
ggcacctggg atgatattgg ccagggcatt ggccgcgtgg cgtattgggt gggcaaagcg 60
atgggcaaca tgagcgatgt gaaccaggcg agccgcatta accgcaaaaa aaaacat 117
<210> 348
<211> 35
<212> PRT
<213> 乳酸乳球菌乳酸亚种
<400> 348
Lys Lys Trp Gly Trp Leu Ala Trp Val Asp Pro Ala Tyr Glu Phe Ile
1 5 10 15
Lys Gly Phe Gly Lys Gly Ala Ile Lys Glu Gly Asn Lys Asp Lys Trp
20 25 30
Lys Asn Ile
35
<210> 349
<211> 105
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：348的多肽的多核苷酸
<400> 349
aaaaaatggg gctggctggc gtgggtggat ccggcgtatg aatttattaa aggctttggc 60
aaaggcgcga ttaaagaagg caacaaagat aaatggaaaa acatt 105
<210> 350
<211> 19
<212> PRT
<213> 链霉菌属
<400> 350
Cys Val Gln Ser Cys Ser Phe Gly Pro Leu Thr Trp Ser Cys Asp Gly
1 5 10 15
Asn Thr Lys
<210> 351
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：350的多肽的多核苷酸
<400> 351
tgcgtgcaga gctgcagctt tggcccgctg acctggagct gcgatggcaa caccaaa 57
<210> 352
<211> 19
<212> PRT
<213> 利古里亚游动放线菌
<400> 352
Ser Ser Gly Trp Val Cys Thr Leu Thr Ile Glu Cys Gly Thr Val Ile
1 5 10 15
Cys Ala Cys
<210> 353
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：352的多肽的多核苷酸
<400> 353
agcagcggct gggtgtgcac cctgaccatt gaatgcggca ccgtgatttg cgcgtgc 57
<210> 354
<211> 38
<212> PRT
<213> 弯曲乳杆菌
<220>
<221> misc_feature
<222> (34)..(34)
<223> Xaa可以是任何天然氨基酸
<220>
<221> 变体
<222> (37)..(37)
<223> Xaa = 任何氨基酸
<400> 354
Tyr Thr Ala Lys Gln Cys Leu Gln Ala Ile Gly Ser Cys Gly Ile Ala
1 5 10 15
Gly Thr Gly Ala Gly Ala Ala Gly Gly Pro Ala Gly Ala Phe Val Gly
20 25 30
Ala Xaa Val Val Xaa Ile
35
<210> 355
<211> 114
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：354的多肽的多核苷酸
<220>
<221> misc_feature
<222> (100)..(102)
<223> nnn = 任何氨基酸-编码三联体
<220>
<221> misc_feature
<222> (109)..(111)
<223> nnn = 任何氨基酸-编码三联体
<400> 355
tataccgcga aacagtgcct gcaggcgatt ggcagctgcg gcattgcggg caccggcgcg 60
ggcgcggcgg gcggcccggc gggcgcgttt gtgggcgcgn nngtggtgnn natt 114
<210> 356
<211> 42
<212> PRT
<213> 清酒乳杆菌
<220>
<221> 变体
<222> (32)..(32)
<223> Xaa = 任何氨基酸
<400> 356
Thr Lys Tyr Tyr Gly Asn Gly Val Tyr Cys Asn Ser Lys Lys Cys Trp
1 5 10 15
Val Asp Trp Gly Gln Ala Ala Gly Gly Ile Gly Gln Thr Val Val Xaa
20 25 30
Gly Trp Leu Gly Gly Ala Ile Pro Gly Lys
35 40
<210> 357
<211> 126
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：356的多肽的多核苷酸
<220>
<221> misc_feature
<222> (94)..(96)
<223> nnn = 任何氨基酸-编码三联体
<400> 357
accaaatatt atggcaacgg cgtgtattgc aacagcaaaa aatgctgggt ggattggggc 60
caggcggcgg gcggcattgg ccagaccgtg gtgnnnggct ggctgggcgg cgcgattccg 120
ggcaaa 126
<210> 358
<211> 22
<212> PRT
<213> 变形链球菌
<400> 358
Phe Lys Ser Trp Ser Phe Cys Thr Pro Gly Cys Ala Lys Thr Gly Ser
1 5 10 15
Phe Asn Ser Tyr Cys Cys
20
<210> 359
<211> 132
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：358的多肽的多核苷酸
<400> 359
tttaaaagct ggagcttttg caccccgggc tgcgcgaaaa ccggcagctt taacagctat 60
tgctgcttta aaagctggag cttttgcacc ccgggctgcg cgaaaaccgg cagctttaac 120
agctattgct gc 132
<210> 360
<211> 43
<212> PRT
<213> 蒙氏肠球菌
<400> 360
Lys Tyr Tyr Gly Asn Gly Val Ser Cys Asn Lys Lys Gly Cys Ser Val
1 5 10 15
Asp Trp Gly Lys Ala Ile Gly Ile Ile Gly Asn Asn Ser Ala Ala Asn
20 25 30
Leu Ala Thr Gly Gly Ala Ala Gly Trp Ser Lys
35 40
<210> 361
<211> 129
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：360的多肽的多核苷酸
<400> 361
aaatattatg gcaacggcgt gagctgcaac aaaaaaggct gcagcgtgga ttggggcaaa 60
gcgattggca ttattggcaa caacagcgcg gcgaacctgg cgaccggcgg cgcggcgggc 120
tggagcaaa 129
<210> 362
<211> 41
<212> PRT
<213> 清酒乳杆菌
<220>
<221> 变体
<222> (9)..(9)
<223> Xaa = 任何氨基酸
<220>
<221> 变体
<222> (14)..(14)
<223> Xaa = 任何氨基酸
<220>
<221> 变体
<222> (33)..(33)
<223> Xaa = 任何氨基酸
<220>
<221> 变体
<222> (37)..(37)
<223> Xaa = 任何氨基酸
<400> 362
Lys Tyr Tyr Gly Asn Gly Val His Xaa Gly Lys His Ser Xaa Thr Val
1 5 10 15
Asp Trp Gly Thr Ala Ile Gly Asn Ile Gly Asn Asn Ala Ala Ala Asn
20 25 30
Xaa Ala Thr Gly Xaa Asn Ala Gly Gly
35 40
<210> 363
<211> 123
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：362的多肽的多核苷酸
<220>
<221> misc_feature
<222> (25)..(27)
<223> nnn = 任何氨基酸-编码三联体
<220>
<221> misc_feature
<222> (40)..(42)
<223> nnn = 任何氨基酸-编码三联体
<220>
<221> misc_feature
<222> (97)..(99)
<223> nnn = 任何氨基酸-编码三联体
<220>
<221> misc_feature
<222> (109)..(111)
<223> nnn = 任何氨基酸-编码三联体
<400> 363
aaatattatg gcaacggcgt gcatnnnggc aaacatagcn nnaccgtgga ttggggcacc 60
gcgattggca acattggcaa caacgcggcg gcgaacnnng cgaccggcnn naacgcgggc 120
ggc 123
<210> 364
<211> 31
<212> PRT
<213> 副干酪乳杆菌
<400> 364
Gly Met Ser Gly Tyr Ile Gln Gly Ile Pro Asp Phe Leu Lys Gly Tyr
1 5 10 15
Leu His Gly Ile Ser Ala Ala Asn Lys His Lys Lys Gly Arg Leu
20 25 30
<210> 365
<211> 93
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：364的多肽的多核苷酸
<400> 365
ggcatgagcg gctatattca gggcattccg gattttctga aaggctatct gcatggcatt 60
agcgcggcga acaaacataa aaaaggccgc ctg 93
<210> 366
<211> 31
<212> PRT
<213> 肠膜明串珠菌
<400> 366
Lys Gly Lys Gly Phe Trp Ser Trp Ala Ser Lys Ala Thr Ser Trp Leu
1 5 10 15
Thr Gly Pro Gln Gln Pro Gly Ser Pro Leu Leu Lys Lys His Arg
20 25 30
<210> 367
<211> 93
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：366的多肽的多核苷酸
<400> 367
aaaggcaaag gcttttggag ctgggcgagc aaagcgacca gctggctgac cggcccgcag 60
cagccgggca gcccgctgct gaaaaaacat cgc 93
<210> 368
<211> 43
<212> PRT
<213> 肠膜明串珠菌
<400> 368
Lys Asn Tyr Gly Asn Gly Val His Cys Thr Lys Lys Gly Cys Ser Val
1 5 10 15
Asp Trp Gly Tyr Ala Trp Thr Asn Ile Ala Asn Asn Ser Val Met Asn
20 25 30
Gly Leu Thr Gly Gly Asn Ala Gly Trp His Asn
35 40
<210> 369
<211> 129
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：368的多肽的多核苷酸
<400> 369
aaaaactatg gcaacggcgt gcattgcacc aaaaaaggct gcagcgtgga ttggggctat 60
gcgtggacca acattgcgaa caacagcgtg atgaacggcc tgaccggcgg caacgcgggc 120
tggcataac 129
<210> 370
<211> 47
<212> PRT
<213> 枯草芽孢杆菌
<400> 370
Ala Ile Lys Leu Val Gln Ser Pro Asn Gly Asn Phe Ala Ala Ser Phe
1 5 10 15
Val Leu Asp Gly Thr Lys Trp Ile Phe Lys Ser Lys Tyr Tyr Asp Ser
20 25 30
Ser Lys Gly Tyr Trp Val Gly Ile Tyr Glu Val Trp Asp Arg Lys
35 40 45
<210> 371
<211> 141
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：370的多肽的多核苷酸
<400> 371
gcgattaaac tggtgcagag cccgaacggc aactttgcgg cgagctttgt gctggatggc 60
accaaatgga tttttaaaag caaatattat gatagcagca aaggctattg ggtgggcatt 120
tatgaagtgt gggatcgcaa a 141
<210> 372
<211> 12
<212> PRT
<213> 地衣芽孢杆菌
<220>
<221> 变体
<222> (7)..(7)
<223> Xaa = 任何氨基酸
<400> 372
Ile Ser Leu Glu Ile Cys Xaa Ile Phe His Asp Asn
1 5 10
<210> 373
<211> 36
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：372的多肽的多核苷酸
<220>
<221> misc_feature
<222> (19)..(21)
<223> nnn = 任何氨基酸-编码三联体
<400> 373
attagcctgg aaatttgcnn natttttcat gataac 36
<210> 374
<211> 37
<212> PRT
<213> 乳酸乳球菌乳酸亚种
<400> 374
Thr Ser Tyr Gly Asn Gly Val His Cys Asn Lys Ser Lys Cys Trp Ile
1 5 10 15
Asp Val Ser Glu Leu Glu Thr Tyr Lys Ala Gly Thr Val Ser Asn Pro
20 25 30
Lys Asp Ile Leu Trp
35
<210> 375
<211> 111
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：374的多肽的多核苷酸
<400> 375
accagctatg gcaacggcgt gcattgcaac aaaagcaaat gctggattga tgtgagcgaa 60
ctggaaacct ataaagcggg caccgtgagc aacccgaaag atattctgtg g 111
<210> 376
<211> 9
<212> PRT
<213> 沙雷氏杆菌
<400> 376
Asp Tyr His His Gly Val Arg Val Leu
1 5
<210> 377
<211> 27
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：376的多肽的多核苷酸
<400> 377
gattatcatc atggcgtgcg cgtgctg 27
<210> 378
<211> 15
<212> PRT
<213> 嗜盐杆菌
<400> 378
Asp Ile Asp Ile Thr Gly Cys Ser Ala Cys Lys Tyr Ala Ala Gly
1 5 10 15
<210> 379
<211> 45
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：378的多肽的多核苷酸
<400> 379
gatattgata ttaccggctg cagcgcgtgc aaatatgcgg cgggc 45
<210> 380
<211> 12
<212> PRT
<213> 枯草芽孢杆菌
<220>
<221> 变体
<222> (1)..(1)
<223> Xaa = 任何氨基酸
<220>
<221> 变体
<222> (2)..(2)
<223> Xaa = 任何氨基酸
<220>
<221> 变体
<222> (6)..(6)
<223> Xaa = 任何氨基酸
<400> 380
Xaa Xaa Lys Glu Ile Xaa His Ile Phe His Asp Asn
1 5 10
<210> 381
<211> 36
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：380的多肽的多核苷酸
<220>
<221> misc_feature
<222> (1)..(3)
<223> nnn = 任何氨基酸-编码三联体
<220>
<221> misc_feature
<222> (4)..(6)
<223> nnn = 任何氨基酸-编码三联体
<220>
<221> misc_feature
<222> (16)..(18)
<223> nnn = 任何氨基酸-编码三联体
<400> 381
nnnnnnaaag aaattnnnca tatttttcat gataac 36
<210> 382
<211> 12
<212> PRT
<213> 弯曲乳杆菌
<400> 382
Thr Pro Val Val Asn Pro Pro Phe Leu Gln Gln Thr
1 5 10
<210> 383
<211> 36
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：382的多肽的多核苷酸
<400> 383
accccggtgg tgaacccgcc gtttctgcag cagacc 36
<210> 384
<211> 10
<212> PRT
<213> 弯曲乳杆菌
<220>
<221> 变体
<222> (10)..(10)
<223> Xaa = 任何氨基酸
<400> 384
Val Ala Pro Phe Pro Glu Gln Phe Leu Xaa
1 5 10
<210> 385
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：384的多肽的多核苷酸
<220>
<221> misc_feature
<222> (28)..(30)
<223> nnn = 任何氨基酸-编码三联体
<400> 385
gtggcgccgt ttccggaaca gtttctgnnn 30
<210> 386
<211> 9
<212> PRT
<213> 弯曲乳杆菌
<400> 386
Asn Ile Pro Gln Leu Thr Pro Thr Pro
1 5
<210> 387
<211> 27
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：386的多肽的多核苷酸
<400> 387
aacattccgc agctgacccc gaccccg 27
<210> 388
<211> 18
<212> PRT
<213> 苏云金芽孢杆菌杀虫亚种
<220>
<221> 变体
<222> (4)..(4)
<223> Xaa = 任何氨基酸
<220>
<221> 变体
<222> (7)..(7)
<223> Xaa = 任何氨基酸
<220>
<221> 变体
<222> (10)..(10)
<223> Xaa = 任何氨基酸
<400> 388
Asp Trp Thr Xaa Trp Ser Xaa Leu Val Xaa Ala Ala Cys Ser Val Glu
1 5 10 15
Leu Leu
<210> 389
<211> 54
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：388的多肽的多核苷酸
<220>
<221> misc_feature
<222> (10)..(12)
<223> nnn = 任何氨基酸-编码三联体
<220>
<221> misc_feature
<222> (19)..(21)
<223> nnn = 任何氨基酸-编码三联体
<220>
<221> misc_feature
<222> (28)..(30)
<223> nnn = 任何氨基酸-编码三联体
<400> 389
gattggaccn nntggagcnn nctggtgnnn gcggcgtgca gcgtggaact gctg 54
<210> 390
<211> 30
<212> PRT
<213> 弯曲乳杆菌
<400> 390
Ala Tyr Pro Gly Asn Gly Val His Cys Gly Lys Tyr Ser Cys Thr Val
1 5 10 15
Asp Lys Gln Thr Ala Ile Gly Asn Ile Gly Asn Asn Ala Ala
20 25 30
<210> 391
<211> 90
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：390的多肽的多核苷酸
<400> 391
gcgtatccgg gcaacggcgt gcattgcggc aaatatagct gcaccgtgga taaacagacc 60
gcgattggca acattggcaa caacgcggcg 90
<210> 392
<211> 43
<212> PRT
<213> 分歧食肉杆菌
<400> 392
Thr Lys Tyr Tyr Gly Asn Gly Val Tyr Cys Asn Ser Lys Lys Cys Trp
1 5 10 15
Val Asp Trp Gly Thr Ala Gln Gly Cys Ile Asp Val Val Ile Gly Gln
20 25 30
Leu Gly Gly Gly Ile Pro Gly Lys Gly Lys Cys
35 40
<210> 393
<211> 129
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：392的多肽的多核苷酸
<400> 393
accaaatatt atggcaacgg cgtgtattgc aacagcaaaa aatgctgggt ggattggggc 60
accgcgcagg gctgcattga tgtggtgatt ggccagctgg gcggcggcat tccgggcaaa 120
ggcaaatgc 129
<210> 394
<211> 62
<212> PRT
<213> 肠球菌
<400> 394
Asn Arg Trp Tyr Cys Asn Ser Ala Ala Gly Gly Val Gly Gly Ala Ala
1 5 10 15
Val Cys Gly Leu Ala Gly Tyr Val Gly Glu Ala Lys Glu Asn Ile Ala
20 25 30
Gly Glu Val Arg Lys Gly Trp Gly Met Ala Gly Gly Phe Thr His Asn
35 40 45
Lys Ala Cys Lys Ser Phe Pro Gly Ser Gly Trp Ala Ser Gly
50 55 60
<210> 395
<211> 186
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：394的多肽的多核苷酸
<400> 395
aaccgctggt attgcaacag cgcggcgggc ggcgtgggcg gcgcggcggt gtgcggcctg 60
gcgggctatg tgggcgaagc gaaagaaaac attgcgggcg aagtgcgcaa aggctggggc 120
atggcgggcg gctttaccca taacaaagcg tgcaaaagct ttccgggcag cggctgggcg 180
agcggc 186
<210> 396
<211> 39
<212> PRT
<213> 屎肠球菌
<400> 396
Thr Thr Lys Asn Tyr Gly Asn Gly Val Cys Asn Ser Val Asn Trp Cys
1 5 10 15
Gln Cys Gly Asn Val Trp Ala Ser Cys Asn Leu Ala Thr Gly Cys Ala
20 25 30
Ala Trp Leu Cys Lys Leu Ala
35
<210> 397
<211> 117
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：396的多肽的多核苷酸
<400> 397
accaccaaaa actatggcaa cggcgtgtgc aacagcgtga actggtgcca gtgcggcaac 60
gtgtgggcga gctgcaacct ggcgaccggc tgcgcggcgt ggctgtgcaa actggcg 117
<210> 398
<211> 30
<212> PRT
<213> 多粘类芽孢杆菌
<400> 398
Ala Ser Ile Ile Lys Thr Thr Ile Lys Val Ser Lys Ala Val Cys Lys
1 5 10 15
Thr Leu Thr Cys Ile Cys Thr Gly Ser Cys Ser Asn Cys Lys
20 25 30
<210> 399
<211> 90
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：398的多肽的多核苷酸
<400> 399
gcgagcatta ttaaaaccac cattaaagtg agcaaagcgg tgtgcaaaac cctgacctgc 60
atttgcaccg gcagctgcag caactgcaaa 90
<210> 400
<211> 31
<212> PRT
<213> 表皮葡萄球菌
<400> 400
Ser Ala Ser Ile Val Lys Thr Thr Ile Lys Ala Ser Lys Lys Leu Cys
1 5 10 15
Arg Gly Phe Thr Leu Thr Cys Gly Cys His Phe Thr Gly Lys Lys
20 25 30
<210> 401
<211> 93
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：400的多肽的多核苷酸
<400> 401
agcgcgagca ttgtgaaaac caccattaaa gcgagcaaaa aactgtgccg cggctttacc 60
ctgacctgcg gctgccattt taccggcaaa aaa 93
<210> 402
<211> 43
<212> PRT
<213> 屎肠球菌
<400> 402
Lys Tyr Tyr Gly Asn Gly Val Ser Cys Asn Lys Lys Gly Cys Ser Val
1 5 10 15
Asp Trp Gly Lys Ala Ile Gly Ile Ile Gly Asn Asn Ala Ala Ala Asn
20 25 30
Leu Thr Thr Gly Gly Lys Ala Ala Trp Ala Cys
35 40
<210> 403
<211> 129
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：402的多肽的多核苷酸
<400> 403
aaatattatg gcaacggcgt gagctgcaac aaaaaaggct gcagcgtgga ttggggcaaa 60
gcgattggca ttattggcaa caacgcggcg gcgaacctga ccaccggcgg caaagcggcg 120
tgggcgtgc 129
<210> 404
<211> 39
<212> PRT
<213> 多粘类芽孢杆菌
<400> 404
Ala Thr Tyr Tyr Gly Asn Gly Leu Tyr Cys Asn Lys Gln Lys His Tyr
1 5 10 15
Thr Trp Val Asp Trp Asn Lys Ala Ser Arg Glu Ile Gly Lys Ile Thr
20 25 30
Val Asn Gly Trp Val Gln His
35
<210> 405
<211> 117
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：404的多肽的多核苷酸
<400> 405
gcgacctatt atggcaacgg cctgtattgc aacaaacaga aacattatac ctgggtggat 60
tggaacaaag cgagccgcga aattggcaaa attaccgtga acggctgggt gcagcat 117
<210> 406
<211> 35
<212> PRT
<213> 环状芽胞杆菌
<400> 406
Val Asn Tyr Gly Asn Gly Val Ser Cys Ser Lys Thr Lys Cys Ser Val
1 5 10 15
Asn Trp Gly Ile Ile Thr His Gln Ala Phe Arg Val Thr Ser Gly Val
20 25 30
Ala Ser Gly
35
<210> 407
<211> 105
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：406的多肽的多核苷酸
<400> 407
gtgaactatg gcaacggcgt gagctgcagc aaaaccaaat gcagcgtgaa ctggggcatt 60
attacccatc aggcgtttcg cgtgaccagc ggcgtggcga gcggc 105
<210> 408
<211> 30
<212> PRT
<213> 多粘类芽孢杆菌
<400> 408
Phe Val Tyr Gly Asn Gly Val Thr Ser Ile Leu Val Gln Ala Gln Phe
1 5 10 15
Leu Val Asn Gly Gln Arg Arg Phe Phe Tyr Thr Pro Asp Lys
20 25 30
<210> 409
<211> 90
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：408的多肽的多核苷酸
<400> 409
tttgtgtatg gcaacggcgt gaccagcatt ctggtgcagg cgcagtttct ggtgaacggc 60
cagcgccgct ttttttatac cccggataaa 90
<210> 410
<211> 13
<212> PRT
<213> 鼠李糖乳杆菌
<400> 410
Ala Val Pro Ala Val Arg Lys Thr Asn Glu Thr Leu Asp
1 5 10
<210> 411
<211> 39
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：410的多肽的多核苷酸
<400> 411
gcggtgccgg cggtgcgcaa aaccaacgaa accctggat 39
<210> 412
<211> 69
<212> PRT
<213> 地衣芽孢杆菌
<400> 412
Met Lys Asn Ser Ala Ala Arg Glu Ala Phe Lys Gly Ala Asn His Pro
1 5 10 15
Ala Gly Met Val Ser Glu Glu Glu Leu Lys Ala Leu Val Gly Gly Asn
20 25 30
Asp Val Asn Pro Glu Thr Thr Pro Ala Thr Thr Ser Ser Trp Thr Cys
35 40 45
Ile Thr Ala Gly Val Thr Val Ser Ala Ser Leu Cys Pro Thr Thr Lys
50 55 60
Cys Thr Ser Arg Cys
65
<210> 413
<211> 207
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：412的多肽的多核苷酸
<400> 413
atgaaaaaca gcgcggcgcg cgaagcgttt aaaggcgcga accatccggc gggcatggtg 60
agcgaagaag aactgaaagc gctggtgggc ggcaacgatg tgaacccgga aaccaccccg 120
gcgaccacca gcagctggac ctgcattacc gcgggcgtga ccgtgagcgc gagcctgtgc 180
ccgaccacca aatgcaccag ccgctgc 207
<210> 414
<211> 36
<212> PRT
<213> 植物乳杆菌
<400> 414
Lys Tyr Tyr Gly Asn Gly Leu Ser Cys Ser Lys Lys Gly Cys Thr Val
1 5 10 15
Asn Trp Gly Gln Ala Phe Ser Cys Gly Val Asn Arg Val Ala Thr Ala
20 25 30
Gly His Gly Lys
35
<210> 415
<211> 108
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：414的多肽的多核苷酸
<400> 415
aaatattatg gcaacggcct gagctgcagc aaaaaaggct gcaccgtgaa ctggggccag 60
gcgtttagct gcggcgtgaa ccgcgtggcg accgcgggcc atggcaaa 108
<210> 416
<211> 24
<212> PRT
<213> 嗜酸乳杆菌
<400> 416
Gly Asn Pro Lys Val Ala His Cys Ala Ser Gln Ile Gly Arg Ser Thr
1 5 10 15
Ala Trp Gly Ala Val Ser Gly Ala
20
<210> 417
<211> 72
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：416的多肽的多核苷酸
<400> 417
ggcaacccga aagtggcgca ttgcgcgagc cagattggcc gcagcaccgc gtggggcgcg 60
gtgagcggcg cg 72
<210> 418
<211> 40
<212> PRT
<213> 粪肠球菌
<400> 418
Trp Leu Pro Pro Ala Gly Leu Leu Gly Arg Cys Gly Arg Trp Phe Arg
1 5 10 15
Pro Trp Leu Leu Trp Leu Gln Ser Gly Ala Gln Tyr Lys Trp Leu Gly
20 25 30
Asn Leu Phe Gly Leu Gly Pro Lys
35 40
<210> 419
<211> 120
<212> DNA
<213> 人工序列
<220>
<223> 编码SEQ ID NO：418的多肽的多核苷酸
<400> 419
tggctgccgc cggcgggcct gctgggccgc tgcggccgct ggtttcgccc gtggctgctg 60
tggctgcaga gcggcgcgca gtataaatgg ctgggcaacc tgtttggcct gggcccgaaa 120
<210> 420
<211> 40
<212> PRT
<213> 多变鱼腥藻
<400> 420
Asn Leu Asp Gln Trp Leu Thr Glu Gln Val His Glu Phe Gln Asp Met
1 5 10 15
Tyr Leu Glu Pro Gln Ala Ile Ser Asn Gln Asp Ile Thr Phe Lys Leu
20 25 30
Ser Asp Leu Asp Phe Ile His Asn
35 40
<210> 421
<211> 124
<212> DNA
<213> 多变鱼腥藻
<400> 421
taatttagat cagtggttaa cagaacaagt tcatgagttt caagatatgt acttggaacc 60
acaagcaata tccaatcaag acattacctt caaactatct gacctagatt ttattcataa 120
ttga 124
<210> 422
<211> 40
<212> PRT
<213> 念珠藻
<400> 422
Asn Leu Asp Gln Trp Leu Thr Glu Gln Val His Glu Phe Gln Asp Met
1 5 10 15
Tyr Leu Glu Pro Gln Ala Ile Ser Asn Gln Asp Ile Thr Phe Lys Leu
20 25 30
Ser Asp Leu Asp Phe Ile His Asn
35 40
<210> 423
<211> 123
<212> DNA
<213> 念珠藻
<400> 423
aatttagatc aatggttaac agaacaagtt catgagtttc aagatatgta cttggaacca 60
caagcaatat ccaatcaaga cattaccttc aaactgtcag acctagattt tattcataat 120
tga 123
<210> 424
<211> 7
<212> PRT
<213> 满江红念珠藻
<400> 424
His Arg Glu Lys Lys Ser Ala
1 5
<210> 425
<211> 24
<212> DNA
<213> 满江红念珠藻
<400> 425
cacagagaga aaaaatcagc atag 24
<210> 426
<211> 23
<212> PRT
<213> Acaryochloris marina
<400> 426
Thr Ser Asn Asn Trp Leu Ala Lys Asn Tyr Leu Ser Met Trp Asn Lys
1 5 10 15
Lys Ser Ser Asn Pro Asn Leu
20
<210> 427
<211> 72
<212> DNA
<213> Acaryochloris marina
<400> 427
acaagcaata actggctagc caaaaactat ctttctatgt ggaataaaaa gagcagtaat 60
ccaaaccttt ag 72
<210> 428
<211> 6
<212> PRT
<213> 蓝丝菌
<400> 428
Phe Arg Tyr Phe Trp Trp
1 5
<210> 429
<211> 21
<212> DNA
<213> 蓝丝菌
<400> 429
tttagatatt tttggtggta a 21
<210> 430
<211> 6
<212> PRT
<213> 蓝丝菌
<400> 430
Phe Arg Tyr Phe Trp Trp
1 5
<210> 431
<211> 21
<212> DNA
<213> 蓝丝菌
<400> 431
tttagatatt tttggtggta a 21
<210> 432
<211> 9
<212> PRT
<213> 蓝丝菌
<400> 432
Cys Gly Glu Lys Trp Arg Ile Phe Ser
1 5
<210> 433
<211> 27
<212> DNA
<213> 蓝丝菌
<400> 433
tgtggagaaa aatggagaat ttttagc 27
<210> 434
<211> 8
<212> PRT
<213> 蓝丝菌
<400> 434
Phe Arg Leu Gln Leu Trp Gln Phe
1 5
<210> 435
<211> 24
<212> DNA
<213> 蓝丝菌
<400> 435
tttcgcttac aactgtggca attt 24
<210> 436
<211> 16
<212> PRT
<213> 蓝丝菌
<400> 436
Leu Gly Cys Asn Gln Ser Ser Ile Trp Ser Ile Phe Phe Trp Asn His
1 5 10 15
<210> 437
<211> 51
<212> DNA
<213> 蓝丝菌
<400> 437
ctaggatgta accagagcag tatctggtca atttttttct ggaatcatta a 51
<210> 438
<211> 40
<212> PRT
<213> 原型微鞘藻
<400> 438
Tyr Asn Leu Gln Gly Leu Pro Ala Ile Glu Ser Glu Asp Cys Ile Pro
1 5 10 15
Asp Ser Val Ala Pro Ser Asp Asp Trp Phe Ser Gly Val Ser Ser Leu
20 25 30
Phe Asn Arg Leu Thr Gly Leu Gly
35 40
<210> 439
<211> 123
<212> DNA
<213> 原型微鞘藻
<400> 439
tataacctac aggggttgcc agcaattgag tcagaagact gtatcccaga ttctgtagcg 60
ccttcggatg attggttttc aggcgtatcg tctctgttta accgcttgac tgggttgggt 120
tag 123
<210> 440
<211> 37
<212> PRT
<213> 念珠藻
<400> 440
Trp Met Ala Ile Arg Arg Ile Leu Arg Cys His Pro Phe His Pro Gly
1 5 10 15
Gly Tyr Asp Pro Val Pro Glu Leu Gly Glu His Cys Cys His His Asp
20 25 30
Ser Gly Asn Lys Gly
35
<210> 441
<211> 114
<212> DNA
<213> 念珠藻
<400> 441
tggatggcga ttcgccgcat tttgcgttgt catccattcc acccaggggg ttatgatcct 60
gtaccagagt tgggtgagca ttgttgtcat catgatagcg ggaataaggg gtga 114
<210> 442
<211> 35
<212> PRT
<213> 多变鱼腥藻
<400> 442
Trp Met Gly Ile Arg Arg Ile Leu Arg Cys His Pro Phe His Pro Gly
1 5 10 15
Gly Tyr Asp Pro Val Pro Glu Val Gly Glu His Cys Cys His His Asp
20 25 30
Ser Gly Lys
35
<210> 443
<211> 108
<212> DNA
<213> 多变鱼腥藻
<400> 443
tggatgggga ttcgccgcat tttgcgttgt catccattcc acccaggcgg ttatgatcct 60
gtaccagagg tgggtgagca ttgttgtcat catgatagcg ggaagtag 108
<210> 444
<211> 48
<212> PRT
<213> 泡沫节球藻
<400> 444
Trp Met Ala Thr Arg Arg Ile Leu Arg Cys His Pro Phe His Pro Gly
1 5 10 15
Gly Tyr Asp Pro Val Pro Glu Val Lys His Asn Cys Cys Asp Gln His
20 25 30
Leu Ser Asp Ser Gly Lys Gln Thr Thr Glu Asp His His Lys Gly Ser
35 40 45
<210> 445
<211> 147
<212> DNA
<213> 泡沫节球藻
<400> 445
tggatggcga ctcggcggat tttgcgttgt catcccttcc atcctggtgg atatgatcca 60
gttccagagg taaaacacaa ttgctgcgat cagcatctgt ccgattctgg gaaacagacc 120
acagaagacc atcacaaagg ctcgtag 147
<210> 446
<211> 34
<212> PRT
<213> 满江红念珠藻
<400> 446
Trp Met Ala Thr Leu Arg Ile Leu Arg Cys His Pro Phe His Pro Gly
1 5 10 15
Gly Tyr Asp Pro Val Pro Gly Leu Ala Glu Lys Ser Cys Cys Asp His
20 25 30
His Asp
<210> 447
<211> 105
<212> DNA
<213> 满江红念珠藻
<400> 447
tggatggcaa ctttgcggat tttacgctgt catcctttcc atcctggtgg ttatgatcct 60
gtaccaggac tagcggaaaa atcctgttgt gaccatcatg attga 105
<210> 448
<211> 28
<212> PRT
<213> 聚球藻
<400> 448
Trp Leu Thr Ala Lys Arg Phe Cys Arg Cys His Pro Leu His Pro Gly
1 5 10 15
Gly Tyr Asp Pro Val Pro Glu Lys Lys Ser Val Leu
20 25
<210> 449
<211> 87
<212> DNA
<213> 聚球藻
<400> 449
tggctaacag ccaagcgctt ttgtcgctgt catccgcttc atcctggcgg gtatgatccg 60
gtaccggaga agaaatcggt actctaa 87
<210> 450
<211> 23
<212> PRT
<213> 原绿球藻
<400> 450
Trp Leu Thr Leu Arg Arg Leu Ser Arg Cys His Pro Phe Thr Pro Cys
1 5 10 15
Gly Cys Asp Pro Val Pro Asp
20
<210> 451
<211> 72
<212> DNA
<213> 原绿球藻
<400> 451
tggctcaccc tgcggcgcct gtctcgttgc catcctttta ccccctgtgg ttgcgacccg 60
gtgcctgatt aa 72
<210> 452
<211> 69
<212> PRT
<213> 大肠杆菌
<400> 452
Met Ser Tyr Lys Lys Leu Tyr Gln Leu Thr Ala Ile Phe Ser Leu Pro
1 5 10 15
Leu Thr Ile Leu Leu Val Ser Leu Ser Ser Leu Arg Ile Val Gly Glu
20 25 30
Gly Asn Ser Tyr Val Asp Val Phe Leu Ser Phe Ile Ile Phe Leu Gly
35 40 45
Phe Ile Glu Leu Ile His Gly Ile Arg Lys Ile Leu Val Trp Ser Gly
50 55 60
Trp Lys Asn Gly Ser
65
<210> 453
<211> 210
<212> DNA
<213> 大肠杆菌
<400> 453
atgagttata aaaaactgta ccaattgacg gctatattta gtttacctct tactatctta 60
ttggtttcac tttcatccct tcggattgtt ggcgaaggga attcttatgt tgacgttttt 120
ctaagcttta taatatttct tggttttatt gagctgattc atgggattcg aaagattttg 180
gtctggtcag gctggaaaaa cggaagttaa 210
<210> 454
<211> 85
<212> PRT
<213> 大肠杆菌
<400> 454
Met Gly Leu Lys Leu Asp Leu Thr Trp Phe Asp Lys Ser Thr Glu Asp
1 5 10 15
Phe Lys Gly Glu Glu Tyr Ser Lys Asp Phe Gly Asp Asp Gly Ser Val
20 25 30
Met Glu Ser Leu Gly Val Pro Phe Lys Asp Asn Val Asn Asn Gly Cys
35 40 45
Phe Asp Val Ile Ala Glu Trp Val Pro Leu Leu Gln Pro Tyr Phe Asn
50 55 60
His Gln Ile Asp Ile Ser Asp Asn Glu Tyr Phe Val Ser Phe Asp Tyr
65 70 75 80
Arg Asp Gly Asp Trp
85
<210> 455
<211> 258
<212> DNA
<213> 大肠杆菌
<400> 455
atgggactta aattggattt aacttggttt gataaaagta cagaagattt taagggtgag 60
gagtattcaa aagattttgg agatgacggt tcagttatgg aaagtctagg tgtgcctttt 120
aaggataatg ttaataacgg ttgctttgat gttatagctg aatgggtacc tttgctacaa 180
ccatacttta atcatcaaat tgatatttcc gataatgagt attttgtttc gtttgattat 240
cgtgatggtg attggtga 258
<210> 456
<211> 113
<212> PRT
<213> 大肠杆菌
<400> 456
Met Ser Leu Arg Tyr Tyr Ile Lys Asn Ile Leu Phe Gly Leu Tyr Cys
1 5 10 15
Thr Leu Ile Tyr Ile Tyr Leu Ile Thr Lys Asn Ser Glu Gly Tyr Tyr
20 25 30
Phe Leu Val Ser Asp Lys Met Leu Tyr Ala Ile Val Ile Ser Thr Ile
35 40 45
Leu Cys Pro Tyr Ser Lys Tyr Ala Ile Glu Tyr Ile Ala Phe Asn Phe
50 55 60
Ile Lys Lys Asp Phe Phe Glu Arg Arg Lys Asn Leu Asn Asn Ala Pro
65 70 75 80
Val Ala Lys Leu Asn Leu Phe Met Leu Tyr Asn Leu Leu Cys Leu Val
85 90 95
Leu Ala Ile Pro Phe Gly Leu Leu Gly Leu Phe Ile Ser Ile Lys Asn
100 105 110
Asn
<210> 457
<211> 342
<212> DNA
<213> 大肠杆菌
<400> 457
atgagcttaa gatactacat aaaaaatatt ttatttggcc tgtactgcac acttatatat 60
atatacctta taacaaaaaa cagcgaaggg tattatttcc ttgtgtcaga taagatgcta 120
tatgcaatag tgataagcac tattctatgt ccatattcaa aatatgctat tgaatacata 180
gcttttaact tcataaagaa agattttttc gaaagaagaa aaaacctaaa taacgccccc 240
gtagcaaaat taaacctatt tatgctatat aatctacttt gtttggtcct agcaatccca 300
tttggattgc taggactttt tatatcaata aagaataatt aa 342
<210> 458
<211> 85
<212> PRT
<213> 大肠杆菌
<400> 458
Met Gly Leu Lys Leu His Ile His Trp Phe Asp Lys Lys Thr Glu Glu
1 5 10 15
Phe Lys Gly Gly Glu Tyr Ser Lys Asp Phe Gly Asp Asp Gly Ser Val
20 25 30
Ile Glu Ser Leu Gly Met Pro Leu Lys Asp Asn Ile Asn Asn Gly Trp
35 40 45
Phe Asp Val Glu Lys Pro Trp Val Ser Ile Leu Gln Pro His Phe Lys
50 55 60
Asn Val Ile Asp Ile Ser Lys Phe Asp Tyr Phe Val Ser Phe Val Tyr
65 70 75 80
Arg Asp Gly Asn Trp
85
<210> 459
<211> 258
<212> DNA
<213> 大肠杆菌
<400> 459
atggggctta aattacatat tcattggttt gataagaaaa ccgaagagtt taaaggcggt 60
gaatactcaa aagacttcgg tgatgatggt tctgtcattg aaagtctggg gatgccttta 120
aaggataata ttaataatgg ttggtttgat gttgaaaaac catgggtttc gatattacag 180
ccacacttta aaaatgtaat cgatattagt aaatttgatt actttgtatc ctttgtttac 240
cgggatggta actggtaa 258
<210> 460
<211> 86
<212> PRT
<213> 大肠杆菌
<400> 460
Met Glu Leu Lys His Ser Ile Ser Asp Tyr Thr Glu Ala Glu Phe Leu
1 5 10 15
Glu Phe Val Lys Lys Ile Cys Arg Ala Glu Gly Ala Thr Glu Glu Asp
20 25 30
Asp Asn Lys Leu Val Arg Glu Phe Glu Arg Leu Thr Glu His Pro Asp
35 40 45
Gly Ser Asp Leu Ile Tyr Tyr Pro Arg Asp Asp Arg Glu Asp Ser Pro
50 55 60
Glu Gly Ile Val Lys Glu Ile Lys Glu Trp Arg Ala Ala Asn Gly Lys
65 70 75 80
Ser Gly Phe Lys Gln Gly
85
<210> 461
<211> 261
<212> DNA
<213> 大肠杆菌
<400> 461
atggaactga aacatagtat tagtgattat accgaggctg aatttctgga gtttgtaaaa 60
aaaatatgta gagctgaagg tgctactgaa gaggatgaca ataaattagt gagagagttt 120
gagcgattaa ctgagcaccc agatggttca gatctgattt attatcctcg cgatgacagg 180
gaagatagtc ctgaagggat tgtcaaggaa attaaagaat ggcgagctgc taacggtaag 240
tcaggattta aacagggctg a 261
<210> 462
<211> 178
<212> PRT
<213> 弗氏柠檬酸杆菌
<400> 462
Met Met Asn Glu His Ser Ile Asp Thr Asp Asn Arg Lys Ala Asn Asn
1 5 10 15
Ala Leu Tyr Leu Phe Ile Ile Ile Gly Leu Ile Pro Leu Leu Cys Ile
20 25 30
Phe Val Val Tyr Tyr Lys Thr Pro Asp Ala Leu Leu Leu Arg Lys Ile
35 40 45
Ala Thr Ser Thr Glu Asn Leu Pro Ser Ile Thr Ser Ser Tyr Asn Pro
50 55 60
Leu Met Thr Lys Val Met Asp Ile Tyr Cys Lys Thr Ala Pro Phe Leu
65 70 75 80
Ala Leu Ile Leu Tyr Ile Leu Thr Phe Lys Ile Arg Lys Leu Ile Asn
85 90 95
Asn Thr Asp Arg Asn Thr Val Leu Arg Ser Cys Leu Leu Ser Pro Leu
100 105 110
Val Tyr Ala Ala Ile Val Tyr Leu Phe Cys Phe Arg Asn Phe Glu Leu
115 120 125
Thr Thr Ala Gly Arg Pro Val Arg Leu Met Ala Thr Asn Asp Ala Thr
130 135 140
Leu Leu Leu Phe Tyr Ile Gly Leu Tyr Ser Ile Ile Phe Phe Thr Thr
145 150 155 160
Tyr Ile Thr Leu Phe Thr Pro Val Thr Ala Phe Lys Leu Leu Lys Lys
165 170 175
Arg Gln
<210> 463
<211> 537
<212> DNA
<213> 弗氏柠檬酸杆菌
<400> 463
atgatgaatg aacactcaat agatacggac aacagaaagg ccaataacgc attgtattta 60
tttataataa tcggattaat accattattg tgcatttttg ttgtttacta caaaacgcca 120
gacgctttac ttttacgtaa aattgctaca agcactgaga atctcccgtc aataacatcc 180
tcctacaacc cattaatgac aaaggttatg gatatttatt gtaaaacagc gcctttcctt 240
gccttaatac tatacatcct aacctttaaa atcagaaaat taatcaacaa caccgacagg 300
aacactgtac ttagatcttg tttattaagt ccattggtct atgcagcaat tgtttatcta 360
ttctgcttcc gaaattttga gttaacaaca gccggaaggc ctgtcagatt aatggccacc 420
aatgacgcaa cactattgtt attttatatt ggtctgtact caataatttt ctttacaacc 480
tatatcacgc tattcacacc agtcactgca tttaaattat taaaaaaaag gcagtaa 537
<210> 464
<211> 111
<212> PRT
<213> 大肠杆菌
<400> 464
Met Asn Arg Lys Tyr Tyr Phe Asn Asn Met Trp Trp Gly Trp Val Thr
1 5 10 15
Gly Gly Tyr Met Leu Tyr Met Ser Trp Asp Tyr Glu Phe Lys Tyr Arg
20 25 30
Leu Leu Phe Trp Cys Ile Ser Leu Cys Gly Met Val Leu Tyr Pro Val
35 40 45
Ala Lys Trp Tyr Ile Glu Asp Thr Ala Leu Lys Phe Thr Arg Pro Asp
50 55 60
Phe Trp Asn Ser Gly Phe Phe Ala Asp Thr Pro Gly Lys Met Gly Leu
65 70 75 80
Leu Ala Val Tyr Thr Gly Thr Val Phe Ile Leu Ser Leu Pro Leu Ser
85 90 95
Met Ile Tyr Ile Leu Ser Val Ile Ile Lys Arg Leu Ser Val Arg
100 105 110
<210> 465
<211> 336
<212> DNA
<213> 大肠杆菌
<400> 465
atgaacagaa aatattattt taataatatg tggtggggat gggtgacggg gggatatatg 60
ctgtatatgt catgggatta tgagtttaaa tacagattac tgttctggtg tatttctctc 120
tgcggaatgg ttttgtatcc ggttgcaaaa tggtatattg aagatacagc tctaaaattt 180
acccggcctg atttctggaa cagcggtttt tttgctgata cacctggaaa aatggggttg 240
cttgcggttt atacgggtac tgttttcata ttatctcttc cgttaagtat gatatatatt 300
ctttctgtta ttataaaaag gctgtctgta agatag 336
<210> 466
<211> 115
<212> PRT
<213> 大肠杆菌
<400> 466
Met Lys Leu Asp Ile Ser Val Lys Tyr Leu Leu Lys Ser Leu Ile Pro
1 5 10 15
Ile Leu Ile Ile Leu Thr Val Phe Tyr Leu Gly Trp Lys Asp Asn Gln
20 25 30
Glu Asn Ala Arg Met Phe Tyr Ala Phe Ile Gly Cys Ile Ile Ser Ala
35 40 45
Ile Thr Phe Pro Phe Ser Met Arg Ile Ile Gln Lys Met Val Ile Arg
50 55 60
Phe Thr Gly Lys Glu Phe Trp Gln Lys Asp Phe Phe Thr Asn Pro Val
65 70 75 80
Gly Gly Ser Leu Thr Ala Ile Phe Glu Leu Phe Cys Phe Val Ile Ser
85 90 95
Val Pro Val Val Ala Ile Tyr Leu Ile Phe Ile Leu Cys Lys Ala Leu
100 105 110
Ser Gly Lys
115
<210> 467
<211> 348
<212> DNA
<213> 大肠杆菌
<400> 467
atgaaactgg atatatctgt aaagtattta ctgaaaagcc tgataccaat cctcattatt 60
cttacagttt tttatctggg atggaaagat aaccaggaaa atgcaagaat gttttatgcg 120
ttcatcggat gcattatcag tgccattact tttccttttt caatgaggat aatacagaaa 180
atggtaataa ggtttacagg gaaagaattc tggcaaaaag acttctttac aaatccagtt 240
ggcggaagct taactgcaat atttgaatta ttctgtttcg ttatatcagt tcctgtggtt 300
gccatttact taatttttat actctgcaaa gccctttcag gaaaatga 348
<210> 468
<211> 131
<212> PRT
<213> 大肠杆菌
<400> 468
Met His Asn Thr Leu Leu Glu Lys Ile Ile Ala Tyr Leu Ser Leu Pro
1 5 10 15
Gly Phe His Ser Leu Asn Asn Pro Pro Leu Ser Glu Ala Phe Asn Leu
20 25 30
Tyr Val His Thr Ala Pro Leu Ala Ala Thr Ser Leu Phe Ile Phe Thr
35 40 45
His Lys Glu Leu Glu Leu Lys Pro Lys Ser Ser Pro Leu Arg Ala Leu
50 55 60
Lys Ile Leu Thr Pro Phe Thr Ile Leu Tyr Ile Ser Met Ile Tyr Cys
65 70 75 80
Phe Leu Leu Thr Asp Thr Glu Leu Thr Leu Ser Ser Lys Thr Phe Val
85 90 95
Leu Ile Val Lys Lys Arg Ser Val Phe Val Phe Phe Leu Tyr Asn Thr
100 105 110
Ile Tyr Trp Asp Ile Tyr Ile His Ile Phe Val Leu Leu Val Pro Tyr
115 120 125
Arg Asn Ile
130
<210> 469
<211> 396
<212> DNA
<213> 大肠杆菌
<400> 469
atgcacaata cactcctcga aaaaatcatc gcatacctat ccctaccagg atttcattca 60
ttaaacaacc cgcccctaag cgaagcattc aatctctatg ttcatacagc ccctttagct 120
gcaaccagct tattcatatt cacacacaaa gaattagagt taaaaccaaa gtcgtcacct 180
ctgcgggcac taaagatatt aactcctttc actattcttt atatatccat gatatactgt 240
ttcttgctaa ctgacacaga actaaccttg tcatcaaaaa catttgtatt aatagtcaaa 300
aaacgatctg tttttgtctt ttttctatat aacactatat attgggatat atatattcac 360
atatttgtac ttttggttcc ttataggaac atataa 396
<210> 470
<211> 85
<212> PRT
<213> 大肠杆菌
<400> 470
Met Glu Leu Lys Asn Ser Ile Ser Asp Tyr Thr Glu Thr Glu Phe Lys
1 5 10 15
Lys Ile Ile Glu Asp Ile Ile Asn Cys Glu Gly Asp Glu Lys Lys Gln
20 25 30
Asp Asp Asn Leu Glu His Phe Ile Ser Val Thr Glu His Pro Ser Gly
35 40 45
Ser Asp Leu Ile Tyr Tyr Pro Glu Gly Asn Asn Asp Gly Ser Pro Glu
50 55 60
Ala Val Ile Lys Glu Ile Lys Glu Trp Arg Ala Ala Asn Gly Lys Ser
65 70 75 80
Gly Phe Lys Gln Gly
85
<210> 471
<211> 258
<212> DNA
<213> 大肠杆菌
<400> 471
atggaactga aaaacagcat tagtgattac actgaaactg aattcaaaaa aattattgaa 60
gacatcatca attgtgaagg tgatgaaaaa aaacaggatg ataacctcga gcattttata 120
agtgttactg agcatcctag tggttctgat ctgatttatt acccagaagg taataatgat 180
ggtagccctg aagctgttat taaagagatt aaagaatggc gagctgctaa cggtaagtca 240
ggatttaaac agggctga 258
<210> 472
<211> 98
<212> PRT
<213> 乳酸乳球菌乳酸亚种
<400> 472
Met Lys Lys Lys Gln Ile Glu Phe Glu Asn Glu Leu Arg Ser Met Leu
1 5 10 15
Ala Thr Ala Leu Glu Lys Asp Ile Ser Gln Glu Glu Arg Asn Ala Leu
20 25 30
Asn Ile Ala Glu Lys Ala Leu Asp Asn Ser Glu Tyr Leu Pro Lys Ile
35 40 45
Ile Leu Asn Leu Arg Lys Ala Leu Thr Pro Leu Ala Ile Asn Arg Thr
50 55 60
Leu Asn His Asp Leu Ser Glu Leu Tyr Lys Phe Ile Thr Ser Ser Lys
65 70 75 80
Ala Ser Asn Lys Asn Leu Gly Gly Gly Leu Ile Met Ser Trp Gly Arg
85 90 95
Leu Phe
<210> 473
<211> 297
<212> DNA
<213> 乳酸乳球菌乳酸亚种
<400> 473
atgaaaaaaa aacaaataga atttgaaaac gagctaagaa gtatgttggc taccgccctt 60
gaaaaagaca ttagtcaaga ggaaagaaat gctctgaata ttgcagaaaa ggcgcttgac 120
aattctgaat atttaccaaa aattatttta aacctcagaa aagccctaac tccattagct 180
ataaatcgaa cacttaacca tgatttatct gaactgtata aattcattac aagttccaaa 240
gcatcaaaca aaaatttagg tggtggttta attatgtcgt ggggacgact attctaa 297
<210> 474
<211> 98
<212> PRT
<213> 乳酸乳球菌乳脂亚种
<400> 474
Met Lys Lys Lys Gln Ile Glu Phe Glu Asn Glu Leu Arg Ser Met Leu
1 5 10 15
Ala Thr Ala Leu Glu Lys Asp Ile Ser Gln Glu Glu Arg Asn Ala Leu
20 25 30
Asn Ile Ala Glu Lys Ala Leu Asp Asn Ser Glu Tyr Leu Pro Lys Ile
35 40 45
Ile Leu Asn Leu Arg Lys Ala Leu Thr Pro Leu Ala Ile Asn Arg Thr
50 55 60
Leu Asn His Asp Leu Ser Glu Leu Tyr Lys Phe Ile Thr Ser Ser Lys
65 70 75 80
Ala Ser Asn Lys Asn Leu Gly Gly Gly Leu Ile Met Ser Trp Gly Arg
85 90 95
Leu Phe
<210> 475
<211> 297
<212> DNA
<213> 乳酸乳球菌乳脂亚种
<400> 475
atgaaaaaaa aacaaataga atttgaaaac gagctaagaa gtatgttggc taccgccctt 60
gaaaaagaca ttagtcaaga ggaaagaaat gctctgaata ttgcagaaaa ggcgcttgac 120
aattctgaat atttaccaaa aattatttta aacctcagaa aagccctaac tccattagct 180
ataaatcgaa cacttaacca tgatttatct gaactgtata aattcattac aagttccaaa 240
gcatcaaaca aaaatttagg tggtggttta attatgtcgt ggggacgact attctaa 297
<210> 476
<211> 87
<212> PRT
<213> 大肠杆菌
<400> 476
Met Asn Lys Met Ala Met Ile Asp Leu Ala Lys Leu Phe Leu Ala Ser
1 5 10 15
Lys Ile Thr Ala Ile Glu Phe Ser Glu Arg Ile Cys Val Glu Arg Arg
20 25 30
Arg Leu Tyr Gly Val Lys Asp Leu Ser Pro Asn Ile Leu Asn Cys Gly
35 40 45
Glu Glu Leu Phe Met Ala Ala Glu Arg Phe Glu Pro Asp Ala Asp Arg
50 55 60
Ala Asn Tyr Glu Ile Asp Asp Asn Gly Leu Lys Val Glu Val Arg Ser
65 70 75 80
Ile Leu Glu Lys Phe Lys Leu
85
<210> 477
<211> 249
<212> DNA
<213> 大肠杆菌
<400> 477
atgatcgatt tggcgaaatt atttttagct tcgaaaatta cagtgattga gttttcagag 60
cgaatttgtg ttgaacggag aagattgtat ggtgttaagg atttgtctcc gaatatatta 120
aattgtgggg aagagttgtc tatggctgct gagcgatttg agcctgatgc agatagggct 180
aattatgaaa ttgatgataa tggacttaag gtcgaggtcc gatctatctt ggaaaaactt 240
aaatcataa 249
<210> 478
<211> 83
<212> PRT
<213> 大肠杆菌
<400> 478
Met Lys Leu Ser Pro Lys Ala Ala Ile Glu Val Cys Asn Glu Ala Ala
1 5 10 15
Lys Lys Gly Leu Trp Ile Leu Gly Ile Asp Gly Gly His Trp Leu Asn
20 25 30
Pro Gly Phe Arg Ile Asp Ser Ser Ala Ser Trp Thr Tyr Asp Met Pro
35 40 45
Glu Glu Tyr Lys Ser Lys Ile Pro Glu Asn Asn Arg Leu Ala Ile Glu
50 55 60
Asn Ile Lys Asp Asp Ile Glu Asn Gly Tyr Thr Ala Phe Ile Ile Thr
65 70 75 80
Leu Lys Met
<210> 479
<211> 243
<212> DNA
<213> 大肠杆菌
<400> 479
atgaagttat caccaaaagc tgcaatagaa gtttgtaatg aagcagcgaa aaaaggctta 60
tggattttgg gcattgatgg tgggcattgg ctgaatcctg gattcaggat agatagttca 120
gcatcatgga catatgatat gccggagaat acaaatcaaa aatccctgaa aataatagat 180
tggctattga aaatattaaa gatgatattg agaatggata cactgctttc attatcacgt 240
taa 243
<210> 480
<211> 85
<212> PRT
<213> 大肠杆菌
<400> 480
Met Gly Leu Lys Leu His Ile Asn Trp Phe Asp Lys Arg Thr Glu Glu
1 5 10 15
Phe Lys Gly Gly Glu Tyr Ser Lys Asp Phe Gly Asp Asp Gly Ser Val
20 25 30
Ile Glu Arg Leu Gly Met Pro Phe Lys Asp Asn Ile Asn Asn Gly Trp
35 40 45
Phe Asp Val Ile Ala Glu Trp Val Pro Leu Leu Gln Pro Tyr Phe Asn
50 55 60
His Gln Ile Asp Ile Ser Asp Asn Glu Tyr Phe Val Ser Phe Asp Tyr
65 70 75 80
Arg Asp Gly Asp Trp
85
<210> 481
<211> 258
<212> DNA
<213> 大肠杆菌
<400> 481
atggggctta aattacatat taattggttt gataagacga ccgaggaatt taaaggtggt 60
gagtattcaa aagattttgg agatgatggc tcggtcattg aacgtcttgg aatgccttta 120
aaagataata tcaataatgg ttggtttgat gttatagctg aatgggtacc tttgctacaa 180
ccatacttta atcatcaaat tgatatttcc gataatgagt attttgtttc gtttgattat 240
cgtgatggtg attggtga 258
<210> 482
<211> 85
<212> PRT
<213> 大肠杆菌
<400> 482
Met Glu Leu Lys Lys Ser Ile Gly Asp Tyr Thr Glu Thr Glu Phe Lys
1 5 10 15
Lys Ile Ile Glu Asn Ile Ile Asn Cys Glu Gly Asp Glu Lys Lys Gln
20 25 30
Asp Asp Asn Leu Glu His Phe Ile Ser Val Thr Glu His Pro Ser Gly
35 40 45
Ser Asp Leu Ile Tyr Tyr Pro Glu Gly Asn Asn Asp Gly Ser Pro Glu
50 55 60
Ala Val Ile Lys Glu Ile Lys Glu Trp Arg Ala Ala Asn Gly Lys Ser
65 70 75 80
Gly Phe Lys Gln Gly
85
<210> 483
<211> 258
<212> DNA
<213> 大肠杆菌
<400> 483
gtggagctaa agaaaagtat tggtgattac actgaaaccg aattcaaaaa aattattgaa 60
aacatcatca attgtgaagg tgatgaaaaa aaacaggatg ataacctcga gcattttata 120
agtgttactg agcatcctag tggttctgat ctgatttatt acccagaagg taataatgat 180
ggtagccctg aagctgttat taaagagatt aaagaatggc gagctgctaa cggtaagtca 240
ggatttaaac agggctga 258
<210> 484
<211> 86
<212> PRT
<213> 大肠杆菌
<400> 484
Met Glu Leu Lys His Ser Ile Ser Asp Tyr Thr Glu Ala Glu Phe Leu
1 5 10 15
Gln Leu Val Thr Thr Ile Cys Asn Ala Asp Thr Ser Ser Glu Glu Glu
20 25 30
Leu Val Lys Leu Val Thr His Phe Glu Glu Met Thr Glu His Pro Ser
35 40 45
Gly Ser Asp Leu Ile Tyr Tyr Pro Lys Glu Gly Asp Asp Asp Ser Pro
50 55 60
Ser Gly Ile Val Asn Thr Val Lys Gln Trp Arg Ala Ala Asn Gly Lys
65 70 75 80
Ser Gly Phe Lys Gln Gly
85
<210> 485
<211> 261
<212> DNA
<213> 大肠杆菌
<400> 485
atggaactga agcatagcat tagtgattat acagaagctg aatttttaca acttgtaaca 60
acaatttgta atgcgaacac ttccagtgaa gaagaactgg ttaaattggt tacacacttt 120
gaggaaatga ctgagcaccc tagtggtagt gatttaatat attacccaaa agaaggtgat 180
gatgactcac cttcaggtat tgtaaacaca gtaaaacaat ggcgagccgc taacggtaag 240
tcaggattta aacagggcta a 261
<210> 486
<211> 141
<212> PRT
<213> 大肠杆菌
<400> 486
Met Leu Thr Leu Tyr Gly Tyr Ile Arg Asn Val Phe Leu Tyr Arg Met
1 5 10 15
Asn Asp Arg Ser Cys Gly Asp Phe Met Lys Val Ile Ser Met Lys Phe
20 25 30
Ile Phe Ile Leu Thr Ile Ile Ala Leu Ala Ala Val Phe Phe Trp Ser
35 40 45
Glu Asp Lys Gly Pro Ala Cys Tyr Gln Val Ser Asp Glu Gln Ala Arg
50 55 60
Thr Phe Val Lys Asn Asp Tyr Leu Gln Arg Met Lys Arg Trp Asp Asn
65 70 75 80
Asp Val Gln Leu Leu Gly Thr Glu Ile Pro Lys Ile Thr Trp Glu Lys
85 90 95
Ile Glu Arg Ser Leu Thr Asp Val Glu Asp Glu Lys Thr Leu Leu Val
100 105 110
Pro Phe Lys Ala Glu Gly Pro Asp Gly Lys Arg Met Tyr Tyr Gly Met
115 120 125
Tyr His Cys Glu Glu Gly Tyr Val Glu Tyr Ala Asn Asp
130 135 140
<210> 487
<211> 354
<212> DNA
<213> 大肠杆菌
<400> 487
atgaaagtaa ttagcatgaa atttattttt attttaacga ttattgctct tgctgctgtt 60
tttttctggt ctgaagataa aggtccggca tgctatcagg tcagcgatga acaggccaga 120
acgtttgtaa aaaatgatta cctgcaaaga atgaaacgct gggacaacga tgtacaactt 180
cttggtacag aaatcccgaa aattacatgg gaaaagattg agagaagttt aacagatgtt 240
gaagatgaaa aaacacttct tgtcccattt aaagctgaag gcccggacgg taagagaatg 300
tattatggca tgtaccattg tgaggaggga tatgttgaat atgcgaatga ctaa 354
<210> 488
<211> 175
<212> PRT
<213> 大肠杆菌
<400> 488
Met Thr Ser Asn Lys Asp Lys Asn Lys Lys Ala Asn Glu Ile Leu Tyr
1 5 10 15
Ala Phe Ser Ile Ile Gly Ile Ile Pro Leu Met Ala Ile Leu Ile Leu
20 25 30
Arg Ile Asn Asp Pro Tyr Ser Gln Val Leu Tyr Tyr Leu Tyr Asn Lys
35 40 45
Val Ala Phe Leu Pro Ser Ile Thr Ser Leu His Asp Pro Val Met Thr
50 55 60
Thr Leu Met Ser Asn Tyr Asn Lys Thr Ala Pro Val Met Gly Ile Leu
65 70 75 80
Val Phe Leu Cys Thr Tyr Lys Thr Arg Glu Ile Ile Lys Pro Val Thr
85 90 95
Arg Lys Leu Val Val Gln Ser Cys Phe Trp Gly Pro Val Phe Tyr Ala
100 105 110
Ile Leu Ile Tyr Ile Thr Leu Phe Tyr Asn Leu Glu Leu Thr Thr Ala
115 120 125
Gly Gly Phe Phe Lys Leu Leu Ser His Asn Val Ile Thr Leu Phe Ile
130 135 140
Leu Tyr Cys Ser Ile Tyr Phe Thr Val Leu Thr Met Thr Tyr Ala Ile
145 150 155 160
Leu Leu Met Pro Leu Leu Val Ile Lys Tyr Phe Lys Gly Arg Gln
165 170 175
<210> 489
<211> 528
<212> DNA
<213> 大肠杆菌
<400> 489
atgaccagca ataaagataa gaacaagaaa gcaaacgaaa tattatatgc attttccata 60
atcgggatta ttccattaat ggctatatta atacttcgaa taaatgatcc atattctcaa 120
gtgctgtact acttatataa taaggtggca tttctccctt ctattacatc attgcatgat 180
cccgtcatga caacacttat gtcaaactac aacaagacag cgccagttat gggtattctc 240
gtttttcttt gcacatataa gacaagagaa atcataaagc cagtaacaag aaaacttgtt 300
gtgcaatcct gtttctgggg gcccgttttt tatgccattc tgatttatat cacactgttc 360
tataatctgg aactaacaac agcaggtggt ttttttaaat tattatctca taatgtcatc 420
actctgttta ttttatattg ctccatttac tttactgttt taaccatgac atatgcgatt 480
ttactgatgc cattacttgt cattaaatat tttaaaggga ggcagtaa 528
<210> 490
<211> 78
<212> PRT
<213> 大肠杆菌
<400> 490
Met Asp Arg Lys Arg Thr Lys Leu Glu Leu Leu Phe Ala Phe Ile Ile
1 5 10 15
Asn Ala Thr Ala Ile Tyr Ile Ala Leu Ala Ile Tyr Asp Cys Val Phe
20 25 30
Arg Gly Lys Asp Phe Leu Ser Met His Thr Phe Cys Phe Ser Ala Leu
35 40 45
Met Ser Ala Ile Cys Tyr Phe Val Gly Asp Asn Tyr Tyr Ser Ile Ser
50 55 60
Asp Lys Ile Lys Arg Arg Ser Tyr Glu Asn Ser Asp Ser Lys
65 70 75
<210> 491
<211> 237
<212> DNA
<213> 大肠杆菌
<400> 491
atggatagaa aaagaacaaa attagagttg ttatttgcat ttataataaa tgccaccgca 60
atatatattg cattagctat atatgattgt gtttttagag gaaaggactt tttatccatg 120
catacatttt gcttctctgc attaatgtct gcaatatgtt actttgttgg tgataattat 180
tattcaatat ccgataagat aaaaaggaga tcatatgaga actctgactc taaatga 237
<210> 492
<211> 113
<212> PRT
<213> 宋内志贺氏菌
<400> 492
Met Ser Leu Arg Tyr Tyr Ile Lys Asn Ile Leu Phe Gly Leu Tyr Cys
1 5 10 15
Ala Leu Ile Tyr Ile Tyr Leu Ile Thr Lys Asn Asn Glu Gly Tyr Tyr
20 25 30
Phe Leu Ala Ser Asp Lys Met Leu Tyr Ala Ile Val Ile Ser Thr Ile
35 40 45
Leu Cys Pro Tyr Ser Lys Tyr Ala Ile Glu His Ile Phe Phe Lys Phe
50 55 60
Ile Lys Lys Asp Phe Phe Arg Lys Arg Lys Asn Leu Asn Lys Cys Pro
65 70 75 80
Arg Gly Lys Ile Lys Pro Tyr Leu Cys Val Tyr Asn Leu Leu Cys Leu
85 90 95
Val Leu Ala Ile Pro Phe Gly Leu Leu Gly Leu Val Tyr Ile Asn Lys
100 105 110
Glu
<210> 493
<211> 342
<212> DNA
<213> 宋内志贺氏菌
<400> 493
atgagtttaa gatactacat aaaaaatatt ttgtttggcc tatactgcgc acttatatat 60
atatacctta taacaaaaaa caacgaaggg tattatttcc tagcgtcaga taagatgcta 120
tacgcaatag tgataagcac tattctatgc ccatattcaa aatatgctat tgaacacata 180
ttttttaagt tcataaagaa agattttttc agaaaaagaa aaaacctaaa taaatgcccc 240
cgtggcaaaa ttaaaccgta tttatgcgta tacaatctac tttgtttggt cctagcaatc 300
ccatttggat tgctaggact tgtttatatc aataaagaat aa 342
<210> 494
<211> 113
<212> PRT
<213> 大肠杆菌
<400> 494
Met Ser Leu Arg Tyr Tyr Ile Lys Asn Ile Leu Phe Gly Leu Tyr Cys
1 5 10 15
Thr Leu Ile Tyr Ile Tyr Leu Ile Thr Lys Asn Ser Glu Glu Tyr Tyr
20 25 30
Phe Leu Val Thr Asp Lys Met Leu Tyr Ala Ile Val Ile Ser Thr Ile
35 40 45
Leu Cys Pro Tyr Ser Lys Tyr Ala Ile Glu His Ile Ala Phe Asn Phe
50 55 60
Ile Lys Lys His Phe Phe Glu Arg Arg Lys Asn Leu Asn Asn Ala Pro
65 70 75 80
Val Ala Lys Leu Asn Leu Phe Met Leu Tyr Asn Leu Leu Cys Leu Val
85 90 95
Leu Ala Ile Pro Phe Gly Leu Leu Gly Leu Phe Ile Ser Ile Lys Asn
100 105 110
Asn
<210> 495
<211> 342
<212> DNA
<213> 大肠杆菌
<400> 495
atgagcttaa gatactacat aaaaaatatt ttatttggcc tgtactgcac acttatatat 60
atatacctta taacaaaaaa cagcgaagag tattatttcc ttgtgacaga taagatgcta 120
tatgcaatag tgataagcac tattctatgt ccatattcaa aatatgctat tgaacacata 180
gcttttaact tcataaagaa acattttttc gaaagaagaa aaaacctaaa taacgccccc 240
gtagcaaaat taaacctatt tatgctatat aatctacttt gtttggtcct agcaatccca 300
tttggattgc taggactttt tatatcaata aagaataatt aa 342
<210> 496
<211> 113
<212> PRT
<213> 串珠菌
<400> 496
Met Arg Lys Asn Asn Ile Leu Leu Asp Asp Ala Lys Ile Tyr Thr Asn
1 5 10 15
Lys Leu Tyr Leu Leu Leu Ile Asp Arg Lys Asp Asp Ala Gly Tyr Gly
20 25 30
Asp Ile Cys Asp Val Leu Phe Gln Val Ser Lys Lys Leu Asp Ser Thr
35 40 45
Lys Asn Val Glu Ala Leu Ile Asn Arg Leu Val Asn Tyr Ile Arg Ile
50 55 60
Thr Ala Ser Thr Asn Arg Ile Lys Phe Ser Lys Asp Glu Glu Ala Val
65 70 75 80
Ile Ile Glu Leu Gly Val Ile Gly Gln Lys Ala Gly Leu Asn Gly Gln
85 90 95
Tyr Met Ala Asp Phe Ser Asp Lys Ser Gln Phe Tyr Ser Ile Phe Glu
100 105 110
Arg
<210> 497
<211> 342
<212> DNA
<213> 串珠菌
<400> 497
ttgagaaaaa ataacatttt attggacgat gctaaaatat acacgaacaa actctatttg 60
ctattaatcg atagaaaaga tgacgctggg tatggagata tttgtgatgt tttgtttcag 120
gtatccaaaa aattagatag cacaaaaaat gtagaagcat tgattaaccg attggtcaat 180
tatatacgaa ttaccgcttc aacaaacaga attaagtttt caaaagatga agaggctgta 240
attatagaac ttggtgtaat tggtcagaag gctggattaa acggccaata catggctgat 300
ttttctgaca aatctcagtt ttatagtatc tttgaaagat aa 342
<210> 498
<211> 91
<212> PRT
<213> 乳酸乳球菌乳脂亚种
<400> 498
Met Lys Lys Lys Val Asp Thr Glu Lys Gln Ile Thr Ser Trp Ala Ser
1 5 10 15
Asp Leu Ala Ser Lys Asn Glu Thr Lys Val Gln Glu Lys Leu Ile Leu
20 25 30
Ser Ser Tyr Ile Gln Asp Ile Glu Asn His Val Tyr Phe Pro Lys Ala
35 40 45
Met Ile Ser Leu Glu Lys Lys Leu Arg Asp Gln Asn Asn Ile Cys Ala
50 55 60
Leu Ser Lys Glu Val Asn Gln Phe Tyr Phe Lys Val Val Glu Val Asn
65 70 75 80
Gln Arg Lys Ser Trp Met Val Gly Leu Ile Val
85 90
<210> 499
<211> 276
<212> DNA
<213> 乳酸乳球菌乳脂亚种
<400> 499
atgaaaaaaa aagttgatac agaaaaacaa attacttctt gggcatctga cttagcttcc 60
aaaaatgaaa caaaggttca agaaaaatta atactgtctt cttatattca ggacatcgaa 120
aaccatgttt actttccaaa agcaatgatt tctttagaaa aaaaattacg agaccaaaat 180
aatatttgcg ctttatcaaa agaagtcaat cagttttatt ttaaagttgt tgaagtaaat 240
caaagaaaat cctggatggt aggtttgata gtttaa 276
<210> 500
<211> 112
<212> PRT
<213> 乳酸片球菌
<400> 500
Met Asn Lys Thr Lys Ser Glu His Ile Lys Gln Gln Ala Leu Asp Leu
1 5 10 15
Phe Thr Arg Leu Gln Phe Leu Leu Gln Lys His Asp Thr Ile Glu Pro
20 25 30
Tyr Gln Tyr Val Leu Asp Ile Leu Glu Thr Gly Ile Ser Lys Thr Lys
35 40 45
His Asn Gln Gln Thr Pro Glu Arg Gln Ala Arg Val Val Tyr Asn Lys
50 55 60
Ile Ala Ser Gln Ala Leu Val Asp Lys Leu His Phe Thr Ala Glu Glu
65 70 75 80
Asn Lys Val Leu Ala Ala Ile Asn Glu Leu Ala His Ser Gln Lys Gly
85 90 95
Trp Gly Glu Phe Asn Met Leu Asp Thr Thr Asn Thr Trp Pro Ser Gln
100 105 110
<210> 501
<211> 339
<212> DNA
<213> 乳酸片球菌
<400> 501
atgaataaga ctaagtcgga acatattaaa caacaagctt tggacttatt tactaggcta 60
cagtttttac tacagaagca cgatactatc gaaccttacc agtacgtttt agatattctg 120
gagactggta tcagtaaaac taaacataac cagcaaacgc ctgaacgaca agctcgtgta 180
gtctacaaca agattgccag ccaagcgtta gtagataagt tacattttac tgccgaagaa 240
aacaaagttc tagcagccat caatgaattg gcgcattctc aaaaagggtg gggcgagttt 300
aacatgctag atactaccaa tacgtggcct agccaatag 339
<210> 502
<211> 88
<212> PRT
<213> 麦芽香肉杆菌
<400> 502
Met Ile Lys Asp Glu Lys Ile Asn Lys Ile Tyr Ala Leu Val Lys Ser
1 5 10 15
Ala Leu Asp Asn Thr Asp Val Lys Asn Asp Lys Lys Leu Ser Leu Leu
20 25 30
Leu Met Arg Ile Gln Glu Thr Ser Ile Asn Gly Glu Leu Phe Tyr Asp
35 40 45
Tyr Lys Lys Glu Leu Gln Pro Ala Ile Ser Met Tyr Ser Ile Gln His
50 55 60
Asn Phe Arg Val Pro Asp Asp Leu Val Lys Leu Leu Ala Leu Val Gln
65 70 75 80
Thr Pro Lys Ala Trp Ser Gly Phe
85
<210> 503
<211> 267
<212> DNA
<213> 麦芽香肉杆菌
<400> 503
atgataaaag atgaaaaaat aaataaaatc tatgctttag ttaagagcgc acttgataat 60
acggatgtta agaatgataa aaaactttct ttacttctta tgagaataca agaaacatca 120
attaatggag aactatttta cgattataaa aaagaattac agccagctat tagtatgtac 180
tctattcaac ataactttcg ggttcctgac gatctagtaa aactgttagc attagttcaa 240
acacctaaag cttggtcagg gttttaa 267
<210> 504
<211> 111
<212> PRT
<213> 麦芽香肉杆菌
<400> 504
Met Asp Ile Lys Ser Gln Thr Leu Tyr Leu Asn Leu Ser Glu Ala Tyr
1 5 10 15
Lys Asp Pro Glu Val Lys Ala Asn Glu Phe Leu Ser Lys Leu Val Val
20 25 30
Gln Cys Ala Gly Lys Leu Thr Ala Ser Asn Ser Glu Asn Ser Tyr Ile
35 40 45
Glu Val Ile Ser Leu Leu Ser Arg Gly Ile Ser Ser Tyr Tyr Leu Ser
50 55 60
His Lys Arg Ile Ile Pro Ser Ser Met Leu Thr Ile Tyr Thr Gln Ile
65 70 75 80
Gln Lys Asp Ile Lys Asn Gly Asn Ile Asp Thr Glu Lys Leu Arg Lys
85 90 95
Tyr Glu Ile Ala Lys Gly Leu Met Ser Val Pro Tyr Ile Tyr Phe
100 105 110
<210> 505
<211> 336
<212> DNA
<213> 麦芽香肉杆菌
<400> 505
atggatataa agtctcaaac attatatttg aatctaagcg aggcatataa agaccctgaa 60
gtaaaagcta atgaattctt atcaaaatta gttgtacaat gtgctgggaa attaacagct 120
tcaaacagtg agaacagtta tattgaagta atatcattgc tatctagggg tatttctagt 180
tattatttat cccataaacg tataattcct tcaagtatgt taactatata tactcaaata 240
caaaaggata taaaaaacgg gaatattgac accgaaaaat taaggaaata tgagatagca 300
aaaggattaa tgtccgttcc ttatatatat ttctaa 336
<210> 506
<211> 245
<212> PRT
<213> 乳酸乳球菌乳酸亚种
<400> 506
Met Arg Arg Tyr Leu Ile Leu Ile Val Ala Leu Ile Gly Ile Thr Gly
1 5 10 15
Leu Ser Gly Cys Tyr Gln Thr Ser His Lys Lys Val Arg Phe Asp Glu
20 25 30
Gly Ser Tyr Thr Asn Phe Ile Tyr Asp Asn Lys Ser Tyr Phe Val Thr
35 40 45
Asp Lys Glu Ile Pro Gln Glu Asn Val Asn Asn Ser Lys Val Lys Phe
50 55 60
Tyr Lys Leu Leu Ile Val Asp Met Lys Ser Glu Lys Leu Leu Ser Ser
65 70 75 80
Ser Asn Lys Asn Ser Val Thr Leu Val Leu Asn Asn Ile Tyr Glu Ala
85 90 95
Ser Asp Lys Ser Leu Cys Met Gly Ile Asn Asp Arg Tyr Tyr Lys Ile
100 105 110
Leu Pro Glu Ser Asp Lys Gly Ala Val Lys Ala Leu Arg Leu Gln Asn
115 120 125
Phe Asp Val Thr Ser Asp Ile Ser Asp Asp Asn Phe Val Ile Asp Lys
130 135 140
Asn Asp Ser Arg Lys Ile Asp Tyr Met Gly Asn Ile Tyr Ser Ile Ser
145 150 155 160
Asp Thr Thr Val Ser Asp Glu Glu Leu Gly Glu Tyr Gln Asp Val Leu
165 170 175
Ala Glu Val Arg Val Phe Asp Ser Val Ser Gly Lys Ser Ile Pro Arg
180 185 190
Ser Glu Trp Gly Arg Ile Asp Lys Asp Gly Ser Asn Ser Lys Gln Ser
195 200 205
Arg Thr Glu Trp Asp Tyr Gly Glu Ile His Ser Ile Arg Gly Lys Ser
210 215 220
Leu Thr Glu Ala Phe Ala Val Glu Ile Asn Asp Asp Phe Lys Leu Ala
225 230 235 240
Thr Lys Val Gly Asn
245
<210> 507
<211> 738
<212> DNA
<213> 乳酸乳球菌乳酸亚种
<400> 507
atgagaagat atttaatact tattgtggcc ttaataggga taacaggttt atcagggtgt 60
tatcaaacaa gtcataaaaa ggtgaggttt gacgaaggaa gttatactaa ttttatttat 120
gataataaat cgtatttcgt aactgataag gagattcctc aggagaacgt taacaattcc 180
aaagtaaaat tttataagct gttgattgtt gacatgaaaa gtgagaaact tttatcaagt 240
agcaacaaaa atagtgtgac tttggtctta aataatattt atgaggcttc tgacaagtcg 300
ctatgtatgg gtattaacga cagatactat aagatacttc cagaaagtga taagggggcg 360
gtcaaagctt tgagattaca aaactttgat gtgacaagcg atatttctga tgataatttt 420
gttattgata aaaatgattc acgaaaaatt gactatatgg gaaatattta cagtatatcg 480
gacaccaccg tatctgatga agaattggga gaatatcagg atgttttagc tgaagtacgt 540
gtgtttgatt cagttagtgg caaaagtatc ccgaggtctg aatgggggag aattgataag 600
gatggttcaa attccaaaca gagtaggacg gaatgggatt atggcgaaat ccattctatt 660
agaggaaaat ctcttactga agcatttgcc gttgagataa atgatgattt taagcttgca 720
acgaaggtag gaaactag 738
<210> 508
<211> 261
<212> PRT
<213> 豆科根瘤菌 三叶草
<400> 508
Met Asn Asp Glu Ile Cys Leu Thr Gly Gly Gly Arg Thr Thr Val Thr
1 5 10 15
Arg Arg Gly Gly Val Val Tyr Arg Glu Gly Gly Pro Trp Ser Ser Thr
20 25 30
Val Ile Ser Leu Leu Arg His Leu Glu Ala Ser Gly Phe Ala Glu Ala
35 40 45
Pro Ser Val Val Gly Thr Gly Phe Asp Glu Arg Gly Arg Glu Thr Leu
50 55 60
Ser Phe Ile Glu Gly Glu Phe Val His Pro Gly Pro Trp Ser Glu Glu
65 70 75 80
Ala Phe Pro Gln Phe Gly Met Met Leu Arg Arg Leu His Asp Ala Thr
85 90 95
Ala Ser Phe Lys Pro Pro Glu Asn Ser Met Trp Arg Asp Trp Phe Gly
100 105 110
Arg Asn Leu Gly Glu Gly Gln His Val Ile Gly His Cys Asp Thr Gly
115 120 125
Pro Trp Asn Ile Val Cys Arg Ser Gly Leu Pro Val Gly Leu Ile Asp
130 135 140
Trp Glu Val Ala Gly Pro Val Arg Ala Asp Ile Glu Leu Ala Gln Ala
145 150 155 160
Cys Trp Leu Asn Ala Gln Leu Tyr Asp Asp Asp Ile Ala Glu Arg Val
165 170 175
Gly Leu Gly Ser Val Thr Met Arg Ala His Gln Val Arg Leu Leu Leu
180 185 190
Asp Gly Tyr Gly Leu Ser Arg Lys Gln Arg Gly Gly Phe Val Asp Lys
195 200 205
Leu Ile Thr Phe Ala Val His Asp Ala Ala Glu Gln Ala Lys Glu Ala
210 215 220
Ala Val Thr Pro Glu Ser Asn Asp Ala Glu Pro Leu Trp Ala Ile Ala
225 230 235 240
Trp Arg Thr Arg Ser Ala Ser Trp Met Leu His His Arg Gln Thr Leu
245 250 255
Glu Ala Ala Leu Ala
260
<210> 509
<211> 786
<212> DNA
<213> 豆科根瘤菌 三叶草
<400> 509
atgaatgatg agatttgcct gacaggtggc ggacgaacga ctgtcacgcg gcgcggcgga 60
gtcgtgtatc gcgaaggcgg cccgtggtca tcaaccgtca tttcgctcct gcggcatctg 120
gaagcctctg gcttcgctga agctccttcc gttgtcggca ccggtttcga tgagcgcggc 180
cgggagacat tatcgtttat cgagggtgag tttgttcacc caggcccttg gtcggaggag 240
gcttttccgc aatttggaat gatgttgcgg cgactgcacg atgccaccgc ctcgttcaaa 300
cctcccgaaa actcgatgtg gcgcgattgg ttcgggcgta acctcggtga gggtcaacac 360
gtaataggac actgcgacac aggcccatgg aacattgttt gccggtcagg attgcctgtc 420
gggttgatag attgggaggt ggctgggcct gtcagggcgg atatcgaatt ggcccaggct 480
tgttggctga atgcccagct ctacgatgac gacattgcgg agagggtcgg attaggctct 540
gtgaccatga gagcgcatca agttcgcctg ctgcttgacg gctatggtct gtctcggaag 600
caacgcggcg gcttcgtcga caagctaatc acgttcgcag ttcacgatgc ggccgagcag 660
gcgaaagagg cggctgtcac gccagagtcg aacgatgcgg aaccgctatg ggcaattgcc 720
tggcgcacta gaagtgcctc ctggatgctc catcatcggc aaacactgga agcagcgctg 780
gcatag 786
<210> 510
<211> 436
<212> PRT
<213> 枯草芽孢杆菌
<400> 510
Met Asn Asn Ile Ile Pro Ile Met Ser Leu Leu Phe Lys Gln Leu Tyr
1 5 10 15
Ser Arg Gln Gly Lys Lys Asp Ala Ile Arg Ile Ala Ala Gly Leu Val
20 25 30
Ile Leu Ala Val Phe Glu Ile Gly Leu Ile Arg Gln Ala Gly Ile Asp
35 40 45
Glu Ser Val Leu Arg Lys Thr Tyr Ile Ile Leu Ala Leu Leu Leu Met
50 55 60
Asn Thr Tyr Met Val Phe Leu Ser Val Thr Ser Gln Trp Lys Glu Ser
65 70 75 80
Tyr Met Lys Leu Ser Cys Leu Leu Pro Ile Ser Ser Arg Ser Phe Trp
85 90 95
Leu Ala Gln Ser Val Val Leu Phe Val Asp Thr Cys Leu Arg Arg Thr
100 105 110
Leu Phe Phe Phe Ile Leu Pro Leu Phe Leu Phe Gly Asn Gly Thr Leu
115 120 125
Ser Gly Ala Gln Thr Leu Phe Trp Leu Gly Arg Phe Ser Phe Phe Thr
130 135 140
Val Tyr Ser Ile Ile Phe Gly Val Val Leu Ser Asn His Phe Val Lys
145 150 155 160
Lys Lys Asn Leu Met Phe Leu Leu His Ala Ala Ile Phe Ala Cys Val
165 170 175
Cys Ile Ser Ala Ala Leu Met Pro Ala Ala Thr Ile Pro Leu Cys Ala
180 185 190
Val His Ile Leu Trp Ala Val Val Ile Asp Phe Pro Val Phe Leu Gln
195 200 205
Ala Pro Pro Gln Gln Gly Lys Met His Ser Phe Met Arg Arg Ser Glu
210 215 220
Phe Ser Phe Tyr Lys Arg Glu Trp Asn Arg Phe Ile Ser Ser Lys Ala
225 230 235 240
Met Leu Leu Asn Tyr Ala Val Met Ala Val Phe Ser Gly Phe Phe Ser
245 250 255
Phe Gln Met Met Asn Thr Gly Ile Phe Asn Gln Gln Val Ile Tyr Ile
260 265 270
Val Ile Ser Ala Leu Leu Leu Ile Cys Ser Pro Ile Ala Leu Leu Tyr
275 280 285
Ser Ile Glu Lys Asn Asp Arg Met Leu Leu Ile Thr Leu Pro Ile Lys
290 295 300
Arg Lys Thr Met Phe Trp Ala Lys Tyr Arg Phe Tyr Ser Gly Leu Leu
305 310 315 320
Ala Gly Gly Phe Leu Leu Val Val Met Ile Val Gly Phe Ile Ser Gly
325 330 335
Arg Ser Ile Ser Val Leu Thr Phe Leu Gln Cys Ile Glu Leu Leu Leu
340 345 350
Ala Gly Ala Tyr Ile Arg Leu Thr Ala Asp Glu Lys Arg Pro Ser Phe
355 360 365
Ser Trp Gln Thr Glu Gln Gln Leu Trp Ser Gly Phe Ser Lys Tyr Arg
370 375 380
Ser Tyr Leu Phe Cys Leu Pro Leu Phe Leu Ala Ile Leu Ala Gly Thr
385 390 395 400
Ala Val Ser Leu Ala Val Ile Pro Ile Ala Gly Leu Val Ile Val Tyr
405 410 415
Tyr Leu Gln Lys Gln Asp Gly Gly Phe Phe Asp Thr Ser Lys Arg Glu
420 425 430
Arg Leu Gly Ser
435
<210> 511
<211> 1000
<212> DNA
<213> 枯草芽孢杆菌
<400> 511
atgaataaca taatccctat catgtctttg ctgttcaaac agctttacag ccggcaaggg 60
aaaaaggacg ccatccgcat tgccgcaggc cttgtcattc tggccgtgtt tgaaatcggg 120
ctgatccgcc aggccggcat tgatgaatcg gtgttgcgca aaacgtatat catactcgcg 180
cttcttttga tgaacacata tatggtgttt ctttccgtga catcacaatg gaaggaatct 240
tatatgaagc tgagctgcct gctgccgatt tcttcacgga gcttttggct cgcccagagt 300
gtcgttttgt ttgtcgatac ctgtttgaga agaactttat tcttttttat tttaccgctg 360
ttcttatttg gaaacggaac gctgtcaggg gcgcaaacat tgttttggct cggcaggttt 420
tcgtttttta ccgtttactc cattattttc ggagttgtgc taagcaacca cttcgtcaaa 480
aagaagaact tgatgtttct gctgcatgcg gcgatattcg cctgtgtatg tatcagcgcc 540
gctttgatgc cggccgccac gattccgctt tgcgcggttc atatcctgtg ggcggtggtc 600
attgactttc ctgtctttct gcaggcgcct ccgcagcagg gcaagatgca ttcatttatg 660
cggcgatctg aattttcgtt ttacaaaaga gaatggaacc gatttatctc ttctaaagcg 720
atgctgttaa attacgcggt aatggcggta ttcagcggct tcttttcgtt ccagatgatg 780
aacaccggca tcttcaatca gcaagtgatt tatatcgtga tttccgcgct tttgctcatc 840
tgctcgccga tcgccctttt gtattcgatt gaaaaaaatg accggatgct gctcatcacg 900
cttccgatca agcgaaaaac gatgttttgg gcgaaatatc gcttttattc aggcctattg 960
gcaggcggat ttctccttgt cgtgatgatt gtgggtttca 1000
<210> 512
<211> 239
<212> PRT
<213> 枯草芽孢杆菌
<400> 512
Met Ser Ile Leu Asp Ile His Asp Val Ser Val Trp Tyr Glu Arg Asp
1 5 10 15
Asn Val Ile Leu Glu Gln Val Asp Leu His Leu Glu Lys Gly Ala Val
20 25 30
Tyr Gly Leu Leu Gly Val Asn Gly Ala Gly Lys Thr Thr Leu Ile Asn
35 40 45
Thr Leu Thr Gly Val Asn Arg Asn Phe Ser Gly Arg Phe Thr Leu Cys
50 55 60
Gly Ile Glu Ala Glu Ala Gly Met Pro Gln Lys Thr Ser Asp Gln Leu
65 70 75 80
Lys Thr His Arg Tyr Phe Ala Ala Asp Tyr Pro Leu Leu Phe Thr Glu
85 90 95
Ile Thr Ala Lys Asp Tyr Val Ser Phe Val His Ser Leu Tyr Gln Lys
100 105 110
Asp Phe Ser Glu Gln Gln Phe Ala Ser Leu Ala Glu Ala Phe His Phe
115 120 125
Ser Lys Tyr Ile Asn Arg Arg Ile Ser Glu Leu Ser Leu Gly Asn Arg
130 135 140
Gln Lys Val Val Leu Met Thr Gly Leu Leu Leu Arg Ala Pro Leu Phe
145 150 155 160
Ile Leu Asp Glu Pro Leu Val Gly Leu Asp Val Glu Ser Ile Glu Val
165 170 175
Phe Tyr Gln Lys Met Arg Glu Tyr Cys Glu Ala Gly Gly Thr Ile Leu
180 185 190
Phe Ser Ser His Leu Leu Asp Val Val Gln Arg Phe Cys Asp Tyr Ala
195 200 205
Ala Ile Leu His Asn Lys Gln Ile Gln Lys Val Ile Pro Ile Gly Glu
210 215 220
Glu Thr Asp Leu Arg Arg Glu Phe Phe Glu Val Ile Gly His Glu
225 230 235
<210> 513
<211> 716
<212> DNA
<213> 枯草芽孢杆菌
<400> 513
gcattttgga tatacacgat gtatccgttt ggtatgaacg ggacaacgtc atcttagagc 60
acgtggactt acacttagaa aaaggcgccg tttacggatt gcttggggta aacggtgccg 120
gcaaaacaac actgatcaat acgctgacag gagtgaaccg caattacagc gggggcttta 180
cgctgtgcgg cattgaagct gaggccggca tgccgcagaa aacatcagat caactgaaga 240
ttcaccgtta cttcgccgct gattatccgc tgctgtttac agaaattacg gcgaaggact 300
atgtgtcttt cgtccattcg ctttatcaaa aggatttttc agagcgacag tttgccagtt 360
tggctgaggc ctttcatttt tcaaaataca tcaacaggag aatctcggag ctgtccttgg 420
ggaacaggca aaaggttgtg ttgatgacag gattattgct gcgggctccc ctgtttattt 480
tggatgagcc gctcgtcggt ttggatgtgg aatcaataga ggtcttttat cagaaaatgc 540
gggagtactg tgaggaaggc ggaaccattt tgttttcttc ccatctgctc gatgtcgtgc 600
agagattttg tgattttgcg gccattctgc acaacaaaca gatccaaaag gtcattccga 660
ttggggagga gaccgatctg cggcgggaat tttttgaggt tatcggccat gaataa 716
<210> 514
<211> 53
<212> PRT
<213> 枯草芽孢杆菌
<400> 514
Met Ser Pro Ala Gln Arg Arg Ile Leu Leu Tyr Ile Leu Ser Phe Ile
1 5 10 15
Phe Val Ile Gly Ala Val Val Tyr Phe Val Lys Ser Asp Tyr Leu Phe
20 25 30
Thr Leu Ile Phe Ile Ala Ile Ala Ile Leu Phe Gly Met Arg Ala Arg
35 40 45
Lys Ala Asp Ser Arg
50
<210> 515
<211> 162
<212> DNA
<213> 枯草芽孢杆菌
<400> 515
ttgtcaccag cacaaagaag aattttactg tatatccttt catttatctt tgtcatcggc 60
gcagtcgtct attttgtcaa aagcgattat ctgtttacgc tgattttcat tgccattgcc 120
attctgttcg ggatgcgcgc gcggaaggct gactcgcgat ga 162
<210> 516
<211> 87
<212> PRT
<213> 大肠杆菌
<400> 516
Met Glu Leu Lys Asn Ser Ile Ser Asp Tyr Thr Glu Ala Glu Phe Val
1 5 10 15
Gln Leu Leu Lys Glu Ile Glu Lys Glu Asn Val Ala Ala Thr Asp Asp
20 25 30
Val Leu Asp Val Leu Leu Glu His Phe Val Lys Ile Thr Glu His Pro
35 40 45
Asp Gly Thr Asp Leu Ile Tyr Tyr Pro Ser Asp Asn Arg Asp Asp Ser
50 55 60
Pro Glu Gly Ile Val Lys Glu Ile Lys Glu Trp Arg Ala Ala Asn Gly
65 70 75 80
Lys Pro Gly Phe Lys Gln Gly
85
<210> 517
<211> 264
<212> DNA
<213> 大肠杆菌
<400> 517
atggaactga aaaatagtat tagtgattac acagaggctg agtttgttca acttcttaag 60
gaaattgaaa aagagaatgt tgctgcaact gatgatgtgt tagatgtgtt actcgaacac 120
tttgtaaaaa ttactgagca tccagatgga acggatctga tttattatcc tagtgataat 180
agagacgata gccccgaagg gattgtcaag gaaattaaag aatggcgagc tgctaacggt 240
aagccaggat ttaaacaggg ctga 264
<210> 518
<211> 87
<212> PRT
<213> 绿脓假单胞菌
<400> 518
Met Lys Ser Lys Ile Ser Glu Tyr Thr Glu Lys Glu Phe Leu Glu Phe
1 5 10 15
Val Glu Asp Ile Tyr Thr Asn Asn Lys Lys Lys Phe Pro Thr Glu Glu
20 25 30
Ser His Ile Gln Ala Val Leu Glu Phe Lys Lys Leu Thr Glu His Pro
35 40 45
Ser Gly Ser Asp Leu Leu Tyr Tyr Pro Asn Glu Asn Arg Glu Asp Ser
50 55 60
Pro Ala Gly Val Val Lys Glu Val Lys Glu Trp Arg Ala Ser Lys Gly
65 70 75 80
Leu Pro Gly Phe Lys Ala Gly
85
<210> 519
<211> 264
<212> DNA
<213> 绿脓假单胞菌
<400> 519
atgaagtcca agatttccga atatacggaa aaagagtttc ttgagtttgt tgaagacata 60
tacacaaaca ataagaaaaa gttccctacc gaggagtctc atattcaagc cgtgcttgaa 120
tttaaaaaac taacggaaca cccaagcggc tcagaccttc tttactaccc caacgaaaat 180
agagaagata gcccagctgg agttgtaaag gaagttaaag aatggcgtgc ttccaagggg 240
cttcctggct ttaaggccgg ttag 264
<210> 520
<211> 87
<212> PRT
<213> 绿脓假单胞菌
<400> 520
Met Lys Ser Lys Ile Ser Glu Tyr Thr Glu Lys Glu Phe Leu Glu Phe
1 5 10 15
Val Lys Asp Ile Tyr Thr Asn Asn Lys Lys Lys Phe Pro Thr Glu Glu
20 25 30
Ser His Ile Gln Ala Val Leu Glu Phe Lys Lys Leu Thr Glu His Pro
35 40 45
Ser Gly Ser Asp Leu Leu Tyr Tyr Pro Asn Glu Asn Arg Glu Asp Ser
50 55 60
Pro Ala Gly Val Val Lys Glu Val Lys Glu Trp Arg Ala Ser Lys Gly
65 70 75 80
Leu Pro Gly Phe Lys Ala Gly
85
<210> 521
<211> 264
<212> DNA
<213> 绿脓假单胞菌
<400> 521
atgaagtcca agatttccga atatacggaa aaagagtttc ttgagtttgt taaagacata 60
tacacaaaca ataagaaaaa gttccctacc gaggagtctc atattcaagc cgtgcttgaa 120
tttaaaaaac taacggaaca cccaagcggc tcagaccttc tttactaccc caacgaaaat 180
agagaagata gcccagctgg agttgtaaag gaagttaaag aatggcgtgc ttccaagggg 240
cttcctggct ttaaggccgg ttag 264
<210> 522
<211> 95
<212> PRT
<213> 海氏肠球菌
<400> 522
Met Asp Phe Thr Lys Glu Glu Lys Leu Leu Asn Ala Ile Ser Lys Val
1 5 10 15
Tyr Asn Glu Ala Thr Ile Asp Asp Tyr Pro Asp Leu Lys Glu Lys Leu
20 25 30
Phe Leu Tyr Ser Lys Glu Ile Ser Glu Gly Lys Ser Val Gly Glu Val
35 40 45
Ser Met Lys Leu Ser Ser Phe Leu Gly Arg Tyr Ile Leu Lys His Lys
50 55 60
Phe Gly Leu Pro Lys Ser Leu Ile Glu Leu Gln Glu Ile Val Ser Lys
65 70 75 80
Glu Ser Gln Val Tyr Arg Gly Trp Ala Ser Ile Gly Ile Trp Ser
85 90 95
<210> 523
<211> 288
<212> DNA
<213> 海氏肠球菌
<400> 523
atggatttta ctaaagaaga aaaactttta aatgcaatta gtaaagtata caatgaagca 60
actatagatg actatcctga cttaaaagaa aagctctttc tttattctaa agaaatcagt 120
gagggaaaaa gtgttggtga agttagtatg aaattaagta gttttcttgg aagatatatt 180
ttaaaacata aatttggatt acctaaatct ttaatagaat tacaagaaat tgttagtaag 240
gaatctcaag tatatagagg atgggcttct attggtattt ggagttaa 288
<210> 524
<211> 113
<212> PRT
<213> 肠膜明串珠菌
<400> 524
Met Lys Lys Lys Tyr Arg Tyr Leu Glu Asp Ser Lys Asn Tyr Thr Ser
1 5 10 15
Thr Leu Tyr Ser Leu Leu Val Asp Asn Val Asp Lys Pro Gly Tyr Ser
20 25 30
Asp Ile Cys Asp Val Leu Leu Gln Val Ser Lys Lys Leu Asp Asn Thr
35 40 45
Gln Ser Val Glu Ala Leu Ile Asn Arg Leu Val Asn Tyr Ile Arg Ile
50 55 60
Thr Ala Ser Thr Tyr Lys Ile Ile Phe Ser Lys Lys Glu Glu Glu Leu
65 70 75 80
Ile Ile Lys Leu Gly Val Ile Gly Gln Lys Ala Gly Leu Asn Gly Gln
85 90 95
Tyr Met Ala Asp Phe Ser Asp Lys Ser Gln Phe Tyr Ser Val Phe Asp
100 105 110
Gln
<210> 525
<211> 342
<212> DNA
<213> 肠膜明串珠菌
<400> 525
ttgaaaaaaa agtatcggta tttagaagat agcaaaaatt acactagtac actctattct 60
ctgttagttg ataatgttga caaacctgga tactcagata tttgcgatgt tttgcttcaa 120
gtttctaaga agttggataa tactcaaagt gttgaagcgc taattaatcg attggttaat 180
tatattcgta ttactgcttc aacatacaaa attatttttt caaaaaaaga agaggaattg 240
attataaaac ttggtgttat tggacaaaaa gctggactta atggtcagta tatggctgat 300
ttttcagaca agtctcagtt ttacagcgtt ttcgatcagt aa 342
<210> 526
<211> 93
<212> PRT
<213> 大肠杆菌
<400> 526
Met Ser Phe Leu Asn Phe Ala Phe Ser Pro Val Phe Phe Ser Ile Met
1 5 10 15
Ala Cys Tyr Phe Ile Val Trp Arg Asn Lys Arg Asn Glu Phe Val Cys
20 25 30
Asn Arg Leu Leu Ser Ile Ile Ile Ile Ser Phe Leu Ile Cys Phe Ile
35 40 45
Tyr Pro Trp Leu Asn Tyr Lys Ile Glu Val Lys Tyr Tyr Ile Phe Glu
50 55 60
Gln Phe Tyr Leu Phe Cys Phe Leu Ser Ser Leu Val Ala Val Val Ile
65 70 75 80
Asn Leu Ile Val Tyr Phe Ile Leu Tyr Arg Arg Cys Ile
85 90
<210> 527
<211> 282
<212> DNA
<213> 大肠杆菌
<400> 527
atgagttttc ttaattttgc attttctcct gtattcttct ccattatggc gtgttatttc 60
attgtatgga gaaataaacg aaacgaattt gtctgcaata gattgctatc aattataata 120
atatcttttt tgatatgctt catatatcca tggctaaatt acaaaatcga agttaaatat 180
tatatatttg aacagtttta tcttttttgt tttttatcgt cactcgtggc tgttgtaata 240
aacctaattg tatactttat attatacagg agatgtatat ga 282
<210> 528
<211> 96
<212> PRT
<213> 大肠杆菌
<400> 528
Met His Leu Lys Tyr Tyr Leu His Asn Leu Pro Glu Ser Leu Ile Pro
1 5 10 15
Trp Ile Leu Ile Leu Ile Phe Asn Asp Asn Asp Asn Thr Pro Leu Leu
20 25 30
Phe Ile Phe Ile Ser Ser Ile His Val Leu Leu Tyr Pro Tyr Ser Lys
35 40 45
Leu Thr Ile Ser Arg Tyr Ile Lys Glu Asn Thr Lys Leu Lys Lys Glu
50 55 60
Pro Trp Tyr Leu Cys Lys Leu Ser Ala Leu Phe Tyr Leu Leu Met Ala
65 70 75 80
Ile Pro Val Gly Leu Pro Ser Phe Ile Tyr Tyr Thr Leu Lys Arg Asn
85 90 95
<210> 529
<211> 291
<212> DNA
<213> 大肠杆菌
<400> 529
atgcatttaa aatactacct acataattta cctgaatcac ttataccatg gattcttatt 60
ttaatattta acgacaatga taacactcct ttgttattta tatttatatc atcaatacat 120
gtattgctat atccatactc taaattaacc atatctagat atatcaaaga aaatacaaag 180
ttaaaaaaag aaccctggta cttatgcaag ttatctgcat tgttttattt attaatggca 240
atcccagtag gattgccaag tttcatatat tacactctaa agagaaatta a 291
<210> 530
<211> 344
<212> PRT
<213> 大肠杆菌
<400> 530
Met Met Ile Gln Ser His Pro Leu Leu Ala Ala Pro Leu Ala Val Gly
1 5 10 15
Asp Thr Ile Gly Phe Phe Ser Ser Ser Ala Pro Ala Thr Val Thr Ala
20 25 30
Lys Asn Arg Phe Phe Arg Gly Val Glu Phe Leu Gln Arg Lys Gly Phe
35 40 45
Lys Leu Val Ser Gly Lys Leu Thr Gly Lys Thr Asp Phe Tyr Arg Ser
50 55 60
Gly Thr Ile Lys Glu Arg Ala Gln Glu Phe Asn Glu Leu Val Tyr Asn
65 70 75 80
Pro Asp Ile Thr Cys Ile Met Ser Thr Ile Gly Gly Asp Asn Ser Asn
85 90 95
Ser Leu Leu Pro Phe Leu Asp Tyr Asp Ala Ile Ile Ala Asn Pro Lys
100 105 110
Ile Ile Ile Gly Tyr Ser Asp Thr Thr Ala Leu Leu Ala Gly Ile Tyr
115 120 125
Ala Lys Thr Gly Leu Ile Thr Phe Tyr Gly Pro Ala Leu Ile Pro Ser
130 135 140
Phe Gly Glu His Pro Pro Leu Val Asp Ile Thr Tyr Glu Ser Phe Ile
145 150 155 160
Lys Ile Leu Thr Arg Lys Gln Ser Gly Ile Tyr Thr Tyr Thr Leu Pro
165 170 175
Glu Lys Trp Ser Asp Glu Ser Ile Asn Trp Asn Glu Asn Lys Ile Leu
180 185 190
Arg Pro Lys Lys Leu Tyr Lys Asn Asn Cys Ala Phe Tyr Gly Ser Gly
195 200 205
Lys Val Glu Gly Arg Val Ile Gly Gly Asn Leu Asn Thr Leu Thr Gly
210 215 220
Ile Trp Gly Ser Glu Trp Met Pro Glu Ile Leu Asn Gly Asp Ile Leu
225 230 235 240
Phe Ile Glu Asp Ser Arg Lys Ser Ile Ala Thr Ile Glu Arg Leu Phe
245 250 255
Ser Met Leu Lys Leu Asn Arg Val Phe Asp Lys Val Ser Ala Ile Ile
260 265 270
Leu Gly Lys His Glu Leu Phe Asp Cys Ala Gly Ser Lys Arg Arg Pro
275 280 285
Tyr Glu Val Leu Thr Glu Val Leu Asp Gly Lys Gln Ile Pro Val Leu
290 295 300
Asp Gly Phe Asp Cys Ser His Thr His Pro Met Leu Thr Leu Pro Leu
305 310 315 320
Gly Val Lys Leu Ala Ile Asp Phe Asp Asn Lys Asn Ile Ser Ile Thr
325 330 335
Glu Gln Tyr Leu Ser Thr Glu Lys
340
<210> 531
<211> 1000
<212> DNA
<213> 大肠杆菌
<400> 531
atgatgatac aatctcatcc actactggcc gctcccctgg cagtaggaga tacaattggt 60
ttcttttcat catctgctcc ggcaacagtt actgcaaaaa atcgtttttt tcggggagtt 120
gagtttcttc agagaaaggg atttaagctg gtatcaggga agcttaccgg taaaacagat 180
ttttatcgtt caggtactat taaagaaaga gctcaagaat ttaatgagtt agtctacaat 240
cctgatatta cctgtataat gtcaacgatc ggtggagata acagtaattc actactaccg 300
tttctggact atgatgctat cattgcaaac cccaaaatta tcataggtta ctcagataca 360
actgctttat tagcaggaat atatgcaaaa acagggttaa taacattcta tggaccagct 420
cttattcctt cgtttggtga acatccacct cttgtggata taacatatga atcatttatt 480
aaaatactaa caagaaaaca atcaggaata tatacctaca cattacctga aaagtggagt 540
gatgagagca taaactggaa tgaaaacaag atattaaggc ctaagaagct atataaaaac 600
aactgtgcct tttatggttc cggaaaagtt gaggggcgtg taattggagg aaatctaaat 660
actttgacag gtatatgggg gagtgaatgg atgcctgaaa ttcttaatgg agatatattg 720
tttattgagg acagtcggaa aagcattgca acaattgaac gattattctc tatgctaaag 780
cttaatcgcg tgtttgataa agttagtgca ataatactcg ggaaacatga gctttttgat 840
tgtgcaggaa gtaaacgcag accatatgaa gtattaacag aggtattaga tgggaaacag 900
attcctgtac tggatggatt tgattgttca catacacatc caatgctaac tcttccactt 960
ggtgtaaaat tagctattga ctttgacaac aaaaatatat 1000
<210> 532
<211> 90
<212> PRT
<213> 清酒乳杆菌
<400> 532
Met Lys Ala Asp Tyr Lys Lys Ile Asn Ser Ile Leu Thr Tyr Thr Ser
1 5 10 15
Thr Ala Leu Lys Asn Pro Lys Ile Ile Lys Asp Lys Asp Leu Val Val
20 25 30
Leu Leu Thr Ile Ile Gln Glu Glu Ala Lys Gln Asn Arg Ile Phe Tyr
35 40 45
Asp Tyr Lys Arg Lys Phe Arg Pro Ala Val Thr Arg Phe Thr Ile Asp
50 55 60
Asn Asn Phe Glu Ile Pro Asp Cys Leu Val Lys Leu Leu Ser Ala Val
65 70 75 80
Glu Thr Pro Lys Ala Trp Ser Gly Phe Ser
85 90
<210> 533
<211> 268
<212> DNA
<213> 清酒乳杆菌
<400> 533
ggcagattat aaaaaaataa attcaatact aacttacaca tctactgctt taaaaaaccc 60
taaaattata aaagataaag atttagtagt ccttctaact attattcaag aagaagccaa 120
acaaaataga atcttttatg attataaaag aaaatttcgt ccagcggtta ctcgctttac 180
aattgataat aattttgaga ttcctgattg tttggttaaa ctactgtcag ctgttgaaac 240
acctaaggcg tggtctggat ttagttag 268
<210> 534
<211> 83
<212> PRT
<213> 大肠杆菌
<400> 534
Met Lys Leu Ser Pro Lys Ala Ala Ile Glu Val Cys Asn Glu Ala Ala
1 5 10 15
Lys Lys Gly Leu Trp Ile Leu Gly Ile Asp Gly Gly His Trp Leu Asn
20 25 30
Pro Gly Phe Arg Ile Asp Ser Ser Ala Ser Trp Thr Tyr Asp Met Pro
35 40 45
Glu Glu Tyr Lys Ser Lys Thr Pro Glu Asn Asn Arg Leu Ala Ile Glu
50 55 60
Asn Ile Lys Asp Asp Ile Glu Asn Gly Tyr Thr Ala Phe Ile Ile Thr
65 70 75 80
Leu Lys Met
<210> 535
<211> 251
<212> DNA
<213> 大肠杆菌
<400> 535
tgaagttatc accaaaagct gcaatagaag tttgtaatga agcagcgaaa aaaggcttat 60
ggattttggg cattgatggt gggcattggc tgaatcctgg attcaggata gatagttcag 120
catcatggac atatgatatg ccggaggaat acaaatcaaa aacccctgaa aataatagat 180
tggctattga aaatattaaa gatgatattg agaatggata cactgctttc attatcacgt 240
taaagatgta a 251
<210> 536
<211> 436
<212> PRT
<213> 枯草芽孢杆菌
<400> 536
Met Asn Asn Ile Phe Pro Ile Met Ser Leu Leu Phe Lys Gln Leu Tyr
1 5 10 15
Ser Arg Gln Gly Lys Lys Asp Ala Ile Arg Ile Ala Ala Gly Leu Val
20 25 30
Ile Leu Ala Val Phe Glu Ile Gly Leu Ile Arg Gln Ala Gly Ile Asp
35 40 45
Glu Ser Val Leu Gly Lys Thr Tyr Ile Ile Leu Ala Leu Leu Leu Met
50 55 60
Asn Thr Tyr Met Val Phe Leu Ser Val Thr Ser Gln Trp Lys Glu Ser
65 70 75 80
Tyr Met Lys Leu Ser Cys Leu Leu Pro Ile Ser Ser Arg Ser Phe Trp
85 90 95
Leu Ala Gln Ser Val Val Leu Phe Val Asp Thr Cys Leu Arg Arg Thr
100 105 110
Leu Phe Phe Phe Ile Leu Pro Leu Phe Leu Phe Gly Asn Gly Thr Leu
115 120 125
Ser Gly Ala Gln Thr Leu Phe Trp Leu Gly Arg Phe Ser Phe Phe Thr
130 135 140
Val Tyr Ser Ile Leu Phe Gly Val Met Leu Ser Asn His Phe Val Lys
145 150 155 160
Lys Lys Asn Ser Met Phe Leu Leu His Ala Ala Val Phe Ala Phe Val
165 170 175
Cys Leu Ser Ala Ala Phe Met Pro Ala Val Thr Ile Pro Leu Cys Ala
180 185 190
Val His Met Leu Trp Ala Val Ile Ile Asp Phe Pro Val Phe Leu Gln
195 200 205
Ala Pro Pro His Gln Ser Lys Met His Phe Phe Met Arg Arg Ser Glu
210 215 220
Phe Ser Phe Tyr Lys Arg Glu Trp Asn Arg Phe Ile Ser Ser Lys Ala
225 230 235 240
Met Leu Leu Asn Tyr Val Val Met Ala Ala Phe Ser Gly Phe Phe Ser
245 250 255
Phe Gln Met Met Asn Thr Gly Ile Phe Asn Gln Gln Val Ile Tyr Ile
260 265 270
Val Ile Ser Ala Leu Leu Leu Ile Cys Ser Pro Ile Ala Leu Leu Tyr
275 280 285
Ser Ile Glu Lys Asn Asp Arg Met Leu Leu Ile Thr Leu Pro Ile Lys
290 295 300
Arg Arg Thr Met Phe Trp Ala Lys Tyr Arg Phe Tyr Ser Gly Leu Leu
305 310 315 320
Ala Gly Gly Phe Leu Leu Val Ala Ile Ile Val Gly Phe Ile Ser Gly
325 330 335
Arg Pro Ile Ser Ala Leu Thr Phe Val Gln Cys Met Glu Leu Leu Leu
340 345 350
Ala Gly Ala Phe Ile Arg Leu Thr Ala Asp Glu Lys Arg Pro Ser Phe
355 360 365
Gly Trp Gln Thr Glu Gln Gln Leu Trp Ser Gly Phe Ser Lys Tyr Arg
370 375 380
Ser Tyr Leu Phe Cys Leu Pro Leu Phe Leu Ala Thr Leu Ala Gly Thr
385 390 395 400
Ala Val Ser Leu Ala Val Ile Pro Ile Ala Ala Leu Ile Ile Val Tyr
405 410 415
Tyr Leu Gln Lys Gln Asp Gly Gly Phe Phe Asp Thr Ser Lys Arg Glu
420 425 430
Arg Ile Gly Ser
435
<210> 537
<211> 1000
<212> DNA
<213> 枯草芽孢杆菌
<400> 537
ttggggagga gaccgatctg cggcgggaat tttttgaggt tatcggccat gaataacata 60
ttccccatca tgtcgttgct gttcaaacag ctgtacagcc ggcaagggaa aaaggacgct 120
atccgcattg ctgcagggct tgtgattctc gccgtgtttg aaatcgggct gatccgacaa 180
gccggcattg acgaatcggt gttgggaaaa acgtatatca tattggcgct tctcttaatg 240
aacacgtata tggtgtttct ttccgtgaca tcacaatgga aggaatctta tatgaagctg 300
agctgtctgc tgccgatttc atcacggagc ttttggctcg cccagagtgt cgttctgttt 360
gtcgatacct gtttgagaag aacgttattc ttttttattt taccgctgtt cttatttgga 420
aacggaacgc tgtcaggggc gcaaacattg ttttggcttg gcagattttc gttttttacc 480
gtttactcga ttctattcgg agttatgcta agcaaccatt tcgtcaaaaa gaagaactcg 540
atgtttctgc tgcatgcggc ggtattcgcc tttgtatgcc tcagtgccgc ttttatgccg 600
gccgtcacga tcccgctatg cgcggttcac atgctatggg cggtgatcat tgactttccg 660
gtctttctgc aggcgcctcc gcatcagagc aagatgcatt tttttatgcg gcgatctgaa 720
ttttcgtttt acaaaagaga atggaaccga tttatttctt ctaaagcgat gctgttaaat 780
tacgtggtga tggcggcgtt cagcggattc ttttcgttcc agatgatgaa cactggcatc 840
ttcaatcagc aagtgattta tattgtgatt tccgctctat tgctgatttg ctcgccgatc 900
gcccttttgt actctattga aaaaaacgat cgcatgctgc tcatcacgct tccaattaaa 960
agaagaacga tgttttgggc gaaatatcgc ttttattcag 1000
<210> 538
<211> 580
<212> PRT
<213> 大肠杆菌
<400> 538
Met Glu Arg Lys Gln Lys Asn Ser Leu Phe Asn Tyr Ile Tyr Ser Leu
1 5 10 15
Met Asp Val Arg Gly Lys Phe Leu Phe Phe Ser Met Leu Phe Ile Thr
20 25 30
Ser Leu Ser Ser Ile Ile Ile Ser Ile Ser Pro Leu Ile Leu Ala Lys
35 40 45
Ile Thr Asp Leu Leu Ser Gly Ser Leu Ser Asn Phe Ser Tyr Glu Tyr
50 55 60
Leu Val Leu Leu Ala Cys Leu Tyr Met Phe Cys Val Ile Ser Asn Lys
65 70 75 80
Ala Ser Val Phe Leu Phe Met Ile Leu Gln Ser Ser Leu Arg Ile Asn
85 90 95
Met Gln Lys Lys Met Ser Leu Lys Tyr Leu Arg Glu Leu Tyr Asn Glu
100 105 110
Asn Ile Thr Asn Leu Ser Lys Asn Asn Ala Gly Tyr Thr Thr Gln Ser
115 120 125
Leu Asn Gln Ala Ser Asn Asp Ile Tyr Ile Leu Val Arg Asn Val Ser
130 135 140
Gln Asn Ile Leu Ser Pro Val Ile Gln Leu Ile Ser Thr Ile Val Val
145 150 155 160
Val Leu Ser Thr Lys Asp Trp Phe Ser Ala Gly Val Phe Phe Leu Tyr
165 170 175
Ile Leu Val Phe Val Ile Phe Asn Thr Arg Leu Thr Gly Ser Leu Ala
180 185 190
Ser Leu Arg Lys His Ser Met Asp Ile Thr Leu Asn Ser Tyr Ser Leu
195 200 205
Leu Ser Asp Thr Val Asp Asn Met Ile Ala Ala Lys Lys Asn Asn Ala
210 215 220
Leu Arg Leu Ile Ser Glu Arg Tyr Glu Asp Ala Leu Thr Gln Glu Asn
225 230 235 240
Asn Ala Gln Lys Lys Tyr Trp Leu Leu Ser Ser Lys Val Leu Leu Leu
245 250 255
Asn Ser Leu Leu Ala Val Ile Leu Phe Gly Ser Val Phe Ile Tyr Asn
260 265 270
Ile Leu Gly Val Leu Asn Gly Val Val Ser Ile Gly His Phe Ile Met
275 280 285
Ile Thr Ser Tyr Ile Ile Leu Leu Ser Thr Pro Val Glu Asn Ile Gly
290 295 300
Ala Leu Leu Ser Glu Ile Arg Gln Ser Met Ser Ser Leu Ala Gly Phe
305 310 315 320
Ile Gln Arg His Ala Glu Asn Lys Ala Thr Ser Pro Ser Ile Pro Phe
325 330 335
Leu Asn Met Glu Arg Lys Leu Asn Leu Ser Ile Arg Glu Leu Ser Phe
340 345 350
Ser Tyr Ser Asp Asp Lys Lys Ile Leu Asn Ser Val Ser Leu Asp Leu
355 360 365
Phe Thr Gly Lys Met Tyr Ser Leu Thr Gly Pro Ser Gly Ser Gly Lys
370 375 380
Ser Thr Leu Val Lys Ile Ile Ser Gly Tyr Tyr Lys Asn Tyr Phe Gly
385 390 395 400
Asp Ile Tyr Leu Asn Asp Ile Ser Leu Arg Asn Ile Ser Asp Glu Asp
405 410 415
Leu Asn Asp Ala Ile Tyr Tyr Leu Thr Gln Asp Asp Tyr Ile Phe Met
420 425 430
Asp Thr Leu Arg Phe Asn Leu Arg Leu Ala Asn Tyr Asp Ala Ser Glu
435 440 445
Asn Glu Ile Phe Lys Val Leu Lys Leu Ala Asn Leu Ser Val Val Asn
450 455 460
Asn Glu Pro Val Ser Leu Asp Thr His Leu Ile Asn Arg Gly Asn Asn
465 470 475 480
Tyr Ser Gly Gly Gln Lys Gln Arg Ile Ser Leu Ala Arg Leu Phe Leu
485 490 495
Arg Lys Pro Ala Ile Ile Ile Ile Asp Glu Ala Thr Ser Ala Leu Asp
500 505 510
Tyr Ile Asn Glu Ser Glu Ile Leu Ser Ser Ile Arg Thr His Phe Pro
515 520 525
Asp Ala Leu Ile Ile Asn Ile Ser His Arg Ile Asn Leu Leu Glu Cys
530 535 540
Ser Asp Cys Val Tyr Val Leu Asn Glu Gly Asn Ile Val Ala Ser Gly
545 550 555 560
His Phe Arg Asp Leu Met Val Ser Asn Glu Tyr Ile Ser Gly Leu Ala
565 570 575
Ser Val Thr Glu
580
<210> 539
<211> 1000
<212> DNA
<213> 大肠杆菌
<400> 539
atggaaagaa aacagaaaaa ctcattattt aattatattt attcattaat ggatgtaaga 60
ggtaaatttt tattcttttc catgttattc attacatcat tatcatcgat aatcatatct 120
atttcaccat tgattcttgc aaagattaca gatttactgt ctggctcatt gtcaaatttt 180
agttatgaat atctggtttt acttgcctgt ttatacatgt tttgcgttat atctaataaa 240
gcaagtgttt ttttatttat gatactgcaa agtagtctac gtattaacat gcagaaaaaa 300
atgtcgctaa agtatttgag agaattgtat aacgaaaata taactaactt gagtaaaaat 360
aatgctggat atacaacgca aagtcttaac caggcttcaa atgacattta tattcttgtg 420
agaaatgttt cccagaatat cctgtcacct gttatacaac ttatttccac tattgttgtt 480
gttttatcta cgaaggactg gttttctgcc ggtgtgtttt ttctctatat tctggtattt 540
gtaattttta ataccagact gactggcagt ttagcgtctc tcagaaaaca cagcatggat 600
atcactctta actcttatag tctgttatct gatactgttg ataacatgat agcagctaaa 660
aagaataatg cattaagact tatttctgaa cgttatgaag atgctctcac tcaggaaaac 720
aatgctcaga aaaaatactg gttactcagt tctaaagttc ttttattgaa ctctttactt 780
gctgtaatat tatttggttc tgtattcata tataatattt taggtgtgct gaatggtgta 840
gttagtatcg gccacttcat tatgattaca tcatatatca ttcttctttc aacgccagtg 900
gaaaatatag gggcattgct aagtgagatc aggcagtcaa tgtctagcct ggcaggtttt 960
attcaacgtc atgccgagaa taaagccaca tctccttcaa 1000
<210> 540
<211> 95
<212> PRT
<213> 肺炎克雷伯菌
<400> 540
Met Thr Leu Leu Ser Phe Gly Phe Ser Pro Val Phe Phe Ser Val Met
1 5 10 15
Ala Phe Cys Ile Ile Ser Arg Ser Lys Phe Tyr Pro Gln Arg Thr Arg
20 25 30
Asn Lys Val Ile Val Leu Ile Leu Leu Thr Phe Phe Ile Cys Phe Leu
35 40 45
Tyr Pro Leu Thr Lys Val Tyr Leu Val Gly Ser Tyr Gly Ile Phe Asp
50 55 60
Lys Phe Tyr Leu Phe Cys Phe Ile Ser Thr Leu Ile Ala Ile Ala Ile
65 70 75 80
Asn Val Val Ile Leu Thr Ile Asn Gly Ala Lys Asn Glu Arg Asn
85 90 95
<210> 541
<211> 288
<212> DNA
<213> 肺炎克雷伯菌
<400> 541
atgacattac tttcatttgg attttctcct gttttctttt cagtcatggc gttctgtatc 60
atttcacgta gtaaattcta tccgcagaga acgcgaaaca aagttattgt tctgatttta 120
ctaacttttt ttatttgttt tttatatcca ttaacaaaag tgtatctggt gggaagttac 180
ggtatatttg acaaattcta cctcttttgc tttatttcta cgttaattgc aatagcaatt 240
aacgtagtga tacttacaat aaatggagct aagaatgaga gaaattag 288
<210> 542
<211> 13
<212> RNA
<213> 人工序列
<220>
<223> Kozak序列
<220>
<221> misc_feature
<222> (7)..(7)
<223> n = a或g
<400> 542
gccgccrcca ugg 13
<210> 543
<211> 6
<212> RNA
<213> 人工序列
<220>
<223> Shine-Delgarno序列
<400> 543
ggaggu 6
<210> 544
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 前导启动子
<400> 544
gaaaaccttg tcaatgaaga gcgatctatg 30
<210> 545
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> FecA启动子
<400> 545
ttctcgttcg actcatagct gaacacaaca 30
<210> 546
<211> 16
<212> DNA
<213> 人工序列
<220>
<223> Cu-敏感启动子
<400> 546
atgacaaaat tgtcat 16
<210> 547
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> Fe启动子
<400> 547
accaatgctg ggaacggcca gggcacctaa 30
<210> 548
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> Fe和UV启动子
<400> 548
ctgaaagcgc ataccgctat ggagggggtt 30
<210> 549
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> PrFe (PI + PII rus操纵子)
<400> 549
tagatatgcc tgaaagcgca taccgctatg 30
<210> 550
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> Lux盒式右启动子
<400> 550
tgttatagtc gaatacctct ggcggtgata 30
<210> 551
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> P(Las) TetO
<400> 551
ttttggtaca ctccctatca gtgatagaga 30
<210> 552
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> P(Las) CIO
<400> 552
ctttttggta cactacctct ggcggtgata 30
<210> 553
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> P(Rhl)
<400> 553
tacgcaagaa aatggtttgt tatagtcgaa 30
<210> 554
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 双启动子(LuxR/HSL, 正 / cI, 负)
<400> 554
cgtgcgtgtt gataacaccg tgcgtgttga 30
<210> 555
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 金黄色葡萄球菌agr操纵子中的P2启动子
<400> 555
agattgtact aaatcgtata atgacagtga 30
<210> 556
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> plux-cI杂交启动子
<400> 556
gtgttgatgc ttttatcacc gccagtggta 30
<210> 557
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> plux-lac杂交启动子
<400> 557
agtgtgtgga attgtgagcg gataacaatt 30
<210> 558
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> CinR、CinL和葡萄糖控制的启动子
<400> 558
acatcttaaa agttttagta tcatattcgt 30
<210> 559
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> CI可抑制的RhIR启动子
<400> 559
tacgcaagaa aatggtttgt tatagtcgaa 30
<210> 560
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 反向Lux启动子
<400> 560
tcttgcgtaa acctgtacga tcctacaggt 30
<210> 561
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> rhlI启动子
<400> 561
atcctccttt agtcttcccc ctcatgtgtg 30
<210> 562
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> lasI启动子
<400> 562
taaaattatg aaatttgcat aaattcttca 30
<210> 563
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> LuxR+3OC6HSL非依赖性R0065
<400> 563
gtgttgacta ttttacctct ggcggtgata 30
<210> 564
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> LasR/LasI诱导型& RHLR/RHLI可抑制的启动子
<400> 564
gaaatctggc agtttttggt acacgaaagc 30
<210> 565
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> pLux/cI杂交启动子
<400> 565
acaccgtgcg tgttgatata gtcgaataaa 30
<210> 566
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> pLas启动子
<400> 566
aaaattatga aatttgtata aattcttcag 30
<210> 567
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> pLas/cI杂交启动子
<400> 567
ggttcttttt ggtacctctg gcggtgataa 30
<210> 568
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> pLas/Lux杂交启动子
<400> 568
tgtaggatcg tacaggtata aattcttcag 30
<210> 569
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> pLux
<400> 569
caagaaaatg gtttgttata gtcgaataaa 30
<210> 570
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> pLux/Las杂交启动子
<400> 570
ctatctcatt tgctagtata gtcgaataaa 30
<210> 571
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 杂交启动子: HSL-LuxR激活的, P22 C2抑制的
<400> 571
tagtttataa tttaagtgtt ctttaatttc 30
<210> 572
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> PAI+LasR -> LuxI (AI)
<400> 572
caccttcggg tgggcctttc tgcgtttata 30
<210> 573
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> PAI+LasR -> LasI & AI+LuxR --| LasI
<400> 573
aataactctg atagtgctag tgtagatctc 30
<210> 574
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> PAI+LasR -> LasI+GFP & AI+LuxR --| LasI+GFP
<400> 574
caccttcggg tgggcctttc tgcgtttata 30
<210> 575
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> Complex QS -> LuxI & LasI环
<400> 575
caccttcggg tgggcctttc tgcgtttata 30
<210> 576
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 位置3突变的启动子lux pR-3 (luxR & HSL调控的)
<400> 576
caagaaaatg gtttgttata gtcgaataaa 30
<210> 577
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 位置5突变的启动子 lux pR-5 (luxR & HSL调控的)
<400> 577
caagaaaatg gtttgttata gtcgaataaa 30
<210> 578
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 位置3&5突变的启动子 lux pR-3/5 (luxR & HSL调控的)
<400> 578
caagaaaatg gtttgttata gtcgaataaa 30
<210> 579
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 启动子 (HSL-介导的luxR阻遏物)
<400> 579
ttgacacctg taggatcgta caggtataat 30
<210> 580
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 启动子 (luxR & HSL调控的 -- lux pR)
<400> 580
caagaaaatg gtttgttata gtcgaataaa 30
<210> 581
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 启动子 (luxR & HSL调控的 -- lux pL)
<400> 581
cacgcaaaac ttgcgacaaa caataggtaa 30
<210> 582
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 启动子 (RhlR & C4-HSL调控的)
<400> 582
gttagctttc gaattggcta aaaagtgttc 30
<210> 583
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 启动子 (cinR和HSL调控的)
<400> 583
ccattctgct ttccacgaac ttgaaaacgc 30
<210> 584
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 启动子 (LasR & PAI调控的)
<400> 584
ggccgcgggt tctttttggt acacgaaagc 30
<210> 585
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 启动子, 标准(luxR和HSL调控的 -- lux pR)
<400> 585
aagaaaatgg tttgttgata ctcgaataaa 30
<210> 586
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> P(Bla)
<400> 586
gtttatacat aggcgagtac tctgttatgg 30
<210> 587
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> P(Cat)
<400> 587
agaggttcca actttcacca taatgaaaca 30
<210> 588
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> P(Kat)
<400> 588
taaacaacta acggacaatt ctacctaaca 30
<210> 589
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 用于建立引物家族成员的模板
<400> 589
acatcaagcc aaattaaaca ggattaacac 30
<210> 590
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 反向λ cI调控的启动子
<400> 590
gaggtaaaat agtcaacacg cacggtgtta 30
<210> 591
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 关键启动子吸收3
<400> 591
caggccggaa taactcccta taatgcgcca 30
<210> 592
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 592
ggctagctca gtcctaggta cagtgctagc 30
<210> 593
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 593
agctagctca gtcctaggta ttatgctagc 30
<210> 594
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 594
agctagctca gtcctaggta ctgtgctagc 30
<210> 595
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 595
agctagctca gtcctaggga ttatgctagc 30
<210> 596
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 596
agctagctca gtcctaggta ttgtgctagc 30
<210> 597
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 597
ggctagctca gtcctaggta ctatgctagc 30
<210> 598
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 598
ggctagctca gtcctaggta tagtgctagc 30
<210> 599
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 599
ggctagctca gccctaggta ttatgctagc 30
<210> 600
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 600
agctagctca gtcctaggta taatgctagc 30
<210> 601
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 601
agctagctca gtcctaggga ctgtgctagc 30
<210> 602
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 602
ggctagctca gtcctaggta caatgctagc 30
<210> 603
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 603
ggctagctca gtcctaggta tagtgctagc 30
<210> 604
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 604
agctagctca gtcctaggga ttatgctagc 30
<210> 605
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 605
ggctagctca gtcctaggga ttatgctagc 30
<210> 606
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 606
ggctagctca gtcctaggta caatgctagc 30
<210> 607
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 607
agctagctca gcccttggta caatgctagc 30
<210> 608
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 608
agctagctca gtcctaggga ctatgctagc 30
<210> 609
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 609
agctagctca gtcctaggga ttgtgctagc 30
<210> 610
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 610
ggctagctca gtcctaggta ttgtgctagc 30
<210> 611
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子家族成员
<400> 611
agctagctca gtcctaggta taatgctagc 30
<210> 612
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> J23107的1bp突变体
<400> 612
ggctagctca gtcctaggta ttatgctagc 30
<210> 613
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> J23114的1bp突变体
<400> 613
ggctagctca gtcctaggta caatgctagc 30
<210> 614
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> pBAD反向
<400> 614
aaagtgtgac gccgtgcaaa taatcaatgt 30
<210> 615
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> NikR启动子, 抑制表达的转录因子的带状螺旋-螺旋家族的蛋白质
<400> 615
gacgaatact taaaatcgtc atacttattt 30
<210> 616
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> lacq_启动子
<400> 616
aaacctttcg cggtatggca tgatagcgcc 30
<210> 617
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> lacIQ -启动子序列
<400> 617
tgatagcgcc cggaagagag tcaattcagg 30
<210> 618
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 大肠杆菌CreABCD磷酸盐感应操纵子启动子
<400> 618
ttatttaccg tgacgaacta attgctcgtg 30
<210> 619
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> GlnRS启动子
<400> 619
catacgccgt tatacgttgt ttacgctttg 30
<210> 620
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> lacZ的组成型弱启动子
<400> 620
ttatgcttcc ggctcgtatg ttgtgtggac 30
<210> 621
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 突变的LacZ启动子
<400> 621
ttatgcttcc ggctcgtatg gtgtgtggac 30
<210> 622
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 在-10和 -35元件之间具有(TA)10的组成型启动子
<400> 622
atatatatat atatataatg gaagcgtttt 30
<210> 623
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 在-10和-35元件之间具有(TA)9的组成型启动子
<400> 623
atatatatat atatataatg gaagcgtttt 30
<210> 624
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 在-10和-35元件之间具有(C)10的组成型启动子
<400> 624
ccccgaaagc ttaagaatat aattgtaagc 30
<210> 625
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 在-10和-35元件之间具有(C)12的组成型启动子
<400> 625
ccccgaaagc ttaagaatat aattgtaagc 30
<210> 626
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 在-10至-35元件之间具有 13 bp的优化的(TA)重复组成型启动子
<400> 626
tgacaatata tatatatata taatgctagc 30
<210> 627
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 在-10至-35元件之间具有15 bp 的优化的(TA)重复组成型启动子
<400> 627
acaatatata tatatatata taatgctagc 30
<210> 628
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 在-10至-35元件之间具有 17 bp的优化的(TA)重复组成型启动子
<400> 628
aatatatata tatatatata taatgctagc 30
<210> 629
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 在-10至-35元件之间具有 19 bp的优化的(TA)重复组成型启动子
<400> 629
tatatatata tatatatata taatgctagc 30
<210> 630
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 在-10至-35元件之间 具有21 bp的优化的(TA)重复组成型启动子
<400> 630
tatatatata tatatatata taatgctagc 30
<210> 631
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 在-10至-35元件之间 具有17 bp的优化的(A)重复组成型启动子
<400> 631
aaaaaaaaaa aaaaaaaata taatgctagc 30
<210> 632
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 在-10至-35元件之间具有 18 bp的优化的(A)重复组成型启动子
<400> 632
aaaaaaaaaa aaaaaaaata taatgctagc 30
<210> 633
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> J23101:GFP
<400> 633
caccttcggg tgggcctttc tgcgtttata 30
<210> 634
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> J23119:IFP
<400> 634
caccttcggg tgggcctttc tgcgtttata 30
<210> 635
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> J23119:HO1
<400> 635
caccttcggg tgggcctttc tgcgtttata 30
<210> 636
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 红外信号报告子(J23119:IFP:J23119:HO1)
<400> 636
caccttcggg tgggcctttc tgcgtttata 30
<210> 637
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 双终止子 + 组成型启动子
<400> 637
ggctagctca gtcctaggta cagtgctagc 30
<210> 638
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 双终止子 + 组成型启动子 + 强RBS
<400> 638
tgctagctac tagagattaa agaggagaaa 30
<210> 639
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> IPTG诱导型Lac启动子盒
<400> 639
ttgtgagcgg ataacaagat actgagcaca 30
<210> 640
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> IPTG诱导型Lac启动子盒
<400> 640
ttgtgagcgg ataacaagat actgagcaca 30
<210> 641
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> IPTG诱导型Lac启动子盒
<400> 641
ttgtgagcgg ataacaagat actgagcaca 30
<210> 642
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> M13K07基因I启动子
<400> 642
cctgttttta tgttattctc tctgtaaagg 30
<210> 643
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> M13K07基因II启动子
<400> 643
aaatatttgc ttatacaatc ttcctgtttt 30
<210> 644
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> M13K07基因III启动子
<400> 644
gctgataaac cgatacaatt aaaggctcct 30
<210> 645
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> M13K07基因IV启动子
<400> 645
ctcttctcag cgtcttaatc taagctatcg 30
<210> 646
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> M13K07基因V启动子
<400> 646
atgagccagt tcttaaaatc gcataaggta 30
<210> 647
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> M13K07基因VI启动子
<400> 647
ctattgattg tgacaaaata aacttattcc 30
<210> 648
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> M13K07基因VIII启动子
<400> 648
gtttcgcgct tggtataatc gctgggggtc 30
<210> 649
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> M13110
<400> 649
ctttgcttct gactataata gtcagggtaa 30
<210> 650
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> g3的修饰启动子序列.
<400> 650
aaaccgatac aattaaaggc tcctgctagc 30
<210> 651
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子 I
<400> 651
caccacactg atagtgctag tgtagatcac 30
<210> 652
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 组成型启动子 II
<400> 652
gccggaataa ctccctataa tgcgccacca 30
<210> 653
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> --指定部分表--
<400> 653
ttgacaagct tttcctcagc tccgtaaact 30
<210> 654
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 全长固定相osmY启动子
<400> 654
ggtttcaaaa ttgtgatcta tatttaacaa 30
<210> 655
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 最小固定相osmY启动子
<400> 655
ggtttcaaaa ttgtgatcta tatttaacaa 30
<210> 656
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> htpG热休克启动子
<400> 656
tctattccaa taaagaaatc ttcctgcgtg 30
<210> 657
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 启动子 veg，用于枯草芽孢杆菌的组成型启动子
<400> 657
aaaaatgggc tcgtgttgta caataaatgt 30
<210> 658
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 启动子 43，用于枯草芽孢杆菌的组成型启动子
<400> 658
aaaaaaagcg cgcgattatg taaaatataa 30
<210> 659
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 用于枯草芽孢杆菌的强组成型启动子
<400> 659
aattgcagta ggcatgacaa aatggactca 30
<210> 660
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> PliaG
<400> 660
caagcttttc ctttataata gaatgaatga 30
<210> 661
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> PlepA
<400> 661
tctaagctag tgtattttgc gtttaatagt 30
<210> 662
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> Pveg
<400> 662
aatgggctcg tgttgtacaa taaatgtagt 30
<210> 663
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 用于枯草芽孢杆菌的启动子 ctc
<400> 663
atccttatcg ttatgggtat tgtttgtaat 30
<210> 664
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 用于枯草芽孢杆菌的启动子 gsiB
<400> 664
taaaagaatt gtgagcggga atacaacaac 30
<210> 665
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 启动子 43，用于枯草芽孢杆菌的组成型启动子
<400> 665
aaaaaaagcg cgcgattatg taaaatataa 30
<210> 666
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 来自沙门氏菌的Pspv2
<400> 666
tacaaaataa ttcccctgca aacattatca 30
<210> 667
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 来自沙门氏菌的Pspv
<400> 667
tacaaaataa ttcccctgca aacattatcg 30
<210> 668
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> T7启动子 (来自T7噬菌体的强启动子)
<400> 668
agggaataca agctacttgt tctttttgca 30
<210> 669
<211> 23
<212> DNA
<213> 人工序列
<220>
<223> T7启动子
<400> 669
taatacgact cactataggg aga 23
<210> 670
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> T7启动子
<400> 670
gaatttaata cgactcacta tagggaga 28
<210> 671
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> T7共有 -10及其余
<400> 671
taatacgact cactatagg 19
<210> 672
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 重叠 T7启动子
<400> 672
gagtcgtatt aatacgactc actatagggg 30
<210> 673
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 更多重叠T7启动子
<400> 673
agtgagtcgt actacgactc actatagggg 30
<210> 674
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 减弱重叠T7启动子
<400> 674
gagtcgtatt aatacgactc tctatagggg 30
<210> 675
<211> 23
<212> DNA
<213> 人工序列
<220>
<223> T7 共有启动子序列
<400> 675
taatacgact cactataggg aga 23
<210> 676
<211> 23
<212> DNA
<213> 人工序列
<220>
<223> T7 RNAP启动子
<400> 676
ttatacgact cactataggg aga 23
<210> 677
<211> 23
<212> DNA
<213> 人工序列
<220>
<223> T7 RNAP启动子
<400> 677
gaatacgact cactataggg aga 23
<210> 678
<211> 23
<212> DNA
<213> 人工序列
<220>
<223> T7 RNAP启动子
<400> 678
taatacgtct cactataggg aga 23
<210> 679
<211> 23
<212> DNA
<213> 人工序列
<220>
<223> T7 RNAP启动子
<400> 679
tcatacgact cactataggg aga 23
<210> 680
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> T7 强启动子
<400> 680
taatacgact cactataggg agaccacaac 30
<210> 681
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> T7 弱结合和进行性
<400> 681
taattgaact cactaaaggg agaccacagc 30
<210> 682
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> T7弱结合启动子
<400> 682
cgaagtaata cgactcacta ttagggaaga 30
<210> 683
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> pCyc (中等)启动子
<400> 683
acaaacacaa atacacacac taaattaata 30
<210> 684
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> pAdh (强)启动子
<400> 684
ccaagcatac aatcaactat ctcatataca 30
<210> 685
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> pSte5 (弱)启动子
<400> 685
gatacaggat acagcggaaa caacttttaa 30
<210> 686
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 酵母 ADH1启动子
<400> 686
tttcaagcta taccaagcat acaatcaact 30
<210> 687
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> cyc100最小启动子
<400> 687
cctttgcagc ataaattact atacttctat 30
<210> 688
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> cyc70最小启动子
<400> 688
cctttgcagc ataaattact atacttctat 30
<210> 689
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> cyc43最小启动子
<400> 689
cctttgcagc ataaattact atacttctat 30
<210> 690
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> cyc28最小启动子
<400> 690
cctttgcagc ataaattact atacttctat 30
<210> 691
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> cyc16最小启动子
<400> 691
cctttgcagc ataaattact atacttctat 30
<210> 692
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> pPGK1
<400> 692
ttatctactt tttacaacaa atataaaaca 30
<210> 693
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> pCYC酵母启动子
<400> 693
acaaacacaa atacacacac taaattaata 30
<210> 694
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 酵母GPD (TDH3)启动子
<400> 694
gtttcgaata aacacacata aacaaacaaa 30
<210> 695
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 酵母中等长度ADH1启动子
<400> 695
ccaagcatac aatcaactat ctcatataca 30
<210> 696
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 酵母 CLB1 启动子区，G2/M细胞周期特异性
<400> 696
accatcaaag gaagctttaa tcttctcata 30
<210> 697
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> CMV启动子
<400> 697
agaacccact gcttactggc ttatcgaaat 30
<210> 698
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> Ubc启动子
<400> 698
ggccgttttt ggcttttttg ttagacgaag 30
<210> 699
<211> 564
<212> DNA
<213> 大肠杆菌
<220>
<223> McbG基因
<400> 699
atggatataa tagaaaaaag aatcacaaaa cgacacctca gtgaaagtga attatcaggt 60
gtaaattatt acaattgtat atttgaacga atacagcttg acaactttaa ctttcgcgat 120
tgtgagttcg aaaaatgtcg cttcgtaaac tgctcaataa agaatttaaa acttaatttt 180
tttaaactta ttgactgtga attcaaagat tgtttattac aaggagtaaa tgcagcagac 240
attatgtttc cctgtacttt ttctcttgta aattgtgatt tgagatttgt tgattttatt 300
agtttacggt tacagaaatc tattttttta tcctgtcgct tccgtgattg tttatttgag 360
gaaacagacc ttcgtaaatc tgattttact ggttcggagt ttaataatac agagttcaga 420
cattctgatt tgtctcactg tgatttttca atgacagaag gtctcgatat aaatcctgag 480
ataaacagga tcttatctat aaagatacca caggaagcag gtttaaaaat acttaaacga 540
atgggagtgg ttgtaggggg atga 564
<210> 700
<211> 1770
<212> DNA
<213> 大肠杆菌
<220>
<223> MccE基因
<400> 700
atggcgcaaa aaataacacc atctaaaatt gttaaacatg cccgggagtt aattattaag 60
gggattgaat cgggcgataa ctcatttgtc atttttgatg tagatggagc tctggagcgt 120
ctggaacatt acaggtgtca acttaagagt tttttcccca acagcagtat cgcatattca 180
tataagtcaa ataatctggc acaatggtgc cagattatct caggtaaagg tttgtatgca 240
gaggtgtgct ctgttgatga aatgaacctc gctaaaagag acggttttaa ccggattgtt 300
tttgatggcc ctttaaaaaa aacgagtgaa ctattaaagg caatagagat tggggcatta 360
attgaagttg acaatattga tgaatgtaaa agacttaatg aactatgtaa attgcataag 420
ttgacatgta gaattcattt gagattatcg cattactatg atgataattt atcaagattt 480
ggcttatctg aaagtgaggc tattaattta ctggagatgt tgatcagtaa gtcagagtat 540
ctcattttag acgggttcca tttacatgtt ggctctaatc tacccaatgc ggaaaaaata 600
tgtaaagctg ttatacagta tcatgagttg attcttaggt atatgccaga tgatggcact 660
cttaatcttg gtagtgggat acctgcagat tctttctcag catccagtga taacccaact 720
ccatgtccgg aggtattttt ctcatcgata tatgatacaa taaaaaattg cttcggtact 780
gtatgtgata aatggaacta tatttttgaa cctgggagac atctggtcga agactttggc 840
tactttatag ggaaagttat tagtactaaa aacaggtatg gtgttaaagt tgctcaaact 900
aacattggta taaactggat accttcgatc agaaattggg accattcatt tacattgttt 960
cataatcata accatatttc agatgataaa tctgatgagt atattattgc tggattcaat 1020
tgctttgaat gtgattgctt gtttccttca gttattcttc ccagtaactt atctgattat 1080
ttattttctg ttcgagggtg tggtgcttat gatatgcaaa caggaaacca gtggacgcgt 1140
aatctttatg ctgtatatac cataacaaat gatgttgtta atatatcaag gattcacaga 1200
agagaacttg atttccggaa gtatgatgta tctcttacgc catcaggaat aaaagtaaat 1260
gatgagatta cattattgta tccagcgtta aaatacgctg aagagttata tttgctcatt 1320
aatcaaaaca aaataaattt cattaaaagc atggcatggc cagcgtttgt taataacatt 1380
tctgattctg tttctttcat tgagcaatca atgattgata accagaatga aaaagcatta 1440
atcctcttta ttaaatacaa aactaagata gctggcgttg tatcttttaa tattattgac 1500
catgccaata aaacagcata tattggctat tggttaggcg ctaactttca gggaaagggg 1560
attgtaacca atgctataaa taaactgata caggagtatg gggattcggg cgttataaaa 1620
agatttgtta taaaatgtat tgttgataat aaaaaaagta atgccacggc attgaggtgt 1680
ggcttcacct tagagggtgt tctgcaaaaa gcagaaatac tcaacggtgt atcatacgat 1740
caaaatattt attcgaaagt aattggttaa 1770
<210> 701
<211> 561
<212> DNA
<213> 人工序列
<220>
<223> MccE基因的C末端部分
<400> 701
atgttccgga agtatgatgt atctcttacg ccatcaggaa taaaagtaaa tgatgagatt 60
acattattgt atccagcgtt aaaatacgct gaagagttat atttgctcat taatcaaaac 120
aaaataaatt tcattaaaag catggcatgg ccagcgtttg ttaataacat ttctgattct 180
gtttctttca ttgagcaatc aatgattgat aaccagaatg aaaaagcatt aatcctcttt 240
attaaataca aaactaagat agctggcgtt gtatctttta atattattga ccatgccaat 300
aaaacagcat atattggcta ttggttaggc gctaactttc agggaaaggg gattgtaacc 360
aatgctataa ataaactgat acaggagtat ggggattcgg gcgttataaa aagatttgtt 420
ataaaatgta ttgttgataa taaaaaaagt aatgccacgg cattgaggtg tggcttcacc 480
ttagagggtg ttctgcaaaa agcagaaata ctcaacggtg tatcatacga tcaaaatatt 540
tattcgaaag taattggtta a 561
<210> 702
<211> 6904
<212> DNA
<213> 人工序列
<220>
<223> pSyn2-McbG
<400> 702
atggatataa tagaaaaaag aatcacaaaa cgacacctca gtgaaagtga attatcaggt 60
gtaaattatt acaattgtat atttgaacga atacagcttg acaactttaa ctttcgcgat 120
tgtgagttcg aaaaatgtcg cttcgtaaac tgctcaataa agaatttaaa acttaatttt 180
tttaaactta ttgactgtga attcaaagat tgtttattac aaggagtaaa tgcagcagac 240
attatgtttc cctgtacttt ttctcttgta aattgtgatt tgagatttgt tgattttatt 300
agtttacggt tacagaaatc tattttttta tcctgtcgct tccgtgattg tttatttgag 360
gaaacagacc ttcgtaaatc tgattttact ggttcggagt ttaataatac agagttcaga 420
cattctgatt tgtctcactg tgatttttca atgacagaag gtctcgatat aaatcctgag 480
ataaacagga tcttatctat aaagatacca caggaagcag gtttaaaaat acttaaacga 540
atgggagtgg ttgtaggggg atgacttctg ttttggcgga tgagagaaga ttttcagcct 600
gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag 660
tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga 720
tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa 780
aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc 840
tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt 900
ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga 960
cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat 1020
atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 1080
agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 1140
cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 1200
gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 1260
cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 1320
tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 1380
ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 1440
ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 1500
atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 1560
cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 1620
atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 1680
gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 1740
cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 1800
tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 1860
tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 1920
gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt 1980
gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 2040
atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 2100
atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 2160
aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 2220
aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 2280
ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 2340
ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 2400
tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 2460
ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 2520
acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 2580
gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 2640
cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 2700
aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 2760
atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 2820
gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 2880
gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgatta 2940
ccgacctttg agtgagcatc gatggcagct cgcgttgcat tttcggaagc gctcgttttc 3000
ggaaacgctt tgaagttcct attccgaagt tcctattctc tagctagaaa gtataggaac 3060
ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac 3120
gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca 3180
aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct 3240
ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt atctctagta 3300
ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc gaaaacaata 3360
cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga aaccgttcat 3420
aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa agtatgcatc 3480
gatgctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 3540
gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 3600
agcagatctg gcttttcaat tcatcatttt ttttttattc ttttttttga tttcggtttc 3660
tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca 3720
gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat 3780
tcttaaccca actgcacaga acaaaaaccg gaaacgaaga taaatcatgt cgaaagctac 3840
atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat 3900
gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact 3960
ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt 4020
gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa 4080
ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta 4140
ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt 4200
gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag 4260
aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg gagaatatac 4320
taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca 4380
aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg 4440
tttagatgac aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc 4500
tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa 4560
ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca 4620
gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaaaaaaaaa 4680
aattggaaag aaaaagctca gatctgctgg cacgacaggt ttcccgtggt gcactctcag 4740
tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac 4800
tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 4860
ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 4920
aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg 4980
tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc 5040
agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt 5100
ttggtcactt gatgcctccg tgtaaggggg aatttctgtt catgggggta atgataccga 5160
tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg 5220
aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc 5280
agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc 5340
atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac 5400
tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc 5460
agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc 5520
aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc 5580
aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg 5640
atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc 5700
caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt 5760
ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc 5820
tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat 5880
cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc 5940
ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc 6000
gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag 6060
caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa 6120
acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac 6180
cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac 6240
ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc 6300
ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa 6360
gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag 6420
gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa 6480
cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc 6540
gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc 6600
acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcc ggagcttatc 6660
gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct 6720
gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata 6780
atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctgttgaca 6840
attaatcatc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 6900
acag 6904
<210> 703
<211> 7807
<212> DNA
<213> 人工序列
<220>
<223> pSyn2-McbE/F
<400> 703
atggttacat taaaaatggc gataattttt ctgatggaac aaatcagaac ccctttcagc 60
ttaatatgga caattatgtc acctacagtg ttgttctttt tccttcattt caatgaaatt 120
gaacttcatt atggtgatac agcatggctc ggaaaacaaa tatcatggtt tgtaggctac 180
atttcttttt ctgttgttct gtttaattac tgtctgtatc tggtcggaag aagagaaagt 240
ggttttattg ctaccttcgt gcataatatg gatggtcgac tattatttat tcgttctcaa 300
ttaatagcct cacttatcat gtctattttg tatgtgtttt ttttcatttt agtcgtacta 360
acaggattcc aggcaagtcc agattatcaa atagtaatga ttattttaaa atcgatatac 420
ataaatgcat ttatgatggt ttcactcact ttcatggcat cgttcagagt cacattccaa 480
acagccagca ctatctattc tgtattaata actgtctgta tggtatcagg aattgtatca 540
ttaaaatata atgaggggat cgtttactgg ataaatcagg ttaatcctat cgccatctat 600
tctacaatac tccagtcaga ccaggagttg tctctgatga cgatattttt ctatagtata 660
atgttaatca taagtattat ttcagcacta accttcaaaa ctgagccagt ctggagttct 720
caatgaccat acctctttta gaaatcaact ctctgagctt ttcatataaa gtaaatttac 780
cccctgtttt caataatctg tcgcttaaga ttgagcaggg agagttgata ggtctgcttg 840
gagaaaatgg agccgggaag accactcttt ttaaccttat caggggcgga gtcagtaatt 900
atgaagggac cctcaagcga aatttctctg gtggagaatt agtaagtcta cctcaagtca 960
taaatctttc aggtacacta aggaatgagg aggttcttga tttgatttgc tgcttcaata 1020
agttaactaa aaaacaggca tggacagagc ttaatcataa atggaatgat aattttttca 1080
ttcgctatga taaaataaga cgtaaaagga cttacacggt aagttatgga gagaagcgtt 1140
ggcttatcat atctttgatg ctaacgcttt gcaaaaatgc ccgtttattt ttactggatg 1200
aaccaactgt aggcattgat attcagtaca gaatgatgtt atgggaactg ataaataaga 1260
tcaccgctga cgggaaaacg gtatttttct caactcatat ttttgatgaa ttaacaagag 1320
ataaaatccc cttctatatg ctttcaaaaa atagtataaa tcgttattct gatatgagtg 1380
attttattca atccaataat gaaacaactc cagaaaaggc atttattaaa gaagttatgg 1440
gaacaggaga ctgacacatg gatataactt ctgttttggc ggatgagaga agattttcag 1500
cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg 1560
cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc 1620
cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac 1680
gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 1740
tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag 1800
ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc 1860
tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta aatacattca 1920
aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 1980
aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 2040
cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 2100
ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 2160
cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 2220
ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 2280
gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 2340
gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 2400
acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 2460
cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 2520
acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 2580
ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 2640
ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 2700
gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 2760
atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 2820
ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 2880
attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 2940
ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 3000
aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 3060
aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 3120
ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg 3180
tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 3240
ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 3300
cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 3360
agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 3420
gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 3480
ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 3540
tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 3600
tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 3660
cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 3720
tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 3780
gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccga 3840
ttaccgacct ttgagtgagc atcgatggca gctcgcgttg cattttcgga agcgctcgtt 3900
ttcggaaacg ctttgaagtt cctattccga agttcctatt ctctagctag aaagtatagg 3960
aacttcagag cgcttttgaa aaccaaaagc gctctgaaga cgcactttca aaaaaccaaa 4020
aacgcaccgg actgtaacga gctactaaaa tattgcgaat accgcttcca caaacattgc 4080
tcaaaagtat ctctttgcta tatatctctg tgctatatcc ctatataacc tacccatcca 4140
cctttcgctc cttgaacttg catctaaact cgacctctac attttttatg tttatctcta 4200
gtattactct ttagacaaaa aaattgtagt aagaactatt catagagtga atcgaaaaca 4260
atacgaaaat gtaaacattt cctatacgta gtatatagag acaaaataga agaaaccgtt 4320
cataattttc tgaccaatga agaatcatca acgctatcac tttctgttca caaagtatgc 4380
atcgatgctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 4440
gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 4500
tgcagcagat ctggcttttc aattcatcat ttttttttta ttcttttttt tgatttcggt 4560
ttctttgaaa tttttttgat tcggtaatct ccgaacagaa ggaagaacga aggaaggagc 4620
acagacttag attggtatat atacgcatat gtagtgttga agaaacatga aattgcccag 4680
tattcttaac ccaactgcac agaacaaaaa ccggaaacga agataaatca tgtcgaaagc 4740
tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat 4800
catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt 4860
actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat 4920
cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta 4980
caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca 5040
gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt 5100
ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc 5160
tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata 5220
tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc 5280
tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt 5340
gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt 5400
ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc 5460
taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg 5520
ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaaaaaa 5580
aaaaattgga aagaaaaagc tcagatctgc tggcacgaca ggtttcccgt ggtgcactct 5640
cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt 5700
gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 5760
tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 5820
cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg 5880
tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc 5940
tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc 6000
tgtttggtca cttgatgcct ccgtgtaagg gggaatttct gttcatgggg gtaatgatac 6060
cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac 6120
tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca 6180
ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc 6240
agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca 6300
gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt 6360
tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa 6420
ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg 6480
gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga 6540
tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg 6600
ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga 6660
ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc 6720
gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac 6780
gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg 6840
tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat 6900
gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc 6960
cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc 7020
gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa 7080
taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat 7140
gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag 7200
tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct 7260
caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag 7320
taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc 7380
caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag 7440
cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac 7500
cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tccggagctt 7560
atcgactgca cggtgcacca atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg 7620
gctgtgcagg tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg 7680
ataatgtttt ttgcgccgac atcataacgg ttctggcaaa tattctgaaa tgagctgttg 7740
acaattaatc atcggctcgt ataatgtgtg gaattgtgag cggataacaa tttcacacag 7800
gaaacag 7807
<210> 704
<211> 4916
<212> DNA
<213> 人工序列
<220>
<223> pMcbG 1.0
<400> 704
catatgcgaa ttcgcggccg caaagcttgc ccgggatccc accaccacca ccaccactga 60
gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa 120
taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga 180
ggaactatat ccggattcag gagagcgttc accgacaaac aacagataaa acgaaaggcc 240
cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcatg 300
gggagacccc acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc 360
aggtgggacc accgcgctac tgccgccagg caaattctgt tttatcagac cgcttctgcg 420
ttctgattta atctgtatca ggctgaaaat cttctctcat ccgccaaaac agaagtcatc 480
cccctacaac cactcccatt cgtttaagta tttttaaacc tgcttcctgt ggtatcttta 540
tagataagat cctgtttatc tcaggattta tatcgagacc ttctgtcatt gaaaaatcac 600
agtgagacaa atcagaatgt ctgaactctg tattattaaa ctccgaacca gtaaaatcag 660
atttacgaag gtctgtttcc tcaaataaac aatcacggaa gcgacaggat aaaaaaatag 720
atttctgtaa ccgtaaacta ataaaatcaa caaatctcaa atcacaattt acaagagaaa 780
aagtacaggg aaacataatg tctgctgcat ttactccttg taataaacaa tctttgaact 840
cacagtcaat aagtttaaaa aaattaagtt ttaaattctt tattgagcag tttacgaagc 900
gacatttttc gaactcacaa tcgcgaaagt taaagttgtc aagctgtatt cgttcaaata 960
tacaattgta ataatttaca cctgataatt cactttcact gaggtgtcgt tttgtgattc 1020
ttttttctat tatatccatc tgtttcctgt gtgaaattgt tatccgctca caaaaggaat 1080
tatagtttca acagatcttg atgcaatggg ctcatttcag aatatttgcc agaaccgtta 1140
tgatgtcggc gcaaaaaaca ttatccagaa cgggagtgcg ccttgagcga cacgaattat 1200
gcagtgattt acgacctgca cagccatacc acagcttccg atggctgcct gacgccagaa 1260
gcattggtgc accgtgcagt cgataagccc ggatcgttcc actgagcgtc agaccccgta 1320
gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 1380
acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 1440
tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 1500
ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 1560
atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 1620
agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 1680
cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 1740
agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 1800
acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 1860
gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 1920
ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 1980
gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 2040
gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 2100
gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac 2160
cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata 2220
cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc 2280
tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt 2340
ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct 2400
gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc 2460
gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat 2520
gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt 2580
catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga 2640
tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatctgcag 2700
ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag 2760
gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg 2820
gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg 2880
ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg 2940
attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga 3000
gcacgatcat gcgcacccgt ggggccgcca tgccggcgat aatggcctgc ttctcgccga 3060
aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata 3120
ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga 3180
cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg 3240
cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca 3300
agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc 3360
gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 3420
aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt 3480
gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg 3540
tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata 3600
taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc 3660
agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc 3720
atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg 3780
gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta 3840
tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg 3900
atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg 3960
gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca 4020
ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc 4080
agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg 4140
cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc 4200
gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc 4260
aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc 4320
atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg 4380
cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt 4440
ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag 4500
gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat 4560
taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc 4620
atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc 4680
gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc 4740
gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc 4800
gtagaggatc gagatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga 4860
gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga gatata 4916
<210> 705
<211> 4892
<212> DNA
<213> 人工序列
<220>
<223> pMcbG 1.1
<400> 705
catatgcgaa ttcgcggccg caaagcttgc ccgggatccc accaccacca ccaccactga 60
gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa 120
taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga 180
ggaactatat ccggattcag gagagcgttc accgacaaac aacagataaa acgaaaggcc 240
cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcatg 300
gggagacccc acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc 360
aggtgggacc accgcgctac tgccgccagg caaattctgt tttatcagac cgcttctgcg 420
ttctgattta atctgtatca ggctgaaaat cttctctcat ccgccaaaac agaagtcatc 480
cccctacaac cactcccatt cgtttaagta tttttaaacc tgcttcctgt ggtatcttta 540
tagataagat cctgtttatc tcaggattta tatcgagacc ttctgtcatt gaaaaatcac 600
agtgagacaa atcagaatgt ctgaactctg tattattaaa ctccgaacca gtaaaatcag 660
atttacgaag gtctgtttcc tcaaataaac aatcacggaa gcgacaggat aaaaaaatag 720
atttctgtaa ccgtaaacta ataaaatcaa caaatctcaa atcacaattt acaagagaaa 780
aagtacaggg aaacataatg tctgctgcat ttactccttg taataaacaa tctttgaact 840
cacagtcaat aagtttaaaa aaattaagtt ttaaattctt tattgagcag tttacgaagc 900
gacatttttc gaactcacaa tcgcgaaagt taaagttgtc aagctgtatt cgttcaaata 960
tacaattgta ataatttaca cctgataatt cactttcact gaggtgtcgt tttgtgattc 1020
ttttttctat tatatccatc tgtttcctga aggaattata gtttcaacag atcttgatgc 1080
aatgggctca tttcagaata tttgccagaa ccgttatgat gtcggcgcaa aaaacattat 1140
ccagaacggg agtgcgcctt gagcgacacg aattatgcag tgatttacga cctgcacagc 1200
cataccacag cttccgatgg ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgat 1260
aagcccggat cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 1320
gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 1380
gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 1440
agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 1500
aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 1560
agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 1620
cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 1680
accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 1740
aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 1800
ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 1860
cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 1920
gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 1980
tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 2040
agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 2100
tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2160
caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 2220
ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 2280
gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 2340
gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 2400
gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 2460
aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 2520
ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 2580
acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 2640
ttgtgagggt aaacaactgg cggtatggat ctgcagggcg ggaccagaga aaaatcactc 2700
agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc 2760
atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac 2820
tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc 2880
agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc 2940
aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtgggg 3000
ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga 3060
cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg 3120
tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc 3180
ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg 3240
cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg 3300
cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg 3360
gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 3420
gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc 3480
ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg 3540
cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg 3600
tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc 3660
attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca 3720
ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc 3780
gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc 3840
gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc 3900
agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt 3960
gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca 4020
atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga 4080
agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc 4140
acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg 4200
tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt 4260
tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc 4320
gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca 4380
ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga 4440
ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc 4500
gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt 4560
gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag 4620
tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa 4680
gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc 4740
tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgtga tctcgatccc 4800
gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa 4860
ataattttgt ttaactttaa gaaggagata ta 4892
<210> 706
<211> 185
<212> PRT
<213> 人工序列
<220>
<223> MccE蛋白的C末端部分
<400> 706
Phe Arg Lys Tyr Asp Val Ser Leu Thr Pro Ser Gly Ile Lys Val Asn
1 5 10 15
Asp Glu Ile Thr Leu Leu Tyr Pro Ala Leu Lys Tyr Ala Glu Glu Leu
20 25 30
Tyr Leu Leu Ile Asn Gln Asn Lys Ile Asn Phe Ile Lys Ser Met Ala
35 40 45
Trp Pro Ala Phe Val Asn Asn Ile Ser Asp Ser Val Ser Phe Ile Glu
50 55 60
Gln Ser Met Ile Asp Asn Gln Asn Glu Lys Ala Leu Ile Leu Phe Ile
65 70 75 80
Lys Tyr Lys Thr Lys Ile Ala Gly Val Val Ser Phe Asn Ile Ile Asp
85 90 95
His Ala Asn Lys Thr Ala Tyr Ile Gly Tyr Trp Leu Gly Ala Asn Phe
100 105 110
Gln Gly Lys Gly Ile Val Thr Asn Ala Ile Asn Lys Leu Ile Gln Glu
115 120 125
Tyr Gly Asp Ser Gly Val Ile Lys Arg Phe Val Ile Lys Cys Ile Val
130 135 140
Asp Asn Lys Lys Ser Asn Ala Thr Ala Leu Arg Cys Gly Phe Thr Leu
145 150 155 160
Glu Gly Val Leu Gln Lys Ala Glu Ile Leu Asn Gly Val Ser Tyr Asp
165 170 175
Gln Asn Ile Tyr Ser Lys Val Ile Gly
180 185
<210> 707
<211> 247
<212> DNA
<213> 人工序列
<220>
<223> P24启动子
<400> 707
ttatcgactg cacggtgcac caatgcttct ggcgtcaggc agccatcgga agctgtggta 60
tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct 120
ggataatgtt ttttgcgccg acatcataac ggttctggca aatattctga aatgagccca 180
ttgcatcaag atctgttgaa actataattc cttttgtgag cggataacaa tttcacacag 240
gaaacag 247
<210> 708
<211> 152
<212> DNA
<213> 人工序列
<220>
<223> Proc启动子
<400> 708
cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata 60
actttacggg catggataag gctcgtatga tatattcagg gagaccacaa cggtttccct 120
ctacaaataa ttttgtttaa cttttactag ag 152
<210> 709
<211> 237
<212> DNA
<213> 大肠杆菌
<220>
<223> Cvi
<400> 709
atggatagaa aaagaacaaa attagagttg ttatttgcat ttataataaa tgccaccgca 60
atatatattg cattagctat atatgattgt gtttttagag gaaaggactt tttatccatg 120
catacatttt gcttctctgc attaatgtct gcaatatgtt actttgttgg tgataattat 180
tattcaatat ccgataagat aaaaaggaga tcatatgaga actctgactc taaatga 237
<210> 710
<211> 1328
<212> DNA
<213> 人工序列
<220>
<223> proC-McbG-C-末端MccE
<400> 710
agatctcaca gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc 60
tggataactt tacgggcatg gataaggctc gtatgatata ttcagggaga ccacaacggt 120
ttccctctac aaataatttt gtttaacttt tactagagaa agaggagaaa gctagcatgg 180
atataataga aaaaagaatc acaaaacgac acctcagtga aagtgaatta tcaggtgtaa 240
attattacaa ttgtatattt gaacgaatac agcttgacaa ctttaacttt cgcgattgtg 300
agttcgaaaa atgtcgcttc gtaaactgct caataaagaa tttaaaactt aattttttta 360
aacttattga ctgtgagttc aaagattgtt tattacaagg agtaaatgca gcagacatta 420
tgtttccctg tactttttct cttgtaaatt gtgatttgag atttgttgat tttattagtt 480
tacggttaca gaaatctatt tttttatcct gtcgcttccg tgattgttta tttgaggaaa 540
cagaccttcg taaatctgat tttactggtt cggagtttaa taatacagag ttcagacatt 600
ctgatttgtc tcactgtgat ttttcaatga cagaaggtct cgatataaat cctgagataa 660
acaggatctt atctataaag ataccacagg aagcaggttt aaaaatactt aaacgaatgg 720
gagtggttgt agggggatga cacgtgaagg aggagctaga tgttccggaa gtatgatgta 780
tctcttacgc catcaggaat aaaagtaaat gatgagatta cattattgta tccagcgtta 840
aaatacgctg aagagttata tttgctcatt aatcaaaaca aaataaattt cattaaaagc 900
atggcatggc cagcgtttgt taataacatt tctgattctg tttctttcat tgagcaatca 960
atgattgata accagaatga aaaagcatta atcctcttta ttaaatacaa aactaagata 1020
gctggcgttg tatcttttaa tattattgac catgccaata aaacagcata tattggctat 1080
tggttaggcg ctaactttca gggaaagggg attgtaacca atgctataaa taaactgata 1140
caggagtatg gggattcggg cgttataaaa agatttgtta taaaatgtat tgttgataat 1200
aaaaaaagta atgccacggc attgaggtgt ggcttcacct tagagggtgt tctgcaaaaa 1260
gcagaaatac tcaacggtgt atcatacgat caaaatattt attcgaaagt aattggttaa 1320
gtttaaac 1328
<210> 711
<211> 1002
<212> DNA
<213> 人工序列
<220>
<223> proC-Cvi-C-末端 MccE
<400> 711
agatctcaca gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc 60
tggataactt tacgggcatg gataaggctc gtatgatata ttcagggaga ccacaacggt 120
ttccctctac aaataatttt gtttaacttt tactagagaa agaggagaaa gctagcatgg 180
atagaaaaag aacaaaatta gagttgttat ttgcatttat aataaatgcc accgcaatat 240
atattgcatt agctatatat gattgtgttt ttagaggaaa ggacttttta tccatgcata 300
cattttgctt ctctgcatta atgtctgcaa tatgttactt tgttggtgat aattattatt 360
caatatccga taagataaaa aggagatcct acgagaactc tgactctaaa taagtttgtg 420
aaggaggagc tagatgttcc ggaagtatga tgtatctctt acgccatcag gaataaaagt 480
aaatgatgag attacattat tgtatccagc gttaaaatac gctgaagagt tatatttgct 540
cattaatcaa aacaaaataa atttcattaa aagcatggca tggccagcgt ttgttaataa 600
catttctgat tctgtttctt tcattgagca atcaatgatt gataaccaga atgaaaaagc 660
attaatcctc tttattaaat acaaaactaa gatagctggc gttgtatctt ttaatattat 720
tgaccatgcc aataaaacag catatattgg ctattggtta ggcgctaact ttcagggaaa 780
ggggattgta accaatgcta taaataaact gatacaggag tatggggatt cgggcgttat 840
aaaaagattt gttataaaat gtattgttga taataaaaaa agtaatgcca cggcattgag 900
gtgtggcttc accttagagg gtgttctgca aaaagcagaa atactcaacg gtgtatcata 960
cgatcaaaat atttattcga aagtaattgg ttaagtttaa ac 1002
<210> 712
<211> 5290
<212> DNA
<213> 人工序列
<220>
<223> 载体 pBACT5.0
<400> 712
catatgcgaa ttcgcggccg caaagcttgc ccgggatccc accaccacca ccaccactga 60
gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa 120
taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga 180
ggaactatat ccggattcag gagagcgttc accgacaaac aacagataaa acgaaaggcc 240
cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcatg 300
gggagacccc acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc 360
aggtgggacc accgcgctac tgccgccagg caaattctgt tttatcagac cgcttctgcg 420
ttctgattta atctgtatca ggctgaaaat cttctctcat ccgccaaaac agtttaaact 480
taaccaatta ctttcgaata aatattttga tcgtatgata caccgttgag tatttctgct 540
ttttgcagaa caccctctaa ggtgaagcca cacctcaatg ccgtggcatt acttttttta 600
ttatcaacaa tacattttat aacaaatctt tttataacgc ccgaatcccc atactcctgt 660
atcagtttat ttatagcatt ggttacaatc ccctttccct gaaagttagc gcctaaccaa 720
tagccaatat atgctgtttt attggcatgg tcaataatat taaaagatac aacgccagct 780
atcttagttt tgtatttaat aaagaggatt aatgcttttt cattctggtt atcaatcatt 840
gattgctcaa tgaaagaaac agaatcagaa atgttattaa caaacgctgg ccatgccatg 900
cttttaatga aatttatttt gttttgatta atgagcaaat ataactcttc agcgtatttt 960
aacgctggat acaataatgt aatctcatca tttactttta ttcctgatgg cgtaagagat 1020
acatcatact tccggaacat ctagctcctc cttcacaaac ttatttagag tcagagttct 1080
cgtaggatct cctttttatc ttatcggata ttgaataata attatcacca acaaagtaac 1140
atattgcaga cattaatgca gagaagcaaa atgtatgcat ggataaaaag tcctttcctc 1200
taaaaacaca atcatatata gctaatgcaa tatatattgc ggtggcattt attataaatg 1260
caaataacaa ctctaatttt gttctttttc tatccatgct agctttctcc tctttctcta 1320
gtaaaagtta aacaaaatta tttgtagagg gaaaccgttg tggtctccct gaatatatca 1380
tacgagcctt atccatgccc gtaaagttat ccagcaacca ctcatagacc tagggcagca 1440
gatagggacg acgtggtgtt agctgtgaga tcttgatgca atgggctcat ttcagaatat 1500
ttgccagaac cgttatgatg tcggcgcaaa aaacattatc cagaacggga gtgcgccttg 1560
agcgacacga attatgcagt gatttacgac ctgcacagcc ataccacagc ttccgatggc 1620
tgcctgacgc cagaagcatt ggtgcaccgt gcagtcgata agcccggatc gttccactga 1680
gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 1740
atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 1800
gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 1860
gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 1920
tacctcgctc tgctaatctt gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 1980
accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 2040
ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 2100
cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 2160
agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 2220
ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 2280
tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 2340
ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 2400
cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 2460
gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg 2520
tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat 2580
agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca 2640
cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 2700
acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 2760
acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc 2820
tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct 2880
gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta 2940
agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat 3000
acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc 3060
ggtatggatc tgcagggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg 3120
ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga 3180
acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga 3240
agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc 3300
gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg 3360
tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg 3420
cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt 3480
gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt 3540
cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga 3600
agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg 3660
ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta 3720
cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 3780
attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt 3840
tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag 3900
agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg 3960
gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata 4020
tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga 4080
tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt 4140
tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg 4200
cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg 4260
cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc 4320
gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga 4380
aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc 4440
ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta 4500
caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg 4560
gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg 4620
gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg 4680
taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg 4740
gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg 4800
tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat 4860
gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt 4920
atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc 4980
cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc 5040
caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc 5100
atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc 5160
cacgatgcgt ccggcgtaga ggatcggatc tcgatcccgc gaaattaata cgactcacta 5220
taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga 5280
aggagatata 5290
<210> 713
<211> 5616
<212> DNA
<213> 人工序列
<220>
<223> 载体 pBACT2.0
<400> 713
catatgcgaa ttcgcggccg caaagcttgc ccgggatccc accaccacca ccaccactga 60
gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa 120
taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga 180
ggaactatat ccggattcag gagagcgttc accgacaaac aacagataaa acgaaaggcc 240
cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcatg 300
gggagacccc acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc 360
aggtgggacc accgcgctac tgccgccagg caaattctgt tttatcagac cgcttctgcg 420
ttctgattta atctgtatca ggctgaaaat cttctctcat ccgccaaaac agtttaaact 480
taaccaatta ctttcgaata aatattttga tcgtatgata caccgttgag tatttctgct 540
ttttgcagaa caccctctaa ggtgaagcca cacctcaatg ccgtggcatt acttttttta 600
ttatcaacaa tacattttat aacaaatctt tttataacgc ccgaatcccc atactcctgt 660
atcagtttat ttatagcatt ggttacaatc ccctttccct gaaagttagc gcctaaccaa 720
tagccaatat atgctgtttt attggcatgg tcaataatat taaaagatac aacgccagct 780
atcttagttt tgtatttaat aaagaggatt aatgcttttt cattctggtt atcaatcatt 840
gattgctcaa tgaaagaaac agaatcagaa atgttattaa caaacgctgg ccatgccatg 900
cttttaatga aatttatttt gttttgatta atgagcaaat ataactcttc agcgtatttt 960
aacgctggat acaataatgt aatctcatca tttactttta ttcctgatgg cgtaagagat 1020
acatcatact tccggaacat ctagctcctc cttcacgtgt catcccccta caaccactcc 1080
cattcgttta agtattttta aacctgcttc ctgtggtatc tttatagata agatcctgtt 1140
tatctcagga tttatatcga gaccttctgt cattgaaaaa tcacagtgag acaaatcaga 1200
atgtctgaac tctgtattat taaactccga accagtaaaa tcagatttac gaaggtctgt 1260
ttcctcaaat aaacaatcac ggaagcgaca ggataaaaaa atagatttct gtaaccgtaa 1320
actaataaaa tcaacaaatc tcaaatcaca atttacaaga gaaaaagtac agggaaacat 1380
aatgtctgct gcatttactc cttgtaataa acaatctttg aactcacagt caataagttt 1440
aaaaaaatta agttttaaat tctttattga gcagtttacg aagcgacatt tttcgaactc 1500
acaatcgcga aagttaaagt tgtcaagctg tattcgttca aatatacaat tgtaataatt 1560
tacacctgat aattcacttt cactgaggtg tcgttttgtg attctttttt ctattatatc 1620
catgctagct ttctcctctt tctctagtaa aagttaaaca aaattatttg tagagggaaa 1680
ccgttgtggt ctccctgaat atatcatacg agccttatcc atgcccgtaa agttatccag 1740
caaccactca tagacctagg gcagcagata gggacgacgt ggtgttagct gtgagatctt 1800
gatgcaatgg gctcatttca gaatatttgc cagaaccgtt atgatgtcgg cgcaaaaaac 1860
attatccaga acgggagtgc gccttgagcg acacgaatta tgcagtgatt tacgacctgc 1920
acagccatac cacagcttcc gatggctgcc tgacgccaga agcattggtg caccgtgcag 1980
tcgataagcc cggatcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 2040
cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 2100
cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 2160
tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 2220
tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcttgtta ccagtggctg 2280
ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 2340
aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 2400
cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 2460
ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 2520
agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 2580
ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 2640
acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 2700
cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 2760
gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga 2820
tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct 2880
cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt 2940
gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 3000
tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 3060
cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg 3120
tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc 3180
tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc 3240
tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg 3300
atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg 3360
gaacgttgtg agggtaaaca actggcggta tggatctgca gggcgggacc agagaaaaat 3420
cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca 3480
gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc 3540
cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt 3600
tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt 3660
aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg 3720
tggggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc 3780
agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat 3840
catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac 3900
ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc 3960
ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc 4020
cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca 4080
gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 4140
tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat 4200
tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca 4260
gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg 4320
tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg 4380
cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc 4440
cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc 4500
gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca 4560
gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg 4620
cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga 4680
tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca 4740
cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg 4800
cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca 4860
ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg 4920
gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca 4980
gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt 5040
cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag 5100
agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga 5160
attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg 5220
tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt 5280
aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc 5340
aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc 5400
ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc 5460
gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cggatctcga 5520
tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct 5580
agaaataatt ttgtttaact ttaagaagga gatata 5616
<210> 714
<211> 5988
<212> DNA
<213> 人工序列
<220>
<223> 载体pBACT5.0-mCherry
<400> 714
aataattttg tttaacttta agaaggagat ataccatggt gagcaagggc gaggaggata 60
acatggctat cattaaagag ttcatgcgct tcaaagttca catggagggt tctgttaacg 120
gtcacgagtt cgagatcgaa ggcgaaggcg agggccgtcc gtatgaaggc acccagaccg 180
ccaaactgaa agtgactaaa ggcggcccgc tgccttttgc gtgggacatc ctgagcccgc 240
aatttatgta cggttctaaa gcttatgtta aacacccagc ggatatcccg gactatctga 300
agctgtcttt tccggaaggt ttcaagtggg aacgcgtaat gaattttgaa gatggtggtg 360
tcgtgaccgt cactcaggac tcctccctgc aggatggcga gttcatctat aaagttaaac 420
tgcgtggtac taattttcca tctgatggcc cggtgatgca gaagaagacg atgggttggg 480
aggcgtctag cgaacgcatg tatccggaag atggtgcgct gaaaggcgaa attaaacagc 540
gcctgaaact gaaagatggc ggccattatg acgctgaagt gaaaaccacg tacaaagcca 600
agaaacctgt gcagctgcct ggcgcgtaca atgtgaatat taaactggac atcacctctc 660
ataatgaaga ttatacgatc gtagagcaat atgagcgcgc ggagggtcgt cattctaccg 720
gtggcatgga cgagctgtac aagcaccacc accaccacca ctgaggatcc caccaccacc 780
accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca 840
ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt 900
tgctgaaagg aggaactata tccggattca ggagagcgtt caccgacaaa caacagataa 960
aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct 1020
actctcgcat ggggagaccc cacactacca tcggcgctac ggcgtttcac ttctgagttc 1080
ggcatggggt caggtgggac caccgcgcta ctgccgccag gcaaattctg ttttatcaga 1140
ccgcttctgc gttctgattt aatctgtatc aggctgaaaa tcttctctca tccgccaaaa 1200
cagtttaaac ttaaccaatt actttcgaat aaatattttg atcgtatgat acaccgttga 1260
gtatttctgc tttttgcaga acaccctcta aggtgaagcc acacctcaat gccgtggcat 1320
tacttttttt attatcaaca atacatttta taacaaatct ttttataacg cccgaatccc 1380
catactcctg tatcagttta tttatagcat tggttacaat cccctttccc tgaaagttag 1440
cgcctaacca atagccaata tatgctgttt tattggcatg gtcaataata ttaaaagata 1500
caacgccagc tatcttagtt ttgtatttaa taaagaggat taatgctttt tcattctggt 1560
tatcaatcat tgattgctca atgaaagaaa cagaatcaga aatgttatta acaaacgctg 1620
gccatgccat gcttttaatg aaatttattt tgttttgatt aatgagcaaa tataactctt 1680
cagcgtattt taacgctgga tacaataatg taatctcatc atttactttt attcctgatg 1740
gcgtaagaga tacatcatac ttccggaaca tctagctcct ccttcacaaa cttatttaga 1800
gtcagagttc tcgtaggatc tcctttttat cttatcggat attgaataat aattatcacc 1860
aacaaagtaa catattgcag acattaatgc agagaagcaa aatgtatgca tggataaaaa 1920
gtcctttcct ctaaaaacac aatcatatat agctaatgca atatatattg cggtggcatt 1980
tattataaat gcaaataaca actctaattt tgttcttttt ctatccatgc tagctttctc 2040
ctctttctct agtaaaagtt aaacaaaatt atttgtagag ggaaaccgtt gtggtctccc 2100
tgaatatatc atacgagcct tatccatgcc cgtaaagtta tccagcaacc actcatagac 2160
ctagggcagc agatagggac gacgtggtgt tagctgtgag atcttgatgc aatgggctca 2220
tttcagaata tttgccagaa ccgttatgat gtcggcgcaa aaaacattat ccagaacggg 2280
agtgcgcctt gagcgacacg aattatgcag tgatttacga cctgcacagc cataccacag 2340
cttccgatgg ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgat aagcccggat 2400
cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 2460
ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 2520
tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 2580
taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 2640
caccgcctac atacctcgct ctgctaatct tgttaccagt ggctgctgcc agtggcgata 2700
agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 2760
gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 2820
gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 2880
ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 2940
acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 3000
tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 3060
ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 3120
ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 3180
ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc 3240
ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc 3300
tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct 3360
gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca 3420
tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg 3480
tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat 3540
tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat 3600
gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat 3660
gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg 3720
atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt 3780
aaacaactgg cggtatggat ctgcagggcg ggaccagaga aaaatcactc agggtcaatg 3840
ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat 3900
gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac 3960
acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc 4020
gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca 4080
gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtgggg ccgccatgcc 4140
ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg 4200
agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca 4260
gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg 4320
catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa 4380
ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt 4440
gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 4500
gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 4560
ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct 4620
ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct 4680
gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca 4740
ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca 4800
gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt 4860
gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct 4920
gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag 4980
aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca 5040
cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag 5100
agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct 5160
ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca 5220
ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac 5280
ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca 5340
gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc 5400
ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag 5460
aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact 5520
ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg 5580
ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga 5640
cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg 5700
agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc 5760
acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc 5820
cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg 5880
gtgatgccgg ccacgatgcg tccggcgtag aggatcggat ctcgatcccg cgaaattaat 5940
acgactcact ataggggaat tgtgagcgga taacaattcc cctctaga 5988
<210> 715
<211> 2353
<212> DNA
<213> 人工序列
<220>
<223> McbG-MccE
<400> 715
atggatataa tagaaaaaag aatcacaaaa cgacacctca gtgaaagtga attatcaggt 60
gtaaattatt acaattgtat atttgaacga atacagcttg acaactttaa ctttcgcgat 120
tgtgagttcg aaaaatgtcg cttcgtaaac tgctcaataa agaatttaaa acttaatttt 180
tttaaactta ttgactgtga attcaaagat tgtttattac aaggagtaaa tgcagcagac 240
attatgtttc cctgtacttt ttctcttgta aattgtgatt tgagatttgt tgattttatt 300
agtttacggt tacagaaatc tattttttta tcctgtcgct tccgtgattg tttatttgag 360
gaaacagacc ttcgtaaatc tgattttact ggttcggagt ttaataatac agagttcaga 420
cattctgatt tgtctcactg tgatttttca atgacagaag gtctcgatat aaatcctgag 480
ataaacagga tcttatctat aaagatacca caggaagcag gtttaaaaat acttaaacga 540
atgggagtgg ttgtaggggg atgacacgtg aaggaggagc tagatggcgc aaaaaataac 600
accatctaaa attgttaaac atgcccggga gttaattatt aaggggattg aatcgggcga 660
taactcattt gtcatttttg atgtagatgg agctctggag cgtctggaac attacaggtg 720
tcaacttaag agttttttcc ccaacagcag tatcgcatat tcatataagt caaataatct 780
ggcacaatgg tgccagatta tctcaggtaa aggtttgtat gcagaggtgt gctctgttga 840
tgaaatgaac ctcgctaaaa gagacggttt taaccggatt gtttttgatg gccctttaaa 900
aaaaacgagt gaactattaa aggcaataga gattggggca ttaattgaag ttgacaatat 960
tgatgaatgt aaaagactta atgaactatg taaattgcat aagttgacat gtagaattca 1020
tttgagatta tcgcattact atgatgataa tttatcaaga tttggcttat ctgaaagtga 1080
ggctattaat ttactggaga tgttgatcag taagtcagag tatctcattt tagacgggtt 1140
ccatttacat gttggctcta atctacccaa tgcggaaaaa atatgtaaag ctgttataca 1200
gtatcatgag ttgattctta ggtatatgcc agatgatggc actcttaatc ttggtagtgg 1260
gatacctgca gattctttct cagcatccag tgataaccca actccatgtc cggaggtatt 1320
tttctcatcg atatatgata caataaaaaa ttgcttcggt actgtatgtg ataaatggaa 1380
ctatattttt gaacctggga gacatctggt cgaagacttt ggctacttta tagggaaagt 1440
tattagtact aaaaacaggt atggtgttaa agttgctcaa actaacattg gtataaactg 1500
gataccttcg atcagaaatt gggaccattc atttacattg tttcataatc ataaccatat 1560
ttcagatgat aaatctgatg agtatattat tgctggattc aattgctttg aatgtgattg 1620
cttgtttcct tcagttattc ttcccagtaa cttatctgat tatttatttt ctgttcgagg 1680
gtgtggtgct tatgatatgc aaacaggaaa ccagtggacg cgtaatcttt atgctgtata 1740
taccataaca aatgatgttg ttaatatatc aaggattcac agaagagaac ttgatttccg 1800
gaagtatgat gtatctctta cgccatcagg aataaaagta aatgatgaga ttacattatt 1860
gtatccagcg ttaaaatacg ctgaagagtt atatttgctc attaatcaaa acaaaataaa 1920
tttcattaaa agcatggcat ggccagcgtt tgttaataac atttctgatt ctgtttcttt 1980
cattgagcaa tcaatgattg ataaccagaa tgaaaaagca ttaatcctct ttattaaata 2040
caaaactaag atagctggcg ttgtatcttt taatattatt gaccatgcca ataaaacagc 2100
atatattggc tattggttag gcgctaactt tcagggaaag gggattgtaa ccaatgctat 2160
aaataaactg atacaggagt atggggattc gggcgttata aaaagatttg ttataaaatg 2220
tattgttgat aataaaaaaa gtaatgccac ggcattgagg tgtggcttca ccttagaggg 2280
tgttctgcaa aaagcagaaa tactcaacgg tgtatcatac gatcaaaata tttattcgaa 2340
agtaattggt taa 2353
<210> 716
<211> 1144
<212> DNA
<213> 人工序列
<220>
<223> McbG-Cter部分MccE
<400> 716
atggatataa tagaaaaaag aatcacaaaa cgacacctca gtgaaagtga attatcaggt 60
gtaaattatt acaattgtat atttgaacga atacagcttg acaactttaa ctttcgcgat 120
tgtgagttcg aaaaatgtcg cttcgtaaac tgctcaataa agaatttaaa acttaatttt 180
tttaaactta ttgactgtga attcaaagat tgtttattac aaggagtaaa tgcagcagac 240
attatgtttc cctgtacttt ttctcttgta aattgtgatt tgagatttgt tgattttatt 300
agtttacggt tacagaaatc tattttttta tcctgtcgct tccgtgattg tttatttgag 360
gaaacagacc ttcgtaaatc tgattttact ggttcggagt ttaataatac agagttcaga 420
cattctgatt tgtctcactg tgatttttca atgacagaag gtctcgatat aaatcctgag 480
ataaacagga tcttatctat aaagatacca caggaagcag gtttaaaaat acttaaacga 540
atgggagtgg ttgtaggggg atgacacgtg aaggaggagc tagatgttcc ggaagtatga 600
tgtatctctt acgccatcag gaataaaagt aaatgatgag attacattat tgtatccagc 660
gttaaaatac gctgaagagt tatatttgct cattaatcaa aacaaaataa atttcattaa 720
aagcatggca tggccagcgt ttgttaataa catttctgat tctgtttctt tcattgagca 780
atcaatgatt gataaccaga atgaaaaagc attaatcctc tttattaaat acaaaactaa 840
gatagctggc gttgtatctt ttaatattat tgaccatgcc aataaaacag catatattgg 900
ctattggtta ggcgctaact ttcagggaaa ggggattgta accaatgcta taaataaact 960
gatacaggag tatggggatt cgggcgttat aaaaagattt gttataaaat gtattgttga 1020
taataaaaaa agtaatgcca cggcattgag gtgtggcttc accttagagg gtgttctgca 1080
aaaagcagaa atactcaacg gtgtatcata cgatcaaaat atttattcga aagtaattgg 1140
ttaa 1144
<210> 717
<211> 2026
<212> DNA
<213> 人工序列
<220>
<223> Cvi-MccE
<400> 717
atggatagaa aaagaacaaa attagagttg ttatttgcat ttataataaa tgccaccgca 60
atatatattg cattagctat atatgattgt gtttttagag gaaaggactt tttatccatg 120
catacatttt gcttctctgc attaatgtct gcaatatgtt actttgttgg tgataattat 180
tattcaatat ccgataagat aaaaaggaga tcctacgaga actctgactc taaataacac 240
gtgaaggagg agctagatgg cgcaaaaaat aacaccatct aaaattgtta aacatgcccg 300
ggagttaatt attaagggga ttgaatcggg cgataactca tttgtcattt ttgatgtaga 360
tggagctctg gagcgtctgg aacattacag gtgtcaactt aagagttttt tccccaacag 420
cagtatcgca tattcatata agtcaaataa tctggcacaa tggtgccaga ttatctcagg 480
taaaggtttg tatgcagagg tgtgctctgt tgatgaaatg aacctcgcta aaagagacgg 540
ttttaaccgg attgtttttg atggcccttt aaaaaaaacg agtgaactat taaaggcaat 600
agagattggg gcattaattg aagttgacaa tattgatgaa tgtaaaagac ttaatgaact 660
atgtaaattg cataagttga catgtagaat tcatttgaga ttatcgcatt actatgatga 720
taatttatca agatttggct tatctgaaag tgaggctatt aatttactgg agatgttgat 780
cagtaagtca gagtatctca ttttagacgg gttccattta catgttggct ctaatctacc 840
caatgcggaa aaaatatgta aagctgttat acagtatcat gagttgattc ttaggtatat 900
gccagatgat ggcactctta atcttggtag tgggatacct gcagattctt tctcagcatc 960
cagtgataac ccaactccat gtccggaggt atttttctca tcgatatatg atacaataaa 1020
aaattgcttc ggtactgtat gtgataaatg gaactatatt tttgaacctg ggagacatct 1080
ggtcgaagac tttggctact ttatagggaa agttattagt actaaaaaca ggtatggtgt 1140
taaagttgct caaactaaca ttggtataaa ctggatacct tcgatcagaa attgggacca 1200
ttcatttaca ttgtttcata atcataacca tatttcagat gataaatctg atgagtatat 1260
tattgctgga ttcaattgct ttgaatgtga ttgcttgttt ccttcagtta ttcttcccag 1320
taacttatct gattatttat tttctgttcg agggtgtggt gcttatgata tgcaaacagg 1380
aaaccagtgg acgcgtaatc tttatgctgt atataccata acaaatgatg ttgttaatat 1440
atcaaggatt cacagaagag aacttgattt ccggaagtat gatgtatctc ttacgccatc 1500
aggaataaaa gtaaatgatg agattacatt attgtatcca gcgttaaaat acgctgaaga 1560
gttatatttg ctcattaatc aaaacaaaat aaatttcatt aaaagcatgg catggccagc 1620
gtttgttaat aacatttctg attctgtttc tttcattgag caatcaatga ttgataacca 1680
gaatgaaaaa gcattaatcc tctttattaa atacaaaact aagatagctg gcgttgtatc 1740
ttttaatatt attgaccatg ccaataaaac agcatatatt ggctattggt taggcgctaa 1800
ctttcaggga aaggggattg taaccaatgc tataaataaa ctgatacagg agtatgggga 1860
ttcgggcgtt ataaaaagat ttgttataaa atgtattgtt gataataaaa aaagtaatgc 1920
cacggcattg aggtgtggct tcaccttaga gggtgttctg caaaaagcag aaatactcaa 1980
cggtgtatca tacgatcaaa atatttattc gaaagtaatt ggttaa 2026
<210> 718
<211> 817
<212> DNA
<213> 人工序列
<220>
<223> Cvi-Cter部分MccE
<400> 718
atggatagaa aaagaacaaa attagagttg ttatttgcat ttataataaa tgccaccgca 60
atatatattg cattagctat atatgattgt gtttttagag gaaaggactt tttatccatg 120
catacatttt gcttctctgc attaatgtct gcaatatgtt actttgttgg tgataattat 180
tattcaatat ccgataagat aaaaaggaga tcctacgaga actctgactc taaataacac 240
gtgaaggagg agctagatgt tccggaagta tgatgtatct cttacgccat caggaataaa 300
agtaaatgat gagattacat tattgtatcc agcgttaaaa tacgctgaag agttatattt 360
gctcattaat caaaacaaaa taaatttcat taaaagcatg gcatggccag cgtttgttaa 420
taacatttct gattctgttt ctttcattga gcaatcaatg attgataacc agaatgaaaa 480
agcattaatc ctctttatta aatacaaaac taagatagct ggcgttgtat cttttaatat 540
tattgaccat gccaataaaa cagcatatat tggctattgg ttaggcgcta actttcaggg 600
aaaggggatt gtaaccaatg ctataaataa actgatacag gagtatgggg attcgggcgt 660
tataaaaaga tttgttataa aatgtattgt tgataataaa aaaagtaatg ccacggcatt 720
gaggtgtggc ttcaccttag agggtgttct gcaaaaagca gaaatactca acggtgtatc 780
atacgatcaa aatatttatt cgaaagtaat tggttaa 817
<210> 719
<211> 9521
<212> DNA
<213> 人工序列
<220>
<223> 载体pUC-ColV
<400> 719
gcgatcgcgg ctcccgacat cttggaccat tagctccaca ggtatcttct tccctctagt 60
ggtcataaca gcagcttcag ctacctctca attcaaaaaa cccctcaaga cccgtttaga 120
ggccccaagg ggttatgcta tcaatcgttg cgttacacac acaaaaaacc aacacacatc 180
catcttcgat ggatagcgat tttattatct aactgctgat cgagtgtagc cagatctttg 240
tcgatcctac catccactcg acacacccgc cagcggccgc tgccaagctt ccgagctctg 300
cccttcccta gagaatcctg ccaggcttgc cacactgata tatcttgact ttatgtaaac 360
gatatgacac tttaacatga taatgattac cattctcttt taatatacag agaaactagg 420
aaatagatga atgagttatg ttactttaat attctctgac aataacctaa atcagttaga 480
ttattgtcat ttaataaata atgacattct ttcatcataa ataaaaagac tattgtttat 540
aatattgttc tcagcattat atgattattt atcctgataa ctctcctatg ttgtatgttt 600
atatgatttt ccttgaaaca tataatgcaa attttcgatt tattttccat cattaatcca 660
gataaacaac aaactaatag tatgcaagga gacattattt gtttcgccat gatgctttag 720
aaaacagaaa aatgaagtgg cagggacggg caatattact tcccggaata ccactgtggt 780
taatcatgct gggaagcatt gtgtttatta cggcatttct gatgttcatt attgttggta 840
cctatagccg ccgtgttaat gtcagtggtg aggtcacaac ctggccaaga gctgtcaata 900
tatattcagg tgtacaggga tttgttgtca ggcagtttgt tcatgaaggg cagttgataa 960
aaaaagggga tcctgtttat ctgattgaca tcagtaaaag tacacgcaat ggtattgtca 1020
ctgataatca tcgccgggat atagaaaacc agctggttcg tgtggacaac attatttccc 1080
gtctggaaga aagtaaaaaa ataacgctag ataccctgga aaaacaacgt ctgcaataca 1140
cagatgcgtt ccgtcgctca tcagacatta tacagcgtgc agaggaaggg ataaaaataa 1200
tgaaaaataa tatggagaat tacagatact atcagtcaaa aggactgatt aataaagatc 1260
aattaactaa ccaagttgca ttatattatc aacaacaaaa caaccttctc agtctgagcg 1320
gacaaaatga acaaaatgcc ctgcagataa ccactctgga gagtcagatt cagactcagg 1380
cagcagattt tgataatcgt atctatcaga tggaactgca acgactcgaa ttgcagaaag 1440
aactggttaa cactgatgtg gaaggcgaaa tcattatccg ggcgttgtct gacgggaaag 1500
ttgactccct gagtgtcact gtagggcaaa tggtcaatac cggagacagc cttctgcagg 1560
ttattcctga gaacattgaa aactattatc ttattctctg ggtcccgaat gatgctgttc 1620
cttatatttc ggctggtgac aaagtgaata ttcgttatga agccttcccc tcagaaaaat 1680
ttgggcagtt ctctgctacg gttaaaacta tatccaggac tcctgcgtca acacaggaaa 1740
tgttgaccta taagggagca cctcaaaata cgccgggtgc ctctgttccc tggtataaag 1800
tcattgcgac gcctgaaaag cagataatca ggtatgacga aaaatacctc cctctggaaa 1860
atggaatgaa agccgaaagt acactatttc tggaaaaaag gcgtatttac cagtggatgc 1920
tttctccttt ctatgacatg aaacacagtg caacaggacc gatcaatgac taacaggaat 1980
ttcagacaaa ttataaatct gcttgatttg cgctggcaac gtcgtgttcc ggttattcat 2040
cagacggaga ccgctgaatg tggactggcc tgcctagcaa tgatatgcgg tcattttggt 2100
aagaatattg acctgatata tcttcgccgg aagtttaatc tctctgcccg tggagcaacc 2160
cttgcaggaa tcaatggaat agcggagcaa ctggggatgg ccacccgggc tctttcactg 2220
gagttggatg aacttcgagt cctcaaaacg ccgtgtattc tccactggga tttcagtcac 2280
ttcgtcgttc tggtcagcgt aaagcgtaac cgttatgtac tgcatgatcc ggccaggggc 2340
ataagatata tcagccggga ggaaatgagc cgatatttta caggcgttgc acttgaggtc 2400
tggcccggaa gtgaattcca gtcggaaacc ctgcagaccc gcataagtct tcgttcactg 2460
attaacagta tttacggtat taaaagaacg ctggcgaaaa ttttctgtct gtcagttgta 2520
attgaagcaa tcaatctgct aatgccggtg gggacacagc tggttatgga tcatgctatt 2580
cctgcggggg acagagggct actgacgcta atttctgctg ctcttatgtt ttttatatta 2640
ctcaaagctg caacgagtac gctgcgcgca tggtcttcac tggttatgag cacgctcatc 2700
aatgtacagt ggcagtcggg gctgttcgat catcttctca gactaccgct ggcgtttttt 2760
gaacgccgaa aattaggtga tatccagtca cgttttgact cccttgacac attgagggcc 2820
acatttacca ccagtgtgat cgggttcata atggacagca ttatggttgt cggtgtttgt 2880
gtgatgatgc tgttatacgg aggatatctc acctggatag ttctctgctt taccacaatt 2940
tacattttta ttcgactggt gacatacggc aattaccgac agatatcaga agaatgtctt 3000
gtcagggagg cccgtgccgc ctcctatttt atggaaacat tatatggtat tgccacggta 3060
aaaatccagg ggatggtcgg aattcggggg gcacactggc ttaatatgaa aatagatgcg 3120
ataaattcgg gtattaagct aaccaggatg gatttgctct tcggaggaat aaataccttt 3180
gttaccgcct gtgatcagat tgtaatttta tggctgggag caggccttgt gatcgataat 3240
cagatgacaa taggaatgtt tgtagcgttt agttcttttc gtgggcagtt ttcggaaaga 3300
gttgcctctc tgaccagttt tcttcttcag ctaagaataa tgagtctgca caatgagcgc 3360
attgcagata ttgcattaca tgaaaaggag gaaaagaaac ctgaaattga aatcgttgct 3420
gatatggggc caatatccct ggaaaccaat ggtttaagct atcgttatga cagtcagtca 3480
gcaccgatat tcagtgctct gagtttatct gtagctccgg gggaaagtgt ggctataact 3540
ggtgcttccg gtgcgggaaa aaccacatta atgaaagtac tatgtggact atttgaacct 3600
gatagcggga gggtactgat aaatggtata gatatacgcc aaattggaat aaataattat 3660
caccggatga tagcctgtgt tatgcaggat gaccggctat tttcaggctc aattcgtgaa 3720
aatatctgtg gttttgcaga ggaaatggat gaagagtgga tggtagaatg tgccagagca 3780
agtcatattc atgatgttat aatgaatatg ccaatgggat atgaaacatt aataggtgaa 3840
cttggggaag gtctttctgg cggtcaaaaa cagcgtatat ttattgcacg agccttatac 3900
cggaaaccag gaatattatt tatggatgag gcaaccagtg ctcttgattc agagagtgaa 3960
catttcgtga atgttgccat aaaaaacatg aatatcacca gggtaattat tgcacacaga 4020
gaaacaacgt tgagaactgt tgatagagtt atttctattt aaaccataga ggaattacaa 4080
gcgtatgagg aatatttctt cctgttataa ttcctcgtta tgctcagata tctgttggag 4140
gtggaatgga agatagacaa tccaccaaga agaaatatca ttctgtgtgg attgtccaat 4200
aactgttctt tcttatatta aataatacta tttataaaca aacatcacta agattatttg 4260
gactccaatt acacaatctt cccgcagcat agttccatgc ttctgaaggt atcccttcgg 4320
gtttttgctt aattgttccc cctaaaccgg atggagacat tgcaggatta ggtttgtgag 4380
tggatgcata gtcatatatt gcacctccag ccacaccccc agcagctgct ccaattcctc 4440
ctgcaacaaa ttgcccggat agtgttccta tagccatcgc aatatcacgc cctgaagcac 4500
caccagaaac agaatctaat tcatttagag tcagagttct catatgatct cctttttatc 4560
ttatcggata ttgaataata attatcacca acaaagtaac atattgcaga cattaatgca 4620
gagaagcaaa atgtatgcat ggataaaaag tcctttcctc taaaaacaca atcatatata 4680
gctaatgcaa tatatattgc ggtggcattt attataaatg caaataacaa ctctaatttt 4740
gttctttttc tatccattac tttttatccc attactttct atcccattac cacacaaaca 4800
ctaacgataa tgattatcgt taacatagtc aagagtgaag ggtaggaggc cctcaacccc 4860
ctataagggg tccgcttgga aaacggattt ccccacgtca agagaattga tttgaacgag 4920
tggcttcgct acaaacagct cccgtttgtg tgtgaaaaac ccctctcaac agatctcaac 4980
tctgcacgat atctacagga gattgctcca aaaggcagta tcgtgtcttt taactaccga 5040
tataaccccg tcatcgacat gataatgcgt ctgagaaatg agaagaaatt aggtgaactg 5100
catttcttct cagcagaatt taataaaaat tctgcactga cccgtctaga agttgtctcc 5160
tcctgcactg actgactgat acaatcgatt tctggatccg caggcctctg ctagcttgac 5220
tgactgagat acagcgtacc ttcagctcac agacatgata agatacattg atgagtttgg 5280
acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat 5340
tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca 5400
ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta 5460
caaatgtggt attggcccat ctctatcggt atcgtagcat aaccccttgg ggcctctaaa 5520
cgggtcttga ggggtttttt gtgcccctcg ggccggattg ctatctaccg gcattggcgc 5580
agaaaaaaat gcctgatgcg acgctgcgcg tcttatactc ccacatatgc cagattcagc 5640
aacggatacg gcttccccaa cttgcccact tccatacgtg tcctccttac cagaaattta 5700
tccttaaggt cgtcagctat cctgcaggcg atctctcgat ttcgatcaag acattccttt 5760
aatggtcttt tctggacacc actaggggtc agaagtagtt catcaaactt tcttccctcc 5820
ctaatctcat tggttacctt gggctatcga aacttaatta accagtcaag tcagctactt 5880
ggcgagatcg acttgtctgg gtttcgacta cgctcagaat tgcgtcagtc aagttcgatc 5940
tggtccttgc tattgcaccc gttctccgat tacgagtttc atttaaatca tgtgagcaaa 6000
aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 6060
ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 6120
aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 6180
gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 6240
tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 6300
tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 6360
gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 6420
cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 6480
cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 6540
agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 6600
caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 6660
ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 6720
aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 6780
tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 6840
agcgatctgt ctatttcgtt catccatagt tgcatttaaa tttccgaact ctccaaggcc 6900
ctcgtcggaa aatcttcaaa cctttcgtcc gatccatctt gcaggctacc tctcgaacga 6960
actatcgcaa gtctcttggc cggccttgcg ccttggctat tgcttggcag cgcctatcgc 7020
caggtattac tccaatcccg aatatccgag atcgggatca cccgagagaa gttcaaccta 7080
catcctcaat cccgatctat ccgagatccg aggaatatcg aaatcggggc gcgccaagct 7140
gttgtgaccg cttgctctag ccagctatcg agttgtgaac cgatccatct agcaattggt 7200
ctcgatctag cgataggctt cgatctagct atgtagaaac gccgtgtgct cgatcgcttg 7260
ataaggtcca cgtagctgct ataattgctt caacagaaca tattgactat ccggtattac 7320
ccggcaaagg aggtagccaa catgacagct ctgacagagg gagcgaaact gttcgagaag 7380
gagatcccgt acatcacaga gttggaagga gatgtcgaag ggatgaaatt catcatcaag 7440
ggagaaggaa cgggtgacgc aacgaccgga acgatcaagg ccaaatacat ttgtaccacg 7500
ggcgatttgc ctgtcccctg ggcgacgctt gtaagcacgc tctcgtatgg tgtccagtgc 7560
ttcgcgaaat atccatcgca cattaaggac tttttcaagt cggccatgcc agaaggttac 7620
acacaagaac gaaccatctc ctttgagggg gacggagtgt ataagacacg cgcgatggta 7680
acgtacgagc gcgggtccat ctacaacagg gtaactctta ctggggagaa ctttaagaag 7740
gacgggcata tcttgcggaa aaacgtggca tttcaatgtc cgccctcgat tctgtatatt 7800
ctcccggaca cggtgaataa tgggatcaga gtggagttca accaggcata cgatattgag 7860
ggtgtgactg aaaagctcgt caccaaatgc agccagatga atcgccccct tgcgggatca 7920
gcagccgtcc atatcccccg gtatcaccat attacctacc acacaaaact ctcaaaagac 7980
agagatgaga gaagggacca tatgtgcctg gtcgaagtag tgaaggcggt ggatcttgat 8040
acataccagt aggctagcca ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct 8100
tatactccca catatgccag attcagcaac ggatacggct tccccaactt gcccacttcc 8160
atacgtgtcc tccttaccag aaatttatcc ttaagggcgc gcctggtgta ccgagaacga 8220
tcctctcagt gcgagtctcg acgatccata tcgttgcttg gcagtcagcc agtcggaatc 8280
cagcttggga cccaggaagt ccaatcgtca gatattgtac tcaagcctgg tcacggcagc 8340
gtaccgatct gtttaaacct agatattgat agtctgatcg gtcaacgtat aatcgagtcc 8400
tagcttttgc aaacatctat caagagacag gatcagcagg aggctttcgc atgattgaac 8460
aagatggatt gcacgcaggt tctccggcgg cttgggtgga gaggctattc ggctatgact 8520
gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 8580
gtccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg 8640
cagcgcggct atcgtggctg gcgacgacgg gcgttccttg cgcggctgtg ctcgacgttg 8700
tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 8760
catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 8820
atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 8880
cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 8940
ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgtc tatgcccgac ggcgaggatc 9000
tcgtcgtgac ccacggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 9060
ctggattcat cgactgtggc cgtctgggtg tggcggaccg ctatcaggac atagcgttgg 9120
ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc cttgtgcttt 9180
acggtatcgc cgcgcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 9240
tctgaccgat tctaggtgca ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct 9300
tatactccca catatgccag attcagcaac ggatacggct tccccaactt gcccacttcc 9360
atacgtgtcc tccttaccag aaatttatcc ttaaggtcgt ttaaactcga ctctggctct 9420
atcgaatctc cgtcgtttcg agcttacgcg aacagccgtg gcgctcattt gctcgtcggg 9480
catcgaatct cgtcagctat cgtcagctta cctttttggc a 9521
<210> 720
<211> 9
<212> PRT
<213> 弯曲乳杆菌
<400> 720
Asn Ile Pro Gln Leu Thr Pro Thr Pro
1 5
<210> 721
<211> 27
<212> DNA
<213> 弯曲乳杆菌
<400> 721
aacattccgc agctgacccc gaccccg 27
<210> 722
<211> 18
<212> PRT
<213> 苏云金芽孢杆菌
<220>
<221> 变体
<222> (4)..(4)
<223> Xaa = 任何氨基酸
<220>
<221> 变体
<222> (7)..(7)
<223> Xaa = 任何氨基酸
<220>
<221> 变体
<222> (10)..(10)
<223> Xaa = 任何氨基酸
<400> 722
Asp Trp Thr Xaa Trp Ser Xaa Leu Val Xaa Ala Ala Cys Ser Val Glu
1 5 10 15
Leu Leu
<210> 723
<211> 54
<212> DNA
<213> 苏云金芽孢杆菌
<220>
<221> misc_feature
<222> (10)..(12)
<223> n = A,T,C或G
<220>
<221> misc_feature
<222> (19)..(21)
<223> n = A,T,C或G
<220>
<221> misc_feature
<222> (28)..(30)
<223> n = A,T,C或G
<400> 723
gattggaccn nntggagcnn nctggtgnnn gcggcgtgca gcgtggaact gctg 54
<210> 724
<211> 30
<212> PRT
<213> 弯曲乳杆菌 L442
<400> 724
Ala Tyr Pro Gly Asn Gly Val His Cys Gly Lys Tyr Ser Cys Thr Val
1 5 10 15
Asp Lys Gln Thr Ala Ile Gly Asn Ile Gly Asn Asn Ala Ala
20 25 30
<210> 725
<211> 90
<212> DNA
<213> 弯曲乳杆菌 L442
<400> 725
gcgtatccgg gcaacggcgt gcattgcggc aaatatagct gcaccgtgga taaacagacc 60
gcgattggca acattggcaa caacgcggcg 90
<210> 726
<211> 43
<212> PRT
<213> 分歧食肉杆菌
<400> 726
Thr Lys Tyr Tyr Gly Asn Gly Val Tyr Cys Asn Ser Lys Lys Cys Trp
1 5 10 15
Val Asp Trp Gly Thr Ala Gln Gly Cys Ile Asp Val Val Ile Gly Gln
20 25 30
Leu Gly Gly Gly Ile Pro Gly Lys Gly Lys Cys
35 40
<210> 727
<211> 129
<212> DNA
<213> 分歧食肉杆菌
<400> 727
accaaatatt atggcaacgg cgtgtattgc aacagcaaaa aatgctgggt ggattggggc 60
accgcgcagg gctgcattga tgtggtgatt ggccagctgg gcggcggcat tccgggcaaa 120
ggcaaatgc 129
<210> 728
<211> 24
<212> PRT
<213> 微双孢菌(菌株107891
<400> 728
Val Thr Ser Trp Ser Leu Cys Thr Pro Gly Cys Thr Ser Pro Gly Gly
1 5 10 15
Gly Ser Asn Cys Ser Phe Cys Cys
20
<210> 729
<211> 72
<212> DNA
<213> 微双孢菌(菌株107891
<400> 729
gtgaccagct ggagcctgtg caccccgggc tgcaccagcc cgggcggcgg cagcaactgc 60
agcttttgct gc 72
<210> 730
<211> 62
<212> PRT
<213> 肠球菌
<400> 730
Asn Arg Trp Tyr Cys Asn Ser Ala Ala Gly Gly Val Gly Gly Ala Ala
1 5 10 15
Val Cys Gly Leu Ala Gly Tyr Val Gly Glu Ala Lys Glu Asn Ile Ala
20 25 30
Gly Glu Val Arg Lys Gly Trp Gly Met Ala Gly Gly Phe Thr His Asn
35 40 45
Lys Ala Cys Lys Ser Phe Pro Gly Ser Gly Trp Ala Ser Gly
50 55 60
<210> 731
<211> 186
<212> DNA
<213> 肠球菌
<400> 731
aaccgctggt attgcaacag cgcggcgggc ggcgtgggcg gcgcggcggt gtgcggcctg 60
gcgggctatg tgggcgaagc gaaagaaaac attgcgggcg aagtgcgcaa aggctggggc 120
atggcgggcg gctttaccca taacaaagcg tgcaaaagct ttccgggcag cggctgggcg 180
agcggc 186
<210> 732
<211> 39
<212> PRT
<213> 屎肠球菌
<400> 732
Thr Thr Lys Asn Tyr Gly Asn Gly Val Cys Asn Ser Val Asn Trp Cys
1 5 10 15
Gln Cys Gly Asn Val Trp Ala Ser Cys Asn Leu Ala Thr Gly Cys Ala
20 25 30
Ala Trp Leu Cys Lys Leu Ala
35
<210> 733
<211> 117
<212> DNA
<213> 屎肠球菌
<400> 733
accaccaaaa actatggcaa cggcgtgtgc aacagcgtga actggtgcca gtgcggcaac 60
gtgtgggcga gctgcaacct ggcgaccggc tgcgcggcgt ggctgtgcaa actggcg 117
<210> 734
<211> 30
<212> PRT
<213> 多粘类芽孢杆菌
<400> 734
Ala Ser Ile Ile Lys Thr Thr Ile Lys Val Ser Lys Ala Val Cys Lys
1 5 10 15
Thr Leu Thr Cys Ile Cys Thr Gly Ser Cys Ser Asn Cys Lys
20 25 30
<210> 735
<211> 90
<212> DNA
<213> 多粘类芽孢杆菌
<400> 735
gcgagcatta ttaaaaccac cattaaagtg agcaaagcgg tgtgcaaaac cctgacctgc 60
atttgcaccg gcagctgcag caactgcaaa 90
<210> 736
<211> 31
<212> PRT
<213> 表皮葡萄球菌
<400> 736
Ser Ala Ser Ile Val Lys Thr Thr Ile Lys Ala Ser Lys Lys Leu Cys
1 5 10 15
Arg Gly Phe Thr Leu Thr Cys Gly Cys His Phe Thr Gly Lys Lys
20 25 30
<210> 737
<211> 93
<212> DNA
<213> 表皮葡萄球菌
<400> 737
agcgcgagca ttgtgaaaac caccattaaa gcgagcaaaa aactgtgccg cggctttacc 60
ctgacctgcg gctgccattt taccggcaaa aaa 93
<210> 738
<211> 58
<212> PRT
<213> 屎肠球菌
<400> 738
Met Glu Lys Leu Thr Val Lys Glu Met Ser Gln Val Val Gly Gly Lys
1 5 10 15
Tyr Tyr Gly Asn Gly Val Ser Cys Asn Lys Lys Gly Cys Ser Val Asp
20 25 30
Trp Gly Lys Ala Ile Gly Ile Ile Gly Asn Asn Ala Ala Ala Asn Leu
35 40 45
Thr Thr Gly Gly Lys Ala Gly Trp Lys Gly
50 55
<210> 739
<211> 178
<212> DNA
<213> 屎肠球菌
<400> 739
atggaaaaat taactgtgaa agaaatgtcg caagtagttg gcggaaagta ctatggtaac 60
ggagtatcat gtaataaaaa gggatgtagt gttgattggg gaaaagctat tggtattatt 120
ggaaataatg ctgctgctaa tttaactact ggcggaaaag cagggtggaa aggttaac 178
<210> 740
<211> 39
<212> PRT
<213> 多粘类芽孢杆菌
<400> 740
Ala Thr Tyr Tyr Gly Asn Gly Leu Tyr Cys Asn Lys Gln Lys His Tyr
1 5 10 15
Thr Trp Val Asp Trp Asn Lys Ala Ser Arg Glu Ile Gly Lys Ile Thr
20 25 30
Val Asn Gly Trp Val Gln His
35
<210> 741
<211> 93
<212> DNA
<213> 多粘类芽孢杆菌
<400> 741
agcgcgagca ttgtgaaaac caccattaaa gcgagcaaaa aactgtgccg cggctttacc 60
ctgacctgcg gctgccattt taccggcaaa aaa 93
<210> 742
<211> 35
<212> PRT
<213> 环状芽胞杆菌
<400> 742
Val Asn Tyr Gly Asn Gly Val Ser Cys Ser Lys Thr Lys Cys Ser Val
1 5 10 15
Asn Trp Gly Ile Ile Thr His Gln Ala Phe Arg Val Thr Ser Gly Val
20 25 30
Ala Ser Gly
35
<210> 743
<211> 105
<212> DNA
<213> 环状芽胞杆菌
<400> 743
gtgaactatg gcaacggcgt gagctgcagc aaaaccaaat gcagcgtgaa ctggggcatt 60
attacccatc aggcgtttcg cgtgaccagc ggcgtggcga gcggc 105
<210> 744
<211> 30
<212> PRT
<213> 多粘类芽孢杆菌
<400> 744
Phe Val Tyr Gly Asn Gly Val Thr Ser Ile Leu Val Gln Ala Gln Phe
1 5 10 15
Leu Val Asn Gly Gln Arg Arg Phe Phe Tyr Thr Pro Asp Lys
20 25 30
<210> 745
<211> 90
<212> DNA
<213> 多粘类芽孢杆菌
<400> 745
tttgtgtatg gcaacggcgt gaccagcatt ctggtgcagg cgcagtttct ggtgaacggc 60
cagcgccgct ttttttatac cccggataaa 90
<210> 746
<211> 13
<212> PRT
<213> 鼠李糖乳杆菌
<400> 746
Ala Val Pro Ala Val Arg Lys Thr Asn Glu Thr Leu Asp
1 5 10
<210> 747
<211> 39
<212> DNA
<213> 鼠李糖乳杆菌
<400> 747
gcggtgccgg cggtgcgcaa aaccaacgaa accctggat 39
<210> 748
<211> 60
<212> PRT
<213> 地衣芽孢杆菌
<400> 748
Met Lys Asn Ser Ala Ala Arg Glu Ala Phe Lys Gly Ala Asn His Pro
1 5 10 15
Ala Gly Met Val Ser Glu Glu Glu Leu Lys Ala Leu Val Gly Gly Asn
20 25 30
Asp Val Asn Pro Glu Thr Thr Pro Ala Thr Thr Ser Ser Trp Thr Cys
35 40 45
Ile Thr Ala Gly Val Thr Val Ser Ala Ser Leu Cys
50 55 60
<210> 749
<211> 180
<212> DNA
<213> 地衣芽孢杆菌
<400> 749
atgaaaaaca gcgcggcgcg cgaagcgttt aaaggcgcga accatccggc gggcatggtg 60
agcgaagaag aactgaaagc gctggtgggc ggcaacgatg tgaacccgga aaccaccccg 120
gcgaccacca gcagctggac ctgcattacc gcgggcgtga ccgtgagcgc gagcctgtgc 180
<210> 750
<211> 689
<212> PRT
<213> 绿脓假单胞菌
<400> 750
Met Ala Val Asn Asp Tyr Glu Pro Gly Ser Met Val Ile Thr His Val
1 5 10 15
Gln Gly Gly Gly Arg Asp Ile Ile Gln Tyr Ile Pro Ala Arg Ser Ser
20 25 30
Tyr Gly Thr Pro Pro Phe Val Pro Pro Gly Pro Ser Pro Tyr Val Gly
35 40 45
Thr Gly Met Gln Glu Tyr Arg Lys Leu Arg Ser Thr Leu Asp Lys Ser
50 55 60
His Ser Glu Leu Lys Lys Asn Leu Lys Asn Glu Thr Leu Lys Glu Val
65 70 75 80
Asp Glu Leu Lys Ser Glu Ala Gly Leu Pro Gly Lys Ala Val Ser Ala
85 90 95
Asn Asp Ile Arg Asp Glu Lys Ser Ile Val Asp Ala Leu Met Asp Ala
100 105 110
Lys Ala Lys Ser Leu Lys Ala Ile Glu Asp Arg Pro Ala Asn Leu Tyr
115 120 125
Thr Ala Ser Asp Phe Pro Gln Lys Ser Glu Ser Met Tyr Gln Ser Gln
130 135 140
Leu Leu Ala Ser Arg Lys Phe Tyr Gly Glu Phe Leu Asp Arg His Met
145 150 155 160
Ser Glu Leu Ala Lys Ala Tyr Ser Ala Asp Ile Tyr Lys Ala Gln Ile
165 170 175
Ala Ile Leu Lys Gln Thr Ser Gln Glu Leu Glu Asn Lys Ala Arg Ser
180 185 190
Leu Glu Ala Glu Ala Gln Arg Ala Ala Ala Glu Val Glu Ala Asp Tyr
195 200 205
Lys Ala Arg Lys Ala Asn Val Glu Lys Lys Val Gln Ser Glu Leu Asp
210 215 220
Gln Ala Gly Asn Ala Leu Pro Gln Leu Thr Asn Pro Thr Pro Glu Gln
225 230 235 240
Trp Leu Glu Arg Ala Thr Gln Leu Val Thr Gln Ala Ile Ala Asn Lys
245 250 255
Lys Lys Leu Gln Thr Ala Asn Asn Ala Leu Ile Ala Lys Ala Pro Asn
260 265 270
Ala Leu Glu Lys Gln Lys Ala Thr Tyr Asn Ala Asp Leu Leu Val Asp
275 280 285
Glu Ile Ala Ser Leu Gln Ala Arg Leu Asp Lys Leu Asn Ala Glu Thr
290 295 300
Ala Arg Arg Lys Glu Ile Ala Arg Gln Ala Ala Ile Arg Ala Ala Asn
305 310 315 320
Thr Tyr Ala Met Pro Ala Asn Gly Ser Val Val Ala Thr Ala Ala Gly
325 330 335
Arg Gly Leu Ile Gln Val Ala Gln Gly Ala Ala Ser Leu Ala Gln Ala
340 345 350
Ile Ser Asp Ala Ile Ala Val Leu Gly Arg Val Leu Ala Ser Ala Pro
355 360 365
Ser Val Met Ala Val Gly Phe Ala Ser Leu Thr Tyr Ser Ser Arg Thr
370 375 380
Ala Glu Gln Trp Gln Asp Gln Thr Pro Asp Ser Val Arg Tyr Ala Leu
385 390 395 400
Gly Met Asp Ala Ala Lys Leu Gly Leu Pro Pro Ser Val Asn Leu Asn
405 410 415
Ala Val Ala Lys Ala Ser Gly Thr Val Asp Leu Pro Met Arg Leu Thr
420 425 430
Asn Glu Ala Arg Gly Asn Thr Thr Thr Leu Ser Val Val Ser Thr Asp
435 440 445
Gly Val Ser Val Pro Lys Ala Val Pro Val Arg Met Ala Ala Tyr Asn
450 455 460
Ala Thr Thr Gly Leu Tyr Glu Val Thr Val Pro Ser Thr Thr Ala Glu
465 470 475 480
Ala Pro Pro Leu Ile Leu Thr Trp Thr Pro Ala Ser Pro Pro Gly Asn
485 490 495
Gln Asn Pro Ser Ser Thr Thr Pro Val Val Pro Lys Pro Val Pro Val
500 505 510
Tyr Glu Gly Ala Thr Leu Thr Pro Val Lys Ala Thr Pro Glu Thr Tyr
515 520 525
Pro Gly Val Ile Thr Leu Pro Glu Asp Leu Ile Ile Gly Phe Pro Ala
530 535 540
Asp Ser Gly Ile Lys Pro Ile Tyr Val Met Phe Arg Asp Pro Arg Asp
545 550 555 560
Val Pro Gly Ala Ala Thr Gly Lys Gly Gln Pro Val Ser Gly Asn Trp
565 570 575
Leu Gly Ala Ala Ser Gln Gly Glu Gly Ala Pro Ile Pro Ser Gln Ile
580 585 590
Ala Asp Lys Leu Arg Gly Lys Thr Phe Lys Asn Trp Arg Asp Phe Arg
595 600 605
Glu Gln Phe Trp Ile Ala Val Ala Asn Asp Pro Glu Leu Ser Lys Gln
610 615 620
Phe Asn Pro Gly Ser Leu Ala Val Met Arg Asp Gly Gly Ala Pro Tyr
625 630 635 640
Val Arg Glu Ser Glu Gln Ala Gly Gly Arg Ile Lys Ile Glu Ile His
645 650 655
His Lys Val Arg Ile Ala Asp Gly Gly Gly Val Tyr Asn Met Gly Asn
660 665 670
Leu Val Ala Val Thr Pro Lys Arg His Ile Glu Ile His Lys Gly Gly
675 680 685
Lys
<210> 751
<211> 2070
<212> DNA
<213> 绿脓假单胞菌
<400> 751
atggctgtca atgattacga acctggttcg atggttatta cacatgtgca gggtggtggg 60
cgtgacataa tccagtatat tcctgctcga tcaagctacg gtactccacc atttgtccca 120
ccaggaccaa gtccgtatgt cggtactgga atgcaggagt acaggaagct aagaagtacg 180
cttgataagt cccattcaga actcaagaaa aacctgaaaa atgaaaccct gaaggaggtt 240
gatgaactca agagtgaagc ggggttgcca ggtaaagcgg tcagtgccaa tgacatccgc 300
gatgaaaaga gtatcgttga tgcactcatg gatgccaaag caaaatcgct aaaggccatt 360
gaggatcgcc cggccaatct ttatacggct tcagactttc ctcagaagtc agagtcgatg 420
taccagagtc agttgctggc cagccgaaaa ttctatggag agttcctgga tcgccatatg 480
agtgagctgg ccaaagcgta cagcgccgat atctataagg cgcaaatcgc tatcttgaaa 540
caaacgtctc aagagctgga gaataaagcc cggtcattgg aagcagaagc ccagcgagcc 600
gctgctgagg tggaggcgga ctacaaggcc aggaaggcaa atgtcgagaa aaaagtgcag 660
tccgagcttg accaggctgg gaatgctttg cctcaactga ccaatccaac gccagagcag 720
tggcttgaac gcgctactca actggttacg caggcgatcg ccaataagaa gaaattgcag 780
actgcaaaca atgccttgat tgccaaggca cccaatgcac tggagaaaca aaaggcaacc 840
tacaacgccg atctcctagt ggatgaaatc gccagcctgc aagcacggct ggacaagctg 900
aacgccgaaa cggcaaggcg caaggaaatc gctcgtcaag cggcgatcag ggctgccaat 960
acttatgcca tgccagccaa tggcagcgtt gtcgccaccg ccgcaggccg gggtctgatc 1020
caggtcgcac aaggcgccgc atcccttgct caagcgatct ccgatgcgat tgccgtcctg 1080
ggccgggtcc tggcttcagc accctcggtg atggccgtgg gctttgccag tctgacctac 1140
tcctcccgga ctgccgagca atggcaggac caaacgcccg atagcgttcg ttacgccctg 1200
ggcatggatg ccgctaaatt ggggcttccc ccaagcgtaa acctgaacgc ggttgcaaaa 1260
gccagcggta ccgtcgatct gccgatgcgc ctgaccaacg aggcacgagg caacacgacg 1320
accctttcgg tggtcagcac cgatggtgtg agcgttccga aagccgttcc ggtccggatg 1380
gcggcctaca atgccacgac aggcctgtac gaggttacgg ttccctctac gaccgcagaa 1440
gcgccgccac tgatcctgac ctggacgccg gcgagtcctc caggaaacca gaacccttcg 1500
agtaccactc cggtcgtacc gaagccggtg ccggtatatg agggagcgac ccttacaccg 1560
gtgaaggcta ccccggaaac ctatcctggg gtgattacac taccggaaga cctgatcatc 1620
ggcttcccgg ccgactcggg gatcaagccg atctatgtga tgttcaggga tccgcgggat 1680
gtacctggtg ctgcgactgg caagggacag cccgtcagcg gtaattggct cggcgccgcc 1740
tctcaaggtg agggggctcc aattccaagc cagattgcgg ataaactacg tggtaagaca 1800
ttcaaaaact ggcgggactt tcgggaacaa ttctggatag ctgtggctaa tgatcctgag 1860
ttaagtaaac agtttaatcc tggtagttta gctgtaatga gagatggagg ggctccttat 1920
gtcagagagt cagaacaggc tggcgggaga ataaagatcg aaatccacca caaggttcga 1980
atagcagatg gaggcggcgt ttacaatatg gggaaccttg ttgcagtaac gccaaaacgt 2040
catatagaaa tccacaaggg agggaagtga 2070
<210> 752
<211> 36
<212> PRT
<213> 植物乳杆菌
<400> 752
Lys Tyr Tyr Gly Asn Gly Leu Ser Cys Ser Lys Lys Gly Cys Thr Val
1 5 10 15
Asn Trp Gly Gln Ala Phe Ser Cys Gly Val Asn Arg Val Ala Thr Ala
20 25 30
Gly His Gly Lys
35
<210> 753
<211> 108
<212> DNA
<213> 植物乳杆菌
<400> 753
aaatattatg gcaacggcct gagctgcagc aaaaaaggct gcaccgtgaa ctggggccag 60
gcgtttagct gcggcgtgaa ccgcgtggcg accgcgggcc atggcaaa 108
<210> 754
<211> 30
<212> PRT
<213> 乳酸乳球菌
<400> 754
Met Lys Thr Ile Leu Arg Phe Val Ala Gly Tyr Asp Ile Ala Ser His
1 5 10 15
Lys Lys Lys Thr Gly Gly Tyr Pro Trp Glu Arg Gly Lys Ala
20 25 30
<210> 755
<211> 93
<212> DNA
<213> 乳酸乳球菌
<400> 755
atgaaaacaa tcctacgttt tgttgctggc tacgatattg ctagtcataa aaagaaaact 60
ggcggctatc catgggaacg tggaaaagct taa 93
<210> 756
<211> 24
<212> PRT
<213> 嗜酸乳杆菌
<400> 756
Gly Asn Pro Lys Val Ala His Cys Ala Ser Gln Ile Gly Arg Ser Thr
1 5 10 15
Ala Trp Gly Ala Val Ser Gly Ala
20
<210> 757
<211> 72
<212> DNA
<213> 嗜酸乳杆菌
<400> 757
ggcaacccga aagtggcgca ttgcgcgagc cagattggcc gcagcaccgc gtggggcgcg 60
gtgagcggcg cg 72
<210> 758
<211> 53
<212> PRT
<213> 唾液乳杆菌cp400
<400> 758
Met Phe Phe Asn Phe Met Lys Lys Val Asp Val Lys Lys Asn Phe Gly
1 5 10 15
Tyr Lys Glu Val Ser Arg Lys Asp Leu Ala Lys Val Asn Gly Gly Lys
20 25 30
Arg Lys Lys His Arg Cys Arg Val Tyr Asn Asn Gly Met Pro Thr Gly
35 40 45
Met Tyr Arg Trp Cys
50
<210> 759
<211> 162
<212> DNA
<213> 唾液乳杆菌cp400
<400> 759
atgtttttta attttatgaa aaaagtagat gtgaagaaga attttggata taaagaagtt 60
tctagaaaag atctagctaa agtaaatggt ggaaagagaa agaaacatcg ttgcagagtt 120
tataataatg gaatgcctac aggaatgtat cgttggtgct aa 162
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