阅读说明：本技术 一种木瓜总三萜的制药用途 (Pharmaceutical application of total triterpene of pawpaw ) 是由 贺海波 石孟琼 廖勇 宋利华 张媛媛 何毓敏 卫巧 程凡 邹坤 张继红 于 2020-07-16 设计创作，主要内容包括：本发明公开了一种木瓜总三萜的制药用途。具体包括木瓜总三萜在制备促进UVB损伤的人皮肤成纤维细胞HDF细胞增殖、细胞迁移、抑制细胞凋亡的药物上的应用。以及制备促进UVB损伤的人脐静脉内皮细胞HUVECs细胞增殖、细胞迁移、抑制细胞凋亡的药物上的应用。以及制备治疗术后切割损伤伤口愈合的药物上的应用。以及制备治疗受损皮肤再生修复的药物上的应用。具有显著的治疗效果。(The invention discloses a pharmaceutical application of total triterpene of pawpaw. The application of the total triterpene of the pawpaw in preparing the medicament for promoting the proliferation and cell migration of human skin fibroblast HDF cells damaged by UVB and inhibiting apoptosis is concretely included. And the application of the HUVECs in preparing medicaments for promoting the cell proliferation and cell migration of human umbilical vein endothelial cells damaged by UVB and inhibiting cell apoptosis. And the application in preparing the medicine for healing the postoperative cutting injury wound. And the application in preparing the medicine for treating the regeneration and repair of the damaged skin. Has obvious curative effect.)

1. The application of the total triterpene of the pawpaw in preparing the medicine for promoting the proliferation and cell migration of human skin fibroblast HDF cells damaged by UVB and inhibiting apoptosis.

2. Application of total triterpene of fructus Chaenomelis in preparing medicine for promoting cell proliferation and cell migration of HUVECs of human umbilical vein endothelial cell damaged by UVB and inhibiting apoptosis is provided.

3. Application of total triterpene of fructus Chaenomelis in preparing medicine for healing wound after operation.

4. Application of total triterpene of fructus Chaenomelis in preparing medicine for treating damaged skin regeneration and repair is provided.

Technical Field

The invention belongs to the technical field of skin repair, and particularly relates to a preparation process and application of pawpaw total triterpene hydrogel.

Background

The skin is the largest organ of the human body, has a plurality of important functions of metabolism, absorption, protection, thermoregulation, secretion, sensation and the like, and is easy to cause damage when being stimulated by external chemical, physical and other factors. The healing process of skin after acute trauma is very easy to cause scar and wound contraction, and normal skin function, beauty and psychology are affected, so that the regeneration and function repair of the skin after injury become focus of attention of clinicians. Particularly, a patient with full-thickness skin defect can heal the wound mainly through the migration of the skin epithelial cells and the regeneration of the stem cells of the residual skin appendages, if the skin defect area of the patient is too large or the full-thickness skin is damaged/defected, the skin cannot be completely regenerated, the wound surface forms scars, the healing is delayed or not healed, the patient suffers from serious dysfunction, and the patient suffers great pain. Therefore, it is important to promote the healing of the skin wound and reduce the scar formation.

At present, methods for healing skin wounds mainly include gene engineering application, bioengineering material application, nanotechnology application, nutrition adjuvant therapy and the like, but have the defects of large individual difference of curative effect, high price, poor patient compliance, easy scar generation after healing and the like, the traditional Chinese medicine has a long history of medical record for treating skin, is rich in experience, creates a plurality of different dosage forms, and has good curative effect in treating skin diseases by extracting traditional Chinese medicines along with scientific development in recent years.

Fructus Chaenomelis is of Rosaceae plant Chaenomelis speciosa (sweet) nakaiChaenomeles speciosa(Sweet) dried nearly mature fruit of Nakai, also known as Chaenomeles speciosa Nakai, Pyrus callorhinus Linne, Chaenomeles speciosa (Sweet) Nakai, etc.). Pawpaw is called 'Baiyi fruit' and is a very important ornamental and edible plant, and is listed as one of the first medical and edible foods identified by the ministry of health in 2003. It is used as an edible fruit and is also used in the field of cosmetic and health care products. The total triterpene of fructus Chaenomelis is an important active ingredient in Chaenomeles speciosa nakai, and its main ingredients include oleanolic acid, ursolic acid, betulinic acid, 3-O-acetyl ursolic acid, 3-O-acetyl palygosamine acid, maslinic acid, tormentic acid and specificosaperoxide. The content of the total triterpene of the pawpaw in the fresh fruit is more than 0.32 percent, and the total content of the enriched total triterpene of the pawpaw is 90.02 percent. Specific methods are described in our issued patents: a Chaenomeles speciosa extract, its extraction method and its application (patent No. ZL201510660595.8) are described in detail.

Disclosure of Invention

The inventor of the invention has conducted extensive and intensive research, and finds that the total triterpene of pawpaw extracted from Tujia medicine chaenomeles speciosa can effectively promote the proliferation and migration of human dermal fibroblasts and vascular endothelial cells, has good effects of promoting skin repair and wound healing and reducing scar generation on damaged skin wounds, and has no obvious toxic or side effect. At present, reports related to the preparation method of the total triterpene of the pawpaw prepared by the invention and the promotion of the wound healing of the damaged skin are not found.

The invention aims to provide a pharmaceutical application of total triterpenes in pawpaw. The specific pharmaceutical application is as follows:

the application of the total triterpene of the pawpaw in preparing the medicine for promoting the proliferation and cell migration of human skin fibroblast HDF cells damaged by UVB and inhibiting apoptosis.

Application of total triterpene of fructus Chaenomelis in preparing medicine for promoting cell proliferation and cell migration of HUVECs of human umbilical vein endothelial cell damaged by UVB and inhibiting apoptosis is provided.

Application of total triterpene of fructus Chaenomelis in preparing medicine for healing wound after operation.

Application of total triterpene of fructus Chaenomelis in preparing medicine for treating damaged skin regeneration and repair is provided.

The total triterpenoids of the wrinkled pAN _ SNer majoram prepared by the preparation method have good biocompatibility, nontoxicity, resistibility and good biodegradability, can effectively avoid the first pass effect of a medicine liver, can realize directional administration and have lasting effect, thereby having better application and development prospects.

The invention has the advantages of

The pawpaw total triterpenes prepared by the invention have better effects of promoting proliferation, inhibiting apoptosis and promoting cell migration in human dermal fibroblasts and vascular endothelial cell injury models; the Chinese medicinal composition has good treatment effect on rat skin injury models and skin trauma patients caused by surgical cutting, and can remarkably promote the healing of skin wound surfaces, shorten the healing time of wounds, promote the regeneration and repair of damaged skin and reduce the generation of scars.