阅读说明：本技术 冷蒿水提物其制备方法和应用 (Artemisia frigida water extract and preparation method and application thereof ) 是由 李秀梅 杨培龙 石冬冬 闻治国 王亚冬 杨娟 于 2020-07-01 设计创作，主要内容包括：本发明涉及农业生物技术领域,具体涉及冷蒿水提物及其制备方法和应用。本发明用超声提取法,以蒸馏水作为提取溶剂,获得具有很好的抗炎作用冷蒿水提物。(The invention relates to the technical field of agricultural biology, in particular to a artemisia selengensis water extract and a preparation method and application thereof. The invention uses ultrasonic extraction method and uses distilled water as extraction solvent to obtain the artemisia selengensis water extract with good anti-inflammatory effect.)

1. The artemisia selengensis water extract is characterized by being prepared by the following steps,

preparing a cold wormwood water extract, crushing cold wormwood, performing ultrasonic water extraction, and performing ultrasonic conditions: ultrasonic power of 500W, temperature of 50 ℃, time of 30min, and obtaining the water extract.

2. The cold wormwood aqueous extract according to claim 1, wherein the ratio of water to cold wormwood powder is 40:1 mL/g.

3. The preparation method of the artemisia selengensis aqueous extract is characterized by comprising the following steps of:

crushing the artemisia selengensis, carrying out ultrasonic water extraction, wherein the ultrasonic conditions are as follows: ultrasonic power is 500W, temperature is 50 ℃, time is 30min, and the obtained water extract is evaporated and concentrated.

4. A method of preparing a cold wormwood extract according to claim 3, wherein the ratio of water to cold fleshy powder is 40:1 mL/g.

5. Use of an aqueous extract of Artemisia frigida of claim 1 for the preparation of a medicament for inhibiting inflammation.

Technical Field

The invention relates to the technical field of agricultural biology, in particular to a artemisia selengensis water extract and a preparation method and application thereof.

Background

Inflammation, a defense measure against infection or tissue damage, can occur in various tissues and organs of the human body, such as hepatitis, gastritis, pneumonia, etc. Among them, bacterial infection or viral infection, burns and cuts, strong acid and alkali corrosion, and immune system disorders of the human body can cause inflammation of the body, and these factors are called inflammatory factors.

Artemisia frigida Willd is a perennial herb of Artemisia (Artemisia) of Compositae (Compositae). The total flavone extract of herba Artemisiae Scopariae has obvious antiinflammatory effect. However, the effective active ingredients of the anti-inflammatory action of the artemisia frigida and the action mechanism thereof are not clear.

Disclosure of Invention

The invention aims to provide an artemisia selengensis aqueous extract.

The invention also aims to provide a preparation method of the artemisia selengensis water extract.

The invention also aims to provide the application of the artemisia selengensis water extract.

The anti-inflammatory fraction in the artemisia selengensis water extract is prepared by the following steps,

crushing the artemisia selengensis, carrying out ultrasonic water extraction, wherein the ultrasonic conditions are as follows: ultrasonic power is 500W, temperature is 50 ℃, time is 30min, and the obtained water extract is evaporated and concentrated for standby.

The invention relates to a cold wormwood water extract, wherein the ratio of water to cold wormwood powder is 40:1 mL/g.

The preparation method of the artemisia selengensis aqueous extract comprises the following steps:

crushing the artemisia selengensis, carrying out ultrasonic water extraction, wherein the ultrasonic conditions are as follows: ultrasonic power is 500W, temperature is 50 ℃, time is 30min, and the obtained water extract is evaporated and concentrated for standby.

The invention also provides application of the artemisia selengensis water extract in preparing a medicament for inhibiting inflammation.

The invention uses ultrasonic extraction method and uses distilled water as extraction solvent to obtain the artemisia selengensis water extract, thereby ensuring that the artemisia selengensis water extract has good anti-inflammatory effect.

Drawings

FIG. 1 shows the effect of aqueous herbal extracts on cell viability;

FIG. 2 shows the effect of aqueous herbal extracts on cell viability;

FIG. 3 shows the effect of aqueous herbal extracts on cell viability;

FIG. 4 shows the effect of aqueous herbal extracts on cell viability;

FIG. 5 shows that ELISA kit detects the content of nitric oxide in the supernatant of RAW264.7 cells;

FIG. 6 shows that ELISA kit detects the content of interleukin-6 in the supernatant of RAW264.7 cells;

FIG. 7 shows that ELISA kit detects the content of interleukin-1 beta in the supernatant of RAW264.7 cells;

FIG. 8 shows that the ELISA kit detects the content of TNF-alpha in the supernatant of RAW264.7 cells.

Detailed Description