阅读说明：本技术 一种动物皮活性肽的制备方法及其应用 (Preparation method and application of animal skin active peptide ) 是由 汪建明 闫可心 李倩倩 于 2019-03-29 设计创作，主要内容包括：本发明涉及一种动物皮活性肽制备方法及其应用,所述制备方法具体为：将动物皮洗净分切后经高压蒸煮、绞碎、去毛、酶解、灭酶、过滤后进行浓缩制得。制得的动物活性肽可制成各种护肤品,应用于改善皮肤干燥缺水、提亮肤色、缓解皮肤老化。本发明制备方法简单可行、成本低、工艺稳定、得率高,得到的产品纯度和质量高。(The invention relates to a preparation method and application of animal skin active peptide, wherein the preparation method specifically comprises the following steps: cleaning and cutting animal skin, steaming under high pressure, mincing, unhairing, performing enzymolysis, inactivating enzyme, filtering, and concentrating. The obtained animal active peptide can be made into various skin care products, and can be used for improving skin dryness and water deficiency, brightening skin color, and relieving skin aging. The preparation method is simple and feasible, low in cost, stable in process, high in yield, and high in purity and quality of the obtained product.)

1. A process for preparing the active peptide of animal skin includes such steps as washing and cutting the animal skin, high-pressure steaming, mincing, removing hair, enzymolyzing, deactivating enzyme, filtering and concentrating.

2. The method for preparing active peptide from animal skin as claimed in claim 1, wherein the animal skin is selected from the group consisting of: horse hide, camel hide, and deer hide.

3. The method for preparing active peptide from animal skin according to claim 1, wherein the autoclaving conditions are as follows: mixing the block animal skin with water at a ratio of 1: 3-1: 6, stirring, placing into autoclave, and steaming at 121 deg.C for 20-50 min.

4. The method for preparing active peptide of animal skin as claimed in claim 1, wherein said dehairing mode is: plate and frame filtration or centrifugal filtration.

5. The method for preparing active peptide on animal skin according to claim 1, wherein the conditions of enzymolysis are as follows: performing enzymolysis with protease at 35-40 deg.C for 5-9 hr under pH of 4-10.5.

6. The method of claim 5, wherein the protease is one or more of an acid protease, a neutral protease and an alkaline protease.

7. The process of claim 1, wherein the filtration conditions are as follows: adopting active carbon for adsorption and then filtering.

8. The method for preparing the animal skin active peptide according to claim 1, wherein the animal skin active peptide can be prepared into various skin care products for improving dry skin and water deficiency, brightening skin color and relieving skin aging.

Technical Field

The invention belongs to the technical field of daily chemicals, and particularly relates to a preparation method of an animal skin active peptide.

Background

China is a large animal husbandry country, and by-products produced by slaughtering livestock and poultry cannot be ignored along with the development of livestock and poultry breeding in China. The animal skin is a wide resource, cheap and easy to obtain. Horse skin, camel skin and deer skin are rich in various proteins, and can be used as source of active peptide. However, the animal skins are mostly used as raw materials for paper making and leather in the Chinese market, the development and utilization degree is not high, and the animal skin leftover materials are used as peptide sources, so that waste can be changed into valuable.

Chinese patent CN 108796017a discloses bovine bone peptide and an enzymatic extraction method thereof, which discloses bovine bone peptide and an enzymatic extraction method thereof, and is characterized in that the deep extraction of bovine bone is realized by adopting an enzymatic extraction technology, collagen in bovine bone is fully released and converted into bovine bone peptide, and the extraction effect is greatly improved. Patent CN 107353322A relates to a yak skin peptide product with antioxidant effect and its preparation method, and the patent adopts leaching membrane filtration to extract yak skin peptide. Patent CN 104479995a discloses a sheepskin collagen extraction system and an extraction method, which comprises a reaction device and a spray drying device connected in sequence. The extraction method comprises the steps of pretreatment, cleaning, crushing, enzymolysis, enzyme deactivation, filtration, ultrafiltration, ion exchange, spray drying and the like.

At present, horse skin, camel skin and deer skin are few products as peptide sources, fish skin peptide is taken as a main variety, and a small amount of sheep skin collagen is added to prepare camel skin peptide by an extraction method. The sources and the preparation methods of the peptides are different, the method for preparing the animal skin active peptide by the enzymolysis method is simple and feasible, the cost is low, the process is stable, the yield is high, and the purity and the quality of the obtained product are high. In view of the current situation, the applicant invents a preparation method of animal skin active peptide and researches the application of the animal skin active peptide through systematic research. The animal skin active peptide can be made into various skin care products, and can be used for improving skin dryness and water deficiency, brightening skin color, and relieving skin aging.

Disclosure of Invention

The invention overcomes the defects of the prior art and products, invents a simple and feasible preparation method of the animal skin active peptide with low cost and stable process, and adopts the animal skin active peptide to prepare the skin care product.

The purpose of the invention is realized by the following technical scheme:

a preparation method of animal skin active peptide is characterized by comprising the following steps:

(1) high-temperature cooking: mixing the block horse, camel or deer skin with water at a ratio of 1: 3-1: 6, stirring, placing into autoclave, and steaming at 121 deg.C for 20-50 min.

(2) And (3) mincing: mincing the softened animal skin after high-pressure cooking by adopting a meat mincer. Mincing every 10S for 6-8 times at about 5S.

(3) Unhairing: and (4) removing hair by plate and frame filtration or centrifugal filtration after mincing.

(4) Enzymolysis: performing enzymolysis with protease at 35-40 deg.C for 5-9 hr under pH of 4-10.5. The protease includes acid protease, neutral protease and alkaline protease.

(5) Enzyme deactivation: inactivating enzyme in autoclave at 121 deg.C for 20-30 min.

(6) And (3) filtering: adopting active carbon for adsorption and then filtering.

(7) Concentration: vacuum rotary evaporation is carried out under the conditions that the heating temperature is 50-60 ℃, the temperature of a cooling medium is 20-25 ℃ and the pressure is 2-3 KPa.

The application of the skin care product containing the animal skin active peptide is characterized in that the skin care product is applied to improve the dryness and water shortage of the skin, brighten the skin color and relieve the skin aging.

Further, the cosmetic may be in the form of a toner, lotion, cream, essence, pack, or spray.

Further, the cosmetic may further comprise cosmetically acceptable adjuvants including, but not limited to, preservatives, antioxidants, humectants, thickeners, chelating agents, stabilizers, and emulsifiers.

The invention has the advantages and positive effects that:

1. the animal skin active peptide is prepared by an enzymolysis method, the method is simple and feasible, the cost is low, the process is stable, the yield is high, and the purity and the quality of the obtained product are high. Horse, camel or deerskin is wide in resource, cheap and easy to obtain, and horse skin is rich in multiple proteins.

2. The animal skin active peptide is obtained by carrying out enzymolysis on animal skin, and then is mixed with other raw materials (preservative, antioxidant, humectant, thickener, chelating agent, stabilizer and emulsifier), so that the animal skin active peptide can improve the dryness and water shortage of skin, brighten the skin color and relieve the skin aging.

Detailed Description

The present invention will be further described by the following specific examples, which are illustrative only and not limiting, and the scope of the present invention is not limited thereby.