阅读说明：本技术 一种飞行时间核酸质谱激光时序算法及控制方法 (Time-of-flight nucleic acid mass spectrum laser timing algorithm and control method ) 是由 汪松炯 于 2020-04-27 设计创作，主要内容包括：本发明公开了一种飞行时间核酸质谱激光时序算法及控制方法,包括以下步骤,S1,MTC-II主板和CPM-II主板MCU初始化；S2,然后判断真空度是否达标,如果是,则进行如下操作,使能信号触发MCU寄存器置1；然后MCU解锁功能；最后上传信号至上位机,软件解锁对应功能；如果否,则进行如下S3操作。该飞行时间核酸质谱激光时序控制方法保证在真空室真空度足够的情况下进行激光激发,延时加速质谱分析样品,保证实验的准确性和分辨率。(The invention discloses a time-of-flight nucleic acid mass spectrum laser timing algorithm and a control method, which comprises the following steps of S1, initializing an MTC-II mainboard and a CPM-II mainboard MCU; s2, judging whether the vacuum degree reaches the standard, if so, performing the following operation, and triggering an MCU register 1 by an enable signal; then MCU unblocks the function; finally, uploading a signal to an upper computer, and unlocking corresponding functions by software; if not, the following operation S3 is performed. The time-of-flight nucleic acid mass spectrum laser time sequence control method ensures laser excitation under the condition that the vacuum degree of a vacuum chamber is enough, delays and accelerates mass spectrometry of samples, and ensures the accuracy and resolution of experiments.)

1. A time-of-flight nucleic acid mass spectrum laser timing algorithm is characterized in that: comprises the following steps of (a) carrying out,

s1, initializing the MTC-II mainboard and the CPM-II mainboard MCU;

s2, judging whether the vacuum degree reaches the standard or not,

if yes, the following operation is carried out, and the enabling signal triggers the MCU register 1; then MCU unblocks the function; finally, uploading a signal to an upper computer, and unlocking corresponding functions by software;

if not, the following operation S3 is performed;

s3, the MCU controls signal output and pulls down the main power DC-DC enable pin;

s4, the main power supply 25V path does not output voltage, and all high-voltage power supplies are turned off;

s5, locking the laser function of the MCU program;

s6, triggering the MCU by the enable signal and uploading the upper computer software to lock the laser and realize the spotting function;

s7, controlling the diaphragm pump to vacuumize by the MCU;

s8, determining whether the rotating speed reaches 13000, if not, continuously vacuumizing by the vacuum pump until the rotating speed reaches 13000; if yes, the molecular pump continues to pump vacuum, and whether the vacuum value reaches 5x10 is determined^-6(ii) a If not, the molecular pump continues to vacuumize; if so, the following operation S9 is performed;

s9, the MCU unlocks the laser and the spotting function;

s10, uploading a signal to an upper computer, and unlocking the laser and spotting functions by software;

and S11, entering a normal experiment mode.

2. The time-of-flight nucleic acid mass spectrometry laser timing algorithm of claim 1, wherein: the normal test mode in step S11, including the steps of,

step 1, an upper computer outputs functional signals of needle washing, sample transferring and sample application;

step 2, the CPM-II controls a motor of an injection pump and the like to perform functions of needle washing, sample transferring, sample application and the like;

step 3, the upper computer outputs a laser signal and a PIE delay signal;

step 4, the MCU controls the PIE signal output to be connected with the MOSFET in series for conduction, 3KV is pulled down, the ion network is changed into 17KV, and 3KV differential pressure is formed between the ion network and 20KV of the chip frame to provide energy for ion flight;

step 5, the ions fly to an ion detector for ion detection;

step 6, the ion signals are transmitted to an analog-to-digital conversion card for analog-to-digital conversion and coding;

step 7, the upper computer performs algorithm processing and mass spectrometry;

and 8, finishing the mass spectrogram and printing the mass spectrogram.

3. A time-of-flight nucleic acid mass spectrum laser time sequence control method is characterized in that: the method comprises the following steps that voltage is output when the whole machine is powered on, when the MCU detects that the vacuum degree is insufficient through a vacuum gauge, the MCU outputs a high-level signal through I/0 control, a triode is conducted, the voltage is instantly reduced through an accelerating circuit, so that the enabling end EN of the DC-DC closes the function of a DC-DC chip, a light-emitting diode at the later stage is firstly lightened and then extinguished, whether a power module normally works is judged, whether the vacuum degree reaches the standard is judged, if not, the vacuum is continuously pumped until the vacuum degree reaches the standard, and the MCU, an upper computer related laser and a spotting function are locked after the vacuum degree reaches the standard; then the master control MCU outputs a vacuumizing signal high-level signal, the diaphragm pump starts to work and vacuumize, the vacuum gauge monitors and feeds back a vacuum value in real time, and the molecular pump starts to work after the rotating speed reaches the standard until the vacuum value reaches 5x10^-6The vacuum gauge outputs a high level signal, the MCU register signal is set to be 1, and the upper computer is uploaded, and the upper computer and the MCU are unlocked; finally, when a standard experiment is carried out, point application and sample shifting are completed through the upper computer, the upper computer outputs laser signals at the moment, the laser excites crystal bodies of the samples, the PIE time delay signal outputs to lower the voltage of 3KV, so that the generated 3KV voltage difference ions are accelerated, then the ions vertically fly in the vacuum flying tube under the guarantee of lens5KV, and the completion of the vertical flight of the ions in the vacuum flying tube is completedAfter flying, ions reach the ion detector and the analog-digital conversion card for analog-digital conversion, and the upper computer performs mass spectrometry to obtain a mass spectrogram and complete the whole experimental process.

The technical field is as follows:

the invention relates to the field of molecular diagnosis nucleic acid mass spectrometry, in particular to a time-of-flight nucleic acid mass spectrometry laser timing algorithm and a control method.

Background art:

the technical scheme of the existing flight time nucleic acid mass spectrometer adopts a laser synchronous signal to control an analog-digital conversion card and a high-voltage module to achieve a time delay effect, the high-voltage module is opened all the time, and simultaneously, an upper computer and an MCU have no functions of locking a laser, sample application and the like, so that the experiment under low vacuum degree is easily caused, the experiment data is inaccurate, the laser synchronous signal is poor, the capture and the widening are required, the control is troublesome in the whole time delay process, and the accuracy is low; no lens voltage is arranged at the two ends of the flight tube, ions are easy to deviate in flight, and the ions finally stay on the inner wall of the flight tube.

The invention content is as follows:

the invention aims to solve the technical problem of providing a time-of-flight nucleic acid mass spectrometry laser timing algorithm which can ensure that laser excitation is carried out under the condition that the vacuum degree of a vacuum chamber is enough, the sample analysis of mass spectrometry is accelerated in a delayed manner, and the accuracy and the resolution of an experiment are ensured.

The technical scheme of the invention is to provide a time-of-flight nucleic acid mass spectrum laser timing algorithm, which comprises the following steps,

s1, initializing the MTC-II mainboard and the CPM-II mainboard MCU;

s2, judging whether the vacuum degree reaches the standard or not,

if yes, the following operation is carried out, and the enabling signal triggers the MCU register 1; then MCU unblocks the function; finally, uploading a signal to an upper computer, and unlocking corresponding functions by software;

if not, the following operation S3 is performed;

s3, the MCU controls signal output and pulls down the main power DC-DC enable pin;

s4, the main power supply 25V path does not output voltage, and all high-voltage power supplies are turned off;

s5, locking the laser function of the MCU program;

s6, triggering the MCU by the enable signal and uploading the upper computer software to lock the laser and realize the spotting function;

s7, controlling the diaphragm pump to vacuumize by the MCU;

s8, determining whether the rotating speed reaches 13000, if not, continuously vacuumizing by the vacuum pump until the rotating speed reaches 13000; if yes, the molecular pump continues to pump vacuum, and whether the vacuum value reaches 5x10 is determined^-6(ii) a If not, the molecular pump continues to vacuumize; if so, the following operation S9 is performed;

s9, the MCU unlocks the laser and the spotting function;

s10, uploading signal to an upper computer, and unlocking laser and spotting functions by software

And S11, entering a normal experiment mode, fully opening the high-voltage module, and unlocking the laser and sample application functions.

The normal test mode in step S11, preferably, includes the steps of,

step 1, an upper computer outputs functional signals of needle washing, sample transferring and sample application;

step 2, CPM-II controls a motor and the like to perform functions of needle washing, sample transferring, sample application and the like;

step 3, the upper computer outputs a laser signal and a PIE delay signal;

step 4, the MCU controls the PIE signal output to be connected with the MOSFET in series for conduction, 3KV is pulled down, the ion network is changed into 17KV, and 3KV differential pressure is formed between the ion network and 20KV of the chip frame to provide energy for ion flight;

step 5, the ions fly to an ion detector for ion detection;

step 6, the ion signals are transmitted to an analog-to-digital conversion card for analog-to-digital conversion and coding;

step 7, the upper computer performs algorithm processing and mass spectrometry;

and 8, finishing the mass spectrogram and printing the mass spectrogram.

The invention also provides a time-of-flight nucleic acid mass spectrum laser time sequence control method, which comprises the following operations that voltage is electrically output on the whole machine, when the MCU detects that the vacuum degree is insufficient through a vacuum gauge, the MCU outputs a high-level signal through I/0 control, the triode is conducted, the voltage is instantly pulled down through an accelerating circuit, so that the enabling end EN of the DC-DC closes the function of the DC-DC chip, the light-emitting diode at the later stage is firstly turned on and then turned off, whether the power module normally works is judged, whether the vacuum degree reaches the standard is judged, if not, the vacuum is continuously pumped until the vacuum degree reaches the standard, and the MCU, the upper computer related laser and the sample application function are locked after the vacuum degree reaches the standard; then the master control MCU outputs a vacuumizing signal high-level signal, the diaphragm pump starts to work and vacuumize, the vacuum gauge monitors and feeds back a vacuum value in real time, and the molecular pump starts to work after the rotating speed reaches the standard until the vacuum value reaches 5x10^-6The vacuum gauge outputs a high level signal, the MCU register signal is set to be 1, and the upper computer is uploaded, and the upper computer and the MCU are unlocked; finally, when a standard experiment is carried out, point application and sample shifting are completed through the upper computer, the upper computer outputs laser signals at the moment, the laser excites crystal bodies of the samples, the PIE time delay signal outputs to lower the voltage of 3KV, so that the generated 3KV voltage difference ions are accelerated, and then the ions are vacuumized under the guarantee of lens5KVThe flight tube can vertically fly, ions can arrive at the ion detector after the flight is finished, the analog-to-digital conversion card carries out analog-to-digital conversion, and the upper computer carries out mass spectrometry to obtain a mass spectrogram and finish the whole experimental process.

Compared with the prior art, the invention has the following advantages after adopting the scheme: in the time sequence control, a vacuum degree judgment is set, when the vacuum degree is insufficient, the main power supply 25V output is turned off, the high-voltage power supply has no voltage output, the functions of a laser, sample application, sample moving and the like are locked, and the personnel safety, the power consumption and the experimental accuracy of the whole machine are ensured; the 3KV of an off-network is pulled down by adopting a time-delay chip optical coupling isolation control MOSFET, the potential of the point is 17KV relative to the 20KV of the chip frame, and 3KV voltage drop is generated for time-delay acceleration; the method has the advantages that the LENS voltage is increased when ions fly in the vacuum flight tube, so that the ions can fly vertically upwards, laser excitation is finally realized under the condition that the vacuum degree of the vacuum chamber is enough, the mass spectrometry sample is accelerated in a delayed manner, and the accuracy and the resolution of an experiment are ensured.

Description of the drawings:

FIG. 1 is a flow chart of the laser firing timing algorithm of the present invention.

FIG. 2 is a diagram of laser firing timing control according to the present invention.

The specific implementation mode is as follows:

the invention will be further described with respect to specific embodiments in conjunction with the following drawings: