阅读说明：本技术 一种含溴虫氟苯双酰胺和氟啶虫酰胺的杀虫组合物及其应用 (Insecticidal composition containing brofenbendiamide and flonicamid and application thereof ) 是由 熊利鹏 李蕾 刘润峰 余思策 赵悬豪 张瑜 叶小妹 于 2021-07-24 设计创作，主要内容包括：本公开涉及一种含溴虫氟苯双酰胺和氟啶虫酰胺的杀虫组合物及其应用,属于农药杀虫组合物技术领域。上述杀虫组合物包括溴虫氟苯双酰胺和氟啶虫酰胺,所述溴虫氟苯双酰胺和氟啶虫酰胺的重量比为1:1-50。本发明的杀虫组合物,可有效防治各种咀嚼式、刺吸式口器害虫,如鳞翅目、鞘翅目、白蚁、蚜虫科、蓟马科以及蚊蝇等害虫,适用于多叶及结果植物、土豆、大豆、棉花、玉米和豆类,以及水稻、小麦、玉米等多种大田作物。本发明组合物,与现有的单一制剂相比,杀虫效果明显提高,且持效期长,对作物安全,值得在农业生产上推广应用。(The invention relates to an insecticidal composition containing bromofenoxaprop-p-fluorobenzene bisamide and flonicamid and application thereof, and belongs to the technical field of pesticide insecticidal compositions. The insecticidal composition comprises the brotrochan diflufenican and flonicamid in a weight ratio of 1: 1-50. The insecticidal composition can effectively control various chewing and piercing-sucking mouthpart pests, such as lepidoptera, coleoptera, termite, aphididae, thrips, mosquitoes and flies and the like, and is suitable for leafy and fruiting plants, potatoes, soybeans, cotton, corns and beans, and various field crops such as rice, wheat, corns and the like. Compared with the existing single preparation, the composition provided by the invention has the advantages that the insecticidal effect is obviously improved, the persistent period is long, the composition is safe to crops, and the composition is worthy of popularization and application in agricultural production.)

1. The insecticidal composition is characterized by comprising an active pesticide ingredient, wherein the active pesticide ingredient is brobendiamide and flonicamid, and the weight ratio of the brobendiamide to the flonicamid is 1: 1-50.

2. The insecticidal composition containing the flonicamid and the flonicamid as claimed in claim 1, wherein the weight ratio of the flonicamid to the flonicamid is 1: 1-30.

3. The insecticidal composition containing the flonicamid and the flonicamid as claimed in claim 2, wherein the weight ratio of the flonicamid to the flonicamid is 1: 1-10.

4. The insecticidal composition containing the brevibrambutan fluorobenzodiamide and the flonicamid as claimed in any one of claims 1 to 3, wherein the insecticidal composition further comprises an auxiliary ingredient, and the content of the auxiliary ingredient is 1 to 90 parts by weight relative to 1 part by weight of the active ingredient.

5. The insecticidal composition containing flubendiamide and flonicamid as claimed in claim 4, wherein the content of the auxiliary ingredient is 15-80 parts by weight with respect to 1 part by weight of the active ingredient.

6. The insecticidal composition containing the brevibrambutan fluorobenzene bisamide and the flonicamid as claimed in claim 5, wherein the auxiliary ingredients comprise at least one of an emulsifier, a wetting agent, a dispersing agent, a thickening agent, an antifreezing agent, a preservative, a wall material, a film forming agent, a color paste, an antifoaming agent, water, an organic solvent, a disintegrating agent, a binder and a solid inert carrier.

7. The insecticidal composition containing the brevibrambutan fluorobenzene bisamide and the flonicamid as claimed in claim 6, wherein the dosage form of the insecticidal composition is suspending agent, missible oil, oil suspending agent, microemulsion, aqueous emulsion, microcapsule suspending agent, aqueous solution, wettable powder, water dispersible granule or seed treatment agent.

8. The use of the insecticidal composition containing the flonicamid and the flonicamid as claimed in any one of claims 1 to 7, which is used for controlling pests on crops such as fruits, vegetables and fields. In particular to control chewing mouthpart pests such as diamond back moth, beet armyworm, chilo suppressalis, rice leaf roller and the like, and piercing-sucking mouthpart pests such as liriomyza sativae, thrips, bemisia tabaci, aphids and the like.

9. The use of claim 8, wherein the field crop is selected from the group consisting of potato, soybean, cotton, corn, legumes, rice, wheat, corn, and the like.

Technical Field

The disclosure relates to the technical field of pesticides, and particularly relates to an insecticidal composition containing bromofenoxanil and flonicamid and application thereof in pest control.

Background

Bromofenoxanil, english name: the molecular structure of Broflanilide is as follows:

is a bisamide insecticide developed by Mitsui agricultural chemical company, and the brofenpyrad fluorobenzene bisamide is an insecticide with a novel action mechanism: GABA-gated chloride channel allosteric modulators. It can cause hyperexcitability and convulsions in insects. Brofenpyrad fluorobenzenediamide was classified by the Insecticide Resistance Action Committee (IRAC) into group 30, which was the only compound in this group to date. Bromoidin fluorobenzenediamide is useful in Insecticide Resistance Management (IRM) applications to control pests resistant to existing insecticides, and exhibits excellent effectiveness in controlling a wide variety of problematic chewing pests, including caterpillars and crustaceans that infest specialty and row crops, as well as non-crop pests such as termites, ants, cockroaches, and flies. It also has potential use in cereals as a seed treatment for nematode control and also on foliar surfaces for leafy and fruiting plants, potatoes, soybeans, cotton, corn and legumes.

Flonicamid, english name: flonicamid, molecular structure:

the insecticidal composition is a novel low-toxicity pyridine amide type insect growth regulating insecticide developed by Nippon Shigaku K.K., wherein flonicamid molecules contain trifluoromethyl and introduce a nicotine amine structure, and the flonicamid has strong neurotoxicity and quick antifeedant effect, has a unique action mechanism and is different from high-efficiency insecticides such as neonicotinoids, pymetrozine and the like. The biological activity of the pesticide is extremely high, and the pesticide can be used for preventing and controlling aphids and other sucking mouthparts pests on wide crops and can also be used for preventing and controlling pests which have resistance to conventional pesticides. Has good safety to crops, people, livestock and environment and has good development prospect.

The long-term use of the pesticide containing the single active ingredient for pest control can bring about different degrees of harm, such as generation of drug resistance of pests, environmental pollution and the like. The insecticide composition can be used for reasonably compounding different active ingredients, and has the effects of expanding an insecticidal spectrum, delaying the duration time and reducing the dosage, so that the medication cost is reduced and the environmental pollution is reduced.

Disclosure of Invention

In order to overcome the problems that a single pesticide in the prior art is easy to generate resistance after being used for a long time, the effect of preventing and controlling pests is reduced, the use amount is increased, the environmental pollution is caused and the like, the disclosure provides a pesticidal composition containing brofenbendiamide and flonicamid and application thereof.

In one aspect, the invention provides an insecticidal composition containing brotrochar bendiamide and flonicamid, which contains active ingredients, wherein the active ingredients are brotrochar bendiamide and flonicamid. Preferably, the weight ratio of the flubendiamide to the flonicamid in the insecticidal composition is 1: 1-50.

Further, the weight ratio of the flubendiamide to the flonicamid in the insecticidal composition is 1: 1-30.

Preferably, the weight ratio of the flubendiamide to the flonicamid in the insecticidal composition is 1:1 to 10, particularly preferably in a ratio of 1: 40,1: 30,1: 20,1: 10,1: 7,1: 4,1: 2,1: 1.

on the other hand, the insecticidal composition of the invention also comprises auxiliary components, and the content of the auxiliary components is 1-90 parts by weight relative to 1 part by weight of the active components; preferably, the content of the auxiliary ingredient is 15 to 80 parts by weight with respect to 1 part by weight of the active ingredient.

Further, the auxiliary ingredients in the insecticidal composition comprise at least one of an emulsifier, a wetting agent, a dispersing agent, a thickening agent, an antifreezing agent, a preservative, a wall material, a film forming agent, a color paste, a defoaming agent, water, an organic solvent, a disintegrating agent, a binder and a solid inert carrier.

Preferably, the formulation of the insecticidal composition provided by the invention is suspending agent, missible oil, oil suspending agent, microemulsion, emulsion in water, microcapsule suspending agent, aqueous solution, wettable powder, water dispersible granule or seed treatment agent.

On the other hand, the invention also provides an application of the insecticidal composition, which is used for preparing a pesticide preparation for preventing and controlling pests, and can be used for preventing and controlling pests on crops such as fruits, vegetables, fields and the like, in particular chewing mouthparts pests such as diamond back moths, beet armyworms, chilo suppressalis, rice leaf rollers and the like, and piercing-sucking mouthparts pests such as liriomyza sativae, thrips, bemisia tabaci and aphids. The field crops refer to potatoes, soybeans, cotton, corns and beans, and various field crops such as rice, wheat, corns and the like.

Compared with the prior art, the invention has the beneficial effects that:

the insecticidal composition containing the brotroche difluoride and the flonicamid has the advantages that the effective components of the brotroche difluoride and the flonicamid are combined, and the two pesticides have obvious synergistic effect.

Compared with the existing single-component pesticide, the insecticidal composition containing the brotroche difluoride and the flonicamid has an obvious insecticidal effect and a synergistic effect, and meanwhile, the insecticidal composition containing the brotroche difluoride and the flonicamid is safe to crops and worthy of popularization and application in agricultural production.

Detailed Description

The present invention will be further described with reference to the following examples.

The instruments, reagents, materials and the like used in the following examples are conventional instruments, reagents, materials and the like in the prior art and are commercially available in a normal manner unless otherwise specified. Unless otherwise specified, the experimental methods, detection methods, and the like described in the following examples are conventional experimental methods, detection methods, and the like in the prior art.

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described with the following embodiments, but the present invention is by no means limited to these examples. The following description is only a preferred embodiment of the present invention, and is only for the purpose of explaining the present invention, and should not be construed as limiting the scope of the present invention. It should be understood that any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

The insecticidal composition can be prepared into any one of dosage forms suitable for agriculture by using a known method, and the better dosage forms are suspending agents, missible oil, oil suspending agents, micro-emulsions, aqueous emulsions, microcapsule suspending agents, aqueous solutions, wettable powder, water dispersible granules or seed treatment agents; all the percentages in the formula are weight percentages, the processing technology of various formulations of the composition is the prior art, and the composition can be changed according to different conditions. The present invention will be further described with reference to the following examples.

Example 1: 40% Chlorantraniliprole, flonicamid suspension (1:7)

The 40% brofenbendiamide-flonicamid suspending agent comprises the following components in parts by weight:

5 wt% of brobendiamide, 35 wt% of flonicamid, 4 wt% of polycarboxylate, 3 wt% of polyoxyethylene polyoxypropylene copolymer, 0.2 wt% of xanthan gum, 0.2 wt% of sodium benzoate, 0.3 wt% of magnesium aluminum silicate, 0.3 wt% of silicone defoamer, 5 wt% of propylene glycol, and water added to 100 wt%.

Example 2: 26% Chlorantraniliprole, flonicamid suspension (1:25)

26% of a bromofenoxaprop-fluorobenzene bisamide-flonicamid suspending agent, wherein the suspending agent comprises the following components in parts by weight:

1 wt% of bromofenoxanil, 25 wt% of flonicamid, 2.5 wt% of alkylphenol polyoxyethylene, 2 wt% of sodium dodecyl sulfate, 0.3 wt% of xanthan gum, 0.3 wt% of sodium benzoate, 0.5 wt% of magnesium aluminum silicate, 0.3 wt% of silicone defoamer, 5 wt% of ethylene glycol, and water added to 100 wt%.

Example 3: 51% brobendiamide-flonicamid water dispersible granule (1:50)

51% brofenbendiamide-flonicamid water dispersible granules, which comprise the following components in parts by weight:

1 wt% of bromofenoxanil, 50 wt% of flonicamid, 6 wt% of sodium dodecyl benzene sulfonate, 8 wt% of sodium lignosulfonate, 15 wt% of dextrin and kaolin added to 100 wt%.

The insecticidal composition comprises the effective components of the brotrochar diflufenican and the flonicamid, and the combination of the two has obvious synergistic effect on insect pest control, not only simple superposition of the effects of the two agents, which can be clearly understood from the following test results.

The inventor carries out synergistic research on mutual compounding of the insecticidal composition of the brobendiamide and the flonicamid. The test adopts rice leaf roller as a test object, and if no special description is given, the following mentioned proportions (including percentage) are weight ratio, and the specific method is as follows:

indoor bioassay screening

1.1 test pests:

the rice leaf roller imagoes bred on rice leaves in a greenhouse are inoculated on rice sprouts growing without insects, spawning and hatching are carried out in an insect breeding room with the indoor temperature of 28 +/-2 ℃, the relative humidity of 55-65% and illumination for 12 hours, and the larvae are bred by the rice leaves until the larvae grow to 3 years old for later use.

1.2 test agent:

98% of bromofenoxaprop-p-fluorobenzamide technical material (AGRO, Mitsui chemical Co., Ltd.) and 96% of flonicamid (Takara Shuzo Co., Ltd.).

1.3 test methods:

referring to the dropping method of the bioassay standard NY/T1154.1-2006, a micro dropper is cleaned by a solvent, and the dropper is adjusted to a standby state. Selecting indoor-fed 3-instar larvae as test insects, and placing the test insects in a 9cm culture dish for later use under mild anesthesia. Dripping the liquid medicine on the forebreast and back plate of the tested insect head by using a micro dropper, wherein 0.5 mu L of liquid medicine is dripped into each head. Transferring the spotted test insects to normal conditions for feeding. Each treatment was repeated 4 times, with no less than 10 test insects per repetition.

1.4 data statistics and analysis:

and (5) checking the death condition of the test insects 24h after treatment, calculating the death rate (taking the non-autonomous reaction of the test insects after light needling as a standard for judging the death of the test insects), and recording the total number of the test insects and the number of dead insects.

From the survey data, corrected mortality for each treatment was calculated. And the co-toxicity coefficient (CTC value) of the mixture was calculated by Sun cloud Peter method with reference to NY/T1154.7-2006.

The corrected mortality is calculated according to the formulas (1) and (2) in percentage (%), and the calculation result retains two digits after decimal point:

in the formula:

P₁-mortality;

k- - -number of dead insects;

n- - -total number of worms treated.

In the formula:

P₂corrected mortality;

P_t-treatment mortality;

P₀mortality of blank control.

If the control mortality rate is less than 5%, not correcting; correcting the control mortality rate between 5% and 20% according to a formula (2); control mortality was > 20%, and the test was redone.

By taking the logarithmic value of the medicament concentration (mg/L) as an independent variable X and the probability value of correcting the death rate as a dependent variable Y, respectively establishing toxicity regression equations, calculating LC50 of single-dose and mixed-dose and co-toxicity coefficients of the mixed-dose by adopting DPS software, and comparing the synergistic conditions. LC50 was calculated to calculate the cotoxicity coefficient (CTC) according to the grandsinope method. The co-toxicity coefficient CTC is calculated according to the following formula:

(A is brotrochar difluoride as a standard medicament, the virulence index is 100, B is flonicamid): the statistical analysis was performed using the DPS statistical analysis software, LC50 was calculated for each drug, and the cotoxicity coefficient (CTC value) of the blend was calculated according to the Sun cloud Peel method.

The virulence index (ATI) was measured as (standard agent LC 50/test agent LC50) × 100.

Theoretical virulence index (TTI) ═ a agent virulence index x percent of a in the mixture + B agent virulence index x percent of B in the mixture.

Co-toxicity coefficient (CTC) × 100 [ measured toxicity index (ATI) of the mixture)/Theoretical Toxicity Index (TTI) of the mixture ].

According to NY/T11547.7-2006 pesticide combination division standard: the co-toxicity coefficient (CTC) is more than or equal to 120, which is the synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80, and the antagonism is shown; 80 < co-toxicity coefficient (CTC) < 120 showed additive effects. The results are shown in Table 1.

TABLE 1 measurement of indoor combined toxicity of Bifenflurdiamide and flonicamid for cnaphalocrocis medinalis

As can be seen from table 1, when the brotrocha bendiamide and the flonicamid are compounded in the mixing ratio of 1:1-50 (mass ratio), the cotoxicity coefficient (CTC) is greater than 120 (especially 1:7), and a synergistic effect is shown on cnaphalocrocis medinalis, so that the reasonability and feasibility of compounding the brotrocha bendiamide and the flonicamid are seen.

In conclusion, the invention adopts the compound composition of two active ingredients, namely the brotrochar diflufenican and the flonicamid, and has a synergistic effect. Compared with single preparation, the insecticidal composition has the advantages of unique action mechanism, small dosage, good quick action, long lasting period and environmental protection. Therefore, the research and the development and the popularization of the invention have great social significance and can generate great economic benefits.