Improved homogeneity of fungal biopolymers grown in void spaces

文档序号:1255339 发布日期:2020-08-21 浏览:8次 中文

阅读说明:本技术 生长在空隙空间中的真菌生物聚合物的提高的均质性 (Improved homogeneity of fungal biopolymers grown in void spaces ) 是由 J·H·卡普兰-贝 I·T·博恩斯蒂尔 L·格里瑟姆 G·R·麦金太尔 于 2018-11-14 设计创作,主要内容包括:使生物聚合物材料生长的方法采用在置于封闭孵育室的容器中孵育包含营养基质和真菌的生长培养基,具有经过每个容器的气流,同时将孵育室保持在湿度、温度、二氧化碳和氧气的预定环境下。可以平行于或垂直于生长培养基的表面引导气流。(The method of growing the biopolymer material employs incubating a growth medium comprising a nutrient substrate and a fungus in containers disposed in an enclosed incubation chamber with a gas flow through each container while maintaining the incubation chamber in a predetermined environment of humidity, temperature, carbon dioxide and oxygen. The gas flow may be directed parallel or perpendicular to the surface of the growth medium.)

1. A method of growing a biopolymer material, comprising the steps of:

providing a plurality of containers, each of said containers defining a cavity containing a growth medium comprising a nutrient substrate and a fungus;

placing the plurality of containers in a closed incubation chamber;

maintaining the closed incubation chamber in a predetermined environment of humidity, temperature, carbon dioxide and oxygen, the predetermined environment being sufficient to produce mycelium biopolymers while preventing the fungi from fully differentiating into mushrooms;

directing a gas stream containing a high carbon dioxide content through the incubation chamber to pass through the growth medium in each of the containers; and

incubating the growth medium in each of the containers for a period of time sufficient for the fungus to digest the nutrient substrate and produce a mycelium biopolymer consisting entirely of fungal mycelium in each of the containers.

2. The method of claim 1, wherein the airflow is directed into the enclosed incubation chamber transverse to the container.

3. The method of claim 1, wherein the airflow is directed into the enclosed incubation chamber perpendicular to the container.

4. The method of claim 1, wherein the plurality of containers are stacked in a plurality of vertically spaced rows within the incubation chamber.

5. The method of claim 4, wherein the environment is maintained at 99% Relative Humidity (RH), 5% CO during the incubating step2And 85 DEGA fluctuating temperature of F-90 deg.F.

6. The method of claim 5, wherein the airflow is directed into the enclosed incubation chamber transverse to the container.

7. The method of claim 5, wherein the airflow is directed into the enclosed incubation chamber perpendicular to the container.

8. The method of claim 1, wherein the airflow is pulsed during the incubating step.

9. The method of claim 1, wherein the gas stream contains a carbon dioxide content of at least 5% to 7% by volume.

10. A method of growing a biopolymer material, comprising the steps of:

providing a plurality of containers, each of said containers defining a cavity containing a growth medium comprising a nutrient substrate and a fungus;

placing the plurality of containers in a closed incubation chamber;

maintaining the closed incubation chamber in a predetermined environment of humidity, temperature, carbon dioxide and oxygen;

dispensing mist through the incubation chamber to pass through the growth medium in each of the containers; and

incubating the growth medium in each of the containers for a period of time sufficient for the fungus to digest the nutrient substrate and produce a mycelium biopolymer in each of the containers that consists entirely of fungal mycelium without substantial morphological changes.

11. The method of claim 9, wherein the mist comprises moisture and a solute.

12. The method of claim 10, wherein the solute is a mineral.

13. The method of claim 9, wherein during the incubating step, aerial hyphae grow out of each of the containers, and the fog is dispensed on top of the aerial hyphae in a regulated amount and/or distribution of solutes to achieve a predetermined material density and material homogeneity.

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