阅读说明：本技术 一种海参美容保健品及其制备方法 (Sea cucumber beauty health product and preparation method thereof ) 是由 张红 于 2021-06-29 设计创作，主要内容包括：本发明提供了一种海参美容保健品的制备方法,属于保健品技术领域,包括以下步骤：S1、将新鲜的海参洗净,浸泡；S2、研磨处理得到海参浆液,放入无菌反反应釜中,加入苏打水和花青素,冷藏；S3、加入木聚糖酶和蛋白酶,搅拌,升温,酶解反应；S4、将大麦、桃胶、化橘红分别进行研磨成粉末；将糙米用文火烘炒,研磨成糙米浆液；S5、在反应釜中加入大麦、桃胶、化橘红粉末和糙米浆液,再加入研磨成粉末的桃胶和化橘红,匀质,出料、灌装,得到成品。本发明能够在相对温和的条件下使得海参蛋白得到较高程度的水解,并保留尽可能多的活性成分,达到美容保健的效果。(The invention provides a preparation method of a sea cucumber beauty and health care product, belonging to the technical field of health care products and comprising the following steps: s1, cleaning and soaking fresh sea cucumbers; s2, grinding to obtain sea cucumber slurry, putting the sea cucumber slurry into a sterile reverse reaction kettle, adding soda water and anthocyanin, and refrigerating; s3, adding xylanase and protease, stirring, heating, and carrying out enzymolysis reaction; s4, respectively grinding the barley, the peach gum and the pummelo peel into powder; parching brown rice with slow fire, and grinding into brown rice pulp; s5, adding barley, peach gum, pummelo peel powder and brown rice slurry into a reaction kettle, adding the peach gum and pummelo peel which are ground into powder, homogenizing, discharging and filling to obtain the finished product. The invention can hydrolyze the sea cucumber protein to a higher degree under relatively mild conditions, and retain as much as possible of active ingredients, thereby achieving the effects of beauty treatment and health care.)

1. A preparation method of a sea cucumber beauty and health care product is characterized by comprising the following steps: the method comprises the following steps:

s1, taking out viscera of fresh sea cucumber, cleaning, putting into a normal-temperature stirring pot, adding a proper amount of glacial acetic acid, starch and water into the stirring pot, soaking for 25-45min, then stirring at a low speed for 6-8min, taking out the sea cucumber, and washing again for later use;

s2, grinding the sea cucumbers processed in the step S1 at a high speed to obtain sea cucumber slurry, putting the sea cucumber slurry into a sterile reverse reaction kettle, adding soda water and anthocyanin, stirring and uniformly mixing, transferring into a refrigerating chamber at the temperature of-5-4 ℃, standing and refrigerating for 2-3 hours, and transferring out of the refrigerating chamber for later use;

s3, adding xylanase and protease into the sea cucumber slurry mixture treated in the step S2, stirring, slowly heating to 35-45 ℃, preserving heat, continuously and slowly stirring, and carrying out enzymolysis reaction for 4-5 hours under the condition that the pressure is 60-90 KPa;

s4, respectively grinding the barley, the peach gum and the pummelo peel into powder for later use; parching brown rice with slow fire until the surface is yellowish, cooling, adding small amount of purified water, and grinding into brown rice pulp;

s5, adding the barley, peach gum, pummelo peel powder and brown rice pulp for later use one by one in a reaction kettle while stirring, keeping the temperature at 35-45 ℃, continuously and slowly stirring for reaction for 1 hour, adding the peach gum and pummelo peel which are ground into powder and purified water, stopping stirring, cooling to room temperature, homogenizing for 3-5min, discharging, and filling to obtain the finished product.

2. The preparation method of the sea cucumber beauty and health care product according to claim 1, which is characterized by comprising the following steps: the adding ratio of the protease to the xylanase is 5: 1.

3. The preparation method of the sea cucumber beauty and health care product according to claim 1, which is characterized by comprising the following steps: the protease comprises a mixed enzyme of subtilisin, trypsin and papain, and the addition ratio of the subtilisin, the trypsin and the papain is 2:1: 1.

4. The preparation method of the sea cucumber beauty and health care product according to claim 1, which is characterized by comprising the following steps: the step S4 further includes grinding the angelica, lily, medlar, papaya and dried longan into powder, and adding the powder, peach gum and pummelo peel into the reaction kettle in the step S5.

5. The preparation method of the sea cucumber beauty and health care product according to claim 1, which is characterized by comprising the following steps: and in the step S5, after homogenizing, performing activated carbon adsorption and filtration during discharging, and then filling to obtain a finished product.

6. The preparation method of the sea cucumber beauty and health care product according to claim 1, which is characterized by comprising the following steps: in the step S3, slowly heating to 42 ℃, keeping the temperature and continuously and slowly stirring, and carrying out enzymolysis reaction for 4.5 hours under the condition that the pressure is 80 KPa;

and in the step S2, the food is transferred into a 0 ℃ refrigerating chamber for standing and refrigerating for 2.5 hours, and then the food is transferred out of the refrigerating chamber for standby.

7. A sea cucumber beauty health product is characterized in that: the brown rice is baked and fried brown rice.

8. The sea cucumber beauty treatment health product according to claim 7, characterized in that: the composition comprises the following substances in parts by weight: 10.0 to 25.0 parts of sea cucumber, 1.0 to 5.0 parts of barley, 0.5 to 2.0 parts of anthocyanin, 0.5 to 2.0 parts of peach gum, 1.0 to 3.0 parts of pummelo peel, 1.0 to 3.0 parts of brown rice and 8.0 to 14.0 parts of soda water.

9. The sea cucumber beauty treatment health product according to claim 7, characterized in that: also comprises angelica, lily, medlar, pawpaw, dried longan and purified water.

10. The sea cucumber beauty treatment health product according to claim 9, characterized in that: the composition comprises the following substances in parts by weight: 0.5 to 2.0 parts of angelica, 0.5 to 2.0 parts of lily, 0.5 to 2.0 parts of medlar, 1.0 to 3.0 parts of pawpaw and 1.0 to 3.0 parts of dried longan.

Technical Field

The invention relates to the technical field of health products, and particularly belongs to a sea cucumber beauty health product and a preparation method thereof.

Background

Sea cucumber, distinguished in the seafood taste of various delicacies, is rich in more than 50 nutrient components required by human body, such as amino acids, vitamins and chemical elements, and also contains various bioactive substances, such as acidic mucopolysaccharide, saponin, collagen and the like. For example, holothurian polysaccharide has various pharmacological activities including anti-tumor, anticoagulation, immunoregulation and anti-thrombosis, blood fat reduction and the like, and holothurian saponin has various biological activities of improving immunity, resisting bacteria, resisting tumors, resisting cancers and the like, has warm nature and tonification, is capable of abusing ginseng, tonifying kidney channels, eliminating phlegm, benefiting essence, tonifying yang, treating flaccidity and the like. The sea cucumber contains 18 amino acids, wherein the contents of glycine, glutamic acid and arginine are far higher than those of other amino acids, and also contains vitamins such as VB2, VA, VB1, VB6, VE, VD, VK and the like, which cannot be synthesized by the human body, and the types of essential fatty acids in the sea cucumber are complete, such as linoleic acid, linolenic acid, EPA, DHA and the like, so the sea cucumber becomes an rare health-care product.

However, the sea cucumber contains abundant nutritional active substances, but is not easy to digest, the protein component in the sea cucumber belongs to collagen, is similar to the protein structure of animal skin, is a white, unbranched and opaque fibrous protein, has a 3-strand helical structure, forms a left-hand helical structure through 3 polypeptide chains, and is mutually occluded into a firm right-hand supercoiled structure through hydrogen bonds, so that a right-hand composite helical structure formed by mutually winding left-handed a chains is formed, and the structure can form firm combination in vivo, is not easy to separate and is not easy to digest. Therefore, in order to facilitate digestion and absorption of proteins in sea cucumbers, high-temperature and ultrahigh-pressure treatment, ultralow-temperature and ultralow-pressure treatment in modern processes, a long-time decoction mode in a traditional mode and the like are generally needed, so that the proteins are inactivated and denatured fully, and the proteins are digested and absorbed better. However, this leads to a large loss of nutrients in the sea cucumber, such as vitamins, sea cucumber polysaccharides, sea cucumber saponins, some beneficial fatty acids, and other active substances, and the affected substances in the sea cucumber cannot be fully and effectively utilized.

The sea cucumber needs to be specially noted in the processing process, alkaline substances are added for too long time or randomly when the sea cucumber is washed in the preparation process of the sea cucumber health-care product at present, so that the sea cucumber is too long to leave the sea water or is easy to activate autolytic enzyme by alkali, self-melting is carried out to form a pool of liquid, a large amount of nutrient substances are lost, the space structure of collagen is changed in an acidic environment, and protein molecules are aggregated and contracted to different degrees, so that the control of conditions in the preparation process of the sea cucumber health-care product is quite important, and the sea cucumber health-care product prepared in the market at present can not fully utilize the sea cucumber to exert the real effect.

In addition, the sea cucumber is rich in nutrition, but the sea cucumber health care products in the current market are matched with various substances, even with ginseng, cordyceps sinensis and the like, so that the absorption of the sea cucumber nutrient substances is influenced, the liver fire of a human body is vigorous, deficiency is not compensated, and the physical quality is easily reduced due to long-term food. Therefore, the matching of the sea cucumber health care product needs to be proper and simplified, so that the excellent efficacy of the sea cucumber health care product can be exerted to the maximum extent.

The sea cucumber health care product at present can be added with a lot of sugar, and is not suitable for old people, because sea cucumber saponin has the effects of improving immunity, resisting tumors, inhibiting human cancer cells and acting on nerve cells, can prevent and treat epilepsy, but is bitter in taste and easy to influence the edible taste and flavor, so that various sugar or sweeteners can be added in the preparation process of a lot of merchants for regulation, but is very bad for health.

Disclosure of Invention

Aiming at the defects and shortcomings in the background technology, the invention provides a preparation method of a sea cucumber beauty and health care product, which can hydrolyze sea cucumber protein to a higher degree under relatively mild conditions, and retain as many active ingredients as possible, thereby achieving the effects of beauty and health care.

The invention also aims to provide a preparation method of the sea cucumber beauty and health care product, which has mild and safe components, can promote the sea cucumber protein to be hydrolyzed to a higher degree, is mild to intestines and stomach, and can achieve the effects of beauty and health care.

In order to realize the purpose, the invention adopts the following technical scheme to realize the purpose:

a preparation method of a sea cucumber beauty and health care product comprises the following steps:

s1, taking out viscera of fresh sea cucumber, cleaning, putting into a normal-temperature stirring pot, adding a proper amount of glacial acetic acid, starch and water into the stirring pot, soaking for 25-45min, then stirring at a low speed for 6-8min, taking out the sea cucumber, and washing again for later use; the method has the advantages that glacial acetic acid and starch are added during sea cucumber cleaning, so that the sea cucumber can be cleaned quickly, an acidic environment is kept, quick cleaning is promoted, autolysis of the sea cucumber can be effectively avoided, and nutritional active substances in the sea cucumber are protected.

S2, grinding the sea cucumbers processed in the step S1 at a high speed to obtain sea cucumber slurry, putting the sea cucumber slurry into a sterile reverse reaction kettle, adding soda water and anthocyanin, stirring and uniformly mixing, transferring into a refrigerating chamber at the temperature of-5-4 ℃, standing and refrigerating for 2-3 hours, and transferring out of the refrigerating chamber for later use; the full mixing of the soda water and the anthocyanin with the sea cucumber slurry is promoted while the sea cucumber is refrigerated and kept fresh, the control of the refrigerating time is important, and the full mixing can be promoted within 2-3 hours, so that the protein is softened, the enzymolysis is easier, and the nutritional active ingredients can be protected.

S3, adding xylanase and protease into the sea cucumber slurry mixture treated in the step S2, stirring, slowly heating to 35-45 ℃, preserving heat, continuously and slowly stirring, and carrying out enzymolysis reaction for 4-5 hours under the condition that the pressure is 60-90 KPa; the loss of active ingredients can be avoided under relatively mild conditions, the protein in the sea cucumber can be hydrolyzed, nutrient substances are released, the method is far more efficient than the conventional method which utilizes the ultrahigh pressure of more than 0.1MPa and enzymolysis for more than 8 hours, and the nutritional active ingredients can be better protected.

S4, respectively grinding the barley, the peach gum and the pummelo peel into powder for later use; parching brown rice with slow fire until the surface is yellowish, cooling, adding small amount of purified water, and grinding into brown rice pulp; grinding into powder or slurry can promote thorough mixing, and the brown rice is processed by baking and parching, and has effects of removing fishy smell and cold of Stichopus japonicus, protecting liver and invigorating stomach.

S5, adding the barley, peach gum, pummelo peel powder and brown rice pulp for later use one by one in a reaction kettle while stirring, keeping the temperature at 35-45 ℃, continuously and slowly stirring for reaction for 1 hour, adding the peach gum and pummelo peel which are ground into powder and purified water, stopping stirring, cooling to room temperature, homogenizing for 3-5min, discharging, and filling to obtain the finished product.

Further measures taken are: the adding ratio of the protease to the xylanase is 5: 1.

Further measures taken are: the protease comprises a mixed enzyme of subtilisin, trypsin and papain, and the addition ratio of the subtilisin, the trypsin and the papain is 2:1: 1.

Further measures taken are: the step S4 further includes grinding the angelica, lily, medlar, papaya and dried longan into powder, and adding the powder, peach gum and pummelo peel into the reaction kettle in the step S5.

Further measures taken are: and in the step S5, after homogenizing, performing activated carbon adsorption and filtration during discharging, and then filling to obtain a finished product.

Further measures taken are: in the step S3, slowly heating to 42 ℃, keeping the temperature and continuously and slowly stirring, and carrying out enzymolysis reaction for 4.5 hours under the condition that the pressure is 80 KPa;

and in the step S2, the food is transferred into a 0 ℃ refrigerating chamber for standing and refrigerating for 2.5 hours, and then the food is transferred out of the refrigerating chamber for standby.

A sea cucumber beauty and health care product comprises sea cucumber, barley, anthocyanin, peach gum, pummelo peel, brown rice and soda water, wherein the brown rice is baked and fried brown rice.

Further measures taken are: the composition comprises the following substances in parts by weight: 10.0 to 25.0 parts of sea cucumber, 1.0 to 5.0 parts of barley, 0.5 to 2.0 parts of anthocyanin, 0.5 to 2.0 parts of peach gum, 1.0 to 3.0 parts of pummelo peel, 1.0 to 3.0 parts of brown rice and 8.0 to 14.0 parts of soda water.

Further measures taken are: also comprises angelica, lily, medlar, pawpaw, dried longan and purified water.

Further measures taken are: the composition comprises the following substances in parts by weight: 0.5 to 2.0 parts of angelica, 0.5 to 2.0 parts of lily, 0.5 to 2.0 parts of medlar, 1.0 to 3.0 parts of pawpaw and 1.0 to 3.0 parts of dried longan.

In order to fully utilize the nutrient substances in the sea cucumber and reduce the loss of active substances, the invention develops a preparation method of the sea cucumber health care product through long-time research and continuous trial and exploration. In addition, in the whole preparation process, the control of each link is proper, so that the autolysis reaction can be avoided in the early stage, and the separation and enzymolysis can be efficiently carried out in the later stage, thereby being beneficial to digestion and absorption.

In order to convert substances which are difficult to digest and absorb in the sea cucumber into small molecular substances which are easy to absorb under a relatively mild environment and retain active substances in the sea cucumber, the invention carries out a great innovation on the use of enzyme and utilizes xylanase which is ignored in the field. The xylanase is widely applied to the beer brewing process, however, people are difficult to be connected with the enzymolysis process of collagen, even general mixing can influence the enzymolysis effect of protease on the collagen, and therefore, the xylanase is more difficult to be applied to the large-scale treatment process of sea cucumbers. However, the invention discovers that the enzymolysis is carried out on the sea cucumber slurry treated by soda water and anthocyanin in a certain proportion by xylanase and protease, so that the excellent enzymolysis effect can be exerted under the environment of high temperature and ultrahigh pressure, the collagen in the sea cucumber can be quickly subjected to enzymolysis, the xylanase can be combined with the protease to a certain extent, the release of other active substances in the sea cucumber can be accelerated without damaging the active substances, and after repeated experiments, the nutrient substances in the sea cucumber can be separated only by carrying out enzymolysis reaction for 4-5 hours under the condition that the pressure is 60-90 KPa, which is far more than 0.1MPa of the traditional mode by utilizing ultrahigh pressure, and the enzymolysis is more efficient than 8 hours.

In addition, glacial acetic acid and starch are added during sea cucumber cleaning, so that the sea cucumber can be cleaned quickly, an acidic environment is kept, quick cleaning is promoted, autolysis of the sea cucumber can be effectively avoided, and nutritional active substances in the sea cucumber are protected; and when the material is discharged, the material is firstly adsorbed and filtered by the active carbon, so that particles and impurities can be effectively removed, and the purity and quality of the product are ensured.

The invention adds the soda water and the anthocyanin, and also adds the barley, the peach gum, the pummelo peel and the brown rice. The brown rice is parched, so that fishy smell and cold of sea cucumber can be removed, and liver and stomach can be protected. The sea cucumber health product is milder, can invigorate the stomach and promote the absorption of nutrient substances of the sea cucumber by adding the barley, and meanwhile, xylan and pentosan in the barley and brown rice can be decomposed and converted by xylanase to obtain xylooligosaccharide, and the xylooligosaccharide has fresh and sweet taste, pure sweetness and low viscosity, is not sweet and greasy like cane sugar, can effectively adjust the integral taste and flavor, and is difficult to decompose xylooligosaccharide in various digestive juice in the digestive tract of a human body, so that the decomposed xylooligosaccharide does not influence the blood sugar concentration, does not increase the level of insulin in blood sugar, does not form fat deposition and the like, is healthier, can avoid the influence on the health of the human body caused by adding cane sugar, white sugar and the like in the traditional mode, and also has sweet and greasy taste.

The skin care product also has the interaction of the active substances in the peach gum, the pummelo peel and the sea cucumber, and can also be used for maintaining beauty and keeping young, exerting excellent effects of resisting wrinkles and tendering skin and revitalizing the skin. The dried pawpaw and the dried longan have the flavor adjusting function, the whole formula is simple and mild, the matching is reasonable, the digestion and absorption of nutrient substances can be promoted, and the situation that the nutrition is excessive or the stimulation is caused to the human body due to the complex addition and matching can be avoided.

Through the technical scheme, compared with the prior art, the invention has the following beneficial effects:

1. the invention can fully separate the protein in the sea cucumber in a relatively mild environment, can furthest reserve the active substances in the sea cucumber, is proper in the control of each link in the whole preparation process, can avoid autolysis reaction in the early stage, and can efficiently separate and carry out enzymolysis in the later stage so as to be beneficial to digestion and absorption.

2. According to the invention, the taste and flavor can be adjusted without adding sweeteners such as sucrose, white sugar and the like, and by skillfully utilizing the interaction of the xylanase in the treatment process of the barley, the brown rice and the sea cucumber, the nutrition substances separated from the sea cucumber can be promoted to be absorbed by intestines and stomach, and meanwhile, the xylooligosaccharide with pure sweet taste can be decomposed to obtain xylooligosaccharide, so that the overall taste and flavor can be better adjusted, and the sea cucumber is healthier.

3. The health care product has the advantages that the peach gum, the pummelo peel and the active substances in the sea cucumber are added to interact, so that the beauty and the face can be maintained, the excellent effects of resisting wrinkles and tendering skin are exerted, the skin is revived, the whole collocation of the health care product is simple, reasonable and mild, and the digestion and absorption are facilitated.

Drawings

FIG. 1 is a graph showing the results of measuring the degree of hydrolysis of sea cucumber protein according to the present invention.

Detailed Description

In order to clearly understand the technical solutions adopted by the present invention, the following description is made on the preferred embodiments of the present invention, and it should be understood that the embodiments described herein are only used for illustrating and explaining the present invention, and are not used to limit the present invention.

Example 1: a sea cucumber beauty health product comprises the following substances in parts by weight: 10.0 parts of sea cucumber, 5.0 parts of barley, 0.5 part of anthocyanin, 2.0 parts of peach gum, 3.0 parts of pummelo peel, 3.0 parts of brown rice and 8.0 parts of soda water.

And is prepared by the following preparation method:

s1, taking out viscera of fresh sea cucumbers, cleaning, putting the fresh sea cucumbers into a normal-temperature stirring pot, adding a proper amount of glacial acetic acid, starch and water into the stirring pot, soaking for 25min, then stirring at a low speed for 8min, taking out the sea cucumbers, and washing the sea cucumbers clean again for later use;

s2, grinding the sea cucumbers processed in the step S1 at a high speed to obtain sea cucumber slurry, putting the sea cucumber slurry into a sterile reverse reaction kettle, adding soda water and anthocyanin, stirring and uniformly mixing, transferring into a refrigerating chamber at the temperature of-5 ℃, standing and refrigerating for 2 hours, and transferring out of the refrigerating chamber for later use;

s3, adding xylanase and protease into the sea cucumber slurry mixture treated in the step S2, stirring, slowly heating to 44-45 ℃, preserving heat, continuously and slowly stirring, and carrying out enzymolysis reaction for 4 hours under the condition that the pressure is 60 KPa;

s4, respectively grinding the barley, the peach gum and the pummelo peel into powder for later use; parching brown rice with slow fire until the surface is yellowish, cooling, adding small amount of purified water, and grinding into brown rice pulp;

s5, adding the barley, peach gum, pummelo peel powder and brown rice pulp for later use one by one in a reaction kettle while stirring, keeping the temperature at 44-45 ℃, continuously and slowly stirring for reaction for 1 hour, adding the peach gum and pummelo peel which are ground into powder and purified water, stopping stirring, cooling to room temperature, homogenizing for 3-5min, discharging and filling to obtain a finished product 1.

Example 2: a sea cucumber beauty health product comprises the following substances in parts by weight: 25.0 parts of sea cucumber, 1.0 part of barley, 2.0 parts of anthocyanin, 2.0 parts of peach gum, 3.0 parts of pummelo peel, 3.0 parts of brown rice, 14.0 parts of soda water, 0.5 part of angelica, 0.5 part of lily, 0.5 part of medlar, 1.0 part of pawpaw and 1.0 part of dried longan.

And is prepared by the following preparation method:

s1, taking out viscera of fresh sea cucumbers, cleaning, putting the fresh sea cucumbers into a normal-temperature stirring pot, adding a proper amount of glacial acetic acid, starch and water into the stirring pot, soaking for 45min, then stirring at a low speed for 6min, taking out the sea cucumbers, and washing the sea cucumbers clean again for later use;

s2, grinding the sea cucumbers processed in the step S1 at a high speed to obtain sea cucumber slurry, putting the sea cucumber slurry into a sterile anti-reaction kettle, adding soda water and anthocyanin, stirring and uniformly mixing, transferring into a refrigerating chamber at 4 ℃, standing and refrigerating for 3 hours, and transferring out of the refrigerating chamber for later use;

s3, adding xylanase and protease into the sea cucumber slurry mixture treated in the step S2, stirring, slowly heating to 35-36 ℃, preserving heat, continuously and slowly stirring, and carrying out enzymolysis reaction for 5 hours under the condition that the pressure is 90 KPa;

s4, respectively grinding barley, peach gum, pummelo peel, angelica, lily, medlar, pawpaw and dried longan into powder for later use; parching brown rice with slow fire until the surface is yellowish, cooling, adding small amount of purified water, and grinding into brown rice pulp;

s5, adding the barley, peach gum, pummelo peel powder and brown rice pulp for later use into a reaction kettle while stirring, keeping the temperature at 45-36 ℃, continuously and slowly stirring for reaction for 1 hour, adding the peach gum, pummelo peel, angelica, lily, medlar, pawpaw and dried longan which are ground into powder, and purified water, stopping stirring, cooling to room temperature, homogenizing for 3-5min, discharging, adsorbing by activated carbon, filtering, and filling to obtain a finished product 2.

Example 3: a sea cucumber beauty health product comprises the following substances in parts by weight: 20.0 parts of sea cucumber, 3.0 parts of barley, 1.0 part of anthocyanin, 1.0 part of peach gum, 2.0 parts of pummelo peel, 2.0 parts of brown rice, 9.0 parts of soda water, 1.5 parts of angelica, 1.5 parts of lily, 1.0 part of medlar, 2.0 parts of pawpaw and 2.0 parts of dried longan.

And is prepared by the following preparation method:

s1, taking out viscera of fresh sea cucumbers, cleaning, putting the fresh sea cucumbers into a normal-temperature stirring pot, adding a proper amount of glacial acetic acid, starch and water into the stirring pot, soaking for 30min, then stirring at a low speed for 7min, taking out the sea cucumbers, and washing the sea cucumbers clean again for later use;

s2, grinding the sea cucumbers processed in the step S1 at a high speed to obtain sea cucumber slurry, putting the sea cucumber slurry into a sterile anti-reaction kettle, adding soda water and anthocyanin, stirring and uniformly mixing, transferring into a 0 ℃ refrigerating chamber, standing and refrigerating for 2.5 hours, and transferring out of the refrigerating chamber for later use;

s3, adding xylanase and protease into the sea cucumber slurry mixture treated in the step S2, stirring, slowly heating to 42 ℃, preserving heat, continuously and slowly stirring, and carrying out enzymolysis reaction for 4.5 hours under the condition that the pressure is 80 KPa;

s4, respectively grinding barley, peach gum, pummelo peel, angelica, lily, medlar, pawpaw and dried longan into powder for later use; parching brown rice with slow fire until the surface is yellowish, cooling, adding small amount of purified water, and grinding into brown rice pulp;

s5, adding the barley, peach gum, pummelo peel powder and brown rice pulp for later use into a reaction kettle while stirring, keeping the temperature at 42 ℃, continuously and slowly stirring for reaction for 1 hour, adding the peach gum, pummelo peel, angelica, lily, medlar, pawpaw and dried longan which are ground into powder, and purified water, stopping stirring, cooling to room temperature, homogenizing for 3-5min, discharging, adsorbing by activated carbon, filtering, and filling to obtain a finished product 3.

Wherein the adding ratio of the protease to the xylanase is 5: 1; the protease comprises mixed enzyme of subtilisin, trypsin and papain, and the addition ratio of the subtilisin, the trypsin and the papain is 2:1: 1.

Comparative example 1: the formula of the health product of example 3 and the corresponding preparation method were used to prepare a comparative product 1 without adding soda water.

Comparative example 2: the anthocyanin was not added, and the other ingredients were prepared using the formula of the health product of example 3 and the corresponding preparation method to give comparative product 2.

Comparative example 3: no xylanase is added, in order to keep the enzyme content consistent, the xylanase is replaced by protease, and the formula of the health care product in the embodiment 3 and the corresponding preparation method are adopted for preparation, so that a comparative finished product 3 is obtained.

Comparative example 4: the formula of the health product of example 3 and the corresponding preparation method were used to prepare a comparative product 4 without peach gum.

Comparative example 5: no exocarpium Citri Grandis was added, and the formula of the health product of example 3 and the corresponding preparation method were used to obtain comparative product 5.

Comparative example 6: no barley was added, the brown rice was used without being roasted, and the comparative product 5 was obtained by using the formula of the health food of example 3 and the corresponding preparation method.

Control group 1: the well-sold sea cucumber health care product purchased in the market is used as the product of the control group 1.

1. Long-term toxic and side effects tests were performed on the health products obtained in examples 1 to 3 and comparative examples 1 to 6 of the present invention.

Selecting 45 healthy rats with the same batch and the weight of 90 +/-10 g, randomly grouping the rats, wherein each group comprises 5 rats and 9 groups, feeding the same feed and water to all the rats tested in the whole experimental process, respectively carrying out tail vein injection administration on the rats of each group by using the finished products prepared in the examples 1-3 and the comparative examples 1-6, wherein the administration dose is 10ml/Kg, carrying out regular injection once a day, continuously administering for 3 months, measuring the weight of the rats every day, adjusting the administration dose according to the weight, and recording the health and development conditions of the rats.

After 2 months, the administration is stopped, the condition of the rats is recorded for 28 days, then 3 rats in each group are randomly selected and killed, blood is taken for measuring blood routine and blood biochemical indexes, internal tissues are planed, and the pathological histology of visceral organs of the planed rats is examined.

The test results show that: all rats tested were in stock, none died, and gained normal weight and developed normally, after 2 months of dosing and 28 days of drug withdrawal; the detection results of the blood routine and the blood biochemical index of each group of rats are normal; pathological histology examination is carried out on visceral organs of the debrided rat, and no pathological changes and abnormalities are found in brain, heart, liver, thymus, kidney, spleen, intestines and stomach and the like of the rat. The preparation has the advantages of mild components, no toxic or side effect after long-term use and good safety.

2. The hydrolysis degree of the sea cucumber protein is measured and tested: the Degree of hydrolysis of the sea cucumber proteins of examples 1 to 3 and comparative examples 1 to 6 was determined by trinitrobenzene sulfonic acid (TNBS), which is a method for determining the Degree of reliable hydrolysis of proteins, wherein the Degree of Hydrolysis (DH) of proteins is a percentage of peptide bonds in proteins that are hydrolyzed, and is calculated by using the following calculation formula:

DH% -the number of peptide bonds hydrolyzed/total number of peptide bonds in the protein X100%

＝h/h_tot×100％

Wherein h refers to the millimole number of peptide bonds (mmol/g protein) cleaved per gram protein after the hydrolysis of sea cucumber protein, and h refers to_totThe number of millimoles of peptide bonds in each gram of sea cucumber protein is shown, and when all the peptide bonds in the protein are hydrolyzed to generate free amino acids, the hydrolysis degree is 100 percent; when peptide bonds in the protein are not hydrolyzed, the degree of hydrolysis is 0. The final calculation and statistics are shown in fig. 1.

As can be seen from the results shown in fig. 1, the hydrolysis degree of the holothurian protein in the preparation methods of the holothurian health care products of examples 1 to 3 and comparative examples 4 to 6 is much higher than that in the preparation methods of the holothurian health care products of comparative examples 1 to 3, so that the method is proper in the process of processing holothurian, and the addition of soda water, anthocyanin and xylanase in the preparation process plays a critical role in the hydrolysis of holothurian protein, and the three are deficient and need to supplement each other, so that the efficient hydrolysis of holothurian protein can be realized in a relatively mild environment.

In particular, the preparation method of example 3, which has a degree of proteolysis as high as 74.4%, is also significantly higher than the preparation method of examples 1-2, and it can be seen that when the ratio of protease to xylanase is 5: 1; when the addition ratio of the subtilisin, the trypsin and the papain contained in the protease is 2:1:1, the effect of remarkably improving the degree of proteolysis can be achieved, which is also the best collocation and proportion discovered by the team of the invention through long-term research.

3. Test for improving immunocompetence: 55 mice of 35-40 g size purchased from the same batch are randomly divided into 11 groups of 5 mice each, and the mice are fed with the same feed in the same environment; wherein the health care products of examples 1-3, comparative examples 1-6 and control group 1 are respectively added into the drinking purified water of the mice of the 1 st to 10 th groups, and the adding amount is 5 percent; group 11 mice were fed for 25 days with purified water alone as drinking water.

The mouse eyeball blood sampling is carried out on each group of mice, the serum lysozyme content is determined, the agar plate with micrococcus and standard lysozyme with different concentrations are adopted, the mouse serum is diluted into samples with different concentrations, the samples are placed in a thermostat for 24 hours, the diameter of a lysozyme ring is measured, a standard curve is drawn, so that the lysozyme content of each serum sample is obtained, and the results are summarized as shown in the following table 2. The anticoagulation of heparin is adopted for each group of mice, the phagocytic function of white blood cells in blood is measured, the inactivated staphylococcus albus suspension is adopted for testing, the phagocytic speed and the phagocytic rate of the anticoagulation of the heparin of each group of mice are calculated, and the results are summarized as shown in the following table 2.

Table 2: results of animal immunocompetence determination

As is apparent from the results in table 2 above, the sea cucumber health products prepared in examples 1 to 3 have much more significant effects on enhancing the immunity, including increasing the content of serum lysozyme and increasing the phagocytic capacity of leukocytes, than the top-market sea cucumber health products purchased in control group 1, while the sea cucumber health products prepared in comparative examples 1 to 3 have much less effect on enhancing the immunity than the top-market sea cucumber health products prepared in examples 1 to 3, and even have less effect than the top-market sea cucumber health products purchased in control group 1. Therefore, the health care effects of the sea cucumber health care products obtained in the embodiments 1 to 3 are more comprehensive and efficient, and the hydrolysis degree of the sea cucumber protein and the retention degree of the active substances can be remarkably improved in the preparation processes of the embodiments 1 to 3, so that the overall efficiency is improved, therefore, the soda water, the anthocyanin and the xylanase added in the preparation processes not only play a critical role in the hydrolysis of the sea cucumber protein, but also can effectively avoid the loss or the loss of the active substances in the sea cucumber, so that the nutrient substances in the sea cucumber can be efficiently and fully utilized, and the effects of the sea cucumber health care products are improved.

4. And (3) taking effect test: after long-term toxic and side effects are completed and the results show safety and no toxic and side effects, and the health detection standards of food and drug products are passed, volunteer effect comparison tests are performed on the products of the above examples 1-3, comparative examples 1-6 and control group 1.

In the same area, 50 volunteers aged 35-55 years were collected and randomly divided into 10 groups of 5 persons, each volunteer had wrinkles of different depths on the face and had occasional insomnia symptoms, general mental state and no other serious diseases. The 10 groups of volunteers respectively drunk the health products of examples 1 to 3, comparative examples 1 to 6 and control group 1 once a day, 15ml each time, immediately stopped taking if any physical discomfort occurs in the period, and immediately hospitalized, each volunteer fed back the physical condition and the feeling after taking to the following person every day for 28 days, and finally summarized the test data and analyzed to obtain the test results as shown in the following table 3.

Table 3: test results of anti-wrinkle Effect

Table 4: test results of taste and gastrointestinal comfort

As is apparent from the test results in table 3 above, compared with the commercially available sea cucumber health products in the control group 1, the sea cucumber health products prepared in the embodiments 1-3 have excellent anti-wrinkle effects, and can significantly reduce or slowly lighten deep wrinkles on the face, and slowly improve skin vitality, while the sea cucumber health products prepared in the comparative examples 4-5 have much less anti-wrinkle and skin-tendering effects than the sea cucumber health products prepared in the embodiments 1-3, and the anti-wrinkle effects of the health products prepared in the comparative examples 1-3 are also significantly reduced, so that the synergistic effect of peach gum and pummelo peel can be seen, which has a critical effect in exerting excellent anti-wrinkle effects, and simultaneously needs to interact with active substances in the sea cucumber, and if the loss of the active substances in the sea cucumber is large, the anti-wrinkle effect can be influenced, only the active substances in the peach gum, the pummelo peel and the sea cucumber interact with each other to exert obvious effects of resisting wrinkles and tendering skin, so that the skin becomes young and active.

The test results in table 4 show that the top-selling sea cucumber health care product purchased in the market of the control group 1 is sweet and greasy in taste and is not suitable for the elderly to eat, even the diabetics eat with caution, while the sea cucumber health care products prepared in the comparative examples 3 and 6 are far from the taste of the sea cucumber health care products prepared in the embodiments 1 to 3, so that the mouth feeling of the xylanase, barley and brown rice can be obviously improved in the preparation process, the health care products are sweet but not sweet and greasy in taste, and the appetite of the health care products can be improved. While the sea cucumber health care products obtained in the examples 1 to 3 are mild and friendly to the intestines and the stomach, and the problems of dyspepsia or discomfort of the intestines and the stomach cannot occur, while the health care products in the comparative examples 1 to 3 and 6 and the control group 1 have certain stimulation to the intestines and the stomach and are not mild enough, so that the barley and the roasted brown rice are added, the full hydrolysis of the sea cucumber protein is ensured, the intestines and the stomach can be effectively protected, and the digestion and the absorption of the intestines and the stomach are facilitated.

In conclusion, the sea cucumber health care product prepared by the invention can fully hydrolyze sea cucumber protein in a relatively mild environment, simultaneously retain more active ingredients, has better health care effect, has the effects of beautifying and resisting wrinkles, enables skin to recover young vitality, is mild to intestines and stomach, does not generate toxic or side effect after being eaten for a long time, and ensures the ingredient safety.

The above description is only for the purpose of illustrating the embodiments of the present invention and not for the purpose of limiting the same, and equivalent modifications and variations of the embodiments of the present invention will be apparent to those skilled in the art without departing from the overall spirit of the invention.