阅读说明：本技术 用于选择性表征异生物质代谢中涉及的酶的探针以及制造和使用该探针的方法 (Probes for selective characterization of enzymes involved in xenobiotic metabolism and methods of making and using the same ) 是由 A·T·赖特 S·拉莫斯-亨特尔 C·惠德比 于 2018-10-03 设计创作，主要内容包括：描述了基于活性的探针,该探针可用于选择性鉴定和表征宿主及其微生物群中异生物质代谢不同阶段所涉及的酶。所描述的基于活性的探针仅特异性标记其参与异生物质代谢的靶活性酶,因此可提供真正蛋白质功能活性的量度,而不是转录本或蛋白质丰度的量度。基于活性的探针还提供了这些活性酶的多模态分析。还公开了用于制备基于活性的探针的方法和其使用的示例性方法。(Activity-based probes are described which can be used to selectively identify and characterize enzymes involved in different stages of xenobiotic metabolism in a host and its microbiota. The activity-based probes described specifically label only their target active enzymes involved in xenobiotic metabolism and thus can provide a measure of true protein functional activity, not transcript or protein abundance. Activity-based probes also provide multimodal analysis of these active enzymes. Also disclosed are methods for making activity-based probes and exemplary methods for their use.)