阅读说明：本技术 一种牛或羊脊骨骨髓肽的制备方法以及组合物 (Preparation method and composition of bovine or ovine spine bone marrow peptide ) 是由 宋根昌 于 2019-12-16 设计创作，主要内容包括：本发明属于骨髓肽制备技术领域,公开了一种牛或羊脊骨骨髓肽的制备方法以及组合物,制备方法包括步骤：冷冻粉碎、一次过滤、提取、酶解、二次过滤、浓缩和干燥制成牛或羊脊骨骨髓肽粉；提取步骤包括：将一次过滤得到的过滤液超声处理,得到牛或羊脊骨骨髓提取液；酶解步骤包括：向牛或羊脊骨骨髓提取液中加入骨胶原蛋白酶进行一次酶解,得到一次酶解液；调节一次酶解液的pH值为6~7,然后加入纤维蛋白酶,进行二次酶解,二次酶解结束后进行灭酶处理得到二次酶解液。本发明通过超声处理和三次酶解使得到的牛或羊脊骨骨髓肽,更容易吸收,品质更好；本发明的组合物对于治疗血虚、血瘀等气血虚弱的疾病具有较佳的疗效。(The invention belongs to the technical field of preparation of bone marrow peptide, and discloses a preparation method and a composition of bovine or ovine spine bone marrow peptide, wherein the preparation method comprises the following steps: freezing and crushing, filtering for the first time, extracting, performing enzymolysis, filtering for the second time, concentrating and drying to prepare the bovine or sheep spine bone marrow peptide powder; the extraction step comprises: carrying out ultrasonic treatment on the filtrate obtained by the primary filtration to obtain a cattle or sheep spine bone marrow extracting solution; the enzymolysis step comprises: adding bone collagenase into the cattle or sheep spine bone marrow extract for primary enzymolysis to obtain primary enzymolysis liquid; and adjusting the pH value of the primary enzymolysis liquid to 6-7, adding the fibrous protease to carry out secondary enzymolysis, and carrying out enzyme deactivation treatment after the secondary enzymolysis is finished to obtain secondary enzymolysis liquid. The obtained bovine or ovine spine bone marrow peptide is easier to absorb and has better quality through ultrasonic treatment and three times of enzymolysis; the composition has better curative effect on treating the diseases of deficiency of qi and blood such as blood deficiency, blood stasis and the like.)

1. A method for preparing bovine or ovine spine bone marrow peptide comprises the following steps: freezing and crushing, filtering for the first time, extracting, performing enzymolysis, filtering for the second time, concentrating and drying to prepare the bovine or sheep spine bone marrow peptide powder; the method is characterized in that:

the extraction step comprises: carrying out ultrasonic treatment on the filtrate obtained by primary filtration for 10-20 min at the temperature of 12-15 ℃ and the ultrasonic frequency of 400-2000 HZ to obtain a cattle or sheep spine bone marrow extracting solution;

the enzymolysis step comprises the following steps: adding bone collagenase into the cattle or sheep spine bone marrow extract for primary enzymolysis, wherein the primary enzymolysis temperature is 42-48 ℃, the primary enzymolysis time is 2.2-3 h, and the adding amount of the bone collagenase is 0.35-0.4% of the cattle or sheep spine bone marrow extract; after the primary enzymolysis treatment is finished, carrying out enzyme deactivation treatment to obtain primary enzymolysis liquid;

adjusting the pH value of the primary enzymolysis liquid to 6-7, and then adding the fibrous protease to carry out secondary enzymolysis; the secondary enzymolysis time is 1-2 h; the adding amount of the celloprotease is 0.35-0.4% of the bovine or ovine chine bone marrow extract, and after the secondary enzymolysis is finished, enzyme deactivation treatment is carried out to obtain secondary enzymolysis liquid.

2. The method for preparing bovine or ovine spine bone marrow peptide according to claim 1, wherein: the enzymolysis step further comprises: adjusting the pH value of the secondary enzymolysis liquid to 7-9, then adding trypsin for carrying out tertiary enzymolysis for 1-2 hours, wherein the adding amount of the trypsin is 0.35-0.4% of the bovine or sheep spine bone marrow extract, and carrying out enzyme deactivation treatment after the secondary enzymolysis is finished to obtain the tertiary enzymolysis liquid.

3. The method for preparing bovine or ovine spine bone marrow peptide according to claim 2, wherein: the step of freezing and crushing comprises the following steps: taking healthy cattle or sheep spines without meat and connective tissues, freezing the healthy cattle or sheep spines at-35 to-40 ℃ for 6 to 12 hours, removing the uncleaned connective tissues and fascia on the spines, and crushing to obtain the spine crushed material.

4. The method for preparing bovine or ovine spine bone marrow peptide according to claim 3, wherein: the primary filtration comprises the following steps: mixing the pulverized spine with pyrogen-free deionized water, stirring, and filtering to obtain filtrate.

5. The method for preparing bovine or ovine spine bone marrow peptide according to claim 4, wherein: the secondary filtration comprises the following steps: and filtering the three-time enzymolysis liquid obtained after enzymolysis through a barrel filter, a cotton cake filter and a multi-layer membrane filter with the pore diameter smaller than 0.1 mu m in sequence to obtain a secondary filtrate.

6. The method for preparing bovine or ovine spine bone marrow peptide according to claim 5, wherein: the concentration comprises the following steps: concentrating the secondary filtrate in an evaporation container until the solid content is 40-45% to obtain concentrated solution.

7. The method for preparing bovine or ovine spine bone marrow peptide according to claim 6, wherein: the drying comprises the following steps: and (4) carrying out spray drying on the concentrated solution to obtain the bovine or ovine chine bone marrow peptide powder.

8. A bovine or ovine spine bone marrow peptide composition characterized by: the composition comprises the following components in parts by weight: 5-15 parts of astragalus extract, 1-3 parts of angelica extract, 40-60 parts of concentrated whey protein, 15-25 parts of bovine or sheep bone marrow peptide obtained by the preparation method of any one of claims 1-7, 6-10 parts of ginseng extract and 8-12 parts of salvia extract.

9. The bovine or ovine spine bone marrow peptide composition of claim 8, wherein: the composition comprises the following components in parts by weight: 15 parts of astragalus extract, 3 parts of angelica extract, 60 parts of concentrated whey protein, 20 parts of bovine or sheep bone marrow peptide obtained by the preparation method of any one of claims 1 to 7, 7 parts of ginseng extract and 9 parts of salvia extract.

10. The bovine or ovine spine bone marrow peptide composition of claim 9, wherein: also comprises fructo-oligosaccharide with seasoning amount.

Technical Field

The invention belongs to the technical field of preparation of bone marrow peptides, and particularly relates to a preparation method and a composition of bovine or ovine spine bone marrow peptides.

Background

The bone marrow peptide is generally a polypeptide extracted from the bone marrow of animals such as cattle, sheep, etc. by an enzymolysis method. It is well known that bone marrow is not only a hematopoietic organ, but also an important immune organ. An important function of bone marrow is to produce stem cells that produce various cells, which are differentiated to regenerate various blood cells such as erythrocytes, leukocytes, platelets, lymphocytes, etc., and the bone marrow functions simply as hematopoiesis. Therefore, bone marrow plays a very important role in maintaining the life and immunity of the body. At present, the bone marrow peptide is mainly used for health care products and is used for realizing the health care effects of enriching blood and resisting aging.

Currently, the bone marrow peptide is generally prepared into polypeptide by an enzymolysis method for use, but the single enzymolysis method has higher production cost and low utilization rate of raw materials, and is easy to cause waste. And a composition for treating qi and blood deficiency diseases such as blood deficiency, blood stasis and the like by using the bone marrow peptide is lacked at present.

Disclosure of Invention

In order to solve the problems in the prior art, the invention aims to provide a preparation method and a composition of bovine or ovine spine bone marrow peptide.

On the one hand, the invention provides the following technical scheme:

a method for preparing bovine or ovine spine bone marrow peptide comprises the following steps: freezing and crushing, filtering for the first time, extracting, performing enzymolysis, filtering for the second time, concentrating and drying to prepare the bovine or sheep spine bone marrow peptide powder;

the extraction step comprises: carrying out ultrasonic treatment on the filtrate obtained by primary filtration for 10-20 min at the temperature of 12-15 ℃ and the ultrasonic frequency of 400-2000 HZ to obtain a cattle or sheep spine bone marrow extracting solution;

the enzymolysis step comprises the following steps: adding bone collagenase into the cattle or sheep spine bone marrow extract for primary enzymolysis, wherein the primary enzymolysis temperature is 42-48 ℃, the primary enzymolysis time is 2.2-3 h, and the adding amount of the bone collagenase is 0.35-0.4% of the cattle or sheep spine bone marrow extract; after the primary enzymolysis treatment is finished, carrying out enzyme deactivation treatment to obtain primary enzymolysis liquid;

adjusting the pH value of the primary enzymolysis liquid to 6-7, and then adding the fibrous protease to carry out secondary enzymolysis; the secondary enzymolysis time is 1-2 h; the adding amount of the celloprotease is 0.35-0.4% of the bovine or ovine chine bone marrow extract, and after the secondary enzymolysis is finished, enzyme deactivation treatment is carried out to obtain secondary enzymolysis liquid.

Further, the enzymolysis step further comprises: adjusting the pH value of the secondary enzymolysis liquid to 7-9, then adding trypsin for carrying out tertiary enzymolysis for 1-2 hours, wherein the adding amount of the trypsin is 0.35-0.4% of the bovine or sheep spine bone marrow extract, and carrying out enzyme deactivation treatment after the secondary enzymolysis is finished to obtain the tertiary enzymolysis liquid.

Further, the step of freeze-crushing comprises: taking healthy cattle or sheep spines without meat and connective tissues, freezing the healthy cattle or sheep spines at-35 to-40 ℃ for 6 to 12 hours, removing the uncleaned connective tissues and fascia on the spines, and crushing to obtain the spine crushed material.

Further, the primary filtration comprises the steps of: mixing the pulverized spine with pyrogen-free deionized water, stirring, and filtering to obtain filtrate.

Further, the secondary filtration comprises the steps of: and filtering the three-time enzymolysis liquid obtained after enzymolysis through a barrel filter, a cotton cake filter and a multi-layer membrane filter with the pore diameter smaller than 0.1 mu m in sequence to obtain a secondary filtrate.

Further, the concentrating comprises the steps of: concentrating the secondary filtrate in an evaporation container until the solid content is 40-45% to obtain concentrated solution.

Further, the drying includes the steps of: and (4) carrying out spray drying on the concentrated solution to obtain the bovine or ovine chine bone marrow peptide powder.

In another aspect, the present invention also provides a composition comprising:

the cattle or sheep spine bone marrow peptide composition comprises the following components in parts by weight: 5-15 parts of astragalus extract, 1-3 parts of angelica extract, 40-60 parts of concentrated whey protein, 15-25 parts of bovine or sheep bone marrow peptide, 6-10 parts of ginseng extract and 8-12 parts of salvia extract.

Further, the cattle or sheep spine bone marrow peptide composition comprises the following components in parts by weight: 15 parts of astragalus extract, 3 parts of angelica extract, 60 parts of concentrated whey protein, 20 parts of bovine or sheep bone marrow peptide, 7 parts of ginseng extract and 9 parts of salvia extract.

Furthermore, the fruit vinegar also comprises fructo-oligosaccharide with seasoning dosage.

The invention has the beneficial effects that: according to the preparation method and the composition of the bovine or sheep spine bone marrow peptide, the protein in the bone marrow cells of the bovine or sheep spine is extracted more thoroughly through ultrasonic treatment, the efficiency is higher, the waste of raw materials is avoided, and meanwhile, the three times of enzymolysis of the bone collagen protease, the fibrin protease and the trypsin are adopted for sequential treatment; protein in the bovine or ovine spinal bone marrow cells is degraded into bovine or ovine spinal bone marrow peptide with shorter peptide chain, thereby being more convenient to absorb; therefore, the obtained bovine or ovine spine bone marrow peptide is easier to absorb and has better quality through ultrasonic treatment and three times of enzymolysis; in addition, the composition utilizes the astragalus root and angelica sinensis blood replenishing soup, adds the red sage root for activating blood, and the ginseng for replenishing vital energy and supplements protein required by a human body through concentrated whey protein, thereby reducing the use of bovine or sheep spine bone marrow peptide, saving the cost and ensuring the use effect to the maximum extent, so the composition has better curative effect on diseases of deficiency of qi and blood such as blood deficiency, blood stasis and the like.

Detailed Description

The present invention is further illustrated below with reference to specific examples.