阅读说明：本技术 一种深海鱼小分子活性肽及其制备方法 (Deep sea fish small molecule active peptide and preparation method thereof ) 是由 路书刚 于 2019-12-07 设计创作，主要内容包括：本发明公开了一种深海鱼小分子活性肽及其制备方法,具体涉及生物肽领域,其中所使用的主料包括以下重量份数的原料：鲐鲅鱼25-32份、鳕鱼15-25份、秋刀鱼18-24份,所述辅料按重量百分比计包括0.1mol/L氢氧化钠30wt％-40wt％、0.2mol/L盐酸55wt％-65wt％、碱性蛋白酶1wt％、蛋白水解酶2wt％、胃蛋白酶2wt％。本发明通过以鲐鲅鱼、鳕鱼和秋刀鱼共同作为小分子活性肽的生产原料,因鲐鲅鱼和秋刀鱼较低的价格配合鳕鱼中的营养元素,可大大降低生产者的成本投入且不影响产品中的营养物质含量,且可降低传统产品中因原料产自海域而带有的次氯酸钠和氯化钠含量,从而保证本产品的食用口感,大大提高了本产品的适用范围。(The invention discloses a deep sea fish small molecule active peptide and a preparation method thereof, and particularly relates to the field of biological peptide, wherein the used main materials comprise the following raw materials in parts by weight: 25-32 parts of mackerel, 15-25 parts of cod and 18-24 parts of pacific saury, wherein the auxiliary materials comprise, by weight, 30-40% of 0.1mol/L sodium hydroxide, 55-65% of 0.2mol/L hydrochloric acid, 1% of alkaline protease, 2% of proteolytic enzyme and 2% of pepsin. The mackerel, cod and pacific saury are used as the production raw materials of the small molecule active peptide, and the lower price of the mackerel and pacific saury is matched with the nutrient elements in the cod, so that the cost investment of a producer can be greatly reduced, the content of nutrient substances in the product is not influenced, the content of sodium hypochlorite and sodium chloride in the traditional product caused by the raw materials produced from the sea can be reduced, the edible mouthfeel of the product is ensured, and the application range of the product is greatly improved.)

1. The deep sea fish small molecule active peptide is characterized in that: the used main materials comprise the following raw materials in parts by weight: 25-32 parts of mackerel, 15-25 parts of cod and 18-24 parts of pacific saury, wherein the auxiliary materials comprise, by weight, 30-40% of 0.1mol/L sodium hydroxide, 55-65% of 0.2mol/L hydrochloric acid, 1% of alkaline protease, 2% of proteolytic enzyme and 2% of pepsin.

2. The deep sea fish small molecule active peptide according to claim 1, is characterized in that: the used main materials comprise the following raw materials in parts by weight: 25 parts of mackerel, 25 parts of cod and 18 parts of pacific saury, wherein the auxiliary materials comprise, by weight, 30-40% of 0.1mol/L sodium hydroxide, 55-65% of 0.2mol/L hydrochloric acid, 1% of alkaline protease, 2% of proteolytic enzyme and 2% of pepsin.

3. The deep sea fish small molecule active peptide according to claim 1, is characterized in that: the used main materials comprise the following raw materials in parts by weight: 29 parts of mackerel, 20 parts of cod and 21 parts of pacific saury, wherein the auxiliary materials comprise, by weight, 30-40% of 0.1mol/L sodium hydroxide, 55-65% of 0.2mol/L hydrochloric acid, 1% of alkaline protease, 2% of proteolytic enzyme and 2% of pepsin.

4. The deep sea fish small molecule active peptide according to claim 1, is characterized in that: the used main materials comprise the following raw materials in parts by weight: 32 parts of mackerel, 15 parts of cod and 24 parts of pacific saury, wherein the auxiliary materials comprise, by weight, 30-40% of 0.1mol/L sodium hydroxide, 55-65% of 0.2mol/L hydrochloric acid, 1% of alkaline protease, 2% of proteolytic enzyme and 2% of pepsin.

5. The deep sea fish small molecule active peptide according to any one of claims 1 to 4, is characterized in that: the preparation method of the deep sea fish small molecule active peptide comprises the following specific preparation steps:

s1, preparing raw materials: preparing mackerel, cod and pacific saury according to a proportion, removing cheek and viscera, washing, soaking in blood, and draining;

s2, crushing raw materials: respectively cutting cleaned mackerel, cod and pacific saury in the step S1 into pieces, removing bones, and pulverizing the fish meat materials into muddy flesh materials;

s3, carrying out one-round enzymolysis: adding 0.1mol/L sodium hydroxide into the obtained meat paste raw material, then adding water to adjust the pH value of the solution to 7.5-9, heating the solution to 50-60 ℃ in a water bath heating mode, keeping the temperature, adding alkaline protease to carry out one-round enzymolysis, and stirring in the one-round enzymolysis process;

s4, carrying out two-round enzymolysis: cooling the first round of enzymolysis liquid to 35-40 ℃ and keeping, adding proteolytic enzyme for carrying out second round of enzymolysis, and stirring in the second round of enzymolysis process;

s5, three-cycle enzymolysis: adding 0.2mol/L hydrochloric acid into the second round of enzymolysis liquid to adjust the pH value of the solution to 4-6.5, then adding pepsin to carry out three-round enzymolysis, and stirring in the three-round enzymolysis process to obtain three rounds of enzymolysis liquid;

s6, filtering:

s6.1, filtering the three rounds of enzymatic hydrolysate obtained in the step S5 by using a gauze, and separating the incompletely decomposed fish meat and fish bones from the liquid to obtain a filtrate;

s6.2, filtering the filtrate obtained in the step S6.1 through a reverse osmosis membrane, and removing sodium chloride in the filtrate to obtain a clear solution;

s7, drying: and (4) concentrating the solution prepared in the step (S6) to thick slurry by adopting a water bath heating mode, and then carrying out spray drying to obtain the deep sea fish small molecule peptide dry product.

6. The preparation method of the deep sea fish small molecule active peptide according to claim 5, characterized in that: the stirring speed in the steps S3, S4 and S5 is set to 15-20 r/min.

7. The preparation method of the deep sea fish small molecule active peptide according to claim 5, characterized in that: the gauze in the step S6.1 is set to be a polyester gauze with 500 meshes and the aperture of 12-18 mu m, and the reverse osmosis membrane in the step S6.2 is set to be a reverse osmosis RO membrane.

8. The preparation method of the deep sea fish small molecule active peptide according to claim 5, characterized in that: the step S3 reaction time period is set to 60-75min, the step S4 reaction time period is set to 40-56min, and the step S5 reaction time period is set to 20-40 min.

Technical Field

The invention relates to the technical field of biological peptide, in particular to a deep sea fish small molecule active peptide and a preparation method thereof.

Background

Protein is one of eight essential nutrients, and the renewal, formation and repair of human body's cell tissue each day ensure the normal operation of body. Protein deficiency can lead to fatigue, emaciation, edema, and long-term deficiency can lead to illness. The protein is so important, but the protein absorbed by people in food every day is seriously insufficient, and modern science finds that 10 g of protein must be supplemented every day in addition to diet so as to guarantee the metabolism and physiological activities of human bodies. Therefore, in order to maintain health, the human body must be supplemented with sufficient protein. At present, the world recognizes that the supplementary protein is preferably supplemented in the form of small-molecule protein peptide.

The small molecule active peptide is a biochemical substance between amino acid and protein, has a molecular weight smaller than that of the protein and larger than that of the amino acid, and is a fragment of the protein. Two or more amino acids are linked by peptide bonds to form an "amino acid chain" or "amino acid string" called a peptide. Among them, peptides consisting of 10 to 15 or more amino acids are called polypeptides, peptides consisting of 2 to 9 amino acids are called oligopeptides, and peptides consisting of 2 to 15 amino acids are called small peptides or small peptides. In the digestive tract, peptides are better and faster in digestive absorption in the gastrointestinal tract than protein and free amino acid, and the peptides are shorter in retention time in the intestines and stomach than protein, have low frequency of gastroptosis and abdominal fullness, reduce the absorption area of the small intestine and damage the digestive function.

Disclosure of Invention

In order to overcome the above defects of the prior art, the embodiments of the present invention provide a deep sea fish small molecule active peptide and a preparation method thereof, and the technical problems to be solved by the present invention are: increasing the yield of small molecule active peptide prepared from deep sea fish and improving its taste.

In order to achieve the purpose, the invention provides the following technical scheme: the deep sea fish small molecule active peptide comprises the following main materials in parts by weight: 25-32 parts of mackerel, 15-25 parts of cod and 18-24 parts of pacific saury, wherein the auxiliary materials comprise, by weight, 30-40% of 0.1mol/L sodium hydroxide, 55-65% of 0.2mol/L hydrochloric acid, 1% of alkaline protease, 2% of proteolytic enzyme and 2% of pepsin.

In a preferred embodiment, the main materials used in the method comprise the following raw materials in parts by weight: 25 parts of mackerel, 25 parts of cod and 18 parts of pacific saury, wherein the auxiliary materials comprise, by weight, 30-40% of 0.1mol/L sodium hydroxide, 55-65% of 0.2mol/L hydrochloric acid, 1% of alkaline protease, 2% of proteolytic enzyme and 2% of pepsin.

In a preferred embodiment, the main materials used in the method comprise the following raw materials in parts by weight: 29 parts of mackerel, 20 parts of cod and 21 parts of pacific saury, wherein the auxiliary materials comprise, by weight, 30-40% of 0.1mol/L sodium hydroxide, 55-65% of 0.2mol/L hydrochloric acid, 1% of alkaline protease, 2% of proteolytic enzyme and 2% of pepsin.

In a preferred embodiment, the main materials used in the method comprise the following raw materials in parts by weight: 32 parts of mackerel, 15 parts of cod and 24 parts of pacific saury, wherein the auxiliary materials comprise, by weight, 30-40% of 0.1mol/L sodium hydroxide, 55-65% of 0.2mol/L hydrochloric acid, 1% of alkaline protease, 2% of proteolytic enzyme and 2% of pepsin.

The invention also comprises a preparation method of the deep sea fish small molecule active peptide, which comprises the following steps:

s1, preparing raw materials: preparing mackerel, cod and pacific saury according to a proportion, removing cheek and viscera, washing, soaking in blood, and draining;

s2, crushing raw materials: respectively cutting cleaned mackerel, cod and pacific saury in the step S1 into pieces, removing bones, and pulverizing the fish meat materials into muddy flesh materials;

s3, carrying out one-round enzymolysis: adding 0.1mol/L sodium hydroxide into the obtained meat paste raw material, then adding water to adjust the pH value of the solution to 7.5-9, heating the solution to 50-60 ℃ in a water bath heating mode, keeping the temperature, adding alkaline protease to carry out one-round enzymolysis, and stirring in the one-round enzymolysis process;

s4, carrying out two-round enzymolysis: cooling the first round of enzymolysis liquid to 35-40 ℃ and keeping, adding proteolytic enzyme for carrying out second round of enzymolysis, and stirring in the second round of enzymolysis process;

s5, three-cycle enzymolysis: adding 0.2mol/L hydrochloric acid into the second round of enzymolysis liquid to adjust the pH value of the solution to 4-6.5, then adding pepsin to carry out three-round enzymolysis, and stirring in the three-round enzymolysis process to obtain three rounds of enzymolysis liquid;

s6, filtering:

s6.1, filtering the three rounds of enzymatic hydrolysate obtained in the step S5 by using a gauze, and separating the incompletely decomposed fish meat and fish bones from the liquid to obtain a filtrate;

s6.2, filtering the filtrate obtained in the step S6.1 through a reverse osmosis membrane, and removing sodium chloride in the filtrate to obtain a clear solution;

s7, drying: and (4) concentrating the solution prepared in the step (S6) to thick slurry by adopting a water bath heating mode, and then carrying out spray drying to obtain the deep sea fish small molecule peptide dry product.

In a preferred embodiment, the stirring speed is set to 15 to 20r/min in the steps S3, S4 and S5.

In a preferred embodiment, the gauze in step S6.1 is a polyester gauze with a mesh size of 500 meshes and a pore size of 12-18 μm, and the reverse osmosis membrane in step S6.2 is a reverse osmosis RO membrane.

In a preferred embodiment, the step S3 reaction time period is set to 60-75min, the step S4 reaction time period is set to 40-56min, and the step S5 reaction time period is set to 20-40 min.

The invention has the technical effects and advantages that:

the mackerel, cod and pacific saury are used as the production raw materials of the small molecule active peptide, and the lower price of the mackerel and pacific saury is matched with the nutrient elements in the cod, so that the cost investment of a producer can be greatly reduced, the content of nutrient substances in the product is not influenced, the content of sodium hypochlorite and sodium chloride in the traditional product caused by the raw materials produced from the sea can be reduced, the edible mouthfeel of the product is ensured, and the application range of the product is greatly improved.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.