Intelligent seedling breeding system
阅读说明:本技术 一种智能化种苗繁育系统 (Intelligent seedling breeding system ) 是由 郑延海 王海兰 陈凯 陈傲然 于 2019-12-12 设计创作,主要内容包括:本发明公开了一种智能化种苗繁育系统,包括催芽罐、种子液输送管路、种子雨自动点播机和组培瓶,所述种子雨自动点播机包括机体,所述机体上设有滑轨,所述滑轨上滑动设有可移动播种探头,所述可移动播种探头沿轨道滑动,所述机体内还设有种子液暂存盒,所述组培瓶位于滑轨及播种探头下方,所述催芽罐通过种子液输送管路与种子液暂存盒连通,所述可移动播种探头相对滑轨上下运动,所述可移动播种探头在轨道上从种子液暂存盒上方滑动至组培瓶上方,所述可移动播种探头蘸取种子液暂存盒内的种子液并点播至组培瓶内。本发明的有益效果为工作效率高、污染少、生产成本低以及标准化。(The invention discloses an intelligent seedling breeding system which comprises a germination accelerating tank, a seed liquid conveying pipeline, an automatic seed rain dibbler and a tissue culture bottle, wherein the automatic seed rain dibbler comprises a machine body, a slide rail is arranged on the machine body, a movable seeding probe is arranged on the slide rail in a sliding manner and slides along the slide rail, a seed liquid temporary storage box is also arranged in the machine body, the tissue culture bottle is positioned below the slide rail and the seeding probe, the germination accelerating tank is communicated with the seed liquid temporary storage box through the seed liquid conveying pipeline, the movable seeding probe moves up and down relative to the slide rail, the movable seeding probe slides to the upper side of the tissue culture bottle from the upper side of the seed liquid temporary storage box on the slide rail, and the movable seeding probe dips in seed liquid in the seed liquid temporary storage box and dibbles the tissue culture bottle. The invention has the advantages of high working efficiency, less pollution, low production cost and standardization.)
1. An intelligent seedling breeding system is characterized in that: including vernalization jar, seed liquid pipeline, the automatic dibbler of seed rain and group's tissue culture bottle, the automatic dibbler of seed rain includes the organism, be equipped with the slide rail on the organism, it is equipped with portable seeding probe to slide on the slide rail, portable seeding probe slides along the track, still be equipped with the seed liquid box of keeping in the organism, group banks up the bottle with earth and is located slide rail and seeding probe below, the vernalization jar keeps in the box intercommunication through seed liquid pipeline and seed liquid, the relative slide rail up-and-down motion of portable seeding probe, portable seeding probe keeps in the box top from the seed liquid on the track and slides to group banks up bottle top with earth, portable seeding probe dips in the seed liquid of getting in the seed liquid box and dibbles to group and banks up in.
2. The intelligent seedling breeding system of claim 1, wherein: the germination accelerating tank is a closed seed suspension germination accelerating tank.
3. The intelligent seedling breeding system of claim 1, wherein: the seed liquid is kept in box top height and is less than germination accelerating tank top height, just seed liquid conveying pipeline stretches out from germination accelerating tank top, extends to the seed liquid and keeps in box top and insert the seed liquid box of keeping in downwards of keeping in the seed liquid along the horizontal direction. The seed liquid conveying pipeline has a design structure, so that when the liquid level in the box is lower than a certain height, the seed suspension liquid is automatically supplemented from the closed seed suspension liquid germination accelerating tank under the action of atmospheric pressure.
4. The intelligent seedling breeding system of claim 1, wherein: the movable seeding probe is arranged on the machine body and drives the movable seeding probe to slide along the length direction of the sliding rail.
5. The intelligent seedling breeding system of claim 1, wherein: the movable seeding probe is arranged on the machine body and drives the movable seeding probe to move up and down relative to the sliding rail.
6. The intelligent seedling breeding system of claim 1, wherein: still include a conveyer belt, the conveyer belt sets up in track and portable seeding probe below for carry group and organize the bottle with earth.
7. The intelligent seedling breeding system of claim 1, wherein: still include a group and organize the basket, group banks up the tissue culture bottle setting on group banks up the basket with earth, group banks up the tissue culture basket bottom and is equipped with the frictional layer of reinforcing and conveyer belt frictional force.
8. The intelligent seedling breeding system of claim 1, wherein: still include the bottle lid machine, the bottle lid machine sets up the end at conveyer belt direction of transfer, the bottle lid machine will organize group the group when the bottle conveys the end with group for group banks up the bottle lid upper cover with earth at the conveyer belt.
9. The intelligent seedling breeding system of claim 1, wherein: the germination accelerating tank is communicated with the oxygenation device through an oxygen conveying pipeline and comprises a temperature control device, and the temperature control device is arranged at the bottom of the germination accelerating tank and used for adjusting the temperature of the planting suspension.
10. The intelligent seedling breeding system of claim 1, wherein: the seed rain automatic dibbler also comprises a seed liquid volume quantitative setting controller, so as to control the volume of the liquid drops dibbled onto the culture medium by the movable sowing probe according to the concentration of the seed suspension and the seed number demand in the liquid drops.
Technical Field
The invention relates to the field of agricultural planting, in particular to an intelligent seedling breeding system.
Background
With the continuous development of scientific technology, more and more automatic technologies are applied to the field of agricultural planting, and the technologies are used for reducing the labor cost required for planting crops, improving the working efficiency and reducing the production cost. The plant tissue culture technology is a new traditional Chinese medicine seedling rapid propagation technology developed in recent years, is a technology for obtaining a regenerated complete plant by inoculating tissues, organs and the like of a plant on a culture medium containing various nutrient substances and phytohormones under an aseptic condition through aseptic operation, and has the characteristics of high propagation efficiency, short period and regular and consistent seedlings. The prior Chinese medicinal material tissue culture and rapid propagation technology has the following problems: 1) the tissue culture seedling inoculation equipment is simple, the system integration level is low, the compactness is poor, a large number of high-quality technical workers are needed in each link, the inoculation quality and the inoculation speed are guaranteed, and the labor cost is high; 2) the seedling raising system has high frame manufacturing cost and very low intellectualization degree, is difficult to form a seedling raising and planting system with unified standard, all links also need manual control, and the quality of inoculated seedlings is uneven due to different technical proficiency degrees of operators. 3) The operation link is complicated and inconvenient, the production efficiency is low, and the production cost is high.
Disclosure of Invention
Based on the defects in the prior art, the invention provides an intelligent seedling breeding system. Through improving the structure of whole group banks up the system with earth, adopt the liquid of innovation to carry and the dibble seeding mode, realize the accurate supply of the nutrient solution of growing seedlings, greatly improved the efficiency of growing seedlings to resource saving has been played, pollution abatement's beneficial effect.
In order to achieve the purpose, the invention adopts the technical scheme that: the utility model provides an intelligent seedling breeding system, includes germination accelerating tank, seed liquid pipeline, the automatic dibbler of seed rain and group banks up the bottle with earth, the automatic dibbler of seed rain includes the organism, be equipped with the slide rail on the organism, it is equipped with portable seeding probe to slide on the slide rail, portable seeding probe slides along the track, still be equipped with the seed liquid box of keeping in the organism, the group banks up the bottle with earth and is located slide rail and seeding probe below, germination accelerating tank passes through seed liquid pipeline and seed liquid box intercommunication of keeping in, the relative slide rail up-and-down motion of portable seeding probe, portable seeding probe slides to group banks up the bottle top from seed liquid box top of keeping in on the track, portable seeding probe dips in the seed liquid box of keeping in and evenly dibbles to group banks up the bottle with earth with the.
Further, the germination accelerating tank is a closed seed suspension germination accelerating tank.
Further, the seed liquid is kept in box top height and is less than germination accelerating tank top height in the seed liquid, just seed liquid conveying pipeline stretches out from germination accelerating tank top, extends to the seed liquid and keeps in box top and insert in the seed liquid box of keeping in downwards along the horizontal direction. The seed liquid conveying pipeline has a design structure, so that when the liquid level in the box is lower than a certain height, the seed suspension liquid is automatically supplemented from the closed seed suspension liquid germination accelerating tank under the action of atmospheric pressure.
Further comprises a first driving device which is arranged on the machine body and drives the movable sowing probe to slide along the length direction of the slide rail,
and the movable seeding probe is driven to move up and down relative to the sliding rail by the second driving device arranged on the machine body.
Further, still include a conveyer belt, the conveyer belt sets up in track and portable seeding probe below for carry group to organize the bottle.
Furthermore, the tissue culture bottle is arranged on the tissue culture basket, and a friction layer for enhancing the friction force between the tissue culture bottle and the conveyor belt is arranged at the bottom of the tissue culture basket. A limiting mechanism used for limiting the position of the tissue culture bottle is arranged in the tissue culture basket.
Further, still include bottle capper, the bottle capper sets up the end at conveyer belt direction of transfer, the bottle capper is group banks up the lid for group when the conveyer belt will bank up the group with the group the bottle and convey the end.
Furthermore, a culture medium is arranged in the tissue culture bottle.
Further, the germination accelerating tank is communicated with the oxygen adding device through an oxygen conveying pipeline. And adjusting the dissolved oxygen of the seed suspension in the germination accelerating tank by an oxygen adding device.
Further, the device comprises a temperature control device, wherein the temperature control device is arranged at the bottom of the germination accelerating tank and is used for adjusting the temperature of the planting suspension. The temperature control device is specifically an electromagnetic heating device or an electric heating wire heater.
Furthermore, the seed rain automatic dibbler also comprises a seed liquid volume quantitative setting controller, so as to control the volume of the liquid drops dibbled onto the culture medium by the movable sowing probe according to the concentration of the seed suspension and the seed number demand in the liquid drops.
The invention also discloses an intelligent seedling breeding method which is realized by adopting the intelligent seedling breeding system and comprises the following steps:
(1) preparing a seed suspension with specific seed density and specific agar concentration;
(2) loading the seed suspension into a closed seed suspension germination accelerating tank;
(3) uniformly stirring the seed suspension in the closed seed suspension germination accelerating tank by using a stirrer;
(4) the seed suspension liquid which is evenly stirred is conveyed to a seed liquid temporary storage box by a seed liquid conveying pipeline, a movable seeding probe on the automatic seed rain dibbler slides along a slide rail under the action of a driving device, the movable seeding probe slides to the upper part of the seed liquid temporary storage box, and then the seed suspension liquid in the seed liquid temporary storage box is dipped by downward movement under the action of the driving device,
(5) the movable sowing probe moves to the upper part of the tissue culture bottle under the action of the driving device, and the seed suspension is evenly dibbled to a culture medium in the tissue culture bottle in a seed rain mode.
And (3) further conveying the tissue culture bottles after the seeds are sown in the step (6) to a bottle covering machine through a conveying belt, covering the bottle covers and placing the bottle covers on a culture rack with a proper light source.
Furthermore, the density range of the specific seeds is 100-3000 grains/mL, and preferably 150-250 grains/mL.
Furthermore, the concentration range of the specific agar is 1-10 g/L, which refers to the mass of the agar in a unit volume of solution.
Further, in the step (2), the temperature in the germination accelerating tank is controlled to be 23-26 ℃, and the oxygen content is controlled to be 5-8 mg/l.
Further, in the step (4), when the seeds exposed to the white in the germination accelerating tank are conveyed to the seed liquid temporary storage box through the seed liquid conveying pipeline, the seed suspension liquid which is uniformly stirred is conveyed to the seed liquid temporary storage box.
Further, in the step (5), the seeds are sown in a seed rain mode by controlling the density of the liquid drops to be 0.15-0.25 drops/cm 2 and the number of the seeds per drop to be 2-3.
The invention has the beneficial effects that:
the application of the intelligent seedling raising system reduces the operation links, and has the advantages of high working efficiency, less pollution, low production cost and standardization.
The invention replaces the full-artificial inoculation seedling breeding technology with intelligent equipment, greatly reduces the labor cost and improves the seedling quality consistency through standardized operation.
The tissue culture container used in the invention is a cylindrical bottle, the diameter of the upper cover is slightly larger than that of the lower body, so that the tissue culture container can be tightly covered and sealed, and the upper cover is made of a material with high light transmittance, so that the waste of light sources can be reduced to the maximum extent, and the light energy utilization rate and the growth rate of tissue culture seedlings can be improved.
Drawings
Fig. 1 is a schematic diagram of the intelligent seedling breeding system of this embodiment.
FIG. 2 is a schematic structural view of the seed rain automatic dibbler of the present embodiment.
Fig. 3 is a schematic structural diagram of the first driving device of the present embodiment.
Fig. 4 is a schematic structural diagram of a second driving device according to the present embodiment.
Description of reference numerals: 1. temperature control device, 2, vernalization jar, 3, agitator, 4, oxygenation device, 5, seed liquid conveying line, 6, oxygen conveying line, 7, the automatic dibbler organism of seed rain, 8, the seed liquid box of keeping in, 9, slide rail, 10, portable seeding probe, 11, group banks up the bottle with earth, 12 group banks up the basket with earth, 13, conveyer belt, 14, bottle lid machine, 15, bottle lid, 16, first drive arrangement, 17, second drive arrangement.
Detailed Description
The invention is further described with reference to the following drawings and detailed description.
An intelligent seedling breeding system is shown in fig. 1 and fig. 2. The utility model provides an intelligent seedling breeding system, includes temperature control device 1, vernalization jar 2 is closed seed suspension vernalization jar 2, and seed
The germination accelerating tank 2 is communicated with the oxygen adding device 4 through an oxygen conveying pipeline 6. The dissolved oxygen of the seed suspension in the germination accelerating tank 2 is adjusted by an oxygen adding device.
Still be equipped with seed liquid in the organism 7 and keep in
The
The
The temperature control device is arranged at the bottom of the germination accelerating tank 2 and is used for adjusting the temperature of the planting suspension. The temperature control device 1 is specifically an electromagnetic heating device or a heating wire heater, or the temperature control device 1 adopts a temperature sensor, an MCU and an electromagnetic heating device, the temperature sensor collects temperature information and sends the temperature information to the MCU, the MCU generates a control signal and sends the control signal to the electromagnetic heating device, and the electromagnetic heating device receives the control signal to start or close an electromagnetic heating function and control electromagnetic heating time.
The seed rain automatic dibbler also comprises a seed liquid quantitative setting controller, so as to control the volume of the liquid drops dibbled onto the culture medium by the movable sowing probe according to the concentration of the seed suspension and the seed number requirement in the liquid drops. The volume setting method comprises the following steps: taking dendrobium as an example, 20 ten thousand dendrobium seeds are added into 1L of liquid with agar concentration of 1-3 g/L, namely, a uniform seed suspension with concentration of 0.2 grains/microliter is generated. Then, the movable sowing probe is controlled to dibble the liquid drop on the culture medium with a volume of 10 microliter according to the concentration of the seed suspension and the seed number requirement (e.g. 2 grains/drop) in the liquid drop.
According to the device provided by the embodiment of the invention, the closed seed suspension germination accelerating tank is arranged, so that the optimum condition for seed germination accelerating is perfectly realized, the germination vigor and consistency are improved, the cleanliness of the inner space of the germination tank is effectively ensured, and the pollution is reduced. The homogeneity of the seed suspension is achieved by adjusting the agar content of the liquid, which ensures that a relatively consistent "seed rain" is obtained. Oxygen adding pump for ensuring proper concentration of O in seed suspension2The content can provide enough oxygen required by seed germination, thereby improving the germination vigor and the germination rate to the maximum extent and optimizing the quality of a seed suspension.
The embodiment of the invention utilizes the characteristic that partial Chinese medicinal material seeds can be subjected to suspension liquid germination acceleration, obtains the suspension liquid with uniformly distributed seeds by adjusting the agar concentration of the suspension liquid and properly stirring, uniformly seeds the suspension liquid with germination acceleration and white exposure to a culture medium in tissue culture bottles through intelligent seed rain dibbling equipment, accurately controls the density of liquid drops, and basically keeps the number of seeds in each liquid drop consistent, so that the number of seeds in each tissue culture bottle is basically consistent, the sowed tissue culture bottle is covered and placed on a culture frame, and high-quality seedlings are obtained through meticulous culture for a certain time.
By adopting the technical scheme in the embodiment, the following technical effects are achieved:
1) the seed germination accelerating device is relatively closed, is clean and environment-friendly, and effectively avoids germ pollution;
2) the buoyancy of the seed suspension is adjusted by changing the agar concentration of the solution, and then the uniform distribution of the seeds in the solution is realized by stirring;
3) the intelligent seed rain dibbler realizes the uniform distribution of seed liquid drops and the basic consistency of the number of seeds in each tissue culture bottle;
4) the transmission frequency of the tissue culture bottle after seeding is precisely consistent with the working frequency of the bottle capping machine, so that the two devices are well matched;
5) the tissue culture bottle covered with the cover is placed on a clean culture room culture shelf, and strong, tidy and consistent and pollution-free excellent seedlings are cultured through careful management and culture.
The embodiment of the invention also discloses an intelligent seedling breeding method which is realized by adopting the intelligent seedling breeding system and comprises the following steps:
(1) preparing a seed suspension with specific seed density and specific agar concentration; the specific seed density range is preferably 150-250 grains/ml in the embodiment. The concentration range of the specific agar is 1-5 g/L.
(2) The seed suspension is put into a closed seed suspension germination accelerating tank 2; controlling the temperature in the germination accelerating tank to be 23-26 ℃, and the oxygen content to be 5-8 mg/L.
(3) Uniformly stirring the seed suspension in the closed seed suspension germination accelerating tank by using a
(4) carry the seed suspension with 5 ways of seed liquid conveyer pipe with the stirring to the
(5) The movable sowing probe moves to the upper part of the
And (6) conveying the
The following description further illustrates the process and beneficial effects of the intelligent seedling breeding method according to the embodiment, with respect to a specific application embodiment of seeding and cultivating dendrobe fruit pods. The method specifically comprises the following steps:
1) the preferable selection of the dendrobium fruit pods: selecting Dendrobium huoshanense fruit pods with relatively consistent individual size and maturity (aiming at ensuring relatively consistent seed quantity, germination vigor and germination rate of Dendrobium huoshanense), and performing shell alcohol sterilization to break the pods for preparing seed suspension.
2) Determination of the seed suspension concentration: according to the germination rate and the sowing density of the dendrobium huoshanense seeds, the number of the seeds in the seed liquid per unit volume is determined to be 150-250 seeds/mL.
2) And (3) blending uniformity of the seed suspension: according to the weight and the suspension force of the dendrobium huoshanense seeds, a method of increasing the concentration (1-3 g/L) of agar in the solution to improve the suspension force and fully stirring is adopted to obtain a uniform dendrobium huoshanense seed suspension.
3) Putting the prepared seed suspension into a translucent closed germination accelerating tank which is wiped clean in advance, and uniformly stirring at the temperature of 23-26 ℃ and the oxygen content of 5-8 mg/L;
4) seeds exposed to the white in the germination accelerating tank (7-10 days) are separately planted on a culture medium in a tissue culture bottle through an intelligent seed rain dibbler, the density of liquid drops is 0.15-0.25 drops/cm 2, and the number of the seeds per drop is 2-3;
5) conveying the tissue culture bottles after the seeds are sown to a bottle covering machine through a conveying belt, covering the bottle covers and placing the bottle covers on a culture rack with a proper light source;
6) the illumination intensity in the whole growth period is maintained at 50-70 mu mol m < -2 > s < -1 >, the illumination time is 8-16 hours, the ambient temperature is 22-26 ℃, and the ambient humidity is 45-65%;
7) and carefully culturing for 180-210 days to obtain the high-quality dendrobium huoshanense seedlings.
The method embodiment of the invention adopts the traditional Chinese medicine seed suspension liquid culture germination accelerating technology, selects the seeds with basically consistent size, germination vigor and germination rate, adopts a closed semi-transparent seed liquid germination accelerating tank, realizes the neat and consistent germination of the seeds through the control of temperature and a dissolved oxygen system, and adjusts the buoyancy of the solution through adjusting the concentration of agar in the solution, thereby obtaining the uniformly distributed seed suspension liquid;
the invention adopts an intelligent seed rain dibbling technology, in the invention, seed liquid is dibbled to a culture medium in a tissue culture bottle through a movable seed rain dibbling disk, the volume of each liquid drop is determined according to the seed concentration of a suspension and the seed quantity requirement in each liquid drop, and the number of the seeds dibbled to each tissue culture bottle can be ensured to be basically consistent;
the invention breaks through the traditional culture method of 3 sections (germination, proliferation and rooting) of the tissue culture seedlings of the traditional Chinese medicinal materials, combines the proliferation stage and the rooting stage for culture, reduces the working links, shortens the culture period and saves the production cost.
Although the embodiments have been described, once the basic inventive concept is obtained, other variations and modifications of these embodiments can be made by those skilled in the art, so that the above embodiments are only examples of the present invention, and not intended to limit the scope of the present invention, and all equivalent structures or equivalent processes using the contents of the present specification and drawings, or any other related technical fields, which are directly or indirectly applied thereto, are included in the scope of the present invention.
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