阅读说明：本技术 一种纳米银/聚多巴胺/聚丙烯复合补片材料的制备方法 (Preparation method of nano-silver/polydopamine/polypropylene composite patch material ) 是由 张振东 于 2019-10-21 设计创作，主要内容包括：本发明涉及医学材料领域,具体地,涉及一种纳米银/聚多巴胺/聚丙烯复合补片材料的制备方法。利用聚多巴胺黏附性、亲水性和生物相容性好的特性,将多巴胺氧化聚合成聚多巴胺包覆在聚丙烯网片的表面,降低由聚丙烯接触腹腔脏器所引起的肠粘连等并发症的风险。聚多巴胺对金属离子具有螯合和还原特性,银离子被还原成纳米银沉积于聚多巴胺膜的表面,获得抗菌性。采取的技术方案包括如下步骤：S1、将聚丙烯网片置于多巴胺溶液中进行反应,形成聚多巴胺膜包覆在聚丙烯网片表面,得到聚多巴胺/聚丙烯复合网片；S2、将所述聚多巴胺/聚丙烯复合网片材料置于硝酸银溶液中进行反应,纳米银沉积于聚多巴胺膜表面,得到纳米银/聚多巴胺/聚丙烯复合补片材料,仍然保持有原来的网孔结构,利于组织长入、巨噬细胞和白细胞的自由进出。制作方法简单,成本低。(The invention relates to the field of medical materials, in particular to a preparation method of a nano-silver/polydopamine/polypropylene composite patch material. By utilizing the characteristics of good adhesion, hydrophilicity and biocompatibility of the polydopamine, the dopamine is oxidized and polymerized into the polydopamine to be coated on the surface of the polypropylene mesh, so that the risk of complications such as intestinal adhesion and the like caused by the contact of the polypropylene with organs in the abdominal cavity is reduced. The polydopamine has chelating and reducing characteristics to metal ions, and silver ions are reduced into nano silver to be deposited on the surface of the polydopamine membrane, so that the antibacterial property is obtained. The technical scheme adopted comprises the following steps: s1, placing the polypropylene mesh into a dopamine solution for reaction to form a polydopamine film to coat the surface of the polypropylene mesh so as to obtain a polydopamine/polypropylene composite mesh; s2, placing the polydopamine/polypropylene composite mesh sheet material in a silver nitrate solution for reaction, and depositing nano silver on the surface of a polydopamine membrane to obtain the nano silver/polydopamine/polypropylene composite patch material which still maintains the original mesh structure and is beneficial to tissue ingrowth and free access of macrophages and leukocytes. The manufacturing method is simple and the cost is low.)

1. A preparation method of a nano-silver/polydopamine/polypropylene composite patch material is characterized by comprising the following steps:

s1, placing the polypropylene mesh into a dopamine solution for reaction to form a polydopamine film to coat the surface of the polypropylene mesh, so as to obtain a polydopamine/polypropylene composite mesh;

s2, placing the polydopamine/polypropylene composite mesh into a silver nitrate solution for reaction, and depositing nano silver on the surface of the polydopamine film to obtain the nano silver/polydopamine/polypropylene composite patch material.

2. The method for preparing a nano silver/poly dopamine/polypropylene composite patch material according to claim 1, wherein the dopamine solution in step S1 is prepared according to the following method: taking 10mM Tris-HCl buffer solution, adjusting the pH value to 8.5 by using 0.5mol/L sodium hydroxide solution, adding dopamine, uniformly mixing and stirring to obtain dopamine solution.

3. The preparation method of the nano-silver/poly-dopamine/polypropylene composite patch material according to claim 1, wherein the concentration of the dopamine solution in step S1 is 0.4-2 g/L.

4. The preparation method of the nano-silver/poly-dopamine/polypropylene composite patch material according to claim 1, wherein the reaction time in step S1 is 8-24 hours.

5. The method for preparing a nano silver/poly dopamine/polypropylene composite patch material according to claim 1, wherein the concentration of the silver nitrate solution in the step S2 is 20 mM.

6. The preparation method of the nano-silver/poly-dopamine/polypropylene composite patch material according to claim 1, wherein the reaction time in step S2 is 1-4 hours.

Technical Field

The invention relates to the field of medical materials, in particular to a preparation method of a nano-silver/polydopamine/polypropylene composite patch material.

Background

Tension-free repair surgery is the mainstream of hernia treatment nowadays, and the number of patches used is increasing year by year. However, related complications emerge and are increasingly appreciated. Patch infection is one of the more serious complications and is very tricky to handle. If the patch is taken out in the operation, the problems of high operation difficulty, large wound, easy side injury of organs such as an intestinal canal, a spermatic cord, a vas deferens, a bladder and the like, hernia recurrence and the like can be faced; if the infection is treated by conventional dressing change drainage and antibiotics, the infection may not be cured, and the possibility of long hospitalization time, high medical cost, repeated and worsened disease of patients is faced. Thus, infection of the patch is a difficult problem for surgeons!

The reasons for the infection of the patch are complex, and are related to the bacteria-carrying state of the operation area, the constitution and basic diseases of the patient, the physicochemical properties of the artificial patch material and the like besides some well-known reasons (such as irregular skin cleaning in the operation area, irregular operation in the operation and irregular use of antibiotics in the perioperative period).

The hernia repair patch commonly used at present is a polypropylene substrate, a polyester patch and an expanded polytetrafluoroethylene patch. Polypropylene patches have had a tremendous impact on the surgical field over the past 50 years, and have proven to be the most popular implant in the surgical field today. But it can not directly contact the viscera in the abdominal cavity, otherwise it can produce serious adhesion, and then cause intestinal obstruction, intestinal fistula and even serious abdominal infection.

Chinese patent application No. CN201811559554.X discloses an antibacterial and anti-inflammatory hernia repair patch and a preparation method thereof, wherein a high polymer film layer made of a degradable high polymer material and a biocompatible high polymer material composite solution is arranged outside a polypropylene-based patch. The biocompatible polymer material composite solution is composed of chitosan, sodium alginate and antibacterial peptide according to a certain weight ratio. Because the patch is formed by hot pressing a substrate and a polymer film, the patch is easy to fall off and separate, is fast to degrade and short in duration, the research on the antibacterial peptide is mainly focused on a laboratory and an in-vitro test at present, and if the antibacterial peptide is put into the market, a series of problems of source, safety, activity, stability and the like need to be solved in the medical aspect.

Chinese patent CN201410124093.9 provides an anti-infection hernia repair patch containing nano-silver and a preparation method thereof, wherein the nano-silver stabilized by high molecular polymer is fixedly carried on the surface of the base material of the hernia repair patch by coating, dipping, spin coating, spraying or spraying. However, the nano silver is released in a short time, the duration is only a few days, and the long-acting antibacterial ability is not realized.

The biological characteristics of the nano silver are shown as follows: 1) is antibacterial. Nanosilver is a broad-spectrum bactericidal agent against gram-positive and gram-negative bacteria, including antibiotic-resistant strains. 2) And (4) antifungal property. Nano-silver is also a broad-spectrum antifungal agent, including aspergillus and saccharomyces fungi, among others. 3) Resisting virus. 4) Anti-inflammatory. The proteolytic enzyme is an important enzyme in inflammatory reaction and tissue repair process, and the nano silver can influence the expression of the proteolytic enzyme so as to inhibit the inflammatory reaction. 5) Inhibiting biofilm formation. The biofilm forms a barrier to bacteria and prevents antibacterial agents from coming into contact with the bacteria, resulting in persistent infection at the site where the biofilm is formed. The nano silver can inhibit the formation of a biological film, thereby preventing persistent infection. The excellent sterilizing ability and infection treating ability of the nano-silver have remarkable effects in the application aspects of the related fields of catheters, limb prostheses, dentistry and the like. The low-content nano silver can kill bacteria before the bacteria are adhered and form a biological film, and can not generate drug resistance and has long-term effect. However, research shows that the nano-silver can enter a human body through skin, breath, oral administration and other ways, and participate in blood circulation to reach organs such as heart, kidney, liver, spleen, lung and the like of the human body, normal cells of the human body contacted with the nano-silver can be damaged due to a special antibacterial mechanism of the nano-silver, no conclusion about the consistent usage amount of the nano-silver exists, and the controllable preparation of the nano-silver has important significance on biological safety and clinical application.

Disclosure of Invention

The invention aims to overcome the problems and provides a preparation method of a nano-silver/polydopamine/polypropylene composite patch material, so that the traditional polypropylene patch can achieve the double effects of antibiosis and anti-adhesion through surface modification.

The technical scheme adopted by the invention comprises the following steps:

s1, placing the polypropylene mesh into a dopamine solution for reaction to form a polydopamine film to coat the surface of the polypropylene mesh, so as to obtain a polydopamine/polypropylene composite mesh;

s2, placing the polydopamine/polypropylene composite mesh sheet material in a silver nitrate solution for reaction, and depositing nano silver on the surface of the polydopamine film to obtain the nano silver/polydopamine/polypropylene composite patch material.

The dopamine solution in step S1 is prepared according to the following method: taking 10mM Tris-HCl buffer solution, adjusting the pH value to 8.5 by using 0.5mol/L sodium hydroxide solution, adding dopamine, uniformly mixing and stirring to obtain dopamine solution, controlling the concentration of the dopamine solution to be 0.4-2 g/L, and reacting for 8-24 h.

In the step S2, the concentration of the silver nitrate solution is 20mM, and the reaction time is 1-4 h.

The invention has the beneficial effects that:

by utilizing the characteristics of good adhesion, hydrophilicity and biocompatibility of the polydopamine, the dopamine is oxidized and polymerized into the polydopamine to be coated on the surface of the polypropylene mesh, so that the polypropylene material does not directly contact abdominal organs, and the risk of complications such as intestinal adhesion and the like caused by the polypropylene contacting the abdominal organs can be reduced.

The polydopamine has chelating and reducing characteristics to metal ions, and silver ions are reduced into nano silver to be deposited on the surface of the polydopamine membrane, so that the antibacterial property is obtained.

3. The biological safety risk can be minimized while the antibacterial performance is obtained by controlling the proper nano silver deposition time.

4. The antibacterial polydopamine/polypropylene composite patch material still maintains the original mesh structure and is beneficial to tissue ingrowth and free access of macrophages and leukocytes.

5. The invention has simple preparation process and low cost, does not need organic solvent and chemical reducing agent, and is environment-friendly.

Drawings

FIG. 1 is a scanning electron micrograph of the surface of the polypropylene web material of example 1;

FIG. 2 is a scanning electron micrograph of the surface of the polydopamine coated polypropylene mesh material of example 1;

FIG. 3 is a scanning electron microscope image of the surface of the nano-silver/poly-dopamine/polypropylene composite patch material of example 1;

fig. 4 is a scanning electron microscope image of a cross section of the nano silver/poly dopamine/polypropylene composite patch material of example 1.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it should be understood that the specific embodiments described herein are only for explaining the present invention and do not limit the protection scope of the present invention.

The polypropylene mesh used in the following examples was a medical light-weight patch woven from polypropylene filaments, having a size of 3cm × 3cm and a mesh diameter of 3mm, washed with acetone and deionized water, and dried.