阅读说明：本技术 一种从盐角草中提取植物盐的方法 (Method for extracting plant salt from salicornia europaea ) 是由 王胜 李炳文 曹林波 毛庆莲 袁永 于 2019-11-11 设计创作，主要内容包括：本发明公开了一种从盐角草中提取植物盐的方法,包括以下步骤：(1)取盐角草新鲜茎叶上部以质量比1：3～5加入蒸馏水进行研磨,研磨浆液经过滤机过滤后,滤液使用离心机分离,分离出上清液；(2)使用超临界流体CO<Sub>2</Sub>协同夹带剂在20～40MPa、25～50℃下对上清液在萃取釜中进行萃取得到萃取液；(3)萃取液流出萃取釜经减压阀后,CO<Sub>2</Sub>呈气相溢出放空,剩余液相脱色后经真空干燥去除夹带剂得到最终液相；(4)最终液相经低温真空干燥后得到固体植物盐产品。所述夹带剂为乙醇和苯磺酸。本发明使用临界流体CO<Sub>2</Sub>协同夹带剂,可以将盐角草中的植物盐、氨基酸、黄酮等有用物质提取出来,有利于后续保健食品、化妆品、洗剂和生活环境用品等领域市场应用。(The invention discloses a method for extracting plant salt from salicornia europaea, which comprises the following steps: (1) taking the upper parts of fresh stems and leaves of salicornia europaea in a mass ratio of 1: 3-5, adding distilled water for grinding, filtering the grinding slurry by using a filter, separating the filtrate by using a centrifugal machine, and separating out supernatant; (2) using supercritical fluid CO 2 Extracting the supernatant in an extraction kettle at 25-50 ℃ under 20-40 MPa by using a synergistic entrainer to obtain an extraction liquid; (3) after the extract liquid flows out of the extraction kettle and passes through a pressure reducing valve, CO 2 Overflowing in a gas phase and emptying, decoloring the residual liquid phase, and then removing the entrainer by vacuum drying to obtain a final liquid phase; (4) And finally, carrying out low-temperature vacuum drying on the liquid phase to obtain a solid plant salt product. The entrainer is ethanol and benzene sulfonic acid. The invention uses a critical fluid CO 2 The synergistic entrainer can extract useful substances such as plant salt, amino acid, flavone and the like in the salicornia europaea, and is beneficial to the market application in the fields of subsequent health-care food, cosmetics, lotions, living environment products and the like.)

1. A method for extracting plant salt from salicornia europaea is characterized by comprising the following steps:

(1) cleaning the upper parts of fresh stems and leaves of salicornia europaea, and mixing the cleaned fresh stems and leaves of salicornia europaea in a mass ratio of 1: 3-5, adding distilled water for grinding, filtering the grinding slurry by using a filter, separating the filtrate by using a centrifugal machine, and separating out supernatant;

(2) using supercritical fluid CO₂Extracting the supernatant in an extraction kettle at 25-50 ℃ under 20-40 MPa by using a synergistic entrainer to obtain an extraction liquid;

(3) after the extract liquid flows out of the extraction kettle and passes through a pressure reducing valve, CO₂Overflowing in a gas phase and emptying, decoloring the residual liquid phase, and then removing the entrainer by vacuum drying to obtain a final liquid phase;

(4) and finally, carrying out low-temperature vacuum drying on the liquid phase to obtain a solid plant salt product.

2. The method of claim 1, wherein the plant salt is extracted from salicornia europaea by: the entrainer is ethanol and benzene sulfonic acid.

3. The method of claim 2, wherein the plant salt is extracted from salicornia europaea by: the mass ratio of the ethanol to the supernatant is 1-1.5: 10, the mass ratio of the benzenesulfonic acid to the supernatant is 0.3-0.6: 10.

4. a method of extracting plant salt from salicornia europaea as claimed in any one of claims 1 to 3, wherein: the supercritical fluid CO₂The flow rate is 10-15L/min.

5. The method of claim 2, wherein the plant salt is extracted from salicornia europaea by: in the step (3), the temperature for removing the ethanol by vacuum drying is 40-50 ℃, the vacuum degree is 70-80 KPa, the temperature of the benzenesulfonic acid is 75-85 ℃, and the vacuum degree is 70-80 KPa.

6. The method of claim 1, wherein the plant salt is extracted from salicornia europaea by: and (4) carrying out low-temperature vacuum drying on the final liquid phase at-35 to-50 ℃ and under the vacuum degree of 12 to 45 MPa.

7. The method of claim 1, wherein the plant salt is extracted from salicornia europaea by: the decolorizing agent in the step (3) is activated carbon.

8. The method of claim 1, wherein the plant salt is extracted from salicornia europaea by: the speed of the centrifugal machine in the step (1) is 10000-20000 rpm.

Technical Field

The invention relates to the field of vegetation utilization for saline-alkali soil improvement, in particular to a method for extracting plant salt from salicornia europaea.

Background

Salicornia europaea is one of the most salt-tolerant terrestrial higher plant species reported on earth to date. Due to the remarkable salt uptake capacity and accumulation characteristics, salicornia europaea can be used as one of important means of bioengineering measures and is widely used for the comprehensive improvement of saline-alkali soil. The stems and leaves of salicornia europaea contain rich plant salt, flavone, amino acid, vitamin and trace elements, and can be widely applied to the fields of health-care food, skin care products, lotions and environmental products.

Patent CN105029344A discloses a method for extracting plant salt, which uses halophyte as raw material, and adopts ultrasonic extraction method to extract the plant salt, and then vacuum drying at low temperature to obtain the final product, but the extraction components in the plant salt are not comprehensive, and the extraction rate is general.

Patent CN105919024A discloses a plant salt containing extract of Leptospermum scoparium and its preparation method, which comprises drying, pulverizing, vacuum microwave wall breaking, and supercritical fluid CO₂After extraction, ethyl acetate-n-butanol is used for elution, and then the finished product is obtained by vacuum freeze drying, but the method has complex working procedures, the extract of the method is calyx wood protein, nucleic acid and the like, the plant salt extracted from the common seepweed herb adopts a later-stage adding mode, and the extraction of the plant salt adopts an enzymolysis and ultrasonic mode.

Therefore, at present, for extracting plant salt from saline-alkali soil improvement plant salt corniculate, a simple supercritical fluid CO is not used₂The method of (1).

Disclosure of Invention

In order to solve the technical problem, the invention disclosesNovel supercritical fluid CO₂A method for extracting plant salt from Salicornia Herbacea by adding entrainer is provided.

The technical scheme of the invention is as follows: a method for extracting plant salt from Salicornia herbacea comprises the following steps:

(1) cleaning the upper parts of fresh stems and leaves of salicornia europaea, and mixing the cleaned fresh stems and leaves of salicornia europaea in a mass ratio of 1: 3-5, adding distilled water for grinding, filtering the grinding slurry by using a filter, separating the filtrate by using a centrifugal machine, and separating out supernatant; (2) using supercritical fluid CO₂Extracting the supernatant in an extraction kettle at 25-50 ℃ under 20-40 MPa by using a synergistic entrainer to obtain an extraction liquid; (3) after the extract liquid flows out of the extraction kettle and passes through a pressure reducing valve, CO₂Overflowing in a gas phase and emptying, decoloring the residual liquid phase, and then removing the entrainer by vacuum drying to obtain a final liquid phase; (4) and finally, carrying out low-temperature vacuum drying on the liquid phase to obtain a solid plant salt product.

Further, the entrainer is ethanol and benzene sulfonic acid.

Further, the mass ratio of the ethanol to the supernatant is 1-1.5: 10, the mass ratio of the benzenesulfonic acid to the supernatant is 0.3-0.6: 10.

further, the supercritical fluid CO₂The flow rate is 10-15L/min.

Further, in the step (3), the temperature for removing the ethanol by vacuum drying is 40-50 ℃, the vacuum degree is 70-80 KPa, the temperature of the benzenesulfonic acid is 75-85 ℃, and the vacuum degree is 70-80 KPa.

Further, the temperature of the final liquid phase low-temperature vacuum drying in the step (4) is-35 to-50 ℃, and the vacuum degree is 12 to 45 MPa.

Further, the decoloring agent in the step (3) is activated carbon.

Further, the speed of the centrifugal machine in the step (1) is 10000-20000 rpm.

According to the characteristics of salicornia europaea, the upper parts of stems and leaves of fresh salicornia europaea with high salt content are pretreated by grinding, filtering, centrifugal separation and the like, and then are subjected to supercritical fluid CO₂Extracting the filtrate by using a synergistic entrainer, and finally preparing the plant salt with more beneficial components through a separation step, wherein the plant salt has higher yield and better effective performance; at the same timeSimple process and low operation cost. The invention has potential market value.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without inventive labor.

FIG. 1 is a graph showing the antioxidant activity of Salicornia europaea extract plant salt of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.