阅读说明：本技术 一种增产型植物生长调节剂及其应用 (Yield-increasing plant growth regulator and application thereof ) 是由 李志江 林建海 楚娟娟 于 2019-11-28 设计创作，主要内容包括：本发明公开了一种增产型植物生长调节剂及其应用,包括有活性成分和增效安全剂,所述活性成分由如下重量份的组分组成：芸苔素内酯0.001~1份、苄氨基嘌呤0.5~5份和三十烷醇0.05~2份,所述增效安全剂由如下重量份的组分组成：聚谷氨酸1~3份、白芷醇提物1~5份和桑叶醇提物1~5份。本发明将特征组分配方的活性成分和增效安全剂复配,在玉米种植中提高最终收获产量,并在抑制黄瓜霜霉病方面获得了预料不到的技术效果,很大程度地缓解了因杀菌剂使用不当而引起的生长受阻、落花落果、植株死亡等药害。综上所述,本发明能够促进作物的生长,具有增产增收的效果。(The invention discloses a yield-increasing plant growth regulator and application thereof, wherein the yield-increasing plant growth regulator comprises an active ingredient and a synergistic safety agent, and the active ingredient comprises the following components in parts by weight: 0.001-1 part of brassinolide, 0.5-5 parts of benzylaminopurine and 0.05-2 parts of triacontanol, wherein the synergistic safety agent comprises the following components in parts by weight: 1-3 parts of polyglutamic acid, 1-5 parts of angelica dahurica alcohol extract and 1-5 parts of mulberry leaf alcohol extract. According to the invention, the active ingredients of the characteristic component formula and the synergistic safener are compounded, so that the final harvest yield is improved in corn planting, an unexpected technical effect is obtained in the aspect of inhibiting cucumber downy mildew, and the phytotoxicity such as growth retardation, flower and fruit dropping, plant death and the like caused by improper use of the bactericide is relieved to a great extent. In conclusion, the invention can promote the growth of crops and has the effects of increasing both production and income.)

1. The yield-increasing plant growth regulator is characterized by comprising an active ingredient and a synergistic safety agent, wherein the active ingredient comprises the following components in parts by weight: 0.001-1 part of brassinolide, 0.5-5 parts of benzylaminopurine and 0.05-2 parts of triacontanol, wherein the synergistic safety agent comprises the following components in parts by weight: 1-3 parts of polyglutamic acid, 1-5 parts of angelica dahurica alcohol extract and 1-5 parts of mulberry leaf alcohol extract.

2. A yield-increasing plant growth regulator according to claim 1, wherein the active ingredient accounts for 1.5-3.7% by mass of the plant growth regulator, and the synergistic safener accounts for 1.5-10% by mass of the plant growth regulator.

3. A yield-increasing plant growth regulator according to claim 2, wherein the brassinolide is selected from one of 28-homobrassinolide, 24-epibrassinolide, 25-methyl brassinolide, propionyl brassinolide, 28-epibrassinolide, mixotrophin brassinolide and 14-hydroxy brassinosteroid.

4. A method for preparing a plant growth regulator according to any one of claims 1 to 3, wherein the raw materials in the weight ratio are uniformly mixed and prepared into a suspension, a water dispersible granule, a dispersible oil suspension or a solution with a pesticide-allowable auxiliary agent.

5. The method for preparing a yield-increasing plant growth regulator according to claim 4, wherein the method for preparing the radix angelicae alcohol extract comprises the following steps:

cleaning radix angelicae, crushing by a dry method, adding ethanol with the weight being 3-5 times that of the radix angelicae, standing for 20-30 min, adjusting the pH value to 5.6-6.0 by using NaAc-HAc buffer solution, refluxing for 1-3 h at 78-80 ℃, collecting a refluxing extracting solution, carrying out reduced pressure concentration until the relative density is 1.05-1.1 g/cm, carrying out spray drying, and thus obtaining the radix angelicae alcohol extract.

6. The method for preparing a yield-increasing plant growth regulator according to claim 5, wherein a spray dryer is used for spray drying, the air inlet temperature is 130-150 ℃, the air outlet temperature is 100-120 ℃, and the feeding speed is 3-5 ml/min.

7. The method for preparing a yield-increasing plant growth regulator of claim 4, wherein the method for preparing the mulberry leaf alcohol extract comprises the following steps:

cleaning mulberry leaves, crushing the mulberry leaves to 30-70 meshes by a dry method, adding ethanol with the weight being 2-3 times that of the mulberry leaves, carrying out ultrasonic pretreatment for 5-10 min, carrying out water bath leaching for 30-45 min, filtering, leaching twice, combining filtrates, cooling to 50-60 ℃, carrying out rotary evaporation concentration until the relative density is 1.02-1.2 g/cm, carrying out vacuum freeze drying for 24-48 h, and thus obtaining the mulberry leaf alcohol extract.

8. Use of the plant growth regulator according to any one of claims 1 to 3 for promoting the production of strong corn seeds.

9. Use of a plant growth regulator according to any one of claims 1 to 3 for inhibiting cucumber downy mildew.

Technical Field

The invention relates to the technical field of agricultural growth regulators, in particular to a yield-increasing type plant growth regulator and application thereof.

Background

Corn is the first large grain crop in China, and is also an important feed crop, energy crop and industrial raw material. The northeast plain is a main production area of spring corn in China and is also an important commercial grain base in China, and the guarantee of the stable yield and the high yield of the corn has important significance for guaranteeing the grain safety in China.

However, the cold damage at low temperature is a main limiting factor for high and stable yield of regional corns and is also one of important natural disasters for damaging the corn production in high latitude areas and local hills. Due to geographical conditions, severe low temperature occurs in the region every 3-5 years, and the cold and cool mountainous regions in the east are attacked more frequently by low temperature stress in spring. The yield reduction range of spring corn caused by low-temperature cold damage is more than 15% every year, and the yield loss of the spring corn in severe years can reach 20%. The low temperature can reduce the vitality of seeds, delay the time of emergence, seriously cause the shortage of powder seeds or the death of seedlings, and is one of the important reasons for seriously reducing the yield and the quality of the corns. Therefore, it is a problem to be solved in production.

In the domestic and foreign corn production, the loss of adverse climatic factors such as low temperature and the like to agricultural production is reduced and slowed down by applying a mulching film covering technology, breeding cold-resistant varieties of crops and spraying a plant growth regulator. However, the cost of the mulching film covering technology is high, the mulching film can cause white pollution after long-term use, the breeding period of new crop varieties is generally 5-7 years, the breeding period is long, and the application effect is not obvious due to the influence of regional ecological adaptability and other factors. Therefore, the use of plant growth regulators is considered to be a poor alternative to solve adverse abiotic stresses such as low temperature.

However, the varieties of low-temperature resistant plant growth regulators applied in the current production are various, wherein gibberellin, indoleacetic acid, brassinolide and the like are common plant growth regulators, and the plant growth regulators have certain effects on inducing and responding to improve low-temperature stress signals of plants, starting self defense systems and improving low-temperature resistance of crops. However, these natural plant growth regulators are expensive, have single action and no outstanding effect, which greatly limits the range of applications.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provides a yield-increasing plant growth regulator and a preparation method and application thereof.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

the yield-increasing plant growth regulator comprises an active ingredient and a synergistic safety agent, wherein the active ingredient comprises the following components in parts by weight: 0.001-1 part of brassinolide, 0.5-5 parts of benzylaminopurine and 0.05-2 parts of triacontanol, wherein the synergistic safety agent comprises the following components in parts by weight: 1-3 parts of polyglutamic acid, 1-5 parts of angelica dahurica alcohol extract and 1-5 parts of mulberry leaf alcohol extract.

Preferably, the active ingredient accounts for 1.5-3.7% of the plant growth regulator by mass, and the synergistic safety agent accounts for 1.5-10% of the plant growth regulator by mass.

Preferably, the brassinolide is selected from one of 28-homobrassinolide, 24-epibrassinolide, 25-methyl brassinolide, propionyl brassinolide, 28-epibrassinolide, mixotrophin brassinolide or 14-hydroxy brassinosteroid.

The invention also provides a method for preparing the plant growth regulator, which is characterized in that the raw materials in the weight ratio are uniformly mixed and are prepared into a suspending agent, a water dispersible granule, a dispersible oil suspending agent or a solution agent with an auxiliary agent allowed in pesticides.

Preferably, the preparation method of the angelica dahurica alcohol extract comprises the following steps:

cleaning radix angelicae, crushing by a dry method, adding ethanol with the weight being 3-5 times that of the radix angelicae, standing for 20-30 min, adjusting the pH value to 5.6-6.0 by using NaAc-HAc buffer solution, refluxing for 1-3 h at 78-80 ℃, collecting a refluxing extracting solution, carrying out reduced pressure concentration until the relative density is 1.05-1.1 g/cm, carrying out spray drying, and thus obtaining the radix angelicae alcohol extract.

Preferably, the spray dryer is adopted for spray drying, the air inlet temperature is 130-150 ℃, the air outlet temperature is 100-120 ℃, and the feeding speed is 3-5 ml/min.

Preferably, the preparation method of the mulberry leaf alcohol extract comprises the following steps:

cleaning mulberry leaves, crushing the mulberry leaves to 30-70 meshes by a dry method, adding ethanol with the weight being 2-3 times that of the mulberry leaves, carrying out ultrasonic pretreatment for 5-10 min, carrying out water bath leaching for 30-45 min, filtering, leaching twice, combining filtrates, cooling to 50-60 ℃, carrying out rotary evaporation concentration until the relative density is 1.02-1.2 g/cm, carrying out vacuum freeze drying for 24-48 h, and thus obtaining the mulberry leaf alcohol extract.

The invention also aims to protect the application of the plant growth regulator in promoting the strong corn seeds and increasing the yield.

The invention also aims to protect the application of the plant growth regulator in inhibiting cucumber downy mildew.

The functions of the components in the invention are as follows:

brassinolide, a novel green environment-friendly plant growth regulator, is treated by soaking seeds and spraying stems and leaves of brassinolide with proper concentration. Can promote the growth of vegetables, melons, fruits and other crops, improve the quality, increase the yield, and ensure bright color and thicker leaves. The tea leaf picking time can be advanced, and the melon and fruit can have higher sugar content, larger individual, higher yield and better storage stability. Is harmless to human and livestock, and has safe and effective dosage when used normally. The natural brassin can be widely applied to various economic crops, can increase the yield by 5-10% and can reach 30% higher, obviously improve the quality, increase the sugar content and the weight of fruits and increase the gorgeous crops. Meanwhile, the drought resistance and the cold resistance of crops can be improved, and the symptoms of plant diseases and insect pests, phytotoxicity, fertilizer damage and freezing damage of the crops can be relieved.

Benzylaminopurine, which is a synthetic cytokinin plant growth regulator with purine rings, has high cytokinin activity and is mainly used for promoting cell division, enlargement and elongation; inhibit chlorophyll degradation, increase amino acid content, and delay leaf yellowing and aging; inducing the differentiation of tissues (cambium) and organs (buds and roots), promoting the germination of lateral buds and promoting the branching; the fruit setting rate is improved, and seedless fruits are formed; the air holes of the blades are adjusted to be open, the service life of the blades is prolonged, and the fresh-keeping is facilitated. The method is mainly used for regulating growth of Chinese cabbage and citrus and increasing yield.

Triacontanol is a plant growth promoter with a wide application range, has a good yield-increasing effect on rice, cotton, wheat, soybean, corn, sorghum, tobacco, beet, peanut, vegetable, flower, fruit tree, sugarcane and the like, and the yield-increasing amplitude is more than 10%. Is also a high-efficiency and rapid plant growth promoter, and can have remarkable promoting effect on plant growth at very low concentration. When the fertilizer is used as a foliar nutrient fertilizer for crops, the yield of the crops with the planting area of 0.1 hectare can be increased by 10-40% only by 1 mg; the yield-increasing effect of about 20 percent can be obtained by spraying triacontanol with the concentration of 0.5mg/kg in the tea bud one-leaf period.

The polyglutamic acid, also called natto bacteria glue, polyglutamic acid, it is a water-soluble, biodegradable, toxicity-free biological macromolecule made up by using microbial fermentation method, gamma-PGA polyglutamic acid is a viscous substance, found for the first time in fermented bean of "natto", gamma-PGA polyglutamic acid is a special anion natural polymer, it is α -amino (α -amino) and gamma-carboxyl (gamma-carboxyl) through amide bond (amide linkage) gamma-PGA molecular weight of homotypic polyamide (homo-polyamide) gamma-PGA from 5 to 2 million daltons, can increase the yield As plant nutrient, when flooding in the soil, will form a layer of film on the surface layer of plant root hair, not only have the function of protecting root hair, but also have the best transport platform of Cd, moisture and root hair intimate contact in the soil, can effectively improve dissolution, storage, transport and absorption of fertilizer, prevent, precipitate oxalate, root and metal element from producing and absorbing Cd, moisture and root hair, make the soil to increase the yield of plant, and soil to be able to increase the yield of soil, and to make the plant disease-soil, alkali and soil to increase the yield of crops, and soil to be able to increase the soil for a long time, and to increase the soil.

Bai Zhi is warm in nature and pungent in flavor. It enters stomach, large intestine and lung meridians. Dispelling pathogenic wind, removing dampness, inducing resuscitation, relieving pain, relieving swelling, and expelling pus. Contains volatile oil. Twenty-nine compounds with high contents of methyl cyclo-decane, 1-tetradecene, ethyl laurate and several furocoumarins are detected from the volatile oil. In addition, it also contains imperatorin, angelicin, scopoletin, byak-angelicin, isoimperatorin, hydrated byak-angelicin, iso-hui-angelicin, oxypeucedanin, anethole, carvacrol, palmitic acid, etc.

Mulberry leaf, mulberry leaf, cold in nature, sweet and bitter in taste. It enters lung and liver meridians. Disperse wind-heat, clear lung-heat and moisten dryness, clear liver and improve vision. Contains flavonoid, steroid and triterpene, achyranthes bidentata sterone, ecdysterone, rutin, quercetin, chlorogenic acid, coumarin, alkaloid, chlorogenic acid and trace amount of volatile oil.

According to the invention, the active ingredients of the characteristic component formula and the synergistic safener are compounded, an unexpected technical effect is obtained in corn planting, the photosynthetic performance of leaves in the corn grain filling period can be obviously improved, grain filling is promoted, and the final harvest yield is improved. The method has the advantages that the unexpected technical effect is obtained in the aspect of inhibiting the cucumber downy mildew, and the phytotoxicity such as growth obstruction, flower and fruit dropping, plant death and the like caused by improper use of the bactericide is relieved to a great extent. In conclusion, the invention can optimize the quality of crops, promote the growth of the crops and has the effects of increasing the production and income.

Detailed Description

The following is a detailed description of specific embodiments of the invention.