阅读说明：本技术 一种复合肽盐及其制备方法 (Composite peptide salt and preparation method thereof ) 是由 张佛心 陆杰 张朝臣 刘之锋 杨飏 刘一博 王佳佳 于 2019-10-18 设计创作，主要内容包括：本发明属于盐复合调料技术领域,特别是一种复合肽盐及其制备方法,所述的制备方法包括：(1)向多孔淀粉中加入水,搅拌形成多孔淀粉浆液,边搅拌边加入多肽粉,对得到的混合浆液进行超声处理；(2)利用食盐调制得到高浓度食盐水,并将高浓度食盐水以喷雾的方式喷入到混合浆液中,待喷完后,继续搅拌处理3-5分钟；(3)对步骤(2)中制备得到的混合物进行蒸发浓缩,喷雾干燥,得到所述的复合肽盐；本发明利用多孔淀粉作为载体,将多肽粉与食盐复合形成复合肽盐,不仅提高了多肽粉在储存、运输过程中的稳定性,降低了流失率,还提高了其在高温烹饪过程中的稳定性,确保了添加在复合肽盐中的多肽粉被高效的利用。(The invention belongs to the technical field of salt compound seasonings, and particularly relates to a compound peptide salt and a preparation method thereof, wherein the preparation method comprises the following steps: (1) adding water into porous starch, stirring to form porous starch slurry, adding polypeptide powder while stirring, and performing ultrasonic treatment on the obtained mixed slurry; (2) preparing salt to obtain high-concentration salt water, spraying the high-concentration salt water into the mixed slurry in a spraying manner, and continuously stirring for 3-5 minutes after spraying; (3) evaporating and concentrating the mixture prepared in the step (2), and performing spray drying to obtain the compound peptide salt; according to the invention, the porous starch is used as a carrier, and the polypeptide powder and the salt are compounded to form the composite peptide salt, so that the stability of the polypeptide powder in the processes of storage and transportation is improved, the loss rate is reduced, the stability of the polypeptide powder in the process of high-temperature cooking is improved, and the efficient utilization of the polypeptide powder added in the composite peptide salt is ensured.)

1. The preparation method of the compound peptide salt is characterized by comprising the following steps:

(1) adding water with the weight 2-3 times that of the porous starch into the porous starch, stirring to form porous starch slurry, then adding polypeptide powder while stirring, and after the polypeptide powder is added, carrying out ultrasonic treatment on the obtained mixed slurry;

(2) preparing salt to obtain high-concentration salt solution, then stirring the mixed slurry in the step (1) at a high speed, spraying the high-concentration salt solution into the mixed slurry in a spraying manner, and continuing stirring for 3-5 minutes after spraying;

(3) and (3) evaporating and concentrating the mixture prepared in the step (2), and performing spray drying to obtain the compound peptide salt.

2. The method for preparing the complex peptide salt according to claim 1, wherein the method for preparing the porous starch comprises: adding acetic acid-sodium acetate buffer solution with the pH value of 4-5 into corn starch, adding compound enzyme solution with the mass of 0.5-1.0% of that of the corn starch, carrying out enzymolysis treatment at the temperature of 40-45 ℃ for 8-10h, adding alkaline solution into the corn starch subjected to enzymolysis for neutralization, then carrying out suction filtration and water washing, adding water into the obtained corn starch to form slurry, adjusting the pH value of the slurry to 9-11, then adding oxalic acid under the stirring condition, carrying out stirring reaction for 1-2h, then adding alkaline solution for neutralization, carrying out suction filtration again, carrying out water washing, and then placing the obtained starch in a vacuum drying oven for drying to obtain the porous starch.

3. The method for preparing the complex peptide salt according to claim 2, wherein the complex enzyme solution is formed by compounding α -amylase and glucoamylase, and the mass ratio of the α -amylase to the glucoamylase is 1 (1-3).

4. The method for preparing a complex peptide salt according to claim 1, wherein the polypeptide powder is one or more selected from the group consisting of collagen peptide powder, soybean peptide powder, corn oligopeptide powder, wheat oligopeptide powder, and casein phosphopeptide powder.

5. The method for preparing a complex peptide salt according to claim 1, wherein the ultrasonic treatment conditions in step (1) include: the power is 600-1200w, and the treatment time is 30-45 minutes.

6. The method for producing a complex peptide salt according to claim 1, wherein the mass concentration of the high-concentration saline solution is 35 to 50%.

7. The method of preparing a complex peptide salt according to claim 1, wherein the spray-drying conditions comprise: the air inlet temperature is 160-170 ℃, the temperature is 2 ℃, the air outlet temperature is 80-90 ℃ and the temperature difference is 1 ℃.

8. A complex peptide salt prepared by the preparation method of any one of claims 1 to 7.

Technical Field

The invention belongs to the technical field of salt compound seasonings, and particularly relates to a compound peptide salt and a preparation method thereof.

Background

The amino group of one amino acid and the carboxyl group of another amino acid can be condensed into a peptide, the formed amide group is called peptide bond in protein chemistry, the molecule of the amino acid is the smallest, the protein is the largest, two or more amino acids are dehydrated and condensed to form a plurality of peptide bonds to form a peptide, and a plurality of peptides are folded in multiple stages to form a protein molecule. Scientific research in recent years has suggested that the main form of protein absorption by the human body is not in the form of amino acids, but in the form of polypeptides. The peptide has strong activity and diversity, can comprehensively regulate the physiological function of a human body and enhance the physiological activity of the human body, so the peptide is taken as a part of daily intake of the human body and has important significance for improving the function of the human body.

Salt is the most basic seasoning in daily diet, therefore, the prior art has a scheme of compounding peptides into salt to form peptide salt, and the peptide salt provides required nutrition for human bodies. Specifically, for example, chinese patent with application number "201510486226.1" and invention name "a corn peptide salt" discloses a technical scheme for mixing and stirring corn peptide powder and salt at room temperature to obtain the corn peptide salt. However, this simple mixing approach does not facilitate storage of the peptide powder.

Disclosure of Invention

The invention aims to overcome the defects in the prior art, and provides a preparation method of a composite peptide salt, which realizes the uniform mixing of polypeptide and salt and can ensure the stability of peptide powder in the processes of salt storage and high-temperature cooking.

In order to achieve the purpose, the invention adopts the following technical scheme:

a preparation method of a composite peptide salt comprises the following steps:

(1) adding water with the weight 2-3 times that of the porous starch into the porous starch, stirring to form porous starch slurry, then adding polypeptide powder while stirring, and after the polypeptide powder is added, carrying out ultrasonic treatment on the obtained mixed slurry;

(2) preparing salt to obtain high-concentration salt solution, then stirring the mixed slurry in the step (1) at a high speed, spraying the high-concentration salt solution into the mixed slurry in a spraying manner, and continuing stirring for 3-5 minutes after spraying;

(3) and (3) evaporating and concentrating the mixture prepared in the step (2), and performing spray drying to obtain the compound peptide salt.

The porous starch is a granular substance with porous adsorption property, has porosity and large specific surface area, can adsorb liquid or solid substances in a dry substance state and is directly prepared to form a powdery mixture; according to the technical scheme provided by the invention, porous starch is used as a carrier, water is added into the porous starch to form slurry, polypeptide powder is added into the slurry, polypeptide is invaded into pores of the porous starch in an ultrasonic oscillation mode, high-concentration salt water is sprayed into the mixed slurry in a spraying mode to obtain salt-coated porous starch, and the composite peptide salt is obtained through evaporation concentration and spray drying.

According to the composite peptide salt provided by the invention, the porous starch is used as a carrier of the polypeptide powder, and then the high-concentration salt solution is sprayed on the surface of the porous starch to form a coating structure, so that the stability of the salt seasoning compounded with the polypeptide powder in the processes of storage, transportation and high-temperature cooking is improved; in addition, the salt seasoning added with the polypeptide powder is taken by a human body every day, so that the polypeptide powder can fully play the functions of regulating the physiological function of the human body and enhancing the physiological activity of the human body.

Further, in the invention, the preparation method of the porous starch comprises the following steps: adding acetic acid-sodium acetate buffer solution with the pH value of 4-5 into corn starch, adding compound enzyme solution with the mass of 0.5-1.0% of that of the corn starch, carrying out enzymolysis treatment at the temperature of 40-45 ℃ for 8-10h, adding alkaline solution into the corn starch subjected to enzymolysis for neutralization, then carrying out suction filtration and water washing, adding water into the obtained corn starch to form slurry, adjusting the pH value of the slurry to 9-11, then adding oxalic acid under the stirring condition, carrying out stirring reaction for 1-2h, then adding alkaline solution for neutralization, carrying out suction filtration again, carrying out water washing, and then placing the obtained starch in a vacuum drying oven for drying to obtain the porous starch. The composite enzyme solution acts on the starch raw material to synthesize the porous starch, and then adipic acid is used as a cross-linking agent to react with the porous starch, so that the structural strength of the porous starch is improved, and the stability of the porous starch is ensured.

Furthermore, in the invention, the compound enzyme solution is formed by compounding α -amylase and glucoamylase, and the mass ratio of the α -amylase to the glucoamylase is 1 (1-3).

In the invention, the polypeptide powder is also called albumin polypeptide powder, is an important small molecular substance, is used as basic functional protein, can be directly used for supplementing human protein, and has important functions of maintaining plasma colloid osmotic pressure, assisting cell metabolism and the like. Specifically, the polypeptide powder is one or more of ossein peptide powder, soybean peptide powder, corn oligopeptide powder, wheat oligopeptide powder and casein phosphopeptide powder.

In the present invention, the fusion of the polypeptide powder and the porous starch is promoted by means of ultrasonic treatment, and as a preferred condition, in the step (1), the ultrasonic treatment condition includes: the power is 600-1200w, and the treatment time is 30-45 minutes.

In the present invention, the inventors of the present invention have found that a coating type structure is not easily formed if the concentration of the saline solution is too low or too high by spraying the saline solution onto the porous starch having adsorbed the polypeptide powder, and preferably, the mass concentration of the high-concentration saline solution is 35 to 50%.

In the present invention, the powder is obtained by spray drying, and preferably, the spray drying conditions include: the air inlet temperature is 160-170 ℃, the temperature is 2 ℃, the air outlet temperature is 80-90 ℃ and the temperature difference is 1 ℃.

The compound peptide salt prepared by the method has the advantages of rich nutrition and convenient use, is used as a common seasoning in daily life, and has important significance for promoting human health.

Compared with the prior art, the invention has the following technical effects:

according to the preparation method of the composite peptide salt, the porous starch is used as a carrier, and the polypeptide powder and the salt are compounded to form the composite peptide salt, so that the stability of the polypeptide powder in the processes of storage and transportation is improved, the loss rate is reduced, the stability of the polypeptide powder in the process of high-temperature cooking is improved, and the polypeptide powder added in the composite peptide salt is efficiently utilized. In addition, the preparation method of the compound peptide salt provided by the invention is simple and convenient, and is easy for mass production.

Additional features and advantages of the invention will be set forth in the detailed description which follows.

Detailed Description

In order to make the technical means, the creation features, the achievement purposes and the effects of the invention easy to understand, the invention is further clarified with the specific embodiments.

The invention provides a preparation method of a composite peptide salt, which comprises the following specific steps:

(1) adding water with the weight 2-3 times that of the porous starch into the porous starch, stirring to form porous starch slurry, then adding polypeptide powder while stirring, and after the polypeptide powder is added, carrying out ultrasonic treatment on the obtained mixed slurry;

wherein, the addition amount of the polypeptide powder is preferably 3-5% of the weight of the porous starch, and the excessive polypeptide powder is not easy to be completely adsorbed and is easy to cause waste;

(2) preparing salt to obtain high-concentration salt solution, then stirring the mixed slurry in the step (1) at a high speed, spraying the high-concentration salt solution into the mixed slurry in a spraying manner, and continuing stirring for 3-5 minutes after spraying;

(3) and (3) evaporating and concentrating the mixture prepared in the step (2), and performing spray drying to obtain the compound peptide salt.

The preparation method of the complex peptide salt provided by the present invention is further illustrated by the following specific examples.

The invention provides the following examples:

the corn starch is purchased from Shandong Rui Hongze chemical engineering Co., Ltd, the acetic acid-sodium acetate buffer solution is purchased from Cheng Onreisi chemical reagent Co., Ltd, the product number is R172593, the α -amylase is purchased from Zheng Zhou Qihuadun chemical product Co., Ltd, the glucoamylase is purchased from Wuhanfeng Zhulin chemical engineering Co., Ltd, and the soybean peptide powder is purchased from Hubei such as Tiansheng engineering Co., Ltd.

The remaining raw materials, the sources of which are not particularly limited, may be purchased commercially or prepared according to conventional methods well known to those skilled in the art; the purity is not particularly limited, and the present invention preferably employs a purity which is conventionally used in the field of analytical purification or composite materials.