阅读说明：本技术 以玉米淀粉工艺水制备玉米蛋白肽的方法 (Method for preparing corn protein peptide by using corn starch process water ) 是由 赵玉斌 王成田 王灵云 孔磊 于 2019-11-27 设计创作，主要内容包括：本发明公开了一种以玉米淀粉工艺水制备玉米蛋白肽的方法,属于玉米加工技术领域。解决了玉米淀粉工艺水的处理问题,所述的以玉米淀粉工艺水制备玉米蛋白肽的方法,包括以下步骤：以固形物含量为3-5％的玉米淀粉工艺水为原料,在pH值为2-4,温度为40-55℃条件下向玉米工艺水中加入蛋白酶,酶解6-10h后精制过滤,所得滤液经喷雾干燥后得玉米蛋白肽。本发明可用于处理玉米淀粉工艺水。(The invention discloses a method for preparing corn protein peptide by using corn starch process water, belonging to the technical field of corn processing. The method for preparing the corn protein peptide by using the corn starch process water solves the problem of treatment of the corn starch process water, and comprises the following steps: taking corn starch process water with solid content of 3-5% as raw material, adding protease into the corn process water under the conditions of pH value of 2-4 and temperature of 40-55 ℃, performing enzymolysis for 6-10h, refining and filtering, and performing spray drying on the obtained filtrate to obtain the corn protein peptide. The invention can be used for treating corn starch process water.)

1. A method for preparing corn protein peptide by using corn starch process water is characterized by comprising the following steps: taking corn starch process water with solid content of 3-5% as raw material, adding protease into the corn process water under the conditions of pH value of 2-4 and temperature of 40-55 ℃, performing enzymolysis for 6-10h, refining and filtering, and performing spray drying on the obtained filtrate to obtain the corn protein peptide.

2. The method of claim 1 for preparing zein peptide with corn starch process water, characterized in that: the protease is one or two of acid protease or flavourzyme.

3. The method of claim 2 for preparing zein peptide with corn starch process water, characterized in that: the addition amount of the acid protease is 0.3-1.0% of the corn starch process water-drying base.

4. The method of claim 2 for preparing zein peptide with corn starch process water, characterized in that: the addition amount of the flavourzyme is 0.05-0.5% of the corn starch process water-drying base.

5. The method of claim 1 for preparing zein peptide with corn starch process water, characterized in that: the refining and filtering process is a three-stage membrane treatment technology, and the three-stage membrane is a microfiltration membrane with the diameter of 3 mu m, an ultrafiltration membrane with the diameter of 5nm and a nanofiltration membrane with the diameter of 1nm in sequence.

6. The method of claim 5 for preparing zein peptide with corn starch process water, characterized in that: the working pressure of the microfiltration membrane, the ultrafiltration membrane and the nanofiltration membrane is 0.1MPa, 1MPa and 1MPa in sequence.

7. The method of claim 1 for preparing zein peptide with corn starch process water, characterized in that: the air inlet temperature of the spray drying is 110-120 ℃.

8. The method of any one of claims 1-7 for preparing zein peptide with corn starch process water, characterized in that: the zein peptide has a purity of greater than 95%.

9. The method of any one of claims 1-7 for preparing zein peptide with corn starch process water, characterized in that: the extraction rate of the corn protein peptide is more than 70%.

Technical Field

The invention relates to a method for preparing corn protein peptide by using corn starch process water, belonging to the technical field of corn processing.

Background

The waste water produced in the wet production of starch from corn is called corn starch process water. At present, three methods for treating corn starch process water mainly exist in enterprises, one is that solid content in the corn starch process water is low, so most of the enterprises directly discharge the corn starch process water, but the corn starch process water contains a large amount of organic matters such as protein and the like, is rich in nutrient substances and directly discharges the corn starch process water, so that environmental pollution is caused, the corn starch process water is difficult to treat, and meanwhile, materials are wasted; the other mode is recycling, but because the corn starch process water contains a large amount of soluble protein, the soluble protein is repeatedly brought into the working procedure in the recycling process, particularly the acid making working procedure, the high temperature causes the denaturation of the protein and other substances, and the scaling is caused in the equipment pipeline; there is also a way to add a flocculant to the corn starch process water to flocculate the protein, but the flocculation is far from optimal. Therefore, a method for treating corn starch process water is needed, wherein the method can not only excavate useful components of the corn starch process water, improve application value, but also solve environmental pollution.

Disclosure of Invention

The technical problem to be solved by the invention is as follows: overcomes the defects of the prior art and provides a method for preparing corn protein peptide by using corn starch process water.

The method for preparing the corn protein peptide by using the corn starch process water comprises the following steps: taking corn starch process water with solid content of 3-5% as raw material, adding protease into the corn process water under the conditions of pH value of 2-4 and temperature of 40-55 ℃, performing enzymolysis for 6-10h, refining and filtering, and performing spray drying on the obtained filtrate to obtain the corn protein peptide.

When the starch is produced by a wet method, the corn needs to be soaked by sulfurous acid, so the produced corn starch process water is acidic, and can be directly used as a raw material to extract corn protein peptide under most conditions.

Preferably, the protease is one or two of acid protease or flavor protease.

Preferably, the addition amount of the acid protease is 0.3-1.0% of the dry basis of the corn starch process water.

Preferably, the addition amount of the flavourzyme is 0.05-0.5% of the corn starch process water-dry base.

Preferably, the refining filtration process is a three-stage membrane treatment technology, and the three-stage membrane is a microfiltration membrane with the diameter of 3 microns, an ultrafiltration membrane with the diameter of 5nm and a nanofiltration membrane with the diameter of 1nm in sequence. The microfiltration membrane can filter macromolecular protein, the ultrafiltration membrane can filter macromolecular material salinity, the nanofiltration membrane can filter micromolecular impurity and salinity to improve zein peptide's purity.

The three-stage membrane treatment technology also has the function of concentrating materials and is used for preparing for later-stage spray drying.

Preferably, the working pressure of the microfiltration membrane, the ultrafiltration membrane and the nanofiltration membrane is 0.1MPa, 1MPa and 1MPa in sequence.

Preferably, the inlet air temperature of the spray drying is 110-120 ℃.

The purity of the corn protein peptide prepared by the method is more than 95 percent, and the extraction rate of the corn protein peptide is more than 70 percent. The extraction rate refers to that the product after protease enzymolysis is macromolecular protein and crude product zein peptide, and the ratio of the finally obtained zein peptide with the purity of more than 95 percent to the crude product zein peptide is more than 70 percent.

Compared with the prior art, the invention has the beneficial effects that:

the invention takes corn starch process water as raw material, and produces high-purity corn protein peptide by enzymolysis and refining treatment according to the characteristics of the composition of the process water and the acidity of the material, thereby not only solving the problem of processing by-products and protecting the environment, but also recycling the material and improving the added value of the material, and having important significance for the healthy development of the starch sugar industry in China.

Detailed Description

The present invention will be further described with reference to the following specific examples.