阅读说明：本技术 一种碎米中蛋白质的高效提取率的方法 (Method for high-efficiency extraction of protein in broken rice ) 是由 甘启斌 于 2019-12-03 设计创作，主要内容包括：本发明公开一种碎米中蛋白质的高效提取率的方法,包括以下步骤：碎米去杂和粉碎,微碎米浸泡,静置离心,加入丙酮混合液,静置得到碎米混合液；加入氢氧化钠浸泡,调节pH值,离心,弃去上清液,得到粗蛋白,粗蛋白经蒸馏水清洗,干燥冷却,保存,获得粗蛋白静置液,对粗蛋白静置液进行酸碱度调节,调节PH至8.7-9.2,并加入蛋白酶,获得料液；用离心机进行两级离心分离,以使得料液分离,保留离心液,喷雾干燥,得蛋白粉。本发明通过对碎米进行粉碎、浸泡、制备粗蛋白等处理过程,可使碎米中的蛋白质更充分的提取,且减少提取的蛋白质中杂质含量,节约了提取的成本,减少操作步骤,最大化地提高了碎米中的蛋白质的提取率,大大节约了提取的时间。(The invention discloses a method for high-efficiency extraction of protein in broken rice, which comprises the following steps: removing impurities from broken rice, pulverizing, soaking slightly broken rice, standing, centrifuging, adding acetone mixed solution, and standing to obtain broken rice mixed solution; adding sodium hydroxide for soaking, adjusting pH value, centrifuging, removing supernatant to obtain crude protein, washing the crude protein with distilled water, drying, cooling, storing to obtain crude protein standing solution, adjusting pH value of the crude protein standing solution to 8.7-9.2, and adding protease to obtain feed liquid; and (3) performing two-stage centrifugal separation by using a centrifugal machine to separate feed liquid, reserving centrifugal liquid, and performing spray drying to obtain the protein powder. According to the invention, the broken rice is subjected to the treatment processes of crushing, soaking, crude protein preparation and the like, so that the protein in the broken rice can be more fully extracted, the impurity content in the extracted protein is reduced, the extraction cost is saved, the operation steps are reduced, the extraction rate of the protein in the broken rice is maximally improved, and the extraction time is greatly saved.)

1. A method for high-efficiency extraction of protein from broken rice is characterized by comprising the following steps: the method comprises the following steps:

s1, removing impurities from broken rice and crushing: separating bran shells and large-particle broken stones from broken rice by using a grading stone remover, carrying out superfine grinding on the broken rice by using a superfine grinder, and collecting filtered superfine rice;

s2, soaking the micro-crushed rice: soaking the micro-crushed rice in water, and stirring by a rotating speed machine;

s3, standing: standing and centrifuging the stirred mixed solution, retaining a precipitate, adding an acetone mixed solution into the precipitate, and standing to obtain a broken rice mixed solution;

s4, preparation of crude protein: adding sodium hydroxide into the broken rice mixed solution for soaking, extracting supernatant, adjusting the pH value with 0.2-1mol/L hydrochloric acid solution, centrifuging, and removing supernatant to obtain crude protein;

s5, cooling and storing: washing the crude protein with distilled water, drying, cooling, and storing to obtain crude protein standing solution;

s6, adjusting the pH value of the cooled and stored crude protein standing solution, adjusting the pH value to 8.7-9.2, and adding protease into the standing crude protein solution to obtain a feed liquid;

s7, performing two-stage centrifugal separation on the feed liquid obtained in the step S6 by using a centrifugal machine, wherein the heavy phase is starch slurry, and the light phase is concentrated protein slurry, so that the feed liquid is separated, and the centrifugal liquid is reserved;

s8, spray drying the light phase protein slurry to obtain protein powder.

2. The method for high efficiency extraction of protein from broken rice as claimed in claim 1, wherein: the stirring time in the step S2 is 1-3h, and the stirring speed of the speed rotating machine is 200-600 r/min.

3. The method as claimed in claim 1, wherein in the step S3, the volume ratio of the precipitate to the acetone mixed solution is 100-200:1, and the ketone mixed solution is a mixture of β -mercaptoethanol with a mass fraction of 0.07% and phenylmethylsulfonyl fluoride with a molar concentration of 1mmol/L in a volume ratio of 1: 2-5.

4. The method for high efficiency extraction of protein from broken rice as claimed in claim 1, wherein: and in the step S4, adding 0.05mmol/L sodium hydroxide solution into the mixed liquid of the broken rice according to the solution mass ratio of 2-7: 1.

5. The method of claim 4, wherein the extraction of protein from broken rice is performed with high efficiency: in the step S4, the pH value of the supernatant is adjusted to 4.2-4.5 by using 0.2-1mol/L hydrochloric acid solution.

6. The method of claim 4, wherein the extraction of protein from broken rice is performed with high efficiency: in the step S6, the mass ratio of the protease to the crude protein standing liquid is 4-9:1000, the reaction temperature is 30-45 ℃, and the reaction time is 8-16 h.

7. The method of claim 4, wherein the extraction of protein from broken rice is performed with high efficiency: the rotation speed of the centrifuge in the step S7 is 1000-2000 r/min.

Technical Field

The invention belongs to a broken rice protein extraction method, and relates to a method for high-efficiency extraction of protein in broken rice.

Background

Rice is the most important food crop in the world and is also an important protein source in human food consumption. The rice protein consists of albumin, globulin, prolamine and glutelin, and 95 percent of protein in the rice endosperm exists in the form of proteosome, mainly large spherical proteosome. The rice produces a large amount of broken rice by-products in the processing production process. In recent years, in order to save cost, improve economic benefit and increase the raw material sources of feed proteins, the application degree of broken rice resources in feed formulas is continuously enhanced, but the broken rice proteins are not fully utilized.

At present, the method for extracting protein from broken rice by adopting the prior art has the problems of low protein extraction rate, poor quality and high extraction cost, and in order to solve the problems, a method for efficiently extracting protein from broken rice is designed.

Disclosure of Invention

The invention aims to provide a method for high-efficiency extraction of protein from broken rice, which solves the problems of low extraction rate of protein from broken rice and high extraction cost in the prior art.

The purpose of the invention can be realized by the following technical scheme:

a method for high-efficiency extraction of protein from broken rice comprises the following steps:

s1, removing impurities from broken rice and crushing: separating bran shells and large-particle broken stones from broken rice by using a grading stone remover, carrying out superfine grinding on the broken rice by using a superfine grinder, and collecting filtered superfine rice;

s2, soaking the micro-crushed rice: soaking the micro-crushed rice in water, and stirring by a rotating speed machine;

s3, standing: standing and centrifuging the stirred mixed solution, retaining a precipitate, adding an acetone mixed solution into the precipitate, and standing to obtain a broken rice mixed solution;

s4, preparation of crude protein: adding sodium hydroxide into the broken rice mixed solution for soaking, extracting supernatant, adjusting the pH value with 0.2-1mol/L hydrochloric acid solution, centrifuging, and removing supernatant to obtain crude protein;

s5, cooling and storing: washing the crude protein with distilled water, drying, cooling, and storing to obtain crude protein standing solution;

s6, adjusting the pH value of the cooled and stored crude protein standing solution, adjusting the pH value to 8.7-9.2, and adding protease into the standing crude protein solution to obtain a feed liquid;

s7, performing two-stage centrifugal separation on the feed liquid obtained in the step S6 by using a centrifugal machine, wherein the heavy phase is starch slurry, and the light phase is concentrated protein slurry, so that the feed liquid is separated, and the centrifugal liquid is reserved;

s8, spray drying the light phase protein slurry to obtain protein powder to improve the extraction rate of broken rice protein.

Further, the stirring time in the step S2 is 1-3h, and the stirring speed of the rotary speed machine is 200-600 r/min.

Further, in the step S3, the volume ratio of the precipitate to the acetone mixed solution is 100-200:1, and the ketone mixed solution is a solution prepared by mixing β -mercaptoethanol with the mass fraction of 0.07% and phenylmethylsulfonyl fluoride with the molar concentration of 1mmol/L according to the volume ratio of 1: 2-5.

Further, 0.05mmol/L sodium hydroxide solution is added into the broken rice mixed solution in the step S4 according to the solution mass ratio of 2-7: 1.

Further, in the step S4, the pH value of the supernatant is adjusted to 4.2-4.5 by using 0.2-1mol/L hydrochloric acid solution.

Further, in the step S6, the mass ratio of the protease to the crude protein standing liquid is 4-9:1000, the reaction temperature is 30-45 ℃, and the reaction time is 8-16 h.

Further, the rotation speed of the centrifuge in the step S7 is 1000-.

The invention has the beneficial effects that:

according to the method for efficiently extracting the protein from the broken rice, provided by the invention, the broken rice is subjected to the treatment processes of crushing, soaking, crude protein preparation and the like, so that the protein in the broken rice can be more sufficiently extracted, the impurity content in the extracted protein is reduced, the extraction cost is saved, the operation steps are reduced, the extraction rate of the protein in the broken rice is maximally improved, and the extraction time is greatly saved.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.