Trapping and killing bag matrix for soil-inhabiting termites and preparation method thereof
阅读说明：本技术 土栖白蚁的诱杀包基质及其制备方法 (Trapping and killing bag matrix for soil-inhabiting termites and preparation method thereof ) 是由 潘程远 潘伟华 王方 于 2019-10-29 设计创作，主要内容包括：本发明公开了一种土栖白蚁的诱杀包基质的制备方法,包括以下步骤：试剂溶液由浓度为(0.02±0.002)ng/mL的反-3-己烯-1-醇、0.05～0.1g/mL的葡萄糖、以及作为余量的水组成；甘蔗渣干燥后粉碎；将颗粒装甘蔗渣和试剂溶液按照1g/(1.5±0.1)mL的料液比均匀搅拌混合后,置于黑暗条件下风干得诱杀包基质。本发明还同时提供了上述栖白蚁的诱杀包基质的包装法,将诱杀包基质与化学药剂混合后,加入包装袋内。白蚁对经本发明处理的诱杀包基质的取食量要比未经处理的对照基质的取食量高2-4倍。(The invention discloses a preparation method of a trapping and killing bag matrix for soil-dwelling termites, which comprises the following steps: the reagent solution consists of trans-3-hexene-1-alcohol with the concentration of (0.02 +/-0.002) ng/mL, glucose with the concentration of 0.05-0.1g/mL and water as the rest; drying bagasse and then crushing; uniformly stirring and mixing the granular bagasse filled in the particles and the reagent solution according to the feed-liquid ratio of 1g/(1.5 +/-0.1) mL, and then placing the mixture in the dark for air drying to obtain the trapping and killing bag matrix. The invention also provides a packaging method of the trapping and killing bag matrix for the termites, wherein the trapping and killing bag matrix is mixed with chemical agents and then added into a packaging bag. The feeding amount of the termite to the trapping and killing bag matrix treated by the invention is 2-4 times higher than that of the untreated control matrix.)
1. The preparation method of the trapping and killing bag matrix for the soil-inhabiting termites is characterized by comprising the following steps:
1) preparing a reagent solution:
the reagent solution consists of trans-3-hexene-1-alcohol with the concentration of (0.02 +/-0.002) ng/mL, glucose with the concentration of 0.05-0.1g/mL and water as the rest;
2) drying the bagasse until the water content is (12 +/-2)%, and then crushing to obtain granular bagasse;
3) uniformly stirring and mixing the granular bagasse and the reagent solution according to the feed-liquid ratio of 1g/(1.5 +/-0.1) mL, and then placing the mixture in the dark for air drying to obtain the trapping and killing bag matrix.
2. The method of preparing a termitomyces terrestris trapping matrix according to claim 1, wherein the termitomyces terrestris comprises: and 3) air-drying until the water content of the trapping and killing bag matrix is 15-20%.
3. The method for preparing a termite trapping and killing packet substrate for terrestrial termites as claimed in claim 1 or 2, wherein: the grain size of the bagasse packed in the particles is 1-2 mm.
4. The method for packaging the substrate of the luring and killing bag for the soil-dwelling termites as claimed in any one of claims 1 to 3, which comprises the steps of mixing the substrate of the luring and killing bag with a chemical agent, and then adding the mixture into a packaging bag; the method is characterized in that: a 6 x 10cm self-sealing bag is used as a packaging bag; the weight of the trapping bag substrate packaged in the self-sealing bag is (5 +/-0.5) g.
Technical Field
The invention belongs to the field of termite control, and particularly relates to a trapping and killing bag matrix with an attraction effect on feeding of soil-inhabiting termites and a preparation method thereof.
Background
Soil termites refer to the class of termites that nest termites in the soil, broadly to the genus termites, and the genus termites. In China, common soil-dwelling termites include black-wing white termites, Hainan white-wing termites, yellow-wing white-ridge termites and the like. The termites can generate an all-in-one termite channel underground, and potential risks are generated to dams of reservoirs, ponds, rivers and the like. They damage economic trees (such as Chinese torreya, hickory nut, waxberry and the like), crops (such as sugarcane and corn), garden trees and the like by eating barks, root hairs and the like. Therefore, the soil-dwelling termites are a kind of pests that can cause harm to many aspects of the human society.
The method for preventing and controlling the soil-dwelling termites is various, such as a nest digging method, a pesticide spraying method, a trapping and killing method and the like, wherein the trapping and killing method becomes a main means for preventing and controlling the soil-dwelling termites at present due to the advantages of simple and convenient operation, low dosage, good prevention and control effect and the like. The most common method of trapping is to embed a trapping bag. According to the feeding habits of soil-dwelling termites, the trapping package is usually buried in two time periods of 4-6 months and 8-10 months every year, the termites feed the matrix in the trapping package, the medicines in the matrix are brought back into nests, and then feeding of food among individual termites (feeding behavior) is utilized, so that the whole termite colony is poisoned and finally dies. Therefore, trapping sufficient termites is a prerequisite for successfully utilizing the trapping and killing bag to prevent and control the termites, and the matrix plays a key role.
The trapping and killing bag is generally composed of three parts, namely a substrate, a chemical agent, a paper packaging material and the like. At present, people have less research on the substrate in the trapping and killing bag, and all the research is to use fragments or powder made of wood, bark, forage and the like as the substrate material. Although soil-inhabiting termites can selectively eat wood, bark or forage under natural conditions, the physical and chemical properties of the soil-inhabiting termites can be changed in the process of crushing, processing and packaging the materials to become a trapping package substrate, so that the trapping efficiency of the soil-inhabiting termites is reduced, and the termites cannot be trapped sufficiently.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a trapping and killing bag matrix for soil-inhabiting termites, which has attraction effect on the feeding of the soil-inhabiting termites, and a preparation method thereof.
In order to solve the technical problems, the invention provides a preparation method of a trapping and killing bag matrix for soil-dwelling termites, which comprises the following steps:
1) preparing a reagent solution:
the reagent solution consists of trans-3-hexene-1-alcohol with the concentration of (0.02 +/-0.002) ng/mL, glucose with the concentration of 0.05-0.1g/mL and water as the rest;
2) drying the bagasse until the water content is (12 +/-2)%, and then crushing to obtain granular bagasse;
3) uniformly stirring and mixing the granular bagasse and the reagent solution according to the feed-liquid ratio of 1g/(1.5 +/-0.1) mL, and then placing the mixture in the dark for air drying (indoor natural air drying) to obtain the trapping and killing bag matrix.
The improvement of the preparation method of the trapping and killing bag matrix of the soil-inhabiting termite is as follows: and 3) air-drying until the water content of the trapping and killing bag matrix is 15-20%.
The percentages in the above water contents are all by weight.
The preparation method of the trapping and killing bag matrix for the soil-inhabiting termites is further improved as follows: the grain size of the bagasse packed in the particles is 1-2 mm.
The invention also provides a packaging method of the trapping and killing bag matrix for the soil-dwelling termites, which comprises the steps of mixing the trapping and killing bag matrix with chemical agents (mixing according to the conventional dosage ratio), and then adding the mixture into a packaging bag to obtain the trapping and killing bag. A 6 x 10cm self-sealing bag (made of filter paper) is used as a packaging bag; the weight of the trapping bag substrate packaged in the self-sealing bag is (5 +/-0.5) g.
The valve bag is formed by overlapping two pieces of filter paper of 6 x 10cm and sewing along the edge.
Examples of commonly used chemicals are: fipronil, imidacloprid, fipronil, hexaflumuron, etc.
The attractant for the trapping and killing bag matrix is suitable for soil-inhabiting termites, mainly termites of the genus Termite and the genus Macrotermites.
The reason for air drying in the dark in step 3) of the present invention is to avoid too rapid volatilization and chemical changes of trans-3-hexen-1-ol.
The invention has the beneficial effects that:
1. the feeding amount of the termite to the trapping and killing bag matrix treated by the technical method is 2-4 times higher than that of the untreated control matrix.
2. The invention discovers that: the tightness of the package can also influence the feeding of the termites, so that the matrix prepared by the method is packaged into a bag with certain tightness, so that the attraction amount and the feeding amount of the matrix to the soil-dwelling termites are obviously improved. .
The invention has been carried out the following experiments in the course of the invention:
The matrix material selected in the experiment is wood or fern which is favored by odontotermes formosanus in the field, and is pine, fir, bagasse and pennisetum alopecuroides. The four materials are naturally dried until the water content is basically consistent (about 12%), and are crushed and sieved after being dried, and particles with the diameter of 2mm are retained for the selective feeding experiment of the termites.
The adult winged termites are paired, and the nest colony of the black-wing soil termites which is bred for nearly one year under the indoor dark condition (25 ℃) is used as the target soil-inhabiting termites for each selective feeding experiment.
The selective feeding experiment employs a conventional double selection experiment. As shown in FIG. 1, a colony of Alteroides sanguinea (about 100-. One of the containers B and C was randomly selected to receive 5g of pine wood particles (as a control), while the other container received the same weight of another material (one of fir, bagasse, and St. triphylla). The device was then placed in the dark in the room (25 ℃) and, after one month, the two food materials were removed and weighed. The bait consumption was used as the feed intake for termites to evaluate the trapping effect of the material on termites. The more the material is reduced, the better the trapping effect is; the less the material reduction, the less the trapping effect. Each set of material was set up for 3 replicates (averaged).
As can be seen from fig. 2, the amount of fir wood taken by odontotermes formosanus is substantially equivalent to the amount of pine wood taken by odontotermes formosanus, and there is no significant difference, indicating that odontotermes formosanus does not show obvious feeding preference for the two materials. Likewise, termites also did not show significant feeding preference for esparto grass. However, compared with the pine wood which is eaten at 0.83g, the amount of bagasse eaten by the odontotermes formosanus reaches 1.55g, the feed intake is improved by nearly 1 time, and the feed intake of the two kinds of the odontotermes formosanus has a significant difference (t is less than 0.05), which indicates that the odontotermes formosanus prefers to eat the bagasse instead of the pine wood. Therefore, bagasse is selected as a matrix material of the trapping and killing bag in the invention.
The general method for trapping and killing the termite wrapped matrix is to select one or more than one material and crush the material; then the crushed material is mixed with chemical agents and bagged. Currently, few studies report on the effect of particle size on feeding of termites inhabiting the soil. The invention takes bagasse as a material, and the bagasse is dried, crushed and sieved to obtain four grades of particles with the sizes of 0.25, 0.25-1, 1-2 and 2mm, and the influence of the bagasse with different particle sizes on feeding and distribution of odontotermes formosanus is researched.
The termite selective feeding experiment adopts four-way selection. The procedure was the same as the double selection experiment in example 1, except that: communicated with the container for placing the termite colony are four containers with different directions, which are respectively used for placing bagasse with four particle sizes. In the experiment, four kinds of particles each having a mass of 5g were randomly placed in containers in four directions, respectively, and then the apparatus was placed in a dark room (25 ℃ C.) and the number of termites in the four containers was recorded every 5 days. After one month, the granules were removed and weighed. The experiment was set up in 3 replicates.
As can be seen from Table 1, the feeding of <0.25mm bagasse by odontotermes formosanus is the least, 0.25g, while the feeding of 1-2mm bagasse is the most, up to 0.82g, which is improved by more than 3 times, indicating that odontotermes formosanus prefers to feed bagasse with a particle size of 1-2 mm. It can also be seen from table 1 that the average number of distribution of odontotermes formosanus in the container with 1-2mm particles was the highest, reaching about 13.11, while the number of distribution in the containers with other particles was less than 7.5, with the lowest number of distribution in the container with <0.25mm particles, only about 3.39. From the above results, it can be concluded that bagasse having a particle size of 1 to 2mm has a significant attraction effect on feeding of odontotermes formosanus.
TABLE 1 feeding and distribution of different granular bagasse by odontotermes formosanus (mean. + -. standard error)
Therefore, the bagasse filled in the particles with the particle size of 1-2mm is used as the raw material of the matrix.
Drawings
The following describes embodiments of the present invention in further detail with reference to the accompanying drawings.
FIG. 1 is a selective feeding device for odontotermes formosanus used in the experiment. Placing a nest group of the black-fin soil termites in a container A; the B and C containers were randomly placed with pine (control) and another termite feeding material (cedar, bagasse or pennisetum).
Fig. 2 is a graph of the selective feeding of termites against four matrix materials, termite feeding was measured against cedar wood, bagasse, and pennisetum, respectively, using pine wood particles as a control, indicating a significant difference (t <0.05) from the control.
Detailed Description
The invention will be further illustrated with reference to the following specific examples. It is to be understood that these examples are for illustrative purposes only and are not intended to limit the present invention.