阅读说明：本技术 利用移动终端对目标成分进行检测的方法及系统 (Method and system for detecting target component by using mobile terminal ) 是由 曹振 王静 王淼 金茂俊 金芬 佘永新 于 2019-11-15 设计创作，主要内容包括：本申请提供一种利用移动终端对目标成分进行检测的方法及系统,以及一种对多目标物同时筛查的检测方法。利用移动终端对目标成分检测的方法包括：使用试纸条对目标成分进行检测得到显色的试纸条；将试纸条绕同一圆心等角度排列,得到试纸条排列环；在试纸条排列环上、所述试纸条之外的区域设置有定位标识；使用移动终端对试纸条排列环进行图像采集,并将采集图像上传至数据处理中心；根据定位标识对采集图像进行矫正；确定图像中每个试纸条的个性化标志区和结果显示区的位置；分割每个试纸条中个性化标志区和结果显示区的图像；计算结果显示区的显色结果,并获取目标成分的测试结果；输出目标成分的测试结果并在移动终端上显示。(The application provides a method and a system for detecting target components by using a mobile terminal, and a detection method for simultaneously screening multiple targets. The method for detecting the target component by using the mobile terminal comprises the following steps: detecting a target component by using a test strip to obtain a colored test strip; arranging the test strips around the same circle center at equal angles to obtain a test strip arrangement ring; positioning marks are arranged on the test strip arrangement ring and in the area outside the test strips; the method comprises the following steps of collecting images of a test strip arrangement ring by using a mobile terminal, and uploading the collected images to a data processing center; correcting the collected image according to the positioning identifier; determining the positions of the personalized mark area and the result display area of each test strip in the image; dividing images of the personalized mark area and the result display area in each test strip; calculating a color development result of the result display area, and obtaining a test result of the target component; and outputting and displaying the test result of the target component on the mobile terminal.)

1. A method for detecting a target component by using a mobile terminal is characterized by comprising the following steps:

detecting the sample solution to be detected by using a test strip to obtain a colored test strip; the test strip at least comprises a personalized mark area and a result display area;

arranging the test strips around the same circle center at equal angles to obtain a test strip arrangement ring; a positioning mark is arranged on the test strip arrangement ring and in the area outside the test strips;

the method comprises the following steps of collecting images of a test strip arrangement ring by using a mobile terminal, and uploading the collected images to a data processing center;

identifying a positioning identifier in the acquired image, correcting the acquired image according to the positioning identifier, and segmenting images of the personalized mark area and the result display area in each test strip;

calculating a color development result of the result display area, and comparing the color development result with a standard color development result in a standard database which is established in advance and matched with the identity information in the personalized mark area to obtain a test result of the target component;

and outputting and displaying the test result of the target component on the mobile terminal.

2. The method of claim 1, wherein said rectifying the acquired image according to the location indicator comprises:

correcting a shooting angle and correcting a position deviation;

preferably, the correcting the acquired image according to the positioning identifier includes: and identifying the position and/or the shape of the positioning mark, and correcting the shooting angle of the acquired image and/or correcting the position deviation of the acquired image according to the position and/or the shape of the positioning mark.

3. The method according to claim 1 or 2, wherein the positioning mark has a predetermined shape, and is disposed at a predetermined position;

preferably, the positioning mark has a shape with distinct vertices and straight sides.

4. The method of any one of claims 1 to 3, wherein the dipstick alignment ring is obtained by restraining the dipstick on a test plate; the positioning mark is a mark block arranged on the detection disc, and/or the positioning mark is the edge of the detection disc;

preferably, the mark blocks are a distinguishing mark block and a contrasting mark block disposed on the detection disc.

5. The method of any one of claims 1 to 4, wherein the identifying the positioning identifier in the captured image and correcting the captured image according to the positioning identifier, and the segmenting the image of the personalized mark area and the result display area in each test strip comprises:

identifying a positioning identifier in the acquired image, correcting the acquired image according to the positioning identifier, determining the position of an individualized mark area and a result display area of each test strip in the corrected image according to a preset position relation between the positioning identifier and the test strips, and segmenting the image according to the determined positions of the individualized mark area and the result display area of each test strip to obtain segmented images of the individualized mark area and the result display area in each test strip;

preferably, the recognizing the positioning identifier in the collected image, correcting the collected image according to the positioning identifier, and segmenting the image of the personalized mark area and the result display area in each test strip includes:

identifying a mark block in the acquired image, correcting the acquired image according to the mark block, determining the position of an individualized mark area and a result display area of each test strip in the corrected image according to a preset position relation between the mark block and the test strips, and segmenting the image according to the determined positions of the individualized mark area and the result display area of each test strip to obtain segmented images of the individualized mark area and the result display area in each test strip;

preferably, the recognizing the positioning identifier in the collected image, correcting the collected image according to the positioning identifier, and segmenting the image of the personalized mark area and the result display area in each test strip includes:

recognizing the edge of a detection disc in the acquired image, correcting the acquired image according to the edge of the detection disc, determining the position of an individualized mark area and a result display area of each test strip in the corrected image according to a preset position relation between the edge of the detection disc and the test strips, and segmenting the image according to the determined positions of the individualized mark area and the result display area of each test strip to obtain segmented images of the individualized mark area and the result display area in each test strip;

preferably, the recognizing the positioning identifier in the collected image, correcting the collected image according to the positioning identifier, and segmenting the image of the personalized mark area and the result display area in each test strip includes:

identifying the mark block and the edge of the detection disc in the acquired image, correcting the acquired image according to the mark block and the edge of the detection disc, determining the position of the personalized mark area and the result display area of each test strip in the corrected image according to the common information of the mark block and the edge of the detection disc and the preset position relationship between the mark block and the edge of the detection disc and the test strips, and segmenting the image according to the determined positions of the personalized mark area and the result display area of each test strip to obtain the segmented image of the personalized mark area and the result display area in each test strip.

6. The method of any one of claims 1 to 5, wherein the result display area in the test strip comprises a T line and a C line;

the color development result is the ratio of the chromatic value of the T-line image to the chromatic value of the C-line image; or the color development result is a chromatic value of the T-line image, and the chromatic value comprises an RGB value, a gray value or a photometric value.

7. The method as claimed in claim 6, wherein calculating the ratio of the chrominance values of the T-line image to the chrominance values of the C-line image as the color result comprises:

taking the ratio of the colorimetric value of the T line image relative to the background color to the colorimetric value of the C line image relative to the background color as the color development result;

preferably, the selection area of the background color is located near a T line or a C line in the result display area;

preferably, the selected area of the background color is located between the line T and the line C.

8. The method of any one of claims 1 to 7, wherein the detection of the target component by using the test strip comprises:

respectively placing the test strips in a plurality of sample accommodating cavities containing target components, wherein the volumes of sample liquids in the sample accommodating cavities are the same; the test strip is put into the sample containing cavity at the same inclination angle and for the same time.

9. The method of any of claims 4 to 8, further comprising: calibrating the sequence of the test strips in the test strip arrangement ring through the marking block;

preferably, one marker block which can be distinguished from the other marker blocks is taken as an order starting point, and the test strips in the test strip arrangement ring are sequentially ordered in a clockwise direction or a counterclockwise direction.

10. The method according to any one of claims 1 to 9, wherein the pixels of the image capturing device of the mobile terminal are at least 800 ten thousand.

11. A method for synchronously detecting multiple targets is characterized by comprising the following steps:

liquid sample liquid to be detected is contained in a plurality of sample containing cavities;

carrying out chromatographic detection on the sample in the sample accommodating cavity by using the test strip; the test strip at least comprises a personalized mark area and a result display area;

placing the test strips after chromatography detection on a detection disc provided with a mark block, wherein the detection disc can enable the test strips to be arranged around the center of the detection disc at equal angles in the same annular area;

acquiring images of the detection disc by using a mobile terminal;

uploading the acquired image to a data processing center for data processing to obtain a detection result of the target object;

and returning the detection result of the target object to the mobile terminal.

12. A method for synchronously detecting multiple targets is characterized by comprising the following steps:

liquid sample liquid to be detected is contained in a plurality of sample containing cavities with liquid dripping pipelines at the bottoms;

placing test strips on a test tray, wherein the test strips are equiangularly arranged around the center of the test tray in a circular ring area of the test tray; the test strip at least comprises a personalized mark area, a result display area and a liquid suction area;

correspondingly placing the dropping liquid pipelines of the plurality of sample accommodating cavities above the liquid suction area of each test strip so that the test strips can carry out chromatography detection on the liquid samples in the sample accommodating cavities;

after the chromatography of the test strip is finished, the mobile terminal is used for carrying out image acquisition on the detection disc and the test strip on the detection disc;

uploading the acquired image to a data processing center for data processing to obtain a detection result of the target object;

and returning the detection result of the target object to the mobile terminal.

13. The method according to claim 11 or 12, wherein the upper surface of the test tray is provided with a marking block for positioning and marking the test strip in the image acquisition process;

preferably, the marking block comprises a distinguishing marking block and a contrast marking block; the distinguishing mark block is used for distinguishing from the comparison mark block;

preferably, the total number of the distinguishing mark blocks and the comparison mark blocks is not less than three;

preferably, the shape of the marker block includes at least a vertex and a straight edge;

preferably, the mark blocks are one blue square color block and three green square color blocks, or the mark blocks are one green square color block and three blue square color blocks.

14. The method of any one of claims 11 to 13, wherein the upper surface of the test plate is a regular polygon;

preferably, the upper surface of the detection disc is a neutral color surface;

preferably, the upper surface of the detection disc is a diffuse reflection surface.

15. The method according to any one of claims 11 to 14, wherein the sample liquid amounts in the plurality of sample-receiving chambers are the same when the test strip is used for performing chromatographic detection on the samples in the sample-receiving chambers; the test strip is contacted with the liquid sample in the sample accommodating cavity for the same time;

optionally, when the test strip is inserted into the sample accommodating cavity, the inclination angle of the test strip inserted into the sample accommodating cavity is the same;

optionally, the dropping circuit is placed at the same position above the liquid sucking area of the test strip when the test strip is positioned at the bottom of the sample holding cavity.

16. The method of any one of claims 1 to 15, wherein an upper surface of the strip is flush with an upper surface of the test tray after the strip is placed on the test tray;

preferably, an upper surface of the result display area is flush with an upper surface of the test tray.

17. The method according to any one of claims 11 to 16, wherein the image capturing device of the mobile terminal has at least 800 ten thousand pixels.

18. The method of any one of claims 11 to 17, wherein a strip holding groove recessed downward from an upper surface of the test tray is provided on the test tray for holding the strip;

preferably, a separation block for separating the lower surface of the test strip from the bottom of the test strip containing groove is arranged at the bottom of the test strip containing groove;

preferably, when the test strip is placed in the test strip containing groove, the personalized mark area is close to the circle center of the test tray, and the frame of the area containing the test strip in the test strip containing groove is matched with the shape of the test strip.

19. A system for detecting a target component using a mobile terminal, comprising:

the sample liquid detection device comprises a test strip and a detection disc; the test strip at least comprises a personalized mark area and a result display area; the test tray is used for limiting the test strips to be arranged around the same circle center at equal angles so as to obtain a test strip arrangement ring; the detection disc is also provided with a positioning mark for positioning the position of the test paper strip in the test paper strip arrangement ring after image acquisition;

the image acquisition device is arranged on the mobile terminal and is used for acquiring images of the test strip arrangement rings;

the data processing device is used for carrying out data processing on the acquired image and obtaining a test result of the target component;

and the test result receiving device is used for receiving the test result of the target component and is arranged on the mobile terminal.

20. A system according to claim 19, wherein the system is configured to perform a fast detection method according to any one of claims 1-10; or the system is used to perform a fast detection method as claimed in any one of claims 11 to 18.

21. The system of claim 19, wherein the data processing device comprises:

the image correction unit is used for identifying the positioning identifier in the acquired image and correcting the acquired image according to the positioning identifier;

the image segmentation unit is used for segmenting images of the personalized mark area and the result display area in each test strip;

and the calculating unit is used for calculating the color development result of the result display area, comparing the color development result with a standard color development result in a standard database which is established in advance and matched with the identity information in the personalized mark area, and acquiring the test result of the target component.

22. The system according to any of claims 19 to 21, characterized in that said data processing means are provided on said mobile terminal; or the data processing device is arranged on a remote terminal which is in communication connection with the mobile terminal.

Technical Field

The application relates to the technical field of immunological detection, in particular to a method and a system for detecting target components by using a mobile terminal, and a detection method for simultaneously screening multiple targets.

Background

At present, the target is usually detected by immunochromatography, and immunochromatography is usually performed by a test strip. The test paper strip has different types and different reading modes, improves the use difficulty of the test paper strip in non-professional people, and cannot meet the requirement of people on real-time detection of the target object.

Furthermore, even for professionals, different concentrations of target can cause different shades of test lines on the strip. Qualitative or quantitative detection of a target object is determined by observing a color development result of the test strip by naked eyes, and the detection result is inaccurate due to the influence of personal subjective factors of operators.

Disclosure of Invention

The embodiment of the application aims to provide a method for detecting target components by using a mobile terminal, which can simultaneously detect multiple target components by using the mobile terminal, is not influenced by factors such as the type of the mobile terminal and the inclination of a shooting angle, and has the characteristics of low requirement on the professional degree of an operator and capability of qualitatively and/or quantitatively detecting a target object under conventional ambient light conditions at any time and any place.

In a first aspect, an embodiment of the present application provides a method for detecting a target component by using a mobile terminal, including:

detecting the sample solution to be detected by using a test strip to obtain a colored test strip; the test strip at least comprises a personalized mark area and a result display area;

arranging the test strips around the same circle center at equal angles to obtain a test strip arrangement ring; a positioning mark is arranged on the test strip arrangement ring and in the area outside the test strips;

the method comprises the following steps of collecting images of a test strip arrangement ring by using a mobile terminal, and uploading the collected images to a data processing center;

identifying a positioning identifier in the acquired image, correcting the acquired image according to the positioning identifier, and segmenting images of the personalized mark area and the result display area in each test strip;

calculating a color development result of the result display area, and comparing the color development result with a standard color development result in a standard database which is established in advance and matched with the identity information in the personalized mark area to obtain a test result of the target component;

and outputting and displaying the test result of the target component on the mobile terminal.

In the implementation process, each test strip is provided with an individual mark area, and the individual mark area is provided with identity information for identifying the type of the test strip where the individual mark area is located. The test strips are arranged around the same circle center at equal angles, images of different test strips have obvious difference due to the fact that the test strips are arranged in a large or small mode when the test strips are shot relative to the combination of the test strips in the non-circumferential array, and the circumferential arrangement in the application enables the image distortion of the collected test strips to tend to be the same. The acquired image of the test strip is corrected through the positioning identification, and image distortion caused by shaking, angle inclination and the like in the image acquisition process is eliminated. The accurate positions of the personalized mark area and the result display area in each test strip image are obtained according to the preset position relation under the condition that the test strip images are almost undistorted, so that the higher matching degree of the segmented result display area and the actual result display area is ensured in the image processing process of the test strip result display area, and a more accurate detection result can be obtained after the comparison with the matched standard numerical value. Therefore, the method has low requirement on the professional degree of operators, can be used for qualitatively and/or quantitatively detecting the target object under the conventional ambient light condition at any time and any place, and has high accuracy of the detection result.

In a possible implementation manner, the rectifying the acquired image according to the positioning identifier includes:

correction of a shooting angle and correction of a positional deviation.

In the implementation process, the positions, the sizes and the placing directions of objects such as test strips, positioning marks and the like in the acquired image are the same as or have a set proportional relation with the positions, the sizes and the placing directions of actual articles by correcting the acquired image, so that the images and the actual articles can be amplified or reduced in the same or equal proportion.

In a possible implementation manner, the rectifying the acquired image according to the positioning identifier includes: and recognizing the position and/or the shape of the positioning mark, and correcting the shooting angle of the acquired image and/or correcting the position deviation of the acquired image.

The positioning mark has characteristics (such as shape, boundary, position, size, color and the like) which can be obviously distinguished from the periphery, and the characteristics of the positioning mark are identified and matched with the characteristics of the preset mark of the data processing center, so that the image is corrected, the correction process is more accurate, and the algorithm is simpler. Compared with the method for directly carrying out edge recognition on the test strip (or the color development area), the method has the advantages that the characteristics (particularly the boundary) of the positioning mark are clearer, the recognition is more accurate, and the correction result is more accurate, so that the accuracy of the color development result recognition is improved, and the reliability of the detection result is guaranteed.

In a possible implementation manner, the positioning mark has a predetermined shape and is arranged at a predetermined position;

the positioning mark has a preset shape and a preset position, and when the image is identified, the data processing center can match the shape and the position of the positioning mark of the collected image according to the preset shape and the preset position, so that the correction of the collected image is realized.

In one possible implementation, the positioning mark has a shape with distinct vertices and straight sides.

In the process of identifying the positioning marks, the obvious vertexes and straight edges are easier to identify and more accurate to identify, and the accuracy of image correction can be improved.

In one possible implementation, the test strip arrangement ring is obtained by limiting the test strips on a test plate; the positioning mark is a mark block arranged on the detection disc, and/or the positioning mark is the edge of the detection disc;

the positioning marks are marks capable of positioning the test strip in the data acquisition process, and the positioning marks can be definite marking blocks or certain structural features (such as the edge of a test tray) of the test tray for containing the test strip.

Preferably, the mark blocks are a distinguishing mark block and a contrasting mark block disposed on the detection disc. The distinguishing mark block and the comparison mark block are used for marking the placing sequence, the position and the like of the test strip.

In a possible implementation manner, the recognizing a positioning identifier in the acquired image, and correcting the acquired image according to the positioning identifier, and the segmenting the image of the personalized mark area and the result display area in each test strip includes:

identifying a positioning identifier in the acquired image, correcting the acquired image according to the positioning identifier, determining the position of an individualized mark area and a result display area of each test strip in the corrected image according to a preset position relation between the positioning identifier and the test strips, and segmenting the image according to the determined positions of the individualized mark area and the result display area of each test strip to obtain segmented images of the individualized mark area and the result display area in each test strip;

in another possible implementation manner, the identifying a positioning identifier in the acquired image, correcting the acquired image according to the positioning identifier, and segmenting the image of the personalized mark area and the result display area in each test strip includes:

identifying a mark block in the acquired image, correcting the acquired image according to the mark block, determining the position of an individualized mark area and a result display area of each test strip in the corrected image according to a preset position relation between the mark block and the test strips, and segmenting the image according to the determined positions of the individualized mark area and the result display area of each test strip to obtain segmented images of the individualized mark area and the result display area in each test strip;

in another possible implementation manner, the identifying a positioning identifier in the acquired image, correcting the acquired image according to the positioning identifier, and segmenting the image of the personalized mark area and the result display area in each test strip includes:

recognizing the edge of a detection disc in the acquired image, correcting the acquired image according to the edge of the detection disc, determining the position of an individualized mark area and a result display area of each test strip in the corrected image according to a preset position relation between the edge of the detection disc and the test strips, and segmenting the image according to the determined positions of the individualized mark area and the result display area of each test strip to obtain segmented images of the individualized mark area and the result display area in each test strip;

in another possible implementation manner, the identifying a positioning identifier in the acquired image, correcting the acquired image according to the positioning identifier, and segmenting the image of the personalized mark area and the result display area in each test strip includes:

identifying the mark block and the edge of the detection disc in the acquired image, correcting the acquired image according to the mark block and the edge of the detection disc, determining the position of the personalized mark area and the result display area of each test strip in the corrected image according to the common information of the mark block and the edge of the detection disc and the preset position relationship between the mark block and the edge of the detection disc and the test strips, and segmenting the image according to the determined positions of the personalized mark area and the result display area of each test strip to obtain the segmented image of the personalized mark area and the result display area in each test strip.

The implementation manner of recognizing the positioning identifier in the acquired image, correcting the acquired image according to the positioning identifier, and segmenting the image of the personalized mark area and the result display area in each test strip can be understood as follows: the correction of the collected image can be realized only by the marking block, can also be realized only by the edge of the detection disc, and can be realized by the marking block and the edge of the detection disc. In the mode of realizing through mark piece and detection dish edge, the correction result of mark piece and the detection result of detection dish edge can proofread each other to improve the accuracy of image correction, improve the discernment precision and the degree of accuracy of color development district, improve the reading accuracy of the color development result of color development district.

In one possible implementation manner, the result display area in the test strip comprises a T line and a C line;

the color development result is the ratio of the chromatic value of the T-line image to the chromatic value of the C-line image; or the color development result is a chromatic value of the T-line image, and the chromatic value comprises an RGB value, a gray value or a photometric value.

In a possible implementation manner, when the calculating the color rendering result is a ratio of T-line image chrominance values to C-line image chrominance values, the calculating includes:

taking the ratio of the colorimetric value of the T line image relative to the background color to the colorimetric value of the C line image relative to the background color as the color development result;

preferably, the selection area of the background color is located near a T line or a C line in the result display area;

preferably, the selected area of the background color is located between the line T and the line C.

In a possible implementation manner, when the test strip is used to detect a target component, the method includes:

respectively placing the test strips in a plurality of sample accommodating cavities containing target components, wherein the sample liquid volumes in the sample accommodating cavities are the same; the test strip is put into the sample containing cavity at the same inclination angle and for the same time.

In the implementation process, the test strip is subjected to chromatography detection in sample liquid with the same volume according to the same angle, the chromatography behaviors are consistent, the difference of the color development result caused by operation errors can be reduced, and the reliability of the color development result is improved.

In a possible implementation manner, the method for detecting a target component by using a mobile terminal further includes: calibrating the sequence of the test strips in the test strip arrangement ring through the marking block;

illustratively, the test strips in the test strip arrangement ring are sequentially ordered in a clockwise direction or a counterclockwise direction by taking one marker block which can be distinguished from the rest marker blocks as an order starting point.

In one possible implementation manner, the pixels of the image acquisition device of the mobile terminal are at least 800 ten thousand.

In a second aspect, an embodiment of the present application further provides a method for detecting synchronization of multiple targets, including:

liquid sample liquid to be detected is contained in a plurality of sample containing cavities;

carrying out chromatographic detection on the sample in the sample accommodating cavity by using the test strip; the test strip at least comprises a personalized mark area and a result display area;

placing the test strips after chromatography detection on a detection disc provided with a mark block, wherein the detection disc can enable the test strips to be arranged in the same annular area at equal angles at the center of the detection disc;

the mobile terminal is used for carrying out image acquisition on the detection disc and the test strips on the detection disc;

uploading the acquired image to a data processing center for data processing to obtain a detection result of the target object;

and returning the detection result of the target object to the mobile terminal.

In a third aspect, an embodiment of the present application further provides a method for detecting synchronization of multiple targets, including:

liquid sample liquid to be detected is contained in a plurality of sample containing cavities with liquid dripping pipelines at the bottoms;

placing test strips on a detection disc provided with a marking block, wherein the test strips are arranged around the center of the detection disc at equal angles in an annular area of the detection disc; the test strip at least comprises a personalized mark area, a result display area and a liquid suction area;

correspondingly placing the dropping liquid pipelines of the plurality of sample accommodating cavities above the liquid suction area of each test strip so that the test strips can carry out chromatography detection on the liquid samples in the sample accommodating cavities;

after the chromatography of the test strip is finished, the mobile terminal is used for carrying out image acquisition on the detection disc and the test strip on the detection disc;

uploading the acquired image to a data processing center for data processing to obtain a detection result of the target object;

and returning the detection result of the target object to the mobile terminal.

In a possible detection mode, a marking block is arranged on the upper surface of the detection disc and used for positioning and marking the test strip in the image acquisition and processing process.

The marking blocks can also play a role in correcting the image, and the edge of the detection disc and the detection blocks are used for detection, so that the accuracy of image correction can be improved.

Preferably, the marking block comprises a distinguishing marking block and a contrast marking block; the distinguishing mark block is used for distinguishing from the comparison mark block.

Preferably, the total number of the distinguishing mark blocks and the comparison mark blocks is not less than three.

The distinguishing mark block and the comparison mark block can realize the marking of the arrangement sequence of the test paper strips. More than three marking blocks can better correct the shooting angle.

Preferably, the shape of the marker block includes at least a vertex and a straight edge.

The mark blocks with the top points and the straight edges are easier to identify and position, thereby realizing accurate correction of the image.

Preferably, the mark blocks are one blue square color block and three green square color blocks, or the mark blocks are one green square color block and three blue square color blocks.

Blue, green and yellow are stable colors, and are not easily influenced by ambient light when the edge of the mark is identified, so that the identification result is more accurate.

In one specific implementation manner, the upper surface of the detection disc is a regular polygon.

The regular polygon has clear vertexes and edges, is easier to recognize during image processing, and is matched with the shape preset by the data processing center, namely the image correction is more accurate.

Preferably, the upper surface of the test tray is a neutral color surface.

Preferably, the upper surface of the detection disc is a diffuse reflection surface.

The neutral color surface is less influenced by an external light source, the color interference of reflected light to the color development area is small, and the color development result is read more accurately when data is read. The diffuse reflection surface does not generate light spots reflected by the light source, so that the interference on image acquisition is avoided.

In a possible implementation manner, when the test strip performs chromatographic detection on the samples in the sample accommodating cavities, the amounts of the sample liquid in the sample accommodating cavities are the same; the test strip is in contact with the liquid sample in the sample containing cavity for the same time.

The same test condition can reduce the error of the color development result caused by the operation error.

Optionally, when the test strip is inserted into the sample accommodating cavity, the inclination angle of the test strip inserted into the sample accommodating cavity is the same.

For the step of carrying out the chromatographic detection of the sample solution by the test strip, and then arranging the test strips into a circumferential mode, the insertion angle of the test strips is also a variable factor of the operation, and the same insertion angle of the test strips can also reduce the result display error caused by the operation error.

Optionally, the dropping circuit is placed at the same position above the liquid sucking area of the test strip when the test strip is positioned at the bottom of the sample holding cavity.

For the mode of directly arranging the test strips into a circumferential mode and carrying out chromatographic detection on the test strips by the sample liquid in the sample accommodating cavity in a liquid leakage mode, the contact position of the sample liquid on the test strips is also a variable factor of operation, and the same contact position of the sample liquid can also reduce result display errors caused by operation errors.

In one possible implementation, after the test strip is placed on the test tray, the upper surface of the test strip is flush with the upper surface of the test tray.

Preferably, an upper surface of the result display area is flush with an upper surface of the test tray.

The upper surface of the test strip (especially the upper surface of the test strip result display area) is flush with the upper surface of the detection disc, so that shadows caused by light source irradiation can be reduced, the chroma identification accuracy of a color development area in the data processing process is improved, and the accuracy of a detection result is improved.

In one possible implementation manner, the pixels of the image acquisition device of the mobile terminal are at least 800 ten thousand.

The higher the pixel is, the higher the identification precision of the positioning mark is, the higher the image correction accuracy is, the more accurate the color development region segmentation is, the more accurate the color development result identification is, and the more reliable the detection result is.

In a possible implementation manner, a test strip containing groove which is recessed downwards from the upper surface of the detection tray is arranged on the detection tray and is used for containing the test strip.

The test strip holds the groove and can play and carry out spacing effect to the test strip, avoids the test strip because place the angle scheduling problem and slide etc. appear.

Preferably, a separation block for separating the lower surface of the test strip from the bottom of the test strip containing groove is arranged at the bottom of the test strip containing groove.

The spacer block can hold tank bottom separation with test paper strip and test paper strip, prevents that the sample liquid on the test paper strip from polluting the test paper strip and holding the groove, reduces the sample pollution when continuous sample detects, improves the result accuracy.

Preferably, when the test strip is placed in the test strip containing groove, the personalized mark area is close to the circle center of the test tray, and the frame of the area containing the test strip in the test strip containing groove is matched with the shape of the test strip.

The frame of the area for containing the test strip in the test strip containing groove is matched with the shape of the test strip, so that the gap between the test strip and the test strip containing groove can be reduced, and the situation that the selection of a standard database is influenced by a part of the personalized mark which is identified as a personalized mark area by a data processing center is avoided; meanwhile, the interference of the gap shadow on the identification of the T line and the C line in the result color display area can be avoided.

In a fourth aspect, an embodiment of the present application further provides a system for detecting a target component by using a mobile terminal, including:

the sample liquid detection device comprises a test strip and a detection disc; the test strip at least comprises a personalized mark area and a result display area; the test tray is used for limiting the test strips to be arranged around the same circle center at equal angles so as to obtain a test strip arrangement ring; the detection disc is also provided with a positioning mark for positioning the position of the test paper strip in the test paper strip arrangement ring during image acquisition;

the image acquisition device is arranged on the mobile terminal and is used for acquiring images of the test strip arrangement rings;

the data processing device is used for carrying out data processing on the acquired image and obtaining a test result of the target component;

and the test result receiving device is used for receiving the test result of the target component and is arranged on the mobile terminal.

The system for detecting the target component by using the mobile terminal is simple in carrying device, the sample liquid detection device is small in size and easy to carry, the image acquisition device, the data processing device and the test result receiving device can be carried on the mobile terminal or a cloud terminal, the operation is simple, the operation difficulty is reduced, and the popularization of the target component detection method is made possible. In addition, the system for detecting the target component by using the mobile terminal can realize multi-channel detection, and the detection result is reliable.

In one possible implementation, the system is used for performing the method for detecting the target component by using the mobile terminal, which is one of the purposes; or the system is used for carrying out the method for synchronously detecting the multiple targets.

The system for detecting the target component by using the mobile terminal can perform detection in different modes, and is preferably used for performing one of purposes, namely, a method for detecting the target component by using the mobile terminal; or the method for synchronously detecting the multiple targets is carried out.

In one possible implementation, the data processing apparatus includes:

the image correction unit is used for identifying the positioning identifier in the acquired image and correcting the acquired image according to the positioning identifier;

the image segmentation unit is used for segmenting images of the personalized mark area and the result display area in each test strip;

and the calculating unit is used for calculating the color development result of the result display area, comparing the color development result with a standard color development result in a standard database which is established in advance and matched with the identity information, and acquiring the test result of the target component.

Each part of the data processing device can correct the acquired images, and the problems that the acquired images of the mobile terminal are different and the identification result is inaccurate are solved; the accurate segmentation of the required areas (the personalized mark area and the result display area) is realized by combining the segmentation unit; furthermore, the reading of the color development result of the accurately divided color development area is realized by combining with a computing unit.

The data processing device is one implementation of the system data processing device for detecting a target component by using a mobile terminal provided by the present application, and a person skilled in the art can select any implementation of a data processing center capable of performing data processing on an image captured by the mobile terminal to obtain a reliable test result.

In a possible implementation manner, the data processing device is arranged on the mobile terminal; or the data processing device is arranged on a remote terminal which is in communication connection with the mobile terminal.

Compared with the prior art, the method has the following beneficial effects:

(1) the application provides a method for detecting a target component by using a mobile terminal, which utilizes a test strip arrangement mode of a circumferential array, and segments a required test strip area to obtain a chromatic value by identifying and positioning marks in an image identification process, so that the chromatic value is compared with the chromatic value of a corresponding standard database to obtain a test result. The method omits the process of directly identifying the color development area, and reduces the disadvantage of larger identification error of the width direction of the test strip caused by inaccurate edge identification; meanwhile, the processing process can realize multi-channel detection, and the detection of the test strip of each channel can output a reliable detection result.

(2) The application provides a method for synchronously detecting multiple targets, only chromatography detection of test strips, test strip placement, mobile terminal photographing and uploading to a system are needed, so that test results can be obtained, the process is simple, the operation difficulty is low, the popularization is easy, and accurate measurement is realized for target component detection of a sample liquid; meanwhile, the detection of the multi-channel test strip can be realized by utilizing the mobile terminal.

(3) The application also provides a system for detecting the target components by using the mobile terminal, only the sample liquid detection device is used, and the mobile terminal and the data processing device (which can be at the cloud end or the mobile terminal) which are provided with the image acquisition device and the test result receiving device are used for detecting the target components, so that the device is easy to carry, can be used in families, is easy to popularize in a market mode, and is reliable in test results.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.

Fig. 1 is a flowchart illustrating a method for detecting a target component by using a mobile terminal according to an embodiment of the present disclosure;

FIG. 2 is a schematic diagram of a test tray and test strips arranged on the test tray according to one embodiment;

fig. 3 is a flowchart illustrating a method for detecting synchronization of multiple targets according to an embodiment of the present disclosure;

fig. 4 is a flowchart illustrating a method for detecting synchronization of multiple targets according to an embodiment of the present disclosure;

FIG. 5 is a schematic diagram illustrating the construction of multiple sample receiving chambers according to one embodiment;

FIG. 6 is a bottom view of a multiple sample receiving chamber construction according to one embodiment;

fig. 7 is a schematic diagram illustrating a system for detecting a target component by using a mobile terminal according to an embodiment.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

The method and the system are realized by an immunological detection technology, so that the method and the system in the embodiment of the application both adopt a test strip to carry out immunological detection.

The test paper strip in this application embodiment is at least including the sample pad that is used for dropwise add sample liquid, and sample pad dislocation stack colloidal gold fills up, the colloidal gold fill up with the crisscross place of sample pad sets up the MAX line, colloidal gold pad other end overlap joint nitrocellulose membrane cellulose nitrate other end overlap joint absorbent material. And the nitrocellulose membrane is provided with a T line (detection line) and a C line (quality control line). Used for carrying out color reaction on the target in the sample liquid. In the test strip, the T line and the C line can present different colors according to the content of the target object in the sample liquid, and the content of the target object in the sample liquid is obtained by reading the color ratio of the T line and the C line of the test strip. In the test strip, the sample pad and the colloidal gold part can cover the personalized mark to obtain a personalized mark area, and the water absorbing material part can also cover the personalized mark to obtain the personalized mark area: the area of the nitrocellulose membrane is the result color zone.

The test strip in the embodiment of the application at least comprises a personalized mark area, a result display area and a capillary area. The capillary area is used for adsorbing the liquid sample to the test strip and enabling the liquid sample to enter the area provided with the colloidal gold layer to perform color development reaction, and the test result after color development is presented in the result display area. Generally, a T line (detection line) and a C line (quality control line) are arranged in a result display area of the test strip, and a detection result can be obtained only through the color development reaction of the T line or can be obtained through the combination of the color development results of the T line and the C line. The personalized mark area is used for setting identity information, and the identity information comprises but is not limited to information such as the type, brand, factory batch number and the like of the test strip. Meanwhile, the identification information is identifiable information, such as a two-dimensional code, a bar code, and the like. In the embodiment of the present application, the personalized mark area may be disposed in any area except the result display area, and the personalized mark area does not block the result display area.

Before target component detection is carried out, a standard database needs to be established in advance for each type of test strip, and a mapping relation is established between the color development reaction result and the content of the target component in the standard database.

The mapping relationship between the color reaction result and the content of the target component is typically, but not by way of limitation, an external standard method, that is, a sample solution of the target component with a known concentration is selected to perform chromatography detection on a test strip with a specific personalized marker, and then the method provided by the present application is used to perform correspondence between the color reaction result and the concentration of the chromatography detection test strip, specifically, the sample solution of the target component with a known concentration is detected by any one of operations S101 to S106, S301 to S306, or S401 to S406, so as to obtain a color reaction result matched with the concentration of the target component.

The following is a detailed description of how detection of the target component is achieved.

Fig. 1 is a flowchart illustrating detection of a target component by a mobile terminal according to an embodiment of the present disclosure. Referring to fig. 1, the method for detecting a target component by using a mobile terminal includes the following steps:

s101: and detecting the target component by using the test strip to obtain the chromogenic test strip.

In one embodiment, the detection of the target component using the test strip includes detection of the corresponding target using different types of test strips, for example, a urine sample using a urine test strip, a blood sample using a blood test strip, etc.; the sample liquid can also be the same, but a plurality of components in the sample liquid are screened, for example, a plurality of pesticide residues, mycotoxins, environmental pollutants and the like in the same tea sample liquid are screened.

In another embodiment, the detection of the target component using the test strips comprises the detection of the same target from different samples using the same type of test strip. For example, tebuconazole (one of pesticide residues) in different tea water is detected.

It should be noted that the types of test strips in the present application include, but are not limited to, pesticide residue component test strips, various test strips used in the medical field, test strips for environmental pollution component test strips, and the like.

S102: arranging the test strips around the same circle center at equal angles to obtain a test strip arrangement ring; and a positioning mark is arranged on the test strip arrangement ring and in the area outside the test strips.

In one possible implementation, the strip alignment ring is obtained by restraining the strip on a test tray. Fig. 2 is a schematic structural diagram illustrating a test tray and test strips arranged on the test tray according to an embodiment. Referring to FIG. 2, the test strips 100 are equiangularly arranged around the center of the test tray 200.

In one embodiment, the test strip is limited on the test tray, a test strip containing groove for containing the test strip is arranged on the test tray, and when the test strip is limited in the test strip containing groove, the upper surface of the test strip is preferably flush with the upper surface of the test tray.

It should be noted that it is only exemplary to adopt the test strip holding groove to limit the test strip, and the test strip still can be limited to the test strip through other limit structure, for example, limit the test strip through any one or the combination of at least two of structures such as cylindricality or polygon prism shape stopper, limit baffle, limit slot, etc. this application does not specifically limit to limit structure, and all can be with the test strip realize on the detection dish equiangularly arrange the structure of forming the test strip range ring and all fall into the protective range of this application.

In one possible implementation, the positioning marks are mark blocks provided on the test tray 200, see fig. 2, which are a distinguishing mark block 300 and a contrast mark block 400 provided on the test tray 200. The distinguishing mark block 300 is used to distinguish from the comparison mark block 400. In one possible implementation, the shape of the marker block includes at least a vertex and a straight edge. Referring to fig. 2, the identification block is shaped as a square block. The upper surface of the identification block is square. It should be noted that the shape of the upper surface of the identification block may also be other regular polygons or regular patterns with vertices and straight sides, and the shape of the upper surface of the identification block is not specifically limited in this application, but is preferably a regular pattern including vertices and straight sides, such as a polygon or a regular polygon, a pentagram, and the like.

In one embodiment of the flag, the top surfaces of the distinctive mark blocks 300 and the comparative mark blocks 400 are equal in area, and the distinctive mark blocks 300 and the comparative mark blocks 400 are collectively arranged in a circumferential array around the center of the test disc 200 and near the outer edge of the test disc.

In a specific embodiment of the mark block, the mark block 300 and the contrast mark block 400 are arranged on the outer edge of the detection disc, so that the mark block is far away from the center of the detection disc as far as possible, the image definition of each collected mark block is higher, and when a shot image is identified, the angle and the position of the picture can be corrected more accurately through the mark block.

In one embodiment of the identification block, the geometric center connecting line of the distinguishing mark block 300 and the comparison mark block 400 encloses a square area, and the center of the square area coincides with the center of the detection disc. The design scheme can simplify the correction algorithm and improve the information accommodation capacity and the processing speed of the data processing center.

In one embodiment of the identification block, the top surface of the distinguishing mark block 300 is a blue square, and the top surface of the comparison mark block 400 is three green squares; or the upper surface of the distinguishing mark block 300 is a green square and the upper surface of the contrast mark block 400 is three blue squares.

In the implementation process, the blue and the green are colors with relatively stable chromaticity, are less influenced by the brightness of the environment light, have higher precision in boundary identification and are beneficial to accurate correction of the collected image.

It should be noted that the schemes shown in the above embodiments for the shape, color, arrangement position, and the like of the identification block are only exemplary, and the shape, color, arrangement position, and the like of the identification block are not specifically limited in the present application, and all the identification features that can correct the image of the test strip to be detected in the collected image, especially the image of the color display area of the test strip, fall within the protection scope of the present application.

In another possible implementation, the location indicator may be an edge of the test tray 200, i.e., six sides of a hexagonal test tray in the test tray 200 shown in fig. 2. In yet another possible implementation, the location indicator may be a combination of both a mark block and an edge of the test tray 200.

It should be noted that the shape of the edge of the test tray 200 is not particularly limited in the present application, but as a preferred embodiment, the edge of the test tray 200 is also a regular pattern including a vertex and a straight edge.

S103: and the mobile terminal is used for collecting images of the test strip arrangement ring and uploading the collected images to the data processing center.

The mobile terminal in the embodiment of the present application includes, but is not limited to, a mobile phone, a notebook, a tablet computer, a POS machine, even a vehicle-mounted computer, and the like. All terminals with image acquisition devices fall within the scope of protection of the present application.

In one possible implementation, the image capture device of the mobile terminal has at least 800 million pixels (e.g., 900, 1000, 1200, 1500, 1600, 1800, 2000, 2300, 2800, 3500, 40000, etc.).

The higher the pixel of the image acquisition equipment of the mobile terminal is, the higher the resolution of the acquired image is, the higher the identification precision of the image is, and the more accurate the detection result obtained according to the image is.

S104: and identifying the positioning identification in the acquired image, correcting the acquired image according to the positioning identification, and segmenting the images of the personalized mark area and the result display area in each test strip.

In a possible implementation manner, after identifying the positioning identifier in the captured image, the captured image is corrected according to the positioning identifier, specifically: and determining the positions of the personalized mark area and the result display area of each test strip in the corrected image according to the preset position relationship between the positioning identifier and the test strips. The preset position relationship between the positioning mark and the test strip is a preset position relationship, or is limited by a preset position relationship between the test strip and a device corresponding to the test strip. Taking the test strip limited on the test tray as an example, the relationship between the position for holding the test strip in the test tray and the positioning mark on the test tray is a fixed relationship, in the image processing process, the relationship between the positioning mark and the test strip is the established position relationship between the test strip in the test tray and the positioning mark, and because of the test strip, the positions of the result display area and the personalized mark area in the test strip are also the established position relationship.

When the positioning mark is a detection block on the detection disc, the method for correcting the collected image comprises the following steps: and determining the positions of the personalized mark area and the result display area of each test strip in the corrected image according to the preset position relationship between the mark block and the test strips.

When the positioning mark is the edge of the detection disc, the method for correcting the collected image comprises the following steps: and determining the positions of the personalized mark area and the result display area of each test strip in the corrected image according to the preset position relationship between the edge of the detection disk and the test strips.

When the positioning mark is a detection block on the detection disc and the edge of the detection disc, the method for correcting the acquired image comprises the following steps: determining the position of the edge of the detection disc according to the preset position relation between the mark block and the edge of the detection disc; and determining the positions of the personalized mark area and the result display area of each test strip in the corrected image according to the preset position relationship between the edge of the detection disk and the test strips.

And after the positions of the personalized mark area and the result display area of each test strip are determined, image segmentation is carried out to obtain segmented images of the personalized mark area and the result display area in each test strip.

It should be noted that, the determination of the positions of the personalized mark area and the result display area through the preset positional relationship is only an example, and the determination of the positions of the personalized mark area and the result display area through which positional relationship is used is not particularly limited in the present application.

S105: and calculating a color development result of the result display area, and comparing the color development result with a standard color development result in a standard database which is established in advance and matched with the identity information in the personalized mark area to obtain a test result of the target component.

In the embodiment of the application, before detection, a standard database is established for each type of test strip in advance, and in the standard database, a mapping relation is formed between the color development result and the content of a target component. In one possible implementation, the display result generally uses a T/C contrast curve to display the mapping relationship between the color development result and the content of the target component. If the test strip is a vanishing method test strip, a T-line image colorimetric value is usually adopted to display a mapping relation between a color development result and the content of a target component.

And after the color development result is obtained, searching a target concentration value corresponding to the color development result in a T/C comparison curve or a T value straight line graph.

When the ratio result of the T-line image chrominance values and the C-line image chrominance values is used as the color development result, the chrominance values of the T-line image and the C-line image are calculated, wherein the chrominance values include, but are not limited to, RGB values, gray values or luminance values.

In a possible implementation manner, when the calculating the color rendering result is a ratio of the chrominance values of the T-line image to the chrominance values of the C-line image, the calculating includes:

and taking the ratio of the chroma value of the T-line image relative to the background color to the chroma value of the C-line image relative to the background color as the color development result. Preferably, the selection area of the background color is located near a line T or a line C in the result display area. Since the color development result is mainly the chroma ratio of the T line and the C line, if the background color is selected too far away from the T line and the C line, the selection of the background noise is not accurate, and the reading accuracy of the color development result is influenced. The background color is selected near the T line and the C line in the result color display area, on one hand, the materials in the result color display area are basically the same, the reflection behaviors to light rays are consistent, on the other hand, the distance is short, and the background drying effect is better.

More preferably, the selected area of the background color is located between the T line and the C line. The T line and the C line share one background color for drying, and the colorimetric result is more reliable.

In the implementation process, the image in the predetermined area between the T line and the C line is used as the background color, which is advantageous in that the ratio of the chromaticity value of the T line image to the chromaticity value of the C line image is more accurate because the test strip may chromatographically detect other interference colors in the detection process or be influenced by ambient light in the image shooting process, and for the same test strip, the chromatography or the background color of the result display area under the same shooting condition is consistent, so that the chromaticity value of the image in the predetermined area between the T line and the C line of the test strip in the collected image is used as the background color, which is equivalent to filtering the interference of the interference color on the chromaticity values of the T line image and the C line image. Therefore, the calculation method is not influenced by other interference colors, and the calculation of the color rendering result is more accurate.

S106: and outputting and displaying the test result of the target component on the mobile terminal.

For the immunochromatography test strip, after the sample chromatography detection is carried out, the problem of uneven color development of the color development area often occurs, so that the accurate acquisition of the color development result of the color development area becomes an important part for obtaining a reliable detection result, and in the mode of acquiring the color development result through a mobile terminal and data processing, the accurate segmentation of the image of the color development area is a necessary condition for obtaining an accurate color development result. Compared with the test strip combination of the non-circumferential array, the test strips are arranged around the same circle center at equal angles, each test strip can have the same distortion degree after the image is corrected, and the test strip combination of the non-circumferential array still has distortion difference among different test strips after the image is corrected, so that the sizes of color development areas are different, and the reading of color development results is influenced.

Based on the fact that the distortion of each test strip in the corrected image is the same, the accurate positions of the personalized mark area and the result display area in each test strip image are obtained according to the preset position relation, and therefore when the test strips are subjected to image segmentation, the matching degree of the segmented result display area and the actual result display area can be higher, the reading accuracy of the color development result is improved, and the accuracy of the detection result is improved.

Therefore, the purpose of simultaneously detecting the multi-target components by using the mobile terminal is achieved, the operation difficulty is low, the operation equipment is convenient to carry, the detection at any time can be realized, and the detection result is reliable. Meanwhile, multi-channel detection can be performed, and the detection results of the multiple channels are reliable.

The test result of the target component may be output to a mobile terminal for image acquisition, any mobile terminal capable of data transmission with the data processing center, or a data storage unit of the data processing center to store data for retrieval at any time.

According to another aspect of the invention, a method for synchronously detecting multiple targets is also provided. Fig. 3 is a flowchart for detecting synchronization of multiple targets according to an embodiment of the present disclosure. Referring to fig. 3, the method for synchronously detecting multiple targets comprises the following steps:

s301: and liquid sample liquid to be detected is contained in the plurality of sample containing cavities.

In one possible implementation, the plurality of sample receiving chambers are combined into a cylindrical shape. FIG. 5 is a schematic diagram illustrating the assembly of multiple sample-receiving chambers, according to one embodiment. See fig. 5. A plurality of sample receiving chambers 500 are integrally formed to enclose a cylinder having a hollow cavity 510 therein, and each sample receiving chamber 500 includes an outer wall for enclosing an outer edge of the cylinder and an inner wall for enclosing an intermediate cavity. In a preferred embodiment, a bayonet 520 for holding the test strip is provided on each outer wall. The width of the bayonet 520 is the same as or approximately the same as that of the test strip, so that the test strip is just clamped in the bayonet 520, and the test strip is prevented from shaking.

In one possible implementation, the width of the bottom of each sample-receiving chamber 500 or the entire sample-receiving chamber 500 (the width direction is perpendicular to the radius direction of the cylinder) is the same or approximately the same as the width of the test strip.

In the implementation process, when the test strip is inserted into the sample accommodating cavity 500, the test strip is limited by the width limitation, and the test strip is just clamped in the sample accommodating cavity 500. Above-mentioned setting combines with bayonet 520 on the outer wall setting, and then the test paper strip is inserting sample and holding chamber 500 back, and the both ends of test paper strip are all fixed to make the difficult emergence of test paper strip rock, further, the inclination of test paper strip can also carry out fine control.

In one possible implementation, the outer wall of each sample receiving chamber 500 is higher than the inner wall design, i.e. the height of the outer wall of the hollow cavity 510 in the cylinder is lower than the height of the outer wall of the cylinder. When the sample accommodating cavity 500 with the structure is used, only each sample accommodating cavity 500 needs to be filled with a liquid sample, redundant liquid samples can overflow into the hollow cavity 510, the volume of each sample accommodating cavity 500 is the same, the volume of the liquid sample in the sample accommodating cavity 500 is the same, if each sample accommodating cavity 500 is not filled with the liquid sample, the liquid sample with the preset height is contained, the preset height is limited by the user to observe with naked eyes when the user pours, the liquid sample in each sample accommodating cavity 500 is difficult to be ensured to be the same in volume, and the detection result is inaccurate.

S302: carrying out chromatographic detection on the sample in the sample accommodating cavity by using the test strip; the test strip at least comprises a personalized mark area and a result display area.

In one possible implementation, the volumes of the sample liquids in the plurality of sample-containing cavities are the same; the test strip is contacted with the liquid sample in the sample accommodating cavity for the same time; the test strip is inserted into the sample containing cavity at the same inclination angle.

In the implementation process, the detection conditions of each test strip are basically the same during detection, and the aim is to ensure that the detection result of each test strip is stable under almost the same detection conditions, so that the inaccuracy of the detection result caused by different detection conditions is avoided.

S303: the test strip after chromatography detection is placed on a detection disc provided with a mark block, and the detection disc can enable the test strip to be arranged around the center of the detection disc at equal angles in the same annular area.

In a possible implementation manner, a test strip containing groove which is recessed downwards from the upper surface of the detection tray is arranged on the detection tray and is used for containing the test strip.

In one possible implementation, after the test strip is placed on the test tray, the upper surface of the test strip is flush with the upper surface of the test tray.

In the implementation process, the upper surface of the test strip is flush with the upper surface of the detection disc, so that shadows generated during image acquisition caused by the height difference between the test strip and the test strip containing groove or the partial edge of the test strip sheltered by the side wall of the test strip containing groove can be reduced or avoided, and the accuracy of image acquisition is ensured.

In a possible implementation manner, a separation block for separating the lower surface of the test strip from the bottom of the test strip containing groove is arranged at the bottom of the test strip containing groove.

In the implementation process, the isolation block can separate the lower surface of the test strip from the bottom of the test strip containing groove, so that the test strip which chromatographs the sample liquid is prevented from polluting the test strip containing groove, and the detection tray can be reused.

In a possible implementation manner, when the test strip is placed in the test strip containing groove, the personalized mark area is close to the circle center of the test tray, and a frame of an area containing the test strip in the test strip containing groove is matched with the shape of the test strip.

In the implementation process, the personalized mark area is close to the circle center of the detection disc, so that the personalized mark area and the color development area can be conveniently shot in a smaller shooting range, and the acquired image is clearer. The frame of the area for containing the test strip in the test strip containing groove is matched with the shape of the test strip, so that the phenomenon that a gap between the frame of the area for containing the test strip in the test strip containing groove and the edge of the test strip can form a shadow in an acquired image and further influence the accurate reading of the information of the personalized mark area is avoided.

S304: the mobile terminal is used for carrying out image acquisition on the detection disc and the test strips on the detection disc;

s305: uploading the acquired image to a data processing center for data processing to obtain a detection result of the target object;

s306: and returning the detection result of the target object to the mobile terminal.

It should be noted that, the assembly method of the sample holding cavity used in the method for synchronously detecting multiple targets is only exemplary, and the test strip needs to perform chromatography detection in advance, so the method is applied to the sample holding cavity.

According to still another aspect of the present invention, a method for detecting multiple object synchronization is further provided, and fig. 4 is a flowchart for detecting multiple object synchronization according to an embodiment of the present application. Referring to fig. 4, the method for synchronously detecting multiple targets includes the following steps: the method comprises the following steps:

s401: liquid sample liquid to be detected is contained in a plurality of sample containing cavities with liquid dripping pipelines at the bottoms;

in a possible implementation manner, the multiple sample-accommodating chambers in this embodiment are combined into a cylindrical shape, and the structure of the cylindrical sample-accommodating chamber is the same as the assembly manner of the multiple sample-accommodating chambers shown in fig. 5, but different from the structure shown in fig. 5, the outer walls of the multiple sample-accommodating chambers in this embodiment are not provided with bayonets, but are provided with drip pipes at the bottom, and are also provided with clamping posts 540 adapted to the detection plate. FIG. 6 is a bottom view of a multiple sample receiving cavity construction according to one embodiment. See fig. 6. The bottom of each sample receiving chamber is provided with a drip line 530.

S402: placing test strips on a detection disc provided with a marking block, wherein the test strips are arranged around the center of the detection disc at equal angles in an annular area of the detection disc; the test strip at least comprises a personalized mark area, a result display area and a liquid suction area;

in a possible implementation manner, a test strip containing groove which is recessed downwards from the upper surface of the detection tray is arranged on the detection tray and is used for containing the test strip.

In one possible implementation, after the test strip is placed on the test tray, the upper surface of the test strip is flush with the upper surface of the test tray.

In the implementation process, the upper surface of the test strip is flush with the upper surface of the detection disc, so that shadows generated during image acquisition caused by the height difference between the test strip and the test strip containing groove or the partial edge of the test strip sheltered by the side wall of the test strip containing groove can be reduced or avoided, and the accuracy of image acquisition is ensured.

In a possible implementation manner, a separation block for separating the lower surface of the test strip from the bottom of the test strip containing groove is arranged at the bottom of the test strip containing groove.

In the implementation process, the isolation block can separate the lower surface of the test strip from the bottom of the test strip containing groove, so that the test strip which chromatographs the sample liquid is prevented from polluting the test strip containing groove, and the detection tray can be reused.

In a possible implementation manner, when the test strip is placed in the test strip containing groove, the personalized mark area is close to the circle center of the test tray, and a frame of an area containing the test strip in the test strip containing groove is matched with the shape of the test strip.

In the implementation process, the personalized mark area is close to the circle center of the detection disc, so that the personalized mark area and the color development area can be conveniently shot in a smaller shooting range, and the acquired image is clearer. The frame of the area for containing the test strip in the test strip containing groove is matched with the shape of the test strip, so that the phenomenon that a gap between the frame of the area for containing the test strip in the test strip containing groove and the edge of the test strip can form a shadow in an acquired image and further influence the accurate reading of the information of the personalized mark area is avoided.

S403: correspondingly placing the dropping liquid pipelines of the plurality of sample accommodating cavities above the liquid suction area of each test strip so that the test strips can carry out chromatography detection on the liquid samples in the sample accommodating cavities;

in one possible implementation, the volumes of the sample liquids in the plurality of sample-containing cavities are the same; the contact time of the test strip and the liquid sample is the same; the positions of the dropping liquid pipelines above the liquid suction area of the test paper strip are the same.

In the implementation process, the detection conditions of each test strip are basically the same during detection, and the aim is to ensure that the detection result of each test strip is stable under almost the same detection conditions, so that the inaccuracy of the detection result caused by different detection conditions is avoided.

S404: after the chromatography of the test strip is finished, the mobile terminal is used for carrying out image acquisition on the detection disc and the test strip on the detection disc;

s405: uploading the acquired image to a data processing center for data processing to obtain a detection result of the target object;

s406: and returning the detection result of the target object to the mobile terminal.

According to another aspect of the invention, a system for detecting a target component by using a mobile terminal is also provided. Fig. 7 is a schematic structural diagram illustrating a system for detecting a target component by using a mobile terminal according to an embodiment, and referring to fig. 7, the system for detecting a target component by using a mobile terminal includes:

a sample liquid detection device 710 comprising a test strip and a detection tray; the test strip at least comprises a personalized mark area and a result display area; the test tray is used for limiting the test strips to be arranged around the same circle center at equal angles so as to obtain a test strip arrangement ring; the test tray is also provided with a positioning mark for positioning the test paper strip in the test paper strip arrangement ring;

the image acquisition device 720 is arranged on the mobile terminal and is used for acquiring images of the test strip arrangement rings;

the data processing device 730 is used for carrying out data processing on the acquired image and obtaining a test result of the target component;

a test result receiving device 740, configured to receive a test result of the target component, where the test result receiving device is disposed on the mobile terminal.

The system for detecting a target component by using a mobile terminal in this embodiment is used for performing the method for detecting a target component by using a mobile terminal; or the system is used for carrying out a method for synchronously detecting multiple targets.

The image capture device 720 and the test result receiving device 740 may be both provided on the mobile terminal.

In one possible implementation, the data processing apparatus includes:

the image correction unit 731 is used for identifying the positioning identifier in the acquired image and correcting the acquired image according to the positioning identifier;

an image segmentation unit 732 for segmenting the images of the personalized mark area and the result display area in each test strip;

the calculating unit 733 calculates a color development result of the result display area, and compares the color development result with a standard color development result in a standard database which is established in advance and matched with the identity information to obtain a test result of the target component.

In the implementation process, the correction method of the image correction unit refers to a correction method for an image in a method for detecting a target component by using a mobile terminal, and details are not repeated here.

In one possible implementation, the data processing apparatus may be provided on the mobile terminal. In another possible implementation, the data processing apparatus is provided on a remote terminal communicatively connected to the mobile terminal.

Effect verification:

(1) selecting a sample solution of a pesticide tebuconazole, placing the tebuconazole sample solutions with different concentrations in sample containing cavities with the same volume, carrying out chromatography on a test strip by adopting the same detection conditions, and obtaining a chromogenic test strip after the chromatography is finished;

(2) and (2) arranging the test strip after color development in the step (1) in a test strip containing groove of a detection disc according to the requirement of an operation specification, photographing by using a mobile terminal (mobile phone), and uploading the photographed test strip to a data processing center to obtain a T/C value-mobile phone.

(3) And (3) placing the test strip developed in the step (1) into a handheld food safety analyzer GT-710 to obtain a T/C value-instrument.

The test results are shown in table 1:

performing linear fitting on the T/C value-mobile phone and the T/C value-instrument to obtain R of the T/C value-mobile phone and the T/C value-instrument ²Trend changes were highly correlated at 0.9969.

From the test results, the T/C value obtained by shooting the detection device screened by the target object by using the mobile terminal (mobile phone) is higher than that obtained by an instrument. However, it can be seen from the results of the linear fitting, that the variation trends of the two are highly consistent, that is, when independent standard curves are selected, the test results of the target objects are judged to be consistent.

The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.