阅读说明：本技术 一种微生物传感器 (Microbial sensor ) 是由 吴彬 惠光梅 余果 于 2019-09-29 设计创作，主要内容包括：本发明属于微生物检测技术领域,具体的说是一种微生物传感器,包括壳体、信号识别元件、信号接收片、电导基质、绝缘基质、检测电极、反应层、注射针筒、针管固定槽、活塞杆、挤压旋钮、检测触手、电信号盒、注射口和分流管；所述壳体内部固接有信号识别元件；所述信号识别元件一端设置有信号接收片；所述壳体中部设置有针管固定槽；通过设置注射针筒将用于检测的微生物或者微生物衍生品单独存储,使用时单独注入微生物传感器内部进行使用,使得使用的微生物或者微生物衍生品可以单独进行存储,降低了存储难度,同时也可以使用不同的微生物或者微生物衍生品进行检测,使得微生物传感器不再是一次性的消耗品。(The invention belongs to the technical field of microbial detection, and particularly relates to a microbial sensor which comprises a shell, a signal identification element, a signal receiving sheet, a conductive substrate, an insulating substrate, a detection electrode, a reaction layer, an injection needle cylinder, a needle tube fixing groove, a piston rod, an extrusion knob, a detection tentacle, an electric signal box, an injection port and a shunt tube, wherein the signal identification element is arranged on the shell; a signal identification element is fixedly connected inside the shell; one end of the signal identification element is provided with a signal receiving sheet; a needle tube fixing groove is formed in the middle of the shell; microorganism or microorganism derivatives that will be used for detecting through setting up injection cylinder are saved alone, inject microorganism sensor inside during the use and use alone for microorganism or microorganism derivatives that use can save alone, have reduced the storage degree of difficulty, also can use different microorganism or microorganism derivatives to detect simultaneously, make microorganism sensor no longer disposable consumables.)

1. A microbial sensor comprises a housing (1), a signal recognition element (2), a signal receiving sheet (3), a conductive substrate (4), an insulating substrate (5), a reaction layer (6), a reference electrode (7), an auxiliary electrode (8) and a circuit line (9); the method is characterized in that: the device also comprises an injection needle cylinder (10), a needle tube fixing groove (11), a detection tentacle (12), an electric signal box (13), an insulating partition plate (14), a detection socket (15), a signal plug (16), a signal input sheet (17), an injection port (18) and a shunt tube (19); a signal identification element (2) is fixedly connected inside the shell (1); a signal receiving sheet (3) is arranged at one end of the signal identification element (2); a needle tube fixing groove (11) is formed in the middle of the shell (1); the injection syringe (10) is in sliding connection with the needle tube fixing groove (11), and the injection syringe (10) is positioned inside the needle tube fixing groove (11); a detection socket (15) is arranged at one end of the shell (1), when the injection syringe (10) slides to one end of the detection socket (15) in the needle tube fixing groove (11), the needle head part of the injection syringe (10) is positioned in the detection socket (15), and the signal receiving sheets (3) are positioned in the detection socket (15) and below the needle head part of the injection syringe (10); the end part of the signal plug (16) is arranged corresponding to the detection jack (15); an injection port (18) is arranged in the signal plug (16), and when the signal plug (16) is positioned in the detection socket (15), the injection port (18) is sleeved outside the needle head of the injection needle cylinder (10); the signal plug (16) is internally provided with a signal input sheet (17), the signal input sheet (17) is positioned below the injection port (18), and when the signal plug (16) is positioned in the detection socket (15), the signal input sheet (17) is respectively sleeved outside the corresponding signal receiving sheet (3); the other end of the signal plug (16) is fixedly connected to the side surface of the electric signal box (13); an insulating partition plate (14) is fixedly connected to the middle part of the electric signal box (13); one side of the electric signal box (13), which is close to the signal plug (16), is filled with an insulating substrate (5), and the other side of the electric signal box (13) is filled with a conductive substrate (4); the insulating matrix (5) is an organic polymer material; a reference electrode (7) is arranged in the conductive substrate (4), and the reference electrode (7) is communicated with a corresponding signal input sheet (17) through a circuit line (9); an auxiliary electrode (8) is arranged in the conductive substrate (4), the auxiliary electrode (8) is positioned between the reference electrode (7) and the insulating partition plate (14), and the auxiliary electrode (8) is communicated with the corresponding signal input sheet (17) through a circuit line (9); one end of the detection tentacle (12) is positioned inside the electric signal box (13), and the other end of the detection tentacle (12) penetrates through the side wall of the electric signal box (13) and is connected to the outside of the electric signal box (13); the end part of the detection tentacle (12) is communicated with the end part of the injection port (18) through a shunt pipe (19); the end part of the detection tentacle (12) positioned in the electric signal box (13) is respectively connected with the corresponding signal input sheet (17) through a circuit line (9); the end part of the detection tentacle (12) is coated with a reaction layer (6), and the middle part of the detection tentacle (12) is designed to be hollow; the reaction layer (6) is a composite formed by carbon nano materials.

2. A microbial sensor according to claim 1, wherein: the cross section of the detection tentacle (12) is circular, the outer diameter range of the cross section of the detection tentacle (12) is 0.5mm-2mm, and the detection tentacle (12) has certain flexibility; the interior of the detection tentacle (12) can be made of silver or silver alloy materials.

3. A microbial sensor according to claim 2, wherein: the tail end of the detection tentacle (12) is fixedly connected with a hollow round ball (20), and small holes are uniformly distributed in the hollow round ball (20).

4. A microbial sensor according to claim 1, wherein: the cross section of the detection tentacle (12) is circular, the outer diameter range of the cross section of the detection tentacle (12) is 4-6 mm, the detection tentacle (12) is made of copper alloy or aluminum alloy, and a reaction layer (6) is sprayed on the outer part of the detection tentacle; the end part of the detection tentacle (12) is provided with a tip, and small holes are uniformly formed in the pipe wall of the detection tentacle (12).

5. A microbial sensor according to claim 1, wherein: the cross section of the detection tentacle (12) is rectangular, the length range of the rectangular cross section is 20mm-50mm, the width range of the rectangular cross section is 2mm-10mm, the detection tentacle (12) is made of copper alloy or aluminum alloy, a reaction layer (6) is sprayed on one side of the detection tentacle (12), and small holes are uniformly formed in the side face of the side.

6. A microbial sensor according to claim 1, wherein: a piston rod (21) is arranged in the injection syringe (10), and the connection part of the piston rod (21) and the end part of the syringe is connected through threads; the top end of the piston rod (21) is fixedly connected with an extrusion knob (22).

Technical Field

The invention belongs to the technical field of microbial detection, and particularly relates to a microbial sensor.

Background

With the development of biotechnology, various technical applications of microorganisms gradually enter the development of the day and night, wherein the microbial sensor is gradually developed, because the detection speed of the microbial sensor is high, the detection result can be obtained within minutes or tens of minutes, compared with the traditional detection method, the detection method needs a plurality of days of quick test time, and in addition, the detection cost of the microbial sensor is lower, so that the attention is paid more and more, and the development of the microbial sensor is also faster and more.

However, the corresponding microbial sensor has a corresponding short plate, firstly the microbial sensor needs living microbes or active specific enzymes or active proteins to detect target components, and utilizes the interaction between the biological activity and the specific components to detect the monitoring of signals such as fine electrorheological change or photochemical change in the reaction process of the microbial sensor and the specific components so as to obtain the specific situation of the target components, so that the maintenance of the activity of the microbes or microbe-derived products in the microbial sensor is very important in the storage process, and the special requirements on temperature, humidity, nutrients, oxygen and the like in the storage process are strict, besides, the microbes or microbe-derived products have specificity to the reaction of the target products, for example, a specific microbe only has a specific reaction to a specific chemical component, thereby enabling detection of the specific chemical component.

Disclosure of Invention

In order to make up for the defects of the prior art and solve the problems, the invention provides the microbial sensor, the injection needle cylinder is arranged to independently store the microorganisms or microbial derivatives for detection, and the microorganisms or microbial derivatives are independently injected into the microbial sensor for use during use, so that the used microorganisms or microbial derivatives can be independently stored, the storage difficulty is reduced, and meanwhile, different microorganisms or microbial derivatives can be used for detection, so that the microbial sensor is not a disposable consumable product any more.

The technical scheme adopted by the invention for solving the technical problems is as follows: the invention relates to a microbial sensor which comprises a shell, a signal identification element, a signal receiving sheet, a conductive substrate, an insulating substrate, a reaction layer, a reference electrode, an auxiliary electrode and a circuit wire, wherein the signal identification element is arranged on the shell; the device also comprises an injection needle cylinder, a needle tube fixing groove, a detection tentacle, an electric signal box, an insulating clapboard, a detection socket, a signal plug, a signal input sheet, an injection port and a shunt tube; a signal identification element is fixedly connected inside the shell; one end of the signal identification element is provided with a signal receiving sheet; a needle tube fixing groove is formed in the middle of the shell; the injection syringe is in sliding connection with the needle tube fixing groove and is positioned inside the needle tube fixing groove; a detection socket is arranged at one end of the shell, when the injection syringe slides to one end of the detection socket in the needle tube fixing groove, the needle head part of the injection syringe is positioned in the detection socket, and the signal receiving sheets are positioned in the detection socket and are positioned below the needle head part of the injection syringe; the end part of the signal plug is arranged corresponding to the detection socket; an injection port is arranged in the signal plug, and when the signal plug is positioned in the detection socket, the injection port is sleeved outside the needle head of the injection needle cylinder; the signal plug is internally provided with a signal input sheet which is positioned below the injection port, and when the signal plug is positioned in the detection socket, the signal input sheets are respectively sleeved outside the corresponding signal receiving sheets; the other end of the signal plug is fixedly connected to the side surface of the electrical signal box; the middle part of the electric signal box is fixedly connected with an insulating partition plate; one side of the electric signal box, which is close to the signal plug, is filled with an insulating substrate, and the other side of the electric signal box is filled with a conductive substrate; the insulating matrix is an organic polymer material; a reference electrode is arranged in the conductive substrate and is communicated with the corresponding signal input sheet through a circuit wire; an auxiliary electrode is arranged in the conductive substrate and is positioned between the reference electrode and the insulating partition plate, and the auxiliary electrode is communicated with the corresponding signal input sheet through a circuit wire; one end of the detection tentacle is positioned inside the electric signal box, and the other end of the detection tentacle penetrates through the side wall of the electric signal box and is connected to the outside of the electric signal box; the end part of the detection tentacle is communicated with the end part of the injection port through a shunt pipe; the end parts of the detection tentacles positioned in the electric signal box are respectively connected with the corresponding signal input sheets through circuit lines; the end part of the detection tentacle is coated with a reaction layer, and the middle part of the detection tentacle is designed to be hollow; the reaction layer is a composite formed by carbon nano materials. In the work, with the development of biotechnology, the technical application of microorganisms gradually enters the development of the change day and month, wherein the microbial sensor is gradually developed, because the microbial sensor has high detection speed, the detection result can be obtained within minutes or tens of minutes, compared with the traditional detection method, the microbial sensor needs a plurality of days of quick and quick detection time, and the detection cost of the microbial sensor is lower, so that the development of the microbial sensor is more and more emphasized, but the corresponding microbial sensor also has a corresponding short plate, firstly the microbial sensor needs living microorganisms or characteristic enzymes with activity or proteins with activity to detect target components, and the interaction between special components is utilized to detect the monitoring of signals such as fine electrorheological change or photochemical change in the reaction process of the two components, therefore, the specific situation of the target component is obtained, so that in the storage process, the activity of the microorganism or microorganism-derived products in the microorganism sensor is extremely important, the special requirements on temperature, humidity, nutrients, oxygen and the like in the storage process are severe, besides, the reaction of the microorganism or microorganism-derived products to the target product is mostly specific, for example, the special microorganism only has special reaction on a certain special chemical component, so that the special chemical component can be detected, the invention separately stores the microorganism or microorganism-derived products for detection by arranging the injection syringe, and separately injects the microorganism or microorganism-derived products into the microorganism sensor for use when in use, so that the used microorganism or microorganism-derived products can be separately stored, the storage difficulty is reduced, and simultaneously, different microorganisms or microorganism-derived products can be used for detection, when in use, the microorganism or microorganism derivatives are sucked into the injection syringe, and corresponding buffer solution (generally selected phosphate buffer solution) can be sucked to increase the surface liquid tension of the solution, so that larger and more stable detection liquid drops are formed, the detection environment is larger and more stable, the injection syringe is placed into the needle tube fixing groove, a signal plug is inserted, the injection syringe is slid, the needle head part of the injection syringe is butted with the injection port, at the moment, the microorganism or the mixture of the microorganism derivatives and the buffer solution is injected into the injection port, the mixture flows to each detection tentacle through the injection port and under the action of a shunt pipe, finally flows out through the detection tentacle, so that a liquid drop is formed between the detection tentacle and the object to be detected, and the inside of the liquid drop contains the microorganism or microorganism derivatives, when the organic polymer material is contacted with an object to be detected, the microorganisms or microorganism derivatives can react with corresponding chemical components on the surface of the object to be detected, the reaction degree is accelerated under the catalysis of a reaction layer, electric signals obtained by the reaction can be transmitted to the interior of an electric signal box through metal in a detection tentacle and can be subjected to current reaction with a reference electrode and an auxiliary electrode, current signals are amplified and transmitted to respective signal input sheets through respective circuit wires, and then transmitted to a signal identification element through a signal receiving sheet for detection and analysis, and finally, a result is obtained, the reaction layer adopts a carbon nano material compound, on one hand, the organic polymer material can well play an insulation effect, on the other hand, the organic polymer material can be finally cooled and solidified in a liquid mode, so that the internal circuit wires are shaped, and the production of the electric signal box is more convenient, also make the inside circuit line of signal of telecommunication box safer, in this process, because detect for the point contact between tentacle and the determinand, so when facing the determinand of surperficial unevenness, it also can be fine to detect the tentacle and accomplish the detection task, when needs detect another kind of determinand, after only needing to use the injection cylinder that is equipped with the washing liquid to wash signal of telecommunication box and detection tentacle, change other microorganism or microorganism derivatives, it can to detect new determinand, microorganism or microorganism derivatives can be stored alone, directly during the use with the injection cylinder extraction can, high durability and convenient use, detect material cyclic utilization simultaneously and also avoided extravagant.

Preferably, the cross section of the detection tentacle is circular, the outer diameter range of the cross section of the detection tentacle is 0.5mm-2mm, and the detection tentacle has certain flexibility; the interior of the detection tentacle can be made of silver or silver alloy materials. When the device works, the detection tentacles contact with the surface of the object to be detected to detect in the face of a conventional object to be detected, the soft detection tentacles are designed, soft silver or silver alloy is selected in the detection tentacles, the silver or silver alloy is good in ductility, so that the manufacturing difficulty of the detection tentacles can be reduced, the detection tentacles can be bent randomly before detection, the detection falling point of each detection tentacle is controlled to detect, and the detection range is enlarged.

Preferably, the tail end of the detection tentacle is fixedly connected with a hollow round ball, and small holes are uniformly distributed on the hollow round ball. During operation, hollow ball contacts with the determinand when detecting, under the effect of liquid surface tension, when the inside mixed liquid drop of efflux microorganism or microorganism derivative and buffer solution of hollow ball, hollow ball can form bigger contact surface with this liquid drop to increase the size of this liquid drop, make the detection environment that mixed liquid drop formed bigger more stable, thereby make the testing result also more accurate.

Preferably, the cross section of the detection tentacle is circular, the outer diameter range of the cross section of the detection tentacle is 4mm-6mm, the detection tentacle is made of copper alloy or aluminum alloy, and a reaction layer is sprayed on the outer part of the detection tentacle; the end part of the detection tentacle is provided with a tip, and small holes are uniformly formed in the wall of the detection tentacle. When the detection tentacle is used, the detection tentacle is inserted into the inside of the object to be detected, and the mixed solution of the microorganism or the microorganism derivative and the buffer solution flows out of the small hole in the tube wall of the detection tentacle and contacts with the object to be detected to form a detection environment for detection.

Preferably, the cross section of the detection tentacle is rectangular, the length range of the rectangular cross section is 20mm-50mm, the width range of the rectangular cross section is 2mm-10mm, the detection tentacle is made of copper alloy or aluminum alloy, a reaction layer is sprayed on one side of the detection tentacle, and small holes are uniformly formed in the side face of the side. When the detection tentacle is used, the detection tentacle is in spherical contact or in needle-tube-shaped cylindrical contact, which causes inconvenience for an object to be detected with a very flat surface and hard texture, so that the third detection tentacle for the object to be detected is provided, the detection tentacle is designed to be flat, and the detection tentacle is more favorable for being tightly combined with the object to be detected, so that the detection precision of the object to be detected is improved.

Preferably, a piston rod is arranged in the injection syringe, and the joint of the piston rod and the end of the syringe is in meshed connection through threads; and the top end of the piston rod is fixedly connected with an extrusion knob. When the injection syringe works, after a detection tentacle is placed, the injection syringe is extruded, and when a mixed liquid of microorganisms or microorganism derivatives and a buffer liquid is injected, large pressure is inevitably applied to the injection syringe, and the handheld shell can slightly shake.

The invention has the following beneficial effects:

1. according to the microbial sensor, the injection needle cylinder is arranged to store the microorganisms or microbial derivatives for detection independently, and the microorganisms or microbial derivatives are injected into the microbial sensor for use independently during use, so that the used microorganisms or microbial derivatives can be stored independently, the storage difficulty is reduced, meanwhile, different microorganisms or microbial derivatives can be used for detection, and the microbial sensor is not a disposable consumable.

2. According to the microbial sensor, the detection tentacles are arranged, so that the detection range is larger, detection can be performed on the uneven detection surface of some objects to be detected, and the phenomenon that the detection tentacles cannot be attached to the surface of the objects to be detected and cannot be detected is avoided.

Drawings

The invention will be further explained with reference to the drawings.

FIG. 1 is a perspective view of a housing and syringe barrel of the present invention;

FIG. 2 is a perspective view of the electrical signal box and a first type of detection tentacle of the present invention;

FIG. 3 is a front view of the electrical signal box;

FIG. 4 is a front cross-sectional view of the electrical signal box;

FIG. 5 is a cross-sectional view taken at A-A of FIG. 3;

FIG. 6 is a cross-sectional view of a first detection tentacle and hollow sphere of the present invention;

FIG. 7 is a cross-sectional view of a second version of a needle-like test tentacle of the present invention;

FIG. 8 is a cross-sectional view of an elongated test tentacle according to a third aspect of the test tentacle of the present invention;

in the figure: the device comprises a shell 1, a signal recognition element 2, a signal receiving sheet 3, a conductive substrate 4, an insulating substrate 5, a reaction layer 6, a reference electrode 7, an auxiliary electrode 8, a circuit line 9, an injection syringe 10, a needle tube fixing groove 11, a detection tentacle 12, an electric signal box 13, an insulating partition plate 14, a detection socket 15, a signal plug 16, a signal input sheet 17, an injection port 18, a shunt tube 19, a hollow ball 20, a piston rod 21 and an extrusion knob 22.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.

As shown in fig. 1 to 8, a microbial sensor according to the present invention includes a case 1, a signal recognition element 2, a signal receiving sheet 3, an electrically conductive substrate 4, an insulating substrate 5, a reaction layer 6, a reference electrode 7, an auxiliary electrode 8, and a circuit line 9; the device also comprises an injection syringe 10, a needle tube fixing groove 11, a detection tentacle 12, an electric signal box 13, an insulating clapboard 14, a detection socket 15, a signal plug 16, a signal input sheet 17, an injection port 18 and a shunt tube 19; a signal identification element 2 is fixedly connected inside the shell 1; a signal receiving sheet 3 is arranged at one end of the signal identification element 2; a needle tube fixing groove 11 is formed in the middle of the shell 1; the injection syringe 10 is in sliding connection with the needle tube fixing groove 11, and the injection syringe 10 is positioned inside the needle tube fixing groove 11; one end of the shell 1 is provided with a detection socket 15, when the injection syringe 10 slides to one end of the detection socket 15 in the needle tube fixing groove 11, the needle head part of the injection syringe 10 is positioned in the detection socket 15, and the signal receiving sheets 3 are positioned in the detection socket 15 and below the needle head part of the injection syringe 10; the end part of the signal plug 16 is arranged corresponding to the detection jack 15; an injection port 18 is arranged inside the signal plug 16, and when the signal plug 16 is positioned inside the detection socket 15, the injection port 18 is sleeved outside the needle head of the injection syringe 10; the signal plug 16 is internally provided with a signal input sheet 17, the signal input sheet 17 is positioned below the injection port 18, and when the signal plug 16 is positioned in the detection socket 15, the signal input sheets 17 are respectively sleeved outside the corresponding signal receiving sheets 3; the other end of the signal plug 16 is fixedly connected to the side surface of the electric signal box 13; an insulating partition plate 14 is fixedly connected to the middle part of the electric signal box 13; one side of the electric signal box 13, which is close to the signal plug 16, is filled with an insulating matrix 5, and the other side of the electric signal box 13 is filled with a conductive matrix 4; the insulating matrix 5 is an organic polymer material; a reference electrode 7 is arranged in the conductive substrate 4, and the reference electrode 7 is communicated with a corresponding signal input sheet 17 through a circuit line 9; an auxiliary electrode 8 is arranged in the conductive substrate 4, the auxiliary electrode 8 is positioned between the reference electrode 7 and the insulating partition 14, and the auxiliary electrode 8 is communicated with a corresponding signal input sheet 17 through a circuit line 9; one end of the detection tentacle 12 is positioned inside the electric signal box 13, and the other end of the detection tentacle 12 penetrates through the side wall of the electric signal box 13 and is connected to the outside of the electric signal box 13; the end part of the detection tentacle 12 is communicated with the end part of the injection port 18 through a shunt tube 19; the end parts of the detection tentacles 12 positioned in the electric signal box 13 are respectively connected with corresponding signal input sheets 17 through circuit lines 9; the end part of the detection tentacle 12 is coated with a reaction layer 6, and the middle part of the detection tentacle 12 is designed to be hollow; the reaction layer 6 is a composite formed by carbon nano materials. In the work, with the development of biotechnology, the technical application of microorganisms gradually enters the development of the change day and month, wherein the microbial sensor is gradually developed, because the microbial sensor has high detection speed, the detection result can be obtained within minutes or tens of minutes, compared with the traditional detection method, the microbial sensor needs a plurality of days of quick and quick detection time, and the detection cost of the microbial sensor is lower, so that the development of the microbial sensor is more and more emphasized, but the corresponding microbial sensor also has a corresponding short plate, firstly the microbial sensor needs living microorganisms or characteristic enzymes with activity or proteins with activity to detect target components, and the interaction between special components is utilized to detect the monitoring of signals such as fine electrorheological change or photochemical change in the reaction process of the two components, therefore, the specific situation of the target component is obtained, so that in the storage process, the activity of the microorganism or microorganism-derived products in the microorganism sensor is extremely important, the special requirements on temperature, humidity, nutrients, oxygen and the like in the storage process are severe, besides, the reaction of the microorganism or microorganism-derived products to the target product is mostly specific, for example, the special microorganism only has special reaction on a certain special chemical component, so that the special chemical component can be detected, the invention separately stores the microorganism or microorganism-derived products for detection by arranging the injection syringe 10, and separately injects the microorganism or microorganism-derived products into the microorganism sensor for use when in use, so that the used microorganism or microorganism-derived products can be separately stored, the storage difficulty is reduced, and simultaneously, different microorganisms or microorganism-derived products can be used for detection, when the microorganism sensor is used, microorganisms or microorganism derivatives are sucked into the injection needle cylinder 10, corresponding buffer solution (generally selected phosphate buffer solution) can be sucked to increase the surface liquid tension of the solution, so that larger and more stable detection liquid drops are formed, the detection environment is larger and more stable, the injection needle cylinder 10 is placed into the needle tube fixing groove 11, the signal plug 16 is inserted, the injection needle cylinder 10 is slid, the needle head part of the injection needle cylinder 10 is completely butted with the injection port 18, at the moment, the microorganisms or the mixed liquid of the microorganism derivatives and the buffer solution is injected into the injection port 18, the mixed liquid respectively flows to each detection tentacle 12 through the injection port 18 under the action of the shunt tube 19, and finally flows out through the interior of the detection tentacle 12, so that a liquid drop is formed between the detection tentacle 12 and an object to be detected, the liquid drop contains microorganisms or microorganism derivatives, when the liquid drop is contacted with an object to be detected, the microorganisms or the microorganism derivatives can react with corresponding chemical components on the surface of the object to be detected, the reaction degree is accelerated under the catalysis of the reaction layer 6, electric signals obtained by the reaction can be transmitted to the interior of the electric signal box 13 through metal in the detection tentacle 12 and can be subjected to current reaction with the reference electrode 7 and the auxiliary electrode 8, current signals are amplified and transmitted to the respective signal input sheet 17 through the respective circuit wire 9, and then the electric signals are transmitted to the signal identification element 2 through the signal receiving sheet 3 for detection and analysis, and finally, a result is obtained, the reaction layer 6 adopts a carbon nano material compound, on one hand, the organic high polymer material can well play an insulation effect, on the other hand, the organic high polymer material can be finally cooled and solidified in a liquid mode, make internal circuit line 9 set shape, when producing electric signal box 13, it is more convenient, also make electric signal box 13 internal circuit line 9 safer, in this process, because detect for the point contact between tentacle 12 and the determinand, so when facing the determinand of surperficial unevenness, it also can be fine to detect tentacle 12 completion detection task, when needs detect another kind of determinand, only need use the injection cylinder 10 that is equipped with the washing liquid to wash after electric signal box 13 and detection tentacle 12, change other microorganism or microorganism derivatives, to new determinand detect can, microorganism or microorganism derivatives can be stored alone, during the use directly with injection cylinder 10 extraction can, high durability and convenient use, detect material cyclic utilization simultaneously and also avoided the waste.

As an embodiment of the present invention, the cross section of the detection tentacle 12 is circular, the outer diameter of the cross section of the detection tentacle 12 ranges from 0.5mm to 2mm, and the detection tentacle 12 has a certain flexibility; the interior of the detection tentacle 12 can be made of silver or silver alloy material. In the working process, in the face of a conventional object to be detected, the detection tentacle 12 is in contact with the surface of the object to be detected for detection, the soft detection tentacle 12 is designed, soft silver or silver alloy is selected in the detection tentacle 12, and the silver or silver alloy has good ductility, so that the manufacturing difficulty of the detection tentacle 12 can be reduced, the detection tentacle 12 can be bent randomly before detection, the detection falling point of each detection tentacle 12 is controlled for detection, and the detection range is enlarged.

As an embodiment of the present invention, the end of the detection tentacle 12 is fixedly connected with a hollow sphere 20, and small holes are uniformly distributed on the hollow sphere 20. During operation, hollow ball 20 contacts with the determinand when detecting, under the effect of liquid surface tension, when the inside mixed liquid drop of flowing out microorganism or microorganism derivative and buffer solution of hollow ball 20, hollow ball 20 can form bigger contact surface with this liquid drop to increase the size of this liquid drop, make the detection environment that mixed liquid drop formed bigger more stable, thereby make the testing result also more accurate.

As an embodiment of the invention, the cross section of the detection tentacle 12 is circular, the outer diameter range of the cross section of the detection tentacle 12 is 4mm-6mm, the detection tentacle 12 is made of copper alloy or aluminum alloy, and the reaction layer 6 is sprayed on the outer part; the end part of the detection tentacle 12 is provided with a tip, and small holes are uniformly formed in the pipe wall of the detection tentacle 12. During operation, the interior of the object to be detected needs to be detected under the specific condition without cutting the object to be detected, so the invention provides a second different detection tentacle 12, the detection tentacle 12 is designed into a needle tube shape, a copper alloy or an aluminum alloy with higher rigidity is used, the detection tentacle 12 is inserted into the object to be detected for detection during use, and mixed liquid of microorganisms or microorganism derivatives and buffer liquid flows out of small holes in the tube wall of the detection tentacle 12 and contacts with the object to be detected to form a detection environment for detection.

As an embodiment of the invention, the cross section of the detection tentacle 12 is rectangular, the length range of the rectangular cross section is 20mm-50mm, the width range of the rectangular cross section is 2mm-10mm, the detection tentacle 12 is made of copper alloy or aluminum alloy, one side of the detection tentacle 12 is sprayed with the reaction layer 6, and the side surface of the side is uniformly provided with small holes. During operation, for an object to be detected with a very flat surface and a hard texture, the detection tentacle 12 is in spherical contact or in a needle-tube-shaped cylindrical contact, which causes inconvenience, so the invention provides a third detection tentacle 12 for the object to be detected, and the detection tentacle 12 is designed to be in a flat plate shape, which is more beneficial to the tight combination between the detection tentacle 12 and the object to be detected, thereby increasing the detection precision of the object to be detected.

As an embodiment of the invention, a piston rod 21 is arranged inside the injection syringe 10, and the connection part of the piston rod 21 and the end part of the syringe is connected through threads; the top end of the piston rod 21 is fixedly connected with an extrusion knob 22. When the injection syringe is in work, after the detection tentacle 12 is placed, the injection syringe 10 is extruded, and when a mixed liquid of microorganisms or microorganism derivatives and a buffer solution is injected, large pressure is inevitably applied to the injection syringe 10, and the shell 1 is held by a hand, so that the shell 1 may slightly shake.

In the work, with the development of biotechnology, the technical application of microorganisms gradually enters the development of the change day and month, wherein the microbial sensor is gradually developed, because the microbial sensor has high detection speed, the detection result can be obtained within minutes or tens of minutes, compared with the traditional detection method, the microbial sensor needs a plurality of days of quick and quick detection time, and the detection cost of the microbial sensor is lower, so that the development of the microbial sensor is more and more emphasized, but the corresponding microbial sensor also has a corresponding short plate, firstly the microbial sensor needs living microorganisms or characteristic enzymes with activity or proteins with activity to detect target components, and the interaction between special components is utilized to detect the monitoring of signals such as fine electrorheological change or photochemical change in the reaction process of the two components, therefore, the specific situation of the target component is obtained, so that in the storage process, the activity of the microorganism or microorganism-derived products in the microorganism sensor is extremely important, the special requirements on temperature, humidity, nutrients, oxygen and the like in the storage process are severe, besides, the reaction of the microorganism or microorganism-derived products to the target product is mostly specific, for example, the special microorganism only has special reaction on a certain special chemical component, so that the special chemical component can be detected, the invention separately stores the microorganism or microorganism-derived products for detection by arranging the injection syringe 10, and separately injects the microorganism or microorganism-derived products into the microorganism sensor for use when in use, so that the used microorganism or microorganism-derived products can be separately stored, the storage difficulty is reduced, and simultaneously, different microorganisms or microorganism-derived products can be used for detection, when the microorganism sensor is used, microorganisms or microorganism derivatives are sucked into the injection needle cylinder 10, corresponding buffer solution (generally selected phosphate buffer solution) can be sucked to increase the surface liquid tension of the solution, so that larger and more stable detection liquid drops are formed, the detection environment is larger and more stable, the injection needle cylinder 10 is placed into the needle tube fixing groove 11, the signal plug 16 is inserted, the injection needle cylinder 10 is slid, the needle head part of the injection needle cylinder 10 is completely butted with the injection port 18, at the moment, the microorganisms or the mixed liquid of the microorganism derivatives and the buffer solution is injected into the injection port 18, the mixed liquid respectively flows to each detection tentacle 12 through the injection port 18 under the action of the shunt tube 19, and finally flows out through the interior of the detection tentacle 12, so that a liquid drop is formed between the detection tentacle 12 and an object to be detected, the liquid drop contains microorganisms or microorganism derivatives, when the liquid drop is contacted with an object to be detected, the microorganisms or the microorganism derivatives can react with corresponding chemical components on the surface of the object to be detected, the reaction degree is accelerated under the catalysis of the reaction layer 6, electric signals obtained by the reaction can be transmitted to the interior of the electric signal box 13 through metal in the detection tentacles 12 and can generate current reaction with the reference electrode 7 and the auxiliary electrode 8, current signals are amplified and transmitted to the respective signal input sheet 17 through the respective circuit lines 9, and then the signals are transmitted to the signal identification element 2 through the signal receiving sheet 3 for detection and analysis, and finally, a result is obtained, during the process, the interior of the detection tentacles 12 is made of soft silver or silver alloy and can be bent randomly before detection, the detection falling point of each detection tentacle 12 is selected for detection, and the detection range is enlarged, and facing the object to be detected with uneven surface, the detection tentacle 12 can well complete the detection task, when another object to be detected needs to be detected, only the injection syringe 10 filled with the cleaning solution needs to be used for cleaning the electric signal box 13 and the detection tentacle 12, other microorganisms or microorganism derivatives are replaced, and the new object to be detected is detected, the microorganisms or microorganism derivatives can be stored independently, and can be directly extracted by the injection syringe 10 during use, so that the use is convenient, and meanwhile, the detection material is recycled, and the waste is avoided.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.