Infant formula goat milk powder added with liposome simulating breast milk fat structure and preparation method thereof

文档序号：1581750 发布日期：2020-02-04 浏览：35次中文

阅读说明：本技术 一种添加模拟母乳脂肪结构的脂质体的婴幼儿配方羊奶粉及其制备方法 (Infant formula goat milk powder added with liposome simulating breast milk fat structure and preparation method thereof ) 是由李晓东张宇刘璐田松凡林爽潘悦于 2019-11-14 设计创作，主要内容包括：本发明公开了一种添加模拟母乳脂肪结构的脂质体(富含脂肪球膜及结构脂OPO)的婴幼儿配方羊奶粉及其制备方法。本发明提供的婴儿配方奶粉中MFGM蛋白含量0.16％-0.64％,MFGM磷脂含量0.14％-0.56％,结构油脂OPO含量3％-10.4％,本发明以脱脂羊乳为原料,除普通营养成分,还添加了模拟母乳脂肪结构的脂质体。本配方奶粉中油脂经3种植物油及OPO混合配比组成,并通过高压微射流均质技术将复配油包用MFGM包裹起来添加到脱脂羊奶中,制成婴幼儿配方羊奶粉。本发明以母乳为参照对象,添加乳脂肪球膜以及OPO到配方粉内,使其营养成分尤其是脂肪球组成和结构更接近母乳,促进新生儿大脑和神经发育,提高认知和抗感染能力,有效降低传统配方奶粉中棕榈酸引起的钙皂便秘问题,易于婴幼儿消化吸收。(The invention discloses infant formula goat milk powder added with liposome (rich in fat globule membrane and structural lipid OPO) simulating breast milk fat structure and a preparation method thereof. The infant formula milk powder provided by the invention contains 0.16-0.64% of MFGM protein, 0.14-0.56% of MFGM phospholipid and 3-10.4% of structure oil OPO, and is prepared by taking skim goat milk as a raw material and adding liposome simulating the fat structure of breast milk besides common nutrient components. The formula milk powder is prepared by mixing and proportioning 3 vegetable oils and OPO, wrapping a compound oil pocket by MFGM through a high-pressure micro-jet homogenization technology, and adding the wrapped oil pocket into skimmed goat milk to prepare the infant formula goat milk powder. According to the invention, the breast milk is taken as a reference object, and the milk fat globule membrane and the OPO are added into the formula powder, so that the nutritional ingredients, especially the fat globule composition and structure are closer to the breast milk, the brain and nerve development of a newborn is promoted, the cognition and anti-infection capability is improved, the calcium soap constipation problem caused by palmitic acid in the traditional formula milk powder is effectively reduced, and the milk fat globule is easy to digest and absorb by infants.)

1. A compound grease simulating breast milk fatty acid is characterized in that: the compound grease is prepared by mixing the following components in percentage by weight: 28-37% of sheep milk fat, 30-40% of OPO, 19-25% of palm oil, 0-6.06% of sunflower seed oil and 8-16.95% of soybean oil.

2. The preparation method of the liposome simulating the fat structure of breast milk is characterized by comprising the following steps:

1) dissolving a certain amount of MFGM concentrated whey protein in different mass percentages (1%, 2%, 3%, 4%) in Milli-Q water, and stirring in a water bath with magnetic force (600-;

2) adjusting the pH value of the polar lipid solution to 6.5-6.8 by using 0.5mol/L and 5mol/L sodium hydroxide solution;

3) then adding the compounded vegetable oil pocket into the MFGM concentrated whey protein solution (the ratio of the oil pocket to the protein solution is 1: 9-3: 7) all samples were processed with a shear at 6000-;

4) then using high-pressure microjet homogenizer to make cyclic treatment under 5-10Mpa for 2-4 times to homogenize the emulsion.

3. An infant formula goat milk powder containing the breast milk fat structure mimicking liposome of claim 2, comprising the following components: according to the weight portion, 400 portions of defatted goat milk powder, 450 portions of desalted goat whey powder, 340 portions of liposome, 1.4 to 2.5 portions of docosahexaenoic acid, 2.4 to 3.2 portions of arachidonic acid, 10 to 30 portions of fructo-oligosaccharide, 1 to 30 portions of galacto-oligosaccharide, 1 to 3.0 portions of vitamin premix, 1.5 to 2.5 portions of mineral premix, 2.5 to 3.5 portions of calcium carbonate, 0.6 to 3.6 portions of choline, 0.1 to 0.25 portion of L-carnitine, 0.4 to 0.5 portion of nucleotide and 0.02 to 0.1 portion of lutein.

4. The infant formula goat milk powder containing the liposome simulating breast milk fat structure as claimed in claim 3, wherein the vitamin premix is prepared by mixing 45% -55% of ascorbic acid, 5% -10% of dl- α -tocopheryl acetate, 1% -5% of nicotinamide, 0.3% -1% of retinyl acetate, 0.8% -2% of D-calcium pantothenate, 0.1% -1% of thiamine hydrochloride, 0.06% -0.6% of pyridoxine hydrochloride, 0.03% -0.25% of folic acid, 0.03% -0.1% of plant menadione, 0.0085% -0.05% of D-biotin, 0.0008% -0.0012% of cholecalciferol, and 0.0006% -0.0056% of cyanobacteria.

5. The infant formula goat milk powder containing liposomes simulating the fatty structure of breast milk according to claim 3, wherein: the mineral premix is prepared by mixing the following components in percentage by weight: 30 to 50 percent of calcium carbonate, 17 to 20 percent of calcium chloride, 12 to 20 percent of sodium citrate, 15 to 30 percent of potassium citrate, 5 to 15 percent of magnesium chloride, 1 to 3 percent of ferrous sulfate, 0.44 to 0.98 percent of zinc sulfate, 0.05 to 0.75 percent of copper sulfate, 0.01 to 0.06 percent of manganese sulfate, 0.02 to 0.048 percent of sodium selenite and 0.010 to 0.020 percent of potassium iodide.

6. A method of preparing an infant formula goat milk powder containing liposomes mimicking the adipose structure of human milk of claim 3, comprising the steps of:

the method comprises the following steps: first-stage mixing, namely mixing the degreased goat milk powder, the desalted goat whey powder, the mineral substances and the vitamins in proportion, adding the mixture into preheated purified water, and uniformly mixing to obtain mixed feed liquid A;

step two: a second-stage mixing step, in which the material liquid A and liposome (a mixed oil bag coated by a milk fat spherical membrane) are preheated and mixed to obtain mixed material liquid B;

step three: shearing and dispersing, namely dispersing the material liquid B for 5-10min at 7000-12000r/min by adopting a high-speed dispersion machine;

step four: homogenizing, namely homogenizing the mixed solution prepared in the step three in a homogenizer to obtain a mixed solution;

step five: sterilizing, namely pre-sterilizing the mixed solution after homogenizing in the step four, wherein the sterilization temperature is 110-;

step six: concentrating, namely sterilizing and concentrating the mixed solution obtained after the sterilization in the step five in an evaporator to obtain concentrated mixed solution;

step seven: spray drying, namely feeding the mixed solution concentrated in the step six into a spray drying tower for spray drying to obtain first mixed powder;

step eight: fluidized bed drying, namely performing secondary drying on the spray-dried first mixed powder by using a fluidized bed to obtain second mixed powder;

step nine: dry-mixing, namely premixing fructo-oligosaccharide, calcium carbonate, L-carnitine, nucleotide, lutein, docosahexaenoic acid and arachidonic acid in a ratio, and uniformly mixing the premixed material and second mixed powder in a dry mixer to obtain the infant formula goat milk powder;

and step ten, packaging, namely packaging by vacuum nitrogen charging.

7. The method for preparing infant formula goat milk powder containing liposomes simulating the fat structure of breast milk according to claim 6, wherein the method comprises the following steps: in the first step and the second step, the temperature of the preheating and mixing is 45-60 ℃.

8. The method for preparing infant formula goat milk powder containing liposomes simulating the fat structure of breast milk according to claim 6, wherein the method comprises the following steps: in the fourth step, the homogenizing temperature is 45-55 ℃ and the homogenizing pressure is 15-25 Mpa.

9. The method for preparing infant formula goat milk powder containing liposomes simulating the fat structure of breast milk according to claim 6, wherein the method comprises the following steps: in step six, the concentration temperature is: 55-70 ℃.

10. The method for preparing infant formula goat milk powder containing liposomes simulating the fat structure of breast milk according to claim 6, wherein the method comprises the following steps: in step seven, the spray drying conditions are: the spraying pressure is 14MPa, the air inlet temperature is 165-185 ℃, and the air exhaust temperature is 75-90 ℃.

Technical Field

The invention relates to the field of infant formula milk powder, in particular to infant formula goat milk powder added with liposome (rich in OPO structure ester and fat globule membrane) simulating breast milk fat structure and a preparation method thereof.

Background

Breast milk (HM) is the sole source of energy and bioactive components during infant growth and development and is considered the gold standard to support optimal growth and development of the newborn. The formula milk powder is used as a substitute and a supplement of breast milk and also needs to meet various nutrient substances required by the growth and development of infants. At present, the nutrition of breast milk is simulated by adding the nutrient types contained in the breast milk, for example, the types and the amounts of the nutrient types such as protein, fat, carbohydrate, mineral, vitamin and the like are as close as possible to the breast milk, so that the infant fed by the milk powder can grow healthily.

Cow milk and goat milk can be used as milk-based sources of infant formula, but the nutrient composition and content of the cow milk and the goat milk are obviously different, and the protein composition, the non-protein nitrogen content, the fatty acid composition and the oligosaccharide species of the goat milk are closer to those of human milk compared with cow milk and are used for developing infant formula goat milk powder. The molecular structure of the goat milk is small, is only one third of that of cow milk, the digestion rate of the goat milk is 5-6 times higher than that of cow milk, the goat milk is easier to digest and absorb, the goat milk is not fat, and the contents of trace elements and vitamins are far higher than that of cow milk.

In addition, although the fatty acid composition of goat milk is closer to that of human milk than cow milk, the content of medium-short chain fatty acid is up to 20%, and sufficient linoleic acid and arachidonic acid are contained, the fatty acid composition of goat milk is still different from that of human milk fat, and the fatty acid composition still needs to be adjusted by using compound grease. Research has shown that the highest content of saturated fatty acids in human milk is palmitic acid, which accounts for the most important energy-producing fatty acids of the human body, 17% -25% of mature human milk fatty acids, of which up to 70% of palmitic acid is esterified at the sn-2 position of the triglyceride, and 1, 3-dioleoyl-2-palmityl triglyceride (OPO), which is typically sn-2 palmitate. The OPO structure fat takes animal and vegetable oil as raw material, and the content of the palmitic acid at the sn-2 position is up to more than 40 percent under the condition of lipase catalysis fat exchange, thereby being close to the level of breast milk. The unique sn-2 palmitic acid structure of the OPO structure fat is not easy to form calcium soap during digestion, so that the constipation and the defecation difficulty of infants are not easy to cause, the digestion and absorption of fatty acid and calcium can be effectively improved, and bones are strengthened. The OPO structural lipid can be combined with soluble dietary fiber, increase the number of bifidobacteria, improve the intestinal flora balance of infants and children, and improve natural resistance.

The infant formula milk powder on the market only simulates the nutrient components of breast milk to the maximum extent, but the physical state of the infant formula milk powder cannot be restored, and the most important difference is the structure of fat globules. In current infant formulas, the particle size of the fat droplets is around 0.4um and protein is the main emulsifier for the fat droplets, which results in a stable and reproducible product with a long shelf life, but the structure of the fat droplets is very different from that of breast milk. The lipid in the infant formula milk powder mainly comes from various vegetable oils, so that the milk powder is lack of MFGM components, the lack of MFGM in the milk powder causes the difference between the milk powder and the breast milk phospholipid, and the milk powder lipid droplets lack the package of MFGM and exist in the form of large fat droplets and are not easy to digest and absorb by infants, so that the addition of MFGM can enable the nutritional function of the infant formula milk powder to be closer to breast milk. In recent years, the properties of MFGM components have been studied and many health benefits have been proposed, and these MFGM biological activities have led to their notion of potential as nutraceuticals in food applications. However, the potential of MFGM is not fully realized, and only a few infant milk powders are added with cow milk MFGM components, so the establishment of the composition and the three-dimensional structure of human milk MFGM will be a new trend for human milk research.

Disclosure of Invention

In view of the above, the present invention aims to prepare a liposome simulating the fatty acid composition and fat structure of breast milk, which can make the fatty acid composition and fat structure of formula goat milk powder closer to breast milk after being added to formula goat milk powder, and is rich in fat globule membrane and structural oil OPO.

In order to achieve the purpose, the invention adopts the following technical scheme:

the first aspect of the invention provides compound grease simulating breast milk fatty acid, which is prepared by mixing the following components in percentage by weight: 28-37% of sheep milk fat, 30-40% of OPO, 19-25% of palm oil, 0-6.06% of sunflower seed oil and 8-16.95% of soybean oil.

An infant formula goat milk powder containing liposomes simulating the fatty acid composition and fat structure of breast milk, comprising the following components: according to the weight portion, 400 portions of defatted goat milk powder, 450 portions of desalted goat whey powder, 340 portions of liposome, 1.4 to 2.5 portions of docosahexaenoic acid, 2.4 to 3.2 portions of arachidonic acid, 10 to 30 portions of fructo-oligosaccharide, 1 to 30 portions of galacto-oligosaccharide, 1 to 3.0 portions of vitamin premix, 1.5 to 2.5 portions of mineral premix, 2.5 to 3.5 portions of calcium carbonate, 0.6 to 3.6 portions of choline, 0.1 to 0.25 portion of L-carnitine, 0.4 to 0.5 portion of nucleotide and 0.02 to 0.1 portion of lutein.

Preferably, the vitamin premix is prepared by mixing 45-55% of ascorbic acid, 5-10% of dl- α -tocopheryl acetate, 1-5% of nicotinamide, 0.3-1% of retinyl acetate, 0.8-2% of D-calcium pantothenate, 0.1-1% of thiamine hydrochloride, 0.06-0.6% of pyridoxine hydrochloride, 0.03-0.25% of folic acid, 0.03-0.1% of plant menadione, 0.0085-0.05% of D-biotin, 0.0008-0.0012% of cholecalciferol and 0.0006-0.0056% of cyanamide according to weight percentage.

Preferably, the mineral premix is prepared by mixing the following components in percentage by weight: 30 to 50 percent of calcium carbonate, 17 to 20 percent of calcium chloride, 12 to 20 percent of sodium citrate, 15 to 30 percent of potassium citrate, 5 to 15 percent of magnesium chloride, 1 to 3 percent of ferrous sulfate, 0.44 to 0.98 percent of zinc sulfate, 0.05 to 0.75 percent of copper sulfate, 0.01 to 0.06 percent of manganese sulfate, 0.02 to 0.048 percent of sodium selenite and 0.010 to 0.020 percent of potassium iodide.

The invention relates to a preparation method of a liposome simulating breast milk fat globule structure, which comprises the following steps:

1) dissolving a certain amount of MFGM concentrated whey protein in different mass percentages (1%, 2%, 3%, 4%) in Milli-Q water, and stirring in a water bath with magnetic force (600-;

2) adjusting the pH value of the polar lipid solution to 6.5-6.8 by using 0.5mol/L and 5mol/L sodium hydroxide solution;

3) then adding the compounded vegetable oil pocket into the protein solution (the ratio of the oil pocket to the protein solution is 1: 9-3: 7). All samples were treated with a shear at 6000-;

4) then using high-pressure microjet homogenizer to make cyclic treatment under 5-10Mpa for 2-4 times to homogenize the emulsion.

Preferably, the mass percentage of the MFGM concentrated whey protein in the ultrapure water in step 1) is 4%;

preferably, the magnetic stirring conditions of the water bath in the step 1) are 800rmp and 45 ℃;

preferably, the ratio of oil to protein solution in step 3) is 2: 8;

preferably, the shearing time in the step 3) is 5 min;

preferably, the high-pressure homogenization in the step 4) is performed for 3 times, and the homogenization pressure is 10 Mpa;

the second aspect of the invention provides a preparation method of infant formula goat milk powder containing simulated breast milk fatty acid composition and fat structure, which specifically comprises the following steps:

the method comprises the following steps: mixing the first-stage materials, namely mixing the degreased goat milk powder, the desalted goat whey powder, the mineral substances and the vitamins according to a proportion, adding the mixture into purified water at the temperature of 45-60 ℃, and uniformly mixing to obtain mixed material liquid A;

step two: a second-stage mixing step, in which the material liquid A and a mixed oil bag coated by a milk fat spherical membrane are preheated and mixed to obtain mixed material liquid B;

step three: shearing and dispersing, namely dispersing the material liquid B for 5-10min at 7000-12000r/min by adopting a high-speed dispersion machine;

step four: homogenizing, namely homogenizing the mixed solution prepared in the step three in a homogenizer to obtain the mixed solution, wherein the homogenizing pressure is 15-25 Mpa;

step five: sterilizing, namely pre-sterilizing the mixed solution after homogenizing in the step four, wherein the sterilization temperature is 110-;

step six: and (4) concentrating, namely sterilizing and concentrating the mixed solution obtained after the sterilization of the product in the step five in an evaporator, wherein the concentration temperature is as follows: 55-70 ℃;

step seven: spray drying, namely feeding the mixed solution concentrated in the step six into a spray drying tower for spray drying to obtain first mixed powder;

step eight: fluidized bed drying, namely performing secondary drying on the spray-dried first mixed powder by using a fluidized bed to obtain second mixed powder;

and step ten, packaging, namely packaging by vacuum nitrogen charging.

Preferably, the concentration of the preheated mixture in the second step is 40-50 ℃;

preferably, the spray drying conditions in step seven are: the spraying pressure is 14MPa, the air inlet temperature is 165-185 ℃, and the air exhaust temperature is 75-90 ℃.

The invention has the advantages that:

through the technical scheme, compared with the prior art, the invention has the following beneficial effects:

based on the fatty acid composition and the fat structure of breast milk, the invention provides a high-quality specific compound grease simulating the fatty acid composition of breast milk for the invention concept of matching sheep milk fat with specific vegetable oil and structural grease OPO.

The invention adopts whey protein powder rich in milk fat globule membrane and mixed vegetable oil rich in structural fat separated from cow milk as raw materials, firstly embeds compound grease by using the fat globule membrane to obtain fat droplets with the particle size close to that of breast milk fat globules, and improves the content of bioactive substances (MFGM-protein, lactoferrin, phospholipid, ganglioside, sphingomyelin, phosphatidylcholine and the like) with special functional components and 1, 3-dioleate-2-palmitic acid glycerol triacetate in fat through formula adjustment, so that the content of the bioactive substances is closer to that of breast milk, the milk fat globule membrane and an OPO structure on the fatty acid composition and the fat structure, the health result difference between formula milk feeding and breast feeding babies is reduced, the absorption of fatty acid and calcium by the babies is promoted, the cognitive ability of the babies is improved, and the incidence rate of intestinal diseases is reduced, the formula has good effects on both nerve development and infection prevention, and is suitable for development of formula milk powder for premature infants and infants.

Detailed Description

The present invention will now be described more fully hereinafter with reference to specific embodiments, but it is to be understood that the embodiments described are merely exemplary of some, but not all, embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

The compound grease simulating breast milk fatty acid is prepared by mixing the following components in percentage by weight: sheep milk fat 40%, OPO 33%, palm oil 21%, sunflower seed oil 2%, and soybean oil 15%

An infant formula goat milk powder containing the compound grease simulating breast milk fatty acid comprises the following components: 300 parts of defatted goat milk powder, 420 parts of desalted goat whey powder, 236 parts of liposome, 2 parts of docosahexaenoic acid, 2.8 parts of arachidonic acid, 20 parts of fructo-oligosaccharide, 10 parts of galacto-oligosaccharide, 3 parts of vitamin premix, 2 parts of mineral premix, 2.8 parts of calcium carbonate, 2.3 parts of choline, 0.22 part of L-carnitine, 0.44 part of nucleotide and 0.08 part of lutein.

Preferably, the vitamin premix is prepared by mixing 50% of ascorbic acid, 6% of dl- α -tocopheryl acetate, 3.5% of nicotinamide, 1.3% of D-calcium pantothenate, 0.15% of thiamine hydrochloride, 0.3% of pyridoxine hydrochloride, 0.7% of retinyl acetate, 0.14% of folic acid, 0.06% of phytomenadione, 0.01% of biotin, 0.0009% of cholecalciferol, 0.01% of cyanocobalamin and 25% of inositol by weight.

Preferably, the mineral premix is prepared by mixing the following components in percentage by weight: 35.6% of calcium carbonate, 17.38% of calcium chloride, 17.6% of sodium citrate, 16.8% of potassium citrate, 10.2% of magnesium chloride, 1.6% of ferrous sulfate, 0.65% of zinc sulfate, 0.10% of copper sulfate, 0.025% of manganese sulfate, 0.03% of sodium selenite and 0.015% of potassium iodide.

A preparation method of a liposome simulating breast milk fat globule structure comprises the following steps:

1) dissolving a certain amount of MFGM concentrated whey protein in Milli-Q water at a mass percent of 2%, and magnetically stirring in a water bath (750rpm, 45 ℃) for 12 hours;

2) adjusting the pH value of the polar lipid solution to 6.7 by using 0.5mol/L and 5mol/L sodium hydroxide solution;

3) then adding the compounded vegetable oil pocket into the protein solution (the ratio of the oil pocket to the protein solution is 1: 9) all samples were processed with a shear at 6000rpm/min for 10 minutes;

4) then homogenizing the emulsion by using a high-pressure micro-jet homogenizer and circulating and treating for 3 times under 10 Mpa.

A preparation method of the infant formula goat milk powder containing the compound grease simulating breast milk fatty acid comprises the following steps:

the method comprises the following steps: mixing the first-stage materials, namely mixing the degreased goat milk powder, the desalted goat whey powder, the mineral substances and the vitamins according to a proportion, adding the mixture into purified water at 50 ℃, and uniformly mixing to obtain mixed material liquid A;

step two: a second-stage mixing step, in which the material liquid A and a mixed oil bag coated by a milk fat spherical membrane are preheated and mixed to obtain mixed material liquid B;

step three: shearing and dispersing, namely dispersing the feed liquid B for 8min at 7000r/min by adopting a high-speed dispersion machine;

step four: homogenizing, namely homogenizing the mixed solution prepared in the step three in a homogenizer to obtain the mixed solution, wherein the homogenizing pressure is 15 Mpa;

step five: sterilizing, namely pre-sterilizing the mixed solution homogenized in the step four, wherein the sterilization temperature is 110 ℃, and the sterilization time is 8 s;

step seven: spray drying, namely feeding the mixed solution concentrated in the step six into a spray drying tower for spray drying to obtain first mixed powder, wherein the spray pressure is 14MPa, the air inlet temperature is 165 ℃, and the air exhaust temperature is 80 ℃;

step eight: fluidized bed drying, namely performing secondary drying on the spray-dried first mixed powder by using a fluidized bed to obtain second mixed powder;

and step ten, packaging, namely packaging by vacuum nitrogen charging.

Example 2

The compound grease simulating breast milk fatty acid is prepared by mixing the following components in percentage by weight: sheep milk fat 37%, OPO 30%, palm oil 19%, sunflower seed oil 1%, and soybean oil 15%

An infant formula goat milk powder containing the compound grease simulating breast milk fatty acid comprises the following components: 300 parts of defatted goat milk powder, 410 parts of desalted goat whey powder, 256 parts of liposome, 2 parts of docosahexaenoic acid, 2.8 parts of arachidonic acid, 20 parts of fructo-oligosaccharide, 10 parts of galacto-oligosaccharide, 3 parts of vitamin premix, 2 parts of mineral premix, 2.8 parts of calcium carbonate, 2.3 parts of choline, 0.22 part of L-carnitine, 0.44 part of nucleotide and 0.08 part of lutein.

Preferably, the vitamin premix is prepared by mixing 55% of ascorbic acid, 9% of dl- α -tocopheryl acetate, 4.73% of nicotinamide, 1.6% of D-calcium pantothenate, 0.16% of thiamine hydrochloride, 0.45% of pyridoxine hydrochloride, 0.8% of retinyl acetate, 0.2% of folic acid, 0.0477% of phytomenadione, 0.0095% of biotin, 0.001% of cholecalciferol, 0.0018% of cyanocobalamin and 28% of inositol.

Preferably, the mineral premix is prepared by mixing the following components in percentage by weight: 35.6% of calcium carbonate, 15.34% of calcium chloride, 17.6% of sodium citrate, 17.68% of potassium citrate, 11% of magnesium chloride, 2% of ferrous sulfate, 0.65% of zinc sulfate, 0.12% of copper sulfate, 0.032% of manganese sulfate, 0.03% of sodium selenite and 0.02% of potassium iodide.

A preparation method of liposome simulating breast milk fat globule structure comprises the following steps.

1) Dissolving a certain amount of MFGM concentrated whey protein in Milli-Q water at a mass percent of 2%, and magnetically stirring in a water bath (750rpm, 45 ℃) for 16 hours;

2) adjusting the pH value of the polar lipid solution to 6.7 by using 0.5mol/L and 5mol/L sodium hydroxide solution;

3) then adding the compounded vegetable oil pocket into the protein solution (the ratio of the oil pocket to the protein solution is 2: 8) all samples were treated with a shear at 7000rpm/min for 8 minutes;